Consulta: subjectFacets:"Bacteria"
Registros recuperados: 341
Data/hora: 03/06/2024 08:19:22

Estados fenológicos tempranos de frijol [Vigna unguiculata (L.) Walp], bacterias y actividad enzimática: indicadores de toxicidad del petróleo intemperizado.
Provedor de dados: 32
Autores: Ramírez May, Ana Guadalupe.
El objetivo de esta investigación fue evaluar el efecto de los hidrocarburos totales del petróleo intemperizado (HTPI) al final de dos estados del crecimiento vegetal, en la densidad de bacterias fijadoras de N de vida libre (BFN) en suelo-rizosfera, de asociación simbiótica (Rhizobium) en nódulos y la actividad enzimática de la ureasa en suelo plantado con Vigna unguiculata, para obtener indicadores biológicos de toxicidad. Se utilizaron dos Gleysoles del estado de Tabasco, México. Gleysol 1 (sitio 1) ubicado a 2 Km al sureste del Complejo Procesador de Gas La Venta, en el ejido José Narciso Rovirosa, Huimanguillo, se colectaron en nueve puntos suelos con diferentes contenidos de HTPI (5 822, 6 536, 13 803, 14 431, 15 923, 23 313, 25 517, 28 465, 50 297...
Palavras-chave: Bacterias; Nódulos; Hidrocarburos; Toxicidad; Bacteria; Nodules; Hydrocarbons; Toxicity; Producción Agroalimentaria en el Trópico; Maestría.
Ano: 2012
URL: http://hdl.handle.net/10521/716

Estudio comparativo de tres épocas del año en características químicas, plantas y organismos de suelo contaminado con petróleo en La Venta, Tabasco, México.
Provedor de dados: 32
Autores: Dorantes Avelino, Rodrigo.
En esta investigación el objetivo fue evaluar las propiedades físicas, químicas y biológicas en un suelo Agrícola y Pecuario para un día específico en tres épocas del año, así como el efecto de los hidrocarburos del petróleo y acumulación de precipitación en el ejido José Narciso Rovirosa, Huimanguillo, Tabasco. El sitio seleccionado es un área de 44,321.199 m2 ubicada al sureste del Complejo Procesador de Gas La Venta, el suelo es receptor de petróleo crudo desde hace 20 años y fue descontaminado en abril 2002. Se establecieron (de agosto 2009  a abril 2010) nueve puntos (2x2 m) experimentales distribuidos completamente al azar. Se realizaron tres muestreos (30 de septiembre del 2009, 30 de enero y 30 de abril del 2010). Se determinó; la acumulación de...
Palavras-chave: Bacterias; Biomasa aérea; Hongos; Hidrocarburos; Precipitación; Bacteria; Biomass; Fungi; Hydrocarbons; Precipitation; Maestría; Producción Agroalimentaria en el Trópico.
Ano: 2010
URL: http://hdl.handle.net/10521/240

Estudio comparativo de tres épocas del año en características químicas, plantas y organismos de suelo contaminado con petróleo en La Venta, Tabasco, México.
Provedor de dados: 32
Autores: Dorantes Avelino, Rodrigo.
En esta investigación el objetivo fue evaluar las propiedades físicas, químicas y biológicas en un suelo Agrícola y Pecuario para un día específico en tres épocas del año, así como el efecto de los hidrocarburos del petróleo y acumulación de precipitación en el ejido José Narciso Rovirosa, Huimanguillo, Tabasco. El sitio seleccionado es un área de 44,321.199 m2 ubicada al sureste del Complejo Procesador de Gas La Venta, el suelo es receptor de petróleo crudo desde hace 20 años y fue descontaminado en abril 2002. Se establecieron (de agosto 2009  a abril 2010) nueve puntos (2x2 m) experimentales distribuidos completamente al azar. Se realizaron tres muestreos (30 de septiembre del 2009, 30 de enero y 30 de abril del 2010). Se determinó; la acumulación de...
Palavras-chave: Bacterias; Biomasa aérea; Hongos; Hidrocarburos; Precipitación; Bacteria; Biomass; Fungi; Hydrocarbons; Precipitation; Maestría; Producción Agroalimentaria en el Trópico.
Ano: 2010
URL: http://hdl.handle.net/10521/240

Aislamiento y caracterización bioquímica de metabolitos producidos por rizobacterias que solubilizan fosfato.
Provedor de dados: 32
Autores: Paredes Mendoza, Marianela.
El empleo de bacterias solubilizadoras de fosfato (BSP) para la solubilización de distintas rocas fosfatadas y de otras fuentes de fósforo es una alternativa para incrementar la cantidad de P disponible para las plantas. Se aislaron 147 BSP de la raíz y rizoplano  de diferentes  plantas cultivadas.  El  medio de cultivo  denominado Medio Mínimo para Solubilización de Fosfato Colegio de Postgraduados con MgCl (MMSFCP-Mg) fue el más eficiente,  debido a que en medio sólido se observaron halos de solubilización de hasta 3.8 cm a los  catorce días, y en medio líquido se determinaron hasta   380 mg mL-1  de fosfato soluble a los seis días.   Veintisiete  de las mejores cepas     fueron seleccionadas  con base  en la eficiencia de solubilización de fosfato...
Palavras-chave: Acidos orgánicos; Fosfatasas; Solubilización de fosfato; Inmunodetección; Roca fosfórica; Bacterias solubilizadoras de fosfato; Enfófitas; Organic acids; Phosphatases; Phosphate solubilization; Immunodetection; Rock phosphate; Phosphate solubilizing; Bacteria; Endophyte; Doctorado; Edafología.
Ano: 2010
URL: http://hdl.handle.net/10521/245

Biodeterioration patterns found in dammar resin used as art material
Provedor de dados: 69
Autores: Romero-Noguera,Julio; Martín-Sánchez,Inés; López-Miras,María del Mar; Ramos-López,José Miguel; Bolívar-Galiano,Fernando.
Since the middle of the XIX century, when dammar became popular in Occident, this natural resin is one of the most used in art painting techniques as final protective coating (varnish) as well as a component of pictorial media. The present work is the first approach to the study of the microbiological biodeterioration of this artistic material, which can seriously affect the appearance and integrity of works of art when bad conservation conditions -especially high humidity levels- take place. 12 microorganisms, fungi and bacteria, came from collection and from oil paintings affected by biodeterioration patterns, were inoculated on test specimens prepared with varnish dammar. These were incubated and analyzed by GC-MS to determine both the microbiological...
Tipo: Journal article
Palavras-chave: Bacteria; Biodeterioration; Dammar; Fungi; GC-MS.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300006

Bionanoparticles, a green nanochemistry approach
Provedor de dados: 69
Autores: Cauerhff,Ana; Castro,Guillermo R.
Background: In the past decade, considerable attention has been paid for the development of novel strategies for the synthesis of different kind of nano-objects. Most of the current strategies are usually working by the use physical or chemical principles to develop a myriad of nano-objects with multiple applications. Main fields of nanotechnology applications range from catalysis, micro- and nano-electronics (semiconductors, single electrons transistors), non-linear optic devices, photo-electrochemistry to biomedicine, diagnostics, foods and environment, chemical analysis and others. Results: Two main avenues for nanoparticles synthesis: cell-free extract and cell cultivation have been reported. The state of art of both biotechnological approaches for...
Tipo: Journal article
Palavras-chave: Bacteria; Bionanoparticles; Fermentation; Fungi green chemistry; Plant extracts.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300011

A microbial community analysis of the octocoral Eunicea fusca
Provedor de dados: 69
Autores: Duque-Alarcón,Angela; Santiago-Vázquez,Lory Z; Kerr,Russell G.
While there is a significant and growing body of knowledge describing the microbial communities of marine invertebrates such as sponges, there are very few such studies focused on octocorals. The octocoral Eunicea fusca is common on reefs in various regions of the Caribbean and has been the subject of natural product investigations. As part of an effort to describe the microbial community associated with octocorals, a culture-independent analysis of the bacterial community of E. fusca was conducted. Specifically, a 16S rDNA clone library analysis was performed to provide baseline data. A total of 40 bacteria members from 11 groups were found. In general, Proteobacteria were the dominant group with a total of 24 species and &amp;#945;-Proteobacteria...
Tipo: Journal article
Palavras-chave: 16S rDNA; Bacteria; Culture-independent analysis; Eunicea fusca; Microbial community; Octocoral.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500015

Ethanol mediated enhancement in bacterial transformation
Provedor de dados: 69
Autores: Sharma,Arun Dev; Singh,Jaspreet; Gill,Prabhjot Kaur.
In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.
Tipo: Journal article
Palavras-chave: Bacteria; Ethanol; Transformation.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016

Comparison of commercially-available RNA extraction methods for effective bacterial RNA isolation from milk spiked samples
Provedor de dados: 69
Autores: García-Nogales,Paula; Serrano,Alicia; Secchi,Sonia; Gutiérrez,Serafín; Arís,Anna.
Nucleic-acid based methods for bacterial identification are extremely useful in diagnostic applications due to their specificity and sensitivity. However, they require an optimal purification of the target molecules. As part of the development of a new diagnostic method for the detection of bacterial RNA in cow milk, we have compared four commercially available RNA extraction kits for the isolation of bacterial RNA from spiked UHT milk samples. The kits were compared in terms of extraction efficiency and RNA purity using two bacterial species, the Gram negative Escherichia coli and the Gram positive Staphylococcus aureus. Two kits are based in silica-matrix extraction, and the other two in the guanidinium thiocyanate-phenol-chloroform extraction. In our...
Tipo: Journal article
Palavras-chave: Bacteria; Milk; Purification; RNA.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500019

Silanol - A novel class of antimicrobial agent
Provedor de dados: 69
Autores: Kim,Yun-mi; Farrah,Samuel; Baney,Ronald H.
Recently, a significant amount of attention has been directed toward development of novel classes of biocides because of the potential for microbial contamination and infection risks to military personnel and in the general population. We have recently discovered a new and unexpected class of powerful biocides based upon compounds derived through simple chemistry from silicone intermediates, "silicon alcohols" called, silanols. One example is trialkylsilanols, R3SiOH. Antimicrobial tests were carried out against Gram-negative bacterium, Escherichia coli, and Gram-positive, Staphylococcus aureus, with silanols, t-butanol, and siloxanes. Trialkylsilanols were very effective biocides against Escherichia coli and Staphylococcus aureus. The number of viable...
Tipo: Journal article
Palavras-chave: Antimicrobial agents; Bacteria; Biocide; Silanols.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200012

Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria
Provedor de dados: 69
Autores: Odisi,Estácio Jussie; Silvestrin,Marcela Bruschi; Takahashi,Rodrigo Yoji Uwamori; da Silva,Marcus Adonai Castro; Lima,André Oliveira de Souza.
Background: Cellulases and lipases have broad industrial application, which calls for an urgent exploration of microorganisms from extreme environments as valuable source of commercial enzyme. In this context, the present work describes the bioprospection and identification of deep-sea bacteria that produce cellulases and lipases, as well their optimal temperature of activity. Results: The first step of this study was the screening of cellulolytic and lipolytic deep-sea bacteria from sediment and water column, which was conducted with substrates linked with 4-Methylumbelliferyl. Among the 161 strains evaluated, 40 were cellulolytic, 23 were lipolytic and 5 exhibited both activities. Cellulolytic and lipolytic bacteria are more common in sediment than at...
Tipo: Journal article
Palavras-chave: Bacteria; Cellulases; Deep-sea; Lipases.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500018

Novel antimicrobial activity of a dichloromethane extract obtained from red seaweed Ceramium rubrum (Hudson) (Rhodophyta: Florideophyceae) against Yersinia ruckeri and Saprolegnia parasitica, agents that cause diseases in salmonids
Provedor de dados: 69
Autores: Cortés,Yurima; Hormazábal,Emilio; Leal,Hellmuth; Urzúa,Alejandro; Mutis,Ana; Parra,Leonardo; Quiroz,Andrés.
Background Enteric red mouth disease and Saprolegniasis, which are caused by the bacteria Yersinia ruckeri and the oomycete Saprolegnia parasitica, respectively, are important illnesses that affect salmonid farming. Sanitary problems in farms are addressed by the prevention of disease outbreaks or by the treatment of diseases with chemicals. Environmental and governmental restrictions, toxicity and high treatment costs limit the use of drugs. Marine organisms, such as algae, sponges and corals, have developed an antimicrobial defense strategy based on the production of bioactive metabolites. Among these organisms, seaweeds offer a particularly rich source of potential new drugs. Hence, many pharmacologically active substances have been isolated from...
Tipo: Journal article
Palavras-chave: Antimicrobial activity; Bacteria; Fish pathogens; Lipophilic extract.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300004

Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers
Provedor de dados: 69
Autores: Brandelli,Adriano; Riffel,Alessandro.
The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37ºC, with maximum activity and yield at 30ºC. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30ºC and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic...
Tipo: Journal article
Palavras-chave: Bacteria; Enzyme; Keratin; Protease.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100007

Leaf litter decomposition from native and non-native species in a freshwater forested wetland of Chile
Provedor de dados: 72
Autores: Gómez-Capponi,Francisco; Correa-Araneda,Francisco; Díaz,Ma Elisa; Olguín,Manuel; Encina-Montoya,Francisco; Figueroa,Ricardo.
ABSTRACT Decomposition of leaf litter is a fundamental process for the functioning of forested wetlands. The rapid increment of forest plantations has involved a greater contribution of leaf litter of exotic origin to these ecosystems. The decomposition rate between leaf litter of native and exotic origin in a forested wetland of the Mediterranean zone of Chile was compared, as well as the contribution of bacteria and macroinvertebrate to this process. It was determined that the decomposition rate of the leaf litter presented significant differences (p &lt; 0.05) between species, being faster in those of non-native origin. This could be due to differences in ecophysiological aspects of the leaves like the presence of chemical compounds such as waxes or...
Tipo: Journal article
Palavras-chave: Allochthonous detritus; Bacteria; Lentic ecosystem; Macroinvertebrates.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382017000100001

Leaf-associated bacterial microbiota of coffee and its correlation with manganese and calcium levels on leaves
Provedor de dados: 74
Autores: Sousa,Leandro Pio de; Silva,Marcio José da; Costa Mondego,Jorge Maurício.
Abstract Coffee is one of the most valuable agricultural commodities and the plants’ leaves are the primary site of infection for most coffee diseases, such as the devastating coffee leaf rust. Therefore, the use of bacterial microbiota that inhabits coffee leaves to fight infections could be an alternative agricultural method to protect against coffee diseases. Here, we report the leaf-associated bacteria in three coffee genotypes over the course of a year, with the aim to determine the diversity of bacterial microbiota. The results indicate a prevalence of Enterobacteriales in Coffea canephora, Pseudomonadales in C. arabica ‘Obatã’, and an intriguing lack of bacterial dominance in C. arabica ‘Catuaí’. Using PERMANOVA analyses, we assessed the association...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Bacteria; 16S; Leaf; Manganese; Calcium.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300455

Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
Provedor de dados: 74
Autores: Rudi Emerson de Lima,Procópio; Araújo,Welington Luiz; Andreote,Fernando Dini; Azevedo,João Lúcio.
A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Green fluorescent protein; Bacteria; Host plant; Eucalyptus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100018

Wolbachia in guilds of Anastrepha fruit flies (Tephritidae) and parasitoid wasps (Braconidae)
Provedor de dados: 74
Autores: Mascarenhas,Rodrigo O; Prezotto,Leandro F; Perondini,André Luiz P; Marino,Celso Luiz; Selivon,Denise.
Abstract The endosymbiont Wolbachia is efficiently transmitted from females to their progenies, but horizontal transmission between different taxa is also known to occur. Aiming to determine if horizontal transmission might have occurred between Anastrepha fruit flies and associated braconid wasps, infection by Wolbachia was screened by amplification of a fragment of the wsp gene. Eight species of the genus Anastrepha were analyzed, from which six species of associated parasitoid wasps were recovered. The endosymbiont was found in seven Anastrepha species and in five species of braconids. The WSP Typing methodology detected eight wsp alleles belonging to Wolbachia supergroup A. Three were already known and five were new ones, among which four were found to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Fruit flies; Horizontal transmission; Wsp gene; Recombination.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400600

Санитарно-биологические показатели морской воды бухты Артиллерийской Чёрного моря.
Provedor de dados: 13
Autores: Миронов, О. Г.; Муравьёва, И. П.; Миронова, Т. О.
Выполнен комплексный санитарно-биологический анализ морской воды (липидно-углеводородный комплекс, численность гетеротрофных и нефтеокисляющих бактерий, диатомовых и пирофитовых водорослей) в одной из бухт (Артиллерийская) в районе Севастополя (Чёрное море). Количество нефтяных углеводородов в воде на протяжении исследованного периода в 79 % случаев превышало ПДК в среднем в 1.5 – 2 раза;  средняя концентрация липидов и углеводородов составляла соответственно 0.14 и 0.11 мг/л. Наблюдается высокая степень корреляционной связи между содержанием липидов и углеводородов (R= 0.86). Отмечена тенденция повышения концентрации липидов в морской воде с увеличением численности диатомовых водорослей. Коэффициент корреляции между этими величинами составил R= 0.62.
Tipo: Journal Contribution
Palavras-chave: Липидно-углеводородный комплекс; Нефтяные углеводороды; Бактерии; Микроводоросли; Севастополь; Чёрное море; Lipid-hydrocarbon complex; Oil-hydrocarbon; Bacteria; Microalga; Sevastopol; Black Sea; Ліпідно-вуглеводневий комплекс; Нафтові вуглеводні; Бактерії; Мікроводорості; Чорне море.
Ano: 2008
URL: http://repository.ibss.org.ua/dspace/handle/99011/812

Экспериментальное изучение развития тионовых и денитрифицирующих бактерий в анаэробных и  аэробных условиях
Provedor de dados: 13
Autores: Бурдиян, Н. В.
Изучено изменение численности тионовых и денитрифицирующих бактерий, выделенных из прибрежных наносов Севастопольских бухт, в экспериментально созданных  аэробных и анаэробных условиях. Отмечено возрастание численности тиобактерий при росте в анаэробных условиях и интенсивное развитие денитрификаторов,  как в аэробных, так и анаэробных условиях.
Tipo: Journal Contribution
Palavras-chave: Abundance; Bacteria; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2007
URL: http://repository.ibss.org.ua/dspace/handle/99011/579

Alteromonas -подобные бактерии, выделенные из воды и беспозвоночных Черного моря, и их антагонистическая активность.
Provedor de dados: 13
Autores: Онищенко, О. М.
51 штамм Alteromonas-подобных бактерий, изолированных из воды и беспозвоночных Черного моря, а также 11 типовых штаммов родов Pseudoalteromonas, Alteromonas, Marinomonas, Shewanella исследованы по 97 фенотипическим признакам и сгруппированы в 8 фенонов методами нумерического анализа. Черноморские изоляты отнесены к видам Pseudoalteromonas nigrifaciens, P. citrea, P. aurantia, P. haloplanktis, P. undina. Спектр их антагонистической активности отличался на различных по составу питательных средах: на богатой среде "В" антагонизм наблюдался в отношении спороносных бактерий и Candida albicans, на синтетической среде "ВМ" - в отношении P. aeruginosa, и в меньшей степени - дрожжей рода Candida.
Tipo: Journal Contribution
Palavras-chave: Bacteria; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2001
URL: http://repository.ibss.org.ua/dspace/handle/99011/561

Сульфатредуцирующая группа бактерий в прибрежных наносах бухты Круглой (Севастополь. Чёрное море)
Provedor de dados: 13
Autores: Бурдиян, Н. В.
Исследовано распространение и численность сульфатредуцирующей группы бактерий в прибрежных наносах бухты Круглой (Севастополь, Чёрное море). Отмечена высокая вариабельность полученных данных: от 0.4 до 9.5-103 кл./г. Сезонная динамика численности бактерий не выявлена.
Tipo: Journal Contribution
Palavras-chave: Bacteria; Seasonal distribution; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2007
URL: http://repository.ibss.org.ua/dspace/handle/99011/602

Diagnóstico de Ralstonia solanacearum em tomateiro.
Provedor de dados: 14
Autores: LOPES, C. A.; ROSSATO, M.
Pretende-se, com esta publicação, fornecer informações sobre os processos envolvidos na identificação da doença, coleta e envio de material para análise laboratorial, técnicas de identificação e armazenamento de cepas do patógeno.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Murcha-bacteriana.; Tomate; Doença de planta; Bacteria; Ralstonia Solanacearum.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/960772

Prevenir o pior.
Provedor de dados: 14
Autores: OLIVEIRA, E. de; VALICENTE, F. H.
bitstream/item/73789/1/Previnir-pior.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Doença de planta; Mancha foliar; Bacillus thuringiensis; Bacteria.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/488623

Levantamento de doenças e mosca-branca em tomateiro em regiões produtoras no Brasil.
Provedor de dados: 14
Autores: QUEZADO-DUVAL, A. M.; INOUE-NAGATA, A. K.; REIS, A.; PINHEIRO, J. B.; LOPES, C. A.; ARAÚJO, E. R.; FONTENELLE, M. R.; COSTA, J. R.; GUIMARÃES, C. M. N.; ROSSATO, M.; BECKER, W. F.; COSTA, H.; FERREIRA, M. A. S. V.; DESTÁFANO, S. A. L.
No intuito de estudar quais as principais doenças para os cultivos nacionais, realizou-se um levantamento no período de 2008 a 2011 em diferentes regiões produtoras no Brasil, que abrangeu 93 lavouvas de tomate para mesa em 12 estados e no Distrito Federal e 51 lavouras nos três estados produtores de tomate para processamento industrial e no Distrito Federal.
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Tomate; Doença de planta; Praga; Inseto; Mosca branca; Virus; Bacteria; Fungo; Nematoide.; Solanum lycopersicum.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/974139

Manual de identificação de doenças de soja.
Provedor de dados: 14
Autores: HENNING, A. A.; ALMEIDA, A. M. R.; GODOY, C. V.; SEIXAS, C. D. S.; YORINORI, J. T.; COSTAMILAN, L. M.; FERREIRA, L. P.; MEYER, M. C.; SOARES, R. M.; DIAS, W. P.
Doenças causadas por fungos. Doenças causas por bactérias. Doenças causadas por vírus. Doenças causadas por nematóides. Estádios de desenvolvimento da soja.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Doenças da soja.; Soja; Doença de planta; Fungo; Bacteria; Vírus; Nematóide; Glycine Max.; Soybeans; Plant diseases and disorders.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/577927

Micro-organismos.
Provedor de dados: 14
Autores: FERNANDES JUNIOR, P. I.
Pesquisas em relação à diversidade de plantas e animais tem demonstrado que a riqueza de micro-organismos nos solos na região semiárida do Nordeste é relativamente alta. Cada espécie de fungo e bactéria tem preferência por um conjunto de características de solo e clima, e como o Semiárido tem grande variedade de ambientes, essa variedade se traduz em um alto número de espécies de micro-organimos habitando os solos das diferentes regiões.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Bactérias fixadoras de nitorgênio; FMA; Fungos micorrízicos arbusculares.; Solo; Fungo; Bacteria; Inoculação; Microrganismo.; Soil.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1046759

Duplex PCR para a amplificação simultânea de fragmentos dos genes H e C em bactérias isoladas de nódulos de leguminosas.
Provedor de dados: 14
Autores: FERNANDES JUNIOR, P. I.; MORGANTE, C. V.; GAVA, C. A. T.; SANTOS, C. A. F.; CUNHA, J. B. A.; MARTINS, L. M. V.
bitstream/item/98607/1/COT158.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Fixação biológica do nitrogênio; FBN; Leguminosa; Bacteria.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/981647

Guia de identificacao das doencas do tomateiro.
Provedor de dados: 14
Autores: LOPES, C. A.
Requeima; Pinta-preta; Septoriose; Mancha-de-estenfilio; Oidio; Podridao-de-esclerotinia; Rizoctoniose; Vira-cabeca; Mosaico-dourado; Murcha-bacteriana; Cancro-bacteriano; Pinta-bacteriana; Mancha-bacteriana; Talo-oco ou podridao-mole; Galhas ou nematoide.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Oidiopsis sicula; Clavibacter michiganensis michiganensis; Pseudomonas syringae tomato; Xanthomonas campestris vesicatoria.; Tomate; Doença de planta; Fungo; Bacteria; Virus; Nematoide; Alternaria solani; Septoria lycopersici; Sclerotinia Sclerotiorum; Rhizoctonia Solani; Ralstonia Solanacearum; Meloidogyne spp; Phytophthora Infestans.; Tomatoes; Erysiphe; Nematoda; Solanum lycopersicum.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/768979

Ecosystemes benthiques profonds et chimiosynthese bacterienne: Sources hydrothermales et suintements
Provedor de dados: 5
Autores: Laubier, Lucien.
Hydrothermal communities of various species compositions have been described from the eastern and western Pacific and Atlantic. Brine and cold seeps and hydrocarbon seepages have been found off Florida, off the Oregon and Japanese subduction systems, on the Laurentian Fan, near Barbados, and off Louisiana. Biomasses range from 10 to 70 kg/m super(2), fresh weight. Basic microdistribution of species groups in aureoles centered around hydrothermal vents reveals different levels of adaptation to harsh physico-chemical conditions. Two groups of primary producers can be recognized: a highly efficient cool water group with large vestimentiferan tube worms and bivalves, and alvinellid polychaetes adapted to hot waters. Both groups are exploited by specific...
Tipo: Text
Palavras-chave: Bivalvia; Polychaeta; Bacteria; Symbionts; Biosynthesis; Chemosynthesis; Food webs; Biomass; Hydrocarbons; Ecosystems; Hydrothermal springs; Seepages; Brines; Benthic environment; Benthos.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/acte-1171.pdf

Bacterial Dynamics in a Microphytobenthic Biofilm: a Tidal Mesocosm Approach
Provedor de dados: 5
Autores: Agogue, Helene; Mallet, Clarisse; Orvain, Francis; De Crignis, Margot; Mornet, Francoise; Dupuy, Christine.
In intertidal mudflats, during low tide exposure, microphytobenthos (MPB) migrate vertically through the surface sediment and form, with the heterotrophic bacteria, a transient biofilm. Inside this biofilm, multiple interactions exist between MPB and bacteria. These micro-organisms secrete a wide range of extracellular polymeric substances (EPS), which are major components of the biofilm matrix. In this study, we used a tidal mesocosm experiment in order to decipher the interactions of the MPB-EPS-bacteria complex within the biofilm. We tried to determine if the EPS could control bacterial activities and/or production and/or richness according to the age of the biofilm and to the immersion/emersion period. The dynamics of biomasses of MPB and prokaryotes,...
Tipo: Text
Palavras-chave: Muddy sediment; Microphytobenthos; Bacteria; Extracellular polymeric substances; Interactions; Mesocosm.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00181/29271/27682.pdf

Eutrophication disrupts summer trophic links in an estuarine microbial food web
Provedor de dados: 5
Autores: López-abbate, M.c.; Molinero, Juan-carlos; Barría De Cao, M.s.; Silva, R.; Negri, R.; Guinder, V.a.; Hozbor, M.c.; Hoffmeyer, M.s.
Eutrophication is the most widespread effect of urban development in coastal areas. To elucidate how nutrient loading affects the carbon pathways at the base of food chains, we quantified the carbon transfer among microbial components, from prokaryotes to microzooplankton. For this purpose, we performed two size-fractionation feeding experiments during late summer under moderate (N: 28.5 μM, P: 0.92 μM, Si: 45.8 μM) and severe (N: 173.9 μM, P: 3.5 μM, Si: 67.2 μM) cultural eutrophication in the Bahía Blanca Estuary (Argentina). In both experiments, prokaryotes were largely dominated by heterotrophic forms, small diatoms dominated among autotrophs, and heterotrophic nanoflagellates were the most abundant among protistan grazers. Under severe eutrophication,...
Tipo: Text
Palavras-chave: Eutrophication; Microbial food web; Bacteria; Nanoflagellates; Microzooplankton; Estuaries.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00512/62335/66718.pdf

The dispersion of adhered marine bacteria by pyrophosphate and ultrasound prior to direct counting.
Provedor de dados: 5
Autores: Velji, M; Albright, L.
A technique has been investigated for dispersing adhered marine bacteria from various surfaces prior to direct counting. The bacterial cells were initially preserved and strengthened by use of 3.7% formaldehyde (v/v final concentration). Seawater samples were then treated with 0.001 M of tetrasodium pyrophosphate (final concentration), a sequestering and deflocculating agent. Surface sediment and kelp disc samples were treated with 0.01 M tetrasodium pyrophosphate (final concentration) after having been suspended in 0.44 M sodium chloride solution. These samples were incubated for 15-30 min. Subsequently, the samples were treated with ultrasound (100 W) for 30 to 45 s. This technique resulted in an even dispersion of most bacterial cells in the samples....
Tipo: Text
Palavras-chave: Bacteria; Microbiological analysis; Flocculation; Ultrasonics; Bacterial counters.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-968.pdf

Microflores sulfatoreductrices en milieu subantarctique. Relations avec quelques parametres physicochimiques et biologiques. (Microflores totales et heterotrophes)
Provedor de dados: 5
Autores: Delille, D; Bouvy, M.
The archipelago of Kerguelen with its complex fjord distribution is particularly interesting for the study of anoxic systems. Between 1968 and 1972, several stations were investigated fortnightly. One of these was further sampled weekly throughout 1982. Each sample series of sediment included the superficial aerobic and subsuperficial anaerobic levels. Each level was examined for direct epifluorescent counts, heterotrophic and sulfate-reducing bacterial numbers by M.P.N. methods, organic carbon and sulfide measurements. Sulfate-reducing bacteria seemed less related to total biomass than the heterotrophic microflora, the latter being dependent on organic supplies. The hydrogen sulfide production was directly related to the presence of sulfate reducers;...
Tipo: Text
Palavras-chave: Anoxic conditions; Bacteria; Heterotrophic organisms; Sulphur; Organic carbon; Physicochemical properties; Seasonal variations; Population number; Microorganisms; Polar waters; Sulfate reduction.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-970.pdf

Microbiota Composition and Evenness Predict Survival Rate of Oysters Confronted to Pacific Oyster Mortality Syndrome
Provedor de dados: 5
Autores: Clerissi, Camille; De Lorgeril, Julien; Petton, Bruno; Lucasson, Aude; Escoubas, Jean Michel; Gueguen, Yannick; Dégremont, Lionel; Mitta, Guillaume; Toulza, Eve.
Pacific Oyster Mortality Syndrome (POMS) affects Crassostrea gigas oysters worldwide and causes important economic losses. Disease dynamic was recently deciphered and revealed a multiple and progressive infection caused by the Ostreid herpesvirus OsHV-1 μVar, triggering an immunosuppression followed by microbiota destabilization and bacteraemia by opportunistic bacterial pathogens. However, it remains unknown if microbiota might participate to protect oysters against POMS, and if microbiota characteristics might be predictive of oyster mortalities. To tackle this issue, we transferred full-sib progenies of resistant and susceptible oyster families from hatchery to the field during a period in favor of POMS. After 5 days of transplantation, oysters from...
Tipo: Text
Palavras-chave: Holobiont; Microbiome; Metabarcoding; Fitness; Bacteria.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00621/73332/72500.pdf

Pathogens in aquaculture : specific pathogens or human contamination risks
Provedor de dados: 5
Autores: Renault, Tristan.
The vatness of aquaculture ventures worldwide has taken impressive leaps durint the past three decades, and a large portion of this pertains to mariculture. Significant strides have been made in the design and implementation of more efficient and cost effective growing areas, feed, and harvesting techniques. Moreover the economic impact of aquaculture will certainly increase in the years ahead.
Tipo: Text
Palavras-chave: Mycoplasmas; Chlamydiae; Rickettsia; Bacteria; Virus; Aquaculture; Pathogens.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2899.pdf

Mise en evidence et evaluation de l'enrichissement bacterien du film de surface des eaux littorales d'effluents urbains
Provedor de dados: 5
Autores: Beucher, M; Plusquellec, A; Legal, Y; Cleret, J.
Sea water samples were collected in the vicinity of urban non-treated, urban treated and industrial outfalls. The samples belonged to two differents types: the first one consisted of the surface film, the second was the corresponding water at 50 cm depth. The enumeration of the bacteria in both types of samples indicated a significant concentration of the mesophilic flora and of the indicators of fecal pollution in the surface film. The value of the concentrating factor depended largely on the nature of the sewage and did not reflect differences between the various bacterial groups. At one of the stations a very significant regression was demonstrated between surface sample and depth sample.
Tipo: Text
Palavras-chave: Bacteria; Bacteriology; Bacterial counters; Industrial wastes; Pollution indicators; Coastal waters; Microbial contamination; Indicator species; Sewage; Outfalls; Surface films.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1006.pdf

Contribution d'un film bacterien a la culture intensive de Daphnia magna
Provedor de dados: 5
Autores: Langis, R; Proulx, D; Lanoue De La, J; Couture, P.
Laboratory scale experiments show that can increase of the biofilm in cultivation tanks improves significantly the productivity of Daphnia
Tipo: Text
Palavras-chave: Daphnia magna; Branchiopoda; Bacteria; Detoxification; Nutrition; Interspecific relationships.
Ano: 1987
URL: http://archimer.ifremer.fr/doc/1987/acte-1411.pdf

Influence of environment factors on bacterial ingestion rate of the deposit-feeder Hydrobia ulvae and comparison with meiofauna
Provedor de dados: 5
Autores: Pascal, Pierre-yves; Dupuy, C; Richard, P; Haubois, A.g.; Niquil, N.
Deposit feeders are able to process a considerable volume of sediment, containing large quantities of associated bacteria. However, conclusions concerning the trophic role played by benthic bacteria in marine sediments are still not fully elucidated. This study deals with bacterivory by the gastropod Hydrobia ulvae, one of the most abundant deposit-feeding species in intertidal mudflats in Western Europe. Ingestion rates of bacteria were determined during grazing experiments using 15N preenriched bacteria. Grazing experiments were performed in order to measure effects of abiotic (temperature, salinity and luminosity) and biotic (bacterial and algal abundances) factors on ingestion rates of bacteria by H. ulvae of an intertidal mudflat (Brouage,...
Tipo: Text
Palavras-chave: Mudflat; Nematodes; Ammonia tepida; Environmental factors; Grazing; Bacteria; Gastropod; Hydrobia ulvae; Deposit feeding.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4781.pdf

Screening and selection of growth-promoting bacteria for Dunaliella cultures
Provedor de dados: 5
Autores: Le Chevanton, Myriam; Garnier, Matthieu; Bougaran, Gael; Schreiber, Nathalie; Lukomska, Ewa; Berard, Jean-baptiste; Fouilland, E.; Bernard, Olivier; Cadoret, Jean-paul.
Previous studies have demonstrated that bacteria influence microalgal metabolism, suggesting that the selection and characterization of growth-promoting bacteria should offer a new strategy for improving industrial algal cultivation. In the present study, 48 cultivable bacteria were isolated from marine microalgae species and identified using 16S rRNA phylogenetic analysis. The recovered bacteria were found to be members of the α- and γ-Proteobacteria, Cytophaga–Flavobacterium–Bacteroides (CFB) and gram-positive monophyletic clusters. To address the effect of these bacteria on the growth of Dunaliella sp. individually, an experimental high-throughput tool was developed to simultaneously compare replicated associations. A two-step approach was used to...
Tipo: Text
Palavras-chave: Microalgae; Dunaliella; Bacteria; Interaction; Bacterial diversity.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00142/25334/24768.pdf

Heavy metals effect on bacterial communities from coastal sediments.
Provedor de dados: 5
Autores: Modamio, X; Mallo, S.
The effects of heavy metals on bacterial communities of sea sediments are studied. Samples were taken from a very polluted zone near Barcelona. Bacterial populations were estimated by the plate count method, using culture medium amended with heavy metals (Hg, Cd, Cr and Cu). The authors have also made a numerical taxonomy study with a hundred resistant strains, using 129 unit characters.
Tipo: Text
Palavras-chave: Microorganisms; Marine environment; Toxicity; Bacteria; Numerical taxonomy; Sediment pollution; Pollution effects; Heavy metals.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1013.pdf

Determination du profil des flores bacteriennes des zones littorales. Application a l'evaluation de l'impact des rejets
Provedor de dados: 5
Autores: Bunetel, L; Segui, A; Guerin, J; Lerman, I; Cormier, M.
The principle of this method is to evaluate, by counts exercised upon selective media, the relative importance of the different bacteria families in the flora of the coastal waters. It is important to separate the contaminant species and the commensal species in the marine environment. Ten culture media distributed into four categories (selective, non selective, saline and non saline) were examined by numeration. Counts were transferred on a diagram allowing to plot down a profile of bacterial flora of water sampled from a given geographical area. The profiles are arranged by a classification method. The sampling points with similar or adjoining profiles were assembled and marked on the chart. In this way, the authors obtained areas representing the impact...
Tipo: Text
Palavras-chave: Bacteria; Coastal waters; Microbiological culture; Sewage disposal; Microbial contamination; Bacterial counters; Bacteria collecting devices.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1004.pdf

Production and consumption of B group vitamins in situ.
Provedor de dados: 5
Autores: Kurata, A.
The changes in concentration of thiamine, biotin and vitamin B sub(12) in water of the soaked bottles for 6 h in situ in the north basin of Lake Biwa were examined successively over 30 hours. The concentrations of the vitamins changed comparatively during 6 h. soaking in situ. Generally, the concentrations of thiamine, biotin and vitamin B sub(12) were in high levels in the morning and in low levels in evening both in the soaked bottles and in the surrounding water. The fluctuation ranges in concentration of vitamins in a 5 m deep layer were greater than those in thermocline. From the data obtained with the soaked bottles in situ, it was supposed that the decrease of vitamins in concentration during the daytime surpass consumption of vitamins by...
Tipo: Text
Palavras-chave: Phytoplankton; Microorganisms; Bacteria; Metabolism; Biological production; Vitamin B; Fresh water.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-958.pdf

Culture-Facilitated Comparative Genomics of the Facultative Symbiont Hamiltonella defensa
Provedor de dados: 5
Autores: Chevignon, Germain; Boyd, Bret M.; Brandt, Jayce W.; Oliver, Kerry M.; Strand, Michael R.
Many insects host facultative, bacterial symbionts that confer conditional fitness benefits to their hosts. Hamiltonella defensa is a common facultative symbiont of aphids that provides protection against parasitoid wasps. Protection levels vary among strains of H. defensa that are also differentially infected by bacteriophages named APSEs. However, little is known about trait variation among strains because only one isolate has been fully sequenced. Generating complete genomes for facultative symbionts is hindered by relatively large genome sizes but low abundances in hosts like aphids that are very small. Here, we took advantage of methods for culturing H. defensa outside of aphids to generate complete genomes and transcriptome data for four strains of...
Tipo: Text
Palavras-chave: Insect; Aphid; Bacteria; Comparative genomics; Parasitoid; Defense.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00635/74665/74590.pdf

Étude des secteurs du Croisic et de Pen-Bé : estimation des apports continentaux et évaluation des stocks conchylicoles
Provedor de dados: 5
Autores: Gendronneau, Maud; Haure, Joel; Jeanneret, Hélène; Le Merrer, Yoann; Lequette, Camille; Lomakine, Cédric; Martin, Jean-louis; Papin, Mathias; Ponthoreau, Catherine; Robin, Marc.
Ifremer, Nantes University and the township community "CAP Atlantique" have joined their skills to try to better understand how two key conchological areas in the shore economy of the Pays de la Loire area develop, especially since, environmentally, little is known about them, and because they face recurrent issues on the zootechnic level. This programme encompasses two main topics: assessment of environmental inputs (catchments areas input, contaminants evolution in the area, and impact on the conchological zones) and a study of the conchological culture (growth, mortality, analysis of the stocks). Concomitantly, operational tools (hydrodynamic and flux transfer models, databases, SIG) are developed to improve local management of the sites and information...
Tipo: Text
Palavras-chave: Phytosanitary; Bacteria; SIG; Conchological stocks; Modelization; Catchment areas; Continental inputs; Pen Bé; Le Croisic; Phytosanitary; Bacteria; SIG; Conchological stocks; Modelization; Catchment areas; Continental inputs; Pen Bé; Le Croisic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-1264.pdf

Microbial extracellular enzyme detection on agar plates by means of fluorogenic methylum-belliferyl-substrates.
Provedor de dados: 5
Autores: Kim, S; Hoppe, H.
A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts.
Tipo: Text
Palavras-chave: Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-959.pdf

Effects of nitrogen limitation on Dunaliella sp.–Alteromonas sp. interactions: from mutualistic to competitive relationships.
Provedor de dados: 5
Autores: Le Chevanton, Myriam; Garnier, Matthieu; Lukomska, Ewa; Schreiber, Nathalie; Cadoret, Jean-paul; Saint-jean, Bruno; Bougaran, Gael.
Interactions between photosynthetic and non-photosynthetic microorganisms play an essential role in natural aquatic environments and the contribution of bacteria and microalgae to the nitrogen cycle can lead to both competitive and mutualistic relationships. Nitrogen is considered to be, with phosphorus and iron, one of the main limiting nutrients for primary production in the oceans and its availability experiences large temporal and geographical variations. For these reasons, it is important to understand how competitive and mutualistic interactions between photosynthetic and heterotrophic microorganisms are impacted by nitrogen limitation. In a previous study performed in batch cultures, the addition of a selected bacterial strain of Alteromonas sp....
Tipo: Text
Palavras-chave: Interactions; Nitrogen limitation; Microalgae; Bacteria; Chemostat; Dissolved organic matter (DOM); Dunaliella.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00344/45529/45087.pdf

Bacteries ichtyopathogenes en mariculture
Provedor de dados: 5
Autores: Vigneulle, Martine.
Bacterial infections occurred during the development of marine fish farming of species such as turbot, sea-bass, sea-bream or salmon and trout. The main-specific bacteria involved in these diseases observed belong to 3 groups: the Vibrionaceae for Vibrio anguillarum (type 408) and Aeromonas salmonicida , the causative agents of respectively vibriosis and furinculosis; the Corynebacteriaceae for Renibacterium salmoninarum , responsible for bacterial kidney disease (BKD), the Enterobacteriaceae for Yersinia ruckeri , the causative agent of enteric redmouth disease. The clinical signs typical of those diseases always depend on the rearing and environmental conditions. The losses resulting from these bacterial diseases and the persistence of the bacteria in...
Tipo: Text
Palavras-chave: Yersinia ruckeri; Renibacterium salmoninarum; Aeromonas salmonicida; Vibrio anguillarum; Bacteria; Bacterial diseases; Fish culture; Pathogenic bacteria.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-996.pdf

Methods for microbial biomass, community structure and metabolic activities on surfaces.
Provedor de dados: 5
Autores: White, D.
Microbes in nature are often concentrated on surfaces where they exist in microcolonies of mixed composition. The assay of these mixed microbial consortia by utilizing biochemical components eliminates the problems associated with cultural selectivity of viable counts or the requirement for quantitative release from surfaces required in some microscopic techniques. The assay of cellular components restricted to subsets of the microbial community gives insights into the community structure of the biofilm whereas assay of cellular components common to all cells can be used for biomass estimations. Formation of endogenous storage materials can be utilized as measures of nutritional status and rates of formation or turnover of specific "signatures" from...
Tipo: Text
Palavras-chave: Bacteria; Fouling; Bioassays; Metabolism; Surfaces; Bacteria collecting devices; Culture media; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-963.pdf

Implantation et survie de Campylobacter jejuni/coli chez la moule Mytilus edulis
Provedor de dados: 5
Autores: Minet, Jacques; Sauvager, F; Cormier, M.
Illnesses due to marine bivalvia consumption are mostly associated with viruses as causative agents. In more than the half of cases, this agent remains unidentifies, nevertheless. So far as bacteria are concerned, Salmonella are found firstly. Campylobacters represent a major cause of gastrointestinal disease, with Salmonella, among humans. Whether campylobacteriosis may be acquired from shellfishes remains unclear. We undertook to comparatively appreciate the survival of Campylobacter jejuni/coli and Salmonella typhimurium in seawater and contaminated mussels. Bacterial enumerations where performes using microbial cultures and specific (with immunofluorescence) direct viable counts (of substrate responsive bacteria). During laboratory contamination...
Tipo: Text
Palavras-chave: Campylobacter coli; Campylobacter jejuni; Mytilus edulis; Bivalvia; Pathogenic bacteria; Seafood; Microbial contamination; Pollution effects; Bacteria; Sea water; Survival.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1606.pdf

Assessment of viability in the bacterial standing stock of the Antarctic sea from the Indian side
Provedor de dados: 5
Autores: Loka Bharathi, Pa; Nair, S; Bd De Souza, Mj; Chandramohan, D.
During the austral summer, we examined the bacterial population along the cruise track extending from 70degrees S and 18degrees E to 30degrees S and 35degrees E. During the cruise, three distinct fractions of the bacterioplankton viz. total count, total direct viable and retrievable counts were simultaneously enumerated in these waters. In the stations south of the convergent region designated as section I the population ranged within 10(8-9) L-1 whereas in the north of the region designated as section 11 they were one order higher and ranged from 10(9-10) L-1. The percentage of viability in the region was high corresponding to the generally high chlorophyll and primary productivity encountered in the eastern Aghulas bank. The study substantiates the...
Tipo: Text
Palavras-chave: Bactérie; Viabilité; Océan Antarctique; Secteur indien; Bacteria; Viability; Antarctic ocean; Indian side.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00322/43334/42893.pdf

Ecologie des vibrions pathogenes
Provedor de dados: 5
Autores: Dodin, A; Dosso, M; Guillou, M.
There exists no biochemical test allowing to define the pathogenicity of a bacterium, still less for a Vibrionaceae. Two essential characters are to be retained to hold responsible an isolated germ, for an illness: 1) the binding factor, the identification is difficult but a double diffusion of agar should simplify the method; 2) the search for the choleric toxin or a common part of the choleric toxin (toxin LT or ST of E. coli ). Here again, simplified techniques may be used. The acquirement of a plasmid responsible for the binding factor or for the toxin production of the non-pathogenic Vibrionaceae in the origin of certain epidemic diseases in France and in the world. Frequently, epidemics due to common germs (Aeromonas hydrophila of Africa) lead to...
Tipo: Text
Palavras-chave: Aeromonas hydrophila; Vibrionaceae; Bacteria; Ecology; Microorganisms; Hosts; Bacterial diseases; Pathogenic bacteria.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-999.pdf

Rapid method for determining the sensitivity of sulphate-reducing bacteria to biocides.
Provedor de dados: 5
Autores: Sharma, A; Battersby, N; Stewart, D.
The Minimal Inhibitory Concentration (MIC) of a biocide is usually determined by adding it in various concentrations to a suitable growth medium which is then inoculated with the test bacterium. These cultures are then observed for up to 28 days for growth of the organism as shown by its production of black ferrous sulphide. A method has been developed whereby the MIC of a biocide can be found within 4 days. Instead of noting ferrous sulphide production, this new method measures changes in: 1) optical density and 2) sulphate reduction of a logarithmic culture of the test strain exposed to the biocide.
Tipo: Text
Palavras-chave: Bacteria; Microorganisms; Pesticides; Analytical techniques; Sulfate reduction.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1016.pdf

Estimates of carbon flow through bacteriplankton in the S. Benguela upwelling region based on 3H-thymidine incorporation and predator-free incubations.
Provedor de dados: 5
Autores: Lucas, M; Painting, S; Muir, D.
Spatial and temporal estimates of bacterial numbers, biomass, activity and production were measured in the S. Benguela upwelling system during the course of a phytoplankton bloom. Bacterial numbers and biomass were highest in the euphotic zone and correlated closely with particulate carbon in the water column rather than with chlorophyll a concentrations. 3H-Thymidine incorporation gave good estimates of production. Differential utilisation of photosynthetic exudates and detrital POC by bacterioplankton during the course of a bloom might account for the varying significance ascribed to exudates and POC as a source of carbon for bacterial production.
Tipo: Text
Palavras-chave: Ecological associations; Algal blooms; Biomass; Microorganisms; Carbon cycle; Bacteria; Nannoplankton; Upwelling; Biogeochemistry.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-985.pdf

Devenir des bactéries dans les réseaux trophiques des vasières intertidales : le cas de brouage (Baie de Marennes-Oléron)
Provedor de dados: 5
Autores: Pascal, Pierre-yves.
Bacteria are highly abundant and productive in intertidal mudflat sediments. By analogy  with pelagic system, it has been suggested that bacteria play an important role in benthic food  web. Due to technical difficulties, numbers of studies dealing with benthic bacterivory are not  numerous and give conflicting results. A method using community of 15N enriched bacteria as  tracer was developed in order to measure grazing of meiofauna and macrofauna. Since enriched  bacteria present characteristics of size, activity and diversity close to natural ones, bias due to  selective bacterial ingestion by grazers are limited. Due to tidal cycles, intertidal mudflat  constitutes highly variable environment at short time scale. Since those variations may influence...
Tipo: Text
Palavras-chave: Stable isotope; Hydrobia ulvae; Foraminifera; Copepod; Nematode; Bacteria; Mudflat; Trophic foodweb; Isotope stable; Hydrobia ulvae; Foraminifère; Copépode; Nématode; Bactérie; Vasière; Réseau trophique.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-4778.pdf

Growth Performance and Adaptability of European Sea Bass (Dicentrarchus labrax) Gut Microbiota to Alternative Diets Free of Fish Products
Provedor de dados: 5
Autores: Pérez-pascual, David; Estellé, Jordi; Dutto, Gilbert; Rodde, Charles; Bernardet, Jean-françois; Marchand, Yann; Duchaud, Eric; Przybyla, Cyrille; Ghigo, Jean-marc.
Innovative fish diets made of terrestrial plants supplemented with sustainable protein sources free of fish-derived proteins could contribute to reducing the environmental impact of the farmed fish industry. However, such alternative diets may influence fish gut microbial community, health, and, ultimately, growth performance. Here, we developed five fish feed formulas composed of terrestrial plant-based nutrients, in which fish-derived proteins were substituted with sustainable protein sources, including insect larvae, cyanobacteria, yeast, or recycled processed poultry protein. We then analyzed the growth performance of European sea bass (Dicentrarchus labrax L.) and the evolution of gut microbiota of fish fed the five formulations. We showed that...
Tipo: Text
Palavras-chave: European sea bass; Fish gut microbiota; Bacteria.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00646/75830/76816.pdf

Etude au microscope electronique a balayage des relations entre bacteries et matieres en suspension en milieu marin
Provedor de dados: 5
Autores: Cormier, M; Bassoulet, P; Pommepuy, Monique.
Near outfall areas numerous bacteria appear associated to colloidal organic matter present in littoral sea water. Arrangements into colonies are shown. Surface structures are observed which allow bacteria's attachment each to other and to organic matter.
Tipo: Text
Palavras-chave: Bacteria; Outfalls; Electron microscopy; Flocculation; Colloids; Adhesion; Suspended particulate matter.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-966.pdf

Transit bacterien dans le tube digestive de la truite arc-en-ciel
Provedor de dados: 5
Autores: Lesel, R; Fauconneau, B; Alzieu, P.
The present study concerns bacterial transit in the digestive tract of rainbow trout at 13 degree C and compares fecal excretion of 4 tracers in the alimentary bolus, an inert bacterial tracer: spores of Bacillus stearothermophilus , a bacterial marker capable of multiplying in the digestive organs: Citrobacter freundii ; a tracer of the liquid phase: polyethylene glycol (PEG), a tracer of the solid phase: Cr sub(2)O sub(3). Two peaks of fecal excretion of bacterial bodies are indicated in the results, the first one taking place within the first 4 h following ingestion of the traced meal, the second peak, a hundred times more important, occurring 10 h after the meal. This two-phase mode of excretion corroborates the observations previously made. The...
Tipo: Text
Palavras-chave: Citrobacter freundii; Bacillus stearothermophilus; Bacteria; Excretion; Tracer techniques.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-995.pdf

Ballast water research in France : Current status
Provedor de dados: 5
Autores: Masson, Daniel; Courtois, Olivier; Masson, Nadine; Guesdon, Stephane; Rocher, Gregory.
Due to important economic activities along the French oastline, the introduction of noxious organisms into French coastal waters, particularly by ship ballast water, may have serious consequences. After preliminary studies of ship movements in Charente Maritime, several samplings of ballast water and sediment were made. Samples were taken in five major harbours in France, along the Atlantic, Channel and Mediterranean coastlines. The main task of this first assessment was the research for exotic and possibly noxious phytoplancton. Clostridium and Vibrio bacteria were also tested for. The results are presented and discussed, with plans for further work.
Tipo: Text
Palavras-chave: Bacteria; Phytoplancton; French coastal waters; Ballast water.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-7364.pdf

Taxonomy and distribution of hydrocarbonoclastic bacteria from the Ixtoc-1 area.
Provedor de dados: 5
Autores: Lizarraga, P; Munoz, R; Porras, A; Izquierdo, V; Wongchang, I.
A bacteriological monitoring program has been established in order to evaluate the impact of oil activities. The ratio of heterotrophic-hydrocarbonoclastic bacteria was used to follow the evolution in time of both populations. Numerical taxonomic methods were employed in order to define ecotypes of marine oil degraders.
Tipo: Text
Palavras-chave: Pollution monitoring; Blowouts; Bacteria; Heterotrophic organisms; Biodegradation; Oil pollution.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1019.pdf

Subseafloor archaeal communities: from the surface to a deep hot biosphere?
Provedor de dados: 5
Autores: Roussel, Erwan.
The sub-seafloor biosphere may contain two thirds of Earth's total prokaryotic biomass. The large and active microbial populations buried in the sub-seafloor play a key role in global biogeochemical cycles. However, little is known about these prokaryotic communities. The depth limit of this sub-seafloor biosphere is still unreached, and elevated temperatures as well as insufficient energy sources are the likely factors limiting life at depth. Interestingly, archaeal communities with unknown physiologies and no cultured relatives seem to have a key role in deep marine sediments and hydrothermal ecosystems.  As most deep biosphere microorganisms detected so far have been extremely resistant to cultivation, molecular approaches remain to date the most...
Tipo: Text
Palavras-chave: Sediment; Hydrothermal system; Ultramafic; CM DGGE; DGGE; PmoA; DsrA; McrA; AmoA; 16SrRNA; Bacteria; Archaea; Deep biosphere; Sédiment; Écosystème hydrothermal; Ultramafique; CM DGGE; DGGE; PmoA; DsrA; McrA; AmoA; 16SrRNA; Bacteria; Archaea; Biosphère profonde.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-4630.pdf

Influence de l'environnement trophique de l'élevage en biofloc sur les performances physiologiques de la crevette Litopenaeus stylirostris : Étude de paramètres de la nutrition, de l'immunité et de la reproduction
Provedor de dados: 5
Autores: Cardona, Emilie.
Biofloc is an intensive rearing system with zero or minimal water exchange where a diverse population of microorganisms (microalgae, zooplankton and bacteria) develops in association with organic matter to form the floc particles. These particles play the double role of biological filter and dietary supplement. This dissertation aims to better understand the process of this rearing system and its interactions with the Litopenaeus stylirostris shrimp. Two specific objectives were integrated within the framework of this general objective: (i) to measure the production gain from biofloc rearing and (ii) to study the interaction between biofloc environment and shrimp and to assess its role on production performances of shrimps. Thus, our results show...
Tipo: Text
Palavras-chave: Biofloc; Crevette; Nutrition; Stress oxydant; Défenses antioxydantes; Immunité; Reproduction; Oeufs; Larves; Bactéries; Microbiote; Biofloc; Shrimp; Nutrition; Oxidative stress; Antioxidant defences; Immunity; Reproduction; Eggs; Larvae; Bacteria; Microbiota.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00272/38294/36492.pdf

Evolution des caractéristiques chimiques et biologiques des sédiments en circuit clos. I. Azote et populations bactériennes en fonction de certains paramètres du milieu
Provedor de dados: 5
Autores: Chamroux, S.; Boucher, G.
Chemical and Biological evolution of sediments in closed circuit : I. Nitrogen and bacteria. A series of well controlled sea water closed circuit sand filters al­lowed the study of chemical and biological regulation processes in the sediment. Bacteria, Nitrogen chemistry and meiofauna were mainly investigated. The evolution of the different forms of nitrogen is rather similar, whatever the organic matter flow in the sediment. As long as the amount of or­ganic input works as limiting factor, bacteria densities are proportional to its flow. No correlation was found between bacteria and chemical parameters in the sand or in the water. The temperature does not seem to play a determinant part neither in the evolution of bacteria nor in the mineralization of...
Tipo: Text
Palavras-chave: Circuit clos; Sédiment; Bactéries; Matière organique; Azote; Closed circuit; Sediment; Bacteria; Organic matter; Nitrogen.
Ano: 1979
URL: http://archimer.ifremer.fr/doc/00305/41652/40861.pdf

Transferts d'acides amines dans un systeme eau-sediment: Etude preliminaire en milieu experimental
Provedor de dados: 5
Autores: Hermin, M; Daumas, R; Drogue, N.
The bacterial activity of the superficial sediment layer was evaluated by incorporation super(14)C-labelled amino-acids mixed an hydrolysate of chlorella cells. Estimation of super(14)C-CO sub(2) super(14)C and super(14)C-A.A. by H.P.L.C. give some informations on the utilization of free dissolved. A.A. and on the transport of labelled compounds from water to sediment. The adsorption mechanism of A.A. on the mineral particles draw to the sediment a larger part of A.A. in the first period; but 28% of this adsorbed A.A. were discharged by an acid treatment. A part of super(14)C-A.A. was incorporated in unhydrolysable organic matter. Di-COOH A.A. are more resistant to bacterial degradation than the other A.A., but arginine is completely metabolized in ten...
Tipo: Text
Palavras-chave: Biogeochemistry; Bacteria; Chromatographic techniques; Metabolism; Amino acids; Sediment water exchanges.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-945.pdf

Tepidibacter formicigenes sp nov., a novel spore-forming bacterium isolated from a Mid-Atlantic Ridge hydrothermal vent
Provedor de dados: 5
Autores: Urios, Laurent; Cueff, Valerie; Pignet, Patricia; Barbier, Georges.
A novel anaerobic, Gram-positive, sporulating and strictly chemoorganoheterotrophic bacterium, designated strain DV1 184 T, was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were short, straight rods (4 mum long and 0.8 mum wide) and were motile with peritrichous flagella. They grew between 35 and 55 degreesC (optimum, 45 degreesC), between pH 5.0 and 8.0 (optimum, 6.0) and at 20-60 g sea salts l(-1) (optimum sea salts concentration, 30 g l(-1)). Strain DV1184(T) was able to ferment yeast extract, tryptone, peptone, glucose, sucrose, maltose and pyruvate. The main fermentation products from glucose were (in decreasing order) formate, acetate and ethanol. The genomic DNA G+C content was 29 mol%. Phylogenetic...
Tipo: Text
Palavras-chave: Phylogeny; Bacteria; Deep sea hydrothermal vent.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-589.pdf

Construction and use of a dialysis chamber for investigating in situ the toxicity of heavy metals on bacteria.
Provedor de dados: 5
Autores: Tan, T.
Teflon dialysis sampling chambers have been employed to study the toxicity of ZnCl sub(2) on a cadmium-sensitive bacterium, Acinetobacter) CS 13, in an aquarium with running seawater. A concentration of 10 mg Zn super(++) l super(-1) did not influence the cell densities in the chambers, but cell densities decreased at zinc concentrations of 15 and 20 mg l super(-1). In seawater with 20 mg Zn super(++) lu-1 the bacteria accumulated zinc from 827 to 2813 mu g g super(-1) cells dry wt after 1 d. After 2 d the zinc concentration increased to 4625 mu g g super(-1) dry wt. Three chambers were filled with Acinetobacter CS 13 cells added to sterile sediment at a concentration of 1 x 10 super(8) cells ml super(-1) wet sediment. To test the security, these chambers...
Tipo: Text
Palavras-chave: Acinetobacter; Bacteria; Laboratory culture; Tests; Zinc; Dialysis; Toxicity; Heavy metals.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1012.pdf

Sampling for a bacteriological study of aquatic enrironments
Provedor de dados: 5
Autores: Troussellier, M; Baleux, B; Andre, P.
Using water samples from several observation levels of a brackish lagoon, statistical solutions are offered for the sampling optimisation of bacteria viable counts. The variability between plate count is studied by the mean of the Taylor's power law. The sampling strategy optimisation, including several sources of variability, is realized by hierarchical analysis of variance and the estimation of the sampling cost of each source. This optimisation procedure results in a great increase of the relative efficiency of the sampling design and allow to obtain significant differences between the bacterial concentrations. The effect of the reduction of the total sampling effort on the precision of these average concentrations pointed out the scientific and...
Tipo: Text
Palavras-chave: Bacteria; Abundance; Variance analysis; Microbiological analysis; Lagoons; Brackishwater environment; Bacterial counters; Biological sampling.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1984/acte-941.pdf

Mesoscale surface distribution of biogeochemical characteristics in the Crozet Basin frontal zones (South Indian Ocean)
Provedor de dados: 5
Autores: Fiala, M; Delille, B; Dubreuil, C; Kopczynska, E; Leblanc, K; Morvan, J; Queguiner, B; Blain, S; Cailliau, C; Conan, P; Corvaisier, R; Denis, M; Frankignoulle, M; Oriol, L; Roy, S.
A mesoscale study was conducted in January and February 1999 in the Crozet Basin frontal zones (43degrees50' to 45degrees20'S, 61degrees00' to 64degrees30'E) within the southernmost and easternmost convergence area of the Antarctic Circumpolar Current (ACC) and the Agulhas Return Current (ARC). Distribution of biogeochemical parameters was strongly linked to the merged Subtropical (STF) and Subantarctic (SAF) Fronts which mark the border between the cold and less saline subantarctic waters and the warm and more saline subtropical waters. This survey took place during a post-bloom period. Chlorophyll a concentrations were low throughout the study area ranging from 0.2 mug l(-1) in the Polar Frontal Zone (PFZ) to 0.4 mug l(-1) in the Subtropical Zone (STZ)....
Tipo: Text
Palavras-chave: Frontal zones; Nutrients; Biogenic silica; Chlorophyll a; PCO(2); Phytoplankton; Bacteria.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/00224/33557/31968.pdf

Les chaines alimentaires naturelles et l'aquaculture
Provedor de dados: 5
Autores: Barnabe, G.
Aquaculture sets up manipulation of natural food chains. This paper describes some utilizations of photosynthetic bacteria, phytoplankton and zooplankton taken out sewage ponds, wetlands or neritic waters.
Tipo: Text
Palavras-chave: Bacteria; Photosynthesis; Aquaculture; Ecosystems; Zooplankton; Phytoplankton; Food chains.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1254.pdf

Comparaison de methodes directes (microscopiques) et indirectes (par mise en culture) dans l'evaluation d'une pollution bacterienne d'origine fecale. Etude preliminaire
Provedor de dados: 5
Autores: Munro, P; Bianchi, M.
Cells of two serotypes of Escherichia coli (O26B6 and 0111B4) were counted using epifluorescence and immunofluorescence microscopy (nonavalent serum) and plate count on specific medium. Values from indirect counts were 10 fold smaller than those from both microscopic counts. In a sample of sea water from an area receiving a fecal pollution, the highest number of bacteria was obtained by epifluorescence microscopy. In that case, the number of cells counted by immunofluorescence microscopy was 10 fold smaller than the number of bacteria able to grow on the medium. It could be due to: the diversity of the natural community, the possibility to other bacteria than E. coli to grow on the used culture medium, the restrictiveness of the serum for "pathogenic...
Tipo: Text
Palavras-chave: Escherichia coli; Bacteria; Microbiological culture; Pathogenic bacteria; Microbial contamination; Bacterial counters; Fluorescence microscopy; Bacteria collecting devices; Indicator species.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1001.pdf

Seasonal development of bacterial communities in a coastal marine sediment as related to the input of organic material.
Provedor de dados: 5
Autores: Meyerreil, L; Graf, G.
The seasonal development of benthic communities was followed in a coastal marine sediment of the Kiel Bight (Baltic Sea; FRG). Total benthic biomass, (ATP), bacterial biomass, overall benthic activity (heat production), and enzymatic decomposition rates of carbohydrate (d-amylase activity) followed a seasonal cycle strongly related to certain events in the sediment. Among these, the input of the phytoplankton blooms in autumn and spring, the accumulation of organic material during winter, and changes in the physical characteristics of the sediment turned out to be most important for the development of benthic biomass and activity. Processes within the benthic community occurred in very short time scales (within days).
Tipo: Text
Palavras-chave: Bacteria; Organic matter; Sediments; Bioenergetics; Enzymatic activity; Biomass.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-944.pdf

Importance of ecological interactions during wastewater treatment using High Rate Algal Ponds under different temperate climates
Provedor de dados: 5
Autores: Galès, Amandine; Bonnafous, Anaïs; Carré, Claire; Jauzein, Vincent; Lanouguère, Elodie; Le Floc'H, Emilie; Pinoit, Joanna; Poullain, Clothilde; Roques, Cécile; Sialve, Bruno; Simier, Monique; Steyer, Jean-philippe; Fouilland, Eric.
Several studies focused on wastewater treatment in High Rate Algal Ponds (HRAP) suggest that highly variable climatic conditions cause large variations of microalgal biomass productivity. In the present study, we show that similar carbon, nitrogen and phosphorus removal efficiencies were reached in different HRAPs treating urban wastewaters located in two different temperate (Mediterranean and oceanic) climates. Furthermore, similar ecological successions were observed in these HRAPs. During the start-up phase, the consumption of organic matter by detritivores, already present in the wastewater, appears to be necessary for the microalgae to grow within two weeks in spring. The growth of the rapid-growing species, Chlorella sp., followed by the...
Tipo: Text
Palavras-chave: Microalgae; Predators; Bacteria; Urban effluents; Ammonia and phosphate removal.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00490/60210/63713.pdf

Effects of temperature abuse on survival ofVibrio vulnificus in oysters
Provedor de dados: 5
Autores: Murphy, S; Oliver, J.
Of the several bacterial diseases which may result from consumption of shellfish, those caused by marine bacteria of the genus Vibrio are the most abundant. In the United States, up to 10,000 non-fatal cases per year are estimated to be caused by the various members of this genus. More than 95% of all deaths in the United States which result from seafood consumption are caused by a single bacterium, Vibrio vulnificus. The bacterium is a normal inhabitant of estuarine waters, and occurs naturally in especially high numbers in molluscan shellfish. Infections following consuption of raw or undercooked shellfish, especially oysters, result in fatality rates of over 60%. Because most oysters in the United States are transported large distances before marketing,...
Tipo: Text
Palavras-chave: USA Coasts; Vibrio cholerae; Vibrio vulnificus; Crassostrea virginica; Bivalvia; Bacteria; Temperature effects; Food technology; Fish poisoning; Oyster culture.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1622.pdf

Analyse bacteriologique de l'eau par mesure potentiometrique de la reduction de l'acide lipoique
Provedor de dados: 5
Autores: Charriere, G; Jouenne, T; Junter, G.
A simple potentiometric technique to detect and enumerate the bio-indicators of water contamination is described. The technique consists in measuring the redox potential of a specific culture medium saturated with oxygen where bacteria are incubated in presence of lipoic acid (LA). These micro-organisms reduce LA to dihydrolipoic acid, giving rise to an important potential drop in the system. This potentiometric "signal" reveals the presence of active metabolic bacteria in the medium. The major assets of potentiometric measurements in presence of LA lies in the reliability and intelligibility of the results (the redox phenomenon being qualitatively and quantitatively studied), associated to a very simple technology. Futhermore, this technique is perfectly...
Tipo: Text
Palavras-chave: Bacteria; Pollution indicators; Water quality; Microbial contamination; Indicator species; Pollution detection; Redox potential; Titration.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1007.pdf

The nutritive value of the detritus for the marine plankton animals
Provedor de dados: 5
Autores: Pechen-finenko, G.
The author reviews the scientific works dealing with the role and the nutritive value of detritus particles for zooplankton.
Tipo: Text
Palavras-chave: Bacteria; Nutrition; Zooplankton; Detritus; Nutritive value.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1529.pdf

Relations between bacterial extracellular enzyme activities and heterotrophic substrate uptake in a brackishwater environment.
Provedor de dados: 5
Autores: Hoppe, H.
Extracellular enzymes mediate the decomposition of polymeric organic compounds in natural waters. In many cases these enzymes comprise a component linked to the bacterial fraction of the aquatic community and react in close association with these cells. An annual survey of microbial activities in a brackish water fjord exhibited an excellent correlation between V sub(m) of proteases (ability to split the substrate analogue methylumbelliferyl-leucine) and V sub(m) as well as T sub(R) for leucine uptake by microorganisms. Hydrolysis rates (H sub(R)) for the decomposition of naturally occurring competitive substrate analogues of the model substrates (methylumbelliferyl-(MUF)-leucine, MUF- alpha -D-glucose, MUF-N-acetyl-glucosamine, MUF-phosphate) drastically...
Tipo: Text
Palavras-chave: Biogeochemistry; Fjords; Bacteria; Brackishwater environment; Seasonal variations; Dissolved organic matter; Interspecific relationships; Hydrolysis; Enzymes.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-952.pdf

Bacterivory in the common foraminifer Ammonia tepida: Isotope tracer experiment and the controlling factors
Provedor de dados: 5
Autores: Pascal, P.y.; Dupuy, C; Richard, P; Niquil, N.
The majority of sediment dweller foraminifera are deposit feeders. They use their pseudopodia to gather sediment with associated algae, organic detritus and bacteria. Uptake of bacteria by foraminifera have been observed but rarely quantified. We measured uptake of bacteria by the common foraminifera Ammonia tepida using 15N pre-enriched bacteria as tracers. In intertidal flats, seasonal, tidal and circadian cycles induce strong variations in environmental parameters. Grazing experiments were performed in order to measure effects of abiotic (temperature, salinity and irradiance) and biotic (bacterial and algal abundances) factors on uptake rates of bacteria. In mean conditions, A. tepida grazed 78 pgC ind-1 h-1 during the first eight hours of incubation,...
Tipo: Text
Palavras-chave: Trophoecology; Prey abundance; Mudflat; Grazing; Foraminifera; Environmental factor; Bacteria.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4298.pdf

Maintenance of discrete bacterial populations in adjacent marine habitats.
Provedor de dados: 5
Autores: Robb, F; Muir, D; Davis, C.
Some mechanisms of selection which operate on marine Vibrio and Pseudomonas strains during survival in dilute environments have been examined. Mannitol uptake and utilisation systems are more efficient in the Vibrio strain compared with the Pseudomonas strain during starvation survival. A Vibrio mutant strain lacking mannitol uptake showed no greater loss of viability during starvation survival than mannitol positive strains. The Vibrio strain appears to be constitutive with respect to mannitol uptake systems, whereas the Pseudomonas strain examined is inducible. Active uptake systems for glucose, glutamate, alanine and aspartic acid were efficiently maintained during starvation survival in the Vibrio strain. Results suggest that membrane uptake systems...
Tipo: Text
Palavras-chave: Pseudomonas; Vibrio; Bacteria; Physiology; Alcohols; Food conversion; Survival; Starvation; Metabolism.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-960.pdf

Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance
Provedor de dados: 5
Autores: Cruz, Kimberly; Guezennec, Jean; Barkay, Tamar.
Bacteria employ adaptive mechanisms of mercury (Hg) tolerance to survive in environments containing elevated Hg concentrations. The potential of extracellular polysaccharides (EPS) production by bacteria as a mechanism of Hg tolerance has not been previously investigated. The objectives of this study were to determine if bacterial EPS sorb Hg, and if so does sorption provide protection against Hg toxicity. Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption. The data showed that EPS sorbed up to 82% of Hg from solution, that this sorption was dependent on EPS composition, and that sorption was a saturable mechanism. Hg...
Tipo: Text
Palavras-chave: Mercury; Bacteria; Sorption; Tolerance; Exocellular polysaccharides.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00382/49348/49786.pdf

Isolement et identification de vibrions halophiles d'une station experimentale de mariculture
Provedor de dados: 5
Autores: Bruni, V; Crisafi, E; Maugeri, T; Zaccone, R.
110 Vibrio strains were isolated, at various times of the year, from samples of sea-water supply, breeding-tank water and discarded fish used as food at the breeding stations. From the results obtained by the biochemical and cultural tests, it was possible to identify most of the isolated strains as Vibrio anguillarum . Strains with intermediate characteristics, above-all positive saccharose, proved to be numerous. The authors discuss the occurrence, in particular environments such as those being studied, of atypical or intermediate vibrio strains which are difficult to classify.
Tipo: Text
Palavras-chave: Vibrio; Microbiological culture; Bacteria; Food; Minced products; Fish ponds; Sea water; Identification; Marine aquaculture; Bacteria collecting devices; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-997.pdf

Techniques de cultures axeniques d'organismes planctoniques: Un rotifere, Brachionus plicatilis et une algue, Platymonas (= Tetraselmis ) suecica.
Provedor de dados: 5
The efficiency of different methods for the purification and maintenance of axenic cultures of a rotifer and an unicellular alga is tested. The use of antibiotics seems to be the most suitable method to realize bacteria-free cultures of plankton. No elements prove that the presence of bacteria is necessary for the development of the cultures. Bacteria-free cultures show a physiological evolution of the culture medium is enriched with some nutrients.
Tipo: Text
Palavras-chave: Brachionus plicatilis; Rotifera; Algae; Bacteria; Antibiotics; Algal culture; Laboratory culture.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/publication-1823.pdf

Phytoplankton and bacterial alkaline phosphatase activities in relation to phosphate and DOP availability within the Gironde plume waters (Bay of Biscay)
Provedor de dados: 5
Autores: Labry, Claire; Delmas, Daniel; Herbland, Alain.
Previous studies conducted on the continental shelf in the Southeast Bay of Biscay influenced by Gironde waters (one of the two largest rivers on the French Atlantic coast) showed the occurrence of late winter phytoplankton blooms and phosphorus limitation of algal growth thereafter. In this context, the importance of dissolved organic phosphorus (DOP) for both algae and bacteria was investigated in 1998 and 1999 in terms of stocks and fluxes. Within the mixed layer, although phosphate decreased until exhaustion from winter to spring, DOP remained high and phosphate monoesters made up between I I to 65% of this pool. Total alkaline phosphatase activity (APA, V-max) rose gradually from winter (2-8 nM h(-1)) to late spring (100-400 nM h-1), which was mainly...
Tipo: Text
Palavras-chave: Phytoplankton; Phosphate; Gironde; DOP; Bacteria; Alkaline phosphatases.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-608.pdf

A laboratory model system for analysing microbial interactions occurring in anoxic estuarine sediments.
Provedor de dados: 5
Autores: Herbert, R; Keith, S.
Defined mixed populations of Clostridium butyricum, Desulfovibrio desulfuricans and Chromatium vinosum co-existed for long periods when grown in a single stage chemostat with glucose as sole carbon source. The nature and availability of the inorganic nitrogen source exerted a major effect on carbon flow in the experimental system. The data show that both the C. butyricum and D. desulfuricans isolates can utilise NO sub(3) as an e super(-)acceptor with an increase in cell yield. Under these growth conditions the free S super(2-) levels were lower resulting in more stable mixed populations. In addition the data show that the C. butyricum produces more oxidised fermentation end-products (acetate) when NO sub(3) super(-) was available and more reduced products...
Tipo: Text
Palavras-chave: Chromatium vinosum; Desulfovibrio desulfuricans; Clostridium butyricum; Biogeochemistry; Models; Bacteria; Detritus; Mineralization; Anoxic sediments; Estuarine sedimentation; Analytical techniques; Carbon cycle; Interspecific relationships; Microbiology.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-947.pdf

Models of relations between bacteria and plankton, and regeneration processes in marine planktonic ecosystem.
Provedor de dados: 5
Autores: Martin, Y; Lelong, P; Cauchi, B.
In order to study bacteria-plankton relationships, experimental and mathematical models were used to stimulate biological processes implicated in plankton ecosystems and to analyze community relationships. The model components are phytoplankton, zooplankton, autotrophic and heterotrophic bacteria, ciliates, nutrients, organic matter and phytoplankton antibiotics. This study shows the importance of some factors affecting bacteria communities dynamics and the bacterial activity in mineralization process. Bacterial populations seem to be controlled essentially by the concentration and chemical composition of organic substrates. Antibiosis and predation show a lesser importance on changes in bacteria communities.
Tipo: Text
Palavras-chave: Biogeochemistry; Bacteria; Microorganisms; Mathematical models; Mineralization; Ecosystems; Plankton; Ecological associations.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-982.pdf

Dynamique du phosphore et rôle comme facteur limitant de la production planctonique dans le panache de la Gironde sur le plateau continental Sud Gascogne
Provedor de dados: 5
Autores: Labry, Claire.
This study was supported by the Atlantic working site of the « Programme National d’Océanographie Côtière » (PNOC, PNEC since 1999). The objectives were (1) to check the existence and to understand the mechanisms of winter phytoplankton blooms in the bay of Biscay influenced by the Gironde waters (2) to determine the limiting nutrient of the primary production associated to these blooms and to follow the seasonal evolution of the limiting nutrient, (3) to understand the consequences of this early limitation on the structuration of algal populations in spring. Since phosphorus was supposed to be the major limiting nutrient of algal growth in the Gironde plume, the different forms of the phosphorus cycle as well as the bacterial compartment, central in this...
Tipo: Text
Palavras-chave: Golfe de Gascogne; Gironde; Phytoplancton; Bactéries; Phosphates; Phosphore organique; Dissous; Phosphatases; Bay of Biscay; Gironde; Phytoplankton; Bacteria; Phosphate; Dissolved organic phosphorus; Phosphatases.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00013/12463/9298.pdf

Bacteries associees aux production de Brachionus plicatilis
Provedor de dados: 5
Autores: Nicolas, Jean-louis; Joubert, M.
Bacteria associated with semi-continuous culture of Brachionus plicatilis , which is used as the first food of marine fish larvae, were counted on Zobell 2216 E medium and identified. The numbers of bacteria were very high in both Brachionus (about 10 super(11) bact. per ml of digestive content) and in water (from 1.6.10 super(6) to 4.7.10 super(7) bact. per ml). The cultures were studied at driving a time when mortalities occurred frequently. The introduction of a Pseudomonas) phenon at a proportion of about 50% to the dominant microflora persisted in time and was little influenced by diet. It has been the cause of the repression of the Vibrio, Aeromonas group which was found in an unusually small proportion. It induced a lack of microflora diversity and...
Tipo: Text
Palavras-chave: Brachionus plicatilis; Rotifera; Pseudomonas; Aeromonas; Vibrio; Nutrition; Pathology; Fish culture; Fish larvae; Food organisms; Bacteria; Continuous culture; Ecological associations; Zooplankton culture.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-994.pdf

The ocean sampling day consortium
Provedor de dados: 5
Autores: Kopf, Anna; Bicak, Mesude; Kottmann, Renzo; Schnetzer, Julia; Kostadinov, Ivaylo; Lehmann, Katja; Fernandez-guerra, Antonio; Jeanthon, Christian; Rahav, Eyal; Ullrich, Matthias; Wichels, Antje; Gerdts, Gunnar; Polymenakou, Paraskevi; Kotoulas, Giorgos; Siam, Rania; Abdallah, Rehab Z.; Sonnenschein, Eva C.; Cariou, Thierry; O'Gara, Fergal; Jackson, Stephen; Orlic, Sandi; Steinke, Michael; Busch, Julia; Duarte, Bernardo; Cacador, Isabel; Canning-clode, Joao; Bobrova, Oleksandra; Marteinsson, Viggo; Reynisson, Eyjolfur; Loureiro, Clara Magalhaes; Luna, Gian Marco; Quero, Grazia Marina; Loescher, Carolin R.; Kremp, Anke; Delorenzo, Marie E.; Ovreas, Lise; Tolman, Jennifer; Laroche, Julie; Penna, Antonella; Frischer, Marc; Davis, Timothy; Katherine, Barker; Meyer, Christopher P.; Ramos, Sandra; Magalhaes, Catarina; Jude-lemeilleur, Florence; Leopoldina Aguirre-macedo, Ma; Wang, Shiao; Poulton, Nicole; Jones, Scott; Collin, Rachel; Fuhrman, Jed A.; Conan, Pascal; Alonso, Cecilia; Stambler, Noga; Goodwin, Kelly; Yakimov, Michael M.; Baltar, Federico; Bodrossy, Levente; Van De Kamp, Jodie; Frampton, Dion M. F.; Ostrowski, Martin; Van Ruth, Paul; Malthouse, Paul; Claus, Simon; Deneudt, Klaas; Mortelmans, Jonas; Pitois, Sophie; Wallom, David; Salter, Ian; Costa, Rodrigo; Schroeder, Declan C.; Kandil, Mahrous M.; Amaral, Valentina; Biancalana, Florencia; Santana, Rafael; Pedrotti, Maria Luiza; Yoshida, Takashi; Ogata, Hiroyuki; Ingleton, Tim; Munnik, Kate; Rodriguez-ezpeleta, Naiara; Berteaux-lecellier, Veronique; Wecker, Patricia; Cancio, Ibon; Vaulot, Daniel; Bienhold, Christina; Ghazal, Hassan; Chaouni, Bouchra; Essayeh, Soumya; Ettamimi, Sara; Zaid, El Houcine; Boukhatem, Noureddine; Bouali, Abderrahim; Chahboune, Rajaa; Barrijal, Said; Timinouni, Mohammed; El Otmani, Fatima; Bennani, Mohamed; Mea, Marianna; Todorova, Nadezhda; Karamfilov, Ventzislav; Ten Hoopen, Petra; Cochrane, Guy; L'Haridon, Stephane; Bizsel, Kemal Can; Vezzi, Alessandro; Lauro, Federico M.; Martin, Patrick; Jensen, Rachelle M.; Hinks, Jamie; Gebbels, Susan; Rosselli, Riccardo; De Pascale, Fabio; Schiavon, Riccardo; Dos Santos, Antonina; Villar, Emilie; Pesant, Stephane; Cataletto, Bruno; Malfatti, Francesca; Edirisinghe, Ranjith; Silveira, Jorge A. Herrera; Barbier, Michele; Turk, Valentina; Tinta, Tinkara; Fuller, Wayne J.; Salihoglu, Ilkay; Serakinci, Nedime; Ergoren, Mahmut Cerkez; Bresnan, Eileen; Iriberri, Juan; Nyhus, Paul Anders Fronth; Bente, Edvardsen; Karlsen, Hans Erik; Golyshin, Peter N.; Gasol, Josep M.; Moncheva, Snejana; Dzhembekova, Nina; Johnson, Zackary; Sinigalliano, Christopher David; Gidley, Maribeth Louise; Zingone, Adriana; Danovaro, Roberto; Tsiamis, George; Clark, Melody S.; Costa, Ana Cristina; El Bour, Monia; Martins, Ana M.; Collins, R. Eric; Ducluzeau, Anne-lise; Martinez, Jonathan; Costello, Mark J.; Amaral-zettler, Linda A.; Gilbert, Jack A.; Davies, Neil; Field, Dawn; Gloeckner, Frank Oliver.
Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world's oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.
Tipo: Text
Palavras-chave: Ocean sampling day; OSD; Biodiversity; Genomics; Health Index; Bacteria; Microorganism; Metagenomics; Marine; Micro B3; Standards.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00371/48262/48647.pdf

Interaction de la bactérie Vibrio harveyi avec son hôte, l'hormeau Haliotis tuberculata : approches physiologiques, cellulaires et moléculaires
Provedor de dados: 5
Autores: Travers, Marie-agnes.
Abalone aquaculture only recently developed in western Europe, but already it has to face numerous pathologies. Since 1998, the natural and farm stocks of Haliotis tuberculata suffer regularly high mortalities, mainly due to the pathogenic bacterium, Vibrio harveyi. The vibriosis of the European abalone, in particular its macroscopic signs, and the conditions necessary for its development have been studied in detail by field and laboratory experiments. We have been able to show the importance of the interplay between water temperature (&gt;17°C), animal physiology (reproduction period) and a pathogenic strain (harbouring the plasmid pVCR1) for the development of this abalone disease. A limited localisation study of the abalone target tissues (gills, muscle...
Tipo: Text
Palavras-chave: Ormeau; Haliotis tuberculata; Bactéries; Vibrio harveyi; Vibriose; Température; Immunité; Reproduction; Hémocytes; MAP Kinases; Plasmide; Virulence; GFP; Abalone; Haliotis tuberculata; Bacteria; Vibrio harveyi; Vibriosis; Temperature; Immunity; Reproduction; Haemocytes; MAP Kinases; Plasmid; Virulence; GFP.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00176/28729/27196.pdf

Role des bacteries phototrophes anoxygeniques dans une partie stratifiee d'une lagune tropicale (Lagune Ebrie, Cote d'Ivoire)
Provedor de dados: 5
Autores: Caumette, P.
In the deepest parts of the estuarine region of Ebrie Lagoon (Abidjan Africa, (5 degree N, 4 degree W) stratified conditions have been observed during the rainy season (May to Nov.), owing to salinity differences between oxic and anoxic layers. At the interface, 3-4 m depth, phototrophic bacteria (Chromatium, Chlorobium and Pelodictyon ) developed, forming a brown layer due to their different pigments. Their production has been estimated at 41% of the total photosynthetic production during high stratification (Sep. 1981) and their biomass was used by the copepod population for 40 to 60% of its diet. During the dry season, the water column became homogeneous and oxygen was detected to 6m depth. The brown layer of phototrophic bacteria disappeared, as well...
Tipo: Text
Palavras-chave: Chlorobium; Chromatium; Primary production; Photosynthesis; Estuaries; Seasonal variations; Plankton; Stratification; Vertical distribution; Bacteria; Coastal lagoons; Trophic relationships; Biomass.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-957.pdf

Depuration centre management. Course notes
Provedor de dados: 5
Autores: O'Carroll, Terence; Boulter, Mark; Seaver, Mary; Masson, Daniel; O'Driscoll, Michael; Roden, Cillian.
The purpose of this course is to train and familiarise people, who are going to build, have just built or are about to modernise a purification plant, in the latest technology available to the industry and also to train people so that they can effectively run a depuration centre.  ln general the course aims to :  - Give participants practical experience of different systems prior to committing themselves to a particular system.  - Enable participants to compare systems.  - Provide a minimum training qualification to run an establishment which is essentially a facility to guarantee health and safety of products to the consumer.  - Provide an overview from bacteria testing to plant lay out etc.  The structure of the course is in modules, morning and...
Tipo: Text
Palavras-chave: Shellfish depuration; Purification; Bacteria; Viruses.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00140/25082/23192.pdf

A dual symbiosis shared by two mussel species, Bathymodiolus azoricus and Bathymodiolus puteoserpentis (Bivalvia : Mytilidae), from hydrothermal vents along the northern Mid-Atlantic Ridge
Provedor de dados: 5
Autores: Duperron, Sebastien; Bergin, C; Zielinski, F; Blazejak, A; Pernthaler, A; Mckiness, Z; Dechaine, E; Cavanaugh, C; Dubilier, Nicole.
Bathymodiolus azoricus and Bathymodiolus puteoserpentis are symbiont-bearing mussels that dominate hydrothermal vent sites along the northern Mid-Atlantic Ridge (MAR). Both species live in symbiosis with two physiologically and phylogenetically distinct Gammaproteobacteria: a sulfur-oxidizing chemoautotroph and a methane-oxidizer. A detailed analysis of mussels collected from four MAR vent sites (Menez Gwen, Lucky Strike, Rainbow, and Logatchev) using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization (FISH) showed that the two mussel species share highly similar to identical symbiont phylotypes. FISH observations of symbiont distribution and relative abundances showed no obvious differences between the two host species. In...
Tipo: Text
Palavras-chave: Bacteria; Phylogeny; Fluorescence in situ hybridization (FISH); Methane oxidizer; Sulfur oxidizer; 16S rRNA; Endosymbiosis.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1864.pdf

Observation on the bacteriology of fronts : some problems of methodology
Provedor de dados: 5
Autores: Floodgate, G.d.; Lochte, K.; Egan, B.
The data obtained in surveys to investigate the bacteriological aspects of the biology of two contrasting frontal systems has shown that the Inherent homeostasis and the complexity of the environment, together with the Imprecision of the data, lead to biomass values that are difficult to interpret in terms of the physical factors governing the formation of fronts. Although biochemical measurements offer some promise of providing more reliable and less confusing information, the oeed to develop high precision methods that can be made very frequently is emphasised.
Tipo: Text
Palavras-chave: Bacteria; Discontinuities; Fronts; Methodology.
Ano: 1982
URL: http://archimer.ifremer.fr/doc/00306/41742/40953.pdf

Phased oscillations in cell numbers and nitrate in batch cultures of Alexandrium tamarense (Dinophyceae)
Provedor de dados: 5
Autores: Collos, Yves; Hadjadji, Imene; Plisson, Benoit; Cecchi, Philippe; Laabir, Mohamed; Bechemin, Christian; Masseret, Estelle.
Alexandrium tamarense (M. Lebour) Balech strains isolated in spring 2007 from a single bloom in Thau lagoon have been grown in nonaxenic artificial media. For three strains showing large oscillations in biomass (crashes followed by recoveries) on a scale of several days, a significant relationship was observed between changes in cell densities (as in vivo fluorescence) and changes in nitrate concentrations. Increases in cell densities were accompanied by decreases in nitrate, while decreases in cell densities corresponded to increases in nitrate, presumably due to nitrification. Net increases in nitrate could reach up to 15 mu mol N . L(-1) . d(-1) indicating a very active nitrifying archaeal/bacterial population. However, following population crashes,...
Tipo: Text
Palavras-chave: Alexandrium tamarense; Archaea; Bacteria; Decay; Growth; Nitrate; Nitrification; Oscillations.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00049/16046/13585.pdf

Interactions microalgues-bactéries en système expérimental bispécifique : effets sur la croissance de Dunaliella sp.
Provedor de dados: 5
Autores: Le Chevanton, Myriam.
The use of algae-bacteria mixed culture has been suggested to improve performance of industrial microalgae culture. The present study aims to investigate impact of selected bacteria on Dunaliella sp. growth. 97 bacteria strains were isolated from a variety of marine microalgae cultures. The diversity of strains revealed members of α-and γ-proteobacteria, Cytophaga-Flavobacterium-Bacteroides and grampositive. A two-phase approach was then implemented to select bacteria that improve Dunaliella sp. growth, using an original high-throughput experimental device. Although a majority of bacteria induced performance reduction for Dunaliella sp., some resulted in significant improvements. In nitrogenlimited batch culture, the Alteromonas sp. bacteria demonstrated a...
Tipo: Text
Palavras-chave: Interactions; Microalgues; Bactéries; Dunaliella; Axénie; Interactions; Microalgae; Bacteria; Dunaliella; Axenic.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00187/29839/28291.pdf

A test of community assembly rules using foliar endophytes from a tropical forest canopy
Provedor de dados: 5
Autores: Donald, Julian; Roy, Mélanie; Suescun, Uxue; Iribar, Amaia; Manzi, Sophie; Péllissier, Léonie; Gaucher, Philippe; Chave, Jérôme.
1.Community assembly theory assumes that ecological communities are spatially delimited into patches. Within these patches, coexistence results from environmental filtering, competition, and immigration. Truly delineated communities exist in laboratory studies of microbial cultures in Petri dishes, yet empirical tests conducted in continuous environments often use patches defined by convention as opposed to realised boundaries. 2.Here we perform a test of ecological community assembly rules using foliar endophyte communities from a tropical rainforest, where leaves are considered as patches for both fungal and bacterial communities. We determined the diversity of fungal and bacterial endophytes using environmental DNA sequencing of 365 top‐canopy leaves,...
Tipo: Text
Palavras-chave: Bacteria; Distribution; French Guiana; Fungi; Metabarcoding; Plant–pathogen interactions; Rainforest.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00606/71808/70296.pdf

Recherche et identification de bactéries pathogènes au sein d'une écloserie nurserie d'huître creuse, Crassostrea gigas, en relation avec la mortalité estivale du naissain
Provedor de dados: 5
Autores: Waechter, Magali.
Summer mortality has been observed on oyster spat Crassostrea gigas since 1991 on french coasts. In our study, we deseribed symptoms observed during the mortality, especially adductor muscle weakness and we isolated strains on Zobell medium and TCBS from moribund spat. Strains isolated during a high mortality (60 %) were selected by experimental pathology on spat. Inoculation by bath led us to select a pathogenic strain (TNEMF6) for oyster spat. Phenotypic identification, phylogenetic study on 16S rDNA and quantitative DNA/DNA hybridisation showed that this strain belongs to Vibrio splendidus biovar II. Phenotyping and ribotyping with strains isolated during the study showed that only TNNIII7 and TNMB5 are similar to the pathogenic strain TNEMF6....
Tipo: Text
Palavras-chave: Mortalité estivale; Naissain; Crassostrea gigas; Ecloserie; Nursery; France; Bactériologie; Bactérie; Vibrio; Vibrio splendidus biovar II; Summer mortality; Oyster spat; Crassostrea gigas; Hatchery; Nursery; France; Bacteriology; Bacteria; Vibrio; Vibrio splendidus biovar II.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00211/32226/30682.pdf

L'ionisation des produits de la pêche
Provedor de dados: 5
Autores: Kodo, Jean-louis.
Fishes and fishery products are seafoods which have a nutritive value with a high proteins rate. Fishery products ionization has four steps: the radappertization (sterilization of the seafood), the radicidation (destruction of the pathogen germs), the radurization (food conservation and microbial flora reduction), the desinsectization (insects and parasites elimination). Fishery products quality control is very important
Tipo: Text
Palavras-chave: Legislation; Microbial contamination; Marine fish; Disinfection; Pathogens; Sterilization; Irradiation; Ionizing radiation; Fish inspection regulations; Quality control; Seafood; Processed fishery products; Bacteria.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/rapport-649.pdf

Caractérisation de la virulence d'une souche de Vibrio aestuarianus, pathogène de l'huître Crassostrea gigas
Provedor de dados: 5
Autores: Labreuche, Yannick.
In France. shellfish production is a traditional. well-established industry. mainly based upon commercial farming of the Pacifie oyster. Crassostrea gigas. Since the 1980's, annual mass mortalities of C. gigas have been reported during summer. Several studies on this subject have demonstrated that these mortality outbreaks resulted from complex interactions between the physiologieal andJor genetic status of the oysters. environmental factors, and one or more infectious agents, among which are bacteria of the genus Vibrio.
Tipo: Text
Palavras-chave: Huître; Crassostrea gigas; Bactéries; Vibrio aestuarlanus; Pathogénicité; Produits extracellulaires; Métalloprotéase; Hémocytes; Interactions hôte/pathogène; Oyster; Crassostrea gigas; Bacteria; Vibrio aestuarianus; Pathogenicity; Extracellular products; Metalloprotease; Hemocytes; Host/pathogen interactions.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00034/14561/11859.pdf

La qualité des eaux dans l'Estuaire de la Gironde. 1- Traitement des données. 2 - Synthèse
Provedor de dados: 5
Autores: Maurice, Laurence.
The main problem of the Girond estuary is a pollution by the cadmium. During this last ten years, the good quality of the water is proved by the analysis of the main physico - chemical and biochemical parameters and bacteria. But since 1989, straight to the Blayais power station, the BOD5 varied between 2 and 3,5 mg.l-1 and a diminution of the dissolved oxygen has been observed; it's probably caused by the accumulation of biodegradable organic matter in the maximum turbidity zone during years characterized by low river flows. For average river flow, urban discharges takes part for 20 % in total fluxes of BD05; in 1991, this part attempts 35 %. During the last 7 years, the pluriannual evolution of the nitrate concentrations measured in the Garonne river...
Tipo: Text
Palavras-chave: Oxygène; Sels nutritifs; Matière organique; Bactéries; Apports; Estuaire Gironde; Oxygen; Nutrients; Organic matter; Bacteria; Fluxes; Gironde estuary.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00037/14855/12178.pdf

Shewanella profunda sp nov., isolated from deep marine sediment of the Nankai Trough
Provedor de dados: 5
Autores: Toffin, Laurent; Bidault, Adeline; Pignet, Patricia; Tindall, Brian; Slobodkin, Alexander; Kato, Chiaki; Prieur, Daniel.
A novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain LT13a(T)) was isolated from a deep sediment layer in the Nankai Trough (Leg 190, Ocean Drilling Program) off the coast of Japan. This organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. Oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. Maximal growth rates were observed at a hydrostatic pressure of 10 MPa. Hydrostatic pressure for growth was in the range 0.1-50 MPa. Predominant cellular fatty acids were 16:1 omega7c, 15: 0 iso, 16: 0 and 13: 0 iso. The G + C content of the DNA was 44.9 mol%. On the...
Tipo: Text
Palavras-chave: Phylogeny; Bacteria; Microbiology; Deep sediment layer.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-590.pdf

Dual symbiosis in a Bathymodiolus sp mussel from a methane seep on the gabon continental margin (southeast Atlantic): 16S rRNA phylogeny and distribution of the symbionts in gills
Provedor de dados: 5
Autores: Duperron, Sebastien; Nadalig, Thierry; Caprais, Jean-claude; Sibuet, Myriam; Fiala Medioni, Aline; Amann, Rudolf; Dubilier, Nicole.
Deep-sea mussels of the genus Bathymodiolus (Bivalvia: Mytilidae) harbor symbiotic bacteria in their gills and are among the dominant invertebrate species at cold seeps and hydrothermal vents. An undescribed Bathymodiolus species was collected at a depth of 3,150 m in a newly discovered cold seep area on the southeast Atlantic margin, close to the Zaire channel. Transmission electron microscopy, comparative 16S rRNA analysis, and fluorescence in situ hybridization indicated that this Bathymodiolus sp. lives in a dual symbiosis with sulfide- and methane-oxidizing bacteria. A distinct distribution pattern of the symbiotic bacteria in the gill epithelium was observed, with the thiotrophic symbiont dominating the apical region and the methanotrophic symbiont...
Tipo: Text
Palavras-chave: Gabon; Southeast Atlantic; Methane seep; Hydrothermal vent; Deep sea; RRNA analysis; Methane concentration; Gill epithelium; Symbiosis; Bacteria; Mytilidae.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1266.pdf

Metabolism of CO and CH sub(4) by nitrifiers and the determination of the nitrification rate.
Provedor de dados: 5
Autores: Morita, R; Jones, R.
The nitrifying bacteria were found to survive 24 weeks in the absence of ammonium without decreasing their number of cell size. Because H sub(2), CO, and CH sub(4) are present in the marine environment, these substrates were investigated as a possible source of the energy of maintenance for the nitrifying bacteria. super(14)CO and super(14)CH sub(4) were found to be oxidized by the nitrifiers. N-serve was found to inhibit the oxidation of CO. Using the nitrifiers' ability to oxide CO, a method for the determination of the nitrification rate was developed. The ability of nitrifiers to oxidize CO may play a significant role in the cycling of CO sub(2) in the marine environment. Whether CO and CH sub(4) oxidation play a role in the survival of nitrifiers in...
Tipo: Text
Palavras-chave: Methane; Carbon dioxide; Survival; Bacteria; Nitrification; Nitrogen cycle; Oxidation; Carbon cycle; Biogeochemistry; Metabolism.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-975.pdf

Plasmid-associated phenathrene degradation by Chesapeake Bay sediment bacteria.
Provedor de dados: 5
Autores: Okpokwasili, G; Somerville, C; Grimes, D; Colwell, R.
A differential, phenanthrene-enrichment agar plating technique was used to isolate phenanthrene-degrading bacteria from phenanthrene-enriched Chesapeake Bay sediment. One of the isolates, a yellow pigmented, slime-producing, Gram-negative rod identified as Flavobacterium sp. has been studied in detail. It has been found to carry a single plasmid with a mass of about 34 megadaltons. Results of hydrocarbon adherence tests showed that the organism adhered only minimally to n-octane and n-hexadecane, but emulsified cyclohexylbenzene and 1,2,3,4,-tetrahydronaphthalene (tetralin). Curing of the plasmid with 3 mu g/ml novobiocin resulted in loss of phenanthrene clearing ability. Ability to degrade phenanthrene was confirmed using super(14)C-labeled phenanthrene.
Tipo: Text
Palavras-chave: Flavobacterium; Microbiological culture; Culture media; Bacteria; Sediment pollution; Biodegradation; Bacteria collecting devices.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1014.pdf

Kinetics of tidal resuspension of microbiota: Testing the effects of sediment cohesiveness and bioturbation using flume experiments
Provedor de dados: 5
Autores: Blanchard, Gérard F; Sauriau, Pg; Cariou-le Gall, Valerie; Gouleau, D; Garet, Mj; Olivier, F.
Resuspension of the top few sediment layers of tidal mud flats is known to enhance planktonic biomass of microbiota (benthic diatoms and bacteria). This process is mainly controlled by tidal shear stress and cohesiveness of mud, and is also influenced by bioturbation activities. Laboratory experiments in a race track flume were performed to test the interactive effects of these factors on both the critical entrainment and resuspension kinetics of microbiota from silt-clay sediments from the Marennes-Oleron Bay, France. The marine snail Hydrobia ulvae was used to mimic surface bioturbation activities. As expected, the kinetics of microbial resuspension versus shear stress were largely controlled by the cohesiveness of silt-clay sediments. However, our...
Tipo: Text
Palavras-chave: Microphytobenthos; Bacteria; Resuspension; Bioturbation; Hydrobia ulvae; Shear velocity; Sediment cohesiveness; Flume experiment.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00337/44789/44480.pdf

Evolution de la communaute bacterienne heterotrophe de l'eau de mer lors d'une experience d'aquaculture de crevettes peneides en systemes clos
Provedor de dados: 5
Autores: Sohier, L; Bianchi, M.
The quantitative and qualitative evolution of the heterotrophic bacterial community of sea water was studied throughout an experimental rearing of Penaeus japonicus in closed systems. Cluster analysis was realised on 417 strains coming from water sampled at different moments of the experiment. This collection includes 40 strains coming from a mussel used for diet and 40 strains coming from the digestive tract of one prawn after six months rearing. Quantitative and qualitative studies have both shown a similar evolution of heterotrophic microflora whatever the initial animal load was. The dendrogram obtained led to a description of characteristics of the various populations constituting the heterotrophic bacterial community of the water environment...
Tipo: Text
Palavras-chave: Penaeus japonicus; Malacostraca; Bacteria; Recirculating systems; Numerical taxonomy; Temporal variations; Microorganisms; Shrimp culture; Heterotrophic organisms.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-972.pdf

Desulfurobacterium atlanticum sp nov., Desulfurobacterium pacificum sp nov and Thermovibrio guaymasensis sp nov., three thermophilic members of the Desulfurobacteriaceae fam. nov., a deep branching lineage within the Bacteria
Provedor de dados: 5
Autores: L' Haridon, S; Reysenbach, A.l.; Tindall, B. J.; Schonheit, P; Banta, A; Johnsen, U; Schumann, P; Gambacorta, A; Stackebrandt, E; Jeanthon, C.
Three thermophilic, anaerobic, strictly chemolithoautotrophic, sulphur- and/or thiosulphate-reducing bacteria, designated SL17(T), SL19(T) and SL22(T), were isolated from deep-sea hydrothermal samples collected at 13 degrees N (East Pacific Rise), Guaymas Basin (Gulf of California) and 23 degrees N (Mid-Atlantic Ridge), respectively. These strains differed in their morphology, temperature range and optimum for growth, energy substrates and 16S rRNA gene sequences. The G + C content of the genomic DNA was 41 mol% (SL22(T)), 42 mol% (SL17(T)) and 46 mol% (SL19(T)). Comparative analysis of phenotypic and phylogenetic traits indicated that strains SL17(T) and SL22(T) represented two novel species of the genus Desulfurobacterium and that strain SL19(T) should...
Tipo: Text
Palavras-chave: Hydrothermal environment; Phylogeny; Thermophiles; Bacteria.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2174.pdf

Etude des liens trophiques au sein d'une communaute bacterienne selectionnee a partir d'un sediment marin sur le cyclohexene-4 dicarboximide-1,2 (THPI)
Provedor de dados: 5
Autores: Terrom, G; Gil, G; Lepetit, J.
A bacterial community has been selected from a marine sediment on 4-cyclohexene-1,2 dicarboximide or THPI (fungicide). This community consists of nine strains. The "principal" strain, Photobacterium mandapamensis , degrades THPI by different metabolic pathways many of which require co-oxidative sequences. The other eight "auxiliary" strains occur at different states along the trophic chain formed by the "principal" strain, so increasing velocity and intensity of the THPI biodegradation.
Tipo: Text
Palavras-chave: Bacteria; Marine pollution; Microorganisms; Metabolism; Fungicides; Biodegradation; Sediment pollution; Trophic relationships.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1021.pdf

Effects of petroleum biodegradation products on phytoplankton growth.
Provedor de dados: 5
Autores: Goutx, H; Berland, B; Leveau, M; Bertrand, J.
The effects of products resulting from petroleum biodegradation in continuous culture, have been studied on the growth of two marine algae. Sugars, lipids and amino acids are the main components resulting from petroleum degradation in the bacteria used medium. Their concentrations are respectively 600 mg.l super(-1), 46 mg.l super(-1) and 74.2 mu M.l super(-1). The algae growth is inhibited when products are enough concentrated; it is stimulated by low concentration. Among these products, lipids are more toxic than others. The two species show different sensitivity towards the used medium. Prorocentrum micans growth is more inhibited than that of Phaeodactylum tricornutum .
Tipo: Text
Palavras-chave: Bacteria; Phytoplankton; Toxicity; Oil pollution; Crude oil; Biodegradation.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1017.pdf

Interspecific differences in the effect of fish on marine microbial plankton
Provedor de dados: 5
Autores: Villeger, Sebastien; Fouilland, Eric; Argenty, Jeremy; Bouvier, Corinne; Carre, Claire; Bouvier, Thierry.
The productivity of most marine ecosystems is limited by the availability of dissolved nitrogen (N) and phosphorus (P). Nutrient recycling is therefore a key process for ecosystem functioning. Fish recycle nutrients through the excretion of ammonia and phosphate and can influence the abundance and community structure of primary producers such as phytoplankton. However, the effect of fish on other plankton compartments, and whether all fish species have similar effects, is largely unknown. We used a tank experiment to test how 2 Mediterranean fish species, gilthead seabream Sparus aurata and golden mullet Chelon auratus, with distinctly different N and P excretion rates, can affect the abundance and community structure of 3 plankton compartments:...
Tipo: Text
Palavras-chave: Marine fish; Nutrient excretion; Protozooplankton; Phytoplankton; Bacteria.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00584/69659/67585.pdf

Effect of xanthan on the growth of sulphate-reduction bacteria in marine sediments.
Provedor de dados: 5
Autores: Battersby, N; Stewart, D; Sharma, A.
Chemical additives are used extensively in the offshore oil industry. For secondary oil recovery, the biopolymer xanthan has been recommended as a viscosifying agent but its use may provoke microbial problems. The effect of adding xanthan to marine sediments on the growth and metabolic activity of sulphate-reducing bacteria (SRB) was studied. In all instances, its addition resulted in an increasing in SRB numbers of up to 250-fold. This was accompanied by a marked increase in the electron transport activity of the sediments when these were incubated under either aerobic or anaerobic conditions. The products formed as a result of the biodegradation of the polymer by indigenous heterotrophic bacteria in these sediments appear to have acted as suitable...
Tipo: Text
Palavras-chave: Bacteria; Growth; Additives; Pollution effects; Oil recovery; Biodegradation; Polymers; Sediment pollution; Sulfate reduction.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1015.pdf

Influence of CH4 and H2S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel Bathymodiolus azoricus
Provedor de dados: 5
Autores: Riou, V.; Halary, S.; Duperron, S.; Bouillon, S.; Elskens, M.; Bettencourt, R.; Santos, R. S.; Dehairs, F.; Colaco, A.
High densities of mussels of the genus Bathymodiolus are present at hydrothermal vents of the Mid-Atlantic Ridge. It was previously proposed that the chemistry at vent sites would affect their sulphide- and methane-oxidizing endosymbionts' abundance. In this study, we confirmed the latter assumption using fluorescence in situ hybridization on Bathymodiolus azoricus specimens maintained in a controlled laboratory environment at atmospheric pressure with one, both or none of the chemical substrates. A high level of symbiosis plasticity was observed, methane-oxidizers occupying between 4 and 39% of total bacterial area and both symbionts developing according to the presence or absence of their substrates. Using (HCO3-)-C-13 in the presence of sulphide, or...
Tipo: Text
Palavras-chave: Mid-atlantic ridge; Hydrocarbon-seep mussel; Deep-sea mussels; Hydrothermal-vent; Puteoserpentis bivalvia; Mytilidae; Methane; Endosymbionts; Bacteria; Metabolism.
Ano: 2008
URL: https://archimer.ifremer.fr/doc/00207/31865/30271.pdf

Factors affecting bacterial biomass and growth in the Duplin River estuary and coastal Atlantic Ocean.
Provedor de dados: 5
Autores: Fallon, R; Newell, S; Sherr, B; Sheer, E.
Bacterial numbers and growth rates were measured in coastal, planktonic systems of the Western Atlantic ocean. Seasonal responses in growth rates of the bacterial assemblage were present at some sites, but these may be related to seasonal changes in substrate supply rather than to changes in temperature. Bacterial growth rates tended to decline with increasing distance from shore. There was evidence for predator-prey population cycles in the bacteria and &lt; 17 mu m protozoa in the Duplin River Estuary which may indicate that the bacterial assemblage serves as prey for protozoan populations. In summer, a significant negative correlation between mu (instantaneous growth rate) and cell numbers in the bacterial assemblage indicated that substrate limitation...
Tipo: Text
Palavras-chave: Protozoa; Plankton; Bacteria; Predators; Grazing; Temperature effects; Seasonal variations; Limiting factors; Interspecific relationships; Nutrients (mineral); Growth.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-955.pdf

Fecal contamination in coastal areas: An engineering approach
Provedor de dados: 5
Autores: Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick.
Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These...
Tipo: Text
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf

Unlike the Escherichia coli counterpart, archaeal RNase HII cannot process ribose monophosphate abasic sites and oxidized ribonucleotides embedded in DNA
Provedor de dados: 5
Autores: Malfatti, Matilde Clarissa; Henneke, Ghislaine; Balachander, Sathya; Koh, Kyung Duk; Newnam, Gary; Uehara, Ryo; Crouch, Robert J.; Storici, Francesca; Tell, Gianluca.
The presence of ribonucleoside monophosphates (rNMPs) in nuclear DNA decreases genome stability. To ensure survival despite rNMP insertions, cells have evolved a complex network of DNA repair mechanisms, in which the ribonucleotide excision repair pathway, initiated by type 2 ribonuclease H (RNase HII/2), plays a major role. We recently demonstrated that eukaryotic RNase H2 cannot repair damaged, that is, ribose monophosphate abasic (both apurinic or apyrimidinic) site (rAP) or oxidized rNMP embedded in DNA. Currently, it remains unclear why RNase H2 is unable to repair these modified nucleic acids having either only a sugar moiety or an oxidized base. Here, we compared the endoribonuclease specificity of the RNase HII enzymes from the archaeon Pyrococcus...
Tipo: Text
Palavras-chave: Ribonuclease; Bacteria; Escherichia coli (E coli); Archaea; Oxidative stress; Abasic-ribose; Oxidized-ribonucleotides; Pyrococcus abyssi; Type 2 RNase H.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00506/61796/65801.pdf

Diversité phylogénétique et fonctionnelle des communautés microbiennes incultivées des sédiments marins de la marge de Sonora, Bassin de Guaymas (Golfe de Californie)
Provedor de dados: 5
Autores: Vigneron, Adrien.
At continental margins, and more particularly in cold seep areas, microbial and animal communities were locally detected at the surface of the sediments. These communities grow using reduced chemical compounds (H2S, Methane, COZ ...) contained in the percolated cold fluids and produced by both geological and microbial processes. ln order to study microbial community diversity in these ecosystems and their role in the environment as well as to understand the environmental factors influencing the distribution and ecophysiology of these communities, surface (0-20 cmbsf) but also deeper (&lt;9 mbsf) sediments were collected at the Sonora Margin. Microbial communities have been studied using various molecular, cultural and microscopy approaches. This research...
Tipo: Text
Palavras-chave: Fluides froids; Marge de Sonora; Bassin de Guaymas; Diversité moléculaire; Communautés microbiennes; Archaea; Bacteria; Méthanogènes; Methanogens; Microbial communities; Molecular diversity; Guaymas Basin; Sonora Margin; ANME; SRb; Methane; Sediments; Cold seeps.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00498/60956/64359.pdf

An abyssal mobilome: Viruses, plasmids and vesicles from deep-sea hydrothermal vents
Provedor de dados: 5
Autores: Lossouarn, Julien; Dupont, Samuel; Gorlas, Aurore; Mercier, Coraline; Bienvenu, Nadege; Marguet, Evelyne; Forterre, Patrick; Geslin, Claire.
Mobile genetic elements (MGEs) such as viruses, plasmids, vesicles, gene transfer agents (GTAs), transposons and transpovirions, which collectively represent the mobilome, interact with cellular organisms from all three domains of life, including those thriving in the most extreme environments. While efforts have been made to better understand deep-sea vent microbial ecology, our knowledge of the mobilome associated with prokaryotes inhabiting deep-sea hydrothermal vents remains limited. Here we focus on the abyssal mobilome by reviewing accumulating data on viruses, plasmids and vesicles associated with thermophilic and hyperthermophilic Bacteria and Archaea present in deep-sea hydrothermal vents.
Tipo: Text
Palavras-chave: Deep-sea hydrothermal vent; Bacteria; Archaea; (Hyper-)thermophiles; Mobilome.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00260/37145/36731.pdf

Stress factors resulting from the Arctic vernal sea-ice melt : Impact on the viability of bacterial communities associated with sympagic algae
Provedor de dados: 5
Autores: Amiraux, Rémi; Burot, Christopher; Bonin, Patricia; Massé, Guillaume; Guasco, Sophie; Babin, Marcel; Vaultier, Frédéric; Rontani, Jean-françois.
During sea-ice melt in the Arctic, primary production by sympagic (sea-ice) algae can be exported efficiently to the seabed if sinking rates are rapid and activities of associated heterotrophic bacteria are limited. Salinity stress due to melting ice has been suggested to account for such low bacterial activity. We further tested this hypothesis by analyzing samples of sea ice and sinking particles collected from May 18 to June 29, 2016, in western Baffin Bay as part of the Green Edge project. We applied a method not previously used in polar regions—quantitative PCR coupled to the propidium monoazide DNA-binding method—to evaluate the viability of bacteria associated with sympagic and sinking algae. We also measured cis-trans isomerase activity, known to...
Tipo: Text
Palavras-chave: Bacteria; Sea ice; Sympagic algae; Ice biota; Viability; Stress factors; Salinity; Bactericidal free fatty acids; Carbon export.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00681/79329/81840.pdf

Adaptive features of a bacteriolytic enzyme from the style of the mussel Choromytilus meridionalis in response to environmental fluctuations.
Provedor de dados: 5
Autores: Seiderer, L; Robb, F.
The extracellular digestive apparatus of bivalve molluscs consists of a crystalline style containing diverse degradative enzymes which are released during rotation and dissolution of the style. This paper reviews the occurrence of a temperature-regulated bacteriolytic enzyme in the black mussel Choromytilus meridionalis , which is an inhabitant of coastal reefs on the south and south west coast of South Africa. This agent is unable to lyse Micrococcus luteus or Escherichia coli , both of which are susceptible to lysis by a true lysozyme. It appears to have a restricted spectrum of activity which includes both free-living and kelp-associated gram-negative bacteria. In addition, the level of activity varies dramatically according to the environmental water...
Tipo: Text
Palavras-chave: Choromytilus meridionalis; Bivalvia; Digestive system; Bacteria; Upwelling; Environmental effects; Adaptations; Bacteriocides; Marine molluscs; Enzymatic activity.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-991.pdf

Nitrate respiration and nitrification in estuarine sediments.
Provedor de dados: 5
Autores: Herbert, R.
Seasonal data and depth profiles using super(15)N-labelled NO sub(3)u- show that denitrification (77-90% of NO sub(3)u- respired) rather than NO sub(3)u- dissimilation to NH sub(4)u+ was the principal route of nitrate reduction in Kingoodie Bay sediments. Populations of both groups of NO sub(3)u- reducing bacteria were highest in the 0-20 mm horizon in those sediments where highest rates of NO sub(3)u- respiration were recorded (28.56 mu g N.d super(-1) dry wt. sediment super(-1)). Autotrophic nitrification rates shared a marked seasonality with highest rates (0.92 mu g N.d super(-1).g dry wt. sediment super(-1)) occurring during the summer. Maximum populations of autotrophic nitrifying bacteria were also found in the 0-20 mm sediment horizon and these...
Tipo: Text
Palavras-chave: Seasonal variations; Bacteria; Estuaries; Sediment chemistry; Biogeochemistry; Ammonia; Nitrates; Nitrogen cycle.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-971.pdf

Benthic foraminifera from the deep-water Niger delta (gulf of Guinea): Assessing present-day and past activity of hydrate pockmarks
Provedor de dados: 5
Autores: Fontanier, Christophe; Koho, K. A.; Goni-urriza, M. S.; Deflandre, Bruno; Galaup, S.; Ivanovsky, A.; Gayet, Nicolas; Dennielou, Bernard; Gremare, Antoine; Bichon, S.; Gassie, C.; Anschutz, P.; Duran, R.; Reichart, G. J.
We present ecological and isotopic (δ18O and δ13C) data on benthic foraminifera sampled from 4 deep-sea stations in a pockmark field from the deep-water Niger delta (Gulf of Guinea, Equatorial Atlantic Ocean). In addition, a series of sedimentological and (bio)geochemical data are shown to back up foraminiferal observations. All stations are located within 1.2 km of each other, so prevailing oceanographic conditions can be assumed to be similar at each site. Two of the sites (GMMC-01 and GMMC-02) are located in a pockmark (named “pockmark A”) where current methane seepages were recorded by ROV observations. A third station (GMMC-03) is located in the topographic depression interpreted as a collapsed pockmark (named “pockmark B”). The fourth site (GMMC-04)...
Tipo: Text
Palavras-chave: Niger delta; Hydrate pockmark; Foraminifera; Stable isotopes; Bacteria.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00211/32194/30628.pdf

Nutrition experimentale: Problemes methodologiques lies a l'utilisation des microorganismes comme source trophique
Provedor de dados: 5
Autores: Guidi, L.
The present study deals with technical problems encountered under lab conditions in studies of trophic relations between deposit-feeding invertebrates and microorganisms. Using 2 pure cultures of Pseudomonas and Navicula , it was shown that these microorganisms will attach to artificial (glass beads) and natural (sand grains) substrates. Best microbial attachment was observed on sand (up to 4.8 x 10 super(8) cells.g super(-1) dry wt.). The radioactive labelling of the microorganisms depends not only on the form under which the isotope ( super(14)C) is supplied to the cultures, but also on the time and the duration of the labelling. 70% of the isotope was incorporated in a stable manner by the diatoms. The bacteria incorporated, at most, 34% of the...
Tipo: Text
Palavras-chave: Navicula; Pseudomonas; Bacillariophyceae; Algae; Bacteria; Biological attachment; Radioactive tracers; Methodology; Microorganisms; Trophic relationships; Microbiological culture; Zoobenthos; Nutrition.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-987.pdf

Etat des connaissances sur la pathologie chez les mollusques bivalves
Provedor de dados: 5
Autores: Fouche, Dominique.
A short description of the disease causing organisms affecting bivalves is presented in this document, including the diagnosis, the oustanding research and bivalve husbandry. Mollusc populations may be safe from disease epizootics if an early prevention of disease is coupled with a strict regulatory system on transfer (according to what happens in agriculture). The species to culture may be thoroughly selected and risky populations moved. Imported stocks are strictly controlled. The researchcs's target is to improve the diagnosis tools and try to find resistant mollusc strains. Epidemiological surveys are also necessary to control diseases.
Tipo: Text
Palavras-chave: Transfer; Husbandry; Prophylatics; Bivalve; Protozoan; Virus; Bacteria; Metazoan; Transfert; Zootechnie; Prophylaxie; Bivalve; Protozoaire; Virus; Bactérie; Métazoaire.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/rapport-1923.pdf

Effects of environmental conditions on the sessile existence of an estuarine sediment bacterium.
Provedor de dados: 5
Autores: Geesey, G; Salas, S; Mittelman, M.
A submerged glass coverslip technique was developed to determine dissolved organic nutrient uptake by adherent cells of a sediment isolate of Enterobacter cloacae . Cells which colonized the coverslips in swirling culture flasks during anaerobic growth remained firmly attached during manipulations employed to determine uptake of radiolabeled glucose by the adherent population. The attached cells were capable of a more rapid rate of glucose uptake than free cells. Lineweaver-Burk plots demonstrated different glucose uptake kinetics for the 2 cell populations. The data suggest that physiological changes occur in cells soon after they become attached to surfaces. These changes appear to enhance the metabolic activity of the adherent population.
Tipo: Text
Palavras-chave: Enterobacter cloacae; Adhesion; Glucose; Environmental effects; Bacteria; Sediments; Estuaries; Sessile species; Culture media; Metabolism; Dissolved oxygen; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-967.pdf

Exploration des communautés virales thermophiles dans les écosystèmes chauds des Terres australes et antarctiques françaises
Provedor de dados: 5
Autores: Parikka, Kaarle Joonas.
Viruses thrive in all types of ecosystems where life is found. They represent the most abundant biological entity of our biosphere. Though several studies have been conducted on viral abundance and dynamics in mesophilic aquatic ecosystems, these aspects remain largely unexplored in extremophilic environments, such as hot springs. In this study, prokaryotic and associated viral communities of the French Southern and Antarctic Lands hot springs were explored. First, prokaryotic cells and Virus-like particles (VLP) were enumerated in several terrestrial and inshore hot springs. The results reveal an abundance of 105 - 106 particles/ml in both types of hot springs studied. The virus-to-prokaryote ratios (VPR) were generally low, confirming thus actual...
Tipo: Text
Palavras-chave: Bactérie; Archée; Induction virale; Lysogénie; Source chaude; Abondance; Bacteria; Archaea; Phage; Thermophile; Viral induction; Lysogeny; Hot spring; Abundance.
Ano: 2013
URL: https://archimer.ifremer.fr/doc/00609/72139/70891.pdf

Impact of the Phaeocystis globosa spring bloom on the intertidal benthic compartment in the eastern English Channel: A synthesis
Provedor de dados: 5
Autores: Spilmont, Nicolas; Denis, Lucie; Artigas, Luis Felipe; Caloin, Frederic; Courcot, Lucie; Creach, Anne; Desroy, Nicolas; Gevaert, Francois; Hacquebart, Pascal; Hubas, Cedric; Janquin, Marie-andree; Lemoine, Yves; Luczak, Christophe; Migne, Aline; Rauch, Mathieu; Davoult, Dominique.
From 1999 to 2005, studies carried out in the frame of regional and national French programs aimed to determine whether the Phaeocystis globosa bloom affected the intertidal benthic communities of the French coast of the eastern English Channel in terms of composition and/or functioning. Study sites were chosen to cover most of the typical shore types encountered on this coast (a rocky shore, an exposed sandy beach and a small estuary). Both the presence of active Phaeocystis cells and their degradation product (foam) did have a significant impact on the studied shores. The primary production and growth rates of the kelp Saccharina latissima decreased during the bloom because of a shortage of fight and nutrient for the macroalgae. On sandy sediments, the...
Tipo: Text
Palavras-chave: Macrozoobenthos; Bacteria; Saccharina latissima; Benthic fluxes; Phaeocystis globosa; Intertidal.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6142.pdf

Interactions entre les bacteries et les algues dans une culture continue de phytoplancton naturel soumise aux conditions exterieures
Provedor de dados: 5
Autores: Gauthier, M; Martin, Y; Lelong, P; Breittmayer, V.
During an experimental continuous culture of natural marine phytoplankton in environmental conditions, microbial and biochemical tests performed to study production of antibacterial and antialgal substances by unicellular algae. Some parameters were further monitored to characterize bacteria and phytoplankton growth. The presence of inhibitors of algal origin was studied both in the water and in the algal biomass at different phases of the culture, through bioassays performed with bacteria (marine pseudomonads and vibrios, and telluric strains) and several algal strains. Interpretation of the results is difficult. Two successive phases appeared during the growth of phytoplankton, with two different algal populations. The unsteady population of phase I was...
Tipo: Text
Palavras-chave: Bacteria; Algae; Microbiological culture; Heterotrophic organisms; Aerobic bacteria; Phytoplankton culture; Sea water; Ecological associations.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-984.pdf

Inadequacy of bacterial indicators for assessing elimination rates of viruses from molluscan shellfish
Provedor de dados: 5
Autores: Burkhardt, W; Watkins, W; Rippey, S.
The reported incidence of shellfish-borne illness in the United States increased dramatically during the last decade. Most of the outbreaks are attributed to diseases of viral aetiology. These human health problems result primarily from the ingestion of raw shellfish which have accumulated enteric pathogens from environmental waters or from wet storage facilities. Our studies have demonstrated that hard-shelled clams concentrate different indicator micro-organisms at variable and unpredictable rates during the year. In temperate waters, this has been observed at two abbreviated periods in mid-spring and again in late fall when accumulation rates increased dramatically. Moreover, these rates were not generally coincident for viruses and bacteria. In...
Tipo: Text
Palavras-chave: Mollusca; Intestinal microflora; Phages; Seafood; Public health; Diseases; Marine molluscs; Viruses; Shellfish; Self purification; Bacteria; Indicator species.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1609.pdf

Ecophysiological studies on luminous bacteria associated with marine gastropods.
Provedor de dados: 5
Autores: Ramesh, A; Venugopalan, V.
Information available on the association of luminous bacteria with marine invertebrates is limited. Hence qualitative and quantitative investigations were undertaken with a view to understand the distribution of luminous microflora in relation to the environmental factors and also in the gut of two marine gastropods, Aplysia benedicti Eliot and Bullia tranquebarica (Roding) from Porto Novo (11 degree 29'N, 79 degree 46'E) waters of the East coast of India. The gut of the gastropods harboured maximum number of luminous bacteria when compared to the water and sediment. Qualitative analysis revealed the presence of two luminous bacterial components, viz. Vibrio harveyi and V. fishceri. V. fischeri was not found in association with A. benedicti . The isolates...
Tipo: Text
Palavras-chave: Gastropoda; Vibrio fischeri; Vibrio harveyi; Bioluminescence; Bacteria; Physicochemical properties; Environmental factors; Microorganisms; Luminous organisms; Interspecific relationships; Ecophysiology.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-993.pdf

Heterotrophic Bacteria Show Weak Competition for Nitrogen in Mediterranean Coastal Waters (Thau Lagoon) in Autumn
Provedor de dados: 5
Autores: Trottet, Aurore; Leboulanger, Christophe; Vidussi, Francesca; Pete, Romain; Bouvy, Marc; Fouilland, Eric.
The importance of heterotrophic bacteria relative to phytoplankton in the uptake of ammonium and nitrate was studied in Mediterranean coastal waters (Thau Lagoon) during autumn, when the Mediterranean Sea received the greatest allochthonous nutrient loads. Specific inhibitors and size-fractionation methods were used in combination with isotopic N-15 tracers. NO3 (-) and NH4 (+) uptake was dominated by phytoplankton (60 % on average) during the study period, which included a flood event. Despite lower biomass specific NH4 (+) and NO3 uptake rates, free-living heterotrophic bacteria contributed significantly (&gt; 30 %) to total microbial NH4 (+) and NO3 (-) uptake rates in low chlorophyll waters. Under these conditions, heterotrophic bacteria may be...
Tipo: Text
Palavras-chave: Bacteria; Phytoplankton; Inhibitors; Size fractionation; Nitrogen uptake; Dissolved inorganic nitrogen.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00314/42497/41907.pdf

Fluorescent antibody enumeration of Vibrio cholerae in the marine environment.
Provedor de dados: 5
Autores: Brayton, P; Roszak, D; Palmer, L; Huq, S; Gimes, D.
From microcosm studies, V. cholerae and related potential human pathogens enter a viable but nonculturable state. Direct viable counts by epifluorescent microscopy consistently remain higher than corresponding plate counts. Thus, the assumption that pathogens "die-off" or "decay" in the marine environment must be re-evaluated, since stressed or nutrient-starved cells are unable to grow and be enumerated by standard plate count methods. Indirect immunofluorescent microscopy offers a more sensitive detection system for environment sampling for human pathogens. Field indicate that cultures positive for V. cholerae O1 are also positive by fluorescent antibody staining; however, the reverse was not necessarily true. By adapting epifluorescent techniques for...
Tipo: Text
Palavras-chave: Vibrio cholerae; Bacteria; Experimental research; Marine environment; Pathogenic bacteria; Microbial contamination; Fluorescence microscopy; Bacterial counters; Pollution detection.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1000.pdf

Bacterivory by benthic organisms in sediment : Quantification using 15N-enriched bacteria
Provedor de dados: 5
Autores: Pascal, P.y.; Dupuy, C; Mallet, C; Richard, P; Niquil, N.
The fate of benthic bacterial biomass in benthic food webs is a topic of major importance but poorly described. This paper describes an alternative method for evaluation of bacterial grazing rate by meiofauna and macrofauna using bacteria pre-enriched with stable isotopes. Natural bacteria from the sediment of an intertidal mudflat were cultured in a liquid medium enriched with 15NH4Cl. Cultured bacteria contained 2.9% of 15N and were enriched sufficiently to be used as tracers during grazing experiments. Cultured bacteria presented a biovolume (0.21 &amp;#956;m3) and a percentage of actively respiring bacteria (10%) similar to those found in natural communities. The number of Operational Taxon Units found in cultures fluctuated between 56 and 75% of that...
Tipo: Text
Palavras-chave: Tracer; Stable isotope; Sediment; Grazing; Bacteria.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4296.pdf

Dynamique des populations bactériennes au cours de deux productions expérimentales de phytoplancton marin naturel
Provedor de dados: 5
Autores: Bianchi, M.a.; Martin, Y.p.
Quantitative and qualitative variations of the hetero­trophic bacterial population, during two experimental induced phytoplanktonic blooms are described. When the phytoplanktonic population deceases, the bacte­rial population exhibits a significant increase. The spring microflore is quite different from the fall ones. Microbiological, physiological and biochemical fluctuations indicate qualitative fluctuations. Bacterial strains are identified as "bacterial profiles".
Tipo: Text
Palavras-chave: Plancton; Bactéries; Dynamique; Physiologie; Structure; Plankton; Bacteria; Dynamic; Physiology; Structure.
Ano: 1979
URL: http://archimer.ifremer.fr/doc/00305/41669/40878.pdf

Thermodesulfobacterium hydrogeniphilum sp nov., a thermophilic, chemolithoautotrophic, sulfate-reducing bacterium isolated from a deep-sea hydrothermal vent at Guaymas Basin, and emendation of the genus Thermodesulfobacterium
Provedor de dados: 5
Autores: Jeanthon, Christian; L' Haridon, Ste! Phane; Cueff, Valerie; Banta, Amy; Reysenbach, Anna-louise; Prieur, Daniel.
A thermophillic, non-spore-forming, marine, sulfate-reducing bacterium, strain SL6(T), was isolated from deep-sea hydrothermal sulfides collected at Guaymas Basin. The Gram-negative-staining cells occurred singly or in pairs as small, highly motile rods. The temperature range for growth was 50-80degreesC with an optimum at 75degreesC. The pH range for growth at 70degreesC was 6.3-6.8, with an optimum at 6.5. The NaCl concentration range for growth was 5.55 g l(-1), with an optimum at 30 g l(-1). H-2 and CO2 were the only substrates for growth and sulfate reduction. However, growth was stimulated by several organic compounds. Sulfur, thiosulfate, sulfite, cystine, nitrate and fumarate were not used as electron acceptors. Pyruvate, lactate and malate did not...
Tipo: Text
Palavras-chave: Sulfate reduction; Thermodesulfobacterium; Bacteria; Thermophile; Deep sea hydrothermal vent.
Ano: 2002
URL: https://archimer.ifremer.fr/doc/2002/publication-585.pdf

Blow Your Nose, Shrimp! Unexpectedly Dense Bacterial Communities Occur on the Antennae and Antennules of Hydrothermal Vent Shrimp
Provedor de dados: 5
Autores: Zbinden, Magali; Gallet, Alison; Szafranski, Kamil M.; Machon, Julia; Ravaux, Juliette; Leger, Nelly; Duperron, Sebastien.
In crustaceans, as in other animals, perception of environmental cues is of key importance for a wide range of interactions with the environment and congeners. Chemoreception involves mainly the antennae and antennules, which carry sensilla that detect water-borne chemicals. The functional importance of these as exchange surfaces in the shrimp's sensory perception requires them to remain free of any microorganism and deposit that could impair the fixation of odorant molecules on sensory neurons. We report here the occurrence of an unexpected dense bacterial colonization on surface of the antennae and antennules of four hydrothermal vent shrimp species. Microscopic observation, qPCR and 16S rRNA barcoding reveal the abundance, diversity and taxonomic...
Tipo: Text
Palavras-chave: Hydrothermal; Shrimp; Alvinocarididae; Chemosensory perception; Antennules; Bacteria; Grooming.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00484/59527/62507.pdf

Viral and Bacterial Epibionts in Thermally-Stressed Corals
Provedor de dados: 5
Autores: Nguyen-kim, Hanh; Bouvier, Thierry; Bouvier, Corinne; Ngoc Bui, Van; Le-lan, Huong; Bettarel, Yvan.
The periodic rise in seawater temperature is one of the main environmental determinants of coral bleaching. However, the direct incidence of these episodic thermal anomalies on coral-associated microbiota and their subsequent effects on coral health are still not completely understood. In this study, we investigated the dynamics of three main microbial communities of the coral holobiont (e.g., Symbiodinium, bacteria and viruses), during an experimental thermal stress (+4 degrees C) conducted on the scleractinian Fungia repanda. The heat-treatment induced coral bleaching after 11 days and resulted in a final elevation of ca. 9, 130 and 250-fold in the abundance of mucosal viruses, bacteria, and Symbiodinium, respectively. On the contrary, the proportion of...
Tipo: Text
Palavras-chave: Coral bleaching; Thermal stress; Bacteria; Viruses; Holobiont; Vietnam.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00626/73823/73642.pdf

Bacterial colonization of winter flounder Pseudopleuronectes americanus fed live feed enriched with three different commercial diets
Provedor de dados: 5
Autores: Seychelles, L. H.; Audet, C.; Tremblay, R.; Lemarchand, K.; Pernet, Fabrice.
The proliferation of bacteria in intensive aquaculture systems may be responsible for poor growth and mass mortality of marine fish larvae. Essential fatty acids provided in the diet could protect larvae by modulation of the immune response via arachidonic acid (AA) and eicosapentaenoic acid (EPA). Winter flounder Pseudopleuronectes americanus larvae were fed rotifers Brachionus plicatilis enriched with three commercial diets containing different fatty acid profiles. Bacterial colonization on the gills and skin and in the intestinal lumen was evaluated at the end of the rotifer feeding period (day 26), and growth was surveyed until metamorphosis. At 26 days post hatching, larvae fed rotifers containing the higher AA content and with a higher...
Tipo: Text
Palavras-chave: Bacteria; Colonization; Fatty acids; Live feed; Winter flounder.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00031/14273/12399.pdf

Etude de la structure des communautés bactériennes du sédiment et de l'écologie de Vibrio aestuarianus pathogène de l'huître creuse Crassostrea gigas dans deux sites ostréicoles
Provedor de dados: 5
Autores: Azandegbe, Afi.
Since several years, the French oyster farming copes with high mortality affecting juveniles and some adults of the Pacific oyster Crassostrea gigas. Studies conducted in the framework of a multidisciplinary project (MOREST) showed that mortality was related to several factors like physiological and genetic status of oyster, pathogens including bacteria of the genus Vibrio, and revealed that the sediment, on which the oyster cages are installed, might play a particular role. This has been suggested because of the concomitance of the mortalities and increased toxic compounds like ammonia and sulphides in the sediment. Moreover, the presence of Vibrio bacteria in the sediment indicates a dual role of sediment in the mortality onset. The physico-chemical...
Tipo: Text
Palavras-chave: Huître; Crassostrea gigas; Bactéries; Vibrio aestuarianus; Écologie; Sédiment; Ammonium; Sulfures; Oyster; Crassostrea gigas; Bacteria; Vibrio aestuarianus; Ecology; Sediment; Ammonia; Sulphide.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00032/14277/11561.pdf

Experimental systems for the study of bacterial degradation of pollutants from the oil industry.
Provedor de dados: 5
Autores: Wardell, J; Brown, C.
The release of pollutants into the environment, either during normal operations or due to accidental discharges, has focussed attention on the fate and effects of xenobiotic compounds in the marine environment. This interest has been heightened by the development of the off-shore industry with the subsequent discharge of biocides from platforms. This paper describes the results obtained from investigations into the fate of biocides in the marine environment using continuous-culture techniques.
Tipo: Text
Palavras-chave: Bacteria; Continuous culture; Microbiological culture; Oil and gas industry; Pollutants; Pesticides; Biodegradation.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1018.pdf

Survival of bacteria and phytoplankton in ship's ballasts
Provedor de dados: 5
Autores: Masson, Daniel; Piquet, Jean-come; Courtois, Olivier.
Billions of tons of ballast water are lreased world-wide every year. Living marine, brackish or freshwater organisms, including those noxioux or unwanted, can travel by that way from a part of the world to another. A successful introduction needs : 1) survival during the pumping process 2) survival in ships' ballasts 3) survival and repreoduction in the new environment. To assess the survival conditions in ship's ballasts, a small scale pilot system has been built (MARTOB project), more realistic than laboratory vials and easier to handle than a real ballast. Bacteria and phytoflagellates coming from ship's sampling or from cultures were introduced in the system ; their survival in several waters or sediments are studied, a useful method to assess the...
Tipo: Text
Palavras-chave: Ballast water; Bacteria; Phytoplankton.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00074/18535/16079.pdf

Caloranaerobacter azorensis gen. nov., sp nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent
Provedor de dados: 5
Autores: Wery, Nathalie; Moricet, Jean-marc; Cueff, Valerie; Jean, Joelle; Pignet, Patricia; Lesongeur, Francoise; Cambon-bonavita, Marie-anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic bacterium, designated MV1087(T), was isolated from a deep-sea hydrothermal chimney sample collected from the Mid-Atlantic Ridge. The cells were straight, motile and stained Gram-negative. Growth was observed from 45 to 65 degreesC, with an optimum around 65 degreesC. No growth was observed at 40 or 70 degreesC. Growth was observed from pH 5.5 to 9.0 and the optimum pH was around 7. The salinity range for growth was 10-100 g sea salt l(-1) (corresponding to 6.5-65 g NaCl l(-1)) with an optimum at 30 g sea salt l(-1) (20 g NaCl l(-1)). Strain MV1087(T) was heterotrophic, able to ferment proteinaceous substrates, such as brain/heart infusion and gluten, and carbohydrates, such as glucose, xylan and starch. The...
Tipo: Text
Palavras-chave: Thermophile; Deep sea vent; Caloranaerobacter azorensis; Caloranaerobacter; Bacteria.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-586.pdf

Impact of the oyster Crassostrea gigas on a microbial community in Atlantic coastal ponds near La Rochelle
Provedor de dados: 5
Autores: Dupuy, Christine; Pastoureaud, Annie; Ryckaert, Mireille; Sauriau, Pierre-guy; Montanie, Hélène.
To assess the in situ impact of oysters Crassostrea gigas on planktonic protist and bacteria communities and the potential contribution of protozoa to their food resource intake, the abundance and the diversity of protists and bacteria were followed in 2 Atlantic coastal ponds, with and without oysters. The protist biomass in such ponds was high, with a maximum in spring of 982 mug C l(-1) and a minimum in winter of 179 pg C l(-1). Whatever the season, the presence of oysters (20 m(-2) corresponding to an average of 23 mg dry weight m(-2)) induced a significant decrease in &gt;5 pm protist abundance. On the contrary, planktonic organisms &lt;5 pm, such as Chlorophyta flagellates and bacteria, developed similarly in both ponds. It can be assumed that such...
Tipo: Text
Palavras-chave: Trophic link; Bacteria; Protists; Microbial food web; Coastal pond; Food source; Oysters; Bivalve.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-822.pdf

Transfert et mineralisation de la matiere organique azotee a travers les deux premiers echelons de la micro communaute heterotrophe
Provedor de dados: 5
Autores: Vanwambeke, F; Bianchi, M.
Bacterial response to organic (amino acids) or inorganic (NH sub(4) super(+)) nitrogen was studied in 350 liter closed batch systems. The experiments demonstrate an important NH sub(4) super(+) remineralization associated with bacterial growth. The differences between substrate and bacterial C/N ratios and bacterial metabolic demands partly explain the high values of ammonia produced. Grazing by heterotrophic microflagellates resulted in an important release of NH sub(4) super(+). This suggests and important role of grazers for the nitrogen remineralization in natural waters.
Tipo: Text
Palavras-chave: Flagellata; Ciliata; Bacteria; Plankton; Heterotrophic organisms; Ammonia; Amino acids; Nitrogen cycle; Mineralization.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-986.pdf

Interactions between a marine dinoflagellate (Alexandrium catenella) and a bacterial community utilizing reverine humic substances
Provedor de dados: 5
Autores: Carlsoon, Per; Edling, Hélène; Bechemin, Christian.
Dissolved organic matter in the form of riverine humic substances stimulated the growth of both axenic nitrogen-limited Alexandrium catenella cultures and nitrogen-limited cultures with a marine bacterial community present. The biomass increase of A. catenella could not be accounted for by utilization of inorganic nitrogen compounds. However, there was a considerable release of dissolved free and combined amino acids from the humic substances that was utilized by A. catenella. About 40% of the nitrogen used by A. catenella in the axenic treatment with humic substances added was taken up as organic nitrogen. Bacterial aminopeptidase and beta -glucosidase activity was stimulated by the addition of humic substances and bacterial growth increased several-fold....
Tipo: Text
Palavras-chave: Alexandrium catenella; Dinoflagellates; Bacteria; DOM; Humic substances.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/00173/28431/26763.pdf

Effect of P depletion on the functional pools of diatom carbohydrates, and their utilization by bacterial communities
Provedor de dados: 5
Autores: Labry, Claire; Delmas, Daniel; Moriceau, Brivaela; Gallinari, Morgane; Quere, Julien; Youenou, Agnes.
Phosphorus (P) limitation of phytoplankton growth is known to affect the accumulation and release of carbohydrates (CHO) by micro-algae. However, relatively little is known about the fate of algal exudates, notably their bacterial degradation. The CHO chemical characterization is also not exhaustive, especially in ‘functional’ pools relevant for phytoplankton physiology (particulate reserve [R] or structural [S] CHO) and for bacterial degradation (dissolved mono- [MDCHO] and polysaccharides [P-DCHO]). In this study, we investigated how P depletion and repletion affect the CHO composition in diatom Thalassiosira weissflogii cultures, and the shortterm response of free and diatom-attached bacteria in terms of abundance and potential βglucosidase activity...
Tipo: Text
Palavras-chave: P limitation; Phytoplankton; Diatom; Bacteria; Mineralization; Carbohydrate; Glucosidase.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00628/74045/73532.pdf

Etude experimentale de la colonisation de surfaces par les bacteries, en eau de mer circulante
Provedor de dados: 5
Autores: Fera, P; Prieur, D.
Before the building of an (OTEC) power plant, and experimental station was built near the entrance of the Bay of Brest in order to do preliminary studies. Using these facilities, the bacterial colonization of three substrata (stainless steel UB6, aluminium AG4, polycarbonate filters) was studied in an open seawater system, with a speed of 0,7 m s super(-1). The study was performed between May 1983 and May 1984, including seven experiments of 15 days each. Bacteria colonizing the metallic surfaces were counted by scanning electron microscopy, and bacteria colonizing the polycarbonate filters counted by epifluorescence microscopy. On the three substrata, bacterial colonization occurred in three successive stages: initial colonization, sometimes unsteady...
Tipo: Text
Palavras-chave: Fouling; OTEC plants; Bacteria; Colonization; Adhesion; Microbiological analysis; Surface films.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-964.pdf

Role of bacteria in the gastral cavity of Anthozoa.
Provedor de dados: 5
Autores: Herndl, G; Velimirov, B.
Bacterial density of the coelenteric fluid (CF) of some anthozoan species was determined in situ and under laboratory conditions. In all species examined bacterial density of the CF was higher in the gastral cavity than in ambient ranging from 10 super(5) up to 5 x 10 super(6) cells. ml super(-1). Incubation experiments with the CF indicate that a bacterial population within the coelenteron is efficiently controlled by the CF, favoring bacterial growth if bacterial densities are low in the CF and showing clearance activity at high bacterial densities. Feeding of pre-starved anthozoans caused a rapid response of coelenteric rod-shaped bacteria. Evidence is presented that coelenteric bacteria are digested periodically although they contribute 1% to the...
Tipo: Text
Palavras-chave: Anthozoa; Coelomic fluids; Bacteria; Microorganisms; Interspecific relationships; Metabolism; Nutrition.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-989.pdf

Evolution of bacterial communities precipitating carbonate, in Mediterranean lagoons water and sediment: Study on experimental conditions.
Provedor de dados: 5
Autores: Castanier, S; Maurin, A; Bianchi, A.
To determine if carbonate precipitation is an active or passive bacterial phenomena, factorial analysis has been realized with 880 strains isolated from different samples of sea water and muds kept in various conditions of incubation. Results show the existence of a strong anti-correlation between carbonate precipitation and amino-acid ammonification. Carbonate precipitation is not a passive phenomena linked with amino-acid ammonification.
Tipo: Text
Palavras-chave: MED; Biogeochemistry; Mathematical analysis; Water analysis; Sediments; Amino acids; Bacteria; Coastal lagoons; Chemical precipitation; Calcium carbonates.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-956.pdf

The dispersal of nitrogen-fixing Enterobacteriaceae from sewage into the waters and sediments of Morecambe Bay, UK.
Provedor de dados: 5
Autores: Jones, K; Betaieb, M.
The sediments of Morecambe Bay contain large numbers of salinity-tolerant, nitrogen-fixing Enterobacteriaceae (NFEs) mainly Klebsiella pneumoniae ) which appear to be derived from sewage. Sewage effluents, regardless as to whether they have received primary or secondary treatment, contain huge numbers of NFEs with salinity-tolerant and freshwater strains present in roughly equal quantities. The bacteria are discharged into the rivers and estuaries and carried out to sea. Once in the sea the salinity-tolerant strains retain their viability better than the freshwater strains so that the incoming tide, which doses the sediments, contains mainly salinity-tolerant NFEs. It is probable that similar processes occur all round the U.K. coast, as inshore coastal...
Tipo: Text
Palavras-chave: Klebsiella pneumoniae; Enterobacteriaceae; Salinity tolerance; Microbial contamination; Pathogenic bacteria; Bacteria; Sewage; Nitrogen fixation; Sediment pollution; Sea water.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1003.pdf

Etude experimentale de la disparition de coliformes fecaux d'un effluent urb ain en milieu marin
Provedor de dados: 5
Autores: Bonnefont, J; Lelong, P; Martin, Y.
The authors studied survival of faecal coliform (decrease rate + T90) in experimental devices (closed or continuous flow systems) using various dilutions of urban sewage in sea-water. Survival appears to be high (T90 between 12 to 81 h). Dilution level of sewage and organic matter contents seems to be important to explain the decrease of faecal coliforms observed in these experiments.
Tipo: Text
Palavras-chave: Bacteria; Microbial contamination; Pathogenic bacteria; Survival; Waste water; Marine pollution.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1009.pdf

Diversite de populations microbiennes thermophiles d'une cheminée hydrothermale océanique profonde : cultures d'enrichissement en bioréacteur et isolement d'espèces nouvelles
Provedor de dados: 5
Autores: Postec, Anne.
To explore the microbial diversity at deep sea vents, molecular techniques based on the analysis of the gene coding the 16S ribosomal RNA permitted to highlight a large archaeal and bacterial diversity. Nevertheless, the diversity of the hydrothermal microorganisms cultivated to date remains much lower than the diversity described with molecular tools. Innovative methods should therefore be used in order to cultivate new microorganisms, and also determine their metabolic properties and their potential role in the ecosystem. That was the purpose of this study: a bioreactor gas-lift was used to perform enrichment cultures in continuous, during 50 days, in thermophilic (60°C) and hyperthermophilic (90°C) conditions. A hydrothermal chimney sample (Rainbow...
Tipo: Text
Palavras-chave: Strain isolation and characterization; Hybridization; Sequencing; Cloning; DGGE; Gas lift bioreactor; Enrichment cultures; Deep sea hydrothermal vents; Bacteria; Archaea; Diversity; Isolement et caractérisation de souche; Hybridation; Séquençage; Clonage; DGGE; Bioréacteur gas lift; Cultures d'enrichissement; Sources hydrothermales océaniques profondes; Bacteria; Archaea; Diversité.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-367.pdf

Microbial activity at the deep water sediment boundary layer in two highly productive systems in the Western Mediterranean: the Almeria-Oran front and the Malaga upwelling
Provedor de dados: 5
Autores: Bianchi, A; Calafat, A; De Wit, R; Garcin, J; Tholosan, O; Cacho, I; Canals, M; Fabres, J; Grout, H; Masque, P; Sanchez-cabeza, Ja; Sempere, R.
Microbiological and biogeochemical studies were carried out in sediments and near bottom waters in the upwelling area off the Malaga coast and in the Almeria-Oran frontal zone. In these nutrient-rich conditions, metabolic activity is potentially limited by oxygen availability through the sediment depth. In the surficial sediments of the frontal zone, however, oxygen penetrated to a depth of 4 cm, allowing oxic mineralisation processes to occur throughout the layer. In the surficial sediments of the upwelling area, oxygen penetration was limited to the top 2.5 cm, leading to anoxic conditions. Glutamate respiration and global oxygen uptake rates were clearly higher than in the frontal zone. In the superficial sediments of the frontal zone, bacteria were...
Tipo: Text
Palavras-chave: Taux de minéralisation; Sédiments; Eaux profondes; Méditerranée occidentale; Bacteria; Mineralisation rate; Sediments; Deep-sea; Western Mediterranean.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00322/43298/43034.pdf

Etude d'oligosaccharides bioactifs issus d'exopolysaccharides bactériens : obtention, caractérisation et relation structure/fonction
Provedor de dados: 5
Autores: Roger, Olivier.
Unusual exopolysaccharides (EPS) produced by heterotrophic aerobic and mesophilic bacteria originating from hydrothermal vent, were reviewed as a new source of polysaccharidic structures endowed with innovative biological properties. The first step of this study was the structure determination of the EPS GY785, synthesized by Alteromonas infernus. A highly branched nonasaccharidic repetitive unit was identified using chemical modifications and nuclear magnetic resonance spectroscopy. This 106 g/mol polysaccharide is composed of glucose, galactose, glucuronic acid, galacturonic acid, and contains a single branched ramification and one sulphate group.  The second step was the preparation of low molecular weight bioactive polysaccharides presenting interest...
Tipo: Text
Palavras-chave: Phosphorylation; Sulphation; Depolymerization; NMR spectroscopy; Structure; Deep sea hydrothermal vent; Bacteria; Exopolysaccharides; Activité anticoagulante; Phosphatation; Sulfatation; Dépolymérisation; Spectroscopie de RMN; Structure; Sources hydrothermales; Bactéries; Exopolysaccharides.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/these-622.pdf

Étude des secteurs du Croisic et de Pen-Bé : estimation des apports continentaux et évaluation des stocks conchylicoles
Provedor de dados: 5
Autores: Gendronneau, Maud; Haure, Joel; Jeanneret, Hélène; Le Merrer, Yoann; Lequette, Camille; Lomakine, Cédric; Martin, Jean-louis; Papin, Mathias; Ponthoreau, Catherine; Robin, Marc.
Ifremer, Nantes University and the township community "CAP Atlantique" have joined their skills to try to better understand how two key conchological areas in the shore economy of the Pays de la Loire area develop, especially since, environmentally, little is known about them, and because they face recurrent issues on the zootechnic level. This programme encompasses two main topics: assessment of environmental inputs (catchments areas input, contaminants evolution in the area, and impact on the conchological zones) and a study of the conchological culture (growth, mortality, analysis of the stocks). Concomitantly, operational tools (hydrodynamic and flux transfer models, databases, SIG) are developed to improve local management of the sites and information...
Tipo: Text
Palavras-chave: Phytosanitary; Bacteria; SIG; Conchological stocks; Modelization; Catchment areas; Continental inputs; Pen B�; Le Croisic; Phytosanitary; Bacteria; SIG; Conchological stocks; Modelization; Catchment areas; Continental inputs; Pen B�; Le Croisic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00380/49129/49564.pdf

Influence d'une pollution chimique par l'apatite sur les peuplements bacteriens du golfe d'Aqaba (Jordanie)
Provedor de dados: 5
Autores: Vacelet, E.
The influence of spillage of phosphates on the bacterial population and its activity was found to be not significant (on total number) or significant but low (on the number of growing cells which was higher close to the loading area). Phosphate discharge resulted also in a significant increase of dividing cells and of enzymatic activity but its effects could be hardly separated from sewage effects. Neither solubilizing strains nor bacterial solubilization could be detected.
Tipo: Text
Palavras-chave: Bacteria; Apatite; Pollution effects; Phosphates; Chemical pollution.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1010.pdf

Field enhanced bacterial sample stacking in isotachophoresis using wide-bore capillaries
Provedor de dados: 5
Autores: Oukacine, Farid; Quirino, Joselito P.; Destoumieux-garzon, Delphine; Cottet, Herve.
The isotachophoretic analysis of different bacterial strains was studied using capillaries with different internal diameters from 50 to 250 mu m. Several injection modes were investigated and compared in order to improve the limit of detection of bacteria by capillary isotachophoresis. A system suitability test obtained from the separation voltage was developed to ensure reliable results. As expected, the use of wider bore capillaries improved the analytical sensitivity of the isotachophoretic method when compared to the 50 mu m capillary. With the optimized conditions, the isotachophoretic method presented in this work allows the quantification of Erwinia carotovora (Gram negative bacteria) with a limit of detection as low as similar to 3000 cells mL(-1)....
Tipo: Text
Palavras-chave: Capillary zone electrophoresis; Isotachophoresis; Bacteria; Electrokinetic injection; Field-enhanced sample injection.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00115/22636/20596.pdf

Microplastic bacterial communities in the Bay of Brest: Influence of polymer type and size
Provedor de dados: 5
Autores: Frere, Laura; Maignien, Lois; Chalopin, Morgane; Huvet, Arnaud; Rinnert, Emmanuel; Morrison, Hilary; Kerninon, Sandrine; Cassone, Anne-laure; Lambert, Christophe; Reveillaud, Julie; Paul-pont, Ika.
Microplastics (&lt;5 mm) exhibit intrinsic features such as density, hydrophobic surface, or high surface/volume ratio, that are known to promote microbial colonization and biofilm formation in marine ecosystems. Yet, a relatively low number of studies have investigated the nature of microplastic associated bacterial communities in coastal ecosystems and the potential factors influencing their composition and structure. Here, we characterized microplastics collected in the Bay of Brest by manual sorting followed by Raman spectroscopy and studied their associated bacterial assemblages using 16S amplicon high-throughput sequencing. Our methodology allowed discriminating polymer type (polyethylene, polypropylene and polystyrene) within small size ranges...
Tipo: Text
Palavras-chave: Bacteria; Microplastics; Coastal ecosystem; Metabarcoding; Vibrios.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00449/56082/57614.pdf

Biomass distribution and physiological capabilities of bacteria in the water column above a sea grass system.
Provedor de dados: 5
Autores: Velimirov, B; Herndl, G; Kavka, G.
The distribution pattern of bacteria in the water column above and within a Posidonia oceanica bed near Ischia (off the Gulf of Naples) was investigated and correlated with DOC and POM data for autumn and winter. Data from samples of 5 stations along a transect from shallow to deep showed rather low bacterial densities (10 super(4).ml super(-1)) for both seasons. The number of attached bacteria was higher in late winter as compared to autumn when the leaves are shed from the stands. Except for occasional peaks DOC ranged between 1.5 and 4.5 mg C/1 while POM concentrations ranged form 4 to 14 mg AFDW.1 super(-1). Hydrolytic capabilities of isolated bacterial colonies showed that more carbohydrate substrates could be fermented in winter as compared to...
Tipo: Text
Palavras-chave: Posidonia oceanica; Microorganisms; Physiology; Seasonal distribution; Algae; Bacteria; Particulate organic matter; Metabolism; Water column; Fermentation; Sea grass.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-954.pdf

Recent advances in Australian prawns diseases and pathology
Provedor de dados: 5
Autores: Owens, L; Hall, M.
Disease is always limiting in animal production and new syndromes occur in fledgling industries. This paper describes the latest findings in this area.
Tipo: Text
Palavras-chave: Vibrio damsela; Macrobrachium rosenbergii; Malacostraca; Bacteria; Septicaemia; Aetiology; Viruses; Juveniles; Ovaries; Larvae; Pathology; Viral diseases.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/1989/acte-1461.pdf

Traitement de l''eau par ultraviolets, application a la purification des coquillages
Provedor de dados: 5
Autores: Cailleres, J.
The germicide properties of ultra-violet radiation in a wave length ranging from 100 to 400 mm were already discovered back in the late 19th century. These properties specific to UV-C radiation (200-280 mm) result from its action on the nucleic acids of the micro-organisms. Each micro-organism, depending on its composition, therefore presents a specific sensitivity to this radiation.
Tipo: Text
Palavras-chave: France; Escherichia coli; Crassostrea; Bivalvia; Bacteria; Ultraviolet radiation; Self purification; Shellfish culture.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1629.pdf

Photoproduction d'hydrogene par une souche de bacteries phototrophes sulfureuses marines
Provedor de dados: 5
Autores: Matheron, R; Baulaigue, R.
H sub(2) evolution from sulfur and organic compounds by Ectothiorhodospira) strain BA1011 has been investigated. Ectothiorhodospira strain BA1011 produces hydrogen from sulfide, thiosulfate and some organic acids such as propionate, acetate, lactate and succinate. Major characteristics are defined from sulfide: experiments show a correlation between photohydrogen production and CO sub(2) assimilation as well as an increase of the utilization of endogeneous and exogeneous organic substrates with sulfide as electron donor.
Tipo: Text
Palavras-chave: Ectothiorhodospira; Bacteria; Hydrogen; Photochemistry; Photochemical reactions.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-969.pdf

In situ structuring of virioplankton through bacterial exoenzymatic activity: interaction with phytoplankton
Provedor de dados: 5
Autores: Ory, Pascaline; Palesse, S.; Delmas, Daniel; Montanie, Helene.
The abundance and composition of microbial communities were investigated during 2007 in Marennes Oleron Bay (France) in order to characterize the biological relationships governing the planktonic food web. Compared to the results of previous years, there was a lower abundance and a lower magnitude of variations in autotrophic and heterotrophic microbial entities in 2007. Using a Spearman rank correlation analysis, a significant link for biological abundance was only noted between bacteria and ciliates. However, an interesting relationship was highlighted between chlorophyll a and bacterial aminopeptidase and beta-glucosidase exoenzymatic activities. Two characteristic periods of virus-bacteria uncoupling were observed in spring and autumn 2007. Using...
Tipo: Text
Palavras-chave: Virus; Bacteria; Phytoplankton; Diversity; Aminopeptidase.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00050/16099/13571.pdf

Microbial community structure in the sea surface microlayer at two contrasting coastal sites in the northwestern Mediterranean Sea
Provedor de dados: 5
Autores: Joux, Fabien; Agogue, Helene; Obernosterer, Ingrid; Dupuy, Christine; Reinthaler, Thomas; Herndl, Gerhard J.; Lebaron, Philippe.
In an attempt to compare the microbial community structure between the sea surface microlayer (SML) and subsurface waters (SSW), we determined the enrichment factors (EF: the ratio of abundance or activity in the SML to abundance or activity in SSW) of 13 biological parameters. Samples were taken at 2 contrasting coastal sites in the Mediterranean Sea, corresponding to a high (Barcelona, Spain) and low (Banyuls-sur-Mer, France) urbanized area. Principal component analysis showed that temporal variability was much higher at Barcelona than at Banyuls, and that the characteristics of the SML and SSW samples were more closely related at Barcelona. At both sites, the SML was weakly enriched in heterotrophic bacteria (on average 1.1-fold), Synechococcus spp. (on...
Tipo: Text
Palavras-chave: Sea surface microlayer; Enrichment factor; Microbial structure; Virus-like particles; Bacteria; Microalgae; Nanoflagellates.
Ano: 2006
URL: https://archimer.ifremer.fr/doc/00243/35434/33961.pdf

Clostridium caminithermale sp nov., a slightly halophilic and moderately thermophilic bacterium isolated from an Atlantic deep-sea hydrothermal chimney
Provedor de dados: 5
Autores: Brisbarre, Nadège; Fardeau, Marie-laure; Cueff, Valerie; Cayol, Jean-luc; Barbier, Georges; Cilia, Valérie; Ravot, Gilles; Thomas, Pierre; Garcia, Jean-louis; Ollivier, Bernard.
A strictly anaerobic, slightly halophilic and moderately thermophilic, sporulating rod designated strain DVird3(T) was isolated from deep-sea hydrothermal vent samples collected at a depth of approximately 800 m on the Atlantic Ocean Ridge. Strain DVird3(T) possessed a few laterally inserted flagella, had a DNA G+C content of 33.1 mol% and grew optimally at pH 6.6 and at 45 degreesC. Growth was observed at temperatures between 20 and 58 degreesC and at pH values between 5.8 and 8.2. The optimum NaCl concentration for growth was 3% sea salt (30 g l(-1)); no growth was observed in the presence of 15 or 60 g sea salt l(-1). Strain DVird3(T) is heterotrophic and utilizes some sugars and various single amino acids. Acetate was the main fatty acid detected from...
Tipo: Text
Palavras-chave: Phylogeny; Thermophile; Bacteria; Deep sea hydrothermal vent.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-588.pdf

Cultivation-Assisted Genome of Candidatus Fukatsuia symbiotica; the Enigmatic "X-Type" Symbiont of Aphids
Provedor de dados: 5
Autores: Patel, Vilas; Chevignon, Germain; Manzano-marin, Alejandro; Brandt, Jayce W.; Strand, Michael R.; Russell, Jacob A.; Oliver, Kerry M.
Heritable symbionts are common in terrestrial arthropods and often provide beneficial services to hosts. Unlike obligate, nutritional symbionts that largely persist under strict host control within specialized host cells, heritable facultative symbionts exhibit large variation in within-host lifestyles and services rendered with many retaining the capacity to transition among roles. One enigmatic symbiont, Candidatus Fukatsuia symbiotica, frequently infects aphids with reported roles ranging from pathogen, defensive symbiont, mutualism exploiter, and nutritional co-obligate symbiont. Here, we used an in vitro culture-assisted protocol to sequence the genome of a facultative strain of Fukatsuia from pea aphids (Acyrthosiphon pisum). Phylogenetic and genomic...
Tipo: Text
Palavras-chave: Heritable symbiont; Bacteria; Comparative genomics; Insect; Evolutionary transition.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00635/74663/74594.pdf

Characterisation of volatile compounds produced by bacteria isolated from the spoilage flora of cold-smoked salmon
Provedor de dados: 5
Autores: Joffraud, Jean-jacques; Leroi, Francoise; Roy, Caroline; Berdague, J.
This study investigated the volatile compounds produced by bacteria belonging to nine different bacterial groups: Lactobacillus sake, L. farciminis, L. alimentarius. Carnobacterium piscicola, Aeromonas sp., Shewanella putefaciens. Brochothrix thermosphacta, Photobacterium phosphoreum and Enterobacteriaceae isolated from cold-smoked salmon. Each bacterial group was represented by several strains. In addition, combinations of the groups were examined as well. Sterile blocks of cold-smoked salmon were inoculated, vacuum-packed and stored at 6 degreesC. After 40 days of storage at 6 degreesC, aerobic viable count and pH were recorded, the volatile fraction of the samples was analysed by gas chromatography-mass spectrometry (GC-MS). and spoilage was assessed by...
Tipo: Text
Palavras-chave: Volatile compounds; Bacteria; Spoilage; Cold smoked salmon.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-492.pdf

Polychaete tube walls as zonated microhabitats for marine bacteria.
Provedor de dados: 5
Autores: Reichardt, W.
The predominantly anoxic, sulfide-rich sandy sediment of a shallow lagoon at Kiel Fjord was densely inhabited by the polychaete worm Nereis diversicolor (280 cm super(3) m super(-2) of biomass volume). Burrow walls which contrasted by their brown coloration of at least 1.5 mm thickness with the bulk of the reduced black sediment, made up for 6% of the total volume of the 10 cm cores investigated. Only the uppermost mm of the internal surface layer contained detectable O sub(2). As a result of the build up of internal redox gradients, CO sub(2) dark fixation was activated in the burrow walls (by a factor of approximately equals 2). Assays of ribulose-biphosphate carboxylase activities and viable counts of potentially chemoautotrophic bacteria in different...
Tipo: Text
Palavras-chave: Nereis diversicolor; Polychaeta; Bacteria; Microorganisms; Carbon cycle; Tube dwellers; Carbon fixation; Burrows; Bioturbation; Sediments.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-990.pdf

Siderophore production and probiotic effect of Vibrio sp. associated with turbot larvae, Scophthalmus maximus
Provedor de dados: 5
Autores: Gatesoupe, Fj.
The proportion of vibrio E, dominant in healthy turbot larvae, was artificially increased in the rotifer enrichment medium. The maid effect of this vibrio enrichment was to improve the resistance of larval turbot challenged with a pathogenic strain of Vibrio splendidus, vibrio P. The improvement of the survival rates was significant with 1.5 x 10(6) CFU of vibrio E added per mi of enrichment medium, and it was still maximum at the hi hest dose tested (5 x 10(7) CFU.ml(-1); 80 +/- 2% of survivors after 48 h of challenge, versus 50 +/- 2 % for the control group). Further in vitro characterisation showed that vibrio E was able to grow in the presence of the chelating agents EDDHA and EDTA, while producing siderophore, as revealed by chrome azurol S dyeing....
Tipo: Text
Palavras-chave: Bacteria; Pathogenic Vibrio; Probiotic Vibrio; Experimental infection; Siderophore; Turbot; Larva.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00065/17671/15199.pdf

Reduction du nitrate dans les sediments marins. Comparaison des activites mesurees in vitro et in situ
Provedor de dados: 5
Autores: Esteves, J; Bonin, P; Blanc, F; Mille, G; Bertrand, J.
In vitro nitrate reduction in hydrocarbon-polluted marine sediments has been studied by flow-through system. By analysing the rate of nitrate comsumption as a function of nitrate concentration the authors determined the Michaelis-Menten kinetics parameters. (km = 330 mu M; vax = 2.0 mu M/g super(-1)/h super(-1). The authors found a value of 60 mu M/m super(-2)/h super(-1) for the rate of in vitro nitrate consumption derived from the above kinetic parameters and from the nitrate concentration in the sediment. In parallel a value of 67.4 mu M/m super(-2)/h super(-1) was found for the rate of nitrate consumption under in situ conditions. The great similarity between both results would favor the in vitro technique as a valid and more convenient one.
Tipo: Text
Palavras-chave: MED; Biogeochemistry; Oil pollution; Methodology; Environmental conditions; Experimental research; Comparative studies; Metabolism; Bacteria; Sediment chemistry; Reduction; Nitrates; Denitrification; Nitrogen cycle.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-979.pdf

Spoilage potential and sensory profile associated with bacteria isolated from cold-smoked salmon
Provedor de dados: 5
Autores: Stohr, Valerie; Joffraud, Jean-jacques; Cardinal, Mireille; Leroi, Francoise.
Off-odours/flavours associated with cold-smoked salmon spoilage are due to the activity of microflora. This study evaluated the spoilage potential of nine bacterial groups (Shewanella putrefaciens, Brochothrix thermosphacta, Aeromonas spp., Lactobacillus alimentarius, Lactobacillus sake,Lactobacillus farciminis, Carnobacterium piscicola, Photobacterium phosphoreum and Serratia liquefaciens) isolated from cold-smoked salmon. Five different isolates from each group were inoculated into sterile cold-smoked salmon blocks, and chemical and sensory changes were studied after five weeks of storage in vacuum packs at 6 degreesC. Bacterial growth was monitored weekly during the storage period. A sensory profile was assigned to each group, Principal component...
Tipo: Text
Palavras-chave: Sensory analysis; Spoilage potential; Bacteria; Cold smoked salmon.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-578.pdf

Effect of ionizing radiation onVibrio bacteria in Crassostrea virginica (American oyster)
Provedor de dados: 5
Autores: Eberiel, D; Dooley, K; Langton, L; Malleth, J.
The presence of Vibrio bacteria in raw shellfish poses a significant health hazard to the consuming public. These human pathogens are natural, seasonal components of the microbial flora of shellfish which can result in primary septicemic or diarrheal fatal infections. Existing shellfish depuration neither targets nor eliminates the Vibrio bacteria. For this reason, additional purification processes are necessary. In this study, the effect of low dose gamma radiation on the inactivation ofVibrio cholerae (01 and non 01) and vulnificus is investigated. Log decrement (D10) values ranging only to a high of 0.09 KGy in the Vibrio strains support the hypothesis that food irradiation technology can provide an additional effective and efficient shellfish...
Tipo: Text
Palavras-chave: USA Coasts; USA; Vibrio vulnificus; Vibrio cholerae; Crassostrea virginica; Bivalvia; Vibrio; Bacteria; Self purification; Marine molluscs; Irradiation; Gamma radiation; Ionizing radiation; Oyster culture.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1619.pdf

Bacterivory of a mudflat nematode community under different environmental conditions
Provedor de dados: 5
Autores: Pascal, P.y.; Dupuy, C; Richard, P; Rzeznik-orignac, J; Niquil, Nathalie.
The fate of the benthic bacterial biomass is a topic of major importance in understanding how softbottom environments function. Because of their high abundance, production and nutritional value, benthic bacteria may constitute an important food resource for benthic fauna. The trophic role of bacteria for a nematode community on the Brouage mudflat (Marennes-Ole´ron-France), dominated by three species: Chromadora macrolaima (64% of the abundance), Daptonema oxycerca (15%) and Ptycholaimellus jacobi (8%), was determined in grazing experiments using 15N pre-enriched bacteria. On intertidal flats, seasonal, tidal and circadian cycles induce strong variations in environmental conditions. Grazing experiments were performed in order to measure the effects of...
Tipo: Text
Palavras-chave: Mudflat; Environmental factor; Grazing; Bacteria; Nematode.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4295.pdf

Biodegradation du petrole en mer. Influence de l'apport d'azote et de phosphore sous forme minerale
Provedor de dados: 5
Autores: Lepetit, J; Guiot, J; Martin, Y; Tagger, S.
To exploit the presence in the sea of microorganisms capable of degrading petroleum products a mineral fertilizer, containing immediatly assimilable nitrogen and phosphorus compounds, was used to increase the microbial population and therefore, the degradation potential of treated polluted waters. The fertilizer supply caused an increase in heterotrophic and hydrocarbon-splitting bacteria and an increase in the phytoplanktonic biomass. The latter is characterized by an increase in a few selected species; it constitutes an important supply of organic substances, and it is responsible for increasing the dissolved oxygen which is essential for petroleum oxidation. Supplying the fertilizer results in an increase in the treated water degradation potential and a...
Tipo: Text
Palavras-chave: Bacteria; Microorganisms; Heterotrophic organisms; Phosphorus compounds; Nitrogen compounds; Fertilizers; Hydrocarbons; Biodegradation; Sea water.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1020.pdf

Variations metaboliques des communautes bacteriennes lors d'une pollution par petrole brut, avec ou sans traitement
Provedor de dados: 5
Autores: Cauchi, B.
Metabolic potentialities of bacterial communities in marine water after Crude Oil pollution (Arabian Light) and in marine water after pollution and treatment (enrichment: Inipol EAP 22) have been studied and compared. With data analysis: regressions, hierarchical ascending classification and multifactor correspondence analysis, the treatment gives rise to conservation and one increase of enzymatic pool (second day) of the bacterial sample as a combined metabolic homogeneity contrary to pollution impact. The direct assimilation of hydrocarbons looks more regular and slow without treatment.
Tipo: Text
Palavras-chave: Bacteria; Data processing; Pollution effects; Crude oil; Marine pollution; Physiology; Metabolism; Microbiology.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1023.pdf

Bacterial inactivation using pulsed light
Provedor de dados: 5
Autores: Elmnasser, Noura; Ritz, Magali; Leroi, Francoise; Orange, Nicole; Bakhrouf, Amina; Federighi, Michel.
Pulsed light is a new method intended for the decontamination of food surfaces using short, high frequency pulses of an intense broad spectrum. The effects of broad spectrum pulsed light on the survival of Listeria monocytogenes Scott A, Listeria monocytogenes CNL, Pseudomonas fluorescens MF37 and Photobacterium phosphoreum SF680 populations on agar and in a liquid medium were investigated during this study. The sterilisation system generated 1.5 J cm(-2) per pulse with eight lamps for 300 mu s. In the case of surface-seeded cells, a 7.8, 8.14 and &gt; 7.14 log reduction was obtained for L. monocytogenes, Ps. fluorescens MF37 and Ph. phosphoreum, respectively, after a single pulse of treatment. Inactivation levels were lower for depth-plated cells: indeed,...
Tipo: Text
Palavras-chave: Efficiency; Inactivation; Bacteria; Treatment; Pulsed light.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3041.pdf

Spatiotemporal variations in microbial diversity across the three domains of life in a tropical thalassohaline lake (Dziani Dzaha, Mayotte Island)
Provedor de dados: 5
Autores: Hugoni, Mylene; Escalas, Arthur; Bernard, Cecile; Nicolas, Sebastien; Jezequel, Didier; Vazzoler, Fanny; Sarazin, Gerard; Leboulanger, Christophe; Bouvy, Marc; Got, Patrice; Ader, Magali; Troussellier, Marc; Agogue, Helene.
Thalassohaline ecosystems are hypersaline environments originating from seawater in which sodium chloride is the most abundant salt and the pH is alkaline. Studies focusing on microbial diversity in thalassohaline lakes are still scarce compared with those on athalassohaline lakes such as soda lakes that have no marine origin. In this work, we investigated multiple facets of bacterial, archaeal and eukaryotic diversity in the thalassohaline Lake Dziani Dzaha using a metabarcoding approach. We showed that bacterial and archaeal diversity were mainly affected by contrasting physicochemical conditions retrieved at different depths. While photosynthetic microorganisms were dominant in surface layers, chemotrophic phyla (Firmicutes or Bacteroidetes) and...
Tipo: Text
Palavras-chave: Archaea; Bacteria; Eukaryotes; Extreme environment; Metabarcoding; Thalassohaline lake.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00464/57564/59738.pdf

Seasonal variation of microbial population from sediments of Vellar Estuary, South India.
Provedor de dados: 5
Autores: Lakshmanaperumalsamy, P; Chandramohan, D; Natarajan, R.
A seasonal study on the occurrence and distribution of total aerobic microbial population (TMP), total aerobic heterotrophic bacteria (THB), actinomycetes and fungi in the sediments of Vellar Estuary, South India (11 degree 29'N 79 degree 46'E) was carried out during Jan. to Dec. 1975. Sediment samples were collected at monthly intervals, from 4 stations and analysed microbiologically. In general, bacterial population was found to be dominant followed by actinomycetes and fungi. The max peak for TMP, THB and fungi were recorded in Nov. in all stations. Monsoon season supported TMP, THB and fungi to a high level and it was summer for actinomycetes. Statistical analysis (Kendall's tau) showed a significantly positive correlation among TMP, THB, actinomycetes...
Tipo: Text
Palavras-chave: Actinomycetes; Population number; Statistical analysis; Seasonal variations; Fungi; Bacteria; Sediment analysis; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-943.pdf

Microbial interactions in marine water amended by eroded benthic biofilm: A case study from an intertidal mudflat
Provedor de dados: 5
Autores: Montanie, Helene; Ory, Pascaline; Orvain, Francis; Delmas, Daniel; Dupuy, Christine; Hartmann, Hans J.
In shallow macrotidal ecosystems with large intertidal mudflats, the sediment-water coupling plays a crucial role in structuring the pelagic microbial food web functioning, since inorganic and organic matter and microbial components (viruses and microbes) of the microphytobenthic biofilm can be suspended toward the water column. Two experimental bioassays were conducted in March and July 2008 to investigate the importance of biofilm input for the pelagic Microbial and Viral Loops. Pelagic inocula (&lt; 0.6 μ- and &lt; 10 μ filtrates) were diluted either with &lt; 30 kDa-ultrafiltered seawater or with this ultrafiltrate enriched with the respective size-fractionated benthic biofilm or with &lt; 30 kDa-benthic compounds (BC). The kinetics of heterotrophic...
Tipo: Text
Palavras-chave: Mudflats; Virus; Bacteria; HNF; Coastal water.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00166/27691/25883.pdf

Co-occurring nematodes and bacteria in submarine canyon sediments
Provedor de dados: 5
Autores: Rzeznik-orignac, Jadwiga; Puisay, Antoine; Derelle, Evelyne; Peru, Erwan; Le Bris, Nadine; Galand, Pierre E.
In submarine canyon sediments, bacteria and nematodes dominate the benthic biomass and play a key role in nutrient cycling and energy transfer. The diversity of these communities remains, however, poorly studied. This work aims at describing the composition of bacteria and nematode communities in the Lacaze-Duthiers submarine canyon in the north-western Mediterranean Sea. We targeted three sediment depths for two consecutive years and investigated the communities using nuclear markers (18S rRNA and 16S rRNA genes). High throughput sequencing combined to maximal information coefficient (MIC) statistical analysis allowed us to identify, for the first time, at the same small scale, the community structures and the co-occurrence of nematodes and bacteria...
Tipo: Text
Palavras-chave: Bacteria; Meiofauna; Canyon Lacaze-Duthiers; Metabarcording; Molecular approach; Mediterranean Sea; Nematodes; Marine biodiversity; 18S; 16S.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00454/56586/76447.pdf

Transmission Electron Microscopy Study of the Neonatal Rat Tongue Mucosa Treated with Special Attention to the Bacteriae on the Epithelial Cell Membrane
Provedor de dados: 77
Autores: Picoli,Lara Cristina; Lopes,Ruberval A; Semprini,Marisa; Sala,Miguel Angel; Ogawa,Koichi; Watanabe,Ii-sei.
The epithelial cell surfaces of neonatal Wistar rat tongue mucosa were examined by transmission electron microscopic method. The tissues were fixed by modified Karnovksy solution and embedded in Spurr resin. Thin sections of 90 nm thickness were cut and examined in the Jeol 1010 transmission electron microscopy. It was revealed that the queratinized squamous epithelial cells of the tongue mucosa exposed to cadmium presented groupings of numerous bacteriae which are attached to the flat cell membrane surfaces. The coccus and bacillus are attached to the cell membrane in organized rows or at random, as clearly seen in the TEM images. At high magnification, the transmission electron microscopic images showed that bacteriae are attached by a fibrillar...
Tipo: Journal article
Palavras-chave: Epithelial cell; Tongue mucosa; Bacteria; Cadmium; Transmission electron microscopy.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300006

DNA Checkerboard Method for Bacterial Pathogen Identification in Oral Diseases
Provedor de dados: 77
Autores: Nascimento,Cássio do; Issa,João Paulo Mardegan; Watanabe,Evandro; Ito,Izabel Yoko.
This work aim to show by literature review the principal characteristics of the DNA checkerboard method for bacterial pathogens identification in oral diseases, showing the most varieties uses and applications of this technique
Tipo: Journal article
Palavras-chave: DNA Checkerboard; DNA probes; Microbiology; Bacteria.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500017

IN VITRO ACTIVITY OF PROPOLIS AGAINST BACTERIAL AND  YEAST PATHOGENS ISOLATED FROM HUMAN INFECTIONS
Provedor de dados: 79
Autores: FERNANDES Jr.,A.; SUGIZAKI,M.F.; FOGO,M.L.; FUNARI,S.R.C.; LOPES,C.A.M.
The in vitro activity of propolis against 118 Staphylococcus aureus, 108 Escherichia coli, 60 Salmonella typhimurium, 50 Candida albicans, 23 Candida parapsilosis, 19 Candida tropicalis and 14 Candida guilliermondii isolated from human infections was studied by the agar dilution method. Among the bacteria, the Gram-negative were the least susceptible organisms showing minimal inhibitory concentration (MIC) for 90% of the strains tested ranging from 22.5 mg/ml - 10,2% V/V to 23.1 mg/ml - 10.5% V/V. The MIC's for Candida ranged from 0.80 mg/ml to &amp;gt; 11 mg/ml (0.40% V/V to &amp;gt; 5.0% V/V), the strains of C. parapsilosis being the least susceptible. The relative order of susceptibility among all isolates, was: S.aureus &amp;gt; C. tropicalis &amp;gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Antimicrobial activity; Bacteria; Yeast pathogens; Candida sp.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000200003

Microbiological analysis of water used in hydrotherapy
Provedor de dados: 80
Autores: Perestrelo,M. F.; Norberg,A. N.; Guerra-Sanches,F.; Torres,A. C.; Pile,E.
Water used in hydrotherapy units of Nova Iguaçu and Nilópolis, Rio de Janeiro State, Brazil, was microbiologically analyzed. Thirty samples (5ml each) were weekly collected from September 2001 to June 2002 before the beginning and after the end of activities in the units. For analysis, routine techniques were used, which showed the presence of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp, Candida spp, Penicillium spp, Epidermophyton spp, Aspergillus fumigatus, Aspergillus niger, Aspergillus spp, Cephalosporium spp, Cladosporium spp, Trichophyton rubrum, and Trichophyton spp. Results indicated a need for improving hygienic conditions, suggesting that water might be a contamination source in the evaluated units.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrotherapy; Water; Microorganisms; Bacteria; Fungi.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300006

A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide
Provedor de dados: 80
Autores: Clement,Herlinda; Flores,Vianey; Diego-Garcia,Elia; Corrales-Garcia,Ligia; Villegas,Elba; Corzo,Gerardo.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecticidal peptides; Protein expression; Bacteria; Theraphosid spider; Chemical synthesis; Cysteine-rich peptides.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100324

Jaburetox: update on a urease-derived peptide
Provedor de dados: 80
Autores: Becker-Ritt,Arlete Beatriz; Portugal,Camila Saretta; Carlini,Célia Regina.
Abstract Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal and insecticidal properties. Urease is toxic to insects and fungi per se but part of this toxicity relies on an internal peptide (~11 kDa), which is released upon digestion of the protein by insect enzymes. A recombinant form of this peptide, called jaburetox (JBTX), was constructed using jburell gene as a template. The peptide exhibits liposome disruption properties, and insecticidal and fungicidal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptide; Bacteria; Membranes; Nanoparticles.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100211

Selection for resistance to the rhizoctonia-bacterial root rot complex in sugar beet
Provedor de dados: 18
Autores: Strausbaugh, C.A.; Eujayl, Imad A.; Foote, P.
The Rhizoctonia-bacterial root rot complex continues to be a concerning problem in sugar beet production areas. To investigate resistance to this complex in 26 commercial sugar beet cultivars, field studies and greenhouse studies with mature roots from the field were conducted with Rhizoctonia solani AG-2-2 IIIB strains and Leuconostoc mesenteroides. Based on means for the 26 cultivars in the 2010 and 2011 field studies, fungal rot ranged from 0 to 8%, bacterial rot ranged from 0 to 37%, total rot ranged from 0 to 44%, and surface rot ranged from 0 to 52%. All four rot variables resulted in significant (P &lt; 0.0001) cultivar differences. Based on regression analysis, strong positive relationships (r2 from 0.6628 to 0.9320; P &lt; 0.0001) were present...
Tipo: Article
Palavras-chave: Root rots; Sugarbeet; Bacteria.
Ano: 2013
URL: http://eprints.nwisrl.ars.usda.gov/1475/1/1440..pdf

Influence of Sugarbeet Tillage Systems on the Rhizoctonia-Bacterial Root Rot Complex
Provedor de dados: 18
Autores: Strausbaugh, C.A.; Eujayl, Imad A.
The Rhizoctonia-bacterial root rot complex in sugarbeet caused by Rhizoctonia solani and Leuconostoc mesenteroides can cause significant yield losses.  To investigate the impact of different tillage systems on this complex, field studies were conducted from 2009 to 2011.  Split blocks with conventional and strip tillage as main plot treatments were arranged in a randomized complete block design with four replications.  Within main plots, there were seven treatments (non-inoculated check and six R. solani AG-2-2 IIIB strains).  Regardless of tillage, the roots responded in a similar manner for fungal rot (conventional 8% versus strip 7%), bacterial rot (26% versus 34%), total rot (33% versus 41%), neighboring roots infected (1.7 roots versus 1.5 roots),...
Tipo: Article
Palavras-chave: Root rots; Sugarbeet; Bacteria.
Ano: 2012
URL: http://eprints.nwisrl.ars.usda.gov/1472/1/1437.pdf

Influence of Rhizoctonia-bacterial Root Rot Complex on Storability of Sugarbeet
Provedor de dados: 18
Autores: Strausbaugh, C.A.; Rearick, E.; Eujayl, Imad A.; Foote, P.
The Rhizoctonia-bacterial root rot complex can lead to yield loss in the field but may also lead to problems with sucrose loss in storage.  Thus, studies were conducted to investigate if placing sugarbeet roots suffering from root rot together with healthy roots could compromise the ability of the healthy roots to retain sucrose.  Over a three year period, root samples from three commercial cultivars were compared in storage as a healthy (eight healthy roots) or rotted (eight healthy roots + one rotted root) treatment inside an outdoor storage pile.  The experiment was arranged as a split block (healthy in one half of block and rotted in the other) with the whole blocks arranged in a randomized complete block design with four replications.  Samples were...
Tipo: Article
Palavras-chave: Root rots; Sugarbeet; Bacteria.
Ano: 2011
URL: http://eprints.nwisrl.ars.usda.gov/1484/1/1406.pdf

Rhizoctonia and Bacterial Root Rot in Sugarbeet
Provedor de dados: 18
Autores: Strausbaugh, C.A.; Eujayl, Imad A.
Tipo: Article
Palavras-chave: Bacteria; Sugarbeet.
Ano: 2010
URL: http://eprints.nwisrl.ars.usda.gov/1360/1/1337.pdf

Bacteria and yeast associated with sugar beet root rot at harvest in the Intermountain West
Provedor de dados: 18
Autores: Strausbaugh, C.A.; Gillen, A.M.
Tipo: Article
Palavras-chave: Bacteria.
Ano: 2008
URL: http://eprints.nwisrl.ars.usda.gov/1260/1/1237.pdf

Occurrence of Wolbachia in Brazilian samples of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)
Provedor de dados: 82
Autores: Rocha,Lincoln S.; Mascarenhas,Rodrigo O.; Perondini,André L.P.; Selivon,Denise.
Wolbachia bacteria were detected by PCR followed by sequencing of a fragment of the 16S ribosomal gene, in a natural population sample and in two laboratory colonies of the medfly, C. capitata (Wied.), from Brazil. Sequencing revealed that the fragment was compatible with the Wolbachia type A group found in several insect species. This is the first description of a natural Wolbachia infection in C. capitata, since several other samples from different regions so far analyzed seemed to be free of infection.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bacteria; Medfly; Endosymbiont.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000600020

Microbial control of arthropod pests of tropical tree fruits
Provedor de dados: 82
Autores: Dolinski,Claudia; Lacey,Lawrence A.
A multitude of insects and mites attack fruit crops throughout the tropics. The traditional method for controlling most of these pests is the application of chemical pesticides. Growing concern on the negative environmental effects has encouraged the development of alternatives. Inundatively and inoculatively applied microbial control agents (virus, bacteria, fungi, and entomopathogenic nematodes) have been developed as alternative control methods of a wide variety of arthropods including tropical fruit pests. The majority of the research and applications in tropical fruit agroecosystems has been conducted in citrus, banana, coconut, and mango. Successful microbial control initiatives of citrus pests and mites have been reported. Microbial control of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Virus; Fungus; Nematode.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2007000200001

Bacillus thuringiensis survey in Brazil: geographical distribution and insecticidal activity against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)
Provedor de dados: 82
Autores: Valicente,Fernando H.; Barreto,Marliton R.
A total of 3408 strains of Bacillus thuringiensis were collected from 1448 soil samples in 10 Brazilian states, four different geographical regions, covering 96 counties. These strains were evaluated against Spodoptera frugiperda (J.E. Smith) larvae. Only 62% killed between 81% and 100% and 1758 caused no mortality. Highest proportion of efficient strains (larval mortality above 75%) was found from the total isolated per region in the South Region (16.6%), followed by Western Central (3.1%), Southeast (1.1%) and Northeast Region (0.4%).
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Insect; Bacteria; Native isolate; Biological control; Pathology.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2003000400014

The Role of Carbohydrates in Infection Strategies of Enteric Pathogens
Provedor de dados: 19
Autores: Kato, Kentaro; Ishiwa, Akiko; 加藤, 健太郎.
Enteric pathogens cause considerable public health concerns worldwide including tropical regions. Here, we review the roles of carbohydrates in the infection strategies of various enteric pathogens including viruses, bacteria and protozoa, which infect the epithelial lining of the human and animal intestine. At host cell entry, enteric viruses, including norovirus, recognize mainly histo-blood group antigens. At the initial step of bacterial infections, carbohydrates also function as receptors for attachment. Here, we describe the function of carbohydrates in infection by Salmonella enterica and several bacterial species that produce a variety of fimbrial adhesions. During invasion by enteropathogenic protozoa, apicomplexan parasites utilize sialic acids...
Palavras-chave: Bacteria; Carbohydrate; Enteric pathogen; Infection; Protozoa; Virus.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4299

Efectos de la saponina, oleato de sodio y estearato de zinc sobre las bacterias rojas halofílicas
Provedor de dados: 20
Autores: Bertullo, V.H.; Perez Hettich, F.
Se estudiaron los efectos que la saponina, el oleato de sodio y el estearato de zinc producen sobre Pseudomonas salinarias, Halobacterium minutum, H. cutirubra, Sarcina littorallis y S. sreenivasani.
Tipo: Journal Contribution
Palavras-chave: Bacteria; Processed fishery products; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 1958
URL: http://hdl.handle.net/1834/3215

Die biologische Aktivität von Bakterien und Pilzen in zwei Bodenproben von der Isla de Salamanca (Kolumbien)
Provedor de dados: 20
Autores: Kunze, Ch.; Gnittke, J.
Se estudiaron las intensidades de la respiración y de la producción decatalasa de muestras de suelo de dos ditios ecológicamente diferentes de la Isla de Salamanca, bajo óptimas condiciones de incubación. Por medio de la inhibición de los bacterios con tetraciclina se puede diferenciar entre la actividad de los mismos y la de los hongos.
Tipo: Journal Contribution
Palavras-chave: Fungi; Bacteria; Fungi; Bacteria; Http://aims.fao.org/aos/agrovoc/c_3145; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 1973
URL: http://hdl.handle.net/1834/3306

Antarctic Psychrophile Bacteria Screening for Oil Degradation and Their Degrading Characteristics.
Provedor de dados: 20
Autores: Zheng, Zhou; Jin, Qing; Miao, Jinlai; Liu, Fangming.
Two hydrocarbon degrading bacteria NJ276 and NJ341 were screened from 385 Antarctic marine bacteria and their degrading characteristics were studied. Diesel oil as sole carbon source was used in this study. The results showed that the oil degradation rates of Antarctic psychrophile bacteria NJ276 and NJ341 were 23.47 % and 32.15 %, respectively, after 20 days culturation at 5 °C, and the rates were 43.95 % and 62.47 % respectively after 20 days culturation at 15 °C. The oil degradation abilities were enhanced remarkably with the increasing culture temperature. GC - MSs indicated the residual oil contained C15 - C21 7 alkyls after degradation by NJ276, and C16, C17 and C18 3 alkyls after degradation by NJ341. The 16S rDNA gene sequences homology and...
Tipo: Journal Contribution
Palavras-chave: Hydrocarbons; Degradation; Bacteria.
Ano: 2008
URL: http://hdl.handle.net/1834/5879

Capacité des moules dans la réduction des teneurs en nutriments et la charge bactérienne dans les effluents piscicoles
Provedor de dados: 20
Autores: Romdhane, M.S.; Chebil Ajjabi, L.; El Abed, O.
L’effluent des élevages de poissons renferme des concentrations plus élevées en nutriments dissous et en particules en suspension que celles présentes dans l’eau entrante. Cet état entraine par conséquent, des problèmes d’ordre environnementaux affectant les eaux côtières dues à l’eutrophisation et l’augmentation de la turbidité. Afin d’améliorer la qualité de l’effluent avant son déversement dans le milieu naturel, l’utilisation des bivalves comme filtre biologique est une alternative adéquate. Cette étude analyse la capacité de la moule Mytilus galloprovincialis de réduire les teneurs en sels nutritifs et la charge bactérienne dans les effluents de loups Dicentrarchus labrax en élevage. L’effluent issu des bassins de grossissement est pompé directement...
Tipo: Journal Contribution
Palavras-chave: Aquaculture effluents; Bacteria; Biofilters; Chlorophylls; Culture effects; Density; Effluents; Filtration; Fish; Mussel fisheries; Nitrogen; Nutrients (mineral); Phosphorus; Suspended particulate matter; Wastewater treatment; Water quality; Mytilus galloprovincialis; Wastewater treatment; Mussels; Nutrients; Http://aims.fao.org/aos/agrovoc/c_31155; Http://aims.fao.org/aos/agrovoc/c_25312; Http://aims.fao.org/aos/agrovoc/c_5010; Http://aims.fao.org/aos/agrovoc/c_5274.
Ano: 2008
URL: http://hdl.handle.net/1834/4154

Screening de l’activité anti-microfouling d’algues vertes récoltées sur la côte nord Tunisienne
Provedor de dados: 20
Autores: Chérif, W.; El Bour, M.; Daly Yahia-Kefi, O.
Les bio-salissures marines intéressent toute structure immergée en mer. Les dommages engendrés par ce phénomène sont économiquement importants. Les peintures utilisées pour prévenir du biofouling, utilisent des biocides organostaniques qui se sont avérés très toxiques et posent un problème environnemental important. Le présent travail s'intègre dans le cadre de la valorisation des macroalgues des côtes tunisiennes par la recherche de molécules naturelles antifouling pouvant se substituer aux substances déjà utilisées. Dans cette étude, nous avons analysé le potentiel anti-microfouling d’extraits organiques de 10 espèces d’algues vertes: Ulva linza, Ulva rigida, Chaetomorpha linum, Cladophora laetivirens, Codium bursa, Codium fragile, Caulerpa prolifera,...
Tipo: Journal Contribution
Palavras-chave: Algae; Seaweeds; Green algae; Bacteria.
Ano: 2011
URL: http://hdl.handle.net/1834/4741

Seasonal variation of antibacterial activity of the brown alga padina pavonica (l) thivy collected from northern coast of tunisia
Provedor de dados: 20
Autores: Ben Ali, A. I.; Ktari, L.; Boudabbous, A.; El Bour, M.
L’algue brune Padina pavonica a été récoltée durant une période d’une année (Juillet 2006 à Juin 2007), dans la côte nord de la Tunisie (Cap Zebib) en vue d'évaluer l'activité antibactérienne d’extraits bruts de l’algue et leur variabilité saisonnière. Les extraits d’algue ont été préparés en utilisant le  Dichlorométhane et le Dichlorométhane/Méthanol et testés périodiquement (tous les mois) sur une gamme de bactéries pathogènes et des bactéries associées isolées de l’algue. Une variation significative de l'activité antibactérienne liée au solvant d’extraction utilisé a été notée. Ainsi, les extraits au Dichloromethane/Méthanol sont plus actifs. Aussi nous avons noté la présence d’une variabilité saisonnière de l'activité antibactérienne. Ce sont les...
Tipo: Journal Contribution
Palavras-chave: Bacteria; Pathogenic bacteria; Seasonal variations; Antibacterials; Algae.
Ano: 2010
URL: http://hdl.handle.net/1834/4699

Efecto de las condiciones de transporte, recepción, aclimatación y siembra de Nauplios de Litopenaeus vannamei (Boone, 1931) sobre la sobrevivencia en larvicultura
Provedor de dados: 20
Autores: Suárez, J.A.; García, A.; Newmark, F.; Bador, R.
In order to identify initial factors that can affect shrimp postlarvae survival during larval rearing, three larval rearing facilities from the Caribbean coast of Colombia were samplesd for 71 nauplii batches during 10 months of 1996. For each batch, cualitative variables (origin, transportation mode, presence of bacteria in transport water) and quantitative variables (transportation time and density, physicochemical parameters of the water, proportion of each nauplii stage and of deformed nauplii) were monitored. Water quality and density (nauplii/l) variables were monitored during the whole process of nauplii packing, transport, reception, acclimatization and stocking. To evaluate the relationship between qualitative variables and larval rearing...
Tipo: Journal Contribution
Palavras-chave: Nauplii; Quality; Bacteria; Transport; Transport; Quality; Bacteria; Http://aims.fao.org/aos/agrovoc/c_7874; Http://aims.fao.org/aos/agrovoc/c_6400; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2001
URL: http://hdl.handle.net/1834/3112

Distribución de la biomasa de las fracciones planctónicas &lt; 200 μM y su contribución relativa a la biomasa total en las aguas oceánicas de la región noroccidental de Cuba.
Provedor de dados: 20
Autores: Orozco, M.V.; Loza, S.; Lugioyo, M.
Se evaluó el patrón de distribución espacial así como la contribución de las fracciones del plancton a la biomasa planctónica menor de 200 μm en la región NW de las aguas oceánicas adyacentes a Cuba en muestreos realizados en época de invierno (febrero de 1997 y marzo 1999). Los valores de la biomasa bacteriana variaron entre 1.6 y 11.4 mgC m-3, del nanoplancton entre 5.2 y 20.8 mgC m-3 (Xmedia = 11.4 ± 5.4 mgC m-3) y la del microzooplancton entre 4.2 y 15.5 mgC m-3 (Xmedia = 7.9 ± 3.2 mgC m-3). El microfitoplancton presentó valores de biomasa que variaron entre 1.95 y 15.7 mgC m-3 (Xmedia = 8.8 ± 3.7 mgC m-3). Los mayores valores de biomasa de todas las fracciones estudiadas se encontraron en las aguas oceánicas adyacentes a la zona entre las bahías de...
Tipo: Journal Contribution
Palavras-chave: 1461; Biomass; Bacteria; Nanoplankton; Oceanic waters; Zooplankton; Phytoplankton; Biomass; Bacteria.
Ano: 2009
URL: http://hdl.handle.net/1834/4119

Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements
Provedor de dados: 20
This manual describes the protocol approved by the international Scientific Steering Committee for the Joint Global Ocean Flux Study (JGOFS) for the most of the 20 JGOFS Core Measurements. However, the methods for the analysis of various parameters of the seawater CO2 system are described in a separate handbook. In order to have a complete set of the JGOFS measurements protocols, you should request a copy of the "Handbook of Methods for the Analysis of the Various Parameters of the Carbon Dioxide System in Seawater"version 2, A.G. Dickson and C. Goyet, eds. ORNL/CDIAC-74.
Tipo: Book
Palavras-chave: Oceanography; Ocean circulation; Oceanographic stations; Oceanographic data; Oceanographic surveys; Samples; Sampling; Data processing; Data; Data collections; Quality control; Salinity measurement; Oxygen; Inorganic carbon; Nitrites; Nitrates; Phosphorus compounds; Phosphorus cycle; Silicate minerals; Marine organisms; Microorganisms; Bacteriology; Bacteria; Zooplankton; Sampling; Nitrites; Nitrates; Zooplankton; Data processing; Data; Oxygen; Microorganisms; Bacteriology; Bacteria; Http://aims.fao.org/aos/agrovoc/c_6774; Http://aims.fao.org/aos/agrovoc/c_5190; Http://aims.fao.org/aos/agrovoc/c_5187; Http://aims.fao.org/aos/agrovoc/c_15490; Http://aims.fao.org/aos/agrovoc/c_10289; Http://aims.fao.org/aos/agrovoc/c_49816; Http://aims.fao.org/aos/agrovoc/c_5477; Http://aims.fao.org/aos/agrovoc/c_4807; Http://aims.fao.org/aos/agrovoc/c_768; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 1994
URL: http://hdl.handle.net/1834/2795

Distribución, relaciones tróficas y diversidad del bacterioplancton de las aguas oceánicas de Cuba
Provedor de dados: 20
Autores: Lugioyo Gallardo, G. M.
Tipo: Theses and Dissertations
Palavras-chave: Bacteria; Species diversity; Biodiversity; Bacteria; Species diversity; Biodiversity; Http://aims.fao.org/aos/agrovoc/c_765; Http://aims.fao.org/aos/agrovoc/c_33954; Http://aims.fao.org/aos/agrovoc/c_33949.
Ano: 2003
URL: http://hdl.handle.net/1834/3403

Distribución de coliformes fecales en el área marina de la costa ecuatoriana en las Provincias de Esmeraldas y Manabí, 2008-2013
Provedor de dados: 20
Autores: Palacios, C.
Here, we describe an evaluation of fecal coliform bacteria contamination state in the ecuadorian river side area in the Provinces of Esmeraldas and Manabí, between march and may from 2008 to 2013, from an n=109 data samples. From the results, it is pretty obvious the bacteria contamination in very specific places such as Esmeraldas's river mouth, Chone's river estuary and the opposite side of the main population from the studied areas such as Esmeraldas, La Chorrera in Perdenales, Bahía de Caráquez, Jaramijó and Manta. It provides an evaluation based on the maximum limit allowed of 200 NMP/100 ml for estuarine and sea waters as it's described on the chapter VI annex 1 of Texto Unificado de la Legislación Secundaria del Ministerio del Ambiente (TULSMA), as...
Tipo: Journal Contribution
Palavras-chave: Bacteria; Pollution; Pollution legislation.
Ano: 2013
URL: http://hdl.handle.net/1834/5751

Cyanobacterial occurrence and diversity in seagrass meadows in coastal Tanzania
Provedor de dados: 20
Autores: Lyimo, T.J.; Hamisi, M.I.; Muruke, M.H.S.
We report on the occurrence and diversity of cyanobacteria in intertidal seagrass meadows at Ocean Road and Mjimwema, Dar es Salaam, Tanzania. Nutrients, temperature and salinity were measured as comparative environmental factors. A total of 19 different cyanobacteria taxa were encountered, out of which eight were found exclusively in Mjimwema, four exclusively in Ocean Road and seven were common to both sites. Oscillatoria, Lyngbya and Spirulina were the dominant cyanobacterial genera. Cyanobacterial coverage was higher in Mjimwema (31–100%) than in Ocean Road (0–60%). The levels of nutrients in tidal pool waters at Ocean Road ranged from 0.45–1.03 ìmol NO3 -N/l, 0.19–0.27 ìmol NO2 -N/l and 0.03–0.09 ìmol PO4 -P/l. At Mjimwema the nutrient concentration...
Tipo: Journal Contribution
Palavras-chave: Sea grass; Salinity; Temperature; Bacteria; Nutrients; Salinity; Temperature; Http://aims.fao.org/aos/agrovoc/c_5274; Http://aims.fao.org/aos/agrovoc/c_6751; Http://aims.fao.org/aos/agrovoc/c_7657.
Ano: 2004
URL: http://hdl.handle.net/1834/1137

Caractérisation préliminaire à partir de la lagune de Bizerte (Tunisie) des bactéries tolérantes aux hydrocarbures aromatiques polycliques (HAPS)
Provedor de dados: 20
Autores: Duran, R.; El Bour, M.; Ben Said, O.; Goni, M. S.; Dalleli, M.; Aissa, P.
م إنجاز هذه الدراسة  لهدف عَزَلَ بَكْتِيريَا قادرة على استعمالتم إنجاز هذه الدراسة  لهدف عَزَلَ بَكْتِيريَا قادرة على استعمال الهيدروكربنات و مقاومة للمعادن والمضادات الحيوية و في هذا الإطار  تم أخذ عينات من الماء و التربة على مستوى  16محطة موزعة ببحيرة بنزرت. كما تم عزل البكتيريا بطريقة الإثراء  باستعمال وسط مائي معدني زائد هيدروكربنات (fluoranthène) وقد توصلنا في عملية أولية من عزل 130  من الأجذال المختلفة من البكتيريا والتي تم تعريفها عبر الخاصيات  الشكلية و البيوكيميائية. وأثبتت  النتائج الأولية أن البكتيريا القادرة على استعمال الهيدروكربنات تنتمي لمجموعات مختلفةGram – و  Gram+  على التَّوَالِي بنسبتي 65% و 35 %. وتضم مجموعة البكتيريا من نوع العصيات  Gram – أكبر عدد من الأجذال المعزولة (74 %). ختاما نشير أن دراسة قدرة الأجذال على مقاومة المعادن...
Tipo: Journal Contribution
Palavras-chave: Bacteria; Antibiotics; Biodegradation; Control resistance; Heavy metals; Hydrocarbons; Pollution monitoring; Antibiotics; Biodegradation; Heavy metals; Hydrocarbons; Http://aims.fao.org/aos/agrovoc/c_492; Http://aims.fao.org/aos/agrovoc/c_9261; Http://aims.fao.org/aos/agrovoc/c_2223; Http://aims.fao.org/aos/agrovoc/c_3719.
Ano: 2007
URL: http://hdl.handle.net/1834/4257

Etude de la résistance aux antibiotiques des bactéries isolées de "mytilus galloprovincialis.
Provedor de dados: 20
Autores: El Bour, M.; Bouamama, K.; El Abed, O.
L’étude des résistances vis-à-vis de différentes substances antibiotiques pour des espèces bactériennes (aérobies et anaérobies) isolées chez Mytilus galloprovincialis a été réalisée par la méthode standard de l’antibiogramme. Ainsi, 50 souches bactériennes aérobies et 20 souches anaérobies appartenant à plus de 25 espèces différentes ont été isolées et testées. Les résultats obtenus montrent des profils à plusieurs résistances vis-à-vis des différentes familles  d’antibiotiques testés. Les résultats obtenus révèlent des profils à 12 résistances différentes notamment chez l’espèce anaérobie (Propioni acnes). Aussi, chez l’espèce aérobie pré dominante ( Aeromonas hydrophila) nous avons mis en évidence au moins 6 antibiotypes différents. Ces résultats...
Tipo: Journal Contribution
Palavras-chave: Antibiotics; Bacterial diseases; Control resistance; Ecology; Eutrophication; Indicator species; Microbiological analysis; Bacteria; Mytilus galloprovincialis; Http://aims.fao.org/aos/agrovoc/c_765; Http://aims.fao.org/aos/agrovoc/c_31155.
Ano: 2006
URL: http://hdl.handle.net/1834/4266

Abundancia, actividad y diversidad de las bacterias heterótrofas en el Golfo de Batabanó y su uso como indicadoras ambientales
Provedor de dados: 20
Autores: Miravet Regalado, M.E.
Se caracterizó la abundancia, actividad y biodiversidad de las bacterias heterótrofas aeróbias en el Golfo de Batabanó en diferentes épocas del año, desde 1982 hasta el 2000 y su relación con algunos factores abióticos, con el fin de interpretar las variaciones que muestran estos parámetros microbiológicos ante los cambios ambientales de origen natural o antrópico. A escala espacial, las mayores concentraciones de bacterias heterótrofas (BH) en las aguas se registraron hacia la regiónW del golfo y las menores concentraciones hacia el E y el NE, en las aguas de la Ensenada de la Broa, relacionándose principalmente con la disponibilidad de materia orgánica lábil en las aguas y el efecto indirecto de la dirección y sentido principal de las corrientes marinas....
Tipo: Theses and Dissertations
Palavras-chave: Bacteria; Biodiversity; Bacteria; Biodiversity; Http://aims.fao.org/aos/agrovoc/c_765; Http://aims.fao.org/aos/agrovoc/c_33949.
Ano: 2003
URL: http://hdl.handle.net/1834/3398

Bacterias antárticas y agentes antibacterianos
Provedor de dados: 20
Autores: Domínguez, M.
La Antártica se ha constituido en una región de gran interés científico debido a las condiciones extremas que allí se presentan, en especial, de temperatura y radiación ultravioleta. En el campo de la microbiología, las investigaciones se han enfocado, principalmente, en el estudio de la  biodiversidad bacteriana, lo que ha llevado a la descripción de nuevas especies, y también gran interés ha despertado la producción de compuestos con aplicaciones  biotecnológicas. Entre éstos se encuentran los que pudieran presentar actividad antibacteriana, de gran importancia debido a que uno de los mayores problemas de los últimos años es la emergencia de patógenos intrahospitalarios con una amplia resistencia a los agentes antibacterianos disponibles, no existiendo...
Tipo: Journal Contribution
Palavras-chave: Bacteria; Biotechnology; Biotechnology; Bacteria; Http://aims.fao.org/aos/agrovoc/c_16165; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2008
URL: http://hdl.handle.net/1834/3372

Caracterización de cultivos  iniciadores en productos cárnicos. Parte 1
Provedor de dados: 20
Autores: Beldarraín, T.; Cepero, Y.; Bruselas, A.; Santos, R.; Ramos, M.; Moya, Y.; Núñez, M.; Vergara, N.
En este trabajo se caracterizaron tecnológicamente, a escala de laboratorio, 5 cultivos iniciadores. Las propiedades tecnológicas evaluadas fueron pH en caldo glucosado, viabilidad del cultivo, tolerancia a elevada concentración de sal, inactivación a 60 ºC por 30 min, entre otras. Todos los cultivos, por sus aptitudes acidificantes y población celular se consideraron aptos para su empleo industrial y se pudieron categorizar como productores de baja y alta acidez.
Tipo: Journal Contribution
Palavras-chave: Bacteria; Starters; Lactic acid bacteria; Microbial starters; Media production; Meat products.
Ano: 2008
URL: http://hdl.handle.net/1834/4896

Primeros registros de bacterias marinas heterótrofas y hongos aislados de los arrecifes que bordean la plataforma SW de Cuba.
Provedor de dados: 20
Autores: Miravet, M.E.; Enríquez, D.; Lugioyo, G.M.; Delgado, Y.; Núñez, R.; Cabrera, H.; Martí, J.
Se registran por primera vez para los arrecifes de la plataforma SW cubana, la presencia de 5 géneros y 16 especies de bacterias heterótrofas aeróbicas y 15 géneros de hongos, de los cuales, 7 se identificaron hasta especie. Los géneros Bacillus sp. (bacteria) y Aspergillus sp. (hongo) resultaron los más cosmopolitas de todos los géneros encontrados, predominando en las aguas y sedimentos de todos los arrecifes estudiados. Del total de cepas aisladas la mayor diversidad de géneros, tanto de bacterias heterótrofas aeróbicas como de hongos, correspondió a muestras procedentes del arrecife Juan García.
Tipo: Journal Contribution
Palavras-chave: New records; Species diversity; Coral reefs; Bacteria; Coral reefs; Species diversity; Bacteria; Http://aims.fao.org/aos/agrovoc/c_1880; Http://aims.fao.org/aos/agrovoc/c_33954; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2003
URL: http://hdl.handle.net/1834/3331

Contribución al estudio de las bacterias halofílicas en el mar ecuatoriano
Provedor de dados: 20
Autores: Villamar, F.
Durante los meses de marzo y abril de 1976, se realizó el estudio de las bacterias marinas en el mar ecuatoriano, las cuales sobrepasan densidades de 10 Bact./ml en el área océanica. Al mismo tiempo frente a las costas del Ecuador disminuyen estos valores entre 500 y 2.500 Bact./ml., excepto en el área del Golfo de Guayaquil, donde se encontró bacterias de origen continental en un gran parche de 106 bact./ml. de acuerdo al diagnóstico bioquímico diferencial, se trata de Bacilos Grammegativos semejantes a pseudomonas sp. y Alcalígenas sp. en la zona océanica y Micrococcus sp. frente al Golfo de Guayaquil. Se relacionan las bacterias halofílicas con la temperatura, oxígeno y salinidad superficial.
Tipo: Journal Contribution
Palavras-chave: Nannoplankton; Bacteria; Abundance; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 1987
URL: http://hdl.handle.net/1834/2139

Selección de bacterias con capacidad degradadora de hidrocarburos aisladas a partir de sedimentos del Caribe colombiano.
Provedor de dados: 20
Autores: Narváez-Florez, S.; Gómez, M.L.; Martínez, M.M.
A partir de sedimentos del Caribe colombiano se realizaron 31 aislamientos bacterianos en medio mínimo de sales suplementado con hidrocarburos (ACPM o petróleo crudo) como única fuente de carbono. Las cepas aisladas se sometieron a pruebas de selección en diferentes concentraciones de hidrocarburos y se escogieron once de ellas tolerantes al crudo y ACPM en un ámbito del 1-8% v/v. Posteriormente, con las cepas seleccionadas, se conformó un cultivo bacteriano mixto y se evaluó su capacidad de degradar hidrocarburos en un ensayo a escala de laboratorio, con una concentración del 2% v/v de ACPM en un periodo de 21 días. Mediciones de la biomasa en Unidades Formadoras de Colonias (UFC)/mL fueron empleadas para elaborar la curva de crecimiento del cultivo mixto...
Tipo: Journal Contribution
Palavras-chave: Biodegradation; Hydrocarbons; Bacteria; Crude oil; Biodegradation; Hydrocarbons; Bacteria; Crude oil; Http://aims.fao.org/aos/agrovoc/c_9261; Http://aims.fao.org/aos/agrovoc/c_3719; Http://aims.fao.org/aos/agrovoc/c_765; Http://aims.fao.org/aos/agrovoc/c_10178.
Ano: 2008
URL: http://hdl.handle.net/1834/3497

Biomasa y distribución espacial del bacterioplancton en el sector norte de la plataforma continental argentina (34º S-41º S)
Provedor de dados: 20
Autores: Hozbor, M.C.; Hernández, D.R.; Cucchi Colleoni, A.D.; Costagliola, M.C.; Peressutti, S.R.
The northern sector of the Argentine continental shelf (34º S-41º S) is a highly productive area that sustains important fishery resources like Argentine anchovy (Engraulis anchoita) and is characterized by the presence of frontal zones with retention of detritus and plankton. In marine environments the bacterioplankton represents one of the main components of the trophic nets and plays a key role in biogeochemical cycles. In this ecosystem biomass and bacterioplankton distribution as well as their relation to environmental variables were analyzed. The highest chlorophyll a values, not significantly related to bacterioplankton (p &gt; 0.05), were found in frontal and coastal areas. Abundance, bacterial biomass and cell volume values ranged 0.48-5.42 x 106...
Tipo: Journal Contribution
Palavras-chave: Picoplankton; Bacteria; Biomass; Fronts; Coastal zone; Abundance; Environmental factors; Geographical distribution.
Ano: 2013
URL: http://hdl.handle.net/1834/5498

Molecular genetic monitoring of bacterial communities in manzala lake, egypt, based on 16S rRNA gene analysis
Provedor de dados: 20
Autores: El Saied, H.E.
A first molecular genetic study on the diversity of bacterial communities at Manzala Lake, Egypt, was determined by culture-independent 16S rRNA gene analysis. Bulk DNAs were extracted from water and sediment at two different sampling sites namely; Bashtir and Genka, in the lake. The 16S rRNA gene was positively amplified by polymerase chain reaction (PCR) from bulk DNA of each sample, cloned and sequenced. The sequence analysis of one hundred clones from each clone library obtained number of phylotypes ranged from 8 phylotypes, in Genka water, to 20 phylotypes in each of the sediment samples. The 16S rRNA gene showed diversity in sediment samples higher than in water samples. Based on Libshuff pairwise clone library comparisons, the 16S rRNA gene...
Tipo: Journal Contribution
Palavras-chave: Field Study; Bacteria.
Ano: 2007
URL: http://hdl.handle.net/1834/2206

Caracterización y evaluación de la calidad del agua de la Bahía de Jaramijó-Provincia de Manabí durante el año 2008
Provedor de dados: 20
Autores: Rodriguez de Morán, A.
The current research was developed in Bahia of Jaramijó in the Province of Manabí during 2008, as part of the major project: " Characterization of the Ecuadorian Coast"  that has been developing by INOCAR, in order to contribute to the proper use of resources and the environment. It characterized and assesses the results obtained in the research of the quality of water of Bahía of Jaramijó, taking into consideration the averages and their behaviour in each level studied in two different season in the year (wet-dry) and in two tide condition (ebb and flow) of the different parameter physical, chemical and microbiological. During the research was established that coastal marine waters of Bahía of Jaramijó, not show pollution of organic origin neither...
Tipo: Journal Contribution
Palavras-chave: Water quality; Environmental legislation; Bacteria; Pollution; Oxygen.
Ano: 2013
URL: http://hdl.handle.net/1834/5758

Pseudomonas salinaria, agente productor del "Rojo" en los productos pesqueros salados
Provedor de dados: 20
Autores: Bertullo, V.H.
De acuerdo a las experiencias realizadas, encontramos que el agente causal del "rojo" es una bacteria cromogena, halofila que hemos clasificado como Pseudomonas salinaria siguiendo el criterio de Harrison y Kennedy.
Tipo: Journal Contribution
Palavras-chave: Cured products; Processed fishery products; Bacteria; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 1954
URL: http://hdl.handle.net/1834/3032

Leche fermentada de búfala con cultivos probióticos
Provedor de dados: 20
Autores: Barrera, Y.; Real, E.; Ortega, O.
El objetivo de este trabajo fue definir la tecnología para elaborar una leche fermentada de búfala con buena aceptación y adecuada viabilidad. Se estableció el efecto del cultivo asociado de bacterias probióticas de Lactobacillus acidophilus + Lactobacillus casei, sobre la capacidad de acidificación, firmeza y características sensoriales de una leche fermentada de limón con una proporción de 1,5 % de cada cultivo. Se tomó como control el yogurt de búfala elaborado con 3 % del cultivo tradicional. Fue determinada la capacidad acidificante del cultivo mixto en la leche, la composición físico-química del producto, viabilidad, calidad microbiológica, así como su durabilidad a 4 ± 2 °C. En cuanto a la capacidad acidificante del cultivo mixto no fueron...
Tipo: Journal Contribution
Palavras-chave: Bacteria; Technology; Shelf life; Fermented milk; Lactobacillus casei; Lactobacillus acidophilus.
Ano: 2008
URL: http://hdl.handle.net/1834/4844

Probióticos como herramienta biotecnológica en el cultivo de camarón: reseña
Provedor de dados: 20
Autores: Villamil-Díaz, L.; Martínez-Silva, M.A.
Las bacterias probióticas se definen como microorganismos vivios que administrados como suplemento en la dieta pueden causar modificaciones en la microbiota asociada al tracto gastrointestinal del hospedador y generar efectos benéficos como el cincremento en la conversión alimantaria, en la resistencia a enfermedades y de la calidad del agua. Durante la última década, su aplicación en el cultivo de camarón se ha hecho frecuente ya que han rugido varios productos comerciales ideados para este fin. Al mismo tiempo, aunque hay publicados varios artículos científicos en el tema, es evidente que hace falta orientar los estudios para entender los mecanismos de acción de los probióticos, así como para establecer los protocolos de aplicación, teniendo en cuenta...
Tipo: Journal Contribution
Palavras-chave: Bacteria; Shrimp culture; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2009
URL: http://hdl.handle.net/1834/3615

Contribuciones a la mineralización de materiales orgánicos en la Isla de Salamanca
Provedor de dados: 20
Autores: Steubing, L.
Los procesos de mineralización del carbono y del nitrógeno fueron estudiados en un terraplén costero, en una hondonada y en un terraplén interno de la Isla de Salamanca en el Mar Caribe. El pH, y el contenido de cloruros y carbono, lo mismo que la capacidad hídrica fueron los más altos en el suelo húmedo de la hondonada; seguido del terraplén interno; el terraplén costero tuvo la capacidad hídrica más deficiente, y el más bajo contenido en cloruros y en nitrógeno. En la hondonada se encontró el más alto contenido en bacterias; las mayores poblaciones de hongos se encontraron en el terraplén interno. La actividad del suelo (producción de CO2 y amonificación) dependió del contenido de nitrógeno y cloruros de los lugares investigados.
Tipo: Journal Contribution
Palavras-chave: Coastal lagoons; Estuaries; Geology; Soils; Bacteria; Estuaries; Geology; Coastal lagoons; Bacteria; Http://aims.fao.org/aos/agrovoc/c_2673; Http://aims.fao.org/aos/agrovoc/c_3232; Http://aims.fao.org/aos/agrovoc/c_1697; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 1971
URL: http://hdl.handle.net/1834/3248

Capacité des moules dans la réduction des teneurs en nutriments et la charge bactérienne dans les effluents piscicoles
Provedor de dados: 20
Autores: Chebil Ajjabi, L.; Romdhane, M.S.; El Abed, A.
L’effluent des élevages de poissons renferme des concentrations plus élevées en nutriments dissous et en particules en suspension que celles présentes dans l’eau entrante. Cet état entraine par conséquent, des problèmes d’ordre environnementaux affectant les eaux côtières dues à l’eutrophisation et l’augmentation de la turbidité. Afin d’améliorer la qualité de l’effluent avant son déversement dans le milieu naturel, l’utilisation des bivalves comme filtre biologique est une alternative adéquate. Cette étude analyse la capacité de la moule Mytilus galloprovincialis de réduire les teneurs en sels nutritifs et la charge bactérienne dans les effluents de loups Dicentrarchus labrax en élevage. L’effluent issu des bassins de grossissement est pompé directement...
Tipo: Journal Contribution
Palavras-chave: Aquaculture effluents; Bacteria; Biofilters; Chlorophylls; Chlorophylls; Culture effects; Density; Effluents; Filtration; Fish; Mussel culture; Nitrogen; Nutrients (mineral); Phosphorus; Suspended particulate matter; Wastewater recycling; Wastewater treatment; Water quality; Fish; Nitrogen; Phosphorus; Water quality; Mussels; Nutrients; Bacteria; Chlorophylls; Density; Effluents; Filtration; Wastewater treatment.
Ano: 2008
URL: http://hdl.handle.net/1834/3756

Actividad biológica en bacterias heterótrofas procedentes de "Bahía de La Habana".
Provedor de dados: 20
Autores: Ortiz, E.; Morales, M.; Cartaya, Y.; Miranda, A.; Fonseca, E.; Paneque, K.; Caballero, V.; Martínez, C.; Díaz, Y.
En el presente trabajo se evaluó la actividad biológica de 87 aislamientos de bacterias heterótrofas procedentes de la "Bahía de La Habana". El espectro de potencialidades mostró que el 29,9% de los mismos presentó actividad antimicrobiana frente a las cepas indicadoras empleadas. Un 63,2% presentó actividad caseinasa, el 44,8% mostró su actividad hemolítica y el 83,4% fue capaz de excretar compuestos con actividad superficial. Respecto a los mecanismos degradativos, el 57,5% de ellas, mostró preferencia por el petróleo como fuente de carbono y el 42,5% por los compuestos organoclorados. Se evidenció la amplia gama de actividades con Bioactividad de bacterias heterótrofas aplicación biotecnológica que poseen las bacterias heterótrofas aisladas de éste...
Tipo: Preprint
Palavras-chave: Biological collections; Geographical isolation; Bacteria; Heterotrophic organisms; Bacteria; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2004
URL: http://hdl.handle.net/1834/3655

Bacterias antárticas y agentes antibacterianos
Provedor de dados: 20
Autores: Dominguez, M.
La Antártica se ha constituido en una región de gran interés científico debido a las condiciones extremas que allí se presentan, en especial, de temperatura y radiación ultravioleta. En el campo de la microbiología, las investigaciones se han enfocado, principalmente, en el estudio de la biodiversidad bacteriana, lo que ha llevado a la descripción de nuevas especies, y también gran interés ha despertado la producción de compuestos con aplicaciones biotecnológicas. Entre éstos se encuentran los que pudieran presentar actividad antibacteriana, de gran importancia debido a que uno de los mayores problemas de los últimos años es la emergencia de patógenos intrahospitalarios con una amplia resistencia a los agentes antibacterianos disponibles, no existiendo...
Tipo: Journal Contribution
Palavras-chave: Bacteria; Antibiotics; Antibiotics; Bacteria; Http://aims.fao.org/aos/agrovoc/c_492; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2009
URL: http://hdl.handle.net/1834/3365

Groundnut bacterial wilt
Provedor de dados: 30
Autores: V.K.
Palavras-chave: Groundnuts; Pathogens; Bacteria; Germplasm; Cultural control; Diseases; Pilot farms; Potatoes; Genotypes; Sweet potatoes.
Ano: 1993
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4177

Stability analysis of chickpea large genomic DNA inserts in Agrobacterium
Provedor de dados: 30
Autores: Rajesh, P.N.
Palavras-chave: Cloning; Genomes; Plasmids; DNA; Chickpeas; Genetic transformation; Chromosomes; Antibiotics; Bacteria; Genes.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3293

Field diagnosis of groundnut diseases
Provedor de dados: 30
Autores: P.
Palavras-chave: Fungi; Groundnuts; Necrosis; Fruits; Diseases; Mycoplasma; Viruses; Bacteria; Environmental factors.
Ano: 1992
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4249

Groundnut bacterial wilt in Asia
Provedor de dados: 30
Autores: S.L.
Palavras-chave: Crops; Groundnuts; Genotypes; Bacteria; Germplasm; Breeds (animals); Pathogens; Yield losses; Electrophoresis.
Ano: 1998
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4204

Groundnut bacterial wilt: past, present, and future
Provedor de dados: 30
Autores: V.K.
Palavras-chave: Bacteria; Groundnuts; Pathogens; PCR; DNA; Enzymes; Genotypes; Oil crops; Mutants.
Ano: 1994
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4181

Combined application of Pseudomonas fluorescens and Trichoderma viride has an improved biocontrol activity against stem rot in groundnut
Provedor de dados: 30
Autores: Manjula, K.
Palavras-chave: Biological control; Biochemical compounds; Bacteria; Poultry equipment; Fungi; Enzymes; Fungicides; Antibiotics; Seed treatment; Chitin.
Ano: 2004
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3322

Nitrogen Nutrition of Groundnut in Alfisols
Provedor de dados: 30
Autores: P.T.C.
Palavras-chave: Root nodulation; Groundnuts; Nitrogen fixation; Genotypes; Rhizobium; Bacteria; Nitrogen; Enzymes; Application methods; Legumes.
Ano: 1990
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4084

Linking estuarine nematodes to their suspected food: a case study from the Westerschelde estuary (south-west Netherlands)
Provedor de dados: 27
Autores: Moens, T.; Van Gansbeke, D.; Vincx, M.
The present study investigates correlations between abundances of nematodes (at the genus level) and benthic microalgae on an intertidal mudflat in the Westerschelde Estuary (south-west Netherlands), using both multi- and univariate methods. Two sample series, covering surface areas of 10 cm(2) (meioscale) and 1.25 cm(2) (microscale) per sample were analysed. Trophic type analysis indicated that an average of 31% of the nematode community were candidate grazers of microalgae. Multivariate data analysis indicated that only a limited part of the variation in the nematode data could be explained in relation to pigments. Total nematodes did not show any correlation with the pigment data. On the meioscale, the genera &lt;i&gt;Tripyloides&lt;/i&gt; and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecology; Marine nematodes; Meiobenthos; Populations; Populations; Scale; Scale; Bacteria.
Ano: 1999
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=7332

Search for beneficial bacterial strains for turbot (&lt;i&gt;Scophthalmus maximus&lt;/i&gt; L.) larviculture
Provedor de dados: 27
Autores: Huys, L.; Dhert, P.; Robles, R.; Ollevier, F.P.; Sorgeloos, P.; Swings, J.
The aerobic bacterial flora in the gut of turbot larvae and their influence on larval survival was examined. Two turbot experiments were run with six replicates each time. Large variation, from 0% up to 44%, was observed in the survival percentage of turbot larvae. There was no correlation between the number of bacteria present in the gut of turbot larvae and the larval survival rate. During both experiments, all replicates followed nearly the same rate of bacterial development in the gut of turbot larvae going from circa 10&lt;sup&gt;2&lt;/sup&gt; CFU larva&lt;sup&gt;-1&lt;/sup&gt; just before first feeding at day 3 post hatch to 10&lt;sup&gt;5&lt;/sup&gt; CFU larva&lt;sup&gt;-1&lt;/sup&gt; at day 9 post hatch.In total, 127 bacterial isolates from 12...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aerobic bacteria; Aquaculture; Commensals; Digestive system; Fish culture; Fish larvae; Gastrointestinal tract; Microorganisms; Rearing; Scophthalmus maximus; Survival; Bacteria; Scophthalmus maximus; Vibrio mediterranei.
Ano: 2001
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=5720

Different benthic size-compartments and their relationship to sediment chemistry in the deep Eurasian Arctic Ocean
Provedor de dados: 27
Autores: Kröncke, I.; Vanreusel, A.; Vincx, M.; Wollenburg, J.; Mackensen, A.; Liebezeit, G.; Behrends, B.
During the Arctic Expedition ARK VIII/3 (August to September 1991) with &lt;i&gt;RV Polarstern&lt;/i&gt;, the macrofauna, meiofauna, Foraminifera, bacteria were sampled and sediment chemistry was determined at 13 stations along a transect from the Barents Sea slope across the deep Arctic Eurasian Basins towards the Lomonosov Ridge. Water depths ranged from 258 to 4427 m. In general, higher values for all benthic compartments as well as total organic carbon (TOC) and total hydrolysable amino acids (THAA) were recorded for the Barents Sea slope than for the deeper stations in the basins and the ridge slopes. The only significant correlation found was between macrofaunal abundance and depth. Bacterial and all faunal abundances as well as bacterial and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Foraminifera [hole bearers].
Ano: 2000
URL: http://www.vliz.be/imisdocs/publications/273306.pdf

Selected bacterial strains protect &lt;i&gt;Artemia&lt;/i&gt; spp. from the pathogenic effects of &lt;i&gt;Vibrio proteolyticus&lt;/i&gt; CW8T2
Provedor de dados: 27
Autores: Verschuere, L.; Heang, H.; Criel, G.R.J.; Sorgeloos, P.; Verstraete, W.
In this study &lt;i&gt;Vibrio proteolyticus&lt;/i&gt; CW8T2 has been identified as a virulent pathogen for &lt;i&gt;Artemia&lt;/i&gt; spp. Its infection route has been visualized with transmission electron microscopy. The pathogen affected microvilli and gut epithelial cells, disrupted epithelial cell junctions, and reached the body cavity, where it devastated cells and tissues. In vivo antagonism tests showed that pre-emptive colonization of the culture water with nine selected bacterial strains protected &lt;i&gt;Artemia&lt;/i&gt; juveniles against the pathogenic effects. Two categories of the selected strains could be distinguished: (i) strains providing total protection, as no mortality occurred 2 days after the experimental infection with &lt;i&gt;V....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic animals; Bacteria; Biological control; Brine shrimp culture; Disease control; Infectious diseases; Microbiological analysis; Pathogenic bacteria; Artemia; Vibrio proteolyticus.
Ano: 2000
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=45

Selective attraction of marine bacterivorous nematodes to their bacterial food
Provedor de dados: 27
Autores: Moens, T.; Verbeeck, L.; de Mayer, A.; Swings, J.; Vincx, M.
This paper explores the role of selective attraction to food in determining the spatial (micro)distribution of closely related nematode species. The attractiveness of 3 different bacterial strains to 4 species of Monhysteridae, &lt;i&gt;Diplolaimelloides meyli, Diplolaimella dievengatensis, Monhystera&lt;/i&gt; sp. and &lt;i&gt;Geomonhystera disjuncta&lt;/i&gt;, was studied in a multiple choice design. In our study area, the 4 nematode species considered are associated with &lt;i&gt;Spartina anglica&lt;/i&gt; detritus decay and have partially overlapping microhabitat preferences. As they all belong to the same feeding guild, they are potential competitors for food. Each of the 4 nematode species was attracted to the bacterial strain B1, but important...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemotaxis; Recruitment; Taxis; Bacteria; Diplolaimella dievengatensis; Diplolaimelloides meyli; Geomonhystera disjuncta; Nematoda [Nematodes]; Spartina anglica [common cord-grass].
Ano: 1999
URL: http://www.vliz.be/imisdocs/publications/273317.pdf

Promotion of growth and control of damping-off (Rhizoctonia solani) of greenhouse tomatoes amended with vermicompost
Provedor de dados: 85
Autores: Rivera,MC; Wright,ER; López,MV; Garda,D; Barragué,MY.
The pathogen Rhizoctonia solani (teleomorph Tanatephorus cucumeris) can affect tomatoes germination and emergence and cause basal rot of seedlings. It is generally accepted that composts suppress plant diseases, improve soil nutrient availability and stimulate plant growth. However, no reports have been found on the simultaneous evaluation of vermicompost as plant growth promoter and suppressive to damping-off caused by R. solani on tomatoes. This research evaluated the suppressive effects of different concentrations of vermicompost against R. solani and the ability of vermicompost to promote tomato seedlings growth. The microbial composition of the substratum was explored. Thirty six microorganisms were isolated, 13 of which were antagonic to R.solani in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antagonism; Bacteria; Biological control; Disease incidence; Fungi.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572004000100028

ASSESSMENT OF JEDDAH PARKS HEALTH STATUS BY Cyperus rotundus AND SOIL ANALYSIS UNDER WASTE WATER IRRIGATION
Provedor de dados: 87
Autores: EL-NAKHLAWY,F.S.; AL-SHAREEF,A.R.; IHSAN,M.Z.
ABSTRACT To investigate the health status of public parks in Jeddah city, a field survey was conducted based on the Cyperus rotundus weed and soil analysis of wastewater-irrigated parks during 2012. The city was divided into five different locations (North, South, East, West and Middle) and five parks were randomly sampled from each location; thus, the total number of sampled parks were twenty five. Toxic metals concentration were analyzed in the soil and in the plants while bacterial count was determined from the weed leaves. All studied toxic metals have significantly crossed the European Union safety limits for plant samples, while soil samples were just beyond the limit. The highest concentration was perceived for Lead (133-232 mg kg-1) and was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyperus rotundus; Chromium; Bacteria; Parks health; Waste water irrigation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100213

Tarragon (Artemisia Dracunculus L.) Could Alleviate Negative Effects of Stocking Density in Laying Hens
Provedor de dados: 89
Autores: Kaya,H; Karaalp,M; Kaynar,Ö; Tekçe,E; Aksakal,A; Bayram,B.
ABSTRACT The study was conducted to determine the effects of stocking density (SD, 5 or 7 layers/cage) and tarragon (Artemisia dracunculus L.) diet supplementation at four levels (0, 1.2, 6 and 12 g/kg feed) on performance, certain egg characteristics, serum, liver, egg yolk and small intestine bacteria parameters in laying hens. The experiment was carried out over a period of 8 weeks, with 192 Lohman Brown commercial hybrids at 50-w-age. The results showed that an increased SD reduced feed intake (FI; p&lt;0.01) and egg production (p&lt;0.05), but had no effect on the weight gain, feed conversion ratio, damaged egg ratio, egg weight and egg quality (p&gt;0.05) parameters. The supplementation of tarragon to the diet reduced the FI and damaged egg ratio...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Bacteria; IgG; Stocking density; Tarragon.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100301

Decontamination of naturally contaminated liquid nitrogen storage tanks
Provedor de dados: 96
Autores: Pessoa,Gilson Antonio; Rubin,Mara Iolanda Batistella; Silva,Carlos Antonio Mondino; Rosa,Denize Costa da.
The objective of this study was to evaluate the efficacy of cleaning and decontamination procedures in liquid nitrogen tanks. We evaluated 151 canisters and 133 bottoms from 133 nitrogen tanks of companies or farms for the presence of bacteria and fungi. Samples were collected from the canisters and the bottom of tanks containing liquid nitrogen. Tanks were divided into Group 1 (G1): tanks decontaminated with 2% glutaraldehyde - Glutaron® II (n = 16 canisters in 8 tanks); Group 2 (G2): decontamination with 70% ethanol (n = 20 canisters in 10 tanks); and Group 3 (G3): decontamination with 70% ethanol (n = 115 canisters in 115 tanks). Tanks in Groups 1 and 2 belonged to companies; Group 3 tanks belonged to farms. The culture of canisters showed twelve genera...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artificial insemination; Bacteria; Fungi; Semen.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000500244

Molecular evolution in bacteria: cell division
Provedor de dados: 59
Autores: Trevors,J.T.
Molecular evolution in bacteria is examined with an emphasis on the self-assembly of cells capable of primitive division and growth during early molecular evolution. Also, the possibility that some type of encapsulation structure preceeded biochemical pathways and the assembly of genetic material is examined. These aspects will be considered from an evolutionary perspective.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell division; Molecular evolution; Bacteria; Self-assembly.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400001

Bacteria and protozoa populations in groundwater in landfill area in São Carlos, SP
Provedor de dados: 59
Autores: Fusconi,Roberta; Godinho,Mirna Januária Leal.
The microbial populations of groundwaters were analyzed in a region under the influence of a landfill (piezometer L12) in the town of São Carlos, São Paulo, Brazil, and in an area not influenced by the landfill (piezometer L5). Heterotrophic bacteria were counted by spread plate method and the number of protozoa was estimated by the most probable number method. There was a larger number of organisms in well L12, with a mean value of 15.76 x 104 CFU/ml for bacteria and 9.7 MPN/ml for protozoa, whereas the mean values for piezometer L5 were 2.88 x 104 CFU/ml for bacteria and 3.4 MPN/ml for protozoa. The greater abundance detected in piezometer L12 may be related to the influence of the leachate through the landfill on the microbial populations, also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Protozoa; Groundwater; Landfill.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300003

Cinnabarin synthesis by Pycnoporus sanguineus strains and antimicrobial activity against bacteria from food products
Provedor de dados: 59
Autores: Smânia,Elza de Fátima Albino; Smânia Júnior,Artur; Loguercio-Leite,Clarice.
Among three strains of Pycnoporus sanguineus, MIP 89007 produced more cinnabarin than MIP 95001 and MIP 95002. The antimicrobial activity of cinnabarin was tested against 11 species of bacteria isolated from food. Bacillus cereus and Leuconostoc plantarum were the most sensitive to cinnabarin, being inhibited by 0.0625 mg/ml. Klebsiella pneumoniae was the least sensitive (&amp;gt;4.0 mg/ml).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pycnoporus sanguineus; Cinnabarin; Antimicrobial activity; Fungus; Bacteria.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400017

MICROBIAL COUNTS OF DARK RED LATOSOL SAMPLES STORED AT DIFFERENT TEMPERATURES
Provedor de dados: 59
Autores: Vieira,Francisco Cleber Sousa; Nahas,Ely.
The number of colony forming units (CFU) of different groups of bacteria and fungi in samples stored at temperatures of 5 to -12oC for 0-32 weeks was evaluated. The number of CFU obtained after the different periods of storage of red latossol soil was compared with the number of colonies obtained immediately after removal of soil samples (time zero). The number of total bacteria and actinomycetes in the samples remained practically unchanged throughout the storage period. The number of Gram-negative bacteria decreased by as much as 69% compared to control, while the number of Bacillus spp and of fungi increased 1.9 to 4.9 times starting from the 12th week in samples stored at 5oC. Except for the variations observed in fungal counts, the remaining groups of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Fungi; Phosphatase; Soil storage.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300001

Fermentation and epiphytic microflora dynamics in sugar cane silage
Provedor de dados: 63
Autores: Pedroso,André de Faria; Nussio,Luiz Gustavo; Paziani,Solidete de Fátima; Loures,Daniele Rebouças Santana; Igarasi,Mauricio Scoton; Coelho,Rodrigo Michelini; Packer,Irineu Humberto; Horii,Jorge; Gomes,Luiz Humberto.
Sugar cane silages are characterized by extensive yeast activity, alcohol production and great dry matter - DM - losses. Better knowledge of the fermentation process is fundamental to the development of efficient ensilage techniques for this forage. This study evaluates temporal changes in chemical composition, DM losses and epiphytic microflora in sugar cane silage. Mature sugar cane, variety RB835486 (12 months of vegetative growth), was hand harvested, processed in a stationary chopper and ensiled in 20-L plastic buckets provided with valves for gas release and a device for effluent collection. Laboratory silos were kept at ambient temperature and sampled after ½, 1, 2, 3, 7, 15, 45, 90, 120 and 180 days. Ethanol concentration reached 6.4% in DM after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Water soluble carbohydrates; Losses; Yeasts; Bacteria.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000500003

Fermentation of irradiated sugarcane must
Provedor de dados: 63
Autores: Alcarde,André Ricardo; Walder,Júlio Marcos Melges; Horii,Jorge.
Bacillus and Lactobacillus are bacteria that usually contaminate the ethanolic fermentation by yeasts and may influence yeast viability. As microorganisms can be killed by ionizing radiation, the efficacy of gamma radiation in reducing the population of certain contaminating bacteria from sugarcane must was examined and, as a consequence, the beneficial effect of lethal doses of radiation on some parameters of yeast-based ethanolic fermentation was verified. Must from sugarcane juice was inoculated with bacteria of the genera Bacillus and Lactobacillus. The contaminated must was irradiated with 2.0, 4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation. After ethanolic fermentation by the yeast (Saccharomyces cerevisiae) the total and volatile acidity produced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Gamma radiation; Yeast.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400011

Recovery of soil microbiological properties in a degraded area planted with Corymbia citriodora and Leucaena leucocephala
Provedor de dados: 63
Autores: Valpassos,Maria Alexandra Reis; Maltoni,Kátia Luciene; Cassiolato,Ana Maria Rodrigues; Nahas,Ely.
The influence of reforestation was evaluated during two season periods (February and October) in sites planted with Corymbia citriodora and Leucaena_leucocephala through microorganism counts (bacteria, fungi, actinomycetes, and nitrite oxidizers) and microbial activity (respiratory and urease activities). An Atlantic forest and a bare soil site were used as controls. The general trends of the variables in the different soils were: Atlantic forest or L. leucocephala &gt; C. citriodora &gt; bare soil. The microbial populations in L. leucocephala and C. citriodora soils were significantly higher (P &lt; 0.05) than that found in the bare soil. Similar results were obtained for respiratory and urease activities. The microbiological variables of the soil under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Fungi; Forest degradation; Respiratory activity; Urease.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000100010

Fermentative and microbiological profile of marandu-grass ensiled with citrus pulp pellets
Provedor de dados: 63
Autores: Bernardes,Thiago Fernandes; Reis,Ricardo Andrade; Moreira,Andréia Luciane.
High-wet roughages with low content of soluble carbohydrates, such as tropical grasses, should be inappropriate for producing silages of adequate quality. This study aimed to evaluate the fermentative and microbiological profiles of Marandu-grass ensilaged with pelleted citric pulp (PCP). Brachiaria brizantha (Hochst ex. A. Rich) Stapf cv. Marandu with 58 days of vegetative growth was harvested for producing experimental silages in PVC silos provided with Bünsen valves and density capacity of 900 kg m-3. Treatments were three PCP levels (0, 50 and 100 g kg-1 of fresh forage) and seven times (1, 4, 7, 14, 21, 28 and 56 days) after sealing. PCP inclusion increased soluble carbohydrate concentrations by 15 to 20% and reduced pH (5.3 to 4.2) and N-NH3 levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additive; Bacteria; Fermentation; Tropical grass; Silage.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000300003

การแยกเชื้อและลักษณะของเชื้อ Azospirillum จากรากข้าว
Provedor de dados: 106
Autores: Jarunee Mejuice; Naruemon Uthakur; Savit Mejuice.
Azospirillum is consider to be plant-growth promoting bacteria (PGPR) associated with rice and stimulate plant growth by promoting nutrition by the process of biological nitrogen fixation. The aim of this study was to isolate and characterise of Azospirillum from root rice varieties. The morphology, physiology and biochemistry properties of the bacteria were tested. The result showed that the bacterial isolates of Azospirillum from three rice varieties; Khao Dawk Mali 105, Sew Mae Jan and RD 6 were Azospirillum brasilense and Azospirillum amazonense. The combined properties of the two species were discussed to be used for beneficial application of microbial fertilizer. 
Tipo: Collection
Palavras-chave: Rice diseases; Characterizing; Bacteria; Rice root; การจำแนกลักษณะเชื้อ; โรคข้าว; เชื้อแบคทีเรีย; ข้าว; รากข้าว.
Ano: 2012
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5406

GARRAPATAS DURAS (ACARI: IXODIDAE) DE COLOMBIA, UNA REVISIÓN A SU CONOCIMIENTO EN EL PAÍS
Provedor de dados: 37
Autores: ACEVEDO-GUTIÉRREZ,Leidy Yoana; PATERNINA,Luis Enrique; PÉREZ-PÉREZ,Juan Camilo; LONDOÑO,Andrés Felipe; LÓPEZ,Gustavo; RODAS,Juan David.
RESUMEN Las garrapatas son artrópodos de gran impacto sobre la economía pecuaria y la salud humana debido a las pérdidas generadas en la producción, y a su capacidad de transmitir microorganismos. Pese a la evidencia de circulación de una gran diversidad de garrapatas en Colombia, no se cuenta con compilaciones actualizadas de la variedad de especies presentes, por lo cual los autores presentan una revisión de literatura sobre las garrapatas de la familia Ixodidae reportadas en Colombia incluyendo su distribución geográfica, sus hospederos y los microorganismos reportados en aquellas especies registradas en este país. En la recopilación se evidenció la presencia en Colombia de diferentes especies de garrapatas de los géneros Amblyomma, Ixodes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artrópodos vectores; Artrópodos hematófagos; Bacteria; Distribución espacial; Infestación; Virus.
Ano: 2020
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000100126

DIVERSIDAD DE BACTERIAS ENDÓFITAS ASOCIADASA RAÍCES DEL PASTO COLOSUANA (Bothriochloa pertusa)EN TRES LOCALIDADES DEL DEPARTAMENTODE SUCRE, COLOMBIA.
Provedor de dados: 37
Autores: PEREZ C,ALEXANDER; ROJAS S,JOHANNA; FUENTES C,JUSTO.
El objetivo del presente trabajo fue aislar diversidad de bacterias endófitas cultivables asociadas a raíces del pasto colosuana Bothriochloa pertusa (L) A. Camus en tres localidades del departamento de Sucre, Colombia. La diversidad de bacterias endófitas fue realizada mediante el aislamiento de colonias en medios de cultivos. La densidad poblacional fue estimada por conteo directo de colonias en placa y las características culturales de cada morfotipo fueron obtenidas mediante observación de cada colonia formada. Se determinó la correlación diversidad, densidad poblacional y localidades, utilizando ANOVA y análisis de componentes principales o de correspondencia simple, mediante el programa estadístico R, 2009 (4,5). Fueron muestreadas 20 fincas por...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Endófitas; Raíz; Pasto.
Ano: 2010
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000200015

IDENTIFICACIÓN MOLECULAR DE POBLACIONES BACTERIANAS ASOCIADAS AL CARACOL PALA (Strombus gigas) DEL CARIBE COLOMBIANO
Provedor de dados: 37
Autores: ACOSTA,EDINSON ANDRÉS; GÓMEZ,ELIANA; ROMERO TABAREZ,MAGALLY; CADAVID RESTREPO,GLORIA ESTER; MORENO HERRERA,CLAUDIA XIMENA.
El caracol Pala, Strombus gigas (Strombidae), es de gran importancia ecológica y socioeconómica en el área caribeña colombiana. Sin embargo, es una especie catalogada como "vulnerable" y existe muy poca información referente a las especies bacterianas asociadas al caracol que puedan ser importantes para el desarrollo, manejo productivo y de seguridad acuícola de estos gastrópodos. En este trabajo, nosotros empleamos un estudio microbiológico y molecular de la región intergénica entre los genes 16S y 23S rDNA, análisis del gen rDNA 16S y secuenciación, para analizar las bacterias asociadas al caracol Pala (S. gigas). La composición de bacterias cultivables asociadas fue evaluada por su capacidad para crecer en agar marino y en medios de cultivos selectivos....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strombus gigas; Caracol pala; Bacteria; Región intergénica 16S-23S; RDNA 16S.
Ano: 2009
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2009000200007

Influence of seasonality and production method on the antibacterial activity of propolis
Provedor de dados: 2
Autores: Souza, Edison Antonio de; Universidade Estadual Paulista “Júlio de Mesquista Filho"; Inoue, Hemily Tiemi; Universidade Estadual Paulista “Júlio de Mesquista Filho"; Fernandes Júnior, Ary; Universidade Estadual Paulista “Júlio de Mesquista Filho"; Veiga, Nabor; Universidade Estadual Paulista “Júlio de Mesquista Filho"; Orsi, Ricardo de Oliveira; UNESP - Botucat - SP.
The antibacterial activity of propolis produced throughout the year by different methods of collection (‘intelligent’ collector of propolis - ICP; plastic screen - PC; conventional scraping - CS) on Staphylococcos aureus and Escherichia coli is investigated. Fifteen beehives (five per collector) of Africanized Apis mellifera were used. Monthly produced propolis, with the same collection technique, was mixed for the preparation of the extract. The ethanol extract of propolis (EEP) was prepared at the ratio of 30% (30 g of propolis, completing the volume for 100 mL with ethanol 70%). Two microorganisms, a positive bacterium Gram Staphylococcus aureus and a negative bacterium Gram Escherichia coli, through the methodology of diffusion in agar, were used for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apiculture; Biological properties; Quality; Bacteria.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/21436

Propolis extract and sodium monensin on ruminal fermentation and hematological parameters in sheep
Provedor de dados: 2
Autores: Silva, Fernanda Gomes Bezerra da; Yamamoto, Sandra Mari; Silva, Eva Mônica Sarmento da; Queiroz, Mario Adriano Ávila; Gordiano, Layse Araújo; Formiga, Marcela Almeida.
This study evaluated the effects of different concentrations of 30% propolis ethanol extract (PEE) and sodium monensin on the intake of dry matter, nutrient digestibility and ruminal fermentation and hematological parameters in sheep. Six adult castrated male sheep fistulated in the rumen were assigned to a 6 x 6 Latin square design. The addition of PEE or sodium monensin to the diet did not change the digestibility coefficients of dry matter (79.4%), crude protein (77.0%), neutral detergent fiber (76.1%), acid detergent fiber (69.7%), hemicellulose (80.9%), and organic matter (79.4%). Sheep fed diets with inclusion of sodium monensin showed 11.3% reduction in dry matter intake (1.76 kg day-1) compared to those which received PEE (2.00 kg day-1). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutrition; Ionophores; Bacteria; Rumen; Sheep blood.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/25725

Antibacterial activity of different types of snake venom from the Viperidae family against Staphylococcus aureus
Provedor de dados: 3
Autores: Canhas, Isabela Nascimento; Heneine, Luiz Guilherme Dias; Fraga, Thaís; Assis, Débora Cristina Sampaio de; Borges, Márcia Helena; Chartone-Souza, Edmar; Nascimento, Andréa Maria Amaral.
Toxins and venoms produced by living organisms have exhibited a variety of biological activities against microorganisms. In this study, we tested seven snake venoms from the family Viperidae for antibacterial activity and the activities of reversal of antibiotic resistance and inhibition of biofilm formation against 22 clinical isolates of Staphylococcus aureus. Bothrops moojeni venom exhibited anti staphylococcal activity with the lowest mean value of minimum inhibitory concentration (MIC). Moreover, reversal of antibiotic resistance was observed for combinations of B. moojeni venom (½ x MIC) and norfloxacin or ampicillin (both ½ x MIC) for 86.4% and 50% of the isolates, respectively. B. moojeni venom alone at ½ MIC inhibited 90% of biofilm formation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops moojeni; Bacteria; Antibiotic-resistance; NorA efflux pump; Biofilm.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33826

Antimicrobial and enzymatic activity of anemophilous fungi of a public university in Brazil
Provedor de dados: 42
Autores: SOBRAL,LAUREANA V.; MELO,KELLY N.; SOUZA,CLECIANA M.; SILVA,SÍLVIO F.; SILVA,GILVANIA L.R.; SILVA,ANDRESSA L.F.; WANDERLEY,KATHARINE A.A.; OLIVEIRA,IDJANE S.; CRUZ,ROBERTA.
ABSTRACT To the fungal microbiota the UFPE and biotechnological potential enzymatic and antimicrobial production. Air conditioned environments were sampled using a passive sedimentation technique, the air I ratio and the presence of aflatoxigenic strains evaluated for ANVISA. Icelles were to determine the enzymatic activity of lipase, amylase and protease metabolic liquids to determine antimicrobial activity. Diversity was observed in all CAV environments, CFU/m3 ranged from 14 to 290 and I/E ratio from 0.1 to 1.5. The of the fungal genera were: Aspergillus (50%), Penicillium (21%), Talaromyces (14%), Curvularia and Paecilomyces (7% each). Aspergillus sydowii (Bainier &amp; Sartory) Thom &amp; Church presented enzymatic activity and the Talaromyces...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air quality; Bacteria; Enzymes; Filamentous fungi; Refrigerated environments.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502327

Temporal variation of bacterial population and response to physical and chemical parameters along a petrochemical industry wastewater treatment plant
Provedor de dados: 42
Autores: ANTUNES,THEMIS C.; BALLARINI,ANA E.; SAND,SUELI VAN DER.
Abstract: The petrochemical industry has played a considerable role in generation and release of waste in the environment. Activated sludge and facultative lagoons are commonly used for domestic and industrial wastewater treatment due to their low-cost and minimal need for operational requirements. Microorganisms present in wastewater treatment plant (WWTP) are responsible for most nutrient removal. In this study, microbiological and physicochemical parameters were used to estimate changes in bacterial community in a petrochemical industrial WWTP. The activated sludge was the place with higher heterotrophic bacterial quantification. Denitrifying bacteria was reduced at least 5.3 times throughout all collections samples. We observe a decrease in the total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Diversity; Wastewater treatment; Facultative lagoon; Activated sludge.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300616

A structural perspective on the mechanisms of quorum sensing activation in bacteria
Provedor de dados: 42
Autores: LIXA,CAROLINA; MUJO,AMANDA; ANOBOM,CRISTIANE D.; PINHEIRO,ANDERSON S.
Bacteria are able to synchronize the population behavior in order to regulate gene expression through a cell-to-cell communication mechanism called quorum sensing. This phenomenon involves the production, detection and the response to extracellular signaling molecules named autoinducers, which directly or indirectly regulate gene expression in a cell density-dependent manner. Quorum sensing may control a wide range of biological processes in bacteria, such as bioluminescence, virulence factor production, biofilm formation and antibiotic resistance. The autoinducers are recognized by specific receptors that can either be membrane-bound histidine kinase receptors, which work by activating cognate cytoplasmic response regulators, or cytoplasmic receptors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoinducer; Bacteria; Quorum sensing; Receptor; Structure.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502189

Secondary metabolites from marine microorganisms
Provedor de dados: 42
Autores: KELECOM,ALPHONSE.
After 40 years of intensive research, chemistry of marine natural products has become a mature field. Since 1995, there are signals of decreased interest in the search of new metabolites from traditional sources such as macroalgae and octocorals, and the number of annual reports on marine sponges stabilized. On the contrary, metabolites from microorganisms is a rapidly growing field, due, at least in part, to the suspicion that a number of metabolites obtained from algae and invertebrates may be produced by associated microorganisms. Studies are concerned with bacteria and fungi, isolated from seawater, sediments, algae, fish and mainly from marine invertebrates such as sponges, mollusks, tunicates, coelenterates and crustaceans. Although it is still to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Fungi; Alkaloids; Despsipeptides; Terpenoids; Acetogenins; Biological activities.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000100012

Copper II - polar amino acid complexes: toxicity to bacteria and larvae of Aedes aegypti
Provedor de dados: 42
Autores: RODRIGUES,THIAGO A.D.; ARRUDA,EDUARDO J. DE; FERNANDES,MAGDA F.; CARVALHO,CLAUDIO T. DE; LIMA,ALESSANDRA R.; CABRINI,ISAÍAS.
ABSTRACT Control strategies using insecticides are sometimes ineffective due to the resistance of the insect vectors.In this scenario new products must be proposed for the control of insect vectors.The complexes L-aspartate Cu (II) and L-glutamate-Cu (II) complexes were synthesized and characterized by elemental analysis, visible ultraviolet, infrared spectroscopy and potentiometric titration. The toxicity of these complexes was analyzed in Aedes aegypti (Diptera: Culicidae) larvae and Gram-negative and Gram-positive bacteria. The interaction between the ligands and the amino acid balance and the distribution of the species as a function of pH were discussed. The lethal concentration median (LC50) for Ae. aegypti larvae were: L-glutamic acid-Cu (II) -...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurotransmitter; Bacteria; Population control of vectors; Metal-insecticide; Mosquito.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502273

Production of lipolytic enzymes by bacteria isolated from biological effluent treatment systems
Provedor de dados: 42
Autores: FURINI,GRACIANE; BERGER,JUSSARA S.; CAMPOS,JOSÉ A.M.; SAND,SUELI T. VAN DER; GERMANI,JOSÉ C.
Abstract This work aimed to evaluate the production of lipolytic complexes, produced by microorganisms isolated from a biological treatment system of effluents from a hotel. To select the best lipolytic microorganism for use in biotechnological processes, we tested 45 bacterial isolates recovered from the raw effluent of the hotel’s restaurant waste tank. Lipase production was assayed in culture medium supplemented with olive oil and rhodamine B, incubated at 25 °C and 30 °C for 24 h - 48 h. Results showed 22 isolates lipase producers. All isolates were inoculated on medium without yeast extract to select the ones with highest enzyme yields. Out of these, nine isolates showed high lipase activity. The strain with the larger halo was assayed in submerged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipolytic enzymes; Bacteria; Submerged culture; Wastewater.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602955

Seleção de matéria prima para a elaboração de meio de cultura para produção de Bacillus thuringiensis var. kurstaki Berliner
Provedor de dados: 44
Autores: Alves,Luis F. A.; Alves,Sérgio B.; Capalbo,Deise M. F.
The spore production "in vitro" of 43 combinations of agroindustrial by-products (cheese whey, silkworm flour, soybean protein and foder yeast) was evaluated. All media tested showed to be suitable for Bacillus thuringiensis var. kurstaki Berliner production and the concentration of spores obtained in these media varied from 10(6) to 10(9) spores/ml.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Entomopathogen; Bacteria; Culture techniques.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000200022

Quantification of ruminal microbiota and production of methane and carbonic dioxide from diets with inclusion of glycerin
Provedor de dados: 45
Autores: Santos,V.C.; Ezequiel,J.M.B.; Homem Junior,A.C.; Pinheiro,R.S.B.
The aim of this study was to quantify the liquid-associated bacteria and protozoa of the rumen and to evaluate the potential of in vitroproduction of gases CH4 and CO2 of diets with inclusion of glycerin for sheep. Two diets were formulated with a roughage:concentrate ratio of 20:80, according to the presence or absence of glycerin: G0 - control, without glycerin; and G10 - 10% glycerin as part of the concentrate. To quantify the liquid-associated bacteria and protozoa from the rumen and production of CH4 and CO2 gases, a completely randomized design was used. The diets had no effect (P&gt;0.05) on production and composition of liquid-associated protozoa and bacteria from the rumen of lambs. Likewise, a (P&gt;0.05) diet effect was not observed on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Bacteria; Gases; Nitrogen; Protozoa.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000100205

Gamma radiation treatment applied to microbial decontamination of products derived from eggs collected from the retail market in São Paulo
Provedor de dados: 45
Autores: Aquino,S.; Lui,C.C.; Corrêa,B.
ABSTRACT The quality of products derived from eggs depends on the quality of the raw material source and the industrial production. The contamination by fungi and bacteria can occur after exposure of the product in the retail market. The objective of this study was to evaluate the microbiological quality of 60 samples of derivated egg products and evaluate the effects of gamma radiation of cobalt-60, using the dose of 5 and 10 kGy, for decontamination of products collected in the retail market in the São Paulo city. The bacterial count was performed by the most probable number (MPN) and the fungal counts by serial dilution after plating surface. It was observed a decrease in the humidity and water activity (Aw) values of irradiated whole egg powder and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Sterilization; Fungi; Irradiation; Powdered egg.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601683

Resistance to antibiotics in Gram-negative bacteria isolated from broiler carcasses
Provedor de dados: 45
Autores: Moreira,M.A.S.; Moraes,C.A.
One hundred and ninety-seven isolates of Gram-negative bacteria, comprising 10 genera, were isolated from poultry carcasses at a processing plant in order to investigate resistance to low levels of antibiotics. The samples were taken just after evisceration and before inspection. Most of the isolates were of Samonella and Escherichia. Other genera present were Enterobacter, Serratia, Klebsiella, Kluyvera, Erwinia, Citrobacter, Pseudomonas and Aeromonas. Distinct profiles of antibiotic resistance were detected. Resistance to more than two antibiotics predominated and spanned several classes of antibiotics. Salmonellae and escherichiae were mainly resistant to the aminoglycosides, followed by tetracycline, nitrofuran, sulpha, macrolide, chloramphenicol,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Poultry; Antibiotic; Multi-resistance; Bacteria; Growth promoter.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000100001

In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production
Provedor de dados: 45
Autores: Leopoldino,W.M.; Lana,R.P.; Borges,A.C.; Mantovani,H.C.; Teixeira,R.M.A.; Oliveira,J.S.; Jaremtchuk,A.R.; Eifert,E.C.; Martins,R.G.R.
Ruminal fluid from steers fed on pasture was incubated with artificial media at pH 5.5 and 7.0 in two experiments. In the first, the effect of monensin level on resistance of ruminal bacteria to potassium depletion was evaluated; in the second, effects of the ionophores monensin and lasalocid on ammonia and protein production were quantified. In experiment 1, culture media affected potassium level. The monensin concentration needed to cause half maximal potassium depletion was 2.77µM at pH 5.5 but was 0.056µM at pH 7.0, showing that bacteria incubated at pH 5.5 were more tolerant to monensin than those incubated at pH 7.0. Both ionophores as well as increased acidity caused decreased ammonia production. Both ionophores inhibited ammonia production by 56%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Lasalocid; Monensin; PH; Rumen.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600012

Identification and antimicrobial susceptibility of bacteria isolated from corneal ulcers of dogs
Provedor de dados: 45
Autores: Prado,M.R.; Brito,E.H.S.; Girão,M.D.; Sidrim,J.J.C.; Rocha,M.F.G.
A total of 22 clinical specimens were obtained from 19 dogs with corneal ulcer (16 unilateral and three bilateral) for isolation and antimicrobial susceptibility evaluation of the isolated bacteria. Bacterial growth was observed in 100% of the samples (n=22). Staphylococcus intermedius was the predominant species (35.5%), followed by Corynebacterium xerosis (19.3%). Gentamicin, ciprofloxacin, chloramphenicol and tobramycin had a high efficacy against all of the isolated bacteria. The results evidenced that 80.7% of the isolates were Gram positive cocci and Gram positive bacilli, and that those microorganisms were sensitive to gentamicin, ciprofloxacin, chloramphenicol and tobramycin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Corneal ulcer; Bacteria.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600008

Sponges in Peru and their potential as a source of antibacterial compounds
Provedor de dados: 50
Autores: Cóndor-Luján,Báslavi; Francia-Quiroz,Juan C.
ABSTRACT In the last decades, sponges have emerged as the largest source of active compounds of animal origin for pharmaceutical purposes. In Peru information on these animals is incipient and scattered, which limits taking the right measures for their adequate management, exploitation, and conservation. The aim of this study is to provide an update on the scope and perspectives of sponge research in Peru, with an emphasis on species richness and their antibacterial potential. Likewise, research initiatives carried out in other South American Pacific countries are reviewed. Research efforts in Peru have allowed the description of 46 species; however, the sponge richness is underestimated. On the other hand, in other latitudes, active principles with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial activity; Bacteria; Biotechnology; Marine biodiversity; Porifera.
Ano: 2022
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612022000200107

EFECTO DE LAS CONDICIONES DE TRANSPORTE, RECEPCIÓN, ACLIMATACIÓN Y SIEMBRA DE NAUPLIOS DE LITOPENAEUS VANNAMEI (BOONE, 1931) SOBRE LA SOBREVIVENCIA EN LARVICULTURA.
Provedor de dados: 50
Autores: Suárez,Jorge A.; García,Alberto; Newmark,Federico; Bador,Regis.
Con el objeto de determinar los factores iniciales que pueden afectar la sobrevivencia de postlarvas de camarón durante el proceso de larvicultura, se muestrearon tres laboratorios ubicados en la costa Atlántica de Colombia, cubriendo 71 lotes de nauplios en un período de 10 meses durante 1996. Para cada lote de nauplios se hizo seguimiento de variables cualitativas (origen, modo de transporte, presencia de bacterias en el agua de transporte) y variables cuantitativas (tiempo y densidad de transporte, características de la calidad del agua, proporciones de cada estadío y deformidad de los nauplios). Las variables calidad del agua y densidad (nauplios/l) se evaluaron durante los procesos de empaque, transporte, recepción, aclimatación y siembra de los...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nauplio; Calidad; Bacteria; Transporte.
Ano: 2001
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612001000100001

PROBIÓTICOS COMO HERRAMIENTA BIOTECNOLÓGICA EN EL CULTIVO DE CAMARÓN: RESEÑA
Provedor de dados: 50
Autores: Villamil Díaz,Luisa; Martínez-Silva,María Angélica.
Las bacterias probióticas se definen como microorganismos vivos que administrados como suplemento en la dieta pueden causar modificaciones en la microbiota asociada al tracto gastrointestinal del hospedador y generar efectos benéficos como el incremento en la conversión alimentaria, en la resistencia a enfermedades y de la calidad del agua. Durante la última década, su aplicación en el cultivo de camarón se ha hecho frecuente ya que han surgido varios productos comerciales ideados para este fin. Al mismo tiempo, aunque hay publicados varios artículos científicos en el tema, es evidente que hace falta orientar los estudios para entender los mecanismos de acción de los probióticos, así como para establecer los protocolos de aplicación, teniendo en cuenta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Cultivo de camarón; Probióticos; Bacillus; Lactobacillus.
Ano: 2009
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612009000200009

Microbial Diversity: Relevance and Relationship Between Environmental Conservation And Human Health
Provedor de dados: 52
Autores: Panizzon,Jeremias Pakulski; Pilz Júnior,Harry Luiz; Knaak,Neiva; Ramos,Renata Cristina; Ziegler,Denize Righetto; Fiuza,Lidia Mariana.
This work presents bibliographic data on the role and function of microbial diversity. The increasing use of probiotics and prebiotics foods has led to the studies on their actual functions in the human body. It is known that in the environment, microorganisms are extremely important in recycling of nutrients, balance of trophic chains, vital physiological activities in the plants and animals, as well as the conservation of natural habitats. In human food, these microscopic organisms contribute from flavoring products to the synthesis of antimicrobial substances and vitamins essential to living beings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Nutraceutical; Collective health; Review.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100137

Effect of buctril super (Bromoxynil) herbicide on soil microbial biomass and bacterial population
Provedor de dados: 52
Autores: Abbas,Zafar; Akmal,Muhammad; Khan,Khalid Saifullah; Fayyaz-ul-Hassan,.
The present study aimed to evaluate the effect of bromoxynil herbicide on soil microorganisms, with the hypothesis that this herbicide caused suppression in microbial activity and biomass by exerting toxic effect on them. Nine sites of Punjab province (Pakistan) those had been exposed to bromoxynil herbicide for about last ten years designated as soil 'A' were surveyed in 2011 and samples were collected and analyzed for Microbial Biomass Carbon (MBC), Biomass Nitrogen (MBN), Biomass Phosphorus (MBP) and bacterial population. Simultaneously, soil samples from the same areas those were not exposed to herbicide designated as soil 'B' were taken. At all the sites MBC, MBN and MBP ranged from 131 to 457, 1.22 to 13.1 and 0.59 to 3.70 µg g-1 in the contaminated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomass; Buctril super; Bacteria; Effect; Population.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100002

Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts
Provedor de dados: 52
Autores: Meneghin,Maria Cristina; Reis,Vanda Renata; Ceccato-Antonini,Sandra Regina.
The aim of this work was to study the in vitro antibacterial activity possessed by killer yeast strains against bacteria contaminating alcoholic fermentation (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides), in cell X cell and cell X crude toxin preparations. The bacteria were not inhibited by any S. cerevisiae killer strains (5 out of 11). The inhibition caused by two crude toxin preparations (Trichosporon figueirae and Candida sp) against L. plantarum was surprisingly high but not in the same extent for B. subtilis, especially with three killer strains (Candida glabrata, Pichia anomala and Candida sp). L. mesenteroides and L. fermentum strains were neither inhibited in cell X cell nor crude toxin X cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Killer yeasts; Alcohol; Fermentation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500006

Microbiological analysis of critical points in the chicken industry
Provedor de dados: 52
Autores: Cansian,Rogério Luis; Floriani,Salete Teresa Radeski; Valduga,Eunice.
This work is focused on identifying microbial contamination in the scalding asepsis and cooling processes as well as in fresh sausages obtained. Salmonella was identified in two scald water samples but was absent in the water from chiller and in the final product, which might be explained in terms of chlorine addition and temperature reduction. The analysis revealed that MPN of Escherichia coli was in the range of &lt;1-11/mL in the scald water and &lt;1-64/mL in the chiller. Aeromonas sp. count showed 5 to 3.5x10¹ CFU/mL in the scald water and 9 to 3.7x10² CFU/mL in the chiller, probably due to the psycrophile characteristics of Aeromonas and evisceration process. The analysis of fresh sausages chicken revealed a sharp increase in the Aeromonas count...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas; Bacteria; Chicken; Microbiology; Food quality control.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300011

Studies on fungal and bacterial population of air-conditioned environments
Provedor de dados: 52
Autores: Ross,Claudia; Menezes,Jose Roberto de; Svidzinski,Terezinha Inez Estivalet; Albino,Ulisses; Andrade,Galdino.
In tropical countries such as Brazil, there is not enough information about microbial contaminants in indoor environments with air conditioning systems. Microbial monitoring of such environments is important for the quality of human life. The aim of this work was to assess the fungal genera and bacterial morphotypes occurring in such environments. Air samples were taken indoors and outdoors from a public auditorium, a hospital, a company and a shopping center during the 2001 winter by using a six-stage impactor Millipore M air T® . Twenty-one fungal genera were identified. Bacterial morphological groups found were Gram positive and negative rods and Gram positive coccus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sick building syndrome; Fungi; Bacteria; Air conditioner; Indoor air quality; Air ecology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500020

Measurement of volatilized mercury by a mini-system: a simple, reliable and reproducible technique
Provedor de dados: 52
Autores: Cursino,Luciana; Mattos,Silvânia V. M.; Silva,Nilton O.; Chartone-Souza,Edmar; Nascimento,Andréa M. A.
A simple and easy new technique for volatilized mercury determination in biological systems was developed. This technique is fast and sensitive and can overcome the problems that arise due to the extremely low readings during the measurements and reproducibility in biological material (bacteria). It measures directly the volatilized metallic mercury of bacteria by means of a chemical adsorbent in a coupled mini-system, as a modified technique for mercury in air analysis. It is potentially of interest to the bioremediation and bacterial mercury resistance communities
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Biological system; Measurement; Mercury; Volatilization.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400028

The abundance of some pathogenic bacteria in mangrove habitats of Paraiba do Norte estuary and crabmeat contamination of mangrove crab Ucides cordatus
Provedor de dados: 52
Autores: Grisi,Teresa Cristina Soares de Lima; Gorlach-Lira,Krystyna.
Study on mangrove water and sediment of Paraiba do Norte river (Northeastern Brazil) and crabmeat of Ucides cordatus was carried to investigate the relation between the abundance of bacteria in the mangrove habitat and in the crabmeat. Simultaneous collection of samples of water, sediment and crabs (U. cordatus) was done during the rainy and dry seasons at two areas of mangroves that differed in influence of tide and sewage discharge. The numbers of total heterotrophic bacteria in the mangrove water were between 0.04 and 6 log CFU 100mL-1, and in the sediment between 5 and 6 log CFU g-1. The location closer to waste discharge showed higher incidence of total heterotrophic bacteria, coliforms, Staphylococcus aureus and Salmonella spp. The counts of total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangrove ecosystem; Bacteria; Ucides cordatus; Crabmeat; Contamination.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100028

Ardra profiles of bacteria and archaea in mangrove sediments with different levels of contamination in the estuarine complex of Paranaguá, Brazil
Provedor de dados: 52
Autores: Ribeiro,Catherine Gérikas; Steffens,Maria Berenice Reynaud; Etto,Rafael Mazer; Galvão,Carolina Weigert; Martins,César de Castro; Pedrosa,Fábio de Oliveira; Kolm,Hedda Elisabeth.
The mangrove's sediments from the coastal areas under human activities may contain significant contaminations by hydrocarbons, even when there are no visual evidences of it. The microorganisms are essential to these ecosystems, especially in the control of their chemical environment. Sediment samples were collected in two regions under different environment conditions (pristine and contaminated) of the Paranaguá Estuarine Complex (Paranaguá Bay and Laranjeiras Bay), Brazil. Aliphatic hydrocarbons were determined by the GC-FID to assess the status of contamination of the studied areas. The total DNA was extracted from these samples. The 16S rRNA gene was amplified by the PCR reactions with the pair of primers 21F and 958R for the archaeal domain, and 27F...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diversity; Mangroves; Archaea; Bacteria; 16S rRNA; ARDRA; Aliphatic hydrocarbons.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200013

Spatial Variation of Bacteria in Surface Waters of Paranaguá and Antonina Bays, Paraná, Brazil
Provedor de dados: 52
Autores: Kolm,Hedda Elisabeth; Schoenenberger,Maria Fernanda; Piemonte,Mariana da Rocha; Souza,Patrícia S. de A.; Scühli,Guilherme Schnell e; Mucciatto,Mariele B.; Mazzuco,Roseli.
Spatial variability in the concentration of total bacteria, saprophytes and coliforms abundance was investigated in surface waters of Paranaguá and Antonina Bays. Six points along a profile from the entrance of Paranaguá Bay to the innermost part of Antonina Bay, were sampled on February 26, 1997. Temperature, salinity, dissolved oxygen, pH, particulate organic carbon, water transparency and seston were also measured. Determination of total bacterial abundance and biomass were made with the epifluorescent method. Saprophytic bacteria counts were conducted with ZoBell 2216E culture medium with fresh and 32‰ water and the coliform MPN was enumerated with the chromogenic substrate method. Principal component analysis of biotic and abiotic data showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Hydrographic variables; Estuary; Paranaguá and Antonina Bays.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100005

Reducing COD level on oily effluent by utilizing biosurfactant-producing bacteria
Provedor de dados: 52
Autores: Jacobucci,Daniela Franco Carvalho; Oriani,Maria Raquel de Godoy; Durrant,Lucia Regina.
Two bacteria isolated from crude oil contaminated soil, Pantoea agglomerans and Planococcus citreus, produced biosurfactants utilizing 1.5% of kerosene and olive oil as the sole carbon sources, respectively. The bacteria and the biosurfactants produced were introduced to oily effluent, arising from margarine and soap industry. Emulsification activities were determined by increases in the absorbance of the oil-in-water emulsions at 610 nm, whereas the water-in-oil emulsions were expressed as the height (cm) of the emulsion layers formed. The 72 h incubation experiment resulted in a COD (Chemical Oxygen Demand) reduction of 76% with Planococcus citreus strain and 70% with Pantoea agglomerans.The COD reduction with bacterial biosurfactants was over 50% in 24...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosurfactants; Bacteria; COD; Oily effluent; Bioremediation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400029

Direct RAPD evaluation of bacteria without conventional DNA extraction
Provedor de dados: 52
Autores: Araújo,Welington Luiz; Angellis,Derlene Attili de; Azevedo,João Lúcio.
The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were centrifuged and the resulting preparation was suspended in TE buffer. After boiling, the cell suspension was diluted and 2.0 µl were used in reactions of 15 µl. The results showed no significant differences among the RAPD profile of the PCR reactions derived from the boiling and phenol extraction methods, suggesting the utilization of this method for genetic population analysis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Boiling method; DNA extraction; RAPD.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006

Functional Genes of Microorganisms, Comprehending the Dynamics of Agricultural Ecosystems
Provedor de dados: 52
Autores: Reali,Catiusca; Pilz Júnior,Harry Luiz; Meile,Jean Christophe; Galindo,Sabine Shorr; Fiuza,Lidia Mariana.
ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to maintain the sustainability of a crop. Molecular tools to study microbial communities are possible through many methods such as RISA, DGGE, TGGE, clone libraries, T-RFLP, RAPD, SSCP and more recently NGS (Next-Generation Sequencing). DGGE is widely employed to characterize the diversity and the community dynamics of microorganisms in the environment, making possible to find out specific groups through functional genes, allowing access to data that cannot be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agroecosystems; Functional genes; Bacteria; DGGE; Metagenomics; Microbial ecology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100201

Seleção de bacillus spp. para produção de esterases e melhoramento de bacillus cereus (c124)
Provedor de dados: 52
Autores: Mendonça,Analucia Longman; Mariano,Rosa de Lima Ramos; Araújo,Janete Magali de; Cavalcante,Uided Maaze T.
Forty-four Bacillus spp. strains obtained from sugar cane derivates and residues, six of them isolated in this work, were tested using Tween 80 as substrate (agar-Tween 80 medium), in order to determine their esterase activity through the enzymatic index averages. After statistic analysis, B. cereus (C124) strain, which presented better results, was submitted to genetic improvement by treatment with ultraviolet light (UV). The survival curve pointed out 28" as the time necessary to obtain 30% of survivors. Fifty survivors and the wild strain C124 were compared in relation to their esterase activity as mentioned previously. The wild strain and the mutant C124UV35, which showed enzymatic index average higher than C124, were characterized in polyacrilamide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Genetic improvement; Bacillus spp.; B. cereus; Esterase.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100011

New aspects on atrazine biodegradation
Provedor de dados: 52
Autores: Sene,Luciane; Converti,Attilio; Secchi,Geslaine Aparecida Ribeiro; Simão,Rita de Cássia Garcia.
The world practice of using agrochemicals for long periods, in an indiscriminated and abusive way, has been a concern of the authorities involved in public health and sustainability of the natural resources, as a consequence of environmental contamination. Agrochemicals refer to a broad range of insecticides, fungicides and herbicides, and among them stands out atrazine, a herbicide intensively used in sugarcane, corn and sorghum cultures, among others. Researches have demonstrated that atrazine has toxic effects in algae, aquatic plants, aquatic insects, fishes and mammals. Due to the toxicity and persistence of atrazine in the environment, the search of microbial strains capable of degrading it is fundamental to the development of bioremediation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atrazine; Biodegradation; Bacteria; Fungi; Microbial consortium.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200030

Indoor Air Quality in the Hospital: The Influence of Heating, Ventilating and Conditioning Systems
Provedor de dados: 52
Autores: Božić,Jelena; Ilić,Predrag; ilić,Svetlana.
Abstract Clean, fresh air is the most important requirement for good indoor air quality (IAQ) in all buildings, but it is especially important with regard to the environments within hospitals and other healthcare facilities. The literature indicates that buildings with heating, ventilating and air conditioning (HVAC) systems may have an increased risk of sick building syndrome (SBS) and building-related illness (BRI) if they are not well maintained. Microorganisms are brought into hospitals by people, air currents, water, construction materials and equipment. The main objective of this study was to assess the degree of fungal and bacterial contamination in the University Clinical Centre in Banja Luka, which is directly connected to the HVAC system....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indoor air quality; Hospital; HVAC system; Bacteria; Fungi.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100607

Antifouling effect of bioactive compounds from marine sponge Acanthella elongata and different species of bacterial film on larval attachment of Balanus amphitrite (cirripedia, crustacea)
Provedor de dados: 52
Autores: Ganapiriya,Viswambaran; Maharajan,Athisuyambulingam; Kumarasamy,Pothiappan.
The antifouling activity of bioactive compounds from marine sponge Acanthella elongata (Dendy) and five species of bacterial biofilm were studied. Larvae of Balanus amphitrite (Cyprids and nauplii) were used to monitor the settlement inhibition and the extent to which inhibition was due to toxicity. The crude extract and partially purified fractions of A.elongata showed significant inhibition over the settlement individually, and with the interaction of bacterial species. No bacterial film stimulated the barnacle settlement. The high but variable levels of antifouling activity in combination with less amount of toxicity showed the potential of these metabolites in environmentally-friendly antifouling preparations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofouling; Bacterial film; Bacteria; Barnacles; Cyprids; B. amphitrite.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300010

Decontamination of Pig Carcasses Using Water Pressure and Lactic Acid
Provedor de dados: 52
Autores: Brustolin,Jean Carlos; Dal Pisol,Andreia; Steffens,Juliana; Toniazzo,Geciane; Valduga,Eunice; Di Luccio,Marco; Cansian,Rogério Luis.
The objective of this work was to evaluate the effect of different water pressures and concentrations of lactic acid on microbial counts (mesophilic bacteria, enterobacteria and Salmonella) on pig carcasses without contamination and contaminated carcasses, before and after the last shower and before being cooled. The tests were carried out using 4, 3 and 2 bar water pressure, and 2, 1 and 0% lactic acid concentration. In general, both the pressure in the shower and lactic acid had a positive effect by reducing the microbial count. The interaction between the pressure and lactic acid caused the largest reduction in carcasses surface count for mesophiles. With regard to enterobacteria on contaminated carcasses, the most important variable was the lactic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig carcasses; Decontamination; Water pressure; Lactic acid; Bacteria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600954

Hospital gowns as a vehicle for bacterial dissemination in an intensive care unit
Provedor de dados: 55
Autores: Pilonetto,Marcelo; Rosa,Edvaldo Antonio Ribeiro; Brofman,Paulo Roberto Slud; Baggio,Daniela; Calvário,Francine; Schelp,Cristiane; Nascimento,Aguinaldo; Messias-Reason,Iara.
The microbiota from the uniforms of 31 professionals from the general intensive care unit was analyzed. The samples were collected in duplicate at the beginning and at the end of the work period. Total viable counts of microorganisms were determined; there was a significant increase in the counts at the end of the period, when compared with those obtained at the beginning. No significant difference was observed between the first and second counts obtained from the cuffs. However, differences were observed for the samples from the abdominal region. Among the isolated pathogens 11/18 were Staphylococcus aureus, 2/18 were Acinetobacter baumannii, 2/18 were Klebsiela pneumoniae and 1/18 were Serratia rubidae. Some of these isolates were multi-resistant to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gown; Bacteria; Hospital infection; Cross contamination.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000300003

Morbimortality study of infection in patients undergoing different types of dialysis in a renal replacement therapy center
Provedor de dados: 55
Autores: Curty,Natália Fabiane Ridão; Martins,Lucilene Fagundes da Silva; Ito,Carmen Antônia Sanches; Schafranski,Marcelo; Brites,Dorelayne Aparecida; Busato,César Roberto.
INTRODUCTION: Renal replacement therapy is the treatment of end-stage chronic kidney disease and can be performed through dialysis catheters, arteriovenous fistulas/grafts, and peritoneal dialysis. Patients are usually immunocompromised and exposed to invasive procedures, leading to high rates of infection and increased mortality.OBJECTIVES: To compare the prevalence of infection and related deaths, as well as the sensitivity profile of the putative bacteria in patients treated with peritoneal dialysis, arteriovenous fistula hemodialysis and catheter hemodialysis.METHODS: This is case-control study. Six hundred forty-four patients undergoing renal replacement therapy were selected. Patients were divided into three groups according to the modality of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hospital infection; Morbimortality; Renal replacement therapy center; Hemodialysis; Bacteria; Sensitivity profiles.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300281

Evaluation of different detection methods of biofilm formation in the clinical isolates
Provedor de dados: 55
Autores: Hassan,Afreenish; Usman,Javaid; Kaleem,Fatima; Omair,Maria; Khalid,Ali; Iqbal,Muhammad.
BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. OBJECTIVE: This study was conducted to compare three methods for the detection of biofilms. METHOD: The study was carried out at the Department of Microbiology, Army Medical College, National University of Sciences and Technology, Pakistan, from January 2010 to June 2010. A total of 110 clinical isolates were subjected to biofilm detection methods. Isolates were identified by standard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Bacteria; Anti-bacterial agents.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400002

Catheter-related infections in a northwestern São Paulo reference unit for burned patients care
Provedor de dados: 55
Autores: Campos Júnior,Cláudio Penido; Sanches,Patrícia; Tedokon,Elizabete Aparecida; Souza,Ana Carolina Remondi; Machado,Ricardo Luiz Dantas; Rossit,Andréa Regina Baptista.
Despite improvements in care and rehabilitation of burned patients, infections still remain the main complication and death cause. Catheter-related infections are among the four most common infections and are associated with skin damage and insertion site colonization. There are few studies evaluating this kind of infection worldwide in this special group of patients. Padre Albino Hospital Burn Care Unit (PAHBCU) is the only reference center in the Northwestern São Paulo for treatment of burned patients. This paper presents the results of a retrospective study aiming at describing the epidemiological and clinical features of catheter-related infections at PAHBCU.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catheter; Infections; Burns; Bacteria.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200009

Identifying and controlling a multiresistant pseudomonas aeruginosa outbreak in a latin-american cancer centre and its associated risk factors
Provedor de dados: 55
Autores: Cortes,Jorge Alberto; Cuervo,Sonia Isabel; Urdaneta,Ana María; Potdevin,Guillermo; Arroyo,Patricia; Bermúdez,Diana; Correa,Adriana; Villegas,María Virginia.
Pseudomonas aeruginosa is an important and frightening microorganism for patients suffering from cancer. Multiresistant P. aeruginosa (MRPA) may appear as a consequence of exposure to multiple antibiotics or from a breakdown in infection control practices. This article reports an MRPA outbreak in a cancer treatment centre and the consequent case control study. Mechanical ventilation was identified as being the main risk factor for developing MRPA colonisation or infection; molecular analysis confirmed the outbreak. A multifaceted strategy was adopted, involving reinforcing hand-washing practices, contact isolation, antibiotic restriction and suction devices for mechanically-ventilated patients. MRPA was controlled and the outbreak ended. Such strategy may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Epidemiology; Multiple drug resistance; Bacteria; Risk factor.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200005

Bacterial contamination in milk kitchens in pediatric hospitals in Salvador, Brazil
Provedor de dados: 55
Autores: Cairo,Romilda Castro; Silva,Luciana Rodrigues; Andrade,Carol Ferreira de; Barberino,Maria Goreth de Andrade; Bandeira,Antônio Carlos; Santos,Kleber Pimentel; Diniz-Santos,Daniel R.
Milk may represent an important source of infectious agents to hospitalized pediatric patients. To describe the bacterial microflora isolated from the hands, stools, pharynx of all workers at milk kitchens in pediatric hospitals in the city of Salvador, Brazil, as well as in the formulas prepared by them, we carried out this cross-sectional study with all 91 workers from the 20 milk kitchens of all the public and private hospitals in Salvador, Brazil. Hand and pharynx swabs and stool samples were collected from all workers, as well as samples of the milk and formulas delivered by the kitchens. All samples were cultured for the detection of pathogenic and non-pathogenic bacteria. Pathogenic bacteria were isolated from 20 (22.0%) and 8 (8.8%) cultures of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Microflora; Hands; Faeces; Pharynx; Milk; Contamination; Hospital; Children.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300011

Trends in bacterial resistance in a tertiary university hospital over one decade
Provedor de dados: 55
Autores: Góngora Rubio,Fernando; Oliveira,Viviane Decicera Colombo; Rangel,Regina Mara Custódio; Nogueira,Mara Corrêa Lelles; Almeida,Margarete Teresa Gottardo.
The objective of this study was to investigate bacterial resistance trends, infection sites and the relationship between resistance and admittance to the intensive care unit (ICU). A total of 53,316 bacteria identified between 1999 and 2008 were evaluated. Multidrug resistance was characterized when gram-negative bacilli (GNB) presented resistance to two or more classes of antibiotics. Gram-positive cocci (CPC) were assessed for resistance to penicillin, oxacillin and vancomycin. GNB were the most common (66.1%) isolate. There was a 3.7-fold overall increase in multidrug resistant GNB over the study period; Acinetobacter baumanii and Staphylococcus aureus were the most prevalent. Highest increases were recorded for Kleb siella pneumoniae (14.6-fold) and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Multiresistance; Bacteria; Trends.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400014

IL-6 and IL-8 in cerebrospinal fluid from patients with aseptic meningitis and bacterial meningitis: their potential role as a marker for differential diagnosis
Provedor de dados: 55
Autores: Pinto Junior,Vitor Laerte Laerte; Rebelo,Maria Cristina; Gomes,Rachel Novaes; Assis,Edson Fernandes de; Castro-Faria-Neto,Hugo C; Bóia,Marcio Neves.
Cytokines are molecules that act as mediators of immune response; cerebral spinal fluid (CSF) IL-6 is found in all meningeal inflammatory diseases, but IL-8 is associated with acute bacterial meningitis (ABM). A case control study was done to ascertain the discriminatory power of these cytokines in differentiating ABM from aseptic meningitis (AM); IL-6 and IL-8 CSF concentrations were tested through ELISA in samples collected from patients who underwent investigation for meningitis. Sixty patients, 18 with AM, nine with bacteriologic confirmed ABM and 33 controls, assisted in 2005 (MA and controls) and 2007 (ABM) were included. Differently from controls, IL-6 concentrations were increased both in MA and ABM patients (p &lt; 0.05). CSF IL-8 levels were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CSF; Inflammation meningitis; Aseptic meningitis; Bacteria; IL-6; IL-8.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200011

Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil
Provedor de dados: 56
Autores: Pisa,G.; Magnani,G.S.; Weber,H.; Souza,E.M.; Faoro,H.; Monteiro,R.A.; Daros,E.; Baura,V.; Bespalhok,J.P.; Pedrosa,F.O.; Cruz,L.M.
Sugarcane is an important agricultural product of Brazil, with a total production of more than 500 million tons. Knowledge of the bacterial community associated with agricultural crops and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria associated with the plant by culture-dependent approaches. Culture-independent approaches allow a more comprehensive view of entire bacterial communities in the environment. In the present study, we have used this approach to assess the bacterial community in the rhizosphere soil of sugarcane at different times and under different nitrogen fertilization conditions. At the high taxonomic level, few...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 16S rRNA; Biodiversity; Nitrogen fixation; Bacteria; Sugarcane; Soil.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200004

Comparison of serum PCT and CRP levels in patients infected by different pathogenic microorganisms: a systematic review and meta-analysis
Provedor de dados: 56
Autores: Tang,Jun-Hua; Gao,Dong-Ping; Zou,Peng-Fei.
To avoid the abuse and misuse of antibiotics, procalcitonin (PCT) and C-reactive protein (CRP) have been used as new approaches to identify different types of infection. Multiple databases were adopted to search relevant studies, and the articles that satisfied the inclusion criteria were included. Meta-analyses were conducted with Review Manager 5.0, and to estimate the quality of each article, risk of bias was assessed. Eight articles satisfied the inclusion criteria. The concentrations of both PCT and CRP in patients with bacterial infection were higher than those with non-bacterial infection. Both PCT and CRP levels in patients with G− bacterial infection were higher than in those with G+ bacterial infection and fungus infection. In the G+ bacterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary infection; PCT; CRP; Bacteria; Fungus.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700610

Antibacterial activity of plant extracts from Brazil against fish pathogenic bacteria
Provedor de dados: 58
Autores: Castro,S.B.R.; Leal,C.A.G.; Freire,F.R.; Carvalho,D.A.; Oliveira,D.F.; Figueiredo,H.C.P.
The aim of this work was to evaluate the antibacterial activity of Brazilian plants extracts against fish pathogenic bacteria. Forty six methanolic extracts were screened to identify their antibacterial properties against Streptococcus agalactiae, Flavobacterium columnare and Aeromonas hydrophila. Thirty one extracts showed antibacterial activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant extracts; Bacteria; Fish.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400030

Bacterioplancton in different tides of the Perequê tidal creek, Pontal do Sul, Paraná, Brazil
Provedor de dados: 58
Autores: Kolm,Hedda Elisabeth; Andretta,Lucimar.
The influence of tidal phase on bacteria abundance in surface waters was studied in five stations along the Perequê tidal creek (Paraná State, Brazil), at spring and neap low and high tides in July 1999. The stations were located from the mouth (St.1) to the creek's head (St. 5). The following parameters were analyzed: salinity, temperature, pH, dissolved oxygen, total carbon dioxide, seston, particulate organic matter, pluviosity, chlorophyll "a", total heterotrophic bacteria, bacterial biomass, cultivable heterotrophic aerobic halophilic and halophobic bacteria, total coliforms and Escherichia coli. The results showed that the highest values of salinity, pH and dissolved oxygen at stations 1 to 4 were registered during the spring high tide. The amount of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Tide; Tidal creek; Perequê; Paraná State.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200002

Phylogenetic analysis on the soil bacteria distributed in karst forest
Provedor de dados: 58
Autores: Zhou,JunPei; Huang,Ying; Mo,MingHe.
Phylogenetic composition of bacterial community in soil of a karst forest was analyzed by culture-independent molecular approach. The bacterial 16S rRNA gene was amplified directly from soil DNA and cloned to generate a library. After screening the clone library by RFLP, 16S rRNA genes of representative clones were sequenced and the bacterial community was analyzed phylogenetically. The 16S rRNA gene inserts of 190 clones randomly selected were analyzed by RFLP and generated 126 different RFLP types. After sequencing, 126 non-chimeric sequences were obtained, generating 113 phylotypes. Phylogenetic analysis revealed that the bacteria distributed in soil of the karst forest included the members assigning into Proteobacteria, Acidobacteria, Planctomycetes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karst forest; Bacteria; 16S rRNA gene.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400013

Protozoans bacterivory in a subtropical environment during a dry/cold and a rainy/warm season
Provedor de dados: 58
Autores: Hisatugo,Karina F.; Mansano,Adrislaine S.; Seleghim,Mirna H.R.
In aquatic ecosystems, bacteria are controlled by several organisms in the food chain, such as protozoa, that use them as food source. This study aimed to quantify the ingestion and clearance rates of bacteria by ciliates and heterotrophic nanoflagellates (HNF) in a subtropical freshwater reservoir (Monjolinho reservoir -São Carlos -Brazil) during one year period, in order to verify their importance as consumers and controllers of bacteria in two seasons, a dry/cold and a rainy/warm one. For this purpose, in situ bacterivory experiments were carried out bimonthly using fluorescently labeled bacteria with 5-(4,6 diclorotriazin-2yl) aminofluorescein (DTAF). Although ciliates have shown the highest individual ingestion and clearance rates, bacterivory was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bacteria; Grazing; Ciliates; Nanoflagellates; Microbial food web.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100020

Bacterial diversity in soil in response to different plans, phosphate fertilizers and liming
Provedor de dados: 58
Autores: Silva,Paulo da; Nahas,Ely.
The diversity of bacterial isolates from soil in response to different plants (control, Brachiaria ruziziensis and Cajanus cajan), fertilization (control, simple superphosphate and rock phosphate) and liming (with and without lime) was evaluated. Phenotypic and physiological characteristics of the isolates were recorded and organized in a file to identify the bacteria. Among the isolates, 95% were Gram-positive and 5% Gram-negative rods. Soil cultivated with B. ruziziensis favored the nonsporing Gram-positive and Gram-negative rods compared to soils with C. cajan or uncultivated. Number of spore-forming Gram-positive rods were higher in plots with superphosphate than in unfertilized soil or soil fertilized with rock phosphate. In unfertilized plots, larger...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Phosphate; Brachiaria ruziziensis; Cajanus cajan; Liming.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400005

Hematology and productive performance of nile tilapia (Oreochromis niloticus) naturally infected with Flavobacterium columnare
Provedor de dados: 58
Autores: Sebastião,F. A; Nomura,D; Sakabe,R; Pilarski,F.
Columnaris disease is one of the main causes of mortality in tilapia rearing and is responsible for large economic losses worldwide. Hematology is a tool that makes it possible to study organisms' physiological responses to pathogens. It may assist in making diagnoses and prognoses on diseases in fish populations. The hematological variables of nile tilapia were studied in specimens with a clinical diagnosis of columnaris disease and in specimens that were disease-free. The total erythrocyte count, hemoglobin rate, hematocrit percentage, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), organic defense blood cell percentages (leukocytes and thrombocytes) and hepatosomatic and splenosomatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Bacteria; Hematology; Productive performance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100036

Isolation and characterization of Caulobacter mutants impaired in adaptation to stationary phase
Provedor de dados: 58
Autores: Italiani,Valéria C. S.; Marques,Marilis V.
The entry into stationary phase causes a change in the pattern of gene expression of bacteria, when the cells must express a whole set of genes involved mainly with resistance to starvation and to environmental stresses. As an attempt to identify genes important for the survival of Caulobacter crescentus in stationary phase, we have screened a library of 5,000 clones generated by random transposon mutagenesis for mutants that showed reduced viability after prolonged growth. Four clones were selected, which displayed either lower viability or a longer time of recovery from stationary phase. The genes disrupted were identified, and the gene products were found to be mainly involved with amino acid metabolism (glutamate N-acetyltransferase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stationary phase; Stress; Transposon; Bacteria.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100018

Use of Pseudomonas stutzeri and Candida utilis in the improvement of the conditions of Artemia culture and protection against pathogens
Provedor de dados: 58
Autores: Abdelkarim,Mahdhi; Kamel,Chaieb; Fathi,Kammoun; Amina,Bakhrouf.
To evaluate the effect of two bacterial strains isolated from Artemia cysts and yeast (Candida utilis) on the survival, growth and total biomass production of its larvae, challenge tests were performed with Candida utilis, Pseudomonas stutzeri and Pasteurella haemolityca. In addition, a pathogenic strain of Vibrio alginolyticus was tested for comparative purposes. Pseudomonas stutzeri and Candida utilis have no impact on survival, but enhance growth and total biomass production of the larvae. However, we noted that Pasteurella haemolityca affect negatively Artemia larvae. The adhesion and antagonism assay demonstrates that Candida utilis and Pseudomonas stutzeri are fairly adherent and play an important role in the enhancement of the protection of Artemia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemia; Probiotics; Adhesion; Yeast; Bacteria.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100017

Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap plant
Provedor de dados: 58
Autores: Silva,F.V.; Simões-Araújo,J.L.; Silva Júnior,J.P.; Xavier,G.R.; Rumjanek,N.G.
The aim of this work was to characterize rhizobia isolated from the root nodules of cowpea (Vigna unguiculata) plants cultivated in Amazon soils samples by means of ARDRA (Amplified rDNA Restriction Analysis) and sequencing analysis, to know their phylogenetic relationships. The 16S rRNA gene of rhizobia was amplified by PCR (polymerase chain reaction) using universal primers Y1 and Y3. The amplification products were analyzed by the restriction enzymes HinfI, MspI and DdeI and also sequenced with Y1, Y3 and six intermediate primers. The clustering analysis based on ARDRA profiles separated the Amazon isolates in three subgroups, which formed a group apart from the reference isolates of Bradyrhizobium japonicum and Bradyrhizobium elkanii. The clustering...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BNF; Bacteria; 16S rRNA; ARDRA; Sequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200033

Chlorine dioxide against bacteria and yeasts from the alcoholic fermentation
Provedor de dados: 58
Autores: Meneghin,Silvana Perissatto; Reis,Fabricia Cristina; Almeida,Paulo Garcia de; Ceccato-Antonini,Sandra Regina.
The ethanol production in Brazil is carried out by fed-batch or continuous process with cell recycle, in such way that bacterial contaminants are also recycled and may be troublesome due to the substrate competition. Addition of sulphuric acid when inoculum cells are washed can control the bacterial growth or alternatively biocides are used. This work aimed to verify the effect of chlorine dioxide, a well-known biocide for bacterial decontamination of water and equipments, against contaminant bacteria (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides) from alcoholic fermentation, through the method of minimum inhibitory concentration (MIC), as well as its effect on the industrial yeast inoculum. Lower MIC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorine dioxide; Bacteria; Yeast; Antibacterial agent; Alcohol; Fermentation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200026

The use of 16s rDNA methods in soil microbial ecology
Provedor de dados: 58
Autores: Macrae,Andrew.
New and exciting molecular methods, many using the 16S small sub-unit ribosomal nucleic acid molecule, are opening the microbial "black box" in soil. These studies have added much to our knowledge of microbial diversity in soils, and are beginning to advance our understanding of the relationship between this diversity and its function in soil processes. Over the next few years, the knowledge gained from molecular studies will, we hope, lead to improvements in sustainable land management and sustainable exploitation of soil genetic resources. As we enter the third millenium, it is appropriate to review the application of 16S rDNA methods to soil microbiology. This review examines 16S ribosomal DNA (rDNA) methods and their application to soil. It mentions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soil; Bacteria; Ecology; 16S rDNA.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200002

Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil
Provedor de dados: 58
Autores: Victor,Ligia Valéria; Cortelli,Sheila Cavalca; Aquino,Davi Romeiro; Carvalho Filho,Jonas de; Cortelli,José Roberto.
This cross-sectional study evaluated the periodontal status and the presence of periodontopathogens in 132 young, black ethnic subjects who live in Salvador/Bahia-Brazil and have never smoked. Periodontal Probing Depth (PPD), Clinical Attachment Level (CAL), Plaque Index (PI) and Gingival Index (GI) were measured and analyzed by ANOVA and Wilcoxon tests (p&lt;0.05) according to gender and age. The presence of A.actinomycetemcomitans, P.gingivalis, E.corrodens and F.nucleatum was determined by PCR and was analyzed by ANOVA, Wilcoxon, Student-t tests (p&lt;0.05). Mean values of PPD and CAL were 2.18 and 1.0mm, respectively. Clinical parameters did not show differences between subjects of varying gender and age. The microbial prevalence was observed to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African-Americans; Bacteria; Epidemiologic studies; Teen health; Periodontics.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200005

Optimization of polysaccharides production by bacteria isolated from soil
Provedor de dados: 58
Autores: Bueno,Silvia Messias; Garcia-Cruz,Crispin Humberto.
Six polysaccharide-producing bacteria, isolated from soil samples and identified as Pseudomonas and Arthrobacter (Strains 3B, 4B, 7B, 21B, 18E and 21D), were tested for the yield of polysaccharides produced during growth in two culture media: one containing glucose and the other sucrose (1, 2, 3, 4 and 5%). The yield was quantified measuring the viscosity of the broth, using the Poiscuille equation. The effect of temperature and pH of the culture media was investigated. The largest polysaccharide yield was obtained when the concentration of the carbon source was lower than 2%. Glucose and sucrose stimulated the polysaccharide production in a similar way. When the initial pH of the fermentation broth was increased from 5.0 up to 7.0, there was an increase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Soil; Polysaccharide production; Viscosity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300018

Conjunctival endogenous microbiota in patients submitted to cataract surgery
Provedor de dados: 58
Autores: Locatelli,Claudete I.; Kwitko,Sérgio; Simonetti,Amauri Braga.
Bacterial isolation, identification and antimicrobial susceptibility tests were carried out in ocular material collected with swab and polimethylmethacrylate (PMMA) or silicone intraocular lenses (IOL) from forty six patients submitted to cataract surgery. Seventy six isolates and seven different microorganisms were identified. Coagulase-negative staphylococci (CNS) were the predominant microorganisms isolated from swabs (71.4% of cases), PMMA lenses (81.3%) and silicon lenses (77.8%). Coagulase-negative staphylococci isolates revealed high resistance to penicillin G followed by tetracycline, chloramphenicol and aminoglicosides. However, these isolates displayed great susceptibility to vancomycin, cephalothin and ofloxacin. Except for penicillin G,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Conjunctiva; Cataract; Intraocular lens; Antibiotic.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300004

Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics
Provedor de dados: 58
Autores: El-Nakeeb,Moustafa A; Abou-Shleib,Hamida M; Khalil,Amal M; Omar,Hoda G; El-Halfawy,Omar M.
Several antihistaminics possess antibacterial activity against a broad spectrum of bacteria. However, the exact mechanism of such activity was unclear. Hence, the aim of this study is to investigate their mechanism of antibacterial activity especially their effect upon the permeability of the bacterial cytoplasmic membrane. The effects of azelastine, cetirizine, cyproheptadine and diphenhydramine were studied using Gram-positive and Gram-negative multiresistant clinical isolates. Leakage of 260 and 280 nm UV-absorbing materials was detected upon treatment with the tested antihistaminics; indicative of membrane alteration. Using an artificial membrane model, cholesterol-free negatively-charged unilamellar liposomes, confirmed the effect of antihistaminics...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antihistaminics; Azelastine; Bacteria; Cytoplasmic membrane; Liposomes; Permeability alteration.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300019

Bacterial diversity in rice-field water in Rio Grande do Sul
Provedor de dados: 58
Autores: Reche,Maria Helena Lima Ribeiro; Fiuza,Lídia Mariana.
Bacteria are the most diverse living beings on earth and only a fraction of them have been identified. This paper aims at the analysis of the diversity of mesophilic and heterotrophic bacteria found in samples of irrigation water from rice-fields areas in Rio Grande do Sul State, Brazil. Sample collection was carried out in five rice crop regions areas and followed by isolation, multiplication and bacterial characterization. The species' identification was based on biochemical tests (API-System, bioMérieux). Bacterial diversity was determined by the Shannon &amp;amp; Weaner and Pielou index. The results regarding bacterial diversity revealed 26 species. The most abundant representatives belonged to the Bacillus, Corynebacterium, Staphylococcus and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Diversity; Water; Rice-field.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300009

Histopathology of Anticarsia gemmatalis Hübner (Lepidoptera; Noctuidae) treated with Nucleopolyhedrovirus and Bacillus thuringiensis serovar kurstaki
Provedor de dados: 58
Autores: Knaak,Neiva; Fiuza,Lidia Mariana.
The Anticarsia gemmatalis is responsible for the use of chemical insecticides in the soybean culture, causing a significant increase in the costs of farming and a great unbalance in the ecosystem. The use of microbial agents, like Bacillus thuringiensis serovar kurstaki (Btk) and Anticarsia gemmatalis nucleopolyhedrovirus (AgNPV), they are an alternative to chemical control of the pest insects. In the interaction analysis of the entomopathogenic bacteria and virus it is considered important the in vitro action mode of these microbiology control agents. Therefore, the present study aims the histopathological analysis of the A. gemmatalis larvae digestive system after the interaction in vivo of the entomopathogenic Btk and AgNPV, represented the Dipel and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Virus; Bacteria; Biological control; Lepidoptera.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200017

Microbiota associated with chronic osteomyelitis of the jaws
Provedor de dados: 58
Autores: Gaetti-Jardim Júnior,Elerson; Fardin,Angélica Cristiane; Gaetti-Jardim,Ellen Cristina; Castro,Alvimar Lima de; Schweitzer,Christiane Marie; Avila-Campos,Mario Julio.
Chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteomyelitis; Bacteria; Anaerobes; Maxilla; Mandible.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400025

Hygienic-sanitary conditions of vegetables and irrigation water from kitchen gardens in the municipality of Campinas, SP
Provedor de dados: 58
Autores: Simões,Marise; Pisani,Beatriz; Marques,Eneida Gonçalves Lemes; Prandi,Maria Angela Garnica; Martini,Maria Helena; Chiarini,Paulo Flávio Teixeira; Antunes,José Leopoldo Ferreira; Nogueira,Ana Paula.
We examined samples of irrigation water and vegetables from kitchen gardens in Campinas, Brazil. The bacterial analysis condemned 22.3% of the vegetable samples, and the parasitological examination condemned 14.5%. The criteria established by the Brazilian legislation condemned 11.8% of the irrigation water samples. Parasites were significantly more frequent in vegetables in the rainy season, while excessive fecal coliforms were more frequent in the dry season. A proper monitoring of the irrigation water supply is important to avoid the contamination of vegetables.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vegetables; Irrigation water; Bacteria; Parasites.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400015

Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.
Provedor de dados: 58
Autores: De La Torre-Ruiz,Neyser; Ruiz-Valdiviezo,Víctor Manuel; Rincón-Molina,Clara Ivette; Rodríguez-Mendiola,Martha; Arias-Castro,Carlos; Gutiérrez-Miceli,Federico Antonio; Palomeque-Dominguez,Héctor; Rincón-Rosales,Reiner.
ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate, and had nitrogenase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agave; Inoculation; Bacteria; Inulin; 16S rRNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300587

Microbial populations of crude oil spill polluted soils at the Jordan-Iraq desert (the Badia region)
Provedor de dados: 58
Autores: Saadoun,Ismail; Mohammad,Munir J.; Hameed,Khalid M.; Shawaqfah,Mo'ayyad.
Microbial populations' inhabitants in crude petroleum contaminated soils were analyzed in relation with the soil characteristics. A noticeable greater decline of bacterial counts and diversity but a prevalence of the genus Pseudomonas over the other identified genera in the fresh contaminated soils as compared to the old ones was observed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Crude oil; Fungi; Soil; Streptomycetes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300008

Grazing of particle-associated bacteria-an elimination of the non-viable fraction
Provedor de dados: 58
Autores: Gonsalves,Maria-Judith; Fernandes,Sheryl Oliveira; Priya,Madasamy Lakshmi; LokaBharathi,Ponnapakkam Adikesavan.
Abstract Quantification of bacteria being grazed by microzooplankton is gaining importance since they serve as energy subsidies for higher trophic levels which consequently influence fish production. Hence, grazing pressure on viable and non-viable fraction of free and particle-associated bacteria in a tropical estuary controlled mainly by protist grazers was estimated using the seawater dilution technique. In vitro incubations over a period of 42 h showed that at the end of 24 h, growth coefficient (k) of particle-associated bacteria was 9 times higher at 0.546 than that of free forms. Further, ‘k’ value of viable cells on particles was double that of free forms at 0.016 and 0.007, respectively. While bacteria associated with particles were grazed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Ciliates; Grazing; Particle; Protists; Viable.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100037

Cloning and characterization of the gene encoding the PepF endopeptidase from the aquatic bacterium Caulobacter crescentus
Provedor de dados: 58
Autores: Braz,Vânia S.; Lang,Elza A.S.; Marques,Marilis V.
The metallopeptidases have a very important role in bacteria, being involved in several processes that rely on protein turnover, such as nutrition, degradation of signal peptides, protein localization and virulence. We have cloned and characterized the gene of the metalloendopeptidase PepF from the aquatic bacterium Caulobacter crescentus. The gene upstream of pepF (orf1) encodes a conserved hypothetical protein found in Mycobacterium and Streptomyces. pepF is co-transcribed with the gene downstream (orf3), which encodes a protein that belongs to the ABC1 protein kinase family, suggesting that these two proteins may share a common function in the cell. The C. crescentus PepF protein possesses the conserved HEXGH motif present in zinc binding domains of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endopeptidase; M3 family; Gene regulation; Bacteria.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100017

Detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from diamondback moth, Plutella xylostella (Linnaeus), from India and its possible role in indoxacarb degradation
Provedor de dados: 58
Autores: Ramya,Shanivarsanthe Leelesh; Venkatesan,Thiruvengadam; Srinivasa Murthy,Kottilingam; Jalali,Sushil Kumar; Verghese,Abraham.
Abstract Diamondback moth (DBM), Plutella xylostella (Linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. The insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. The present study aimed to determine the diversity of gut microflora in DBM, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. We screened 11 geographic populations of DBM in India and analyzed them for bacterial diversity. The culturable gut bacterial flora underwent molecular characterization with 16S rRNA. We obtained 25 bacterial isolates from larvae (n = 13) and adults (n = 12) of DBM. In larval gut isolates,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plutella xylostella; Bacteria; 16S rRNA; Phylogeny; Carboxylesterase; Indoxacarb.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200327

Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
Provedor de dados: 58
Autores: Muthukamalam,Santhakumar; Sivagangavathi,Sivalingam; Dhrishya,Dharmapal; Sudha Rani,Sadras.
ABSTRACT Role of microbes in bioremediation of oil spills has become inevitable owing to their eco friendly nature. This study focused on the isolation and characterization of bacterial strains with superior oil degrading potential from crude-oil contaminated soil. Three such bacterial strains were selected and subsequently identified by 16S rRNA gene sequence analysis as Corynebacterium aurimucosum, Acinetobacter baumannii and Microbacterium hydrocarbonoxydans respectively. The specific activity of catechol 1,2 dioxygenase (C12O) and catechol 2,3 dioxygenase (C23O) was determined in these three strains wherein the activity of C12O was more than that of C23O. Among the three strains, Microbacterium hydrocarbonoxydans exhibited superior crude oil degrading...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crude oil; Biodegradation; Bacteria; Catechol dioxygenase; Biosurfactant.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400637

Biological activity of the essential oils from Cinnamodendron dinisii and Siparuna guianensis
Provedor de dados: 58
Autores: Andrade,Milene Aparecida; Cardoso,Maria das Graças; Gomes,Marcos de Souza; Azeredo,Camila Maria Oliveira de; Batista,Luís Roberto; Soares,Maurilio José; Rodrigues,Leonardo Milani Avelar; Figueiredo,Ana Cristina S.
This study had analyzed the antibacterial, antifungal and trypanocidal activity of the essential oils from Cinnamodendron dinisii Schwacke (Canellaceae) and Siparuna guianensis Aublet (Siparunaceae). The essential oils were obtained from fresh leaves by hydrodistillation, using a modified Clevenger apparatus. Chemical analysis by gas-liquid chromatography coupled to mass spectrometry (GC-MS) showed that these essential oils are rich in monoterpene and sesquiterpene hydrocarbons. Activity against the pathogenic bacteria Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella choleraesuis and Staphylococcus aureus was evaluated with the agar cavity diffusion method, while activity on the filamentous fungi Aspergillus flavus, Aspergillus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Volatile oils; Fungi; Bacteria; Trypanosoma cruzi.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100189

Antibacterial activity of four mouthrinses containing triclosan against salivary Staphylococcus aureus
Provedor de dados: 58
Autores: Tanomaru,Juliane Maria Guerreiro; Nascimento,Andresa Piacezzi; Watanabe,Evandro; Matoba-Júnior,Fumio; Tanomaru-Filho,Mário; Ito,Izabel Yoko.
The maximum inhibitory dilution (MID) of triclosan-based mouthwashes against 28 Staphylococcus aureus strains was evaluated. Dilutions ranging from 1/10 to 1/655,360 were prepared. Strains were inoculated using a Steers multipoint inoculator. The MID was considered as the maximum dilution capable of inhibiting microorganism growth. The mouthwashes presented different MIDs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microorganism; Bacteria; Mouthwash; Triclosan.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300030

Nasal, oral and rectal microbiota of Black lion tamarins (Leontopithecus chrysopygus)
Provedor de dados: 58
Autores: Carvalho,Vania M.; Vanstreels,Ralph E.T.; Paula,Cátia D.; Kolesnikovas,Cristiane K.M.; Ramos,Maria Christina C.; Coutinho,Selene D.; Martins,Cristiana S.; Pissinatti,Alcides; Catão-Dias,José L.
Black lion tamarins (Leontopithecus chrysopygus) are endangered callithrichids. Their conservation may require future translocations or reintroductions; however these approaches involve risks of pathogen introduction in the environment and stress-related opportunistic infections in these animals. In order to screen for opportunistic and potential pathogenic bacterial and fungal microbiota, ten free-ranging and ten captive Black lion tamarins were studied and the results compared. Nasal, oral and rectal swabs were collected and cultured for aerobic and facultative anaerobic bacteria and fungi, and a total 203 bacterial and 84 fungal isolates were obtained. Overall, the most frequent organisms were Staphylococcus spp., Bacillus spp., Candida spp. and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbiota; Bacteria; Fungi; Black lion tamarins; Leontopithecus chrysopygus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400051

Microbial diversity in Brazilian mangrove sediments: a mini review
Provedor de dados: 58
Autores: Ghizelini,Angela Michelato; Mendonça-Hagler,Leda Cristina Santana; Macrae,Andrew.
The importance and protection of mangrove ecosystems has been recognized in Brazilian Federal law since 1965. Being protected in law, however, has not always guaranteed their protection in practice. Mangroves are found in coastal and estuarine locations, which are prime real estate for the growth of cities, ports and other economic activities important for Brazilian development. In this mini-review we introduce what mangroves are and why they are so important. We give a brief overview of the microbial diversity found in mangrove sediments and then focus on diversity studies from Brazilian mangroves. We highlight the breadth and depth of knowledge about mangrove microbial communities gained from studying Brazilian mangroves. We report on the exciting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangroves; Biodiversity; Bacteria; Fungi; Brazil; Conservation; Ecology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400002

Effects of different osmolarities on bacterial biofilm formation
Provedor de dados: 58
Autores: Kavamura,Vanessa Nessner; Melo,Itamar Soares de.
Biofilm formation depends on several factors. The influence of different osmolarities on bacterial biofilm formation was studied. Two strains (Enterobacter sp. and Stenotrophomonas sp.) exhibited the most remarkable alterations. Biofilm formation is an important trait and its use has been associated to the protection of organisms against environmental stresses.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Biofilm formation; NaCl; D-sorbitol.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200034

Bioluminescent bacteria: lux genes as environmental biosensors
Provedor de dados: 58
Autores: Nunes-Halldorson,Vânia da Silva; Duran,Norma Letícia.
Bioluminescent bacteria are widespread in natural environments. Over the years, many researchers have been studying the physiology, biochemistry and genetic control of bacterial bioluminescence. These discoveries have revolutionized the area of Environmental Microbiology through the use of luminescent genes as biosensors for environmental studies. This paper will review the chronology of scientific discoveries on bacterial bioluminescence and the current applications of bioluminescence in environmental studies, with special emphasis on the Microtox toxicity bioassay. Also, the general ecological significance of bioluminescence will be addressed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Bioluminescence; Lux genes; Microtox toxicity bioassay.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200001

Sensitivity of spoiling and pathogen food-related bacteria to Origanum vulgare L. (Lamiaceae) essential oil
Provedor de dados: 58
Autores: Souza,Evandro Leite de; Stamford,Tânia Lúcia Montenegro; Lima,Edeltrudes de Oliveira.
Origanum vulgare L. (oregano), Lamiaceae, has been known as plant specie with prominent biological properties for a long time. This study aimed to evaluate the antibacterial activity of Origanum vulgare essential oil on various Gram-positive and Gram-negative spoiling and/or pathogen food-related bacteria, as well as to observe its antimicrobial effectiveness in a food conservation micromodel. The results showed a strong antibacterial activity of the assayed essential oil noted by large growth inhibition zones (30-37 mm). MIC values were between 20-40 µL/mL for the most bacteria strains. Essential oil was able to cause significant (P&lt;0.05) inhibitory effect on the bacteria viability providing a bacteriostatic effect after 24hours of exposure. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Origanum vulgare L.; Essential oil; Antimicrobial activity; Bacteria.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400023

The reaction of dental pulp to Escherichia coli lipopolysaccharide and Enterococcus faecalis lipoteichoic acid
Provedor de dados: 58
Autores: Oliveira,Laudimar Alves de; Barbosa,Sérgio Valmor.
This research evaluates the effects of the lipopolysaccharides (LPS) from Escherichia coli and lipoteichoic acid (LTA) from Enterococcus faecalis on dental pulp. These molecules are components of the Gram-negative and Gram-positive bacteria cell wall, respectively. Ten dogs were used in the experiment. Inoculation in surgically opened pulp and coronal restoration with glass ionomer was the method chosen. The evaluation times were 1, 7, 15, 30 and 60 days. The results showed that the LPS and LTA, at 150 µg/ml, produced a negative interference in the pulp leading to destruction. LTA caused less damage than LPS.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipopolysaccharide; Endotoxin; Lipoteichoic acid; Dental pulp; Bacteria.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200018

Detection of Salmonella spp, Salmonella Enteritidis and Typhimurium in naturally infected broiler chickens by a multiplex PCR-based assay
Provedor de dados: 58
Autores: Paião,F.G.; Arisitides,L.G.A.; Murate,L.S.; Vilas-Bôas,G.T.; Vilas-Boas,L.A.; Shimokomaki,M.
The presence of Salmonella in the intestinal tract, on the chickens skin and among their feathers, may cause carcasses contamination during slaughtering and processing and possibly it is responsible by the introduction of this microorganism in the slaughterhouses. A rapid method to identify and monitor Salmonella and their sorovars in farm is becoming necessary. A pre-enriched multiplex polymerase chain reaction (m-PCR) assay employing specific primers was developed and used to detect Salmonella at the genus level and to identify the Salmonella enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica serovar Typhimurium (S. Typhimurium) in broiler chicken swab samples. The method was validated by testing DNA extract from 90 fresh culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aviculture; Bacteria; Detection.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100005

Propolis antimicrobial activity against periodontopathic bacteria
Provedor de dados: 58
Autores: Gebara,Elaine C.E.; Lima,Luiz A.; Mayer,Marcia P.A.
Propolis extract antimicrobial activity against periodontopathic (ATCC) bacteria was investigated "in vitro". Bacterial strains tested were: Prevotella intermedia, Prevotella melaninogenica, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Capnocytophaga gingivalis and Fusobacterium nucleatum. Minimal inhibitory concentration (MIC) for the strains tested was determined using the method of broth dilution with the propolis extract in serial concentrations. Results showed MIC of 1 µg/ml for Actinobacillus actinomycetemcomitans and Capnocytophaga gingivalis; and 0.25 µg/ml for Prevotella intermedia, Prevotella melaninogenica, Porphyromonas gingivalis and Fusobacterium nucleatum. Some superinfectant organisms were also tested: Candida albicans...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Periodontal pathogens; Periodontal diseases; Minimal inhibitory concentration; Propolis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400018

Direct identification of bovine mastitis pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in pre-incubated milk
Provedor de dados: 58
Autores: Barreiro,Juliana R.; Gonçalves,Juliano L.; Grenfell,Rafaella; Leite,Renata F.; Juliano,Luiz; Santos,Marcos V.
ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow. For direct identification method, quarter milk samples (n = 120) were skimmed by centrifugation (10,000 × g/10 min) and pre-incubated at 37 ºC for 12 h. After pre-incubation, quarter milk samples were submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk; Bacteria; Mastitis; Mass spectrometry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400801

Identification of bacterial agents of enteric diseases by multiplex PCR in growing-finishing pigs
Provedor de dados: 58
Autores: Baccaro,Maria Regina; Moreno,Andrea Micke; Shinya,Luciane Tieko; Dotto,Daniela Sabatini.
In Brazil, the most common bacterial enteric diseases affecting growing and finishing pigs are porcine proliferative enteritis, porcine intestinal spirochetosis, swine dysentery, and salmonellosis. The diagnosis of these diseases by routine culture techniques is expensive, difficult, time-consuming, and even impossible, in cases of porcine proliferative enteritis. The detection of pathogens by polymerase chain reaction is a highly sensitive and specific method that can be an useful tool in veterinary diagnosis. Two multiplex PCR (M-PCR) assays were tested for simultaneous detection and identification of bacterial agents associated with porcine proliferative enteritis, porcine intestinal spirochetosis, swine dysentery, and salmonellosis in diarrheic fecal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteritis; PCR; Pig; Bacteria; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300008

Bacterial diversity in relatively pristine and anthropogenically-influenced mangrove ecosystems (Goa, India)
Provedor de dados: 58
Autores: Fernandes,Sheryl Oliveira; Kirchman,David L.; Michotey,Valérie D.; Bonin,Patricia C.; LokaBharathi,P.A.
To appreciate differences in benthic bacterial community composition at the relatively pristine Tuvem and the anthropogenically-influenced Divar mangrove ecosystems in Goa, India, parallel tag sequencing of the V6 region of 16S rDNA was carried out. We hypothesize that availability of extraneously-derived anthropogenic substrates could act as a stimulatant but not a deterrent to promote higher bacterial diversity at Divar. Our observations revealed that the phylum Proteobacteria was dominant at both locations comprising 43-46% of total tags. The Tuvem ecosystem was characterized by an abundance of members belonging to the class Deltaproteobacteria (21%), ~ 2100 phylotypes and 1561 operational taxonomic units (OTUs) sharing &amp;gt; 97% similarity. At...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Denitrification; Diversity; Mangroves; Sediments.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400006

Multiple responses of gram-positive and gram-negative bacteria to mixture of hydrocarbons
Provedor de dados: 58
Autores: Lăzăroaie,Mihaela Marilena.
Most of our knowledge about pollutants and the way they are biodegraded in the environment has previously been shaped by laboratory studies using hydrocarbon-degrading bacterial strains isolated from polluted sites. In present study Gram-positive (Mycobacterium sp. IBB Po1, Oerskovia sp. IBB Po2, Corynebacterium sp. IBB Po3) and Gram-negative (Chryseomonas sp. IBB Po7, Pseudomonas sp. IBB Po10, Burkholderia sp. IBB Po12) bacteria, isolated from oily sludge, were found to be able to tolerate pure and mixture of saturated hydrocarbons, as well as pure and mixture of monoaromatic and polyaromatic hydrocarbons. Isolated Gram-negative bacteria were more tolerant to mixture of saturated (n-hexane, n-hexadecane, cyclohexane), monoaromatic (benzene, toluene,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Multiple response; Mixture of hydrocarbons.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300016

A conservative region of the mercuric reductase gene (merA) as a molecular marker of bacterial mercury resistance
Provedor de dados: 58
Autores: Sotero-Martins,Adriana; Jesus,Michele Silva de; Lacerda,Michele; Moreira,Josino Costa; Filgueiras,Ana Luzia Lauria; Barrocas,Paulo Rubens Guimarães.
The most common bacterial mercury resistance mechanism is based on the reduction of Hg(II) to Hg0, which is dependent of the mercuric reductase enzyme (MerA) activity. The use of a 431 bp fragment of a conservative region of the mercuric reductase (merA) gene was applied as a molecular marker of this mechanism, allowing the identification of mercury resistant bacterial strains.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MerA gene; Mercury resistant; Bacteria; Molecular marker.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200020

Employing pharmacokinetic and pharmacodynamic principles to optimize antimicrobial treatment in the face of emerging resistance
Provedor de dados: 58
Autores: Santos Filho,Lauro; Kuti,Joseph L.; Nicolau,David P.
Antimicrobial efficacy in vivo is not exclusively defined by the activity of an antibiotic as determined in the in vitro susceptibility test. Knowledge of the pharmacokinetics and pharmacodynamics of antimicrobials and all phenomena occurring between antimicrobial agents and microorganisms is imperative. The pharmacodynamic (PD) parameters most often used in studies of antibiotic effect include the following relationships: the maximum free concentration (fCmax) to minimum inhibitory concentration (MIC) ratio, the free area under the curve (fAUC/MIC) ratio and the duration of time the free concentration exceeds the MIC (fT&gt;MIC). Utilization of known pharmacokinetic/ pharmacodynamic surrogate relationships should help to optimize treatment outcome,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic; Bacteria; Pharmacodynamics; Resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200001

Glycodendrimers: versatile tools for nanotechnology
Provedor de dados: 60
Autores: Roy,René; Shiao,Tze Chieh; Rittenhouse-Olson,Kate.
Combining nanotechnology with glycobiology has triggered an exponential growth of research activities in the design of novel functional bionanomaterials (glyconanotechnology). More specifically, recent synthetic advances towards the tailored and versatile design of glycosylated nanoparticles namely glyconanoparticles, considered as synthetic mimetics of natural glycoconjugates, paved the way toward diverse biomedical applications. The accessibility of a wide variety of these structured nanosystems, in terms of shapes, sizes, and organized around stable nanoparticles have readily contributed to their development and applications in nanomedicine. In this context, glycosylated gold-nanoparticles (GNPs), glycosylated quantum dots (QDs), fullerenes, single-wall...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrimer; Glycodendrimer; Vaccine; Bacteria; Nanoparticles; E. coli; Lanthanides.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700008

Drugs obtained by biotechnology processing
Provedor de dados: 60
Autores: Almeida,Hugo; Amaral,Maria Helena; Lobão,Paulo.
In recent years, the number of drugs of biotechnological origin available for many different diseases has increased exponentially, including different types of cancer, diabetes mellitus, infectious diseases (e.g. AIDS Virus / HIV) as well as cardiovascular, neurological, respiratory, and autoimmune diseases, among others. The pharmaceutical industry has used different technologies to obtain new and promising active ingredients, as exemplified by the fermentation technique, recombinant DNA technique and the hybridoma technique. The expiry of the patents of the first drugs of biotechnological origin and the consequent emergence of biosimilar products, have posed various questions to health authorities worldwide regarding the definition, framework, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Recombinant DNA; Hybridoma technique; Bacteria; Antibiotics; Vaccines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200002

Fecal Contamination of Groundwater in a Small Rural Dryland Watershed in Central Chile
Provedor de dados: 117
Autores: Valenzuela,Mariela; Lagos,Bernardo; Claret,Marcelino; Mondaca,María A; Pérez,Claudio; Parra,Oscar.
Research on microbiological groundwater quality was conducted in Chile in a rural watershed that has almost no other water source. Forty-two wells were randomly selected and levels of indicator bacteria - total coliforms (TC), fecal coliforms (FC), and fecal streptococci (FS) - were repeatedly measured during the four seasons of 2005. The aim of this study was to characterize microbiological groundwater quality, relate indicator levels to certain watershed features and management characteristics which are likely to affect water quality. The dynamics of seasonal temporal contamination was determined with statistical analyses of indicator organism concentrations. Nonparametric tests were used to analyze relationships between bacterial indicators in well...
Tipo: Journal article
Palavras-chave: Biological contamination; Bacteria; Water quality; Environmental pollution.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200013

Microbial additives in the composting process
Provedor de dados: 64
Autores: Ribeiro,Noelly de Queiroz; Souza,Thiago Pereira; Costa,Lívia Martinez Abreu Soares; Castro,Cibelli Paula de; Dias,Eustáquio Souza.
ABSTRACT Composting is the process of natural degradation of organic matter carried out by environmental microorganisms whose metabolic activities cause the mineralization and partial humification of substances in the pile. This compost can be beneficially applied to the soil as organic fertilizer in horticulture and agriculture. The number of studies involving microbial inoculants has been growing, and they aim to improve processes such as composting. However, the behavior of these inoculants and other microorganisms during the composting process have not yet been described. In this context, this work aimed to investigate the effects of using a microbial inoculum that can improve the composting process and to follow the bacterial population dynamics...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High Resolution Melt (HRM); Bacteria; Microbial ecology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000200159

SENSORY ASPECTS AND REDUCTION OF Salmonella IN IRRADIATED EGG POWDER
Provedor de dados: 64
Autores: Froehlich,Ângela; Franco,Bernadette Dora Gombossy de Melo; Destro,Maria Teresa; Landgraf,Mariza.
ABSTRACTEggs and their products have been implicated in food-borne disease outbreaks due to contamination with SalmonellaEnteritidis. Irradiation, as a food preservation technology, could minimize such contamination. The aim of this study was to determine the effects of irradiation in powdered egg yolk, egg white, and whole egg samples spiked with Salmonella Enteritidis. The powdered egg samples were exposed to doses of 0.5, 1.0, 1.0, 2.0, 2.5, 3.0, 3.5, or 4.0 kGy irradiation. Odors and tastes of raw and cooked non-inoculated, irradiated samples, and non-irradiated samples, were analyzed by a trained panel. A dose of 2.0 kGy resulted in slight to moderate changes in powdered egg yolk samples, compared to non-irradiated controls, while minimal differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Elimination; Powder food.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500506