Consulta: subjectFacets:"Biological nitrogen fixation"
Registros recuperados: 68
Data/hora: 02/06/2024 03:56:03

II Curso sobre a biologia do solo na agricultura.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: ASSIS, R. L. de; SOUTO, S. M.; DUQUE, F. F.; ALMEIDA, D. L. de; MUELLER, K. E. K.
Nitogênio. Fixação de nitrogênio atmosférico na simbiose Rhizobium- leguminosas. Nodulação das leguminosas. Fatores do solo. Fatores climáticos. Fixação de nitrogênio em gramíneas. Microrganismos do solo e aproveitamento de fósforo pelas plantas. O papel das micorrizas. Outos microrganismos. Matéria orgânica do solo: Dinâmica e importância na conservação do solo e produtividade agrícola. Classificação das substâncias no solo. Microorganismos e transformação da matéria orgânica no solo. A biomassa microbiana do solo. Adição de matéria orgânica ao solo: Adubação orgânica/verde. Sistemas agrícolas adequadas a utilização da fixação biológicas no nitrogênio. Rotação planejada de culturas. Consorciação de culturas. Cultivo em faixas. Sistemas agrosilvopastorais....
Tipo: Documentos (INFOTECA-E)
Palavras-chave: P; Fixação biológica de nitrogênio; FBN; Biological nitrogen fixation; BNF.; Absorção; Adubação Verde; Biologia do Solo; Cana de Açúcar; Fertilidade do Solo; Fósforo; Gramínea; Gesso; Leguminosa Forrageira; Micorriza Vesicular Arbuscular; Palha.; Absorption; Gypsum; Grasses; Forage legumes; Green manures; Phosphorus; Soil biology; Soil fertility; Sugarcane; Vesicular arbuscular mycorrhizae.
Ano: 1992
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/623722

Fixação biológica de nitrogênio.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: ALCANTARA, R. M. C. M. de; XAVIER, G. R.; ZILLI, J. E.; MARTINS, L. M. V.; RUMJANEK, N. G.; FERNANDES JUNIOR, P. I.; LEITE, J.; SILVA JÚNIOR, E. B. da; SANTOS, C. E. de R. e S.
Fixação biológica de nitrogênio é um processo natural que ocorre pela associação simbiótica entre plantas e microrganismos do solo. Esses microrganismos são bactérias chamadas de diazotróficas, que capturam o nitrogênio do ar e o transformam em formas assimiláveis pelas plantas.
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Feijão-caupi; Biological nitrogen fixation; Cowpea; Feijão; Fixação de Nitrogênio; Nodulação; Rhizobium; Inoculação.
Ano: 2017
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1085761

Balanço de N em rotação de culturas sob plantio direto em Dourados, MS.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: ALVES, B. J. R.; ZOTARELLI, L.; ARAÚJO, E. S.; FERNANDES, F. M.; HECKLER, J. C.; MEDEIROS, A. F. A.; BODDEY, R. M.; URQUIAGA, S.
Matéria seca e N total. Quantificação da FBN eficiência do uso de N fertilizante. Balanço de N para as culturas. Resultados e discussão. Quantificação da FBN para a cultura da soja. Eficiência da fertilização. Balança de N para as culturas. O objetivo do presente estudo foi avaliar o impacto da fixação biológica de nitrogênio (FBN) na cultura da soja e a eficiência do uso de fertilizantes nitrogenados pelas culturas do milho e algodão sobre o balanço de N dos solo. O experimento foi realizando em Latossolo Vermelho distroférrico, na área experimental da Embrapa agropecuária oeste, em dourados, MS. Todas as culturas foram conduzidas em uma área onde se iniciou o plantio direto. No primeiro ano , as culturas do milho e algodão receberam 115kg N há-1 ,...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Balanço de nitrogênio; Fixação biológica de nitrogênio; FBN; Zero tillage; Direct sowing; Rotational cropping; Biological nitrogen fixation; BNF; Soybean; Maize.; Algodão; Milho; Plantio Direto; Rotação de Cultura; Soja.; Cotton; Nitrogen balance.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/626863

Microrganismos de processos biológicos do solo: perspectiva ambiental.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: SIQUEIRA, J. O.; MOREIRA, F. M. de S.; GRISI, B. M.; HUNGRIA, M.; ARAUJO, R. S.
Organismos e processos biologicos do solo; Ciclagem dos elementos no solo; Aspectos biologicos da degradacao do solo; Reabilitacao de solos degradados.
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Microorganismos; Toxicidade do aluminio; Fixacao biologica do nitrogenio; Degradacao do solo; Brasil; Microorganism; Environmental; Biological nitrogen fixation; Mycorhiza.; Acidez; Ecologia; Matéria Orgânica; Micorriza; Microbiologia; Meio Ambiente; Solo.; Acidity; Degradation; Ecology; Brazil; Organic matter; Microbiology; Soil; Toxicity.
Ano: 1994
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/449290

Quantificação da fixação biológica de nitrogênio na cultura de cana-de-açúcar utilizando a técnica de balanço de nitrogênio total no sistema solo planta.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: XAVIER, R. P.; QUESADA, D. M.; RESENDE, A. S. de; BODDEY, R. M.; ALVES, B. J. R.; URQUIAGA, S.
Fixação biológica de nitrogênio na cultura de cana-de-açúcar. Balanço de N-total do sistema solo-planta. FBN pelo balanço de N-total do sistema solo-planta.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Fixação biológica de nitrogênio; FBN; Sugar cane; Biological nitrogen fixation; BNF.; Cana de Açúcar; Saccharum Officinarum.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/627836

Uma nova estratégia para isolar Gluconacetobacter diazotrophicus de plantas de cana-de-açúcar.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: REIS, V. M.; PERIN, L.; REIS JÚNIOR, F. B. dos.
bitstream/CNPAB-2010/27164/1/cot035.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Fixação biológica de nitrogênio; FBN; Balanço de nutriente; Bactéria diazotrófica; Glunonacetobacter diazotrophicus; Biological nitrogen fixation; BNF; Diazotrophy bacteria.; Cana de Açúcar.; Nutrition physiology; Nutrient balance; Sugarcane.
Ano: 1999
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/623744

Micorrizas vesicular-arbusculares em plantas forrageiras - Aspectos agronômicos e interações microbiológicas.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: SOUTO, S. M.; PAULA, M. A. de; FRANCO, A. A.
Importância e limitações das MVA.Infecção de forrageiras por fungos micorrizicos vesicular-arbusculares (FMVA). Seleção de MVA para forrageiras.Simbiose leguminosa + FMVA + Rhizobium. Efeito de agrotóxicos na simbiose FMVA+Rhizobium+ leguminosa.Efeito de FMVA na nodulação e FBN em leguminosas. Competição da tríplice simbiose por fotossintatos.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Fixação biológica de nitrogênio; FBN; Biological nitrogen fixation; BNF.; Agrotóxico; Infecção; Nodulação; Micorriza Vesicular Arbuscular; Planta Forrageira; Rhizobium; Simbiose.; Infection; Nodulation; Pastures; Pesticides; Symbiosis; Vesicular arbuscular mycorrhizae.
Ano: 1992
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/623721

Ocorrência de bactérias do gênero Azospirillum spp. associadas a gramíneas forrageiras no Pantanal da Nhecolândia.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: BRASIL, M. da S.; FERNANDES, F. A.; BALDANI, V. L. D.
bitstream/CNPAB-2010/27176/1/cot046.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Bactéria diazotrófica; Fixação biológica de nitrogênio; FBN; Azospirillum spp; Diazotrophy bacteria; Biological nitrogen fixation; BNF.; Gramínea Forrageira.; Forage grasses.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/624139

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: González,Tehuni Orlando; Campanharo,João Carlos; Lemos,Eliana Gertrudes de Macedo.
This study aimed to genetically characterize four new Rhizobium strains, and to evaluate their nodulation and fixation capacity compared to commercial strains and to native rhizobia population of a Brazilian Rhodic Hapludox. Two experiments were carried out in randomized blocks design, under greenhouse conditions, in 2007. In the first experiment, the nodulation and nitrogen fixation capacity of new strains were evaluated, in comparison to the commercial strains CIAT-899 and PRF-81 and to native soil population. It was carried out in plastic tubes filled with vermiculite. DNA extractions and PCR sequencing of the intergenic space were made from the isolated pure colonies, in order to genetically characterize the strains and the native rhizobia population....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Biological nitrogen fixation; Intergenic space; Phylogeny; Sequencing; Soil rhizobia population.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000900012

Polymers as carriers for rhizobial inoculant formulations
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Fernandes Júnior,Paulo Ivan; Rohr,Tiago Gusmão; Oliveira,Paulo Jansen de; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa.
The aim of this work was to evaluate the efficiency of carboxymethyl cellulose (CMC) and starch blends as carrier materials of rhizobial inoculants regarding their capacity to maintain viable cells and promote cowpea (Vigna unguiculata) nodulation. The experimental design adopted was completely randomized, with three replicates. Forty different compositions of carboxymethyl cellulose (CMC) with starch, compatibilized or not with different proportions of MgO or ZnO, were evaluated regarding their ability of maintaining rhizobial viable cells during the storage period of one month at room temperature, in an initial screening. Thereafter, selected inoculant carrier blends were evaluated regarding their ability to maintain viable rhizobial cells for a period...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradyrhizobium japonicum; Vigna unguiculata; Biological nitrogen fixation; Carboxymethyl cellulose; Inoculant technology; Polymer blends.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000900017

Efficient inoculation of diazotrophic bacteria into sugarcane by the drip irrigation system
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Simões,Welson Lima; Oliveira,Anderson Ramos de; Souza,Moises Alves de; Reis,Verônica Massena; Ivan Fernandes-Júnior,Paulo.
Abstract: The objective of this work was to assess the efficiency of the inoculation of a diazotrophic bacterial consortium by the drip irrigation system into sugarcane (Saccharum spp.), in the Brazilian semiarid region. The evaluated treatments were: standard inoculation by submersion; one or two inoculations by drip irrigation; nitrogen (urea) applied by fertigation; and negative controls, without inoculation or nitrogen fertilization. Leaf gas exchanges and plant growth and yield were assessed. Inoculation by irrigation is efficient for all variables, and double inoculation by the drip irrigation system stands out, increasing stem yield in 16% when compared with the negative control, without differing from the treatment with nitrogen fertilizer.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Brazilian semiarid region; Inoculant; Plant-growth promoting bacteria.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000103304

Nitrogen symbiotically fixed by cowpea and gliricidia in traditional and agroforestry systems under semiarid conditions
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Martins,Júlio César Rodrigues; Freitas,Ana Dolores Santiago de; Menezes,Rômulo Simões Cezar; Sampaio,Everardo Valadares de Sá Barretto.
The objective of this work was to estimate the amounts of N fixed by cowpea in a traditional system and by cowpea and gliricidia in an agroforestry system in the Brazilian Northeast semiarid. The experiment was carried out in a randomized complete block design, in a split-plot arrangement, with four replicates, in the semiarid region of the state of Paraíba, Brazil. Plots consisted of agroforestry and traditional systems (no trees), and split-plots of the three crops planted between the tree rows in the agroforestry system. To estimate N fixation, plant samples were collected in the fourth growth cycle of the perennial species and in the fourth planting cycle of the annual species. In the agroforestry system with buffel grass and prickly-pear cactus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gliricidia sepium; Vigna unguiculata; Biological nitrogen fixation; Natural abundance; 15N.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000200178

Wheat yield in the Cerrado as affected by nitrogen fertilization and inoculation with Azospirillum brasilense
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Galindo,Fernando Shintate; Teixeira Filho,Marcelo Carvalho Minhoto; Buzetti,Salatiér; Santini,José Mateus Kondo; Alves,Cleiton José; Ludkiewicz,Mariana Gaioto Ziolkowski.
Abstract: The objective of this work was to evaluate the effect of inoculation with Azospirillum brasilense, associated with N rates and sources, in soil of the Cerrado (Brazilian savanna), on the grain yield of irrigated wheat (Triticum aestivum). The experiment was carried out under a no-tillage system in a Typic Haplustox. The experimental design was randomized complete blocks with four replicates, in a 2x5x2 factorial arrangement: two N sources (urea and urea with NBPT urease inhibitor); five N rates applied as topdressing (0, 50, 100, 150, and 200 kg ha-1); and with or without seed inoculation with A.brasilense. The increase in the N rates positively affected spike length, number of spikelets and of grains per spike, number of spikes per meter, N...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Biological nitrogen fixation; Nitrogen sources; No-tillage system; Urease inhibitor.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000900794

Comparison of 15N isotope methods to determine the recovery efficiency of nitrogen from green manure
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Araújo,Ednaldo da Silva; Silva,Bárbara Fernanda da; Chieza,Emerson Dalla; Urquiaga,Segundo; Guerra,José Guilherme Marinho; Costa,Janaína Ribeiro; Espíndola,Jose Antonio Azevedo.
Abstract: The objective of this work was to compare three methods of 15N isotope application to the soil in order to determine the recovery efficiency of nitrogen derived from green manure by corn (Zea mays). The used experimental design was a randomized complete block with six replicates. The treatments consisted of three 15N isotope methods: indirect method I, isotope dilution with 15N-labelled synthetic fertilizer applied through a small hole next to each corn plant; indirect method II, isotope dilution with 15N-labelled synthetic fertilizer spread in the furrow next to the corn planting row; and direct method, application of a legume straw as green manure, treated with 2.23% excess 15N. The green manure used was jack bean (Canavalia ensiformis)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Biological nitrogen fixation; Legume.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000103201

Elephant grass genotypes for bioenergy production by direct biomass combustion
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Morais,Rafael Fiusa de; Souza,Bruno Juscelino de; Leite,José Marcos; Soares,Luis Henrique de Barros; Alves,Bruno José Rodrigues; Boddey,Robert Michael; Urquiaga,Segundo.
The objective of this work was to evaluate elephant grass (Pennisetum purpureum Schum.) genotypes for bioenergy production by direct biomass combustion. Five elephant grass genotypes grown in two different soil types, both of low fertility, were evaluated. The experiment was carried out at Embrapa Agrobiologia field station in Seropédica, RJ, Brazil. The design was in randomized complete blocks, with split plots and four replicates. The genotypes studied were Cameroon, Bag 02, Gramafante, Roxo and CNPGL F06-3. Evaluations were made for biomass production, total biomass nitrogen, biomass nitrogen from biological fixation, carbon/nitrogen and stem/leaf ratios, and contents of fiber, lignin, cellulose and ash. The dry matter yields ranged from 45 to 67 Mg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pennisetum purpureum; Ash; Agroenergy; Biological nitrogen fixation; Genotype; Lignin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000200004

Green manure incorporation timing for organically grown broccoli
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Diniz,Ellen Rúbia; Santos,Ricardo Henrique Silva; Urquiaga,Segundo Sacramento; Peternelli,Luiz Alexandre; Barrella,Tatiana Pires; Freitas,Gilberto Bernardo de.
The objective of this work was to determine the effect of incorporation timing of the velvet bean (Stizolobium cinereum) (GM) on both organic broccoli yield and N status. Mineral N content in the soil, biologically fixed N recovery by broccoli, GM biomass decomposition and N release kinetics were also determined. Plots were fertilized with 12 Mg ha-1 of organic compost and received GM either at 0, 15, 30 or 45 days after transplant. Other treatments were compost (12 or 25 Mg ha-1), GM, mineral fertilizers and control (no fertilizer). The data were collected in four completely randomized blocks. GM decomposition increased mineral N content in soil as rapidly as mineral fertilizer or the supply of 25 Mg ha-1 of compost. The N half-life in GM (24 days) is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassica oleracea; Stizolobium cinereum; Biological nitrogen fixation; Decomposition rate; N recovery.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000200008

Diversity and nitrogen fixation efficiency of rhizobia isolated from nodules of Centrolobium paraense
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Baraúna,Alexandre Cardoso; Silva,Krisle da; Pereira,Gilmara Maria Duarte; Kaminski,Paulo Emílio; Perin,Liamara; Zilli,Jerri Edson.
The objective of this work was to isolate and characterize rhizobia from nodules of Centrolobium paraense and to evaluate their symbiotic efficiency. Soil samples collected from four sites of the Roraima Cerrado, Brazil, were used to cultivate C. paraense in order to obtain nodules. Isolates (178) were obtained from 334 nodules after cultivation on medium 79. Twenty-five isolates belonging to six morphological groups were authenticated using Vigna unguiculata and they were characterized by 16S rRNA. Isolates identified as Bradyrhizobium were further characterized using rpoB gene sequencing. A greenhouse experiment was carried out with C. paraense to test the 18 authenticated isolates. Approximately 90% of the isolates grew slowly in medium 79. The 16S rRNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradyrhizobium; Rhizobium; Bacteria diversity; Biological nitrogen fixation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2014000400296

Variability of nodulation traits in Andean and Mesoamerican common bean gene pools
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Knupp,Adriano Moreira; Ferreira,Enderson Petrônio de Brito; Araújo,Adelson Paulo.
Abstract: The objective of this work was to evaluate the genotypic diversity for nodulation in common bean (Phaseolus vulgaris) genotypes of Andean and Mesoamerican gene pools present in the core collections of Embrapa, in Brazil. Evaluations were carried out in two stages: the first one with 879 genotypes, taking the cultivar 'Ouro Negro' as reference; and the second one with 116 genotypes with greater nodulation, to identify those showing stability in nodulation in the two stages. Rhizobium strains were inoculated in pre-germinated common bean seed. Plants received weekly a nutrient solution without N, and nodulation was evaluated 35 days after planting. The percentage of genotypes that exceeded the reference cultivar was 22% for number of nodules, 46%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Biological nitrogen fixation; Diversity; Germplasm bank.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000400252

Symbiotic effectiveness of Bradyrhizobium ingae in promoting growth of Inga edulis Mart. seedlings
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Porto,Dilacy Sales; Farias,Eliane do Nascimento Cunha; Chaves,Josimar da Silva; Souza,Brenda Ferreira; Medeiros,Roberto Dantas de; Zilli,Jerri Édson; Silva,Krisle da.
ABSTRACT Inga edulis Mart. is a leguminous tree adapted to acidic and low-fertility soils that establishes symbioses with nitrogen (N)-fixing bacteria. The identification of effective bacteria in biological N fixation may bolster the use of I. edulis in degraded or modified areas and agroforestry systems. Therefore, the aims of this study were evaluation of the symbiotic effectiveness of eight strains of the Bradyrhizobium genus native to Roraima in Inga edulis plants, and in vitro evaluation of the ability of the eight strains of Bradyrhizobium to develop plant growth-promoting characteristics. Determination of symbiotic effectiveness was carried out via three experiments: the first in a greenhouse in pots with a sterile substrate; the second in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Phosphate solubilization; Indolic compounds.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100403

THE RESURRECTION PLANT TRIPOGON SPICATUS (POACEAE) HARBORS A DIVERSITY OF PLANT GROWTH PROMOTING BACTERIA IN NORTHEASTERN BRAZILIAN CAATINGA
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Fernandes-Júnior,Paulo Ivan; Aidar,Saulo de Tarso; Morgante,Carolina Vianna; Gava,Carlos Alberto Tuão; Zilli,Jerri Édson; Souza,Layane Silva Barbosa de; Marinho,Rita de Cássia Nunes; Nóbrega,Rafaela Simão Abrahão; Brasil,Marivaine da Silva; Seido,Sirando Lima; Martins,Lindete Míria Vieira.
Plant species that naturally occur in the Brazilian Caatinga(xeric shrubland) adapt in several ways to these harsh conditions, and that can be exploited to increase crop production. Among the strategic adaptations to confront low water availability, desiccation tolerance stands out. Up to now, the association of those species with beneficial soil microorganisms is not well understood. The aim of this study was to characterize Tripogon spicatusdiazotrophic bacterial isolates from the Caatingabiome and evaluate their ability to promote plant growth in rice. Sixteen bacterial isolates were studied in regard to their taxonomic position by partial sequencing of the 16S rRNA gene, putative diazotrophic capacity, in vitro indole-acetic acid (IAA) production and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Inoculant; Diazotrophic bacteria; Semi-arid; Desiccation tolerant plants.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000400993

Biodiversity of rhizobia associated with cowpea cultivars in soils of the lower half of the São Francisco River Valley
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Leite,Jakson; Seido,Sirando Lima; Passos,Samuel Ribeiro; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa; Martins,Lindete Míria Vieira.
The biodiversity of rhizobium in soils of the São Francisco Valley is unknown and can be studied using cowpea as trap plants. The objective of this study was to verify the diversity of diazotrophic bacteria that nodulate cowpea in soils of the lower half of the São Francisco River Valley by morphological and genotypic characterization. Seven soil samples (A1, A2, A3, A4, C1, C2 and MC) were collected to capture bacteria associated to five cowpea cultivars (IPA 206, BRS Pujante, BRS Marataoã, Canapu Roxo, and Sempre Verde), in a 5x7 factorial design with three replications. Thirty days after plant emergence, the nodules were collected and the bacteria isolated and analyzed in relation to their growth characteristics in YMA medium. The 581 isolates were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ARDRA; Biological nitrogen fixation; Vigna unguiculata.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500015

Soybean response to starter nitrogen and Bradyrhizobium inoculation on a Cerrado oxisol under no-tillage and conventional tillage systems
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Mendes,I. C.; Hungria,M.; Vargas,M. A. T.
In Brazil, Bradyrhizobium inoculation has successfully replaced the use of N fertilizer on soybean [Glycine max (L) Merr.] crops. However, with the expansion of no-tillage cropping systems in the Cerrados region, the idea that it is necessary to use small N rates at the sowing to overcome problems related with N immobilization has become widespread, mainly when soybean is cultivated after a non-legume crop. In this study we examined soybean response to small rates of N fertilizer under no-tillage (NT) and conventional tillage (CT) systems. Four experiments (a completely randomized block with five replicates) were carried out in a red yellow oxisol, during the periods of 1998/1999 and 1999/ 2000, under NT and CT. The treatments consisted of four urea rates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Bradyrhizobium japonicum; Inoculation; Zero-tillage; Biological nitrogen fixation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832003000100009

Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Menezes,Kelly Alexsandra Souza; Escobar,Indra Elena Costa; Fraiz,Ana Carla Resende; Martins,Lindete Míria Vieira; Fernandes Júnior,Paulo Ivan.
ABSTRACT Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from “mulungu” (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; “mulungu”; Tree legumes; “Caatinga”; Rhizobia.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100404

Inoculation of sugarcane with diazotrophic bacteria
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Schultz,Nivaldo; Silva,Jeferson Alves da; Sousa,Jailson Silva; Monteiro,Rafael Cassador; Oliveira,Renan Pedula; Chaves,Valfredo Almeida; Pereira,Willian; Silva,Marinete Flores da; Baldani,José Ivo; Boddey,Robert Michael; Reis,Veronica Massena; Urquiaga,Segundo.
The sugarcane industry, a strategic crop in Brazil, requires technological improvements in production efficiency to increase the crop energy balance. Among the various currently studied alternatives, inoculation with diazotrophic bacteria proved to be a technology with great potential. In this context, the efficiency of a mixture of bacterial inoculant was evaluated with regard to the agronomic performance and N nutrition of sugarcane. The experiment was carried out on an experimental field of Embrapa Agrobiologia, in Seropédica, Rio de Janeiro, using a randomized block, 2 × 3 factorial design (two varieties and three treatments) with four replications, totaling 24 plots. The varieties RB867515 and RB72454 were tested in treatments consisting of:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharum sp; Inoculant; Biological nitrogen fixation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832014000200005

Bradyrhizobium spp. Strains in Symbiosis with Pigeon Pea cv. Fava-Larga under Greenhouse and Field Conditions
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Rufini,Márcia; Oliveira,Dâmiany Pádua; Trochmann,André; Soares,Bruno Lima; Andrade,Messias José Bastos de; Moreira,Fatima Maria de Souza.
ABSTRACT: Optimization of symbiosis between nitrogen-fixing bacteria and legumes has been extensively studied, seeking agricultural sustainability. To evaluate the symbiotic efficiency of nitrogen-fixing bacterial strains belonging to the Bradyrhizobium genus with pigeon pea (Cajanus cajan (L.) Millsp.) cv. Fava-Larga, experiments were conducted in Leonard jars (axenic conditions), pots with soil, and in the field. Ten strains were tested in Leonard jars, and three strains, in addition to BR 29, were selected according to their ability to promote the growth of pigeon pea, for further tests in pots with different soil types (Inceptsol and Oxisol) and in the field (Oxisol). Treatments were compared with strains BR 2003 and BR 2801 (approved as inoculants for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cajanus cajan; Inoculant; Biological nitrogen fixation; Selection.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832016000100432

New Native Rhizobia Strains for Inoculation of Common Bean in the Brazilian Savanna
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Mercante,Fábio Martins; Otsubo,Auro Akio; Brito,Osmar Rodrigues.
ABSTRACT Maximization of biological nitrogen fixation in the common bean (Phaseolus vulgaris L.) crop depends on the genetic characteristics related to the plant, the symbiotic efficiency of rhizobia, and environmental factors. The objective of this study was to evaluate the performance of rhizobia selected beforehand from Cerrado (Brazilian tropical savanna) soils in Mato Grosso do Sul. The experiments were conducted in 2007 in the municipalities of Aquidauana, Anaurilândia, Campo Grande, and Dourados, all located in the state of Mato Grosso do Sul. All procedures established followed the current recommendations of the Brazilian Ministry of Agriculture (Ministério de Agricultura, Pecuária e Abastecimento – MAPA), in accordance with the “official protocol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Rhizobium tropici; Microbial inoculants; Biological nitrogen fixation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100405

Mitigation of Mombasa Grass ( Megathyrsus maximus ) Dependence on Nitrogen Fertilization as a Function of Inoculation with Azospirillum brasilense
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Leite,Rubson da Costa; Santos,Antonio Clementino dos; Santos,José Geraldo Donizetti dos; Leite,Robson da Costa; Oliveira,Leonardo Bernardes Taverny de; Hungria,Mariangela.
ABSTRACT Using biological inputs to improve the efficiency of nitrogen fertilizers represents an alternative for the cultivation of grasses in tropical regions. Azospirillum brasilense is a species of plant growth promoting bacteria widely studied and used in inoculants. Thus, this study aimed to evaluate the performance of Mombasa grass ( Megathyrsus maximus ) in association with A. brasilense and nitrogen (N) fertilization. The study was conducted under field conditions in Araguaína-Tocantins State, between December 2017 and May 2018. The treatments were arranged in randomized blocks, in a 5 × 2 factorial arrangement, with five doses of N fertilization (0, 25, 50, 75, and 100 kg ha-1) combined with two inoculation treatments (inoculated and non...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth-promoting microbes; Inoculant; Biological nitrogen fixation; Tropical pasture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832019000100523

Technical and economic viability of co-inoculation with Azospirillum brasilense in soybean cultivars in the Cerrado
Provedor de dados: Revista Brasileira de Engenharia Agrícola e Ambiental
Autores: Galindo,Fernando S.; Teixeira Filho,Marcelo C. M.; Buzetti,Salatier; Ludkiewicz,Mariana G. Z.; Rosa,Poliana A. L.; Tritapepe,Caio A.
ABSTRACT Biological nitrogen fixation (BNF) efficiency can be increased by co-inoculation with bradyrhizobia and Azospirillum brasilense, allowing even greater uptake of water and nutrients, leading to higher yields. Thus, this study aimed to evaluate the technical and economic viability of soybean in the Cerrado, according to the cultivars and co-inoculation with Azospirillum brasilense. The experiment was conducted in Selvíria, MS, in no-tillage system, in Oxisol, arranged in a randomized block design in a 2 x 2 factorial scheme with two cultivars (‘Potência’ and ‘Valiosa’), with and without co-inoculation with Azospirillum brasilense in the seed. Co-inoculation with A. brasilense increases grain yield in the cultivars ‘Potência’ and ‘Valiosa’, being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Total operating cost; Glycine max; Technical performance; Economic performance.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662018000100051

Inoculation efficiency of Azospirillum brasilense on economising nitrogen fertiliser in landrace popcorn
Provedor de dados: Revista Ciência Agronômica (RCA)
Autores: Martins,Tainara Gomes; Freitas Júnior,Silvério Paiva; Luz,Lucas Nunes; Marco,Cláudia Araújo; Vásquez,Edilza Maria Felipe.
ABSTRACT Nitrogen is one of the nutrients most demandedby popcorn, and one that most increases production costs. Considering these factors, the aim of this study was to evaluate the effectiveness of inoculatingpopcorn seeds with Azospirillum brasilense together with four levels of nitrogen fertiliser, and its contribution to characteristics related to production. The experiment was carried out in the experimental area of the Centre for Agrarian Sciences and Biodiversity of the Federal University of Cariri, in Crato, in the State of Ceará. Eight treatments were evaluated with four replications, using an experimental design of randomised blocks in a 2x4 factorial scheme corresponding to the presence and absence of inoculation, and to four doses of nitrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diazotrophic bacteria; Biological nitrogen fixation; Nitrogen fertiliser.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000200283

Seed quality, chlorophyll content index and leaf nitrogen levels in maize inoculated with Azospirillum brasilense
Provedor de dados: Revista Ciência Agronômica (RCA)
Autores: Pereira,Lívia de Matos; Pereira,Elise de Matos; Revolti,Lucas Tadeu Mazza; Zingaretti,Sonia Marli; Môro,Gustavo Vitti.
ABSTRACTThe aim of this study was to evaluate differences between maize genotypes in relation to the germination response of the seeds and the growth of seedlings inoculated with Azospirillum brasilense, as well as the effect of inoculation on nitrogen levels and the chlorophyll content index of the leaves. The physiological seeds quality from the single-cross hybrids AG7098 and 2B707, and from the experimental synthetic varieties V2 and V4, inoculated with A. brasilense, was tested for germination, percentage and rate of emergence, and dry matter weight of the shoots and roots. Nitrogen levels and chlorophyll content index were evaluated in leaves of the same four genotypes grown in a greenhouse under different nitrogen supply systems and methods of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Diazotrophic bacteria; Biological nitrogen fixation; Plant-bacteria interaction.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902015000300630

Interactions between diazotrophic bacteria and mycorrhizal fungus in maize genotypes
Provedor de dados: Scientia Agricola
Autores: Miyauchi,Marina Yumi Horta; Lima,Dáfila Santos; Nogueira,Marco Antonio; Lovato,Gisele Milani; Murate,Letícia Sayuri; Cruz,Márcio Ferreira; Ferreira,Josué Maldonado; Zangaro,Waldemar; Andrade,Galdino.
Some diazotrophic bacteria can fix nitrogen biologically in gramineous host plants. Generally, gramineous plants are also associated with mycorrhizal fungi, that can improve mainly plant P uptake. Among the factors affecting plant-microbe interactions, the plant genotype plays an important role. This study evaluates the effect of diazotrophic bacteria and an arbuscular mycorrhizal fungus (AMF), on five genotypes of maize (Zea mays L.), in relation to plant biomass, shoot N and P concentrations, and fine root morphological traits. The experimental design was entirely randomized in a factorial 5 × 4 × 2 arrangement, i.e., five maize genotypes (hybrids C333B, AS3466, and PREMIUM, and the inbreed lines lg40897-1 and lg40505-1), three diazotrophic bacteria...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azospirillum; Biological nitrogen fixation; Mycorrhiza; Root morphology.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500012

Bacterial strains from floodplain soils perform different plant-growth promoting processes and enhance cowpea growth
Provedor de dados: Scientia Agricola
Autores: Costa,Elaine Martins da; Carvalho,Fernanda de; Nóbrega,Rafaela Simão Abrahão; Silva,Jacqueline Savana; Moreira,Fatima Maria de Souza.
ABSTRACT Certain nodulating nitrogen-fixing bacteria in legumes and other nodule endophytes perform different plant-growth promoting processes. The objective of this study was to evaluate 26 bacterial strains isolated from cowpea nodules grown in floodplain soils in the Brazilian savannas, regarding performance of plant-growth promoting processes and ability to enhance cowpea growth. We also identified these strains by 16S rRNA sequencing. The following processes were evaluated: free-living biological nitrogen fixation (BNF), solubilization of calcium, aluminum and iron phosphates and production of indole-3-acetic acid (IAA). The abilities to nodulate and promote cowpea growth were evaluated in Leonard jars. Partial sequencing of the 16S rRNA gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paenibacillus; Biotechnological potential; Biological nitrogen fixation; Phosphate solubilization; Indole-3-acetic acid.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000400301

Plant densities and modulation of symbiotic nitrogen fixation in soybean
Provedor de dados: Scientia Agricola
Autores: Luca,Marcos Javier de; Hungría,Mariangela.
Soybean nitrogen (N) demands can be supplied to a large extent via biological nitrogen fixation, but the mechanisms of source/sink regulating photosynthesis/nitrogen fixation in high yielding cultivars and current crop management arrangements need to be investigated. We investigated the modulation of symbiotic nitrogen fixation in soybean [Glycine max (L.) Merrill] at different plant densities. A field trial was performed in southern Brazil with six treatments, including non-inoculated controls without and with N-fertilizer, both at a density of 320,000 plants ha−1, and plants inoculated with Bradyrhizobium elkanii at four densities, ranging from 40,000 to 320,000 plants ha−1. Differences in nodulation, biomass production, N accumulation and partition were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Biological nitrogen fixation; Photosynthesis; Source/sink manipulation; Plant density.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000300002

Alfalfa yield and quality as function of nitrogen fertilization and symbiosis with Sinorhizobium meliloti
Provedor de dados: Scientia Agricola
Autores: Oliveira,Wladecir Salles de; Oliveira,Patrícia Perondi Anchão; Corsi,Moacyr; Duarte,Fábio Rodrigo Sanches; Tsai,Siu Mui.
The efficiency of the symbiotic process between alfalfa (Medicago sativa L.) and Sinorhizobium meliloti can be drastically affected by soil nitrogen. This research evaluates how mineral fertilization affects the contribution of symbiosis to the yield and quality of alfalfa in two experiments under controlled conditions, and a third one in the field. Under controlled conditions, positive effect of nitrogen fertilizer was observed, whereas, in the field, negative effect of the mineral nitrogen (450 kg ha-1 year-1) on the symbiotic process was observed, with reduction of nodulation and nitrogenase activity. However, there was no effect on yield, total nitrogen concentration, crude protein, non-protein nitrogen and digestibility in relation to the treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicago sativa L.; Biological nitrogen fixation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400013

Sunnhemp and millet as green manure for tropical maize production
Provedor de dados: Scientia Agricola
Autores: Perin,Adriano; Santos,Ricardo Henrique Silva; Urquiaga,Segundo Sacramento; Cecon,Paulo Roberto; Guerra,José Guilherme Marinho; Freitas,Gilberto Bernardo de.
Commonly N release from legume residues is rapid in the tropics. Legume mixtures with grasses can result in slower nutrient release from residues, better matching the N requirements of the following crop. The objectives of this study were to evaluate the effects of the green manures sunnhemp (Crotalaria juncea) and millet (Pennisetum glaucum), alone or mixed, on the performance of a subsequent maize crop with and without the application of N-fertilizer. The experiment consisted of four randomized blocks, with split plots. The plot treatments consisted of previous cultivation of sunnhemp, millet, mixed sunnhemp+millet and weeds. The subplot treatments were 90 kg N ha-1 and absence of N-fertilizer. Substantial amounts of biological nitrogen fixation (BNF)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N recovery; Biological nitrogen fixation; Decomposition rate; Intercropping.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000500006

Growth rate and nutritional status of an organic coffee cropping system
Provedor de dados: Scientia Agricola
Autores: Ricci,Marta dos Santos Freire; Alves,Bruno José Rodrigues; Miranda,Simone Cordeiro de; Oliveira,Fabio Freire de.
In view of the low N concentration in organic fertilizers, it is necessary to use high rates of such fertilizers to attend coffee crop requirements. Hence, N is the most limiting nutrient for organic coffee production. The objective of this work was to evaluate the influence of sunn hemp (Crotalaria juncea) organic fertilization on the growth and nutritional status of coffee cultivars, as well as to quantify plant biomass and N input derived from biological nitrogen fixation, and their effect on soil chemical characteristics. The experiment consisted of six coffee (Coffea arabica) cultivars intercropped with and without sunn hemp sown in November 2001 and pruned at mid-height 76 days later. At 175 days, the standing biomass of the legume was cut, measuring...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Crotalaria juncea; Biological nitrogen fixation; Nutrient input; Green manure.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200008

Crop rotation biomass and arbuscular mycorrhizal fungi effects on sugarcane yield
Provedor de dados: Scientia Agricola
Autores: Ambrosano,Edmilson José; Azcón,Rozario; Cantarella,Heitor; Ambrosano,Gláucia Maria Bovi; Schammass,Eliana Aparecida; Muraoka,Takashi; Trivelin,Paulo César Ocheuze; Rossi,Fabrício; Guirado,Nivaldo; Ungaro,Maria Regina Gonçalves; Teramoto,Juliana Rolim Salomé.
A cana-de-açúcar (Saccharum spp.) vem sendo cultivada no Brasil para produção de açúcar e agroenergia. Em seu sistema de produção, após um ciclo de 4 a 8 anos, é possível a rotação com plantas de cobertura, antes do seu replantio, para melhoria do solo e geração de renda. Estudou-se a caracterização e produtividade de biomassa de leguminosas (como adubos-verdes) e girassol (Helianthus annuus L.), a ocorrência natural de micorrizas, o teor de açúcar e a produtividade em colmos da cana-de-açúcar IAC 87-3396 e a viabilidade econômica desse sistema com cultivo após as opções de rotação, com quantificação da produtividade durante três cortes consecutivos. O amendoim (Arachis hypogaea L.) cv. IAC-Caiapó, girassol cv. IAC-Uruguai e mucuna-preta (Mucuna aterrimum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Green manure; Legumes; Biological nitrogen fixation; Sugarcane stalk yield.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000600011

Occurrence of diazotrophic bacteria in Araucaria angustifolia
Provedor de dados: Scientia Agricola
Autores: Neroni,Rafaela de Fátima; Cardoso,Elke Jurandy Bran Nogueira.
Araucaria angustifolia is an environmentally threatened tree and the whole biota of the Araucaria Forest should be investigated with the aim of its preservation. Diazotrophic bacteria are extremely important for the maintenance of ecosystems, but they have never been studied in Araucaria Forests. In this study, diazotrophic bacteria were isolated from Araucaria roots and soil, when grown in semi-specific, semi-solid media. The diazotrophic character of some recovered isolates could be confirmed using the acetylene reduction assay. According to their 16S rRNA sequences, most of these isolates belong to the genus Burkholderia.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Biological nitrogen fixation; Endophytic bacteria; Rhizosphere; Forest.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000300015

Diazotrophic bacteria and substrates in the growth and nitrogen accumulation of sugarcane seedlings
Provedor de dados: Scientia Agricola
Autores: Matoso,Ester Schiavon; Reis,Veronica Massena; Giacomini,Sandro José; Silva,Mariana Teixeira da; Avancini,Anita Ribas; Silva,Sérgio Delmar dos Anjos e.
ABSTRACT Little is known about the interaction between the inoculation of diazotrophic bacteria, the variety, and the substrate used in inoculated sugarcane seedling production. Bearing this in mind, the aim of this study was to evaluate the effects of diazotrophic bacteria inoculation, four sugarcane varieties and four different substrates on the growth and nitrogen accumulation of sugarcane seedlings. Parameters related to sprouting, height, and root growth, as well as nitrogen accumulation, were evaluated. The results indicate that inoculating sugarcane seedlings belonging to the following varieties: RB867515, RB92579, RB966928 and RB975932 with bacteria may increase sprouting speed index, height, root length, fresh and dry matter weight, as well as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharum sp.; Carbonized rice husk; Organic compost; Biological nitrogen fixation.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000101403

Inoculation of plant growth promoting rhizobia in Sudan grass (Sorghum χ sudanense (Piper) Stapf cv. Sudanense) and millet (Pennisetum glaucum (L.) R.Br. cv. BRS1501)
Provedor de dados: Acta Agronómica
Autores: Goulart-Machado,Rafael; Saccol-de Sá,Enilson Luiz; Hahn,Leandro; Pilatti-Sant'Ana,Wilian Luan.
Abstract Rhizobia are able to increase yield of non-leguminous species through production of phyto-stimulating substances. This study aimed to evaluate the inoculation effect of rhizobia UFRGS Lc348 and VP16 on millet and Sudan grass yield and germination, and verify the enrichment effect of culture medium with tryptophan, which leads on the rhizobium/plant interaction. Experiments in vitro and greenhouse conditions were conducted. In millet, the inoculation with VP16 grown in culture medium with or without tryptophan induces greater length of hypocotyl and epicotyl under in vitro conditions. UFRGS Lc348 treatment induces longer hypocotyls of millet under in vitro conditions. No effects were observed with the millet inoculation in greenhouse. In Sudan...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Forage grasses; Indole acetic acid (IAA); Rhizobacteria; Root nodules and symbiont; Soil microorganism.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000100133

Biological fixation of nitrogen by native isolates of Rhizobium sp. symbionts of Leucaena leucocephala (Lam.) De Wit
Provedor de dados: Acta Agronómica
Autores: Cubillos-Hinojosa,Juan Guillermo; Milian-Mindiola,Pablo Ernesto; Hernández-Mulford,Jorge Luis; Peralta-Castilla,Arnaldo.
Abstract Forage legumes such as Leucaena leucocephada, besides being a rich source of protein for animal feed, its inclusion in silvopastoral systems provides fundamental components to improve soil properties. These plants have the ability to establish symbiotic association with the genus Rhizobium sp. and fix biologically atmospheric nitrogen favoring growth and development, being reflected in production increases. The objective of this research was to evaluate the capacity of native isolates of Rhizobium sp. and a commercial strain in biological nitrogen fixation (BNF) in L. leucocephala plants. Two separate bioassays to evaluate the efficiency of the FBN of the native isolates and the commercial strain under greenhouse conditions, followed by a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leucaena leucocephala; Biological nitrogen fixation; Forage legumes; Rhizobium sp.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122019000200075

Characterization of bacterial endophytes from the roots of native and cultivated Brazil nut trees (Bertholletia excelsa)
Provedor de dados: Acta Amazonica
Autores: CHALITA,Patrícia Bombonati; FARIAS,Eliane do Nascimento Cunha; COSTA,Ismaele Breckenfeld da; SOUSA,Brenda Ferreira; SANTOS,Marco Antônio Oliveira dos; ALBUQUERQUE,Teresinha Costa Silveira de; VITAL,Marcos José Salgado; SILVA,Krisle da.
ABSTRACT Brazil nut is a very important nontimber forest product in the Amazon region. Propagation of this tree still represents a challenge due to slow and uneven seed germination. In this context, plant growth-promoting bacteria can facilitate the process of propagation. The aims of this study were to isolate and characterize endophytic bacteria from the roots of Brazil nut trees in native terra firme forest and cultivation areas in northern Brazil, and to identify mechanisms by which bacteria act in plant growth promotion. Overall, 90 bacterial isolates were obtained from the roots of Brazil nut trees in monoculture, agroforestry and native forest areas by using different semisolid media. The isolates were characterized by sequencing the 16S rRNA gene....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 16S rRNA; Phosphate solubilization; Indole compounds; Biological nitrogen fixation; Plant growth-promotion bacteria; Amazon.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400257

Agronomic efficiency of Rhizobium strains from the Amazon region in common bean
Provedor de dados: Acta Amazonica
Autores: NOGUEIRA,Cláudia de Oliveira Gonçalves; OLIVEIRA,Dâmiany Pádua; FERREIRA,Paulo Ademar Avelar; PEREIRA,João Paulo de Andrade Rezende; VALE,Helson Mário Martins do; ANDRADE,Messias José Bastos de; MOREIRA,Fatima Maria de Souza.
ABSTRACT To maximize the contribution of biological nitrogen fixation in common bean, it is necessary to use bacterial strains that are more adapted, competitive, and efficient in the symbiotic process. In this regard, the aim of this study was to evaluate the agronomic efficiency (AE) of three bacterial strains isolated from acid soils with high Al content from the Amazon region in an Argissolo Vermelho Distrófico típico soil (Typic Rhodustults - USDA Classification) from the municipality of Formiga, MG, Brazil. We compared their AE to that of the reference strain CIAT 899T and of two controls without inoculation (one without and another with 80 kg ha-1 of N-urea). The results indicated that inoculation with the strains UFLA 02-100 and UFLA 02-127...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Phaseolus vulgaris L.; Rhizobia; Biological nitrogen fixation; Seed inoculation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000300273

Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region
Provedor de dados: Acta Amazonica
Autores: SILVA,Krisle da; PERIN,Liamara; GOMES,Maria de Lourdes; BARAÚNA,Alexandre Cardoso; PEREIRA,Gilmara Maria Duarte; MOSQUEIRA,Cátia Aparecida; COSTA,Ismaele Breckenfeld da; O'HARA,Graham; ZILLI,Jerri Édson.
ABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which seven...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Zea mays L.; 16S rRNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000200111

Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region
Provedor de dados: Acta Amazonica
Autores: MOREIRA,Fatima Maria de Souza; COELHO,Katia Pereira; RIBEIRO,Paula Rose de Almeida; GUIMARÃES,Amanda Azarias.
ABSTRACT A great number of species and individuals of scandent legumes establishing symbiosis with nitrogen fixing bacteria occurs in the Amazon Forest. These symbiosis probably play an important role in contributing to nitrogen incorporation in this ecossystem. The objectives of this study were to evaluate the growth of eight species of scandent legumes in five nursery substrates; to compare nodulation with rhizobia strains introduced or native to these substrates; and to characterize phenotypically and genetically these rhizobia. The experiment was carried out in a completely randomized design with five replications. Five to seven months after seedling emergency, according to the legume species, growth and nodulation parameters were determined. Rhizobia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forest species; Lianas; Biological nitrogen fixation; Bradyrhizobium.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000400367

Nitrogen and molybdenum fertilization and inoculation of common bean with Rhizobium spp. in two oxisols
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Figueiredo, Marislaine Alves de; Oliveira, Dâmiany Pádua; Soares, Bruno Lima; Morais, Augusto Ramalho de; Moreira, Fatima Maria de Souza; Andrade, Messias José Bastos de.
The aim of this study was to evaluate the effects of foliar application of molybdenum and small dose of nitrogen at planting on common bean production when using seed inoculation with rhizobia. Two field experiments were conducted, applying a randomized block design and a factorial arrangement (3x2x2) + 1, with three replicates. Treatments consisted of inoculation with Rhizobium spp. (strain CIAT 899T, strain UFLA 02–100, and uninoculated), foliar application of molybdenum (0 and 80 g ha-1), and fertilization with N-urea at planting (0 and 20 kg ha-1), with an additional N treatment of 40 applied at planting and 40 kg ha-1 applied as topdressing. The foliar application of molybdenum did not favor the nodulation, but its effect on yield was dependent on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AGRONOMIA (50100009) Phaseolus vulgaris L.; Biological nitrogen fixation; Nutrients MICROBIOLOGIA E BIOQUÍMICA DO SOLO.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/26661

Rhizobial diversity from stem and root nodules of Discolobium and Aeschynomene
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Martins, Claudia Miranda; Borges, Wardsson Lustrino; Costa Júnior, José de Souza; Rumjanek, Norma Gouvêa.
Among the genera that exhibit stem nodulation, Aeschynomene and Discolobium are found in the Pantanal Mato-grossense (wetlands in the state of Mato Grosso, Brazil). Isolates obtained from the stem and root nodules of D. pulchellum, D. psoraleaefolium, D. leptophyllum and A. fluminensis were collected from various locations in the Pantanal de Poconé sub-region and phenotypically characterized and genotyped by restriction analysis of the 16S rDNA gene. Of the 282 isolates obtained from the stem and root nodules, 84.3% alkalized YMA media and 74.1% showed slow growth. No differences in either the phenotype or the genotype among the rhizobial populations isolated from the root or stem nodules of the species Discolobium and A. fluminensis were observed. Among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciências Agrárias; Ciência do Solo Flooding-tolerant legume; Symbioses; Pantanal; 16S rDNA; Biological nitrogen fixation; PCR-RFLP Microbiologia do Solo.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19565

Components of corn crop yield under inoculation with Azospirillum brasilense using integrated crop-livestock system
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Brum, Marcos da Silva; Cunha, Vinícius Dos Santos; Stecca, Jéssica Deolinda Leivas; Grando, Luiz Fernando Teleken; Martin, Thomas Newton.
The objective of this study was to evaluate the agronomic characteristics of corn seed inoculated with Azospirillum brasilense, grown on black oat and ryegrass straw, and managed under different grazing strategies and doses of nitrogen. The experiment was conducted in Santa Maria, Rio Grande do Sul State, Brazil, during two agricultural seasons (2012/2013 and 2013/2014) in a randomized, complete block design with three replications. In the winter period, black oat and ryegrass straw were managed at different grazing heights by sheep (0.30, 0.20, 0.10 m, conventional grazing, and no grazing) with three doses of nitrogen (0, 50, and 100 kg ha-1, with or without inoculation by A. brasilense). We used the hybrid Pioneer (P1630H®) in 2012 and the hybrid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.01.00.00-9 Zea mays; Grazing heights; Biological nitrogen fixation; Nitrogen; Integrated systems. Fitotecnia.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30664

Understory structure in two successional stages of a Semi-deciduous Seasonal Forest remnant of Southern Brazil
Provedor de dados: Biota Neotropica
Autores: Coelho,Geraldo Ceni; Rigo,Marivana da Silva; Libardoni,Juliana Boniatti; Oliveira,Rosemeri de; Benvenuti-Ferreira,Glaci.
The knowledge of structure and composition of forest strata contributes to understanding the conservation status and dynamics of plant communities. The study of forest understory is important to the knowledge of the succession process. The Seasonal Subtropical Forest remnant named Mato do Silva (27º 55'26" S and 53º 53'15" W) was studied aiming to describe the structure of understory in the late successional forest (LF) and in the young secondary forest (SF), an 11 years old post-agricultural site with canopy dominance of Ateleia glazioveana Baill. The phytosociological survey was carried out through the point quarter method and included individuals of tree and shrub species from 0.5 to 3.0 m of height. The sampling consisted of 384 individuals in LF (67...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity conservation; Biological nitrogen fixation; Secondary succession; Atlantic forest of uruguai river basin; Subtropical forest.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032011000300004

Rhizobium strains selected from the Amazon region increase the yield of snap bean genotypes in protected cultivation
Provedor de dados: Bragantia
Autores: Oliveira,Dâmiany Pádua; Ferreira,Sindynara; Soares,Bruno Lima; Ferreira,Paulo Ademar Avelar; Morais,Augusto Ramalho de; Moreira,Fatima Maria de Souza; Andrade,Messias José Bastos de.
ABSTRACT Although the use of inoculants containing rhizobia is encouraged, there are no official recommendations for inoculation of snap bean. In this respect, the aim of this study was to evaluate the agronomic performance of Rhizobium strains in symbiosis with snap bean cultivars with different growth habits and crop cycles. The experiment was carried out in pots with soil in a greenhouse in the spring-summer season in Lavras, state of Minas Gerais, Brazil. A completely randomized experimental design was used, with 4 replications, in a 4 × 7 factorial arrangement involving four snap bean cultivars and seven treatments – five strains of Rhizobium (UFLA 02-100, UFLA 02-127, UFLA 04-173, CIAT 899, and PRF 81), plus two controls without inoculation (with and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Green bean cultivars; Phaseolus vulgaris; Rhizobium spp.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000200292

New rhizobia strains isolated from the Amazon region fix atmospheric nitrogen in symbiosis with cowpea and increase its yield
Provedor de dados: Bragantia
Autores: Ferreira,Paulo Ademar Avelar; Pereira,João Paulo Andrade Rezende; Oliveira,Dâmiany Pádua; Vale,Helson Mário Martins do; Jesus,Ederson da Conceição; Soares,André Luís de Lima; Nogueira,Cláudia de Oliveira Gonçalves; Andrade,Messias José Bastos de; Moreira,Fatima Maria de Souza.
ABSTRACT Studies in the Amazon indicate a wide diversity of rhizobia with the ability for biological nitrogen fixation (BNF), which could expand the number of strains approved for cowpea. Thus, the aim of this field study was to evaluate the agronomic performance in cowpea of the several strains isolated from the soils of the Brazilian states Acre and Rondônia, and to compare them withstrains approved by the Ministry of Agriculture (MAPA) and withnon-inoculated controls (without and with mineral nitrogen fertilizer). The inoculants performed well. Though less effective than the other strains, the UFLA 03-36 strain also was prominent with respect to grain yield. Because of the positive response of the UFLA 03-129 strain, which led to yield increases greater...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vigna unguiculata; Biological nitrogen fixation; Amazon strains; Seed inoculation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000100038

Symbiotic and agronomic efficiency of new cowpea rhizobia from Brazilian Semi-Arid
Provedor de dados: Bragantia
Autores: Marinho,Rita de Cássia Nunes; Ferreira,Linnajara de Vasconcelos Martins; Silva,Aleksandro Ferreira da; Martins,Lindete Míria Vieira; Nóbrega,Rafaela Simão Abrahão; Fernandes-Júnior,Paulo Ivan.
ABSTRACT Cowpea is a very important crop to Brazilian Semi-Arid mainly small family-based farmers. Rhizobia inoculation is a practice, easy to use, and cheap technology that increases cowpea productivity. The aim of this study was to evaluate the efficiency of two new rhizobia isolates in greenhouse and field as well as classify them taxonomically. To bacterial identification the 16S rRNA gene of ESA 17 and ESA 18 isolates were sequenced. The greenhouse test was conducted with pots containing 3 L of soil and the bacterial isolates evaluated were ESA 17, ESA 18, BR 3267 or BR 3262 strains. A field experiment was implemented on a Vertisol in Juazeiro, Bahia State, to evaluate the cowpea growth and productivity. In this experiment, the peat-based inoculants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradyrhizobium; Inoculant; Biological nitrogen fixation; Strain selection; São Francisco River Valley.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200273

Actual and potential heterotrophicneutrogen fixation and detrification rates of oryza glumaepatula Steud in an amazonian lake
Provedor de dados: Brazilian Journal of Biology
Autores: ENRICH-PRAST,A.; ESTEVES,F. A.; BIESBOER,D. D.
Estimates of the rates of heterotrophic nitrogen fixation (HNF) and denitrification by acetylene reduction and acetylene inhibition, respectively, were determined for the roots and culms of wild rice, Oryza glumaepatula, in Lake Batata, a lake severely impacted by the dumping of bauxite tailings into the lake basin. Two stations were established in different parts of the lake: one in a natural area and other in an impacted area of the lake. Plant assays were performed during the principle hydroperiods of dry (December) and flood (July) to determine both: a) Actual rates of HNF and denitrification, and b) potential rates of HNF after amending with glucose potential rates of denitrification after amending with glucose, glucose + nitrate, or nitrate alone....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Amazonian lake; Aquatic macrophyte.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71081999000300013

Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nishikawa,C.Y.; Araújo,L.M.; Kadowaki,M.A.S.; Monteiro,R.A.; Steffens,M.B.R.; Pedrosa,F.O.; Souza,E.M.; Chubatsu,L.S.
Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ54 co-factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Biological nitrogen fixation; Azospirillum brasilense; NifA protein.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200004

Effect of point mutations on Herbaspirillum seropedicae NifA activity
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Aquino,B.; Stefanello,A.A.; Oliveira,M.A.S.; Pedrosa,F.O.; Souza,E.M.; Monteiro,R.A.; Chubatsu,L.S.
NifA is the transcriptional activator of the nif genes in Proteobacteria. It is usually regulated by nitrogen and oxygen, allowing biological nitrogen fixation to occur under appropriate conditions. NifA proteins have a typical three-domain structure, including a regulatory N-terminal GAF domain, which is involved in control by fixed nitrogen and not strictly required for activity, a catalytic AAA+ central domain, which catalyzes open complex formation, and a C-terminal domain involved in DNA-binding. In Herbaspirillum seropedicae, a β-proteobacterium capable of colonizing Graminae of agricultural importance, NifA regulation by ammonium involves its N-terminal GAF domain and the signal transduction protein GlnK. When the GAF domain is removed, the protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Herbaspirillum seropedicae; NifA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800683

Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.]
Provedor de dados: Brazilian Journal of Microbiology
Autores: Fernandes Júnior,Paulo Ivan; Lima,Andréa Aparecida de; Passos,Samuel Ribeiro; Gava,Carlos Alberto Tuão; Oliveira,Paulo Jansen de; Rumjanek,Norma Gouvêa; Xavier,Gustavo Ribeiro.
This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strain selection; Biological nitrogen fixation; Biotechnology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400045

Draft genome sequence of Bradyrhizobium manausense strain BR 3351T, an effective symbiont isolated from Amazon rainforest
Provedor de dados: Brazilian Journal of Microbiology
Autores: Simões-Araújo,Jean Luiz; Rumjanek,Norma Gouvêa; Xavier,Gustavo Ribeiro; Zilli,Jerri Édson.
ABSTRACT The strain BR 3351T (Bradyrhizobium manausense) was obtained from nodules of cowpea (Vigna unguiculata L. Walp) growing in soil collected from Amazon rainforest. Furthermore, it was observed that the strain has high capacity to fix nitrogen symbiotically in symbioses with cowpea. We report here the draft genome sequence of strain BR 3351T. The information presented will be important for comparative analysis of nodulation and nitrogen fixation for diazotrophic bacteria. A draft genome with 9,145,311 bp and 62.9% of GC content was assembled in 127 scaffolds using 100 bp pair-end Illumina MiSeq system. The RAST annotation identified 8603 coding sequences, 51 RNAs genes, classified in 504 subsystems.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon forest; Biological nitrogen fixation; Next generation sequencing; Nodulation; Cowpea.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400610

Response of micropropagated sugarcane varieties to inoculation with endophytic diazotrophic bacteria
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira,André Luiz Martinez de; Canuto,Erineudo de Lima; Reis,Verônica Massena; Baldani,José Ivo.
Previous studies estimated that sugarcane could obtain up to 60% of total nitrogen accumulated from BNF. Here a mixture of five endophytic diazotrophic strains was tested in a field trial, inoculated in two micropropagated sugarcane varieties and three locals, to determine the effects on commercial crop conditions. The sugarcane plantlets were inoculated in vitro, and after 17 months of growing in the field, the productivity and BNF contribution showed to be influenced by the plant genotype and soil type. The highest BNF contributions was observed in the poorest soil for both varieties. Smaller increases in productivity were observed for SP 701143 variety grown in soil with low or medium fertility. In contrast, a decrease in the stem productivity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic bacteria inoculation; Biological nitrogen fixation; Plant growth promotion bacteria; Sugarcane.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500020

Draft genome sequence of Bradyrhizobium sp. strain BR 3262, an effective microsymbiont recommended for cowpea inoculation in Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Simões-Araújo,Jean Luiz; Leite,Jakson; Marie Rouws,Luc Felicianus; Passos,Samuel Ribeiro; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa; Zilli,Jerri Édson.
Abstract The strain BR 3262 was isolated from nodule of cowpea (Vigna unguiculata L. Walp) growing in soil of the Atlantic Forest area in Brazil and it is reported as an efficient nitrogen fixing bacterium associated to cowpea. Firstly, this strain was assigned as Bradyrhizobium elkanii, however, recently a more detailed genetic and molecular characterization has indicated it could be a Bradyrhizobium pachyrhizi species. We report here the draft genome sequence of B. pachyrhizi strain BR 3262, an elite bacterium used as inoculant for cowpea. The whole genome with 116 scaffolds, 8,965,178 bp and 63.8% of C+G content for BR 3262 was obtained using Illumina MiSeq sequencing technology. Annotation was added by the RAST prokaryotic genome annotation service and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Atlantic forest area; Biological nitrogen fixation; Next generation sequencing; Nodulation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400783

Nitrogen-fixing bacteria and arbuscular mycorrhizal fungi in Piptadenia gonoacantha (Mart.) Macbr.
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira Júnior,Joel Quintino de; Jesus,Ederson da Conceição; Lisboa,Francy Junio; Berbara,Ricardo Luis Louro; Faria,Sergio Miana de.
Abstract The family Leguminosae comprises approximately 20,000 species that mostly form symbioses with arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing bacteria (NFB). This study is aimed at investigating and confirming the dependence on nodulation and biological nitrogen fixation in the specie Piptadenia gonoacantha (Mart.) Macbr., which belongs to the Piptadenia group. Two consecutive experiments were performed in a greenhouse. The experiments were fully randomized with six replicates and a factorial scheme. For the treatments, the two AMF species and three NFB strains were combined to nodulate P. gonoacantha in addition to the control treatments. The results indicate this species’ capacity for nodulation without the AMF; however, the AMF + NFB...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synergy; Nodulation; Biological nitrogen fixation; “Pau-jacaré”.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100095

Multilocus sequence analysis (MLSA) of Bradyrhizobium strains: revealing high diversity of tropical diazotrophic symbiotic bacteria
Provedor de dados: Brazilian Journal of Microbiology
Autores: Delamuta,Jakeline Renata Marçon; Ribeiro,Renan Augusto; Menna,Pâmela; Bangel,Eliane Villamil; Hungria,Mariangela.
Symbiotic association of several genera of bacteria collectively called as rhizobia and plants belonging to the family Leguminosae (=Fabaceae) results in the process of biological nitrogen fixation, playing a key role in global N cycling, and also bringing relevant contributions to the agriculture. Bradyrhizobium is considered as the ancestral of all nitrogen-fixing rhizobial species, probably originated in the tropics. The genus encompasses a variety of diverse bacteria, but the diversity captured in the analysis of the 16S rRNA is often low. In this study, we analyzed twelve Bradyrhizobium strains selected from previous studies performed by our group for showing high genetic diversity in relation to the described species. In addition to the 16S rRNA,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Bradyrhizobium; Multilocus sequence analysis; Phylogeny; Taxonomy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200035

Genome sequence of Bradyrhizobium embrapense strain CNPSo 2833T, isolated from a root nodule of Desmodium heterocarpon
Provedor de dados: Brazilian Journal of Microbiology
Autores: Delamuta,Jakeline Renata Marçon; Ribeiro,Renan Augusto; Gomes,Douglas Fabiano; Souza,Renata Carolini; Chueire,Ligia Maria Oliveira; Hungria,Mariangela.
Abstract Bradyrhizobium embrapense CNPSo 2833T is a nitrogen-fixing symbiont of the legume pasture Desmodium. Its draft genome contains 8,267,832 bp and 7876 CDSs. The symbiotic island includes nodulation and nitrogen fixation genes resembling the operon organization of B. japonicum. Several CDSs related to secretion proteins and stress tolerance were also identified.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Genome; Nodulation; Symbiosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100009

Evaluation of the biological nitrogen fixation contribution in sugarcane plants originated from seeds and inoculated with nitrogen-fixing endophytes
Provedor de dados: Brazilian Journal of Microbiology
Autores: Canuto,Erineudo de Lima; Oliveira,André Luis Martinez de; Reis,Verônica Massena; Baldani,José Ivo.
The inoculation technique with endophytic diazotrophic bacteria in sugarcane has been shown as an alternative practice to plant growth promotion. The aim of this work was to evaluate the biological nitrogen fixation (BNF) contribution by different strains of Herbaspirillum seropedicae and Gluconacetobacter diazotrophicus in sugarcane plant inoculated from seeds. The seeds were planted in pots filled with non-sterile soil, inoculated with the bacterial strains and grown 10 months outside of the greenhouse. The BNF contribution of the inoculated bacteria varied depending on the plant species used as a control. The highest BNF contribution as well as the highest populations of reisolated bacteria was observed with inoculation of H. seropedicae strains. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic diazotrophic bacteria; Sugarcane; Biological nitrogen fixation; Herbaspirillum seropedicae; Gluconacetobacter diazotrophicus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500021

Triple inoculation with Bradyrhizobium, Glomus and Paenibacillus on cowpea (Vigna unguiculata [L.] walp.) development
Provedor de dados: Brazilian Journal of Microbiology
Autores: Lima,André Suêldo Tavares de; Xavier,Terezinha Ferreira; Lima,Cláudia Elizabete Pereira de; Oliveira,José de Paula; Mergulhão,Adália Cavalcanti do Espírito Santo; Figueiredo,Márcia do Vale Barreto.
The use of microorganisms to improve the availability of nutrients to plants is of great importance to agriculture. This study aimed to evaluate the effect of triple inoculation of cowpea with arbuscular mycorrhizal fungi (AMF), plant growth-promoting bacteria (PGPB) and rhizobia to maximize biological nitrogen fixation (BNF) and promote plant growth. The experiment was conducted in a greenhouse using cowpea plants (Vigna unguiculata L. Walp cv. IPA 206). The treatments included inoculation with strains of Bradyrhizobium sp. (BR 3267 and EI - 6) individually and as a mixture, an absolute control (AC) and mineral nitrogen control (NC), all combined with the presence or absence of native AMF (Glomus etunicatum) and PGPB (Paenibacillus brasilensis - 24) in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arbuscular mycorrhiza; Biological nitrogen fixation; PGPB; Rhizobia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300010

Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest
Provedor de dados: Brazilian Journal of Microbiology
Autores: Rodrigues,Dalila Ribeiro; Silva,Aleksandro Ferreira da; Cavalcanti,Maria Idaline Pessoa; Escobar,Indra Elena Costa; Fraiz,Ana Carla Resende; Ribeiro,Paula Rose de Almeida; Ferreira Neto,Reginaldo Alves; Freitas,Ana Dolores Santiago de; Fernandes-Júnior,Paulo Ivan.
Abstract Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; "Mulungu"; Diversity; Inoculant; 16S rRNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300503

Draft genome sequence of Bradyrhizobium sp. strain BR 3267, an elite strain recommended for cowpea inoculation in Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Simões-Araújo,Jean Luiz; Leite,Jakson; Passos,Samuel Ribeiro; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa; Zilli,Jerri Édson.
Abstract The strain BR 3267 is a nitrogen-fixing symbiotic bacteria isolated from soil of semi-arid area of Brazilian Northeast using cowpea as the trap plant. This strain is used as commercial inoculant for cowpea and presents high efficient in nitrogen fixation as consequence of its adaptation potential to semi-arid conditions. We report here the draft genome sequence of Bradyrhizobium sp. strain BR 3267, an elite bacterium used as inoculant for cowpea. Whole genome sequencing of BR 3267 using Illumina MiSeq sequencing technology has 55 scaffolds with a total genome size of 7,904,309 bp and C+G 63%. Annotation was added by the RAST prokaryotic genome annotation service and has shown 7314 coding sequences and 52 RNA genes.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Biological nitrogen fixation; Next generation sequencing; Nodulation; Semi-arid region.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400781

Common bean elite lines cultivated under nitrogen fertilization and inoculation with Rhizobium tropici
Provedor de dados: Ciência Rural
Autores: Pereira,Helton Santos; Melo,Leonardo Cunha; Faria,Luís Cláudio de; Ferreira,Enderson Petrônio de Brito; Mercante,Fábio Martins; Wendland,Adriane; Souza,Thiago Lívio Pessoa Oliveira de.
ABSTRACT: More efficient strains of Rhizobium have been selected for use in common bean. However, little effort was made with lines selection. The main goals of this research were to verify the presence of interaction involving common bean elite lines utilizing Nitrogen fertilization and Rhizobium inoculation for grain yield and to identify lines with superior yields utilizing biological nitrogen fixation. Eight field trials were conducted at four location-years in Brazilian savanna, using randomized complete blocks design with three replications. Each trial was composed of 17 carioca elite lines. Every two tests in each location were planted side by side, one with mineral nitrogen fertilization (90kg) and the other one with inoculation with Rhizobium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Biological nitrogen fixation; Grain yield; Genotype by environment interaction.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202168

Natural abundance of 15N of N derived from the atmosphere by different strains of Bradyrhizobium in symbiosis with soybean plants
Provedor de dados: Ciência Rural
Autores: Araujo,Karla Emanuelle Campos; Torres Júnior,Carlos Vergara; Guimarães,Ana Paula; Silva,Mara Alexandre da; Alves,Bruno José Rodrigues; Urquiaga,Segundo; Boddey,Robert Michael.
ABSTRACT:  To quantify the BNF contribution to legumes using the 15N natural abundance technique, it is important to know the abundance of 15N of the plants grown entirely dependent on BNF (value ‘B’). The aim of the study was to determine the 15N natural abundance of N2 fixed by different Bradyrhizobium strains in symbiosis with one soybean cultivar. Treatments consisted of soybean plants cultivated with and without inoculation with ten Bradyrhizobium strains, in five replicates planted in Leonard jars in a sand/vermiculite mixture. Plants were harvested after 46 days. The ‘B’ values of the aerial tissue (‘Bs’) ranged from -2.6 to -3.9 ‰. There was a tendency for the ‘Bs’ values of plants inoculated with strains of B. elkanii to be more negative than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 15N Isotopic fractionation; ‘B’ value; Biological nitrogen fixation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200251