Consulta: subjectFacets:"Bone marrow"
Registros recuperados: 16
Data/hora: 04/06/2024 02:33:00

Mesenchymal stem cell therapy promotes the improvement and recovery of renal function in a preclinical model
Provedor de dados: 74
Autores: Urt-Filho,Antônio; Oliveira,Rodrigo Juliano; Hermeto,Larissa Correa; Pesarini,João Renato; David,Natan de; Cantero,Wilson de Barros; Falcão,Gustavo; Marks,Guido; Antoniolli-Silva,Andréia Conceição Milan Brochado.
Abstract Acute renal failure (ARF) is an extremely important public health issue in need of novel therapies. The present study aimed to evaluate the capacity of mesenchymal stem cell (MSC) therapy to promote the improvement and recovery of renal function in a preclinical model. Wistar rats were used as the experimental model, and our results show that cisplatin (5mg/kg) can efficiently induce ARF, as measured by changes in biochemical (urea and creatinine) and histological parameters. MSC therapy performed 24h after the administration of chemotherapy resulted in normalized plasma urea and creatinine levels 30 and 45d after the onset of kidney disease. Furthermore, MSC therapy significantly reduced histological changes (intratubular cast formation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow; Chemotherapy; Nephrotoxicity; Kidney disease; Tissue regeneration.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200290

Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay
Provedor de dados: 74
Autores: Boriollo,Marcelo Fabiano Gomes; Resende,Marielly Reis; Silva,Thaísla Andrielle da; Públio,Juliana Yoshida; Souza,Luiz Silva; Dias,Carlos Tadeu dos Santos; Oliveira,Nelma de Mello Silva; Fiorini,João Evangelista.
The aim of this study was to evaluate the mutagenicity (clastogenicity/aneugenicity) of a glycolic extract of Ziziphus joazeiro bark (GEZJ) by the micronucleus assay in mice bone marrow. Antimutagenic activity was also assessed using treatments associated with GEZJ and doxorubicin (DXR). Mice were evaluated 24-48 h after exposure to positive (N-nitroso-N-ethylurea, NEU - 50 mg.kg-1 and DXR - 5 mg.kg-1) and negative (150 mM NaCl) controls, as well as treatment with GEZJ (0.5-2 g.kg-1), GEZJ (2 g.kg-1) + NEU and GEZJ (2 g.kg-1) + DXR. There were no significant differences in the frequencies of micronucleated polychromatic erythrocytes in mice treated with GEJZ and GEJZ + DXR compared to the negative controls, indicating that GEZJ was not mutagenic. Analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimutagenicity; Bone marrow; Doxorubicin; Micronucleus assay; Mutagenicity; Zizyphus joazeiro Mart. (raspa-de-Juá).
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300016

Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
Provedor de dados: 74
Autores: Del Valle,Paulo Roberto; Milani,Cintia; Brentani,Maria Mitzi; Katayama,Maria Lucia Hirata; Lyra,Eduardo Carneiro de; Carraro,Dirce Maria; Brentani,Helena; Puga,Renato; Lima,Leandro A.; Rozenchan,Patricia Bortman; Nunes,Bárbara dos Santos; Góes,João Carlos Guedes Sampaio; Folgueira,Maria Aparecida Azevedo Koike.
Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR &lt; 0.01). Functional analysis revealed that genes related to development and morphogenesis were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow; Breast neoplasm; Fibroblast; Gene expression profiling; Lymph node; Mesenchymal stromal cells; Tumor microenvironment.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002

Ex vivo study of hematopoiesis in protein malnutrition: characterization of bone marrow stromal cells in mice
Provedor de dados: 195
Autores: Santos, Ed Wilson; Dias, Carolina; Oliveira, Dalila Cunha de; Hastreiter, Araceli; Silva, Graziela Batista da; Beltran, Jackeline Soares de Oliveira; Paredes-Gamero, Edgar Julian; Borelli, Primavera.
Protein Malnutrition (PM) can promotes the modification of hematopoietic tissue, with changes of extracellular matrix components, affecting the evolution of the cell cycle of hemopoietic cells, reducing the production of precursors and hematopoietic cells both in erythroid series and in the number of leukocytes, leading to anemia and leukopenia. In view of these events, it was hypothetised that changes can occur in the formation and establishment of bone marrow stroma, as well as in cells proliferation, differentiation and maturation, resulting in the change in the microenvironment inducing hemopoese, affecting signaling cellular processes and the physiology of stem and progenitor cells
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoiesis; Protein malnutrition; Bone marrow; Stromal cells; Mice hematopoiesis; Protein malnutrition; Bone marrow; Stromal cells; Mice.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31114

Gap junctions in hematopoietic stroma control proliferation and differentiation of blood cell precursors
Provedor de dados: 42
Autores: Bodi,Estevão; Hurtado,Sandra P.; Carvalho,Marcelo A.; Borojevic,Radovan; Carvalho,Antônio C. Campos de.
We examined gap junction communication in an in vitro model of hematopoiesis, using the murine bone marrow stroma cell line S-17, and primary cultures of murine marrow-derived blood cell precursors. S-17 cells express several connexins, the major one being connexin 43. Connexin expression and formation of functional gap junctions is modulated by stroma cell density. Transfection of S-17 cells with a vector containing connexin 43 sense or anti-sense sequences increased or decreased, respectively, connexin 43 synthesis and intercellular dye coupling. Under these conditions, modulation of gap junction-mediated communication modified the growth pattern of stroma itself, as well as the ability of the stroma to sustain hematopoiesis. Increased connexin 43...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow; Hematopoiesis; Connexins; Gap junctions; S-17 cells.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400009

Behavior of mesenchymal stem cells stained with 4&amp;', 6-diamidino-2-phenylindole dihydrochloride (DAPI) in osteogenic and non osteogenic cultures
Provedor de dados: 47
Autores: Ocarino,N.M.; Bozzi,A.; Pereira,R.D.O.; Breyner,N.M.; Silva,V.L.; Castanheira,P.; Goes,A.M.; Serakides,R.
4&amp;', 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them to neighboring cells, while DAPI-treated cells were mostly round cells with fine and short extensions....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow; Cell cultures; Rat; Osteogenic differentiation.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200003

Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate
Provedor de dados: 47
Autores: Barrios,Lilian; Poletti,Oscar Héctor.
We have evaluated the effect of filgrastim on proliferation and differentiation activity of granulopoietic cells in mice pretreated with methotrexate. Filgrastim was injected daily, from day 8 to 28 after cytotoxic agent administration. The granulopoiesis changes were measured by assessment of GM-CFU cells content, marrow and spleen granuloid cells pool as well as circulating neutrophils. In MTX pretreated mice, bone marrow GM-CFU oscillating values were higher than normal values, but these changes were not followed by high proliferative activity in granuloid precursor cell compartment. After MTX treatment, filgrastim administration was unable to stimulate marrow granulopoiesis as observed in normal mice. In the spleen, MTX led to dramatic changes in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methotrexate; Filgrastim; Granulopoiesis; Bone marrow; Spleen.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100002

Stem cells and respiratory diseases
Provedor de dados: 52
Autores: Abreu,Soraia Carvalho; Maron-Gutierrez,Tatiana; Garcia,Cristiane Sousa Nascimento Baez; Morales,Marcelo Marcos; Rocco,Patricia Rieken Macedo.
Stem cells have a multitude of clinical implications in the lung. This article is a critical review that includes clinical and experimental studies of MedLine and SciElo database in the last 10 years, where we highlight the effects of stem cell therapy in acute respiratory distress syndrome or more chronic disorders such as lung fibrosis and emphysema. Although, many studies have shown the beneficial effects of stem cells in lung development, repair and remodeling; some important questions need to be answered to better understand the mechanisms that control cell division and differentiation, therefore enabling the use of cell therapy in human respiratory diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow; Fibrosis; Repair; Acute respiratory distress syndrome; Asthma; Emphysema.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700005

Genotoxic activity of the insecticide Nuvacron (Monocrotophos) detected by the micronucleus test in bone marrow erythrocytes of mice and in CHO cells
Provedor de dados: 54
Autores: Peitl Jr,Paulo; Sakamoto-Nojo,Elza T.; Cólus,Ilce M. de Syllos.
The organophosphorus insecticide Nuvacron (Monocrotophos) is a very toxic agent widely utilized in Brazilian agriculture. To evaluate the clastogenic potential of this insecticide, in vivo and in vitro micronucleus (MN) assay experiments were carried out on Swiss mice and on Chinese hamster ovary (CHO) cells, respectively. Nuvacron administered at doses of 2.5 and 5.0 mg/kg induced a statistically significant increase in the frequencies of MN detected in polychromatic bone marrow erythrocytes from animals (six/group) treated ip 24 h before. Exponentially growing CHAO cells were treated continuously (16h) with Nuvacron diluted in water to final concentrations of 1, 10, 100, 200, and 400 mug/ml. Three experiments were carried out using the cytokinesis-block...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxic; Insecticide Nuvacron; Micronucleus; Bone marrow; Erythrocytes; Mice; CHO cells.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400006

Pharmacokinetic and parasitological evaluation of the bone marrow of dogs with visceral leishmaniasis submitted to multiple dose treatment with liposome-encapsulated meglumine antimoniate
Provedor de dados: 56
Autores: Schettini,D.A.; Costa Val,A.P.; Souza,L.F.; Demicheli,C.; Rocha,O.G.F.; Melo,M.N.; Michalick,M.S.M.; Frézard,F.
The aim of the present study was to evaluate the impact of a multiple dose regimen of a liposomal formulation of meglumine antimoniate (LMA) on the pharmacokinetics of antimony in the bone marrow of dogs with visceral leishmaniasis and on the ability of LMA to eliminate parasites from this tissue. Dogs naturally infected with Leishmania chagasi received 4 intravenous doses of either LMA (6.5 mg antimony/kg body weight, N = 9), or empty liposomes (at the same lipid dose as LMA, N = 9) at 4-day intervals. A third group of animals was untreated (N = 8). Before each administration and at different times after treatment, bone marrow was obtained and analyzed for antimony level (LMA group) by electrothermal atomic absorption spectrometry, and for the presence of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Liposome; Meglumine antimoniate; Pharmacokinetics; Leishmaniasis; Dogs; Bone marrow.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200017

Protective effects of deferasirox and N-acetyl-L-cysteine on iron overload-injured bone marrow
Provedor de dados: 56
Autores: Shen,J.C.; Zhang,Y.C.; Zhao,M.F.
Using an iron overload mouse model, we explored the protective effect of deferasirox (DFX) and N-acetyl-L-cysteine (NAC) on injured bone marrow hematopoietic stem/progenitor cells (HSPC) induced by iron overload. Mice were intraperitoneally injected with 25 mg iron dextran every 3 days for 4 weeks to establish an iron overload (Fe) model. DFX or NAC were co-administered with iron dextran in two groups of mice (Fe+DFX and Fe+NAC), and the function of HSPCs was then examined. Iron overload markedly decreased the number of murine HSPCs in bone marrow. Subsequent colony-forming cell assays showed that iron overload also decreased the colony forming capacity of HSPCs, the effect of which could be reversed by DFX and NAC. The bone marrow hematopoiesis damage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron overload; Bone marrow; Hematopoietic stem/progenitor cells; Deferasirox; N-acetyl-L-cysteine.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200613

The role of osteoblasts in regulating hematopoietic stem cell activity and tumor metastasis
Provedor de dados: 56
Autores: Neiva,K.; Sun,Y.-X.; Taichman,R.S.
Bone marrow stromal cells are critical regulators of hematopoiesis. Osteoblasts are part of the stromal cell support system in bone marrow and may be derived from a common precursor. Several studies suggested that osteoblasts regulate hematopoiesis, yet the entire mechanism is not understood. It is clear, however, that both hematopoietic precursors and osteoblasts interact for the production of osteoclasts and the activation of resorption. We observed that hematopoietic stem cells (HSCs) regulate osteoblastic secretion of various growth factors, and that osteoblasts express some soluble factors exclusively in the presence of HSCs. Osteoblasts and hematopoietic cells are closely associated with each other in the bone marrow, suggesting a reciprocal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoblasts; Hematopoietic stem cells; Bone marrow; Stromal-derived factor-1; CXCR4; Prostate cancer.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000001

Alterations in proteins of bone marrow extracellular matrix in undernourished mice
Provedor de dados: 56
Autores: Vituri,C.L.; Alvarez-Silva,M.; Trentin,A.G.; Borelli,P.
The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4% casein as compared to 20% casein in the control diet. When the experimental group had attained a 20% loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5%) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibronectin; Bone marrow; Extracellular matrix; Malnutrition.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800004

Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration
Provedor de dados: 56
Autores: Rossi,M.I.D.; Barros,A.P.D.N.; Baptista,L.S.; Garzoni,L.R.; Meirelles,M.N.; Takiya,C.M.; Pascarelli,B.M.O.; Dutra,H.S.; Borojevic,R.
Cell fate decisions are governed by a complex interplay between cell-autonomous signals and stimuli from the surrounding tissue. In vivo cells are connected to their neighbors and to the extracellular matrix forming a complex three-dimensional (3-D) microenvironment that is not reproduced in conventional in vitro systems. A large body of evidence indicates that mechanical tension applied to the cytoskeleton controls cell proliferation, differentiation and migration, suggesting that 3-D in vitro culture systems that mimic the in vivo situation would reveal biological subtleties. In hematopoietic tissues, the microenvironment plays a crucial role in stem and progenitor cell survival, differentiation, proliferation, and migration. In adults, hematopoiesis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multicellular spheroid; Three-dimensional culture; Cell migration; Bone marrow.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000002

Impairment of the hematological response and interleukin-1β production in protein-energy malnourished mice after endotoxemia with lipopolysaccharide
Provedor de dados: 56
Autores: Fock,R.A.; Vinolo,M.A.R.; Blatt,S.L.; Borelli,P.
The objectives of this study were to determine if protein-energy malnutrition (PEM) could affect the hematologic response to lipopolysaccharide (LPS), the interleukin-1β (IL-1β) production, leukocyte migration, and blood leukocyte expression of CD11a/CD18. Two-month-old male Swiss mice were submitted to PEM (N = 30) with a low-protein diet (14 days) containing 4% protein, compared to 20% protein in the control group (N = 30). The total cellularity of blood, bone marrow, spleen, and bronchoalveolar lavage evaluated after the LPS stimulus indicated reduced number of total cells in all compartments studied and different kinetics of migration in malnourished animals. The in vitro migration assay showed reduced capacity of migration after the LPS stimulus in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood; Bone marrow; Spleen; Interleukin-1β; Lipopolysaccharide; Malnutrition.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200009

Linking immunity and hematopoiesis by bone marrow T cell activity
Provedor de dados: 56
Autores: Monteiro,J.P.; Bonomo,A.
Two different levels of control for bone marrow hematopoiesis are believed to exist. On the one hand, normal blood cell distribution is believed to be maintained in healthy subjects by an "innate" hematopoietic activity, i.e., a basal intrinsic bone marrow activity. On the other hand, an "adaptive" hematopoietic state develops in response to stress-induced stimulation. This adaptive hematopoiesis targets specific lineage amplification depending on the nature of the stimuli. Unexpectedly, recent data have shown that what we call "normal hematopoiesis" is a stress-induced state maintained by activated bone marrow CD4+ T cells. This T cell population includes a large number of recently stimulated cells in normal mice whose priming requires the presence of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T cell; Hematopoiesis; Innate immunity; Adaptive immunity; Bone marrow; Immunological memory.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000004