Consulta: subjectFacets:"Biosynthesis"
Registros recuperados: 20
Data/hora: 04/06/2024 01:52:03

Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis
Provedor de dados: 69
Autores: Zhang,Wei-Wei; Yang,Ming-Ming; Li,Heng-xin; Wang,Dun.
As a precursor, pimelic acid plays an important role in biotin biosynthesis pathway of Bacillus subtilis. Fermentations supplemented with pimelic acid could improve the production of biotin, however, with a disadvantage-high cost. So it is necessary to improve the biosynthesis of pimelic acid via genetic engineering in B. subtilis. In this study, we constructed a recombinant B. subtilis strain for improving the synthesis of pimelic acid, in which a maltose-inducible Pglv promoter was inserted into the upstream of the cistron bioI-orf2-orf3 and, meanwhile, flanked by the tandem cistrons via a single crossover event. The copy number of the integrant was amplified by high-concentration resistance screen and increased to 4-5 copies. The production of pimelic...
Tipo: Journal article
Palavras-chave: Bacillus subtilis; Biosynthesis; Integration; Pglv promoter; Pimelic acid; Single crossover event.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600001

Genome-wide comparison of genes involved in the biosynthesis, metabolism, and signaling of juvenile hormone between silkworm and other insects
Provedor de dados: 74
Autores: Cheng,Daojun; Meng,Meng; Peng,Jian; Qian,Wenliang; Kang,Lixia; Xia,Qingyou.
Juvenile hormone (JH) contributes to the regulation of larval molting and metamorphosis in insects. Herein, we comprehensively identified 55 genes involved in JH biosynthesis, metabolism and signaling in the silkworm (Bombyx mori) as well as 35 in Drosophila melanogaster, 35 in Anopheles gambiae, 36 in Apis mellifera, 47 in Tribolium castaneum, and 44 in Danaus plexippus. Comparative analysis showed that each gene involved in the early steps of the mevalonate (MVA) pathway, in the neuropeptide regulation of JH biosynthesis, or in JH signaling is a single copy in B. mori and other surveyed insects, indicating that these JH-related pathways or steps are likely conserved in all surveyed insects. However, each gene participating in the isoprenoid branch of JH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juvenile hormone; Biosynthesis; Metabolism; Signaling; Gene duplication.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300018

Ecosystemes benthiques profonds et chimiosynthese bacterienne: Sources hydrothermales et suintements
Provedor de dados: 5
Autores: Laubier, Lucien.
Hydrothermal communities of various species compositions have been described from the eastern and western Pacific and Atlantic. Brine and cold seeps and hydrocarbon seepages have been found off Florida, off the Oregon and Japanese subduction systems, on the Laurentian Fan, near Barbados, and off Louisiana. Biomasses range from 10 to 70 kg/m super(2), fresh weight. Basic microdistribution of species groups in aureoles centered around hydrothermal vents reveals different levels of adaptation to harsh physico-chemical conditions. Two groups of primary producers can be recognized: a highly efficient cool water group with large vestimentiferan tube worms and bivalves, and alvinellid polychaetes adapted to hot waters. Both groups are exploited by specific...
Tipo: Text
Palavras-chave: Bivalvia; Polychaeta; Bacteria; Symbionts; Biosynthesis; Chemosynthesis; Food webs; Biomass; Hydrocarbons; Ecosystems; Hydrothermal springs; Seepages; Brines; Benthic environment; Benthos.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/acte-1171.pdf

Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus
Provedor de dados: 5
Autores: Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine.
Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the...
Tipo: Text
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf

Liaison de l'oestradiol 17 beta dans les hepatocytes de truite Arc-en-ciel et induction de la vitellogenine et de son ARNm specifique
Provedor de dados: 5
Autores: Maitre, J; Mercier, L; Dolo, L; Valotaire, Y.
In the liver of rainbow trout (Salmo gairdnerii ), vitellogenin (Vg) synthesis is influenced by oestradiol (E sub(2)) which is believed to act through the classical mechanism of steroid hormone action. After binding of the hormone to a soluble specific receptor protein, the oestradiol-receptor complex is translocated to the nucleus, where it interacts with DNA and modulates expression of Vg genes, leading to increased synthesis of specific mRNA and Vg. The authors show here: (i) the presence of specific oestrogen receptors in the cytosol of the male trout liver. (ii) The male liver, offering, an ideal experimental control of "zero" background, the authors followed - in the liver of male trouts - the kinetics of induction of Vg mRNA by hybridization with Vg...
Tipo: Text
Palavras-chave: Salmonidae; Pisces; Proteins; Liver; RNA; Steroids; Biosynthesis; Receptors; Sex hormones; Sexual reproduction.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1230.pdf

Exopolysaccharides produced by marine bacteria and their applications as glycosaminoglycan-like molecules
Provedor de dados: 5
Autores: Delbarre-ladrat, Christine; Sinquin, Corinne; Lebellenger, Lou; Zykwinska, Agata; Colliec-jouault, Sylvia.
Although polysaccharides are ubiquitous and the most abundant renewable bio-components, their studies, covered by the glycochemistry and glycobiology fields, remain a challenge due to their high molecular diversity and complexity. Polysaccharides are industrially used in food products; human therapeutics fall into a more recent research field and pharmaceutical industry is looking for more and more molecules with enhanced activities. Glycosaminoglycans (GAGs) found in animal tissues play a critical role in cellular physiological and pathological processes as they bind many cellular components. Therefore, they present a great potential for the design and preparation of therapeutic drugs. On the other hand, microorganisms producing exopolysaccharides (EPS)...
Tipo: Text
Palavras-chave: Marine-derivedpolysaccharide; Biodiversity; Biologicalactivity; Structure; Modification; GAG-mimetic; Biosynthesis.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00241/35250/33764.pdf

Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae
Provedor de dados: 5
Autores: Lebellenger, Lou; Verrez-bagnis, Veronique; Passerini, Delphine; Delbarre Ladrat, Christine.
Objectives The eps locus in Vibrio diabolicus is involved in the production of the biotechnologically valuable HE800 EPS. In this study, the distribution and diversity of similar eps gene clusters across Vibrionaceae and its variability in relation to phylogenetic relationship were investigated. The aim was to provide a better knowledge of the eps gene cluster importance and to facilitate discovery of new EPS with potent interesting bioactivities. Results Seventy percent of the 103 genome sequences examined display such an eps locus with a high level of synteny. However, genetic divergence was found inside some monophyletic clades or even between some strains of the same species. It includes gene insertions, truncations, and deletions. Comparative analysis...
Tipo: Text
Palavras-chave: Vibrionaceae; Exopolysaccharide; Genetic biodiversity; Biosynthesis; Vibrio diabolicus; HE800 EPS.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00424/53536/54392.pdf

Chemotypes and Biomarkers of Seven Species of New Caledonian Liverworts from the Bazzanioideae Subfamily
Provedor de dados: 5
Autores: Metoyer, Benjamin; Lebouvier, Nicolas; Hnawia, Edouard; Herbette, Gaetan; Thouvenot, Louis; Asakawa, Yoshinori; Nour, Mohammed; Raharivelomanana, Phila.
Volatile components of seven species of the Bazzanioideae sub-family (Lepidoziaceae) native to New Caledonia, including three endemic species (Bazzania marginata, Acromastigum caledonicum and A. tenax), were analyzed by GC-FID-MS in order to index these plants to known or new chemotypes. Detected volatile constituents in studied species were constituted mainly by sesquiterpene, as well as diterpene compounds. All so-established compositions cannot successfully index some of them to known chemotypes but afforded the discovery of new chemotypes such as cuparane/fusicoccane. The major component of B. francana was isolated and characterized as a new zierane-type sesquiterpene called ziera-12(13), 10(14)-dien-5-ol (23). In addition, qualitative intraspecies...
Tipo: Text
Palavras-chave: Liverwort; Bazzania; Acromastigum; Sesquiterpene; Diterpene; Bis(bibenzyl); Biosynthesis; Zierane; Vittatin.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00660/77222/78670.pdf

CHICKPEA BIBLIOGRAPHY
Provedor de dados: 30
Autores: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT).
Palavras-chave: Chickpeas; Grain legumes; Amino acids; Peas; Proteins; Enzymes; Sowing rates; Vitamins; Wheats; Biosynthesis.
Ano: 1977
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3940

Bioassay for detection of transgenic soybean seeds tolerant to glyphosate
Provedor de dados: 86
Autores: Torres,Antonio Carlos; Nascimento,Warley Marco; Paiva,Sonia Alessandra Vasconcelos; Aragão,Fernando Antonio Souza de.
Glyphosate is a systemic, nonselective, postemergence herbicide that inhibits growth of both weeds and crop plants. Once inside the plant, glyphosate interferes with biosynthesis of aromatic amino acids phenylalanine, tyrosine, and tryptophan, by inhibiting the activity of 5enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway. The objective of this work was to develop a simple, effective and inexpensible method for identification of transgenic soybean tolerant to glyphosate. This technique consisted in germinating soybean seeds in filter paper moistened with 100 to 200 muM of glyphosate. Transgenic soybean seeds tolerant to glyphosate germinated normally in this solution and, between 7 and 10 days, started to develop a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Herbicide resistance; Transgenic plants; Biosynthesis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000900005

Abscisic acid biosynthesis and catabolism and their regulation roles in fruit ripening
Provedor de dados: 85
Autores: Yang,FW; Feng,XQ.
Abscisic acid (ABA) plays a series of significant physiology roles in higher plants including but not limited to promote bud and seed dormancy, accelerate foliage fall, induce stomatal closure, inhibit growth and enhance resistance. Recently, it has been revealed that ABA also has an important regulator role in the growth, development and ripening of fruit. In higher plants ABA is produced from an indirect pathway from the cleavage products of carotenoids. The accumulation of endogenous ABA levels in plants is a dynamic balance controlled by the processes of biosynthesis and catabolism, through the regulation of key ABA biosynthetic gene and enzyme activities. It has been hypothesized that ABA levels could be part of the signal that trigger fruit ripening,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abscisic acid (ABA); Biosynthesis; Catabolism; Regulation; Fruit; Ripening.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000200025

Researching the possibilities of the pectolytic ensyme biosynthesis with the aspergillus species of microscopic fungi
Provedor de dados: 31
Autores: Mojsov,   Kiro.
For the purpose of acquiring a highly active producer of pectolytic enzymes, there was a probe of isolating 140 species of Aspergillus’s fungi from different substrates (soils, grape malts, grapes, apples and sugar beet shreds). The isolated kinds of fungi were kept on a slant agar surface according to Chapek, with 2% of pectin. Testing was performed on the isolated layers of fungi as to the production of pectinolytic enzymes. The nourishing base used was  the synthetic Chapek base with 2% pectin,  2% lactose and 0.7 % (NH4)2HPO4, and a natural base  of 1% refuse apple pulp. Within 48 hours after cultivating the fungi, the filtrates were tested by the viscozimetric method to determine their entire pectolytic activity. The acquired results showed that a...
Tipo: Journal Article
Palavras-chave: Biosynthesis; Pectolytic enzymes; Apple pulp; Aspergillus.; Land Economics/Use; L65.
Ano: 2009
URL: http://purl.umn.edu/94608

Biologically Synthesized Silver Nanoparticles for Enhancing Tetracycline Activity Against Staphylococcus aureus and Klebsiella pneumoniae
Provedor de dados: 52
Autores: Hussein,Elsayed Alsaied Masoud; Mohammad,Ali Al-Hajry; Harraz,Farid Abourageh; Ahsan,Mohd Faisal.
Abstract Phytochemical content of plant extracts can be used effectively to reduce the metal ions to nanoparticles in one-step green synthesis process. In this study, six plant extracts were used for the synthesis of silver nanoparticles (AgNPs). Biologically synthesized AgNPs was characterized using UV-Vis Spectrophotometer, Field Emission Scanning Electron Microscope (FE-SEM), X-ray diffraction (XRD), Energy Dispersive X-ray spectroscopy (EDX) and Fourier Transform Infrared (FTIR) spectroscopy. The individual and combined effects of AgNPs and tetracycline against S. aureus and K. pneumoniae were assessed. Ginger, onion and sidr extracts supported AgNPs formation while arak, garlic and mint extracts failed to convert the silver ions to AgNPs. The present...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver nanoparticles; Biosynthesis; Plant extracts; Tetracycline; S. aureus; K. pneumoniae.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100308

Microbial Synthesis and Characterization of Biodegradable Polyester Copolymers from Burkholderia Cepacia and Cupriavidus Necator Strains Using Crude Glycerol as Substrate
Provedor de dados: 52
Autores: Rodrigues,Plínio Ribeiro; Silvério,Tatiane Aparecida Barroso; Druzian,Janice Izabel.
Abstract Polymers are materials of pronounced importance in the modern world, since they are massively present in everyday life, especially in the form of packaging. However, most of these materials are derived from non-renewable sources and their disposal generates large volumes of waste, which is extremely damaging to natural environments. In this context, microbial biopolymers appear as a powerful alternative in the substitution of several applications of synthetic plastics, causing less harm to the environment, as they are biodegradable and produced from renewable sources. In this study, evaluation and characterization of polyhydroxyalkanoates (PHAs) produced by Cupriavidus necator (IPT 026 and IPT 027) and Burkholderia cepacia (IPT 119 and IPT 400),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crude glycerol; Biosynthesis; Polyhydroxyalkanoates; Characterization.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100209

Heparan sulphate, its derivatives and analogues share structural characteristics that can be exploited, particularly in inhibiting microbial attachment
Provedor de dados: 56
Autores: Rudd,T.R.; Hughes,A.; Holman,J.; Solari,V.; de Oliveira Ferreira,E.; Cavalcante Pilotto Domingues,R.M.; Yates,E.A.
Heparan sulphate (HS) and the related polysaccharide, heparin, exhibit conformational and charge arrangement properties, which provide a degree of redundancy allowing several seemingly distinct sequences to exhibit the same activity. This can also be mimicked by other sulphated polysaccharides, both in overall effect and in the details of interactions and structural consequences of interactions with proteins. Together, these provide a source of active compounds suitable for further development as potential drugs. These polysaccharides also possess considerable size, which bestows upon them an additional useful property: the capability of disrupting processes comprising many individual interactions, such as those characterising the attachment of microbial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparan sulfate; Biosynthesis; Structure-function; Microbial attachment.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500002

Expression of phenazine biosynthetic genes during the arbuscular mycorrhizal symbiosis of Glomus intraradices
Provedor de dados: 58
Autores: León-Martínez,Dionicia Gloria; Vielle-Calzada,Jean-Philippe; Olalde-Portugal,Víctor.
To explore the molecular mechanisms that prevail during the establishment of the arbuscular mycorrhiza symbiosis involving the genus Glomus, we transcriptionally analysed spores of Glomus intraradices BE3 during early hyphal growth. Among 458 transcripts initially identified as being expressed at presymbiotic stages, 20% of sequences had homology to previously characterized eukaryotic genes, 30% were homologous to fungal coding sequences, and 9% showed homology to previously characterized bacterial genes. Among them, GintPbr1a encodes a homolog to Phenazine Biosynthesis Regulator (Pbr) of Burkholderia cenocepacia, an pleiotropic regulatory protein that activates phenazine production through transcriptional activation of the protein D isochorismatase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycorrhizal fungi; Glomus intradices; Phenazine; Biosynthesis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200037

Effect of branched-chain amino acids, valine, isoleucine and leucine on the biosythesis of bitespiramycin 4"-O-acylspiramycins
Provedor de dados: 58
Autores: Li,Zhen-Lin; Wang,Yong-Hong; Chu,Ju; Zhuang,Ying-Ping; Zhang,Si-Liang.
Bitespiramycin, a group of 4"-O-acylated spiramycins with 4"-O-isovalerylspiramycins as the major components, was produced by recombinantspiramycin-producing strain Streptomyces spiramyceticus harboring a 4"-O-acyltransferase gene. The experiment was initially performed in synthetic medium with 0.5 g l-1 Valine, Isoleucine or Leucine feeding at 36 h cultivation. When valine was fed, the biological titer of bitespiramycin was 45.3% higher than that of the control group, but the relative content of total isovalerylspiramycin components decreased by 22.5%. In the case of ilecine, the biological titer of bitespiramycin and the total isovalerylspiramycins alone were 85% and 72.1% of the control group, respectively. In contrast, the relative content of other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bitespiramycin; Valine; Leucine; Isoleucine; Biosynthesis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400003

Antibacterial properties of silver nanoparticles synthesized by marine Ochrobactrum sp.
Provedor de dados: 58
Autores: Thomas,Roshmi; Janardhanan,Anju; Varghese,Rintu T.; Soniya,E.V.; Mathew,Jyothis; Radhakrishnan,E.K.
Metal nanoparticle synthesis is an interesting area in nanotechnology due to their remarkable optical, magnetic, electrical, catalytic and biomedical properties, but there needs to develop clean, non-toxic and environmental friendly methods for the synthesis and assembly of nanoparticles. Biological agents in the form of microbes have emerged up as efficient candidates for nanoparticle synthesis due to their extreme versatility to synthesize diverse nanoparticles with varying size and shape. In the present study, an eco favorable method for the biosynthesis of silver nanoparticles using marine bacterial isolate has been attempted. Very interestingly, molecular identification proved it as a strain of Ochrobactrum anhtropi. In addition, the isolate was found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosynthesis; Silver nanoparticles; Ochrobactrum anhtropi; Purification; Antibacterial activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400012

Microbial mediated preparation, characterization and optimization of gold nanoparticles
Provedor de dados: 58
Autores: Barabadi,Hamed; Honary,Soheila; Ebrahimi,Pouneh; Mohammadi,Milad Ali; Alizadeh,Ahad; Naghibi,Farzaneh.
The need for eco-friendly and cost effective methods for nanoparticles synthesis is developing interest in biological approaches which are free from the use of toxic chemicals as byproducts. This study aimed to biosynthesize and optimize the size of gold nanoparticles which produced by biotechnological method using Penicillium crustosum isolated from soil. Initially, Penicillium crustosum was grown in fluid czapek dox broth on shaker at 28 ºC and 200 rpm for ten days and then the supernatant was separated from the mycelia to convert AuCl4 solution into gold nanoparticles. The synthesized nanoparticles in the optimum conditions were formed with fairly well-defined dimensions and good monodispersity. The characterizations were done by using different methods...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosynthesis; Optimization; Gold nanoparticles; Box-behnken design; Penicillium crustosum.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400046

Metabolic pathway of oligosaccharides in sorghum honeydew caused by Claviceps africana
Provedor de dados: 65
Autores: Bogo,Amauri; Mantle,Peter.
The occurrence of the alditol oligosaccharides in the Claviceps afriana honeydew is partly as a rational expression of the pathogen's selective nutritive metabolism of the sucrose supplied by the host plant. The experiments were carried out in laboratory and when 14C-D-sucrose, 14C-D-fructose or 14C-D-mannitol radiolabelled saccharides were incorporated into: a) sorghum plant infected by C. africana, b) whole and macereted micelia tissue and c) cell-free honeydew of C. africana, it was observed that the glucose moiety of sucrose was not involved in oligosaccharides formation. Glucose was used by the pathogen as nutritional source. Part of the unused fructose moiety was reduced to mannitol by the pathogen's enzymes which was also excreted into honeydew...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosynthesis; Metabolic pathway; Oligosaccharides; Ergot.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400008