Consulta: subjectFacets:"Baculovirus"
Registros recuperados: 34
Data/hora: 18/06/2024 14:07:09

Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture
Provedor de dados: 69
Autores: Batista,Fabiana R. X.; Pereira,Carlos A.; Mendonça,Ronaldo Z.; Moraes,Angela M.
Insect cell culture has become increasingly useful for the production of heterologous proteins as well as of baculovirus polyhedra, and several different culture media formulations can be employed for this purpose. The goal of this work was to assess the potential of lyophylized milk whey ultra filtration retentate (MWR) when associated to yeast extract (YE), glucose and Pluronic F68 (PF68) to partially replace fetal calf serum (FBS) in Spodoptera frugiperda Sf9 cells culture in Grace's medium, aiming AgMNPV baculovirus production. Batch cultivation results showed that the yeast extract and the milk whey concentrate effectively increased cell concentration to about half the level commonly verified for the Sf900II serum-free medium. A 4-fold increase in...
Tipo: Journal article
Palavras-chave: Baculovirus; Concentrated milk whey; Grace's medium; Insect cells; Media formulation; Spodoptera frugiperda Sf9.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500007

Analise de DNA do virus da lagarta-da-soja (AgNPV): estudo comparativo entre quinze isolados geograficos.
Provedor de dados: 14
Autores: CASTRO, M. E. B. de; SOUZA, M. L. de; SIHLER, W.; OLIVEIRA, M. D. de; NAKAMURA, E. S.
bitstream/item/90531/1/1715-Analise-de-DNA.pdf
Tipo: Outras publicações técnicas (INFOTECA-E)
Palavras-chave: Bioinseticida; Praga %% Plant; Weed; Bioinseticide.; Anticarsia Gemmatalis; Baculovirus; Planta.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/174564

Controle biológico da lagarta-do-cartucho, Spodoptera frugiperda, com o baculovírus.
Provedor de dados: 14
Autores: VALICENTE, F. H.; CRUZ, I.
Uso do baculovirus; Modo de atuacao do baculovirus; Descricao e biologia da praga; Quando recomendar o controle; Resultados de pesquisa com o baculovirus na EMBRAPA/CNPMS; Resultados de laboratorio; Resultados de aplicacoes no campo; Recomendacoes para o uso do baculovirus no controle da lagarta do cartucho; Epoca de aplicacao; Tamanho da lagarta; Pulverizacao; Hora de aplicacao.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Lagarta-do-cartucho; Pest.; Baculovirus; Controle Biológico; Milho; Praga; Spodoptera Frugiperda.; Biological control.
Ano: 1991
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/471750

Análise filogenética de Condylorrhiza vestigialis MNPV baseada no gene de virulência p74.
Provedor de dados: 14
Autores: ALMEIDA, G. F.; PAULA, D. P.; RIBEIRO, Z. M. de A.; SOUZA, M. L.; CASTRO, M. E. B.
bitstream/CENARGEN-2009-09/31586/3/bp235_1208.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Condylorrhiza vestigialis; MNPV; Melhoramento genético; P74.; Baculovirus; Controle Biológico; Lagarta; Vírus.
Ano: 2008
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/191235

Controle de artrópodes-praga com vírus entomopatogênicos.
Provedor de dados: 14
Autores: CASTRO, M. E. B. de; RIBEIRO, B. M.; CRAVEIRO, S. R.; INGLIS, P. W.; VALICENTE, F. H.
Características biológicas e ecológicas; Taxonomia, diversidade e evolução; Modo de ação e ciclo biológico dos baculovírus; Patogênese e interação vírus-hospedeiro; Uso dos baculovírus e sua importância; Desafios e perspectivas.
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Vírus; Baculovirus; Taxonomia; Controle Biológico.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1122097

Identificação do baculovirus da lagarta-do-álamo Condylorrhiza vestigialis (Guenée, 1854) (Lepidoptera: Pyralidae).
Provedor de dados: 14
Autores: CASTRO, M. E. B.; RIBEIRO, Z. M. A.; SOUZA, M. L.; SOUSA, N. J.; MOSCARDI, F.
bitstream/CENARGEN/24081/1/cot087.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Lagarta do álamo; Condylorrhiza vestigialis.; Baculovirus; Identificação; Patógeno; Praga de Planta.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/185003

Controle biológico da lagarta do cartucho, Spodoptera frugiperda, com baculovírus.
Provedor de dados: 14
Autores: VALICENTE, F. H.; TUELHER, E. de S.
bitstream/CNPMS-2010/22431/1/Circ-114.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Baculovirus; Controle Biológico; Milho; Spodoptera Frugiperda.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/660134

Controle da lagarta da soja por Baculovirus.
Provedor de dados: 14
Autores: MOSCARDI, F.
O que é o Baculovírus; Como age o Baculovírus; Como o Baculovirus pode ser utilizado; Preparo da amostra; Aplicação; Coleta das lagartas; Armazenamento; Utilização do Baculovírus formulado; Vantagem do uso do Baculovírus; Lembretes importantes.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Inseto praga; Brasil; Parana; Insect pest; Soybean.; Anticarsia Gemmatalis; Baculovirus; Controle Biológico; Soja.; Biological control.; Brazil.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/445136

Avaliação do efeito do Baculovirus anticarsia sobre Podisus nigrispinus (DALLAS, 1851), predador da lagarta da soja Anticarsia gemmatalis (HUBNER, 1818).
Provedor de dados: 14
Autores: WATANABE, M. A.; NARDO, E. A. B. de; MORAES, G. J. de; MARIGO, A. L. S.
Este trabalho tem como objetivo conhecer os efeitos da ingestão de lagartas da soja infectadas com B. anticarsia pelo predador Podisus nigrispinus, avaliando-se os parâmetros biológicos de cada inseto.
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Baculovirus; Lagarta; Soja.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/14562

Prospecção de genes de virulência em variantes genotípicos de Anticarsia gemmatalis MNPV.
Provedor de dados: 14
Autores: FERREIRA, B. C.; CASTRO, M. E. B. de; PINEDO, J. F. R.; SIHLER, W.; SOUZA, M. L. de.
bitstream/CENARGEN-2009-09/31584/3/bp233_1208.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Genótipo MNPV.; Baculovirus; Anticarsia Gemmatalis; Controle Biológico; Lagarta.
Ano: 2008
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/191233

Análise da atividade anti-apoptótica do gene iap-3 de Anticarsia gemmatalis MNPV.
Provedor de dados: 14
Autores: SOARES, E. F.; RIBEIRO, CASTRO, M. E. B. de.
bitstream/CENARGEN/24245/1/bp042.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Gene anti-apoptótico; Apoptose; Células UFL-AG-286.; Baculovirus; Biologia Molecular; Anticarsia Gemmatalis; Controle Biológico.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/185132

Avaliação de diferentes parâmetros para potencializar a produção massal in vivo de baculovírus ChinNPV na lagarta falsa-medideira da soja (Chrysodeixis includens).
Provedor de dados: 14
Autores: SANCHES, M. M.; SILVA, C. E. P.; SANTOS, A. L. R. dos; BENITO, N. P.; SIHLER, W.; SOUZA, M. L. de.
bitstream/item/216406/1/Boletim-Baculovirus-362final.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Chrysodeixis includens nucleopolyhedrovirus; Produção in vivo; In vivo production; Lagarta-falsa-medideira; Baculovirus; Controle Biológico.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1125281

Instruções para a difusão do emprego, obtenção, coleta e armazenamento de Baculovirus anticarsia com vistas ao controle da lagarta da soja no MS.
Provedor de dados: 14
Autores: GOMEZ, S. A.; GAZZONI, D. L.
bitstream/item/39160/1/COT-16.-84.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Coleta; Brasil; Mato Grosso do Sul; Soybean; Pests of plants; Production.; Baculovirus; Armazenamento; Baculovirus Anticarsia; Controle Biológico; Cerrado; Glycine Max; Praga de Planta; Produção; Soja; Vírus.; Brazil; Biological control.
Ano: 1984
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/232413

Análise da estabilidade genética do Erinnyis ello granulovirus aplicado em Santa Catarina como bioinseticida no período de 1986 a 2000.
Provedor de dados: 14
Autores: COSTA, N. R.; CASTRO, M. E. B. de; SIHLER, W.; PEGORARO, R. A.; SOUZA, M. L. de.
bitstream/CENARGEN/27330/1/bp110.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Bioinseticida; Praga da mandioca; Mandarová da mandioca; Estabilidade genética; Monitoramento; Santa catarina; Brasil.; Baculovirus; Controle Biológico.; Erinnyis ello granulovirus.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/185880

Differentiation of BP-type baculovirus strains using in situ hybridization
Provedor de dados: 5
Autores: Durand, S; Lightner, Dv; Bonami, Jr.
BP-type viruses infect wild and farmed shrimp species in the Americas and can cause high mortality in larval stages. Histologically, BP disease is characterized by the presence of occlusion bodies (OBs) in the nucleus of hepatopancreatic epithelial cells. By in situ hybridization using different molecular probes, we tested shrimp infected with BP-type viruses collected from several geographical areas. In one shrimp, a case of double infection by 2 different BP-type viruses was noted. This has not been previously reported. The molecular probes recognized only the Pacific strain of BP. This specificity of the probe was confirmed by in situ hybridization tests with some infected shrimp collected from the Atlantic and Pacific coasts. The probes reacted only...
Tipo: Text
Palavras-chave: Baculovirus; BP; In situ hybridization; Nonradioactive probes; Shrimp.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/00447/55821/62253.pdf

Régulation de la transcription de gènes précoces exprimés par le Baculovirus AcMNPV dans des cellules d'insectes
Provedor de dados: 5
Autores: Ohresser, Marc.
La transformation stable de cellules SF9 de Lépidoptère a été mise au point et l'efficacité de différents vecteurs d'expression a été comparée. Cela nous a permis de mettre en évidence un activateur transcriptionnel sur le génome du densovirusJcDNV. Nous avons fait exprimer des ARN anti-sens artificiels dirigés contre des gènes d 'AcMNPV dans les cellules SF9. Nous avons mis en évidence des ARN transcrits en sens inverse sur le promoteur du gène de l'ADN polymérase (dnapol) du baculovirus AcMNPV, mais il a été établi qu'ils n'avaient aucun effet sur la transcription du gène dnapol. Alors, nous avons déterminé que ces ARN avaient la capacité d'être traduits en une protéine dont la fonction reste inconnue.
Tipo: Text
Palavras-chave: Cellules d'insectes; Transformation stable; Densovirus; ARN anti-sens; Baculovirus; Gènes précoces; ADN polymerase.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00439/55096/56539.pdf

Field efficacy of the nucleopolyhedrovirus of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae): effect of formulations, water pH, volume and time of application, and type of spray nozzle
Provedor de dados: 82
Autores: SILVA,MAURO TADEU BRAGA; MOSCARDI,FLÁVIO.
The efficacy of the nucleopolyhedrovirus of Anticarsia gemmatalis Hübner (AgMNPV) was evaluated in relation to different application parameters of this biological insecticide, to elucidate some problems of its low efficacy in certain regions in Brazil, mainly in the state of Rio Grande do Sul (RS). Each experiment was conducted during four consecutive soybean seasons (1994/95 to 97/98), in Cruz Alta -- RS, to evaluate the following parameters on AgMNPV field efficacy: available commercial formulations of the virus; pH of the viral aqueous suspension; spray volume; application time; spray nozzle type; and the mixture of the virus with mineral oil. Each plot consisted of 28 soybean rows of 10 m in length, spaced of 40 cm. The statistical design was the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Velvetbean caterpillar; Biological control; Baculovirus; Soybean.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2002000100011

Nucleopolyhedrovirus: scanning electron microscopy technique
Provedor de dados: 82
Autores: Torquato,Ednéia F.B.; Miranda Neto,Marcílio H. de; Brancalhão,Rose M.C.; Franco,Valdeni S.
A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis reveled a well defined morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; Bombyx mori; BmMNPV; Polyhedra; Truncated octahedron.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600011

Expression of P30, The Major Surface Antigen of Toxoplasma gondii in Baculovirus-insect System and The Evaluation of Immune Response Induced by P30
Provedor de dados: 19
Autores: Chen, Xiao-Guang; Ma, Xin; Wu, Kun; Peng, Hong-Juan; Shen, Shu-Man; Liu, Guo-Zhang; Chan, Siu-Ming.
Palavras-chave: Toxoplasma gondii; Major surface antigen; Baculovirus; Expression system; Immune.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/275

Detection of Antibodies to Babesia equi by The Latex Agglutination Test with Recombinant Merozoite Antigen-2
Provedor de dados: 19
Autores: Tanaka, Tetsuya; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; EMA-2; Baculovirus; Latex agglutination test.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/329

In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
Provedor de dados: 86
Autores: Sihler,William; Souza,Marlinda Lobo de; Valicente,Fernando Hercos; Falcão,Rosana; Sanches,Marcio Martinello.
Abstract: The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; Biological control; Cell culture; Fall armyworm; In vitro production; SfMNPV.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100001

Efficacy of microencapsulated nucleopolyhedroviruses from Colombia as biological insecticides against Spodoptera frugiperda (Lepidoptera: Noctuidae)
Provedor de dados: 109
Autores: Barrera-Cubillos,Gloria Patricia; Gómez-Valderrama,Juliana Andrea; Rivero,Laura Fernanda Villamizar.
Abstract: Wettable powder formulations by microencapsulation of viral occlusion bodies (OBs) of both Spodoptera frugiperda multiple nucleopolyhedrovirus from Colombia (SfCOL) and a genotypic variant (SfCOL-A) were evaluated for controlling the fall armyworm S. frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in maize crops. Microencapsulation preserved OBs activity after three months of storage at 35ºC, where insecticidal activity loss was not greater than 12%. Additionally, the formulation protected the OBs against inactivation caused by UV-B radiation, retaining its insecticidal activity after 6 hours of UV laboratory exposure, in contrast to unformulated viral suspensions, which presented an Original Activity Remaining (OAR) between 12.1 and 50%. Under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microencapsulation; Baculovirus; Spodoptera frugiperda; Biological control; Maize.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000200267

Interactions among insect-resistant soybean genotypes extracts with populations of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) susceptible and resistant to its nucleopolyhedrovirus
Provedor de dados: 42
Autores: Piubelli,Giorla C.; Moscardi,Flávio; Hoffmann-Campo,Clara B.
Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) is being used in Brazil as a biological insecticide. Host plant resistance of soybean to insects is been searched for and some authors have mentioned the interference of plant chemistry in virus efficiency. Interactions among soybean extracts of genotypes used as a source of resistance (PI 274454 and PI 227687) with different AgMNPV concentrations in populations of A. geatalis susceptible (S) and resistant (R) to the virus were studied at laboratory condition. Higher mortality was observed when larvae fed on diets with extracts of the soybean genotypes compared with those fed on a plain diet (control). The mean lethal concentration (LC50) was reduced about 10 ties in the S-population fed on diets...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Velvetbean caterpillar; Baculovirus; Leaf extracts; Resistant genotypes; Soybean.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000400021

Artificial diet for laboratory rearing of Condylorrhiza vestigialis (Guenée, 1854) (Lep.: Crambidae)
Provedor de dados: 42
Autores: CAMPOS,LUCAS S.; COELHO JR,ALOISIO; PARRA,JOSÉ ROBERTO P.
ABSTRACT The Brazilian Poplar Moth, Condylorrhiza vestigialis (Guenée), compromises the wood productivity of poplar trees (Populus sp.), mainly affecting the matchstick industry in southern Brazil. Considering the lack of information on rearing techniques for this insect, the objective of this study was to develop an artificial diet to rear C. vestigialis with biological characteristics similar to the wild insects. A properly diet will enable bio-ecological studies and biological control programs using the baculovirus Condylorrhiza vestigialis multiple nucleopolyhedrovirus (CvMNPV). To attain this objective, first, three artificial diets were tested. Only the diet based on corn, wheat germ and yeast as a protein source (Diet 3) was able to supply the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IPM; Biological control; Forest entomology; Baculovirus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100333

Enhanced activity of Anticarsia gemmatalis Hüb. (Lepidoptera: Noctuidae) nuclear polyhedrosis virus by boric acid in the laboratory
Provedor de dados: 44
Autores: Morales,Lauro; Moscardi,Flávio; Sosa-Gómez,Daniel R.; Paro,Fábio E.; Soldorio,Ivanilda L.
Boric acid concentrations (0.02,0.03,0.045,0.067 and 0.101 g/100 ml of diet) were evaluated in combination with the Anticarsia gemmatalis Hüb. nuclear polyhedrosis virus (AgNPV) for enhanced virali activity against the insect. Seven days after inoculation, the median lethal concentration (LC50) was 1.52 x 10(5) for the AgNPV alone and 7.95 x 10² for the NPV mixed with 0.045g of boric acid/100 ml of diet. At subsequent evaluation dates (9,11 and 14 days after inoculation) LC50's for NPV+boric acid were ca. 4x lower than those observed for the NPV alone. The median lethal time (LT50) was 13.6 days when the NPV was used alone, while, when in mixture with increasing concentrations of boric acid, LT50 values ranged from 13.7 days (boric acid at 0.02g/100 ml of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Velvetbean caterpiliar; Biological control; Baculovirus.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000100016

Levantamento dos inimigos naturais da lagarta do cartucho do milho, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), na região de Cascavel, PR
Provedor de dados: 44
Autores: Valicente,Fernando H.; Barreto,Marliton R.
A survey of the natural enemies of S. frugiperda larvae was done during the years of 1992/93 and 94/95, in Cascavel region, Parana State. The most frequent parasitoids were Campoletis sp. (Hymenoptera: Ichneumonidae), which reached 47.0% of parasitism and Archytas marmoratus (Diptera: Tachinidae), which reached 15.4%. Of all Baculovirus found, 10.8% were NPV (nuclear polihedrosis virus) and only 1% was GV (granulosis virus).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Parasitoids; Baculovirus; Fungi.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000200018

Production of nuclear polyhedrosis virus of Anticarsia gemmatalis Hübner (Lepidoptera:Noctuidae): effect of vírus dosage, host density and age
Provedor de dados: 44
Autores: Moscardi,Flávio; Leite,Luiz G.; Zamataro,Carlos E.
The laboratory production of Anticarsia gemmatalis Hübner nuclear polyhedrosis virus (AgNPV) was evaluated for larvae inoculated at 1.0, 1.5, 2.0, and 2.5 cm and with two NPV dosages, 10(6) and 10(7) polyhedron inclusion bodies (PIB)/ml, sprayed on the insect diet surface. A fixed population of 25 larvae/300-ml cup was used. Highest virus yield was achieved for larvae inoculated with ca. 2.0 cm at the higher NPV dosage. Losses of NPV-inoculated larvae ranged from 42.4% to 64%, due to high incidence of canibalism, death by other causes, and pupation. In another experiment, larvae were inoculated with two NPV dosages (1.0 x 10(7) and 4.0 x 10(7) PIB/ml), using 25,20, and 15 larvae/cup at the beginning of 4th instar (B4L); end of 4th instar (E4L); beginning...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Velvetbean caterpillar; Baculovirus.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000100017

Application rate trials with a nuclear polyhedrosis virus to control Spodoptera frugiperda (Smith) on maize
Provedor de dados: 44
Autores: Cruz,Ivan; Figueiredo,Maria L. C.; Valicente,Fernando H.; Oliveira,António C.
Different concentrations of nuclear polyhedrosis virus were applied as aqueous suspensions using a tractor mounted or a backpack-manual sprayer to control Spodoptera frugiperda (Smith) larvae on maize (Zea mays L.). Larval mortality varied with the application equipment. For the tractor-mounted sprayer application of at least 2.5 x 10(12) P.I.B./ha was necessary to attain a control comparable to that obtained with the backpack-manual sprayer application (70.2%). For this application method it can be used the dose of 2.5 x 10(11) PI.B./ ha, butNPV persistence was very short. A greaterNPV persistence was obtained with doses over 1.25 x 10(12) P.I.B./ha (93.4% mortality). The occurrence of parasitoids in the experimental area increased larval mortality rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Fall armyworm; Baculovirus; Biological control.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000100019

Microencapsulation of a Colombian Spodoptera frugiperdaNucleopolyhedrovirus with Eudragit® S100 by spray drying
Provedor de dados: 52
Autores: Camacho Kurmen,Judith Elena; Gómez Alvarez,Martha Isabel; Villamizar Rivero,Laura Fernanda.
A Colombian Spodoptera frugiperda nucleopolyhedrovirus NPV003 with high potential for the development of an efficient biopesticide was microencapsulated by spray drying with a pH dependent polymer (Eudragit® S100). Conditions for microparticles production were standardized and microencapsulation process was validated. Physical properties, insecticide activity and photo-stability of microencapsulated virus were determined. The microparticles were spherical and irregular shaped, with sizes between 17.64 and 19.47 µm. Moisture content was 10.38 ± 0.87%; encapsulation efficiency 84.61± 13.09% and process yield was 91.20 ± 6.40%. Microencapsulation process did not affect viral insecticidal activity and provided efficient protection against UVB radiation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; Microencapsulation; Spray drying; Eudragit® S100; Photostability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300468

Characterization of a Chrysodeixis includens nucleopolyhedrovirus Isolate from Brazilian Cerrado and Assessment of its Co-Infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus
Provedor de dados: 52
Autores: Sanches,Márcio Martinello; Sihler,William; Silva,Cláudia Efigênia Pereira; Guimarães,Giovana Curcio; Benito,Norton Polo; Sosa-Gómez,Daniel Ricardo; de Souza,Marlinda Lobo.
Abstract Chrysodeixis includens has become the major Lepidopteran pest of soybean crops, especially in the Brazilian Cerrado (savanna) region. A native isolate of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) from this region, Buritis, MG, was assessed for its biological and molecular features. In addition, in vitro co-infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), another virus of an important soybean pest, was tested. The ChinNPV-Buritis isolate presented an average LC50 of 7,750 occlusion bodies (OBs)/ml of diet in C. includens larvae. Analysis of restriction endonuclease profiles of viral DNA revealed similarities with previously described ChinNPV isolates IE, IF, and IG from Brazil, although the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgMNPV; Baculovirus; ChinNPV; Soybean looper; Velvetbean caterpillar.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100218

Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
Provedor de dados: 52
Autores: Almeida,Andréa Farias de; Macedo,Gorete Ribeiro de; Chan,Leslie Chee Loong; Pedrini,Márcia Regina da Silva.
In this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean rate of infection, as well as the mean rate of occlusion body production were determined during the infection of these cell-lines with the extracellular virus of the S. frugiperda nucleopolyhedrovirus (SfMNPV). The Sf9 cell-line resulted in better viral production results (5.0 x 10(8) OB/mL) than the Sf21 cell-line (2.5 x 10(8) OB/mL).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; In vitro production; Kinetic analysis; SfMNPV.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200006

Expression of the Mycobacterium bovis P36 gene in Mycobacterium smegmatis and the baculovirus/insect cell system
Provedor de dados: 56
Autores: Bigi,F.; Taboga,O.; Romano,M.I.; Alito,A.; Fisanotti,J.C.; Cataldi,A.A.
In the present study we evaluated different systems for the expression of mycobacterial antigen P36 secreted by Mycobacterium bovis. P36 was detected by Western blot using a specific antiserum. The P36 gene was initially expressed in E. coli, under the control of the T7 promoter, but severe proteolysis prevented its purification. We then tried to express P36 in M. smegmatis and insect cells. For M. smegmatis, we used three different plasmid vectors differing in copy number and in the presence of a promoter for expression of heterologous proteins. P36 was detected in the cell extract and culture supernatant in both expression systems and was recognized by sera from M. bovis-infected cattle. To compare the expression level and compartmentalization, the MPB70...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Mycobacterium smegmatis; Insect cells; Baculovirus; Secreted proteins; P36; MPB70; Antigen.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100004

Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain
Provedor de dados: 56
Autores: Miranda,E.A.; de-Murcia,G.; Ménissier-de-Murcia,J.
A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 109 infected cells in three liters of culture
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PARP; Insect cells; Baculovirus; Overproduction.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800002

Construction of occluded recombinant baculoviruses containing the full-length cry1Ab and cry1Ac genes from Bacillus thuringiensis
Provedor de dados: 56
Autores: Ribeiro,B.M.; Crook,N.E.
The administration of baculoviruses to insects for bioassay purposes is carried out, in most cases, by contamination of food surfaces with a known amount of occlusion bodies (OBs). Since per os infection is the natural route of infection, occluded recombinant viruses containing crystal protein genes (cry1Ab and cry1Ac) from Bacillus thuringiensis were constructed for comparison with the baculovirus prototype Autographa californica nucleopolyhedrovirus (AcNPV). The transfer vector pAcUW2B was used for construction of occluded recombinant viruses. The transfer vector containing the crystal protein genes was cotransfected with linearized DNA from a non-occluded recombinant virus. The isolation of recombinant viruses was greatly facilitated by the reduction of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; AcNPV; Bacillus thuringiensis cry1A; Heliothis virescens; Pieris brassicae.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600006