Consulta: subjectFacets:"Cell characterization"
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Can widely used cell type markers predict the suitability of immortalized or primary mammary epithelial cell models?
Provedor de dados: 48
Autores: Ontsouka,Edgar Corneille; Bertschi,Janique Sabina; Huang,Xiao; Lüthi,Michael; Müller,Stefan; Albrecht,Christiane.
BACKGROUND: Mammary cell cultures are convenient tools for in vitro studies of mammary gland biology. However, the heterogeneity of mammary cell types, e.g., glandular milk secretory epithelial or myoepithelial cells, often complicates the interpretation of cell-based data. The present study was undertaken to determine the relevance of bovine primary mammary epithelial cells isolated from American Holstein (bMEC US) or Swiss Holstein-Friesian (bMEC CH) cows, and of primary bovine mammary alveolar epithelial cells stably transfected with simian virus-40 (SV-40) large T-antigen (MAC-T) for in vitro analyses. This was evaluated by testing their expression pattern of cytokeratin (CK) 7, 18, 19, vimentin, and &amp;#945;-smooth muscle actin (&amp;#945;-SMA....
Tipo: Journal article
Palavras-chave: Cell characterization; Flow cytometry; Gene expression; Lactation marker; Mammalian; Mammary epithelial cell.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100001