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Data/hora: 10/06/2024 12:37:17

Mechanism of hif-1α mediated hypoxia-induced permeability changes in bladder endothelial cells
Provedor de dados: 56
Autores: Liu,C.; Shui,C.L.; Wang,Q.; Luo,H.; Gu,C.G.
This study aimed to investigate the mechanism of hypoxia-inducible factor-1 alpha (HIF-1α) mediated hypoxia-induced permeability changes in bladder endothelial cells. Models of in vitro hypoxic cell culture of bladder cancer, bladder cancer cells with low HIF-1α expression and HIF-1α RNA interference (RNAi) expression vector were established. Western blot and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of HIF-1α and vascular endothelial growth factor (VEGF) in each group. Bladder cell permeability was determined. Results showed that protein and mRNA expression of HIF-1α and VEGF at 3 and 12 h of hypoxia were significantly higher than normal control (P&lt;0.05), and peaked at 12 h. HIF-1α and VEGF expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bladder cancer; Hypoxia inducible factor-1α; VEGF; Cell permeability; YC-1.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200616