Consulta: subjectFacets:"Candida dubliniensis"
Registros recuperados: 2
Data/hora: 10/06/2024 17:08:21

Candida dubliniensis in a Brazilian family with an HIV 1- infected child: identification, antifungal susceptibility, drug accumulation and sterol composition
Provedor de dados: 58
Autores: Melo,Nadja Rodrigues de; Taguchi,Hideaki; Culhari,Vitoria V.P.; Sano,Ayako; Fukushima,Kazutaka; Miyaji,Makoto; Manning,Nigel; Kelly,Steven L.; Vilela,Marluce S.
This study investigated the prevalence of C. dubliniensis in a Brazilian family with an HIV - infected child. A total of 42 oral isolates were obtained from eight family members. The identification of C. dubliniensis was performed by polymerase chain reactions (PCR) using primers against a specific sequence of the C. dubliniensis cytochrome b gene. Only the HIV-infected child and his grandmother were colonized by C. dubliniensis. In this study C. dubliniensis isolated from the HIV-infected child exhibited high susceptibility for azoles tested with MICs of 0.125 and 0.5 µg/mL for voriconazole and fluconazole, respectively. Accumulation of [³H] fluconazole in C. dubliniensis isolated from the HIV-infected child was slightly reduced in comparison to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida dubliniensis; Antifungal agents; Sterol composition.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300008

A simple and reliable PCR-restriction fragment length polymorphism assay to identify Candida albicans and its closely related Candida dubliniensis
Provedor de dados: 58
Autores: Ge,Yi Ping; Wang,Le; Lu,Gui Xia; Shen,Yong Nian; Liu,Wei Da.
Candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. Due to its close similarity to C. albcians, conventional methods based on phenotypic traits are not always reliable in identification of C. dubliniensis. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay to identify and discriminate between the two closely related species. The D1/D2 region of 28S rDNA was amplified by PCR and enzymatically digested by ApaI and BsiEI respectively. PCR products of both species were digested into two fragments by ApaI, but those of other yeast species were undigested. BsiEI cut the PCR products of C. albicans into two fragments but not those of C. dubliniensis. Thus two species were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida albicans; Candida dubliniensis; PCR-restriction fragment length polymorphism; Identification; Differentiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300004