Consulta: subjectFacets:"Biological activity"
Registros recuperados: 19
Data/hora: 05/06/2024 18:29:59

Incidencia del gusano cogollero Spodoptera frugiperda Smit en Ocoyucan, Puebla y actividad bioinsecticida de semillas de Carica papaya L. y Trichilia havanensis Jacq.
Provedor de dados: 32
Autores: Figueroa Brito, Rodolfo.
El maíz es el principal cultivo de México y constituye la base de la alimentación de la población. Entre los factores que limitan la producción en este cultivo están los insectos plaga, destacando los daños ocasionados por el gusano cogollero Spodoptera frugiperda. Se evaluaron en laboratorio los productos naturales de las semillas de Carica papaya y Trichilia havanensis sobre S. frugiperda y su enemigo natural Chrysoperla carnea. En invernadero, se evaluó el sulfato de amonio (13.6 g), urea (6 g), fosfonitrato (6.56 g) y la vermicomposta con tierra negra (1:3) en bolsas de plástico (6 kg) junto con el extracto acuoso de semillas de C. papaya al 20 % en plantas de maíz. En campo, se entrevistaron 35 productores de maíz del municipio de Ocoyucan, Puebla,...
Palavras-chave: Daño-maíz; Manejo tradicional; Caricaceae; Meliaceae; Productos naturales; Actividad biológica; Efectos secundarios; Chrysoperla carnea; Damage-corn; Traditional management; Natural products; Biological activity; Secondary effects; Doctorado; EDAR; Estrategias para el Desarrollo Agrícola Regional.
Ano: 2011
URL: http://hdl.handle.net/10521/494

Incidencia del gusano cogollero Spodoptera frugiperda Smit en Ocoyucan, Puebla y actividad bioinsecticida de semillas de Carica papaya L. y Trichilia havanensis Jacq.
Provedor de dados: 32
Autores: Figueroa Brito, Rodolfo.
El maíz es el principal cultivo de México y constituye la base de la alimentación de la población. Entre los factores que limitan la producción en este cultivo están los insectos plaga, destacando los daños ocasionados por el gusano cogollero Spodoptera frugiperda. Se evaluaron en laboratorio los productos naturales de las semillas de Carica papaya y Trichilia havanensis sobre S. frugiperda y su enemigo natural Chrysoperla carnea. En invernadero, se evaluó el sulfato de amonio (13.6 g), urea (6 g), fosfonitrato (6.56 g) y la vermicomposta con tierra negra (1:3) en bolsas de plástico (6 kg) junto con el extracto acuoso de semillas de C. papaya al 20 % en plantas de maíz. En campo, se entrevistaron 35 productores de maíz del municipio de Ocoyucan, Puebla,...
Palavras-chave: Daño-maíz; Manejo tradicional; Caricaceae; Meliaceae; Productos naturales; Actividad biológica; Efectos secundarios; Chrysoperla carnea; Damage-corn; Traditional management; Natural products; Biological activity; Secondary effects; Doctorado; EDAR; Estrategias para el Desarrollo Agrícola Regional.
Ano: 2011
URL: http://hdl.handle.net/10521/494

Production, purification and characterization of recombinant human antithrombin III by Saccharomyces cerevisiae
Provedor de dados: 69
Autores: Mallu,Maheswara Reddy; Vemula,Sandeep; Ronda,Srinivasa Reddy.
Background: Antithrombin III (ATIII) is a protein that inhibits abnormal blood clots (or coagulation) by breaking down thrombin and factor Xa. ATIII helps to keep a healthy balance between hemorrhage and coagulation. The present work demonstrated the production, purification and characterization of recombinant human antithrombin (rhAT) from yeast Saccharomyces cerevisiae BY4741 was demonstrated. After expression of rhAT by S. cerevisiae, the biomass and rhAT concentration were analyzed through fed-batch fermentation process. Results: In fed-batch fermentation, the biomass (maximum cell dry weight of 11.2 g/L) and rhAT concentration (312 mg/L) of the expressed rhAT were achieved at 84 h of cultivation time. The maximum cell lysis efficiency (99.89%) was...
Tipo: Journal article
Palavras-chave: Biological activity; Cell lysis; Cross flow filtration; Fed-batch fermentation; Purification; Secondary structure.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400012

BIOPROSPECTING: A TOOL TO CONSERVE CHILEAN BRYOPHYTES
Provedor de dados: 73
Autores: Cuvertino-Santoni,Jorge; Montenegro,Gloria.
Here, we present the current state of knowledge on the diversity of Chilean bryophytes (mosses, liverworts, and hornworts), the secondary metabolites present in these plants, and the biological activity of extracts from populations of Chilean species. Our goal is to establish the potential of these plants as a source of useful molecules for pharmaceutical and agricultural industries, thus promoting their conservation. Only 3,7% of Chilean bryophytes (55 spp.) have been analyzed using chemical characterization of their extracts (methanol, ether, ethanol, dichloromethane, acetone and hexane). Only four species from Chilean and Argentinian populations (Porella chilensis (Lehm. &amp;amp; Lindenb.) Trevi., Riccardiapolyclada (Mitt. ex Thurn) Hässel,...
Tipo: Journal article
Palavras-chave: Mosses; Liverworts; Hornworts; Uses; Secondary metabolites; Biological activity; In vitro cultivation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432013000100002

Marine Polysaccharides: A Source of Bioactive Molecules for Cell Therapy and Tissue Engineering
Provedor de dados: 5
Autores: Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia.
The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the...
Tipo: Text
Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf

Accounting for Rough Bed Friction Factors of Mud Beds as a Result of Biological Activity in Erosion Experiments
Provedor de dados: 5
Autores: Guizien, Katell; Orvain, Francis; Duchene, Jean-claude; Le Hir, Pierre.
The average bed shear stress and bed friction factor of samples with any roughness was derived from the head loss between upstream and downstream of a test section in an erosion tunnel. The method was validated in both hydraulically smooth (plexiglass; Reynolds number less than 25,000) and rough regimes (calibrated particles with known roughness). As a first step toward using this method on natural sediment, this method was tested with experimental mesocosms assembled from field collected materials (sieved sediments; diatoms). Bed shear stress measurement precision was high enough in the experiments to detect a positive significant relationship between bed friction factor and core roughness. The observed bed friction factor increase could be related to...
Tipo: Text
Palavras-chave: Bed roughness; Friction factor; Mud beds; Biological activity; Erosion experiments.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00117/22807/20617.pdf

Antimicrobial Compounds from Eukaryotic Microalgae against Human Pathogens and Diseases in Aquaculture
Provedor de dados: 5
Autores: Falaise, Charlotte; Francois, Cyrille; Travers, Marie-agnes; Morga, Benjamin; Haure, Joel; Tremblay, Rejean; Turcotte, Francois; Pasetto, Pamela; Gastineau, Romain; Hardivillier, Yann; Leignel, Vincent; Mouget, Jean-luc.
The search for novel compounds of marine origin has increased in the last decades for their application in various areas such as pharmaceutical, human or animal nutrition, cosmetics or bioenergy. In this context of blue technology development, microalgae are of particular interest due to their immense biodiversity and their relatively simple growth needs. In this review, we discuss about the promising use of microalgae and microalgal compounds as sources of natural antibiotics against human pathogens but also about their potential to limit microbial infections in aquaculture. An alternative to conventional antibiotics is needed as the microbial resistance to these drugs is increasing in humans and animals. Furthermore, using natural antibiotics for...
Tipo: Text
Palavras-chave: Biological activity; Antimicrobial; Antibacterial; Antifungal; Antiviral; Biological activity; Haslea; Microalgae; Sustainable aquaculture.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00349/46052/45719.pdf

NO-LINEAR RESPIRATION DYNAMICS IN A DEGRADED ALFISOL AMENDED WITH DIFFERENT DOSE OF SALMON SLUDGES
Provedor de dados: 126
Autores: Celis,J.E; Sandoval,M.A; Bello,N.
No research has been conducted regarding respiration model parameters as a consequence of the application of salmon sludge on degraded Alfisols. In order to investigate kinetic parameters representing the decomposition of salmon sludge by means of respiration rates, sediment samples from a land-farmed pisciculture and a lake-salmon facility were applied to a degraded Alfisol at the Biobio region (Chile). Soil microbiological activity was evaluated on a 60-days laboratory incubation experiment under different salmon sludge addition dose: T1 25 t ha-1; T2: 50 t ha-1; T3: 75 t ha-1; T4: 100 t ha-1; T5: 150 t ha-1. A non-amended soil served as control. C0(2)-C production was determined in samples under closed incubation. A two-component first-order regression...
Tipo: Journal article
Palavras-chave: Aquaculture; Decomposition of organic matter; K-value; Biosolids; Soil remediation; Biological activity.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162011000100005

SOIL QUALITY IN RELATION TO FOREST CONVERSION TO PERENNIAL OR ANNUAL CROPPING IN SOUTHERN BRAZIL
Provedor de dados: 90
Autores: Balota,Elcio Liborio; Yada,Ines Fumiko Ubukata; Amaral,Higo Furlan; Nakatani,Andre Shigueyoshi; Hungria,Mariangela; Dick,Richard Peter; Coyne,Mark Steven.
Many forested areas have been converted to intensive agricultural use to satisfy food, fiber, and forage production for a growing world population. There is great interest in evaluating forest conversion to cultivated land because this conversion adversely affects several soil properties. We examined soil microbial, physical, and chemical properties in an Oxisol (Latossolo Vermelho distrófico) of southern Brazil 24 years after forest conversion to a perennial crop with coffee or annual grain crops (maize and soybeans) in conventional tillage or no-tillage. One goal was to determine which soil quality parameters seemed most sensitive to change. A second goal was to test the hypothesis that no-tillage optimized preservation of soil quality indicators in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Land use; Tillage; Coffee; Biological activity; Potential mineralization; Aggregate stability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000401003

Biflorin: an o-naphthoquinone of clinical significance
Provedor de dados: 42
Autores: WISINTAINER,GABRIELLE G.N.S.; SIMÕES,EVELYNE R.B.; LEMOS,TELMA L.G.; MOURA,SIDNEI; SOUZA,LUCIANA G.S.; FONSECA,ALUISIO M.; MORAES,MANOEL ODORICO; PESSOA,CLAUDIA; ROESCH-ELY,MARIANA; HENRIQUES,JOÃO A.P.
Biflorin is an o-naphthoquinone with proven cytotoxic effects on tumor cells showing antimicrobial, antitumor and antimutagenic activities. Biflorin is an isolated compound taken from the roots of the plantCapraria biflora L. (Schrophulariaceae), indigenous of the West Indies and South America, which is located in temperate or tropical areas. This compound has shown to be strongly active against grampositive and alcohol-acid-resistant bacteria. It has been efficient in inhibiting the proliferation tumor cell lines CEM, HL-60, B16, HCT-8 and MCF-7. Recently, SK-Br3 cell line was treated with biflorin showing important cytotoxic effects. In this article, information related to the first structural characterization studies are presented, as well as the latest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological activity; Biflorin; Capraria biflora; Natural products.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401907

Antioxidant, hemolytic, antimicrobial, and cytotoxic activities of the tropical Atlantic marine zoanthid Palythoa caribaeorum
Provedor de dados: 42
Autores: ALENCAR,DANIEL B.; MELO,ARTHUR A.; SILVA,GISELLE C.; LIMA,REBECA L.; PIRES-CAVALCANTE,KELMA M.S.; CARNEIRO,RÔMULO F.; RABELO,ADRIANA S.; SOUSA,OSCARINA V.; VIEIRA,REGINE H.S.F.; VIANA,FRANCISCO A.; SAMPAIO,ALEXANDRE H.; SAKER-SAMPAIO,SILVANA.
Marine invertebrates are capable of synthesizing bioactive compounds, which may be beneficial to human health. The aim of this study was to evaluate the antioxidant, hemolytic, antimicrobial and cytotoxic activities of crude extract (70% EtOH), and dichloromethane (DCM), ethyl acetate (EtOAc), and aqueous (Aq) fractions of the marine zoanthid Palythoa caribaeorum. The phenolic compound contents of the crude extract, DCM, EtOAc and Aq fractions were 12.33, 18.17, 10.53, and 3.18 mg GAE per gram, respectively. DPPH radical scavenging activity showed slight variation. IC50 of crude extract, DCM, EtOAc and Aq fractions were 11.13, 11.25, 11.74, and 11.28 µg mL-1, respectively. Among the sample, ferrous ion chelating was the highest in crude extract (IC50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological activity; Marine invertebrate; Natural product; Sphenopidae.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201113

Construction and application of a yeast expression system for thymosin a1
Provedor de dados: 47
Autores: Chen,Feng; Chen,Xiang-Ming; Chen,Zhi; Jiang,Han-Liang; Pan,Xiao-Ping; Hu,Zhong-Rong; Liu,Rong-Hua; Chen,Xiao-Ming.
We want to construct a yeast expression system for thymosin a1 (Ta1) to make the orally administered Ta1 preparation possible. The whole Ta1 DNA fragment was obtained by PCR. After being digested with restriction enzymes, it was cloned into pYES2 vector. Sequencing was performed to identify the recombinant. The sequence of Ta1 in recombinant coincided with the original one reported in Genbank. When pYES2-Ta1 plasmid was transformed into yeast, galactose instead of glucose was used to induce Ta1 expression. Western blot was performed to identify the quality of the expressed Ta1. Dried yeast containing pYEST2-Ta1 was fed to Balb/c mice whose immunities were inhibited by cyclophosphamide in advance. Synthesized Ta1 peptide was used as positive control and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thymosin a1; Yeast expression system; Biological activity; Orally administered.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300002

ENSAYOS DE ACTIVIDAD BIOLÓGICA Y ECOLOGÍA QUÍMICA DE EXTRACTOS ORGÁNICOS DE MACROALGAS DEL CARIBE COLOMBIANO
Provedor de dados: 50
Autores: Díaz R.,Martha Cecilia; Bula Meyer,German; Zea,Sven; Martínez,Alejandro.
This study evaluates the biological activity and possible deterrance played by organic crude extracts from six Phaeophyta and two Rhodophyta algae from Southern Caribbean. Bioessays were carried out using concentrations equivalent to those present in algae tissue. Species in general did not show high citotoxic activity. However Dictyota pulchella, showed a delay in the mitotic process for cells stage XVI. Extracts from Dictyota bartayresiana, D. pulchella, Sargassum cymosum and Cladophyllum schnetteri, inhibited S. partitus feeding under laboratory conditions. Nontheless, only extracts from D. bartayresiana and C. schnetteri showed this activity in the field.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Macroalgae; Colombian Caribbean; Chemical ecology; Biological activity.
Ano: 2006
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612006000100015

Cocos nucifera (L.) (Arecaceae): A phytochemical and pharmacological review
Provedor de dados: 56
Autores: Lima,E.B.C.; Sousa,C.N.S.; Meneses,L.N.; Ximenes,N.C.; Santos Júnior,M.A.; Vasconcelos,G.S.; Lima,N.B.C.; Patrocínio,M.C.A.; Macedo,D.; Vasconcelos,S.M.M.
Cocos nucifera (L.) (Arecaceae) is commonly called the “coconut tree” and is the most naturally widespread fruit plant on Earth. Throughout history, humans have used medicinal plants therapeutically, and minerals, plants, and animals have traditionally been the main sources of drugs. The constituents of C. nucifera have some biological effects, such as antihelminthic, anti-inflammatory, antinociceptive, antioxidant, antifungal, antimicrobial, and antitumor activities. Our objective in the present study was to review the phytochemical profile, pharmacological activities, and toxicology of C. nucifera to guide future preclinical and clinical studies using this plant. This systematic review consisted of searches performed using scientific databases such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucifera (L.); Ethnopharmacology; Pharmacological properties; Biological activity; Review.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100953

Biological activity of Serratia marcescens cytotoxin
Provedor de dados: 56
Autores: Carbonell,G.V.; Amorim,C.R.N.; Furumura,M.T.; Darini,A.L.C.; Fonseca,B.A.L.; Yano,T.
Serratia marcescens cytotoxin was purified to homogeneity by ion-exchange chromatography on a DEAE Sepharose Fast Flow column, followed by gel filtration chromatography on a Sephadex G100 column. The molecular mass of the cytotoxin was estimated to be about 50 kDa. Some biological properties of the cytotoxin were analyzed and compared with well-characterized toxins, such as VT1, VT2 and CNF from Escherichia coli and hemolysin produced by S. marcescens. The sensitivity of the cell lines CHO, HeLa, HEp-2, Vero, BHK-21, MA 104 and J774 to the cytotoxin was determined by the cell viability assay using neutral red. CHO and HEp-2 were highly sensitive, with massive cellular death after 1 h of treatment, followed by BHK-21, HeLa, Vero and J774 cells, while MA 104...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell culture; Biological activity; Serratia marcescens; Cytotoxin; Virulence factors.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300010

Expression, production, and renaturation of a functional single-chain variable antibody fragment (scFv) against human ICAM-1
Provedor de dados: 56
Autores: Sun,H.; Wu,G.M.; Chen,Y.Y.; Tian,Y.; Yue,Y.H.; Zhang,G.L.
Intercellular adhesion molecule-1 (ICAM-1) is an important factor in the progression of inflammatory responses in vivo. To develop a new anti-inflammatory drug to block the biological activity of ICAM-1, we produced a monoclonal antibody (Ka=4.19×10−8 M) against human ICAM-1. The anti-ICAM-1 single-chain variable antibody fragment (scFv) was expressed at a high level as inclusion bodies in Escherichia coli. We refolded the scFv (Ka=2.35×10−7 M) by ion-exchange chromatography, dialysis, and dilution. The results showed that column chromatography refolding by high-performance Q Sepharose had remarkable advantages over conventional dilution and dialysis methods. Furthermore, the anti-ICAM-1 scFv yield of about 60 mg/L was higher with this method. The purity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intercellular adhesion molecule-1; Single-chain variable antibody fragment; Expression; Purification; Renaturation; Biological activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700540

Chemical composition, antioxidant and biological activity of Ocotea bicolor Vattimo-Gil (LAURACEAE) essential oil
Provedor de dados: 60
Autores: Damasceno,Carolina Sette Barbosa; Oliveira,Letícia Freire de; Szabo,Ellis Marina; Souza,Ângela Maria; Dias,Josiane Fatima Gaspari; Miguel,Marilis Dalarmi; Miguel,Obdúlio Gomes.
ABSTRACT The essential oil composition of the Ocotea bicolor, native plant of Brazil, was studied for the first time. The essential oil of the leaf was obtained by hydrodistillation and analyzed by GC/MS. The analytical procedure revealed a predominance of sesquiterpenes, δ-cadinene (7.39%), β-sesquiphellandrene (6.67%), β-elemene (5.41%) and α-cadinol (5,23%). The essential oil was submitted to brine shrimp toxicity evaluation, antioxidant and antibacterial tests. The antioxidant activity by the formation of phosphomolybdenum complex method presented positive results. The minimum inhibitory concentration (MIC) values were higher than 1000 μg/mL for the microorganisms tested. Toxicity activity revealed LC50 results of 40.10 (μg/mL), being toxic to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocotea bicolor/Oil essential/chemical composition; Lauraceae; Antioxidant activity; Biological activity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400615

Effect of organic and mineral amendments upon soil respiration and microbial biomass in a saline-sodic soil
Provedor de dados: 118
Autores: Celis,José E; Sandoval,Marco; Martínez,Bárbara; Quezada,Celerino.
J.E. Celis, M. Sandoval, B. Martínez, and C. Quezada. 2013. Effect of organic and mineral amendments upon soil respiration and microbial biomass in a saline-sodic soil. Cien. Inv. Agr. 40(3): 571-580. An understanding of soil carbon stocks and fluxes in saline-sodic soils is becoming critical in environmental management because salinity and sodicity are predicted to increase worldwide. The effects of amendment with sewage sludge (SW), mined gypsum (MG) and synthetic gypsum (SG) on the soil respiration rate and soil (S) microbial biomass (SMB) of a saline-sodic soil were assessed in vitro over 60 days under controlled conditions. The treatments were: T1 = S + MG (7 t ha-1); T2 = S + MG (7 t ha-1) + SW (90 t ha-1); T3 = S + SG (7 t ha-1); T4 = S + SG (7 t...
Tipo: Journal article
Palavras-chave: Arid soil; Biological activity; Gypsum; Organic matter; Sewage sludge; Soil respiration.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300010

Yield, yield features, phytochemical composition, antioxidant and antibacterial activities of Abutilon indicum cultivated under different fertilizers
Provedor de dados: 118
Autores: Yaldiz,Gulsum; Yildirim,Arzu Birinci; Arici,Yeliz Kasko; Camlica,Mahmut.
G. Yaldiz, A. B. Yildirim, Y. K. Arici, and M. Camlica. 2016. Yield, yield features, phytochemical composition, antioxidant and antibacterial activities of Abutilon indicum cultivated under different fertilizers. Cien. Inv. Agr. 43(3):464-475. Abutilon indicum (Link) Sweet is an important medicinal plant that has been used in traditional medicine for centuries, and it is one ofthe novel crops being used in the pharmaceutical industry. Agricultural practices that produce high drug yield and desired secondary metabolites are in high demand by pharmaceutical-related industries; therefore, the aim of this study was to determine the leaf and seed yield, crude oil yield, crude oil composition, total phenolic content, and the antioxidant and antibacterial...
Tipo: Journal article
Palavras-chave: Biological activity; CAN; Crude oil; Fertilizer; NPK; Yield; Aceite crudo; Actividad biológica; CAN; Fertilizante; NPK; Rendimiento.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300012