Consulta: subjectFacets:"Antifungal"
Registros recuperados: 35
Data/hora: 16/06/2024 08:52:27

Action mechanisms involved in the bioprotective effect of Lactobacillus harbinensis K.V9.3.1.Np against Yarrowia lipolytica in fermented milk
Provedor de dados: 5
Autores: Mieszkin, Sophie; Hymery, Nolwenn; Debaets, Stella; Coton, Emmanuel; Le Blay, Gwenaelle; Valence, Florence; Mounier, Jerome.
The use of lactic acid bacteria (LAB) as bioprotective cultures can be an alternative to chemical preservatives or antibiotic to prevent fungal spoilage in dairy products. Among antifungal LAB, Lactobacillus harbinensis K.V9.3.1Np showed a remarkable antifungal activity for the bioprotection of fermented milk without modifying their organoleptic properties (Delavenne et al., 2015). The aim of the present study was to elucidate the action mechanism of this bioprotective strain against the spoilage yeast Yarrowia lipolytica. To do so, yeast viability, membrane potential, intracellular pH (pHi) and reactive oxygen species (ROS) production were assessed using flow cytometry analyses after 3, 6 and 10 days incubation in cell-free supernatants. The tested...
Tipo: Text
Palavras-chave: Lactobacillus harbinensis; Yarrowia lipolytica; Bioprotection; Antifungal; Action mechanisms; Dairy products.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00373/48390/48563.pdf

Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris
Provedor de dados: 5
Autores: Rolland, Jean-luc; Abdelouahab, Mahdia; Dupont, J.; Lefevre, F.; Bachere, Evelyne; Romestand, Bernard.
The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenoeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pl=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 mu M &lt; MIC &lt;2.5 mu M), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram () bacteria, Vibrio sp. (40 mu M &lt; MIC &lt;80 mu M). However, rLs-Stylicin1 is able to agglutinate Vibrio pennaeicidae in vitro in agreement with its potent...
Tipo: Text
Palavras-chave: Ls-Stylicin 1; Peptide; Shrimp; Antifungal; Agglutination; Vibrio.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11387/8094.pdf

Detection of griseofulvin in a marine strain of Penicillium waksmanii by ion trap mass spectrometry
Provedor de dados: 5
Autores: Petit, Karina; Mondeguer, Florence; Roquebert, M; Biard, J; Pouchus, Y.
A marine strain of Penicillium waksmanii Zaleski was isolated from a sample of seawater from shellfish-farming area in the Loire estuary (France). The in vitro marine culture showed an important antifungal activity. Bioassay-guided fractionation was used to purify the crude extract. Dereplication by electrospray-ion trap/mass spectrometry (ESI-IT/MS) afforded the identification of the antifungal compound, after a semi-purification consisting of two stages. A comparison of the ionic composition between the active and the non-active fractions allowed the detection of a monocharged ion at m/z 353 containing a chlorine atom, which could be attributed to the antifungal griseofulvin [C17H17ClO6+H](+). Multi-stage fragmentation (MSn) confirmed the identity of the...
Tipo: Text
Palavras-chave: Mass spectrometry ESI IT/MS; Griseofulvin; Antifungal; Marine fungi; Penicillium waksmanii.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-673.pdf

Antimicrobial Compounds from Eukaryotic Microalgae against Human Pathogens and Diseases in Aquaculture
Provedor de dados: 5
Autores: Falaise, Charlotte; Francois, Cyrille; Travers, Marie-agnes; Morga, Benjamin; Haure, Joel; Tremblay, Rejean; Turcotte, Francois; Pasetto, Pamela; Gastineau, Romain; Hardivillier, Yann; Leignel, Vincent; Mouget, Jean-luc.
The search for novel compounds of marine origin has increased in the last decades for their application in various areas such as pharmaceutical, human or animal nutrition, cosmetics or bioenergy. In this context of blue technology development, microalgae are of particular interest due to their immense biodiversity and their relatively simple growth needs. In this review, we discuss about the promising use of microalgae and microalgal compounds as sources of natural antibiotics against human pathogens but also about their potential to limit microbial infections in aquaculture. An alternative to conventional antibiotics is needed as the microbial resistance to these drugs is increasing in humans and animals. Furthermore, using natural antibiotics for...
Tipo: Text
Palavras-chave: Biological activity; Antimicrobial; Antibacterial; Antifungal; Antiviral; Biological activity; Haslea; Microalgae; Sustainable aquaculture.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00349/46052/45719.pdf

Identification of antifungal compounds from slender amaranth
Provedor de dados: 87
Autores: Akbar,Muhammad; N. Sherazi,Iram; Khalil,Tayyaba; S. Iqbal,Muhammad; Akhtar,Shamim; N. Khan,Salik.
Abstract  Background:  Antifungal activity of slender amaranth (Amaranthus viridis L.) is well documented but such studies are scarce in Pakistan, especially against plant pathogens. It was hypothesized that A. viridis has antifungal activity against fungal phyto-pathogens also.  Objective:  Identification of antifungal constituents from leaf extracts of A. viridis.  Methods:  Different organic solvent extracts of A. viridis leaves were evaluated against 5 plant pathogenic fungal species viz. Alternaria alternata, Aspergillus flavus, Drechslera australiensis, Fusarium oxysporum and Macrophomina phaseolina. Antifungal activity of A. viridis was determined by serial dilution method. Six levels (0, 5, 10, 15, 20 and 25 mg mL-1) of treatments of each n-hexane,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amaranthus viridis; Antifungal; GC-MS; Plant pathogenic; Slender amaranth.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100313

BIOEFFICACY OF A COMMON WEED Datura metel AGAINST Colletotrichum gloeosporioides
Provedor de dados: 87
Autores: KARIM,M.; JABEEN,K.; IQBAL,S.; JAVAID,A.
ABSTRACT Mango (Mangifera indica) is one of the most important trees grown mainly in the tropical and subtropical countries. Anthracnose disease caused by Colletotrichum gloeosporioides, is the most damaging disease causing reduction of flower set and yield losses in mango. The present study was designed to evaluate antifungal activity of Datura metel extracts against C. gloeosporioides. Extracts (1% to 3%) of different parts of D. metel viz. leaves, seeds and roots were assessed for their antifungal potential. Methanolic seed extract of D. metel depicted the highest antifungal potential against the test fungus. All the applied concentrations effectively reduced growth of the test fungus. However, 1.5% conc. of methanolic seeds extract showed maximum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; GC-MS; Methanolic extracts; Phytochemical analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100244

In-Vitro Antibacterial and Antifungal Effects of High Levels of Chinese Star Anise
Provedor de dados: 89
Autores: Alhajj,MS; Qasem,MAA; Nabi,AR Jar El; Al-Mufarrej,SI.
ABSTRACT This study investigated the in-vitro antibacterial and fungicidal activities of three crude extracts of Illicium verum (absolute methanol, 50% methanol and aqueous extracts) against two Gram-positive bacteria (Staphylococcus aureus and Listeria monocytogenes), two Gram-negative bacteria (Escherichia coli and Salmonella Arizona), and two fungi (Aspergillus fumigatus and Aspergillus niger). The antibacterial action was measured using an agar disk diffusion test, and the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined. The fungicidal activity of the star anise extracts was assessed by the agar disk diffusion test, and poisoned food techniques, and the MICs and minimum fungicidal concentrations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Illicium verum; Antibacterial; Antifungal.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100330

Antifungal activity of acidic electrolyzed water against strawberry postharvest molds (Fragaria x ananassa Duch cv. Camarosa)
Provedor de dados: 109
Autores: Guerra-Sierra,Beatriz Elena; Sandoval-Meza,Adriana Ximena; García-Sanchéz,Liliana Torcoroma.
Abstract The aim of this word was to assess the in vitro antifungal activity of acidic electrolyzed water (AEW) and the required contact time to inhibit the development of post-harvest molds common in strawberry fruit. Ninety- four strawberry samples were used. A five-fold assay in a randomized design was carried out using standard inoculum of fungal conidia, which were subjected to an array of contact times and AEW concentrations, using a controlled agitation (120 r.p.m) at 23°C. The conidia treated with AEW were grown on potato dextrose agar and their germination was evaluated. Mycelial growth of the treated samples was measured and compared against conidia control samples treated with distilled water instead of AEW. Botrytis cinerea and Rhizopus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acidic electrolyzed water; Antifungal; Botrytis cinerea; Post-harvest molds; Rhizopus stolonifer.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122019000200126

Antimicrobial activity of honeys from two stingless honeybee species and Apis mellifera (Hymenoptera: Apidae) against pathogenic microorganisms
Provedor de dados: 36
Autores: Cruz,Carolinie Batista Nobre da; Pieri,Fabio Alessandro; Carvalho-Zilse,Gislene Almeida; Orlandi,Patrícia Puccinelli; Nunes-Silva,Carlos Gustavo; Leomil,Luciana.
Honeys are described possessing different properties including antimicrobial. Many studies have presented this activity of honeys produced by Apis mellifera bees, however studies including activities of stingless bees honeys are scarce. The aim of this study was to compare the antimicrobial activity of honeys collected in the Amazonas State from Melipona compressipes, Melipona seminigra and Apis mellifera against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Chromobacterium violaceum, and Candida albicans. Minimum inhibitory concentrations were determined using the agar dilution method with Müller-Hinton agar (for bacteria) or Saboraud agar (for yeast). Staphylococcus aureus and E. faecalis were inhibited by all honeys at concentrations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona; Antibacterial; Antifungal; Natural products; Functional food.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672014000200015

Antimicrobial activity of bergenin from Endopleura uchi (Huber) Cuatrec
Provedor de dados: 36
Autores: Silva,Saulo L. da; Oliveira,Viviane Guedes de; Yano,Tomomasa; Nunomura,Rita de Cássia Saraiva.
Endopleura uchi (Huber) Cuatrec. is an Amazon species traditionally used as treatment for inflammations and female disorders. Bergenin was isolated from ethyl acetate fraction of bark of E. uchi by using column chromatography over sephadex LH-20 and then silica gel 60 flash. Its structure was identified on the basis of its NMR spectra. The antimicrobial activity of bergenin and fractions of methanol extract of E. uchi were evaluated against ATCC microorganisms (Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Candida albicans, C. guilliermondii, Aspergillus flavus, A. nidulans). Clinically isolated strains of all of these microorganisms, along with C. tropicalis, A. niger, Shigella sonnei,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial; Antifungal; Candida; Humiriaceae; Uxi-amarelo.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672009000100019

ANTIFUNGAL ACTIVITY SCREENING OF ANTARCTIC ACTINOBACTERIA AGAINST PHYTOPATHOGENIC FUNGI
Provedor de dados: 37
Autores: SANTOS,Andrés; NÚÑEZ-MONTERO,Kattia; LAMILLA,Claudio; PAVEZ,Mónica; QUEZADA-SOLÍS,Damián; BARRIENTOS,Leticia.
ABSTRACT The extreme weather conditions in the Antarctic have exerted selective pressures favoring differential features in bacteria to survive this untapped environment (i.e., antibiotic molecules). Notably, higher chances of antibiotic discovery from extremophiles have been proposed recently. Although new organic and environmentally friendly sources for helping in the control of plant pathogenic fungi are necessary, the information about anti-phytopathogenic applications of extremophile microorganisms from untapped environments is limited. In this study, we determined the antifungal effect of actinobacterial strains isolated from Antarctic soils and sediments. Co-culture inhibition assays and Minimum Inhibitory Concentration (MIC) determination revealed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antagonism; Antarctic bacteria; Antifungal; Extremophile; Plant pathogen.
Ano: 2020
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000200353

Evaluation of in vitro biological properties of Senna occidentalis (L.) Link
Provedor de dados: 3
Autores: Lombardo, Márcia; Kiyota, Sumika; Kato, Edna Tomiko Miyake; Mathor, Monica Beatriz; Pinto, Terezinha de Jesus Andreoli; Kaneko, Telma Mary.
Senna species have been widely used by American, African and Indian ethic groups mainly in the treatment of feebleness, constipation, liver disorders and skin infections. Senna occidentalis (L.) Link is a perennial shrub native to South America and indigenous to tropical regions throughout the world. Current study evaluated the antimicrobial activity of aqueous and hydroalcoholic extracts from S. occidentalis prepared from different parts of the plant. Antimicrobial activity was assessed against standard pharmaceutical microorganisms by spectrophotometry and microdilution technique. Escherichia coli was sensitive only to compounds extracted from seeds which may be proteinaceous. A broader antimicrobial spectrum was demonstrated by the hydroalcoholic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.10.06.00-8 Cassia occidentalis; Leguminosae; Traditional medicine; Antibacterial; Antifungal; Fibroblasts Etnofarmacologia.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/22525

Antifungal susceptibility of yeasts isolated from anthropogenic watershed
Provedor de dados: 42
Autores: MILANEZI,ANA C.M.; WITUSK,JOÃO P.D.; VAN DER SAND,SUELI T.
Abstract Yeasts are unicellular fungi widespread in the environment, and studies suggest that there is a positive correlation between yeast and polluted aquatic environments. The aim of this study was to analyze the diversity and resistance of yeasts isolated from water samples collected along the Arroio Dilúvio in Porto Alegre to antifungals. Yeast isolates from the Arroio Dilúvio were subjected to susceptibility assays against antifungals using the minimum inhibitory concentration (MIC) test, and amplification of the ITS1-5.8S-IT2 region; in addition, the ITS-5.8S region was sequenced for some of the isolates. The amplification product was subjected to PCR-RFLP, and the restriction profile allowed the construction of a dendrogram. Susceptibility tests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Antifungal; Watershed; Resistance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100601

Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils
Provedor de dados: 42
Autores: PINTO,MARIA E.A.; ARAÚJO,STHÉFANE G.; MORAIS,MARCELA I.; SÁ,NÍVEA P.; LIMA,CAROLINE M.; ROSA,CARLOS A.; SIQUEIRA,EZEQUIAS P.; JOHANN,SUSANA; LIMA,LUCIANA A.R.S.
ABSTRACT Fatty acid methyl esters (FAMEs) were obtained from vegetable oils of soybean, corn and sunflower. The current study was focused on evaluating the antifungal activity of FAMEs mainly against Paracoccidioides spp., as well as testing the interaction of these compounds with commercial antifungal drugs and also their antioxidant potential. FAMEs presented small IC50 values (1.86-9.42 μg/mL). All three FAMEs tested showed antifungal activity against isolates of Paracoccidioides spp. with MIC values ranging from 15.6-500 µg/mL. Sunflower FAMEs exhibited antifungal activity that extended also to other genera, with an MIC of 15.6 μg/mL against Candida glabrata and C. krusei and 31.2 μg/mL against C. parapsilosis. FAMEs exhibited a synergetic effect with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Antioxidant; Fatty acid methyl esters; Vegetable oils.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401671

In vitro cytotoxic, antifungal, trypanocidal and leishmanicidal activities of acetogenins isolated from Annona cornifolia A. St. -Hil. (Annonaceae)
Provedor de dados: 42
Autores: LIMA,LUCIANA A.R.S.; ALVES,TÂNIA M.A.; ZANI,CARLOS L.; SALES JÚNIOR,POLICARPO A.; ROMANHA,ALVARO J.; JOHANN,SUSANA; CISALPINO,PATRÍCIA S.; PIMENTA,LÚCIA P.S.; BOAVENTURA,MARIA AMÉLIA D.
Annona cornifolia A. St. -Hil. is a small annual perennial tree found in the Brazilian savannah; their green fruit is popularly used in the treatment of ulcers. The acetogenins isolated from the seeds of Annona cornifolia previously showed to possess antioxidant activity. In continuation of our investigations on the biological activities of acetogenins, four binary mixtures and ten pure adjacent bis-tetrahydrofuran annonaceous acetogenins were evaluated: the cytotoxic (against three human tumor cell lines), antifungal (against Paracoccidioides brasiliensis), trypanocidal (against Trypanosoma cruzi) and leishmanicidal (against Leishmania amazonensis) activities. Acetogenins presented cytotoxic activity confirming their potential use in anti-cancer therapy....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annona cornifolia; Acetogenins; Cytotoxic; Antifungal; Trypanocidal; Leishmanicidal.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200829

Development of modified medium for the enhancement in antifungal activity of P. steckii (MF1 mangrove fungi) against verticillium wilt pathogenic fungi of rose
Provedor de dados: 52
Autores: Sabat,Jyotasanmayee; Gupta,Nibha.
The antifungal activity of Penicillium steckii (MF1) against pathogenic fungi of rose causing verticillium wilt was evaluated under differential nutrient condition. A suitable and modified medium was obtained in which test fungus exhibited enhanced antifungal activity and produced larger inhibition zone against the verticillium fungus. The study carried out to modify the medium components at level of nitrogen, carbon source and their concentration on agar plates. Addition of amino acids, metals and plant growth hormones did not show positive effect but glycerol enhanced the antifungal activity very much.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Penicillium; Nutrient; Rose; Verticillium wilt.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400003

Applications of Plackett–Burman and Central Composite Design for the Optimization of Novel Brevundimonas diminuta KT277492 Chitinase Production, Investigation of its Antifungal Activity
Provedor de dados: 52
Autores: Warda,E. Ashour; Abeer,A. Abd El Aty; Eman,R. Hamed; Mahmoud,A. Swelim; Ahmed,I. El-Diwany.
ABSTRACT Biological control strategy which can damage chitin, a vital component of pathogenic fungi and arthropods promises a safe solution for many fungal problems. And it’s more favorable than chemicals which increase health risks and environmental problems. Thus, the chitinase producers appear potential candidates of biological control of pathogenic fungi. Brevundimonus diminuta KT277492 is a new isolate that has been isolated recently from Egyptian soil. Significant factors that affecting the chitinase enzyme production were studied and optimized using Plackett-Burman and Response Surface Methodology (RSM). As a result, maximum production of chitinase enzyme was 832.87 IUL-1, this result presented about 8.767-fold increase in the enzyme production. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitinase; Brevundimonas diminuta KT277492; Optimization; Plackett–Burman; Central composite design; Antifungal.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100429

In Vitro antifungal potency of plant extracts against five phytopathogens
Provedor de dados: 52
Autores: Tapwal,Ashwani; Nisha,; Garg,Shipra; Gautam,Nandini; Kumar,Rajesh.
The antifungal activity of aqueous extract of Cannabis sativa, Parthenium hysterophorus, Urtica dioeca, Polystichum squarrosum and Adiantum venustum was investigated against Alternaria solani, Alternaria zinniae, Curvularia lunata, Rhizoctonia solani and Fusarium oxysporum at different concentrations (5, 10, 15 and 20%). At 20%, maximum antifungal potential was observed with the extracts of C. sativa, which recorded excellent inhibitory activity against C. lunata (100%), A. zinniae (59.68%), followed by leaf extract of P. hysterophorus (50%) against A. solani. The application of botanical extracts for disease management could be less expensive, easily available, non-polluting and eco-friendly.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Botanical; Phytoextract; Antifungal; Phytopathogen; IPM.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600003

Phytochemical and antifungal activity of anthraquinones and root and leaf extracts of Coccoloba mollis on phytopathogens
Provedor de dados: 52
Autores: Barros,Iuri Bezerra de; Daniel,Juliana Feijó de Souza; Pinto,Jurandir Pereira; Rezende,Maria Inês; Braz Filho,Raimundo; Ferreira,Dalva Trevisan.
The aim of this work was to study the phytochemical and antifungal activity of anthraquinones and root and leaf extracts of Coccoloba mollis on phytopathogens. The chemical analysis of ethanolic extracts showed a mixture of long-chain hydrocarbons, carboxyl esters and 3-taraxerone in the leaf extract. Two anthraquinones (emodin and physcion) were isolated and identified from the root extract. Phytochemical screening using the pharmacognostic methods revealed the presence of flavonoids and tannins in the leaves and roots. Anthraquinones were only found in the root extract, no alkaloids, coumarins, saponins and simple phenolics were present. The antifungal activity of C. mollis extracts and anthraquinones isolated from the root of this plant against...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Phytochemical; Lasiodiplodia; Botryosphaeria; Coccoloba mollis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300015

Chloroquine sensitizes biofilms of Candida albicans to antifungal azoles
Provedor de dados: 55
Autores: Shinde,Ravikumar Bapurao; Raut,Jayant Shankar; Chauhan,Nitin Mahendra; Karuppayil,Sankunny Mohan.
Biofilms formed by Candida albicans, a human pathogen, are known to be resistant to different antifungal agents. Novel strategies to combat the biofilm associated Candida infections like multiple drug therapy are being explored. In this study, potential of chloroquine to be a partner drug in combination with four antifungal agents, namely fluconazole, voriconazole, amphotericin B, and caspofungin, was explored against biofilms of C. albicans. Activity of various concentrations of chloroquine in combination with a particular antifungal drug was analyzed in a checkerboard format. Growth of biofilm in presence of drugs was analyzed by XTT-assay, in terms of relative metabolic activity compared to that of drug free control. Results obtained by XTT-metabolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Biofilm; Candida albicans; Combination; Chloroquine; Drug resistance.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400002

Acute disseminated paracoccidioidomycosis in a 3 year-old child
Provedor de dados: 55
Autores: Grossklaus,Daciene de Arruda; Tadano,Tomoko; Breder,Sandra Assis; Hahn,Rosane Christine.
Clinical cases involving paracoccidioidomycosis in children, diagnosed in Mato Grosso State, in the central western region of Brazil, are rare despite the state being classified with a moderate to high incidence. We describe a clinical case of infant acute disseminated Paracoccidioidomycosis in Mato Grosso State, Brazil, highlighting the importance of early differential diagnosis from other severe pathologies, thus contributing to the survival of compromised patients. A 3 year-old male child, weighing 12.8 Kg, originating from Vila Rica, MT, Brazil. The patient presented intermittent 40ºC fever evolving over 40 days, dry cough and painless bilateral cervical adenomegaly, showing no signs of inflammation. This was associated with diarrhea, distension and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Acute paracoccidioidomycosis; Children; Antifungal.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300018

Fluconazole prophylaxis in preterm infants: a systematic review
Provedor de dados: 55
Autores: Rios,Juliana Ferreira da Silva; Camargos,Paulo Augusto Moreira; Corrêa,Luísa Petri; Romanelli,Roberta Maia de Castro.
ABSTRACT  Objective: This article aims to review the use of antifungal prophylaxis with intravenous fluconazole in premature newborns and the occurrence of Invasive Candidiasis.  Methods: This is a systematic review with search at databases: PubMed, Capes Portal, Virtual Health Library (BVS - Biblioteca Virtual em Saúde)/Lilacs, Scopus and Cochrane. The keywords used were: "Antifungal", "Candida" "Fluconazole prophylaxis" and "Preterm infants".  Results: Invasive Candidiasis was evaluated in all the twelve items. In eleven of them, there was a statistically significant difference between the groups receiving prophylactic fluconazole, with lower frequency of Invasive Candidiasis, compared to placebo or no prophylaxis group. Colonization by Candida species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Candida; Fluconazole prophylaxis; Preterm infants.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300333

In-vitro antimicrobial activity of Psidium guajava l. leaf extracts against clinically important pathogenic microbial strains
Provedor de dados: 58
Autores: Nair,Rathish; Chanda,Sumitra.
The methanol, acetone and N, N-dimethylformamide (DMF) fractions of leaves of Psidium guajava L. were evaluated for antibacterial and antifungal activity. Piperacillin and gentamicin were used as standards for antibacterial assay, while nystatin and flucanazole were used as standards for antifungal assay. 91 clinically important strains were used for the study which were both clinical isolates as well as identified strains. The antibacterial activity was more pronounced against gram-positive bacterial and fungal strains. Moderate activity was shown against the gram-negative bacterial strains studied.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial; Antifungal; Psidium guajava extracts; Clinical isolates.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300013

Prevalence of Candida spp. in cervical-vaginal samples and the in vitro susceptibility of isolates
Provedor de dados: 58
Autores: Brandolt,Tchana Martinez; Klafke,Gabriel Baracy; Gonçalves,Carla Vitola; Bitencourt,Laura Riffel; Martinez,Ana Maria Barral de; Mendes,Josiara Furtado; Meireles,Mário Carlos Araújo; Xavier,Melissa Orzechowski.
Abstract Vulvovaginal candidiasis (VVC) is an infection of the genital mucosa caused by different species of the genus Candida. Considering the lack of data on this topic in the south of Brazil, this study aimed to assess the prevalence of Candida spp. in the cervical-vaginal mucosa of patients treated at a university hospital in southern Rio Grande do Sul, as well as the etiology and the susceptibility of the isolates against fluconazole, itraconazole, miconazole and nystatin. Samples were collected at the gynecology clinic of the Federal Hospital of the University of Rio Grande, and the isolates were identified using phenotypic and biochemical tests. The susceptibility analysis was performed according to the CLSI M27-A2 protocol. Of the 263 patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vulvovaginal candidiasis; Antifungal; Susceptibility; Resistance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100145

Antifungal activity against Cryptococcus neoformans strains and genotoxicity assessment in human leukocyte cells of Euphorbia tirucalli L.
Provedor de dados: 58
Autores: Oliveira,Luís Flávio Souza de; Fuentefria,Alexandre Meneghello; Klein,Fernanda da Silva; Machado,Michel Mansur.
In the last times, focus on plant research has increased all over the world. Euphorbia tirucalli L., a plant known popularly as Aveloz, and originally used in Africa, has been drawing attention for its use in the United States and Latin America, both for use as an ornamental plant and as a medicinal plant. E. tirucalli L. is a member of the family Euphorbiaceae and contains many diterpenoids and triterpenoids, in particular phorbol esters, apparently the main constituent of this plant, which are assumed to be responsible for their activities in vivo and in vitro. The in vitro antifungal activities of Euphorbia tirucalli (L.) against opportunistic yeasts were studied using microbroth dilution assay. The results showed that aqueous extract and latex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbia tirucalli L.; Aveloz; Antifungal; Cryptococcus neoformans.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400027

Efficacy of terbinafine and itraconazole on a experimental model of systemic sporotrichosis
Provedor de dados: 58
Autores: Meinerz,Ana Raquel Mano; Xavier,Melissa O.; Cleff,Marlete Brum; Madrid,Isabel Martins; Nobre,Márcia Oliveira; Meireles,Mário Carlos Araújo; Mello,João Roberto Braga de.
Itraconazole is currently considered the drug of choice to treat the diverse clinical presentation of sporotrichosis. On the other hand terbinafine by virtue of its excellent in vitro activity is under comparative evaluation for its therapeutic potential for a wide range of fungal infections. In this study, our aim was to determine the in vivo efficacy of terbinafine and itraconazole on a experimental model of systemic sporotrichosis. 120 rats Wistar received an injection of 2x10³ S. schenckii cells by via the lateral tail vein. After 3 days the animals were treated with terbinafine (250mg/kg) and itraconazole (100 mg/kg) and their respective diluents. In our model, terbinafine and itraconazole were effective in reducing the number of clinical lesions and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Itraconazole; Terbinafine; Sporotrichosis; Antifungal.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400025

Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol
Provedor de dados: 58
Autores: Rana,Inder Singh; Rana,Aarti Singh; Rajak,Ram Charan.
Antifungal properties of some essential oils have been well documented. Clove oil is reported to have strong antifungal activity against many fungal species. In this study we have evaluated antifungal potential of essential oil of Syzygium aromaticum (L.) against some common fungal pathogens of plants and animals namely, Fusarium moniliforme NCIM 1100, Fusarium oxysporum MTCC 284, Aspergillus sp., Mucor sp., Trichophyton rubrum and Microsporum gypseum. All fungal species were found to be inhibited by the oil when tested through agar well diffusion method. Minimum inhibitory concentration (MIC) was determined for all the species. Column chromatography was performed to separate the eugenol rich fraction from clove oil. Out of seven fractions maximum activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oil; Syzygium aromaticum; Antifungal; Chromatography; Eugenol.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400004

Antimicrobial activity of some medicinal plants from the cerrado of the central-western region of Brazil
Provedor de dados: 58
Autores: Violante,Ivana Maria Póvoa; Hamerski,Lidilhone; Garcez,Walmir Silva; Batista,Ana Lucia; Chang,Marilene Rodrigues; Pott,Vali Joana; Garcez,Fernanda Rodrigues.
Ethanol extracts from six selected species from the Cerrado of the Central-Western region of Brazil, which are used in traditional medicine for the treatment of infectious diseases and other medical conditions, namely Erythroxylum suberosum St. Hil. (Erythroxylaceae), Hyptis crenata Pohl. ex Benth. (Lamiaceae), Roupala brasiliensis Klotz. (Proteaceae), Simarouba versicolor St. Hil. (Simaroubaceae), Guazuma ulmifolia Lam. (Sterculiaceae) and Protium heptaphyllum (Aubl.) March. (Burseraceae), as well as fractions resulting from partition of these crude extracts, were screened in vitro for their antifungal and antibacterial properties. The antimicrobial activities were assessed by the broth microdilution assay against six control fungal strains, Candida...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Antibacterial; Antimicrobial; Artemia salina; Cerrado.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400009

Synergistic action of starch and honey against Candida albicans in correlation with diastase number
Provedor de dados: 58
Autores: Boukraa,Laïd; Benbarek,Hama; Moussa,Ahmed.
To evaluate the synergistic action of starch on the antifungal activity of honey, a comparative method of adding honey with and without starch to culture media was used. Candida albicans has been used to determine the minimum inhibitory concentration (MIC) of five varieties of honey. In a second step, lower concentrations of honey than the MIC were incubated with a set of concentrations of starch added to media to determine the minimum synergistic inhibitory concentration (MSIC). The MIC for the five varieties of honey without starch against C. albicans ranged between 40% and 45% (v/v). When starch was incubated with honey and then added to media, a MIC drop has been noticed with each variety. It ranged between 7% and 25%. A negative correlation has been...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Honey; Starch; Diastase Number; Candida albicans.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100010

Chemical characterization of Lippia alba essential oil: an alternative to control green molds
Provedor de dados: 58
Autores: Glamočlija,Jasmina; Soković,Marina; Tešević,Vele; Linde,Giani Andrea; Colauto,Nelson Barros.
The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure of the main compounds confirmed by ¹H and 13C-NMR spectroscopy. Microdilution assays evaluated the essential oil minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Commercial fungicides Ketoconazole and Bifonazole were used as control....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Essential oil; Neral; Geranial; Lippia alba.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400041

Efficacy of aqueous garlic extract on growth, aflatoxin B1 production, and Cyto-morphological aberrations of Aspergillus flavus, causing human ophthalmic infection: topical treatment of A. flavus keratitis
Provedor de dados: 58
Autores: Ismaiel,Ahmed A.; Rabie,Gamal H.; Kenawey,Saied E.M.; EL-Aal,Marwa A. Abd.
By using agar well diffusion assay, antifungal activity of aqueous extract prepared from Egyptian garlic (Allium sativum L.) was evaluated in vitro against two strains of Aspergillus flavus (OC1 and OC10) causing human ocular infection. The recorded minimum inhibitory concentration (MIC) for growth inhibition of both strains was 3.60 mg/ml. Aqueous garlic extract (AGE) was used in successive in vivo tests as an attempt to cure rabbit's fungal keratitis caused by A. flavus OC1. Findings showed that diluted preparation of AGE was effective topical antifungal agent and succeeded to cure severe A. flavus keratitis in a time course less than 10 days without any observable side effects. Microscopic examination showed that AGE induced deleterious...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus flavus. Garlic; Antifungal; Keratitis; Microscopic examination; Aflatoxin B1.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400017

Antifungal activity of Cymbopogon winterianus jowitt ex bor against Candida albicans
Provedor de dados: 58
Autores: Oliveira,Wylly Araújo de; Pereira,Fillipe de Oliveira; Luna,Giliara Carol Diniz Gomes de; Lima,Igara Oliveira; Wanderley,Paulo Alves; Lima,Rita Baltazar de; Lima,Edeltrudes de Oliveira.
Candida albicans is an opportunistic yeast and a member of the normal human flora that commonly causes infections in patients with any type of deficiency of the immune system. The essential oils have been tested for antimycotic activity and pose much potential as antifungal agents. This work investigated the activity of the essential oil of Cymbopogon winterianus against C. albicans by MIC, MFC and time-kill methods. The essential oil (EO) was obtained by hydrodistillation using a Clevenger-type apparatus. It was tested fifteen strains of C. albicans. The MIC was determined by the microdilution method and the MFC was determined when an aliquot of the broth microdilution was cultivated in SDA medium. The phytochemical analysis of EO showed presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida albicans; Antifungal; Cymbopogon winterianus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200004

Characterization of a chitinase with antifungal activity from a native Serratia marcescens B4A
Provedor de dados: 58
Autores: Zarei,Mandana; Aminzadeh,Saeed; Zolgharnein,Hossein; Safahieh,Alireza; Daliri,Morteza; Noghabi,Kambiz Akbari; Ghoroghi,Ahmad; Motallebi,Abbasali.
Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45ºC. Enzyme was stable in 55ºC for 20 min and at a pH range of 3-9 for 90 min at 25ºC. When the temperature was raised to 60ºC, it might affect the structure of enzymes lead to reduction of chitinase activity. Moreover, the Km and Vmax values for chitin were 8.3 mg/ml and 2.4 mmol/min, respectively. Additionally, the effect of some cations and chemical compounds were found to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Characterization; Chitinase; Serratia marcescens B4A.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300022

Characterization of bacterial endophytes from Myanmar medicinal plants for antimicrobial activity against human and plant pathogens
Provedor de dados: 60
Autores: Myo,Ei Mon; Maung,Chaw Ei Htwe; Mya,Khin Mar; Khai,Aye Aye.
This research aimed to investigate the antagonistic activity of the bacterial endophytes from Myanmar medicinal plants. Thirty-one bacterial isolates were isolated from Myanmar medicinal plants: Tinospora cordifolia (Wild.) Miers., Catharanthus roseus G. Don., Tectona hamiltoniana Wall. and Boscia variabilis Collett &amp; Hemsl. (Capparaceae). Dual culture and agar well diffusion methods were used for antimicrobial assay. One endophyte from Catharanthus roseus and two bacterial isolates from Boscia variabilis Collett &amp; Hemsl. (Capparaceae) had not only the antibacterial activity towards the human pathogenic bacteria but also the antifungal activity against the plant pathogenic fungi. From 16S rRNA sequencing, one strain from Catharanthus roseus G. Don....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial; Antifungal; Culture conditions; Endophytes; Medicinal plants.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100513

Chemical composition, insecticidal, and antifungal activities of fruit essential oils of three colombian Zanthoxylum species
Provedor de dados: 117
Autores: Prieto,Juliet A; Patiño,Oscar J; Delgado,Wilman A; Moreno,Jenny P; Cuca,Luis E.
This study determined the chemical composition of essential oils isolated from Zanthoxylum monophyllum (Lam.) P. Wilson, Z. rhoifolium Lam., and Z. fagara (L.) Sarg. fruits by steam distillation, as well as testing antifungal and insecticidal activities of essential oils as potential pesticides. Gas chromatography-mass spectrometry (GC/MS) analysis identified 57 compounds. The main constituents in Z. rhoifolium oil were &amp;#946;-Myrcene (59.03%), &amp;#946;-phellandrene (21.47%), and germacrene D (9.28%) , the major constituents of Z. monophyllum oil were sabinene (25.71%), 1,8-cineole (9.19%), and cis-4-thujanol (9.19%), whereas fruit oil of Z. fagara mainly contained germacrene D-4-ol (21.1%), elemol (8.35%), and &amp;#945;-cadinol (8.22%). Zanthoxylum...
Tipo: Journal article
Palavras-chave: Fumigant; Antifungal; Insecticidal.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100009