Consulta: subjectFacets:"Beta-lactamase"
Registros recuperados: 6
Data/hora: 11/06/2024 13:18:32

Concentração mínima inibitória de dez antimicrobianos para amostras de Staphylococcus aureus isoladas de infecção intramamária bovina
Provedor de dados: 45
Autores: Brito,M.A.V.P.; Brito,J.R.F.; Silva,M.A.S.; Carmo,R.A.
Determinou-se a concentração mínima inibitória (CMI) de ampicilina, cefalotina, eritromicina, gentamicina, neomicina, norfloxacina, oxacilina, penicilina G, tetraciclina e tilosina para 112 amostras de Staphylococcus aureus isoladas de infecções intramamárias bovina, em 33 rebanhos leiteiros da Zona da Mata do Estado de Minas Gerais. Foram isoladas 24 amostras de infecção clínica, 66 de subclínica e 22 de infecção crônica. As amostras de infecção crônica foram isoladas repetidas vezes dos mesmos quartos mamários de nove vacas de um rebanho, no período de 13 meses. A CMI foi realizada em ágar Mueller Hinton, com concentrações entre 0,015 e 128µgml-1 de cada antimicrobiano. As amostras da American Type Culture Collection (ATCC) recomendadas para controle de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastite bovina; Staphylococcus aureus; Susceptibilidade antimicrobiana; Concentração mínima inibitória; Beta-lactamase.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500003

beta-lactamase production Haemophilus spp. and resistance to ampicillin in a general hospital in Porto Alegre city, RS, Brazil (2001-2005)
Provedor de dados: 55
Autores: Ferreira,Jorge A. S.; Castro,Andréa Cauduro de; Rocha,Marion P.; Riboldi,Gustavo; d'Azevedo,Pedro Alves.
In a four-year period (July/2001-June/2005), 410 Haemophilus spp. isolates were studied. Those were isolated from sputum at Hospital Nossa Senhora da Conceição (NSC) in Porto Alegre city (RS). beta-lactamase enzyme was detected in 113 (27.6%) of isolates through chromogenic cephalosporin method. Fifty-eight (51.3%) of them showed sensibility to ampicillin through disc-diffusion method using Haemophilus Test Medium (HTM) by NCCLS criteria. In 297 (72.4%) isolates beta-lactamase was not detected by chromogenic cephalosporin method. Five (1.7%) of them were resistant and 1 (0.3%) intermediate to ampicillin using disc-diffusion method. The authors emphasized the importance of Haemophilus spp. resistance to ampicillin research in clinical laboratories routine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haemophilus spp.; Beta-lactamase; Antimicrobial resistance; Ampicillin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100013

Conventional versus molecular tests (Multiplex PCR and PCR mecA gene) for detection of methicillin resistant Staphylococcus aureus
Provedor de dados: 58
Autores: Ribas,Rosineide Marques; Gontijo-Filho,Paulo Pinto; Darini,Ana Lúcia da Costa.
In this study, for detection of methicillin-resistant S. aureus (MRSA), a mecA multiplex PCR-based amplification was compared with the 1 µg oxacillin disk diffusion test, detection of minimal inhibitory concentration (MIC), and screening in agar with 4% NaCl and 6 µg/mL oxacillin. Among 24 isolates obtained from blood, mecA gene was detected in only 16 (66.7%) isolates by multiplex PCR. The MIC test showed a range of resistance to oxacillin from 0.19 to 512 µg/mL, among these isolates. Data obtained by screening and dilution tests showed that sensitivity to methicillin was 80.0% and 72.8%, respectively, when compared with the presence of mecA gene (multiplex). All isolates, including the negatives, when revaluated for mecA gene by PCR were positive....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiplex PCR; MRSA; Beta-lactamase; MecA gene.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500012

Extended-spectrum beta-lactamase production among ampicillin-resistant Escherichia coli strains from chicken in Enugu State, Nigeria
Provedor de dados: 58
Autores: Chah,K.F.; Oboegbulem,S.I.
One hundred and seventy-two ampicillin-resistant E. coli strains isolated from commercial chickens in Enugu State, Nigeria, were screened for beta-lactamase production using the broth method with nitrocefin® as the chromogenic cephalosporin to detect enzyme production. Beta-lactamase producing strains were further examined for extended-spectrum beta-lactamase (ESBL) production using the Oxoid combination discs method. One hundred and seventy (98.8%) of the 172 ampicillin-resistant E. coli strains produced beta-lactamase enzyme. Sixteen (9.4%) beta-lactamase producers were phenotypically confirmed to produce ESBLs. Six of the ESBL producing strains were only detected with ceftazidime versus ceftazidime/clavulanate combination while ten of the ESBL producers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ampicillin-resistant; Escherichia coli; Beta-lactamase; Extended-spectrum.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400018

Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
Provedor de dados: 58
Autores: Alam,Mohammad Zubair; Aqil,Farrukh; Ahmad,Iqbal; Ahmad,Shamim.
This study reports the occurrence of antibiotic resistance and production of β-lactamases including extended spectrum beta-lactamases (ESβL) in enteric bacteria isolated from hospital wastewater. Among sixty-nine isolates, tested for antibiotic sensitivity, 73.9% strains were resistant to ampicillin followed by nalidixic acid (72.5%), penicillin (63.8%), co-trimoxazole (55.1%), norfloxacin (53.6%), methicillin (52.7%), cefuroxime (39.1%), cefotaxime (23.2%) and cefixime (20.3%). Resistance to streptomycin, chloramphenicol, nitrofurantoin, tetracycline, and doxycycline was recorded in less than 13% of the strains. The minimum inhibitory concentration (MIC) showed a high level of resistance (800-1600 µg/mL) to one or more antibiotics. Sixty three (91%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta-lactamase; ESβL; Antibiotic resistance; Enteric bacteria; Plasmid transfer.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300021

Correlation of phenotypic tests with the presence of the blaZ gene for detection of beta-lactamase
Provedor de dados: 58
Autores: Ferreira,Adriano Martison; Martins,Katheryne Benini; Silva,Vanessa Rocha da; Mondelli,Alessandro Lia; Cunha,Maria de Lourdes Ribeiro de Souza da.
Abstract Staphylococcus aureus and Staphylococcus saprophyticus are the most common and most important staphylococcal species associated with urinary tract infections. The objective of the present study was to compare and to evaluate the accuracy of four phenotypic methods for the detection of beta-lactamase production in Staphylococcus spp. Seventy-three strains produced a halo with a diameter ≤28 mm (penicillin resistant) and all of them were positive for the blaZ gene. Among the 28 susceptible strain (halo ≥29 mm), 23 carried the blaZ gene and five did not. The zone edge test was the most sensitive (90.3%), followed by MIC determination (85.5%), but the specificity of the former was low (40.0%). The nitrocefin test was the least sensitive (28.9%)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary tract infection; Staphylococcus saprophyticus; Zone edge test; Beta-lactamase; BlaZ.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100159