Consulta: subjectFacets:"Aspergillus fumigatus"
Registros recuperados: 17
Data/hora: 10/06/2024 15:25:05

Unravelling species boundaries in the Aspergillus viridinutans complex (section Fumigati): opportunistic human and animal pathogens capable of interspecific hybridization
Provedor de dados: 16
Autores: Hubka, V.; Barrs, V.; Dudová, Z.; Sklenář, F.; Kubátová, A.; Matsuzawa, T.; Yaguchi, T.; Horie, Y.; Nováková, A.; Frisvad, J.C.; Talbot, J.J.; Kolařík, M.
Although Aspergillus fumigatus is the major agent of invasive aspergillosis, an increasing number of infections are caused by its cryptic species, especially A. lentulus and the A. viridinutans species complex (AVSC). Their identification is clinically relevant because of antifungal drug resistance and refractory infections. Species boundaries in the AVSC are unresolved since most species have uniform morphology and produce interspecific hybrids in vitro. Clinical and environmental strains from six continents (n = 110) were characterized by DNA sequencing of four to six loci. Biological compatibilities were tested within and between major phylogenetic clades, and ascospore morphology was characterised. Species delimitation methods based on the multispecies...
Tipo: Article / Letter to the editor
Palavras-chave: Aspergillus felis; Aspergillus fumigatus; Invasive aspergillosis; Mating-type genes; Multispecies coalescence model; Neosartorya udagawae; Scanning electron microscopy; Soil fungi.
Ano: 2018
URL: http://www.repository.naturalis.nl/record/652411

Contribution of gliotoxin to aspergillosis
Provedor de dados: 19
Autores: 豊留, 孝仁; TOYOTOME, Takahito.
Aspergillus species (spp.) are medically important pathogens that cause aspergillosis, and A. fumigatus is the most prevalent causative agent of the disease. Aspergillus spp. produce various secondary metabolites; however, the role of these metabolites in aspergillosis remains unclear. Gliotoxin is a major secondary metabolite produced by A. fumigatus. Several recent studies have reported that gliotoxin contributes to the virulence of A. fumigatus. Here I review studies related to gliotoxin and its contribution to the infection caused by A. fumigatus.
Palavras-chave: Aspergillosis; Aspergillus fumigatus; Gliotoxin; Secondary metabolite.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4157

Azole-resistant aspergillus fumigatus containing a 34-bp tandem repeat in cyp51A promoter is isolated from the environment in Japan
Provedor de dados: 19
Autores: Onishi, Kenji; Sarumoh, Bolu Muhammad; Hagiwara, Daisuke; Watanabe, Akira; Kamei, Katsuhiko; Toyotome, Takahito.
Azole-resistant strains of Aspergillus fumigatus containing a tandem repeat in the cyp51A promoter and amino acid substitution(s) have been isolated in the environment worldwide; however, this type of resistant strain had never been isolated from the environment in Japan. Our previous study indicated that an azole-resistant A. fumigatus strain OKH50 containing a 34-bp tandem repeat in cyp51A promoter with L98H substitution in Cyp51A was isolated from a patient in Obihiro of Hokkaido, Japan. In this study, we collected azole-resistant Aspergillus spp. by air sampling from the environment in Japan. One Aspergillus-like colony was isolated from one of 10 sampling sites surveyed. The strain Env1 was confirmed as A. fumigatus by nucleotide sequencing and...
Palavras-chave: Aspergillus fumigatus; Azole; Cyp51A; TR34/L98H.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4565

Azole susceptibility in clinical and environmental isolates of Aspergillus fumigatus from eastern Hokkaido, Japan
Provedor de dados: 19
Autores: Toyotome, Takahito; Fujiwara, Takayuki; Kida, Hideyuki; Matsumoto, Munehiro; Wada, Terumi; Komatsu, Ryoichi.
Azole antifungals are used not only clinically for fungal infections but also used as agricultural fungicides. Recently, azole-resistant Aspergillus fumigatus containing a tandem repeat in the promoter region of cyp51A combined with amino acid substitution(s) appears in the environment in Eurasia, especially in several European countries. Although azole fungicides have been used in Japan, especially in Hokkaido, surveillance and characterization of A. fumigatus in Hokkaido have not been reported. In this study, we collected soil samples from farms that used an azole fungicide in the Tokachi area of eastern Hokkaido, isolated 91 A. fumigatus strains, and determined the minimal inhibitory concentrations of medical azoles required for these strains. Moreover,...
Palavras-chave: Azole; Aspergillus fumigatus; Cyp51A.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4469

Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus Fresenius
Provedor de dados: 59
Autores: Silva,Claudio Henrique Cerri e; Puls,Jurgen; Sousa,Marcelo Valle de; Ferreira Filho,Edivaldo Ximenes.
A xylan-degrading enzyme (xylanase II) was purified to apparent homogeneity from solid-state cultures of Aspergillus fumigatus Fresenius. The molecular weight of xylanase II was found to be 19 and 8.5 kDa, as estimated by SDS-PAGE and gel filtration on FPLC, respectively. The purified enzyme was most active at 55 °C and pH 5.5. It was specific to xylan. The apparent Km and Vmax values on soluble and insoluble xylans from oat spelt and birchwood showed that xylanase II was most active on soluble birchwood xylan. Studies on hydrolysis products of various xylans and xylooligomers by xylanase II on HPLC showed that the enzyme released a range of products from xylobiose to xylohexaose, with a small amount of xylose from xylooligomers, and presented transferase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylan; Xylanase; Aspergillus fumigatus.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200005

Purification and characterization of an alpha-galactosidase from Aspergillus fumigatus
Provedor de dados: 52
Autores: Rezende,Sebastião Tavares de; Guimarães,Valéria Monteze; Rodrigues,Marília de Castro; Felix,Carlos Roberto.
Aspergillus fumigatus secreted invertase (beta-fructofuranosidase) and alpha-galactosidase enzymatic activities able to hydrolyzing raffinose oligosaccharides (RO). alpha-Galactosidase was induced by galactose, melibiose and raffinose, but galactose was the most efficient inducer. It was purified by gel filtration and two ion exchange chromatographies and showed Mw of 54.7 kDa. The purified enzyme showed maximal activity against p-nitrophenyl-alpha-D-galactopyranoside (pNPGal) at pH 4.5-5.5 and 55 °C, and retained about 80% of the original activity after incubation for 90 minutes at 50ºC. The KM for pNPGal was 0.3 mM. Melibiose was hydrolyzed by the enzyme but raffinose was very poor substrate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus fumigatus; Alpha-galactosidase; Raffinose oligosaccharides.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200005

Decolourization of textile dye effluent by non-viable biomass of Aspergillus fumigatus
Provedor de dados: 52
Autores: Kalaiarasi,K.; Lavanya,A.; Amsamani,S.; Bagyalakshmi,G.
The aim of this work was to study the decolourization of textile dye effluent by non-viable biomass of Aspergillus fumigates. The dried non-viable fungal biomass exhibited maximum dye removal at pH 7.0 with temperature of 30ºC and 3 g/l (w/v) biomass concentration, after 24 h contact time. The results showed that the non-viable biomass possessed high efficiency for dye removal from textile effluent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus fumigatus; Decolourization; Textile effluent.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300019

In vitro antifungal susceptibility of clinical and environmental isolates of Aspergillus fumigatus and Aspergillus flavus in Brazil
Provedor de dados: 55
Autores: Denardi,Laura Bedin; Hoch Dalla-Lana,Bianca; Pantella Kunz de Jesus,Francielli; Bittencourt Severo,Cecília; Santurio,Janio Morais; Zanette,Régis Adriel; Alves,Sydney Hartz.
ABSTRACT The in vitro susceptibility of 105 clinical and environmental strains of Aspergillus fumigatus and Aspergillus flavus to antifungal drugs, such as amphotericin B, azoles, and echinocandins was evaluated by the broth microdilution method proposed by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Following the EUCAST-proposed breakpoints, 20% and 25% of the clinical and environmental isolates of A. fumigatus, respectively, were found to be resistant to itraconazole (Minimal Inhibitory Concentration, MIC &gt; 2.0 mg/L). Voriconazole showed good activity against A. fumigatus and A. flavus strains, except for one clinical strain of A. fumigatus whose MIC was 4.0 mg/L. Posaconazole (≤0.25 mg/L) also showed appreciable activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus flavus; Aspergillus fumigatus; Echinocandins; Azoles; Environmental; Susceptibility.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000100030

Cerebral aspergillosis presenting as a mass lesion
Provedor de dados: 55
Autores: Azarpira,Negar; Esfandiari,Majid; Bagheri,Mohamad Hadi; Rakei,Sed; Salari,Soheila.
Fungal infections of the central nervous system (CNS) are almost always a surprising finding. Their presentation is usually subtle, often without any diagnostic characteristics, and they are frequently mistaken for pyogenic abscesses, or brain tumors. Aspergillosis of the central nervous system is an uncommon infection, mainly occurring in immunocompromised patients. It may present in several forms, including meningitis, mycotic aneurysms, infarcts and a tumoral form. We report an intracranial granuloma due to Aspergillus fumigatus involving the anterior cranial fossa and the frontal lobe. The clinical symptoms began one year before admission. Final diagnosis was made after craniotomy. The patient was treated with an extensive excision of the cerebral mass...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Granulomatous inflammation; Aspergillus fumigatus; Fungal infections.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400019

Invasive aspergillosis associated with systemic lupus erythematosus and cardiac postoperative complication
Provedor de dados: 58
Autores: Macêdo,Danielle Patrícia Cerqueira; Silva-Júnior,Heraldo Maia; Souza-Motta,Cristina Maria de; Milan,Eveline Pípolo; Neves,Rejane Pereira.
Aspergillus is a ubiquitous fungus which can cause a variety ofclinical syndromes. This fungus has emerged as agent of systemic infections and has therefore gained considerable public health importance. This paper describes two cases of invasive aspergillosis caused by A. fumigatus in immuno-suppressed patients and underscores the importance of early identification of Aspergillus infection associated with systemic lupus erythematosus and cardiac postoperative complications.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus fumigatus; Opportunistic mycosis; Systemic lupus erythematosus; Cardiac postoperative complications.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100031

Production and partial characterization of serine and metallo peptidases secreted by Aspergillus fumigatus Fresenius in submerged and solid state fermentatio
Provedor de dados: 58
Autores: Silva,Ronivaldo Rodrigues da; Cabral,Tatiana Pereira de Freitas; Rodrigues,André; Hamilton,Cabral.
Enzyme production varies in different fermentation systems. Enzyme expression in different fermentation systems yields important information for improving our understanding of enzymatic production induction. Comparative studies between solid-state fermentation (SSF) using agro-industrial waste wheat bran and submerged fermentation (SmF) using synthetic media were carried out to determinate the best parameters for peptidase production by the fungus Aspergillus fumigatus Fresen. Variables tested include: the concentration of carbon and protein nitrogen sources, the size of the inoculum, the pH of the media, temperature, and the length of the fermentation process. The best peptidase production during SSF was obtained after 96 hours using wheat bran at 30 ºC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metallopeptidase; Serine peptidase; Fermentative parameters; Aspergillus fumigatus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100034

Production of bioethanol using agricultural waste: banana pseudo stem
Provedor de dados: 58
Autores: Ingale,Snehal; Joshi,Sanket J.; Gupte,Akshaya.
India is amongst the largest banana (Musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars. The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to produce...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioethanol; Banana pseudostem; Aspergillus ellipticus; Aspergillus fumigatus; Saccharomyces cerevisiae.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300018

The potential of an Aspergillus fumigatus Brazilian strain to produce antimicrobial secondary metabolites
Provedor de dados: 58
Autores: Furtado,Niege Araçari Jacometti Cardoso; Fonseca,Maria José Vieira; Bastos,Jairo Kenupp.
During the course of screening for biologically leading active natural products from fungi, a strain of Aspergillus fumigatus was assessed for antimicrobial activity production. Four parameters consisting of medium culture, pH, temperature and time of fermentation were investigated. The extracts from the fungus cultures displayed different TLC profiles and produced, at least, one inhibition zone in the bioautography assays. Overlapping inhibition zones in the range of Rf 0.09 to 0.65 against Kocuria rhizophila were detected in the bioautography assay of the chloroform extract obtained from Jackson's medium culture, initial pH of 6.0 and incubation at 40ºC for 144 hours. However, the obtained extracts by developing the fungus culture in Vogel's medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial activity; Aspergillus fumigatus; Bioautography; Fermentation conditions.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400010

Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis
Provedor de dados: 58
Autores: Rungrattanakasin,Budsayachat; Premjet,Siripong; Thanonkeo,Sudarat; Klanrit,Preekamol; Thanonkeo,Pornthap.
Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and encoded a protein of 460 amino acid residues. The predicted molecular weight and the isoelectric point of the A. fumigatus Cel7 gene product were 48.19 kDa and 5.03, respectively. A catalytic domain in the N-terminal region and a fungal type cellulose-binding domain/module in the C-terminal region were detected in the predicted polypeptide sequences. Furthermore, a signal peptide with 20 amino acid residues at the N-terminus was also detected in the deduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus fumigatus; Cellulosic material; Gene cloning; Kluyveromyces lactis; Thermotolerant fungus.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300647

Elastin increases biofilm and extracellular matrix production of Aspergillus fumigatus
Provedor de dados: 58
Autores: Brandão,Ildnay de Souza Lima; Oliveira-Moraes,Heloiza Maria da Silva; Motta,Cristina Maria de Souza; Oliveira,Neiva Tinti de; Magalhães,Oliane Maria Correia.
Abstract Aspergillus fumigatus is an opportunistic saprobe fungus that accounts for 90% of cases of pulmonary aspergillosis in immunosuppressed patients and is known for its angiotropism. When it reaches the respiratory tract, A. fumigatus interacts with structural components and blood vessels of the lungs, such as elastin. To understand the effect of this structural component, we examined the effect of elastin on the production and development of the biofilm of A. fumigatus. In RPMI containing 10 mg/mL of elastin, a significant increase (absorbance p &lt; 0.0001; dry weight p &lt; 0.0001) in the production of biofilm was observed in comparison to when RPMI was used alone, reaching a maximum growth of 18.8 mg (dry weight) of biofilm in 72 h. In addition,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus fumigatus; Biofilm; Elastin; Extracellular matrix; Hydrophobicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300675

Aspergilose em avestruz (Struthio camelus) no Brasil
Provedor de dados: 65
Autores: Paixão,Tatiane Alves da; Nascimento,Ernane Fagundes do; Parra,Paula Namie Shida; Santos,Renato de Lima.
Criatórios de avestruz têm se tornado comuns no Brasil, mas por se tratar de espécie exótica, as informações sobre as doenças desses animais no nosso ambiente são escassas. Um avestruz de cinco meses de idade apresentou tosse, anorexia, perda de peso, dispnéia discreta, temperatura corporal normal e morreu oito dias após o início dos sinais clínicos. Macroscopicamente foram observados nódulos múltiplos no pulmão e nos sacos aéreos. Histolopatogicamente, observou-se pneumonia granulomatosa e necrosante multifocal com hifas ramificadas e septadas intralesionais com características morfológicas de Aspergillus sp. Nos sacos aéreos, além das hifas e processo inflamatório adjacente haviam vários micélios com conidióforos na superfície interna. Foi isolado...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avestruz; Aspergillus fumigatus; Pneumonia; Aerossaculite.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000200037

Aborto e placentite micótica por Aspergillus fumigatus em uma égua
Provedor de dados: 65
Autores: Driemeier,David; Finger,Giovana Peroni; Rocha,Andre Luiz de Araújo; Garbade,Petra; Rodrigues,Reginaldo José Diaz; Mattos,Ricardo Costa.
É descrito um caso de placentite micótica e aborto em uma égua com 7 meses de gestação. Alterações macroscópicas incluíam subdesenvolvido, áreas brancas na placenta e espessamento da membrana cório-alantóide. No pulmão fetal havia duas nodulações com 1 cm de diâmetro. Microscopicamente as lesões placentárias eram predominantemente necrótico-purulentas com focos de mineralização na placenta e trombose com inflamação purulenta no pulmão do feto. Hifas fúngicas septadas e ramificadas estavam presentes na placenta e também nos nódulos pulmonares. Aspergillus fumigatus foi isolado das lesões placentárias. O diagnóstico baseou-se nos achados histopatológicos e no isolamento do agente.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aborto; Doenças de eqüinos; Placentite micótica; Aspergillus fumigatus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781998000200025