Consulta: subjectFacets:"Aujeszky's disease virus"
Registros recuperados: 3
Data/hora: 02/06/2024 09:36:06

Development of DNA probe for detection of Aujeszky's disease virus
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Cândido,A.L.; Barros,P.P.; Resende,M.; Flaschart,R.B.
A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aujeszky's disease virus; Dot-blot hybridization; Radioactive probe; Non radioactive probe.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001

Characterization of Aujeszky's disease virus isolates from South and Southeast Brazil by RFLP analysis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Piatti,Rosa Maria; Ikuno,Alice Akimi; Cunha,Elenice Sequetin; D'Ambros,Régia; Gregori,Fábio; Soares,Rodrigo Martins; Cortez,Adriana; Richtzenhain,Leonardo José.
The genomic DNA of thirty strains of Aujeszky's disease virus (ADV) isolated in the South and Southeast regions of Brazil from 1982 to 1996 were characterized by restriction endonuclease analysis with BamHI. Twenty seven strains were isolated from pigs, 1 from cattle, 1 from cat and 1 from dog. Using a systematization previously described, the 30 ADV strains could be classified as genomic types I (n = 2) and II (n = 28). Genomic type III was not observed. In this first study of genomic type characterization of brazilian ADV strains, we could demonstrate the occurence in Brazil of the genomic types I and II, with a large predominance of genomic type II.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aujeszky's disease virus; Swine; Genomic RFLP; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200015

Caracterization of Aujeszky's disease virus isolated from South Brazil in the last twenty years by restriction enzyme analysis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Schaefer,Rejane; Zanella,Janice Ciacci; Mores,Nelson; Pan,Kleitton Adolfo; Dambros,Régia Maria Feltrim; Schiochet,Marisete Fracasso; Coldebella,Michele.
Aujeszky's disease virus (ADV) belongs to Herpesviridae family and is an important etiological agent which infects pigs causing economic losses in swine producing countries worldwide and international trade restrictions to products of swine origin. An eradication program for ADV was established in Santa Catarina State since 2001. The last outbreak was reported in July 2004 and since then none has been reported. The disease has been controlled with the use of a genetic modified vaccine and elimination of seropositives. Aiming the characterization of ADV isolated in the South of Brazil in the last twenty years (1983-2003), a retrospective study based on the genomic analysis of the isolates through a digestion of viral genomic DNA with restriction enzyme Bam...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aujeszky's disease virus; Swine; Restriction endonuclease analysis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300035