Consulta: subjectFacets:"Bulk segregant analysis"
Registros recuperados: 3
Data/hora: 09/06/2024 04:27:40

Identification of SSR and RAPD markers linked to a resistance allele for angular leaf spot in the common bean (Phaseolus vulgaris) line ESAL 550
Provedor de dados: 74
Autores: Silva,Gilvan Ferreira da; Santos,João Bosco dos; Ramalho,Magno Antonio Patto.
The objective of this study was to identify RAPD and SSR markers associated with a resistant allele for angular leaf spot (Phaeoisariopsis griseola) from the line 'ESAL 550', derived from the Andean 'Jalo EEP 558' cultivar, to assist selection of resistant genotypes. The resistant line 'ESAL 550' and the susceptible cultivar 'Carioca MG' were crossed to generate F1 and F2 populations. One hundred and twenty F2:3 families were evaluated. The DNA of the 12 most resistant families was bulked and the same was done with the DNA of the 10 most susceptible, generating two contrasting bulks. One RAPD and one SSR marker was found to be linked in coupling phase to the resistant allele. The SSR marker was amplified by the primer PV-atct001(282C), and its distance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Phaeoisariopsis griseola; DNA markers; Resistance; Bulk segregant analysis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400009

Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
Provedor de dados: 63
Autores: Agapito-Tenfen,Sarah Zanon; Dantas,Adriana Cibele de Mesquita; Denardi,Frederico; Nodari,Rubens Onofre.
Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) is a major insect pest that has significant economic impact on apple growers worldwide. Modern breeding technologies rely on several molecular tools to help breeders select genetic determinants for traits of interest. Consequently, there is a need for specific markers linked to the genes of interest. Apple scions and rootstocks have an additional barrier to the introduction of pest resistance genes due to the presence of self-incompatibility S-RNase alleles. The genetic characterization and early identification of these alleles can amplify the contribution of a breeding program to the selection of resistant genitors that are as compatible as possible. In this study, we identified the Er1 gene involved in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malus pumila; Woolly apple aphid; Self-incompatibility; Bulk segregant analysis; Marker-assisted selection.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100062

BSA and molecular markers screening for salt stress tolerant mutant of Petunia obtained in in vitro culture
Provedor de dados: 65
Autores: Krupa-Małkiewicz,Marcelina; Bienias,Anna.
ABSTRACT: In this study, we performed BSA to identify genetic markers linked to salt tolerance. We tested the genetic diversity among four bulked DNA samples of EMS induced mutant clones and one bulked DNA sample of non-mutated clone of Petunia for salt tolerance in in vitro callus cultures using RAPD and ISSR markers. Out of the 36 RAPD and 16 ISSR primers identified, 25 and 13 were effectively used to amplify genomic DNA of all the five bulked samples, respectively. In total, 114 RAPD amplifications products were obtained, of which 28% were polymorphic and 2% were genotype-specific bands. Out of the 64 ISSR amplification products obtained, 51% were polymorphic and 1% was genotype-specific bands. Results of this study indicated the existence of two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bulk segregant analysis; Chemical mutagenesis; ISSR; RAPD; Petunia × atkinsiana D. Don; Salt stress; Tissue culture.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200400