Consulta: subjectFacets:"Aspergillus terreus"
Registros recuperados: 9
Data/hora: 01/06/2024 02:36:46

Hydrolysis of galacto-oligosaccharides in soy molasses by α -galactosidases and invertase from Aspergillus terreus
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Reis,Angélica Pataro; Guimarães,Valéria Monteze; Ferreira,Joana Gasperazzo; Queiroz,José Humberto de; Oliveira,Maria Goreti Almeida; Falkoski,Daniel Luciano; Almeida,Maíra Nicolau de; Rezende,Sebastião Tavares de.
Two α -galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28ÂºC were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65ÂºC, respectively. The K M app for Ï -nitrophenyl-α -D-galactopyranoside were 1.32 mM and 0.72 mM for P1 and P2, respectively, while the K M app value for invertase, using sacarose as a substrate was 15.66 mM. Enzyme preparations P1 and P2 maintained their activities after pre-incubation for 3 h at 50ÂºC and invertase maintained about 90% after 6 h at 55 ÂºC. P1 and P2 presented different inhibition sensitivities by Ag+,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus terreus; Galactooligosaccharides; Α-galactosidase; Invertase; Soy molasses.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300029

Peptidase with Keratinolytic Activity Secreted by Aspergillus terreus During Solid-State Fermentation
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Siqueira,Ana Claudia Rodrigues de; Rosa,Nathalia Gonsales da; Motta,Cristina Maria Souza; Cabral,Hamilton.
The aim of this study was to evaluate peptidase production by Aspergillus terreus in solid-state bioprocess and evaluate its parameters. The best conditions were 5.0 g of wheat bran as substrate, incubation temperature 30°C, inoculum 2.0x105spores/g and 75% saline volume, with production reaching 677 U/mL (5400 U/g culture medium) after 72 h of fermentation. Biochemical characterization of the crude enzymatic extract showed the optimum pH and temperature of 6.5 and 55°C, respectively. The stability at different temperatures and pH values showed that the extract could endure different pH. The evaluation of the ions influence and inhibitors proved that the enzyme required an ion for better activity, which was corroborated with the inhibition of EDTA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Solid-state fermentation; Keratinase; Wheat bran; Aspergillus terreus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400514

Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus NCFT 4269.10
Provedor de dados: Brazilian Journal of Microbiology
Autores: Sethi,Bijay Kumar; Nanda,Prativa Kumari; Sahoo,Santilata.
Abstract Enzyme production by Aspergillus terreus NCFT 4269.10 was studied under liquid static surface and solid-state fermentation using mustard oil cake as a substrate. The maximum lipase biosynthesis was observed after incubation at 30 °C for 96 h. Among the domestic oils tested, the maximum lipase biosynthesis was achieved using palm oil. The crude lipase was purified 2.56-fold to electrophoretic homogeneity, with a yield of 8.44%, and the protein had a molecular weight of 46.3 kDa as determined by SDS-PAGE. Enzyme characterization confirmed that the purified lipase was most active at pH 6.0, temperature of 50 °C, and substrate concentration of 1.5%. The enzyme was thermostable at 60 °C for 1 h, and the optimum enzyme–substrate reaction time was 30...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus terreus; Lipase; Liquid static surface fermentation; Solid-state fermentation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100143

Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene
Provedor de dados: Brazilian Journal of Microbiology
Autores: Ahmed,E.M.
In attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11alpha-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30ºC. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4ºC for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 11alpha-hydroxprogesterone; Polytetrafluoroethylene; Aspergillus terreus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200007

Modification of c and n sources for enhanced production of cyclosporin 'a' by Aspergillus Terreus
Provedor de dados: Brazilian Journal of Microbiology
Autores: Tanseer,Sundas; Anjum,Tehmina.
Most of the studies regarding cyclosporin 'A' production through fungi concentrate around Tolypocladium inflatum. This is mainly due to lower reported production of this drug in other fungi. The present study was therefore conducted to explore indigenous isolates of Aspergillus terreus for synthesis of this drug and defining a production medium for obtaining high yield of cyclosporin 'A'. For this purpose carbon and nitrogen sources were optimized for the selected best strain of A. terreus. Overall results depicted that the best cyclosporin 'A' yield from selected Aspergillus terreus (FCBP58) could be obtained by using production medium containing glucose 10% as carbon source and peptone 0.5% as nitrogen source. This modification in production medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus terreus; Cyclosporin 'A'; Carbon; Nitrogen.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400019

Response surface methodology for optimization of production of lovastatin by solid state fermentation
Provedor de dados: Brazilian Journal of Microbiology
Autores: Pansuriya,Ruchir C.; Singhal,Rekha S.
Lovastatin, an inhibitor of HMG-CoA reductase, was produced by solid state fermentation (SSF) using a strain of Aspergillus terreus UV 1718. Different solid substrates and various combinations thereof were evaluated for lovastatin production. Wheat bran supported the maximum production (1458 ± 46 µg g-1 DFM) of lovastatin. Response surface methodology (RSM) was applied to optimize the medium constituents. A 2(4) full-factorial central composite design (CCD) was chosen to explain the combined effects of the four medium constituents, viz. moisture content, particle size of the substrate, di-potassium hydrogen phosphate and trace ion solution concentration. Maximum lovastatin production of 2969 µg g-1 DFM was predicted by the quadratic model which was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lovastatin; Response surface methodology; Solid state fermentation; Aspergillus terreus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100024

Physiological studies on carboxymethyl cellulase formation by Aspergillus terreus DSM 826
Provedor de dados: Brazilian Journal of Microbiology
Autores: Abdel-Fatah,Osama M.; Hassan,Mohamed M.; Elshafei,Ali M.; Haroun,Bakry M.; Atta,Housam M.; Othman,Abdelmageed M.
Physiological studies were conducted to determine the optimum cultural conditions for maximal carboxymethyl cellulase (CMCase) formation by Aspergillus terreus DSM 826. Shaking condition at 150 rpm is favorable for the production of CMCase from rice straw and sugar cane bagasse. The highest enzyme yield was obtained at the third day of incubation at 30ºC for both cases; however CMCase formation occurred at a broad range of pH values, with maximal formation of A. terreus DSM 826 CMCase at pH 4.5 and 5.0 when rice straw and sugar cane bagasse were used as sole carbon source, respectively. Carboxymethyl cellulose (CMC) was found to be a good inducer for CMCase formation in both agricultural wastes with CMC concentrations of 0.5 and 1.0 % (w/v) in case of rice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus terreus; Carboxymethyl cellulase (CMCase); Rice straw; Sugar cane bagasse.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100001

Statistical optimization of kojic acid production by a UV-induced mutant strain of Aspergillus terreus
Provedor de dados: Brazilian Journal of Microbiology
Autores: Shakibaie,Mojtaba; Ameri,Atefeh; Ghazanfarian,Roya; Adeli-Sardou,Mahboubeh; Amirpour-Rostami,Sahar; Torkzadeh-Mahani,Masoud; Imani,Mehdi; Forootanfar,Hamid.
ABSTRACT The ability of four Aspergillus strains for biosynthesis of kojic acid was evaluated among which Aspergillus terreus represented the highest level (2.21 g/L) of kojic acid production. Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5–40 min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10, 7.63 g/L). Thereafter, design of experiment protocol was employed to find medium components (glucose, yeast extract, KH2PO4 (NH4)2SO4, and pH) influences on kojic acid production by the C5-10 mutant. A 25-1 fractional factorial design augmented to central composite design showed that glucose, yeast extract, and KH2PO4 were the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kojic acid; Aspergillus terreus; UV mutagenesis; Medium optimization.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400865

Enhanced alkaline cellulases production by the thermohalophilic Aspergillus terreus AUMC 10138 mutated by physical and chemical mutagens using corn stover as substrate
Provedor de dados: Brazilian Journal of Microbiology
Autores: Isaac,George Saad; Abu-Tahon,Medhat Ahmed.
Abstract A thermohalophilic fungus, Aspergillus terreus AUMC 10138, isolated from the Wadi El-Natrun soda lakes in northern Egypt was exposed successively to gamma and UV-radiation (physical mutagens) and ethyl methan-sulfonate (EMS; chemical mutagen) to enhance alkaline cellulase production under solid state fermentation (SSF) conditions. The effects of different carbon sources, initial moisture, incubation temperature, initial pH, incubation period, inoculum levels and different concentrations of NaCl on production of alkaline filter paper activity (FPase), carboxymethyl cellulase (CMCase) and β-glucosidase by the wild-type and mutant strains of A. terreus were evaluated under SSF. The optimum conditions for maximum production of FPase, CMCase and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thermohalophilic fungus; Aspergillus terreus; Alkaline cellulase; Solid state fermentation; Corn stover.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401269