Consulta: subjectFacets:"CREB"
Registros recuperados: 5
Data/hora: 08/06/2024 13:30:49

IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
Provedor de dados: 48
Autores: JAIMOVICH,ENRIQUE; CARRASCO,MARIA ANGELICA.
Potassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave along the myotube showing a distinct calcium transient at the level of cell nuclei. Due to the presence of IP3 receptors both in the SR (A-band region) and in the nuclear envelope, these two events appear to be related; iii) Phosphorylation of mitogen activated kinases (ERK 1/2) and of the transcription factor CREB (30 s-10 min), as well as expression of the early genes c-fos, c-jun and egr-1 mRNA (5-15 min). Several independent pieces of evidence, including...
Tipo: Journal article
Palavras-chave: Excitation-transcription; Inositol trisphosphate; Mitogen-activated kinases; CREB; Early genes; Dihydropyridine receptors.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200010

Systemic injection of an H4 receptor agonist induces a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex in mice
Provedor de dados: 56
Autores: Fernandes,C.E.M.; Serafim,K.R.; Gianlorenço,A.C.L.; Mattioli,R.
Studies have shown that an injection with the histamine H4 receptor agonist VUF-8430 modulates emotional memory processes. In the present study, the aim was to verify if intraperitoneal (ip) injection of VUF-8430 (500 ng/kg) in mice affects the synthesis of proteins required for memory consolidation processes by activating the phosphorylation of CREB (pCREB) in classical structures linked to emotional memory (prefrontal cortex, amygdala, and hippocampus) and the cerebellar vermis, a structure that has also been recently implicated in emotional memory. The results obtained using western blot analysis demonstrated that VUF-8430 induced a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex, suggesting that this dose impaired the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CREB; PCREB; VUF-8430; Western blotting; Emotional memory; Mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500603

Cardioprotection against experimental myocardial ischemic injury using cornin
Provedor de dados: 56
Autores: Xu,Y.; Xu,Y.; Luan,H.; Jiang,Y.; Tian,X.; Zhang,S.
Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and reperfusion (I/R) injury in vivo, and elucidated its potential mechanism in vitro. Effects of cornin on cell viability, as well as expression of phospho-CREB and phospho-Akt in hypoxic H9c2 cells in vitro, and myocardial I/R injury in vivo, were investigated. Cornin attenuated hypoxia-induced cytotoxicity significantly in H9c2 cells in a concentration-dependent manner. Treatment of H9c2 cells with cornin (10 µM) blocked the reduction of expression of phospho-CREB...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cornin; Myocardial ischemia and reperfusion; CREB; Akt; Hypoxia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200608

Influence of the dopaminergic system, CREB, and transcription factor-κB on cocaine neurotoxicity
Provedor de dados: 56
Autores: Planeta,C.S.; Lepsch,L.B.; Alves,R.; Scavone,C.
Cocaine is a widely used drug and its abuse is associated with physical, psychiatric and social problems. Abnormalities in newborns have been demonstrated to be due to the toxic effects of cocaine during fetal development. The mechanism by which cocaine causes neurological damage is complex and involves interactions of the drug with several neurotransmitter systems, such as the increase of extracellular levels of dopamine and free radicals, and modulation of transcription factors. The aim of this review was to evaluate the importance of the dopaminergic system and the participation of inflammatory signaling in cocaine neurotoxicity. Our study showed that cocaine activates the transcription factors NF-κB and CREB, which regulate genes involved in cellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocaine; Apoptosis; NF-κB; CREB; BDNF; Neurotoxicity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100909

Icariin stimulates differentiation of bone marrow-derived mesenchymal stem cells (BM-MSCs) through activation of cAMP/PKA/CREB
Provedor de dados: 60
Autores: Lou,Dan; Ye,Jifeng; Yang,Lianhua; Wu,Zheng; Zheng,Wei; Zhang,Hui.
Icariin, a prenylated flavonol glycoside isolated from Epimedium, has been considered as a potential alternative therapy for osteoporosis. The present study aimed to clarify the detailed molecular mechanisms of action of icariin on osteoblast function, using bone marrow-derived mesenchymal stem cells (BM-MSCs). BM-MSCs were first stimulated by icariin. Then, gene and protein expression of cAMP/PKA/CREB signaling molecules were analyzed by RT-PCR and western blotting (WB), and alkaline phosphatase (ALP) was analyzed in cell lysates by ELISA. MTT assays indicated that icariin did not have significant effects on cell viability up to 1 µM. Icariin showed a dose-dependent effect on the alkaline phosphatase activity of BM-MSCs. WB analysis showed that icariin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Icariin/molecular mechanisms; BM-MSCs; CAMP; PKA; CREB.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100592