Consulta: subjectFacets:"Arabidopsis"
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Data/hora: 28/05/2024 17:26:03

T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics
Provedor de dados: Electronic Journal of Biotechnology
Autores: Radhamony,Resmi Nath; Mohan Prasad,Anand; Srinivasan,Ramamurthy.
With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidopsis and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool for functional genomics in higher plants. Besides the fact that the insertion of T-DNA element into a gene can lead to loss or gain of function, ingenious use of a variety of vectors have led to the identification of genes and regulatory elements in Arabidopsis. In this review, we highlight the progress made in the field of functional genomics of Arabidopsis using...
Tipo: Journal article
Palavras-chave: Arabidopsis; Functional genomics; Insertional mutagenesis; Promoter trapping; T-DNA tagging.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100012

Introduction of the rd29A: AtDREB2A CA gene into soybean (Glycine max L. Merril) and its molecular characterization in leaves and roots during dehydration
Provedor de dados: Genetics and Molecular Biology
Autores: Engels,Cibelle; Fuganti-Pagliarini,Renata; Marin,Silvana Regina Rockenbach; Marcelino-Guimarães,Francismar Corrêa; Oliveira,Maria Cristina Neves; Kanamori,Norihito; Mizoi,Junya; Nakashima,Kazuo; Yamaguchi-Shinozaki,Kazuko; Nepomuceno,Alexandre Lima.
The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drought. The abscisic acid (ABA)-independent Dehydration Responsive Element Binding (DREB) gene family has been used to obtain plants with increased tolerance to abiotic stresses. In the present study, the rd29A:AtDREB2A CA gene from Arabidopsis thaliana was inserted into soybean using biolistics. Seventy-eight genetically modified (GM) soybean lines containing 2-17 copies of the AtDREB2A CA gene were produced. Two GM soybean lines (P1397 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis; Differential expression; Genetically modified organism; Water deficit.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400015

YUCCA4 overexpression modulates auxin biosynthesis and transport and influences plant growth and development via crosstalk with abscisic acid in Arabidopsis thaliana
Provedor de dados: Genetics and Molecular Biology
Autores: Munguía-Rodríguez,Aarón Giovanni; López-Bucio,Jesús Salvador; Ruiz-Herrera,León Francisco; Ortiz-Castro,Randy; Guevara-García,Ángel Arturo; Marsch-Martínez,Nayelli; Carreón-Abud,Yazmín; López-Bucio,José; Martínez-Trujillo,Miguel.
Abstract Auxin regulates a plethora of events during plant growth and development, acting in concert with other phytohormones. YUCCA genes encode flavin monooxygenases that function in tryptophan-dependent auxin biosynthesis. To understand the contribution of the YUCCA4 (YUC4) gene on auxin homeostasis, plant growth and interaction with abscisic acid (ABA) signaling, 35S::YUC4 seedlings were generated, which showed elongated hypocotyls with hyponastic leaves and changes in root system architecture that correlate with enhanced auxin responsive gene expression. Differential expression of PIN1, 2, 3 and 7 auxin transporters was detected in roots of YUC4 overexpressing seedlings compared to the wild-type: PIN1 was down-regulated whereas PIN2, PIN3 and PIN7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis; Auxin; Abscisic acid; YUCCA4; Root growth; Germination.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100302

Expression analysis of the Arabidopsis thaliana AtSpen2 gene, and its relationship with other plant genes encoding Spen proteins
Provedor de dados: Genetics and Molecular Biology
Autores: Solís-Guzmán,María Gloria; Argüello-Astorga,Gerardo; López-Bucio,José; Ruiz-Herrera,León Francisco; López-Meza,Joel; Sánchez-Calderón,Lenin; Carreón-Abud,Yazmín; Martínez-Trujillo,Miguel.
Abstract Proteins of the Split ends (Spen) family are characterized by an N-terminal domain, with one or more RNA recognition motifs and a SPOC domain. In Arabidopsis thaliana, the Spen protein FPA is involved in the control of flowering time as a component of an autonomous pathway independent of photoperiod. The A. thaliana genome encodes another gene for a putative Spen protein at the locus At4g12640, herein named AtSpen2. Bioinformatics analysis of the AtSPEN2 SPOC domain revealed low sequence similarity with the FPA SPOC domain, which was markedly lower than that found in other Spen proteins from unrelated plant species. To provide experimental information about the function of AtSpen2, A. thaliana plants were transformed with gene constructs of its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spen; Arabidopsis; Vascular bundle; Expression.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400643

A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Lu; Hu,Weijuan; Gao,Yike; Pan,Huitang; Zhang,Qixiang.
Abstract We cloned and characterized the full-length coding sequence of a small heat shock (sHSP) gene, PfHSP17.2, from Primula forrestii leaves following heat stress treatment. Homology and phylogenetic analysis suggested that PfHSP17.2 is a cytosolic class II sHSP, which was further supported by the cytosolic localization of transient expression of PfHSP17.2 fused with green fluorescent protein reporter. Expression analysis showed that PfHSP17.2 was highly inducible by heat stress in almost all the vegetative and generative tissues and was expressed under salt, cold, and oxidative stress conditions as well. Moreover, the expression of PfHSP17.2 in P. forrestii was detected in certain developmental growth stages. Transgenic Arabidopsis thaliana...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SHSPs; Stress tolerance; Arabidopsis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400649

Analysis of the SHP2 enhancer for the use of tissue specific activation tagging in Arabidopsis thaliana
Provedor de dados: Genetics and Molecular Biology
Autores: Chalfun-Junior,Antonio; Mes,Jurriaan J.; Busscher,Marco; Angenent,Gerco C.
Activation tagging is a powerful tool to identify new mutants and to obtain information about possible biological functions of the overexpressed genes. The quadruple cauliflower mosaic virus (CaMV) 35S enhancer fragment is a strong enhancer, which is most commonly used for this purpose. However, the constitutive nature of this enhancer may generate lethal mutations or aberrations in different plant organs by the same overexpressed gene. A tissue-specific activation tagging approach may overcome these drawbacks and may also lead more efficiently to the desired phenotype. For this reason the SHATTERPROOF2 (SHP2) promoter fragment was analysed for enhancer activity. The SHP2 gene is involved in dehiscence zone development and expressed during silique...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis; Development; Pod shattering; Transcription factors; Mutants.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200032

Loci de caracteres cuantitativos asociados con tolerancia a déficit hídrico en Arabidopsis thaliana (L.) Heynh.
Provedor de dados: Phyton: Revista Internacional de Botánica Experimental
Autores: Gutiérrez-Díez,A; Salinas-García,GE; Iracheta-Donjuan,L; Torres-Castillo,JA; Mayek-Pérez,N.
La localización de genes y loci de caracteres cuantitativos (QTLs) en una especie modelo permite conocer la organización de su genoma y la posibilidad para aislar y clonar genes de importancia agronómica. Setenta y tres líneas endogámicas recombinantes (LERs) derivadas de la cruza entre los ecotipos Columbia (Col) x Landsberg erecta (Ler) de Arabidopsis thaliana (L.) Heynh. se cultivaron en invernadero en condiciones de humedad del suelo contrastantes (riego y déficit hídrico). Durante su crecimiento se midieron algunas características fenológicas (días al inicio de la floración) y del crecimiento de las plantas (altura y número de nudos y de hojas por planta). Diferencias significativas en altura y número de nudos por planta se observaron entre LERs. Con...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis; Déficit hídrico; Grupos de ligamiento; Mapa de ligamiento; CLCs.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572013000200007

The importance of Arabidopsis seed mutants in the elucidation of the molecular basis of Endosperm Balance Number in tuberbearing Solanum species
Provedor de dados: Biocell
Autores: Cornejo,P.; Camadro,E.L.; Masuelli,R.W.
The Endosperm Balance Number (EBN) is an important concept for potato breeding and has evolutionary importance in tuber-bearing Solanum species. The EBN is part of the post-zygotic hybridization barriers in the group and represents a reproductive isolating mechanism. Few genes have been proposed to be involved in its genetic control; until now, however, neither specific genes nor its molecular basis have been well established. Histological observations of embryo and endosperm development in inter-EBN crosses in tuber-bearing Solanum revealed phenotypes similar to those recently described in Arabidopsis seed mutants. The common feature between them is that the endosperm nuclei become greatly enlarged and that embryos are arrested at the globular stage. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EBN; Solanum; Endosperm; Arabidopsis; Seed mutants.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300001