Consulta: subjectFacets:"Cell viability"
Registros recuperados: 16
Data/hora: 08/06/2024 17:10:05

Producción de taxoides en cultivos in vitro de callos y células elicitadas de Taxus globosa.
Provedor de dados: 32
Autores: Barrales Cureño, Hebert Jair.
Los objetivos de este trabajo fueron: establecer un método de propagación in vitro para callos y células en suspensión de Taxus globosa, se realizaron pruebas de viabilidad celular; se elicitaron suspensiones celulares con jasmonato de metilo, etanol y una combinación de peróxido de hidrógeno con butionina de sulfoximina. Se cuantificaron tres taxoides: taxol, cefalomanina y 10-diacetil baccatina por Cromatografía Liquida de Alta Resolución en cultivos in vitro de callos, células elicitadas y hojas de T. globosa. La inducción de los callos se originó a los 40 días, el mayor porcentaje se obtuvo con el tratamiento de picloram y polivinil pirrolidona. El análisis por Cromatografía Líquida de hojas de un extracto crudo en metanol registró la mayor acumulación...
Palavras-chave: 10-diacetil baccatina; Cefalomanina; Taxol; Anticáncer; Fitohormonas; Viabilidad celular; 10-diacetyl baccatin; Cephalomannine; Phytohormones; Cell viability; Botánica; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/2036

Viability in protoplasts and cell suspensions of Coffea arabica cv. Catimor
Provedor de dados: 69
Autores: Fernandez-Da Silva,Rafael; Menéndez-Yuffá,Andrea.
Two methods were evaluated in order to assess the viability of protoplasts and cell suspensions of Coffea arabica cv. Catimor used in a protocol of transformation by electroporation. One method consisted of staining with 1 % Evans blue and the other staining with 1% 3-[4,5-dimethyltiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT). When Evans blue was applied to viable cells and protoplasts they either did not stain or acquired a faint blue colour. However, it was difficult to distinguish the non-viable cells in the wide spectrum of clear blue tonalities. In contrast, with the MTT assay only the viable cells and protoplasts reduced this salt to the red coloured formazan; viable and non-viable cells were distinguished more clearly with MTT than with Evans...
Tipo: Journal article
Palavras-chave: Cell suspensions; Cell viability; Coffee; Evans blue; MTT; Protoplasts.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500016

Effects of temperature and salinity on the survival of Bonamia ostreae, a parasite infecting flat oysters Ostrea edulis
Provedor de dados: 5
Autores: Arzul, Isabelle; Gagnaire, Beatrice; Bond, Celine; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster Ostrea edulis. The parasite has been described in various ecosystems from estuaries to open sea, but no clear correlation has yet been demonstrated between disease development and environmental parameters. In this study, the effect of temperature and salinity on the survival of purified parasites maintained in vitro in seawater was investigated by flow cytometry. Purified parasites were incubated in various seawater media (artificial seawater, natural seawater, seabed borewater) at various temperatures (4, 15 and 25°C) and subjected to a range of salinities from 5 to 45 g l–1. Parasites were collected after 12, 24 and 48 h of...
Tipo: Text
Palavras-chave: Salinity tolerance; Temperature tolerance; Cell viability; In vitro assays; Flow cytometry; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6443.pdf

Integrin-linked kinase is involved in lactoferrin-induced anchorage-independent cell growth and survival in PC12 cells
Provedor de dados: 19
Autores: Ishii, Toshiaki; Uto, Tomoka; Mori, Kaori; Fujikawa, Ryu; 石井, 利明.
Aims: Bovine lactoferrin (bLf) causes anchorage-independent cell growth in PC12 cells. The present study investigated the mechanisms involved in bLf-induced anchorage-independent cell growth and survival in PC12 cells. Main methods: The number of adherent cells and suspended cells was estimated separately by using a methyl thiazol tetrazolium (MTT) assay, and the sum of both optical density (O.D.) (570 nm) values was used as a measure of the total number of cells. Key findings: Integrin-linked kinase (ILK) plays an important role in integrin and growth factor signaling pathways. Stable transfection of PC12 cells with a dominant negative kinase-deficient mutant of ILK (DN-ILK) inhibited bLf-induced anchorage-independent cell growth. The ILK activity in the...
Palavras-chave: Bovine lactoferrin; PC12 cell; Integrin-linked kinase; Integrin; P38 MAPK; Cell suspension; Cell adhesion; Cell growth; Cell viability; Dominant-negative mutant; Anchorage-independent growth.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2675

Effects of spermicidal extracts of Ananas comosus (pineapple) and Sapindus saponaria (jaboncillo) on cell viability, cytotoxicity and apoptosis
Provedor de dados: 98
Autores: Ospina Medina,Luisa; Álvarez Gómez,Ángela; Cortés Mancera,Fabian; Cadavid Jaramillo,Ángela; Cardona Maya,Walter.
Introduction: there are natural products from different fruits and plants that are effective as spermicides, but it is important that they should have little or no cytotoxic effect on epithelial cells. Currently available spermicides with nonoxynol-9 cause vaginal irritation and damage to the vaginal mucosa, the uterine epithelium, and the microbial flora of the vagina. Objective: to elucidate the effect on cell viability, cytotoxicity and apoptosis of spermicidal extracts of Ananas comosus (L.) Merr. and Sapindus saponaria L. over HeLa cell line. Methods: both extracts were evaluated on HeLa cell line using the novel ApoTox-Glo&amp;#8482; Triplex Assay to determine whether cell viability, cytotoxicity and apoptosis were affected. Results: it was...
Tipo: Journal article
Palavras-chave: Natural compounds; Spermicides; Cell cytotoxicity; Cell viability; Cell apoptosis; Ananas comosus; Sapindus saponaria.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962013000400005

Addition of proteic nitrogen during alcoholic fermentation for the production of cachaça
Provedor de dados: 63
Autores: Jeronimo,Elisangela Marques; Oliveira,Evelyn de Souza; Souza,Elson Luíz Rocha; Silva,Marcelo de Almeida; Serra,Gil Eduardo.
Cachaça is the denomination of a typical and exclusive Brazilian spirit produced from the distillation of fermented sugarcane juice must. The objective of this study was to evaluate the effect of adding yeast extract to the sugarcane juice used for sugarcane liquor production, because for the artisanal process no studies are available on nitrogen addition nor beverage quality, involving nitrogen complementation. Results of previous studies in the laboratory scale showed that sugarcane juice complementation with proteic nitrogen can be a beneficial practice for yeast multiplication and cellular growth, and also for the improvement of fermentation yield and liquor productivity. In this pilot scale study, using recycled yeast, the addition of proteic nitrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast extract; Cell viability; Volatile compounds; Sensory analysis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200009

Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children
Provedor de dados: 3
Autores: Silva, Geysa Janne Sousa; Santos, Fabelina Karollyne Silva dos; Marques, Márcia Maria Mendes; Peron, Ana Paula.
This study aimed to evaluate the cytotoxicity and genotoxicity and determine the LC50 concentration of powdered infant formulas widely marketed in South American countries. To this, milk samples, called as A, B, C and D, were analyzed in root meristem cells of Allium cepa, at concentrations of 0.075; 0.15 and 0.30 g mL-1, for 24 and 48 hours; and through cell viability in culture of normal line cells, via MTT test, for 24 hours, in the concentrations 0.018; 0.0375; 0.075 and 0.15 g mL-1. In A. cepa, all dairy products in the three concentrations caused significant inhibition of cell division in the meristems within the first 24 hours of exposure. In the in vitro evaluation, all milk formulas at 0.15 g mL-1, as well as milk A at a concentration of 0.037 g...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infant formula; Cell division; Meristematic tissue; Cell viability; LC50.
Ano: 2020
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46856

Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
Provedor de dados: 48
Autores: Yin,Lei-Miao; Wei,Ying; Wang,Wen-Qian; Wang,Yu; Xu,Yu-Dong; Yang,Yong-Qing.
BACKGROUND: BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In this study, we examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection using rat airway smooth muscle cells. RESULTS: The WST-1 measurements obtained from the combined detection were consistent with those obtained from the separate detection, which suggested that the addition of BrdU 2 h prior to the WST-1 analysis did not affect the WST-1 results. The BrdU measurements obtained from the...
Tipo: Journal article
Palavras-chave: BrdU; WST-1; Cell proliferation; Cell viability; Airway smooth muscle cells.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100069

Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation
Provedor de dados: 52
Autores: Uberti,Marcela Fornari; Vieira,Felipe do Nascimento; Salência,Helena Ragibo; Vieira,Genyess da Silva; Vinatea,Luis Alejandro.
Aiming at assessing the cryopreservation potential of Litopenaeus vannamei sperm cells, 74 spermatophores were manually extracted from the sexually mature individuals. After the toxicity test to define the cryoprotectant concentration, suspensions of spermatic cells were cryopreserved in the groups in freezing solutions comprising different cryoprotectants such as dimethyl sulfoxide (DMSO) and ethylene glycol (EG) at 10% concentration. Each treatment was divided in subgroups for storage in liquid nitrogen during 0, 30, 60 and 90 days, in triplicate. After thawing at 25ºC for 40 seconds, cell viability in the suspensions was analyzed under the microscope in eosin-nigrosin stain and flow cytometry. There were no significant differences between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryogenics; Semen; Marine shrimp; Cell viability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300010

Cell density and solvent are critical parameters affecting formazan evaluation in MTT assay
Provedor de dados: 52
Autores: Gasque,Kellen Cristina da Silva; Al-Ahj,Luana Polioni; Oliveira,Rodrigo Cardoso; Magalhães,Ana Carolina.
The aim of this study was to establish the more accurate protocol for fibroblast cell viability using MTT assay. NIH/3T3 fibroblasts were seeded at the following cell densities: 3.125x10³; 1.156x10(4); 3.125x10(4); 1.156x10(5) and 3.125x10(5) cells/cm². Following 24h of seeding, MTT was added to the wells. After 4h of the MTT addition, different solvents were added to solubilize the formazan crystals: 1) HCl/SDS group- 20% SDS and 0.01 M HCl; 2) EtOH/ HAc group-50% ethanol and 1% acetic acid; 3) DMSO group- 99.5% dimethyl sulfoxide; and 4) PropOH group- 99.5% isopropanol. The absorbance values were measured using a spectrophotometer at 570 nm. The data were analyzed by 2-way ANOVA (p&lt;0.05) and showed that the absorbance average varied according to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell viability; MTT assay; Fibroblast NIH/3T3; Solvents.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300011

Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
Provedor de dados: 52
Autores: Vasconcelos,André Belico de; Souza,Patrícia Castanheira de; Varago,Fabiana Cristina; Lagares,Monique de Albuquerque; Santoro,Marcelo Matos.
The heat conduction microcalorimeter can be used to evaluate the metabolic rates of the sperm cell. Two ejaculates of four stallions were cooled to +5ºC and checked for sperm motility (bright field microscopy), viability (eosin 3%), functional membrane integrity (hyposmotic swelling test), and heat production (microcalorimetry). Glucose and sperm cell concentrations were determined in order to measure the heat outputs resulting from sperm metabolism. Sperm viability, membrane integrity and sperm motility did not differ among the different glucose concentrations tested. Nevertheless, the highest heat output detected by the microcalorimeter was obtained with 6 mM glucose and 10(8) spermatozoa/mL. Since conduction microcalorimetry offered additional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcalorimetry; Glucose; Spermatozoa; Sperm metabolism; Cell viability; Equine.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010

Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury
Provedor de dados: 56
Autores: Zhang,Weiwei; Li,Ying; Wang,Peng.
Long non-coding RNAs (lncRNAs) play an important role in the pathogenesis of cardiovascular diseases, especially in myocardial infarction and ischemia/reperfusion (I/R). However, the underlying molecular mechanism remains unclear. In this study, we determined the role and the possible underlying molecular mechanism of lncRNA-ROR in myocardial I/R injury. H9c2 cells and human cardiomyocytes (HCM) were subjected to either hypoxia/reoxygenation (H/R), I/R or normal conditions (normoxia). The expression levels of lncRNA-ROR were detected in serum of myocardial I/R injury patients, H9c2 cells, and HCM by qRT-PCR. Then, levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were measured by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA; Ischemia/reperfusion (I/R); Hypoxia/reoxygenation (H/R); Cell viability; Apoptosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600609

Propofol inhibits lung cancer cell viability and induces cell apoptosis by upregulating microRNA-486 expression
Provedor de dados: 56
Autores: Yang,N.; Liang,Y.; Yang,P.; Yang,T.; Jiang,L.
Propofol is a frequently used intravenous anesthetic agent. Recent studies show that propofol exerts a number of non-anesthetic effects. The present study aimed to investigate the effects of propofol on lung cancer cell lines H1299 and H1792 and functional role of microRNA (miR)-486 in these effects. H1299 and/or H1792 cells were treated with or without propofol and transfected or not with miR-486 inhibitor, and then cell viability and apoptosis were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry. The expression of miR-486 was determined by quantitative real-time polymerase chain reaction (qRT-PCR) with or without propofol treatment. Western blot was performed to analyze the protein expression of Forkhead...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; Lung cancer; Cell viability; Cell apoptosis; MicroRNA-486.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100601

Methylmercury intoxication activates nitric oxide synthase in chick retinal cell culture
Provedor de dados: 56
Autores: Herculano,A.M.; Crespo-López,M.E.; Lima,S.M.A.; Picanço-Diniz,D.L.W.; Nascimento,J.L.M. Do.
The visual system is a potential target for methylmercury (MeHg) intoxication. Nevertheless, there are few studies about the cellular mechanisms of toxicity induced by MeHg in retinal cells. Various reports have indicated a critical role for nitric oxide synthase (NOS) activation in modulating MeHg neurotoxicity in cerebellar and cortical regions. The aim of the present study is to describe the effects of MeHg on cell viability and NOS activation in chick retinal cell cultures. For this purpose, primary cultures were prepared from 7-day-old chick embryos: retinas were aseptically dissected and dissociated and cells were grown at 37ºC for 7-8 days. Cultures were exposed to MeHg (10 µM, 100 µM, and 1 mM) for 2, 4, and 6 h. Cell viability was measured by MTT...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methylmercury; Chick retinal cell culture; Nitric oxide synthase; Cell viability; Neurotoxicity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300013

Enhanced anti-tumor efficacy of 5-aminolevulinic acid-gold nanoparticles-mediated photodynamic therapy in cutaneous squamous cell carcinoma cells
Provedor de dados: 56
Autores: Chi,Yu-fei; Qin,Jing-jing; Li,Zhi; Ge,Qin; Zeng,Wei-hui.
The objective of this study was to investigate whether the conjugation of gold nanoparticles (GNPs) to 5-aminolevulinic acid (5-ALA) could enhance the anti-tumor efficiency of photodynamic therapy (PDT) in epidermoid carcinoma cells. The mRNA and protein expression levels were determined by quantitative real-time PCR and western blot, respectively. Cell viability, apoptosis, invasion, and migration were determined by MTT assay, flow cytometry, transwell invasion assay, and migration assay, respectively. Singlet oxygen generation was detected by the singlet oxygen sensor green reagent assay. Our results showed that PDT with 5-ALA and GNPs-conjugated 5-ALA (5-ALA-GNPs) significantly suppressed cell viability, increased cell apoptosis and singlet oxygen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; 5-ALA-GNPs; Cell viability; Cell apoptosis; Cell invasion and migration; Cutaneous squamous cell carcinoma.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500608

ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
Provedor de dados: 65
Autores: Oliveira,Karen Maciel de; Binda,Nancy Scardua; Lavor,Mário Sérgio Lima; Silva,Carla Maria Osório; Rosado,Isabel Rodrigues; Taguchi,Tatiana Malagoli; Alves,Endrigo Gabellini Leonel; Melo,Marília Martins; Gomez,Marcus Vinícius; Melo,Eliane Gonçalves de.
This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MVIIA; Cone snail; Cell viability; Glutamate; Reactive oxygen species; Lipid peroxidation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150