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Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
Provedor de dados: 5
Autores: Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: Text
Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf