Consulta: subjectFacets:"Burkitt lymphoma"
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Data/hora: 10/06/2024 03:36:18

FAMLF is a target of miR-181b in Burkitt lymphoma
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Li,J.G.; Ding,Y.; Huang,Y.M.; Chen,W.L.; Pan,L.L.; Li,Y.; Chen,X.L.; Chen,Y.; Wang,S.Y.; Wu,X.N.
Burkitt lymphoma (BL) is a highly malignant non-Hodgkin's lymphoma that is closely related to the abnormal expression of genes. Familial acute myelogenous leukemia related factor (FAMLF; GenBank accession No. EF413001.1) is a novel gene that was cloned by our research group, and miR-181b is located in the intron of the FAMLF gene. To verify the role of miR-181b and FAMLF in BL, RNAhybrid software was used to predict target site of miR-181b on FAMLF and real-time quantitative PCR (RQ-PCR) was used to detect expression of miR-181b and FAMLF in BL patients, Raji cells and unaffected individuals. miR-181b was then transfected into Raji and CA46 cell lines and FAMLF expression was examined by RQ-PCR and western blotting. Further, Raji cells viability and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Burkitt lymphoma; FAMLF; MiR-181b; Gene expression; RQ-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600701