Consulta: subjectFacets:"Anticancer"
Registros recuperados: 11
Data/hora: 02/06/2024 04:53:12

Anticancer and apoptosis-inducing activities of microbial metabolites
Provedor de dados: 69
Autores: Phonnok,Sirinet; Uthaisang-Tanechpongtamb,Wanlaya; Wongsatayanon,Benjamas Thanomsub.
The problems of systemic toxicity and drug resistance in cancer chemotherapy urge the continuing discovery of new anticancer agents. We explored the specific anticancer activity from microbial metabolites to find new lead compound. 394 microbial extracts were evaluated on anti-proliferative activity against 4 cancer cell lines using MTT assay. Of these, 20 samples showed varying degree of cytotoxicity but specifically to the cancer cell lines since the growth of normal cells was not significantly inhibited by 1 mg/ml of each cell extracts. The 4 most potent extracts exhibited strongest growth inhibition to each cancer cell type were selected for further studied. Cell morphological changes such as cell shrinkage, lose of surface contact and blebbing were...
Tipo: Journal article
Palavras-chave: Anticancer; Apoptosis; Bioactive compound; Caspase; Microbial product.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500001

Cytotoxic effect of ICD-85 (venom-derived peptides) on MDA-MB-231 cell line
Provedor de dados: 80
Autores: Zare Mirakabadi,A; Mahdavi,S; Koohi,MK; Taghavian,M.
Since 1987, when chemopreventive testing programs began, more than 1,000 agents and agent combinations have been selected and evaluated in preclinical studies of chemopreventive activity against various types of cancers. In the present study we aimed to provide quantitative and qualitative characterization of biological and pharmacological activities of ICD-85 on MDA-MB-231 cell line (a highly invasive breast cancer cell line) in order to gain a better understanding of the cytotoxic and apoptotic effects of this compound. For this study, the MDA-MB-231 cell line was used and the effect of ICD-85 was assayed by measuring the activity of the cytosolic enzyme lactate dehydrogenase (LDH), released into the culture medium after membrane damage. Morphological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom peptides; MDA-MB-231; Anticancer; Necrosis; Cytotoxic.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400005

Anticancer potential of nanogold conjugated toxin GNP-NN-32 from Naja naja venom
Provedor de dados: 80
Autores: Attarde,Saurabh S.; Pandit,Sangeeta V.
Abstract  Background: Cancer is the second most common fatal disease in the world, behind cardiovascular disorders in the first place. It accounts for around 0.3 million deaths per year in India due to the lack of proper diagnostic facilities, prevention and treatment. Current therapeutic methods do not provide adequate protection and affect normal cells along with cancerous ones. Thus, there is a need for some alternative therapeutic strategy, preferably from natural products, which have been traditionally used for treatment of various diseases in the country.  Methods: In this study, we have conjugated purified NN-32 toxin from Naja naja venom with gold nanoparticles and its anticancer potential was evaluated against human breast cancer cell lines....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GNP-NN-32; Anticancer; Toxin; Naja naja; Venom; Breast cancer; MCF-7; MDA-MB-231.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100304

Anticancer properties of phospholipase A2 fromDaboia siamensis venom on human skin melanoma cells
Provedor de dados: 80
Autores: Khunsap,Suchitra; Khow,Orawan; Buranapraditkun,Supranee; Suntrarachun,Sunutcha; Puthong,Songchan; Boonchang,and Supatsorn.
Abstract  Background  Phospholipase A2 (PLA2) is a major component of theDaboia siamensis venom, which is able to hydrolyse the membrane of various cells. For this reason, the activity of PLA2was investigated regarding its pharmaceutical properties. This study was conducted to explore the pharmacological properties of a PLA2from Daboia siamensis (dssPLA2) venom on human skin melanoma cell line (SK-MEL-28).  Methods  dssPLA2 was isolated by ion exchange and gel filtration columns. Various concentrations of dssPLA2were investigated for cytotoxic activity and inhibition of migration on SK-MEL-28 cells. Cell death analysis, mRNA expression levels of Notch I-III and BRAF V600E genes were also determined.  Results  dssPLA2 exhibited cytotoxicity on SK-MEL-28 for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phospholipase A2; Daboia siamensis venom; Skin melanoma; Anticancer.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100305

Deacylative alkylation (DaA) of N-methyl-3-acetyl-2-oxindole for the synthesis of symmetrically 3,3-disubstituted 2-oxindoles. An access gate to anticancer agents and natural products.
Provedor de dados: 42
Autores: MORENO-CABRERIZO,CRISTINA; ORTEGA-MARTÍNEZ,AITOR; MOLINA,CYNTHIA; NÁJERA,CARMEN; SANSANO,JOSÉ M.
ABSTRACT The synthesis of 3,3-disubstituted N-methyloxindoles, starting from 3-acetyl-2-hydroxy-1-methyloxindole employing a sequential one-pot synthesis, is studied. The process involves a first alkylation in the presence of 1 equiv. of both organic halide and Triton B and the second one employs another 1.5 equiv. of each in moderate to high yields. This procedure is compared with the results obtained from the direct dialkylation of N-methyloxindole. The metathesis of one of the corresponding diallylated product was also studied obtaining the spiranic oxindole. All these methodologies are directed towards the access to anticancer agents and natural biologically active products.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deacylation; Alkylation; 2-oxindoles; Metathesis; Anticancer; Natural products.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000301089

Ultrastructural changes of erythrocytes in whole blood after exposure to prospective in silico-designed anticancer agents: a qualitative case study
Provedor de dados: 48
Autores: Repsold,Lisa; Mqoco,Thandi; Wolmarans,Elize; Nkandeu,Sandra; Theron,Joji; Piorkowski,Tomek; du Toit,Peet; van Papendorp,Dirk; Joubert,Annie Margaretha.
BACKGROUND: Novel, in silico-designed anticancer compounds were synthesized in our laboratory namely, 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10),15-tetraen-17-ol (ESE-15-ol) and 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10)16-tetraene (ESE-16). These compounds were designed to have improved bioavailability when compared to their source compound, 2-methoxyestradiol. This theoretically would be due to their increased binding affinity to carbonic anhydrase II, present in erythrocytes. Since the novel compounds under investigation are proposed to be transported within erythrocytes bound to carbonic anhydrase II, the morphological effect which they may exert on whole blood and erythrocytes is of great significance. A secondary outcome included revision of previously...
Tipo: Journal article
Palavras-chave: Whole blood; Morphology; ESE-15-ol; ESE-16; Anticancer; Erythrocytes.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100039

Biological properties of Endophytic Fungi
Provedor de dados: 52
Autores: Sudha,Venkatesan; Govindaraj,Ramar; Baskar,Kathirvelu; Al-Dhabi,Naif Abdullah; Duraipandiyan,Veeramuthu.
The endophytic fungus is have rich source of secondary metabolites which act as biological active agent in the higher plants. The fungal derivatives play vital part in human life and their compounds are the source of drug for cancer, microbial and viral diseases. The natural compounds from the endophytes acts as growth inhibitor of plant pathogenic organism. Endophytes are rich sources of natural products which are used in agriculture (plant growth and insecticidal), pharmaceutical industries and also used for phytoremediation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic fungi; Secondary metabolites; Anticancer; Phytoremediation; Pest management.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100413

Chemical profile, agaritine and selenium content of Agaricus bisporus
Provedor de dados: 52
Autores: Mohamed,Eman Mostafa.
Chemical profile of the Agaricus bisporus samples were analyzed using GC/MS method in ethanolic extracts. A total of 174 metabolic products were detected, which included 13 significant metabolites between 1.2 to 83 % (w/w); other 13 metabolites at 1% (w/w) and 148 metabolites less than 1% classified into 12 categories. These metabolites had many medicinal activities, which included anti-cancer, anti-cardiovascular diseases, anti-hypercholesterol, anti-microbial, hepatoprotective, human health supporting and immune enhancer. HPLC analysis of water extracts of the A. bisporus samples showed that the spores and gills in the fresh adult mushroom had higher percentage of agaritine mycotoxin than the other parts. These contents were decreased by different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus bisporus; Chemical profile; Agaritine; Selenium; Anticancer; Antihypercholesterol; Aroma compounds; Galvanometric; GC/MS and HPLC.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600015

Screening of Metarhizium spp. strains for anticancer indolizidine alkaloid production and its rapid detection by MS analysis
Provedor de dados: 58
Autores: Justo,Oselys Rodriguez; Calheiros,Chrissana Santos; Perez,Victor Haber; Alegre,Ranulfo Monte.
Six fungi strains (M. anisopliae 3935, 4516, 4819, PL57, PL43 and M. flavoviride CG291) were studied regarding their ability to produce an anticancer indolizidine alkaloid. The culture process was carried out in Shaken flask at 26ºC and 200 rpm using three different culture medium containing oat meal extract supplemented with glucose and DL-lysine or Czapek culture medium. The mycelial extracts produced by Metarhizium spp. cultures were directly submitted to electrospray ionization mass spectrometry (ESI-MS) analysis and the highest alkaloid concentration (approximately, 6 mg.L-1) was reached when M. anisopliae 3935 was tested.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: M. anisopliae; M. flavoviride; Anticancer; Alkaloid; Fermentation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400035

In vitro anticancer activity of microbial isolates from diverse habitats
Provedor de dados: 60
Autores: Thomas,Angel Treasa; Rao,Josyula Venkata; Subrahmanyam,Volety Mallikarjuna; Chandrashekhar,Hariharapura Raghu; Maliyakkal,Naseer; Kisan,Tukaram Kedar; Joseph,Alex; Udupa,Nayanabhirama.
Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticancer; Cytotoxicity; Flow cytometry; Microbial extracts; MTT assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200009

Targeting of temozolomide using magnetic nanobeads: an in vitro study
Provedor de dados: 60
Autores: Gürten,Berna; Yenigül,Elçin; Sezer,Ali Demir; Altan,Cem; Malta,Seyda.
Temozolomide, a chemotherapeutic drug that is often administered for the treatment of brain cancer has severe side effects and a poor aqueous solubility. In order to decrease the detrimental effect of the drug over healthy cells, a novel drug delivery vehicle was developed where the therapeutic drug was encapsulated within the hydrophobic cavities of b-CD modified magnetite nanoparticles, which are embedded in chitosan nanobeads prepared by salt addition. In-vitro studies have shown that the magnetic properties of the novel delivery vehicle are adequate for targeted drug delivery applications under an external magnetic field. Additionally, an increase in the amount of chitosan was shown to exhibit a strong shielding effect over the magnetic properties of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temozolomide; Magnetite; Cyclodextrin; Chitosan; Targeting; Anticancer.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100574