Consulta: subjectFacets:"B-galactosidase"
Registros recuperados: 4
Data/hora: 05/06/2024 06:39:54

Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
Provedor de dados: Electronic Journal of Biotechnology
Autores: Dagbagli,Seval; Goksungur,Yekta.
This paper investigates the production and optimization of b-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four fermentation parameters (agitation speed, pH, initial substrate concentration and incubation time) on b-galactosidase enzyme production. Results of the statistical analysis showed that the fit of the model was good in all cases. Maximum specific enzyme activity of 4218.4 U g-1 was obtained at the optimum levels of process variables (pH 7.35, agitation speed 179.2 rpm,...
Tipo: Journal article
Palavras-chave: B-galactosidase; Kluyveromyces lactis; Response surface methodology; Shake flask culture; Synthetic medium.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400011

Mise au point de méthodes de manipulation embryonnaire de mollusques bivalves. Application en génétique et pathologie infectieuse
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cadoret, Jean-paul.
Après vingt ans de programmes pivilégiant les méthodes d'élevage, les recherches concernant l'aquaculture, évoluent maintenant vers les domaines de la pathologie infectieuse et la génétique. La maîtrise des productions larvaires a en effet favorisé l'étude de la reproduction et de l'embryologie, et permet désormais d'aborder des travaux expérimentaux sur les différents stades embryonnaires. La polyploïdisation d'oeufs de moules ou d'huîtres a d'abord été envisagée par l'application d'un procédé d'électrofusion. Des expérimentations à grande échelle ont permis l'obtention d'embryons triploïdes (55% chez les huîtres et 36% chez les moules) ainsi que tétraploïdes (20% chez les huîtres et 26% chez les moules). La microinjection de spermatozoïdes a permis de...
Tipo: Text
Palavras-chave: Mollusques; Bivalves; Polyploïdie; Electrofusion; Microinjection; Fécondation assistée; Rickettsies; Embryoculture; Trasfert de gène; B-galactosidase; Résistance; Pathologie; Génétique.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00440/55127/56585.pdf

Avaliação da atividade das enzimas pectina metilesterase e β-Galactosidase em mamões cv. Golden armazenados sob diferentes concentrações de oxigênio
Provedor de dados: Revista Brasileira de Fruticultura
Autores: Pinto,Luciana Konda de Azevedo; Martins,Meire Lelis Leal; Resende,Eder Dutra de; Thièbaut,José Tarcísio Lima; Martins,Marco Antonio.
Este trabalho foi realizado com o objetivo de avaliar o efeito de atmosferas controladas contendo diferentes concentrações de oxigênio sobre a atividade das enzimas β-galactosidase e pectina metilesterase, e sobre a cor da casca e a firmeza da polpa de mamões 'Golden'. Os frutos foram mantidos por 36 dias, nas seguintes atmosferas controladas: 1% de O2 e 0,03% CO2 com adsorvedor de etileno, 3% de O2 e 0,03% de CO2 com adsorvedor de etileno, 5% O2 e 0,03% de CO2 com adsorvedor de etileno e atmosfera ambiente sem adsorvedor de etileno. A UR e a temperatura foram mantidas entre 85-95% e a 13º C, respectivamente. Os frutos estocados sob atmosfera de 1% de O2 e 0,03% CO2 apresentaram retardamento nas atividades das enzimas β-galactosidase e pectina...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carica papaya; Atmosfera controlada; B-galactosidase; Pectinametilesterase; Firmeza de polpa.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000100003

Purification of microbial b-galactosidase from Kluyveromyces fragilis by bioaffinity partitioning
Provedor de dados: Revista de Microbiologia
Autores: Silva,Maria Estela da; Franco,Telma Teixeira.
This work investigated the partitioning of b-galactosidase from Kluyveromyces fragilis in aqueous two-phase systems (ATPS) by bioaffinity. PEG 4000 was chemically activated with thresyl chloride, and the biospecific ligand p-aminophenyl 1-thio-b-D-galactopyranoside (APGP) was attached to the activated PEG 4000. A new two-step method for extraction and purification of the enzyme b-galactosidase from Kluyveromyces fragilis was developed. In the first step, a system composed of 6% PEG 4000-APGP and 8% dextran 505 was used, where b-galactosidase was strongly partitioned to the top phase (K = 2,330). In the second step, a system formed of 13% PEG-APGP and 9% phosphate salt was used to revert the value of the partition coefficient of b-galactosidase (K = 2 x...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B-galactosidase; Aqueous two-phase systems; Protein purification; Downstream-processing; Affinity.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400006