Consulta: subjectFacets:"Callus"
Registros recuperados: 27
Data/hora: 04/06/2024 16:41:08

Gene expression of a putative glutathione S-transferase is responsive to abiotic stress in embryogenic cell cultures of Cyclamen persicum
Provedor de dados: 69
Autores: Hoenemann,Claudia; Ambold,Juliane; Hohe,Annette.
Background: Cyclamen persicum is an economically important ornamental crop that is propagated exclusively through seeds as vegetative propagation using cuttings is not possible. Therefore a micropropagation method through somatic embryogenesis is of high interest; however the method suffers from low reliability concerning quality and quantity of the produced plantlets. A crucial step of the protocol is the removal of plant growth regulators (PGRs) that triggers embryo development. In order to get a better insight in this crucial step of the propagation process, a gene expression analysis has been set up using five different genes of glutathione S-transferases (GST) as these are known to be auxin responsive as well as stress reactive. Results: One out of...
Tipo: Journal article
Palavras-chave: Auxin; Callus; Micropropagation; Semisolid medium; Suspension culture.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100006

Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
Provedor de dados: 69
Autores: Cimino,Cecilia; Vairo Cavalli,Sandra; Spina,Francisco; Natalucci,Claudia; Priolo,Nora.
The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. (Asteraceae). Sections of cotyledons, previously disinfected by washing successively with ethanol 70&amp;ordm;, NaClO (10% w/v) and Tween 20 (0.05% v/v) and rinsing with sterile distilled water, were used as explants. For its initial culture, B5 medium supplemented with BA and 2,4-D solidified with phytagel was used, and a 63% survival was achieved. To obtain callus, two solid media were assayed (S1 and S2) using B5 medium supplemented with growth regulators (BA and 2,4-D or NAA and BA, respectively). The calli were grown at 25&amp;ordm;C during 45 days in darkness. Growth kinetics was studied using S1 medium...
Tipo: Journal article
Palavras-chave: Asteraceae; Callus; Rhizogenesis; Silybum marianum.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300012

Towards genetic transformation of local onion varieties
Provedor de dados: 69
Autores: Marinangeli,Pablo; Deluchi,Bernardo; Ríos,Raúl; Franzone,Pascual; Galmarini,Claudio; Rosselló,Fernando; Curvetto,Néstor.
The aim of this work was to explore the possibility of obtaining transgenic plants of onion varieties cultivated in Argentina, starting from calli induced from mature zygotic embryos, using two strains of Agrobacterium tumefaciens as transfection vectors. Mature embryos from three varieties of 'Valenciana' onion, Torrentina, Cobriza INTA and Grano de oro were in vitro cultivated for callus induction. After three to four months an average of 57.4% success for the three varieties was reached. Transformation was carried out with Agl1 or LBA 4404 A. tumefaciens strains, both carrying a binary vector containing the marker gene gus a and the selection gene nptII. Selection was done in callus induction medium containing 10 mgL-1 geneticin during three...
Tipo: Journal article
Palavras-chave: Agrobacterium tumefaciens; Allium cepa L.; Callus; Zygotic embryos.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300030

Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
Provedor de dados: 69
Autores: González,Germán Andrés; Pacheco,María Gabriela; Oneto,Cecilia Décima; Etchart,Valeria J; Kandus,Mariana V; Salerno,Juan Carlos; Eyherabide,Guillermo; Presello,Daniel; Lewi,Dalia Marcela.
Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC...
Tipo: Journal article
Palavras-chave: Callus; Inbred lines; Maize; Molecular markers; Regeneration; Somatic embryogenesis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100009

Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey
Provedor de dados: 69
Autores: Erisen,Semiha; Yorgancilar,Mustafa; Atalay,Emine; Babaoglu,Mehmet; Duran,Ahmet.
A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [?-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was...
Tipo: Journal article
Palavras-chave: Astragalus; Callus; Organogenesis; Plant regeneration.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600013

Melhoramento de plantas de interesse economico para a regiao amazonica atraves de tecnicas "in vitro".
Provedor de dados: 14
Autores: LEMOS, O. F. de; LAMEIRA, O. A.; MENEZES, I. C. de; MOTA, M. G. da C.; OKA, S.; SAITO, T.; SATO, M.; HIDAKA, T.; KOBAYASHI, S.
bitstream/item/67072/1/CPATU-Doc90P11.pdf
Tipo: Outras publicações técnicas (INFOTECA-E)
Palavras-chave: Brasil.; Calo; Melhoramento Genético Vegetal; Propagação Vegetativa.; Amazonia; Callus; Plant breeding; Vegetative propagation.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/395074

Propagacao: cultura de tecidos do mamoeiro.
Provedor de dados: 14
Autores: NUNES, R. F. de M.
Seminario apresentado sobre a cultura do mamoeiro. Propagacao vegetativa da cultura de tecidos do mamoeiro. Embriogenese somatica. Recuperacao de embriao interespecifico, cultivo do meristema, vantagens e desvantagens da micropropagacao.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Papaya; Meristem; Mamão; Carica Papaya; Cultura de Meristema; Cultura de Tecido; Micropropagação; Propagação Vegetativa; Callus; Tissue culture; Vegetative propagation.
Ano: 1995
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/132699

Returning to the roots: morphology, molecular phylogeny and classification of the Olivoidea (Gastropoda: Neogastropoda)
Provedor de dados: 5
Autores: Kantor, Yu. I.; Fedosov, A. E.; Puillandre, N.; Bonillo, C.; Bouchet, P.
The superfamily Olivoidea is broadly distributed in the world’s oceans mostly in coastal waters at tropical and subtropical latitudes. It encompasses around 30 Recent genera and 460 species. Two families – Olividae and Olivellidae – are classically recognized within the superfamily. Their shell is very characteristic due to the presence of a modified callused anterior end and a fasciole. Prior to the present work, neither the monophyly of the superfamily nor the relationships among its genera had been tested with molecular phylogenetics. Four genetic markers [cytochrome c oxidase subunit I (COI), 16S and 12S rRNA mitochondrial genes, and Histone 3 (H3) nuclear gene] were sequenced for 42 species in 14 genera. Additionally, 18 species were sequenced for COI...
Tipo: Text
Palavras-chave: Ancillariidae; Bellolividae; Benthobiidae; Callus; Digestive system; Mantle anatomy; Olividae; Pseudolividae; Radula.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00386/49713/50241.pdf

Alfalfa-Verticillium Albo-atrum Interactions : In Vitro Responses of Alfalfa Callus to Fungal Culture Filtrate and Cell Wall Components
Provedor de dados: 19
Autores: Koike, Masanori; Nanbu, Kouhei; Shimada, Tohru.
日本草地学会Journal of Japanese Society of Grassland Science 37(4):412-419(1992)より転載
Palavras-chave: Verticillium albo-atrum; Alfalfa; Callus; Cell wall components Culture filtrates; Disease resistance.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1772

Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration
Provedor de dados: 86
Autores: Houllou-Kido,Laureen Michelle; Kido,Ederson Akio; Falco,Maria Cristina; Silva Filho,Márcio de Castro; Figueira,Antônio Vargas de Oliveira; Nogueira,Neusa de Lima; Rossi,Mônica Lanzoni; Tulmann Neto,Augusto.
A plant regeneration method with cell suspension cultures of banana, and the effect of biobalistic on regeneration potential are described in this report. Somatic embryos of banana were obtained from indirect embryogenesis of male inflorescence of banana cultivar Maçã (AAB group). Part of the calluses formed (40%) showed embryogenic characteristics (nonfriable, compact and yellow color). The cell suspension, originated from embryogenic calluses, contained clusters of small tightly packed cells with dense cytoplasms, relatively large nuclei and very dense nucleoli. After four months of culture, somatic embryos started to regenerate. The maximum number of regenerated plants was observed between 45 and 60 days after embryo formation.In the first experiment,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musa; Cell suspension; Callus; Somatic differentiation; Biobalistic.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001100005

Standardized production of Phyllanthus tenellus Roxb. by plant tissue culture
Provedor de dados: 124
Autores: Victório,Cristiane Pimentel; Henriques,Anaize Borges; Tavares,Eliana Schwartz; Esquibel,Maria Apparecida; Lage,Celso Luiz Salgueiro.
Exigencies as ethic plant raw material are part of the needs of modern phytotherapy. Micropropagation offers opportunities to obtain mass propagation of superior genotypes in short time. This study aimed to develop a protocol of direct and indirect organogenesis of Phyllanthus tenellus Roxb. Nodal segments from plantlets obtained by in vitro germination were subcultured in modified Murashige and Skoog medium added with different plant growth regulators: IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), GA3 (3-giberelic acid) and KIN (kinetin). The highest proliferation rate was obtained using the combinations: IBA, KIN + GA3 (3.5 mg L-1) and IBA + KIN (2.4 mg L-1). Rooting was intensified after 40 days, reaching 100% for all media with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callus; Micropropagation; Medicinal plant.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902010000200015

Somatic embryogenesis in Citrus sinensis, C. reticulata AND C. nobilis x C. deliciosa
Provedor de dados: 63
Autores: Ricci,Adriana Patrícia; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Piedade,Sonia Maria de Stefano.
Most of the plant regeneration processes in citrus, through tissue culture, involve indirect somatic embryogenesis. The optimization of these processes is important for the development of in vitro plant improvement and micropropagation studies. Studies to evaluate the effect of different carbohydrates in somatic embryogenesis were conducted using calli from 'Ponkan' mandarin (Citrus reticulata, Blanco), 'Cravo' mandarin (C. reticulata), 'Itaboraí' sweet orange (C. sinensis L. Osbeck.), 'Valencia' sweet orange (C. sinensis) and 'Kinnow' mandarin (C. nobilis Loureiro x C. deliciosa Tenore). The culture medium used was MT supplemented with sucrose, galactose, glucose, maltose or lactose with the following concentrations of 18, 37, 75, 110, and 150 mM. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callus; Carbohydrate; Citrus; Somatic embryo.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100005

Enraizamento de estacas de lichia (Litchi chinensis Sonn.)
Provedor de dados: 63
Autores: Leonel,S.; Rodrigues,J. D.; Rodrigues,S. D.
Estudou-se os efeitos de auxilias exógenas e ácido bórico, no enraizamento de estacas de lichia (Litchi chinensis Sonn.). As estacas foram uniformizadas, com 25 cm de comprimento e 4 folhas cortadas pela metade. Cerca de 2,5 cm da base das mesmas foi mergulhado nos tratamentos: H2O; Boro 150 µg/ml; IBA 5.000 ppm, IBA 2.000 ppm; IBA 5.000 ppm + Boro 150 µg/ml; IBA 2.000 ppm + Boro 150 µg/ml; NAA 3.000 ppm; NAA 1.500 ppm; NAA 3.000 ppm + Boro 150 µg/ml; NAA 1.500 ppm + Boro 150 µ/g/ml. A estaquia foi realizada no mês de setembro (Hemisfério sul), sendo que as estacas foram colocadas em bandejas de isopor, tendo como substrato vermiculita e mantidas sob nebulização intermitente. Os resultados obtidos permitiram concluir que o IBA 5.000 ppm por 1 minuto foi o...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reguladores vegetais; Ácido bórico; Callus; Lichieira; Estacas; Enraizamento.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000200022

Micropropagation and callogenesis of Curcuma zedoaria Roscoe
Provedor de dados: 63
Autores: Miachir,Jeanette Inamine; Romani,Vera Lúcia Moretti; Amaral,Antônio Francisco de Campos; Mello,Marcia Ometto; Crocomo,Otto Jesu; Melo,Murilo.
Curcuma zedoaria Roscoe (zedoary) is a medicinal properties-bearing Zingiberaceae from which rhizomes are commercially exploited. The objective of this work was to establish an in vitro protocol for micropropagation and callogenesis of Curcuma zedoaria Roscoe as alternative to improve plant production, turning economically feasible the exploitation of its secondary metabolites which present medicinal properties. Micropropagation by using shoot apexes produced by rhizome and from in vitro plants were carried out on Murashige &amp;amp; Skoog medium supplemented with 2.0 mg L-1 benzyl amino purine and 30 g L-1 sucrose. Plantlets were satisfactorily acclimated to greenhouse conditions by using plastic cover for at least 10 days. Treatment with endomycorrhiza...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zingiberaceae; In vitro culture; Plant production; Cells suspension; Callus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400012

Production of Hevea brasiliensis transgenic embryogenic callus linesby Agrobacterium tumefaciens: roles of calcium
Provedor de dados: 106
Autores: Montoro, P.; Rattana, W.; Pujade-Renaud, V.; Michaux-Ferri_re, N.; Monkolsook, Y.; Kanthapura, R.; Adunsadthapong, S.
Palavras-chave: Production; Calcium; Callus.
Ano: 2003
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4979

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg
Provedor de dados: 3
Autores: Rossato, Marieli; Schumacher, Pedro Vitor; Costa Netto, Antônio Paulino da; Stein, Vanessa Cristina; Reis, Edésio Fialho dos; Vilela, Marlúcia Souza Pádua; Paiva, Luciano Vilela.
Campomanesia adamantium is a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatic embryogenesis; Growth regulator; Callus.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46358

Plant regeneration after long term callus culture in clones of Asparagus officinalis L.
Provedor de dados: 47
Autores: Pontaroli,A. C.; Camadro,E. L.
Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l-1 2,4-D and 1 mg.l-1 kinetin. Shoot primordia (SP) induction from &gt;18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone &amp;#8216;265' on MS medium with 5 mg.l-1 2iP, 1 mg.l-1 IAA, 3% sucrose and 0.9% agar. The highest...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Callus; Shoot primordia induction; Rooting; Medium x genotype interactions.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300009

Induction of secondary metabolite production by UV-C radiation in Vitis vinifera L. Öküzgözü callus cultures
Provedor de dados: 48
Autores: Cetin,Emine Sema.
BACKGROUND: The aim of the present work was to examine the role of UV-C irradiation on the production of secondary metabolites (total phenolic, total flavanols, total flavonols, catechin, ferulic acid and trans-resveratrol in phenolic compounds and &amp;#945;-, &amp;#946;-, &amp;#947;- &amp;#948;-tocopherols) in callus cultures. Studies on the effects of UV-C treatment on callus culture are seldom and generally focused on UV-B. However UV-C radiation play an important role in accumule secondary metabolites. RESULTS: In this study, callus cultures from Öküzgözü grape cultivar were initiated from leaf petiole explants. Calli formed after 6 weeks on the medium supplemented with 0.5 mg L-1 benzylaminopurine (BA), 0.5 mg L-1 indole acetic acid (IAA) on B5...
Tipo: Journal article
Palavras-chave: Callus; Secondary metabolite; UV-C treatment.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100037

In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe
Provedor de dados: 52
Autores: Delmail,David; Labrousse,Pascal; Lesouëf,Jean-Yves; Le Hir,François; Autret,Jean-Luc; Botineau,Michel.
Normania triphylla is an endemic species from Madeira island (Portugal) extinct in the wild since 1991. The aim of this work was to culture the meristems of this species in vitro and to multiply its shoots in order to preserve this endangered species. The best results in terms of multiplication were obtained in Murashige and Skoog medium supplemented with 10 µM 6‑benzylaminopurine (BAP). The number of shoots, the number of nodes and the number of leaves were the most important in this medium. However, the best results concerning the total shoot length were obtained when BAP was not supplemented into the medium and in the presence of 5 or 7.5 µM 1-naphtalene acetic acid (NAA). This is the first report on the in vitro culture of N. triphylla which could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Normania; Endemic species; Micropropagation; 6‑benzylaminopurine; Callus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400009

Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
Provedor de dados: 52
Autores: Mariano,André Bellin; Kovalhuk,Leonardo; Valente,Caroline; Maurer-Menestrina,Juliana; Pereira-Netto,Adaucto Bellarmino; Guerra,Miguel Pedro; Carnieri,Eva Gunilla Skare.
A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucaria angustifolia; Callus; Plant mitochondria; Respiratory chain.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006

In vitro Callus Induction and Plant Regeneration of Celosia argentea- An Important Medicinal Plant
Provedor de dados: 52
Autores: Bakar,Dalilah Abu; Ahmed,Bakrudeen Ali; Taha,Rosna Mat.
Celosia argentea (Var.) cristata (Amaranthaceae) is a widely cultivated ornamental plant, which has antibacterial, astringent, haemostatic, hypertensive, ophthalmic, and parasitic significance. This study describes a protocol for in vitro callus induction and plant regeneration from leaf and stem explants of C. argentea using Murashige and Skoog (MS) medium. Callus culture was initiated and established from seedling, leaf, and stem explants. Explants were cultured on MS medium supplemented with auxin alone (0.5 mg/L Naphthaleneacetic acid (NAA), 2, 4-Dicholorophenoxyacetic acid (2, 4-D). Green and red compact callus (98%) were induced using MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L Benzyladenine (BA). Two different concentrations (1.0 mg/L BA +...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celosia argentea; Amaranthaceae; In vitro; Micropropagation; Callus; Plant regeneration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600860

Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
Provedor de dados: 52
Autores: Hasbullah,Nor Azlina; Taha,Rosna Mat; Saleh,Azani; Mohamed,Normadiha.
In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gerbera jamesonii; Gamma irradiation; Callus; In vitro; Chlorophyll; Soluble protein.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300012

Detection of Specific Polypeptide(s) Synthesized during the Sequential Stages of Differentiation in Dioscorea species
Provedor de dados: 52
Autores: Kumar,Ashwani; Goyal,Suresh Chand; Dhansu,Pooja; Sheoran,Parvender; Kumar,Arvind; Verma,Ekta; Parshad,Jagdish; Singh,Anshuman.
ABSTRACTThe present investigation was aimed to detect the specific polypeptide(s) appeared during the sequential stages of differentiation. Among different explants, only nodal explants showed good results for callusing. Depending on the fresh and dry weight, best callus growth was observed on MS medium supplemented with NAA (2.5 mg/L) inDioscorea alata and 2, 4-D (2.0 mg/L) inD. deltoidea, respectively. This callus was used for the regeneration. Roots differentiation was observed on MS medium + NAA (2.0 mg/L) + IBA (0.5 mg/L) and shoots on MS medium + BAP (2.0 mg/L) + NAA (0.5 mg/L) in D. alata while in D. deltoidea, roots on RT medium + IAA (1.0 mg/L) and shoots on RT medium + BAP (1.0 mg/L) + NAA (0.5 mg/L). Continuous decrease was seen in the total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dioscorea alata; Dioscorea deltoidea; Callus; Differentiation; Polypeptides.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500667

Comparative Microbicidal Efficacy of Fractionated Extracts from In Vitro and In Vivo Raised Cells of Tinosporacordifolia Against MDR Pathogens
Provedor de dados: 52
Autores: Mittal,Jitendra; Singh,Abhijeet; Batra,Amla; Sharma,Madan Mohan.
The present study was conducted to explore the hidden potential of natural products synthesized in the medicinal plant Tinosporacordifolia. This plantis prioritized by National Medicinal Plant Board, New Delhi. Leaf and inter nodal segments were inoculated on MS Medium fortified with IBA (1.0 mg/L) produced callus after four weeks. The calli were brown due to phenolic substance secreted by the explant. This problem was overcome by using adjuvant PVP (0.1%). Further, secondary metabolites were isolated from callus and field leaf through soxhlet extractor and fractionated by using column chromatography. The antibacterial activity of these fractioned extracts from Tinosporacordifolia callus and leaf were seen against multi drug resistance bacteria viz.,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Callus; Natural products; PVP; Antimicrobial activity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100406

The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract
Provedor de dados: 58
Autores: Bellato,Cláudia de M.; Garcia,Andréia K.M.; Mestrinelli,Fabiana; Tsai,Siu M.; Machado,Marcos A.; Meinhardt,Lyndel W.
An in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2DE; Callus; Pathogenicity; Protein; Xylella-citrus interaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)
Provedor de dados: 117
Autores: Avilés,Fabiola; Ríos,Darcy; González,Ricardo; Sánchez-Olate,Manuel.
To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 cm², previously sterilized, were placed in each culture medium, supplemented with 21.4 µM naphtalene acetic acid (NAA) and 8.8 µM 6-benzylaminopurine (BAP). A completely random design was used; the experimental unit was a Petri dish with six callus explants. Each treatment was composed of seven Petri dishes. Callogenesis (%), nodular calli (%) and histology of calli explants were evaluated at 4 weeks by staining 10 µm slices with safranine-fastgreen. There were...
Tipo: Journal article
Palavras-chave: Auxins; Citoquinines; Walnut; Callus; Leaves.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300020

In vitro morphogenic response from zygotic embryos of Genipa americana
Provedor de dados: 65
Autores: Oliveira,Annie Carolina Araújo de; Machado,Caroline de Araújo; Oliveira,Leila Albuquerque Resende de; Silva,Ana Veruska Cruz da; Lédo,Ana da Silva.
ABSTRACT: The aim of this study was to evaluate the in vitro morphogenic potential of genipap (Genipa americana L.) zygotic embryos. Seeds obtained from ripe fruits had their zygotic embryos excised and inoculated in MS medium with 4.44µM of 6-benzylaminopurine (BAP) and supplemented with 0.0; 1.07; 2.14 and 3.21µM of naphthalene acetic acid (NAA). The potential of explants regeneration and the shoot length and number of leaves in plantlets were evaluated. The in vitro regeneration of genipap is possible from the conversion of zygotic embryos in a MS medium with 4.44µM BAP supplemented with 3.21µM NAA.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant tissue culture; Plant growth regulators; Callus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000402