Consulta: subjectFacets:"Cell division"
Registros recuperados: 17
Data/hora: 10/06/2024 07:42:42

Differential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems
Provedor de dados: 74
Autores: Martins,Polyana K.; Jordão,Berenice Q.; Yamanaka,Naoki; Farias,José R.B.; Beneventi,Magda A.; Binneck,Eliseu; Fuganti,Renata; Stolf,Renata; Nepomuceno,Alexandre L.
Drought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water-stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell division; Differential display; Gene expression; Real time PCR; Soybean; Water stress.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300019

Genes involved in cell division in mycoplasmas
Provedor de dados: 74
Autores: Alarcón,Frank; Vasconcelos,Ana Tereza Ribeiro de; Yim,Lucia; Zaha,Arnaldo.
Bacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a multiprotein complex called the septal ring. Mycoplasmas are cell wall-less bacteria presenting a reduced genome. Thus, it was important to compare their genomes to analyze putative genes involved in cell division processes. The division and cell wall (dcw) cluster, which in E. coli and B. subtilis is composed of 16 and 17 genes, respectively, is represented by only three to four genes in mycoplasmas. Even the most conserved protein, FtsZ, is not present in all mycoplasma genomes analyzed so far. A model for the FtsZ protein from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell division; Mycoplasma spp; Genomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200003

Influence de l'azote et du phosphore sur la croissance et la toxicité de Prorocentrum lima (Ehrenberg) Dodge
Provedor de dados: 5
Autores: Morlaix, Martine; Lassus, Patrick.
Very few attempts have been made in order to investigate the role of nutrients on cellular growth and toxin production in dinoflagellates associated with DSP (Diarrhetic Shellfish Poison). Some preliminary results are presented here, aiming at a better knowledge of inorganic nitrogen and organic phosphorus effects upon division rate and toxin content of Prorocentrum lima (PL2V spanish strain). Nitrogen have a positive effect on growth rate, up to a threshold value of 880muM.l-1. Above this threshold the rate decreases. There is no effect on toxin content since okadaic acid and DTX1 cell contents remain low (4.8 +/- 0.5 pg.cell.-1) and steady. Phosphorus effect upon division rate follows a non-linear relationship, whereas cellular toxic content is inversely...
Tipo: Text
Palavras-chave: Croissance; Toxicité; Concentration chimique; Division cellulaire; Production; Espagne; Phosphore; Azote; Europe; Nutriment; Milieu marin; Zone côtière; Ecotoxicologie; Pyrrophycophyta; Algae; Thallophyta; Okadaïque acide; Prorocentrum lima; Growth; Toxicity; Chemical concentration; Cell division; Production; Spain; Phosphorus; Nitrogen; Europe; Nutrient; Marine environment; Coastal zone; Ecotoxicology; Pyrrophycophyta; Algae; Thallophyta.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00133/24469/22483.pdf

Phytochrome-like responses in Euglena: a low fluence response that reorganizes the spectral dependence of the high irradiance response in long-day photoperiodic induction of cell division
Provedor de dados: 19
Autores: Bolige, Aoen; Goto, Ken.
Irradiance spectra change spatiotemporally, and angiosperms adapt accordingly, mainly through phytochromes. This study challenges the long-held belief that the flagellated alga Euglena gracilis lacks phytochromes and is therefore unaffected by spectral changes. We photoautotrophically cultured the alga under continuous light (LL), then transferred it to darkness. After about 26 h in darkness, different irradiations for 3 h enabled cell division in dark-arrested G2 cells evoking a high-irradiance response (HIR). The spectral characteristics of the irradiation during the LL period (pre-irradiation) defined the spectral sensitivity in the subsequent dark period. LL with light rich in the red spectrum led to a HIR to the red spectrum (R-HIR), whereas light...
Palavras-chave: Cell division; Circadian rhythms; End-of-day; Euglena; Photoperiodism; Phytochrome; Shade avoidance.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/720

Cytotoxic and Genotoxic Effects of Macroptilium lathyroides
Provedor de dados: 87
Autores: SILVA,M.S.A.; YAMASHITA,O.M.; ROSSI,A.A.B.; CONCENÇO,G.; CARVALHO,M.A.C.; FELITO,R.A.
ABSTRACT: The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 oC) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abnormalities; Bioassays; Cell division; Phasey bean; Mitotic index.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100244

Molecular evolution in bacteria: cell division
Provedor de dados: 59
Autores: Trevors,J.T.
Molecular evolution in bacteria is examined with an emphasis on the self-assembly of cells capable of primitive division and growth during early molecular evolution. Also, the possibility that some type of encapsulation structure preceeded biochemical pathways and the assembly of genetic material is examined. These aspects will be considered from an evolutionary perspective.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell division; Molecular evolution; Bacteria; Self-assembly.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400001

Determinação das castas em Scaptotrigona postica (Latreille) (Hymenoptera, Apidae, Meliponini): diferenciação do ovário
Provedor de dados: 104
Autores: Santos,Thaís da Cruz Alves dos; Cruz-Landim,Carminda da.
Both castes of Scaptotrigolia postica (Latreille, 1804) possess four ovarioles in each ovary. Queen and workers have the same ovarian development during the larvallife, but in lhe late larval stage the queen ovary beco me larger. During pupation a higher rate of cell division is observed in queen ovarioles and a higher rale of cell death in workers. Newly emerged workers have short ovarioles with differenriatcd germarium and vitellarium while queens have very long ovarioles with only germarium. Caste deterrnination in rhis species of bee is trophic, but lhe food does not differ in quality, only in quantiry. The food differences only beco me effecri ve by the end of larval stage when the queen larvae have lhe opportunity of eat more. In this way lhe ovary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stingless beco castes; Ovary differentiation; Cell division; Cell dearh.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752002000300007

In vitro callogenesis induction of Guarianthe skinneri (Bateman) Dressler &amp;amp; W.E. Higgins (Orchidaceae)
Provedor de dados: 109
Autores: Coutiño-Cortés,Ana Gabriela; Bertolini,Vincenzo; Iracheta-Donjuan,Leobardo; Ruíz-Montoya,Lorena; Valle-Mora,Javier Francisco.
Abstract: Guarianthe skinneri (Bateman) Dressler &amp; W.E. Higgins, is a native orchid from Mexico, considered as threatened species in the NOM-ECOL-059-SEMARNAT-2010, mainly due to the disappearance of its natural habitat and illegal collection during the flowering season. The aim of this research was to induce in vitro callogenesis using different types of explants and phytoregulators, in order to establish a mass production technique as a contribution to the conservation of this species. We evaluated leaf and pseudobulb pith explants growing in semi-solid medium MS, adding BAP, 2, 4-D, Kin, the combination of BAP/2 and 4-D/Kin/Sad and a control without any type of plant growth regulators. Statistical analysis showed that pseudobulb pith explants are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity conservation; Cell division; Oxidation; Phytoregulator.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000200254

Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L.
Provedor de dados: 3
Autores: Santana, Gleuvânia Marques; Sousa, Josefa Janaína do Anjos; Peron, Ana Paula.
This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p &lt; 0.05). From the results, we observed that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tecnolologia de alimentos  citogenética azo dye; Cell division; Cellular aberrations; Cytotoxicity.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119

Toxicity at the cellular level of artificial synthetic flavorings
Provedor de dados: 3
Autores: Sales, Ila Monize Sousa; Oliveira, Jussara Damascena de; Santos, Fabelina Karollyne Silva dos; Feitoza, Lidiane de Lima; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
The goal of the present study was to evaluate the cytotoxicity and genotoxicity of artificial synthetic flavoring agents Cookie and Tutti-frutti. To this end, root meristem cells of Allium cepa L. were exposed to these substances in exposure times of 24 and 48 hours using individual doses of 0.3; 0.6 and 0.9 mL and doses combined as follows: 0.3 mL + 0.3 mL; 0.6 mL and 0.9 mL + 0.6 mL + 0.9 mL. After applying the treatments, root meristems were fixed, hydrolyzed, stained and analyzed a total of 5,000 cells using an optical microscope to evaluate each dose and combined treatment. All three doses of Cookie flavoring and combined treatments significantly inhibited cell division of the tissue studied. Doses of Tutti-Frutti caused no change in cell division...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30751

Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children
Provedor de dados: 3
Autores: Silva, Geysa Janne Sousa; Santos, Fabelina Karollyne Silva dos; Marques, Márcia Maria Mendes; Peron, Ana Paula.
This study aimed to evaluate the cytotoxicity and genotoxicity and determine the LC50 concentration of powdered infant formulas widely marketed in South American countries. To this, milk samples, called as A, B, C and D, were analyzed in root meristem cells of Allium cepa, at concentrations of 0.075; 0.15 and 0.30 g mL-1, for 24 and 48 hours; and through cell viability in culture of normal line cells, via MTT test, for 24 hours, in the concentrations 0.018; 0.0375; 0.075 and 0.15 g mL-1. In A. cepa, all dairy products in the three concentrations caused significant inhibition of cell division in the meristems within the first 24 hours of exposure. In the in vitro evaluation, all milk formulas at 0.15 g mL-1, as well as milk A at a concentration of 0.037 g...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infant formula; Cell division; Meristematic tissue; Cell viability; LC50.
Ano: 2020
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46856

Cell Division in genus Corynebacterium: protein-protein interaction and molecular docking of SepF and FtsZ in the understanding of cytokinesis in pathogenic species
Provedor de dados: 42
Autores: OLIVEIRA JR,ALBERTO F.; FOLADOR,EDSON L.; GOMIDE,ANNE C.P.; GOES-NETO,ARISTÓTELES; AZEVEDO,VASCO A.C.; WATTAM,ALICE R.
ABSTRACT The genus Corynebacterium includes species of great importance in medical, veterinary and biotechnological fields. The genus-specific families (PLfams) from PATRIC have been used to observe conserved proteins associated to all species. Our results showed a large number of conserved proteins that are associated with the cellular division process. Was not observe in our results other proteins like FtsA and ZapA that interact with FtsZ. Our findings point that SepF overlaps the function of this proteins explored by molecular docking, protein-protein interaction and sequence analysis. Transcriptomic analysis showed that these two (Sepf and FtsZ) proteins can be expressed in different conditions together. The work presents novelties on molecules...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell division; Corynebacterium; FtsZ; SepF; Molecular docking.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502179

Action of Aqueous Extracts of Phyllanthus niruri L. (Euphorbiaceae) leaves on Meristematic Root Cells of Allium cepa L.
Provedor de dados: 42
Autores: NEVES,ERASMOVLANE S.B.; FERREIRA,PAULO MICHEL PINHEIRO; LIMA,LEONARDO H.G.M.; PERON,ANA PAULA.
This study aimed to evaluate the effects of aqueous extracts of dried Phyllanthus niruri L. (stonebreaker) leaves on Allium cepa L. root meristem cells at four concentrations, 0.02 (usual concentration), 0.04, 0.06 and 0.08mg/mL and exposure times of 24 and 48 hours. For each concentration we used a group of five onion bulbs that were first embedded in distilled water and then transferred to their respective concentrations. The radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were prepared by the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5000 for each control and exposure time. The calculated mitotic indices were subjected to the Chi-squared statistical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plant; Stonebreaker; Cell division; Cellular aberrations; Allium cepa.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301131

Differential regulation of vitamin D receptor expression in distinct leukemic cell lines upon phorbol ester-induced growth arrest
Provedor de dados: 56
Autores: Folgueira,M.A.A.K.; Federico,M.H.H.; Roela,R.A.; Maistro,S.; Katayama,M.L.H.; Brentani,M.M.
A close correlation between vitamin D receptor (VDR) abundance and cell proliferation rate has been shown in NIH-3T3 fibroblasts, MCF-7 breast cancer and in HL-60 myeloblastic cells. We have now determined if this association occurs in other leukemic cell lines, U937 and K562, and if VDR content is related to c-myc expression, which is also linked to cell growth state. Upon phorbol myristate acetate (PMA) treatment, cells from the three lineages (HL-60, U937 and K562) differentiated and expressed specific surface antigens. All cell lines analyzed were growth inhibited by PMA and the doubling time was increased, mainly due to an increased fraction of cells in the G0/G1 phase, as determined by flow cytometry measurements of incorporated bromodeoxyuridine and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Receptors; Calcitriol; Cell division; Cell differentiation; Leukemia; Phorbol esters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500011

Excavation of attractor modules for nasopharyngeal carcinoma via integrating systemic module inference with attract method
Provedor de dados: 56
Autores: Jiang,T.; Jiang,C.-Y.; Shu,J.-H.; Xu,Y.-J.
The molecular mechanism of nasopharyngeal carcinoma (NPC) is poorly understood and effective therapeutic approaches are needed. This research aimed to excavate the attractor modules involved in the progression of NPC and provide further understanding of the underlying mechanism of NPC. Based on the gene expression data of NPC, two specific protein-protein interaction networks for NPC and control conditions were re-weighted using Pearson correlation coefficient. Then, a systematic tracking of candidate modules was conducted on the re-weighted networks via cliques algorithm, and a total of 19 and 38 modules were separately identified from NPC and control networks, respectively. Among them, 8 pairs of modules with similar gene composition were selected, and 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nasopharyngeal carcinoma; Protein-protein interaction; Attract; Module; Cell division.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800602

Heparan sulfate and cell division
Provedor de dados: 56
Autores: Porcionatto,M.A.; Nader,H.B.; Dietrich,C.P.
Heparan sulfate is a component of vertebrate and invertebrate tissues which appears during the cytodifferentiation stage of embryonic development. Its structure varies according to the tissue and species of origin and is modified during neoplastic transformation. Several lines of experimental evidence suggest that heparan sulfate plays a role in cellular recognition, cellular adhesion and growth control. Heparan sulfate can participate in the process of cell division in two distinct ways, either as a positive or negative modulator of cellular proliferation, or as a response to a mitogenic stimulus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparan sulfate; Glycosaminoglycan; Proteoglycan; Cell division; Cell cycle; Growth factors.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500006

Changes in growth variables and potassium content in leaves of Black Barley in response to NaCl
Provedor de dados: 61
Autores: Abu-Al-Basal,Mariam A.; Yasseen,Bassam T.
Much attention is being focused on the Black barley (Hordeum distichum L.) as a local cultivar offering good model for a cereal crop has traits of resistance to drought and salinity during vegetative growth stages. Although Black was sensitive to salt stress during germination, it developed gradual tolerance with age and proved very tolerant during growth and development stages. The data of study clearly revealed how this cultivar was superior over Arivat (Hordeum vulgare L.) in many physiological aspects such as leaf growth variables (i.e. rate and duration) and processes (i.e. cell division), tiller production and potassium content. Black barley had more tillers, faster rate and longer duration of growth processes which was accompanied with potassium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Black barley; Hordeum distichum L.; Cell division; Growth rate; Growth duration; Leaf growth; Arivat; Hordeum vulgare L.; Potassium; Salt stress.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000400002