Consulta: subjectFacets:"Cervical cancer"
Registros recuperados: 34
Data/hora: 10/06/2024 11:02:23

Association between cervical lesion grade and micronucleus frequency in the Papanicolaou test
Provedor de dados: Genetics and Molecular Biology
Autores: Bueno,Caroline Tanski; Silva,Cláudia Maria Dornelles da; Barcellos,Regina Bones; Silva,Juliana da; Santos,Carla Rossana dos; Menezes,João Evangelista Sampaio; Menezes,Honório Sampaio; Rossetti,Maria Lucia Rosa.
The aim of this study was to evaluate the association between the frequency of micronuclei (MN) and the cellular changes detected in the conventional Papanicolaou test. One hundred and seventy-four Papanicolaou test smears with cellular changes were examined. MN screening was done in cytopathological smears by counting 1,000 cervical cells in a light microscope. MN frequencies were significantly higher in the group with cellular changes compared to the control group (p &lt; 0.001). The mean MN frequencies were 0.95 ± 1.12 (mean ± SD) in the control group (n = 223), 2.98 ± 1.20 in individuals with atypical squamous cells of undetermined significance (ASC-US) (n = 50), 4.04 ± 1.45 in cervical intraepithelial neoplasia (CIN) I (n = 52), 5.97 ± 1.83 in CIN II...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; Cervical lesions; Micronucleus; Papanicolaou test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400004

Methylation in the promoter regions of WT1, NKX6-1 and DBC1 genes in cervical cancer tissues of Uygur women in Xinjiang
Provedor de dados: Genetics and Molecular Biology
Autores: Wu,Dan; Zhang,Jinli; Fan,Peiwen; Li,Hongtao; Li,Dongmei; Pan,Huan; He,Hongchang; Ren,Xianxian; Pan,Zhenzhen; Shao,Renfu; Pan,Zemin.
Abstract This study aimed to explore: 1) DNA methylation in the promoter regions of Wilms tumor gene 1 (WT1), NK6 transcription factor related locus 1 gene (NKX6-1) and Deleted in bladder cancer 1 (DBC1) gene in cervical cancer tissues of Uygur women in Xinjiang, and 2) the correlation of gene methylation with the infection of HPV16/18 viruses. We detected HPV16/18 infection in 43 normal cervical tissues, 30 cervical intraepithelial neoplasia lesions (CIN) and 48 cervical cancer tissues with polymerase chain reaction (PCR) method. Methylation in the promoter regions of the WT1, NKX6-1 and DBC1 genes in the above-mentioned tissues was measured by methylation-specific PCR (MSP) and cloning sequencing. The expression level of these three genes was measured by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene methylation; Gene expression; HPV16/18; Cervical cancer; Uygur women.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100009

Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
Provedor de dados: International Journal of Morphology
Autores: Ili,Carmen G; Brebi,Priscilla; Garcia,Patricia; Leal,Pamela; Lopez,Jaime; Tapia,Oscar; Letelier,Pablo; Weber,Helga; Castillo,Jonathan; Roa,Juan C.
Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected...
Tipo: Journal article
Palavras-chave: Apoptosis; Proliferation; Cervical cancer; SiRNA; C-FLIPL.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036

Expression of TRF2 and its prognostic relevance in advanced stage cervical cancer patients
Provedor de dados: Biological Research
Autores: Ozden,Sevgi; Tiber,Pinar Mega; Ozgen,Zerrin; Ozyurt,Hazan; Serakinci,Nedime; Orun,Oya.
BACKGROUND: Telomeres are protective caps consisted of specific tandem repeats (5&amp;#8242;-TTAGGG-3&amp;#8242;). Shortening of telomeres at each cell division is known as "mitotic clock" of the cells, which renders telomeres as important regulators of lifespan. TRF2 is one of the critical members of shelterin complex, which is a protein complex responsible from the preservation of cap structure, and loss or mutation of TRF2 results in DNA damage, senescence or apoptosis. Since cancer is frequently associated with aberrant cell cycle progression, defective DNA repair or apoptosis pathways, TRF2 could be one likely candidate for cancer therapy. Here we investigated the prognostic role of TRF2 levels in cervical cancer patients. Fold-induction rates were...
Tipo: Journal article
Palavras-chave: Cervical cancer; Radiotherapy; Telomere repeat-binding factors 2; B-cell lymphoma-extra large; Apoptosis.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100055

In vitro assessment of a computer-designed potential anticancer agent in cervical cancer cells
Provedor de dados: Biological Research
Autores: Visagie,Michelle Helen; Jaiswal,Seema Rummurat; Joubert,Anna Margaretha.
BACKGROUND: Computer-based technology is becoming increasingly essential in biological research where drug discovery programs start with the identification of suitable drug targets. 2-Methoxyestradiol (2ME2) is a 17ß-estradiol metabolite that induces apoptosis in various cancer cell lines including cervical cancer, breast cancer and multiple myeloma. Owing to 2ME2's poor in vivo bioavailability, our laboratory in silico-designed and subsequently synthesized a novel 2ME2 analogue, 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10),15-tetraen-17-ol (ESE-15-ol), using receptor- and ligand molecular modeling. In this study, the biological effects of ESE-15-ol (180 nM) and its parent molecule, 2ME2 (1 &amp;#956;M), were assessed on morphology and apoptosis induction in...
Tipo: Journal article
Palavras-chave: Morphology; Apoptosis; Cervical cancer; ESE-15-ol.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100043

The Role of Apollon Gene Silencing on Viablity and Radiosensitivity of Cervical Cancer Hela Cells
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Milani,Saeideh; Bandehpour,Mojgan; Sharifi,Zohreh; Kazemi,Bahram.
Cervical cancer is the second common cause of cancer deaths in women worldwide. Radioresistancy of cancer is a principal cause of treatment impairing. Inhibitor of apoptosis proteins (IAPs) widely block apoptosis against apoptotic stimuli, including current chemo- and radiation therapies. Apollon, a membrane of IAP, can support cells against apoptosis and is over expressed in some treatment-resistant cancer cells. The aim of this study was to evaluate the effects of apollon knockdown on induction of apoptosis and also its potential for enhancement of radiosensitvity on hela cells. plasmid encoding shRNA which has been confirmed its effect against apollon, transfected into hela cells. Consequent effects on the level of P53 , Bax and BAK analyzed by real...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apollon; Apoptosis; Radiosensitivity; Cervical cancer.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100320

Problems and pitfalls in vulvar and cervical cancer sentinel node scintigraphy
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Sinzinger,Helmut; Granegger,Susanne; Palumbo,Barbara; Palumbo,Renato.
After the introduction for penile cancer, the sentinel lymph node imaging is increasingly applied in various types of cancer. After the initial learning phase, 105 patients with vulvar and 24 with cervical cancer have been investigated. In vulvar cancer all the imaged sentinel nodes were discovered by the portable probe intraoperatively. No false negative sentinel node was observed. The most critical issue is the tracer application. Performed strictly intradermally, the sentinel node shows up immediately. Concomitant use of isosulfan blue dye did not improve the results and was stopped therefore. Similarly, more superficial (intra/subendothelial) application brings up better results as compared to deeper injection in cervical cancer patients. No false...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sentinel lymph node; Vulvar cancer; Cervical cancer; Scintigraphy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700007

TT virus in peripheral blood cells from patients with human papillomavirus (HPV): investigating association with cervical carcinoma
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Suzuki,Patrícia Sayuri; Oliveira,Karen Brajão de; Herrera,Ana Cristina do Amaral; Borelli,Sueli Donizete; Guembarovski,Roberta Losi; Oliveira,Carlos Eduardo Coral de; Oda,Julie Massayo Maeda; Ozawa,Patricia Midori Murobushi; Watanabe,Maria Angelica Ehara.
Torque Teno Virus (TTV) presence was investigated in peripheral blood of 117 brazilian women by nested polymerase chain reaction. TTV DNA was observed in 18.6% of healthy donors and in 24.32% Human Papillomavirus (HPV) patients. TTV presence was also investigated in the HPV positive group for comparison between the cervical cancer and noncancerous patients. TTV DNA prevalence was significantly higher among the HPV positive patients with cervical cancer (57.14%) than in HPV noncancerous patients (16.67%). Thus, the presence of TTV infection could be a risk factor for cancer development in the patients presenting HPV-TTV coinfection. Further studies are required to clarify the TTV influence in HPV pathogenesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TTV DNA; HPV; Cervical cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200010

The environmental cofactors in carcinogenesis in high risk HPV/HIV-positive women
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Paulo,Michele; Borges,Alex Bittencourt; Duarte,Geraldo; Quintana,Silvana Maria; Montes,Marlise Bonetti Agostinho; Toloi,Maria Regina Torqueti.
The objective of the present study was to assess the presence of human papilloma virus (HPV) in HIV-infected women, with comparison between the Papanicolaou cytologic technique and the molecular PCR technique, as well as to determine the type of HPV, to measure cellular immunocompetence and to identify the presence of risk factors for the acquisition of HPV infection. Thirty HIV-infected women were selected. Vaginal and endocervical samples were collected from 27 of them. The smears were examined by 3 experienced cytologists to diagnose the presence of HPV by the Papanicolaou technique and the results were compared to HPV detection and typing by PCR. HPV-infected patients were interviewed in order to identify the presence of risk factors for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; HIV-infected women; Cervical cancer; Carcinogenesis; Antiretroviral therapy.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200003

Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Entiauspe,Ludmila Gonçalves; Seixas,Fabiana Kömmling; Nunes,Emily Montosa; Rodrigues,Fernanda Martins; Dellagostin,Odir A.; Collares,Tiago; Silveira,Mariângela Freitas da.
Background:It is believed that Human Papillomavirus (HPV) and Human Immunodeficiency Virus coinfection contributes to increase the risk for cervical intraepithelial injuries. Several factors may contribute to cervical cancer (CC) development, including genetic variants such as TP53and MDM2 gene polymorphisms.Materials and methods:A hundred HIV-infected women were examined for HPV detection and its genotypes, as well as the frequencies of the SNPs Arg72Pro and SNP309 and their associations with CC risk factors. Nested Polymerase Chain Reaction (nPCR) was used for HPV detection and PCR-RFLP for TP53 and MDM2 SNP309 genotyping.Results:HPV DNA was detected in 68% of samples. A higher frequency of low-risk HPV genotypes (66.7%) was observed when compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV infection; HIV infection; Cervical cancer; Epidemiology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600643

Human papillomavirus infection in Rio de Janeiro, Brazil: a retrospective study
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Carvalho,M.O.O; Carestiato,F.N; Perdigão,P.H; Xavier,M.P.P.T; Silva,KC; Botelho,M.O; Oliveira,L.H.S.; Cavalcanti,S.M.B.
There is considerable data to support a central role for human papillomavirus (HPV) in the etiology of cervical cancer. More than a 100 HPV types have been described, and 40 have been isolated from benign and malignant genital lesions. Consequently, there is strong motivation to evaluate HPV testing for cervical cancer screening. Few studies concerning the natural history of HPV infection have been conducted in the state of Rio de Janeiro. We determined the prevalence of HPV types in female genital lesions by using Hybrid Capture Assay (HCA) and we retrospectively analyzed the course of HPV infection. Our sample included 788 women attended at Laboratórios Sérgio Franco. The average age of the participants was 29.6 years. HPV prevalence and cytological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; Cervical cancer; Rio de Janeiro; Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000500007

High-risk human papillomavirus and cervical lesions among women living with HIV/AIDS in Brazilian Amazon, Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Silva,Leila da; Miranda,Angélica; Batalha,Rosieny; Ferreira,Luiz; Santos,Mayara; Talhari,Sinésio.
ABSTRACT  OBJECTIVE:  The goal of this study was to determine the prevalence of human papillomavirus infection infection and cervical lesions and its associated factors among HIV infected women attending an AIDS clinic in Amazonas state, Brazil.  METHODS:  Cross-sectional study. Women attending an AIDS clinic in the city of Manaus between March and December 2011 for gynecological examination were invited to participate. Enrolled patients answered a standardized interview including demographical, behavioral, and clinical data. Additionally, patients underwent a gynecological evaluation with collection of cervical samples for cytological analysis and high-risk human papillomavirus infection hybrid capture. A blood sample was also obtained to determine CD4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; HIV; Cervical cancer; Brazilian Amazon.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600557

Interleukin-12 gene adjuvant increases the immunogenicity of virus-like particles of human papillomavirus type 16 regional variant strain
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Wei,Lanlan; Chu,Ming; Zhang,Qingmeng; Wang,Yan; Shang,Qinglong; Zhang,Yunyan; Zhang,Guangmei.
Objectives:To analyze the immunogenicity of virus-like particles (VLP) of human papillomavirus type 16 (HPV16) isolated in East China and the adjuvant potential of interleukin-12 (IL-12).Methods:The variant HPV16 L1VLP expressed in sf9 insect cells were purified with cesium chloride gradient centrifugation. BALB/c mice were vaccinated with VLP (L1N), VLP with Freund's adjuvant (L1A) or VLP with IL-12 recombinant plasmid (L1P). HPV16 VLP specific IgG and IFN-γ level in the serum were detected by ELISA, and the percentage of CD4+ and CD8+ in spleen cells was detected with flow cytometry.Results:The titers of serum IgG antibodies in vaccinated groups were higher than in negative control and the serum antibodies mainly recognized conformation-dependent HPV16...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; Human papillomavirus type 16; Virus-like particle; Cellular immunity; IL-12.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100065

Cervical human papillomavirus infection and persistence: a clinic-based study in the countryside from South Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Coser,Janaina; Boeira,Thaís da Rocha; Wolf,Jonas Michel; Cerbaro,Kamila; Simon,Daniel; Lunge,Vagner Ricardo.
Abstract Human papillomavirus (HPV) infection is common in sexually active women and viral persistence may cause intraepithelial lesions and eventually progress to cervical cancer (CC). The present study aimed to investigate epidemiological factors related to HPV infection and to evaluate viral persistence and CC precursor lesions frequencies in women from a city in the countryside of South Brazil. Three hundred women were recruited from a primary public health care clinic. The patients were interviewed and underwent sampling with cervical brushes for HPV-DNA detection/typing by a PCR-based assay and cytological analysis by Pap smear test. HPV was detected in 47 (15.7%) women. HPV infection was significantly associated with young age (&lt;30 years) and low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV prevalence; HPV persistence; Cervical cancer; Epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100061

Estrogen and progesterone receptors in human papilloma virus-related cervical neoplasia
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Coelho,F.R.G.; Prado,J.C.M.; Pereira Sobrinho,J.S.; Hamada,G.; Landman,G.; Pinto,C.A.; Nonogaki,S.; Villa,L.L.
Estrogen (ER) and progesterone (PR) receptors in the normal uterine cervix, cervical intraepithelial neoplasia and invasive carcinoma were studied in consecutive samples from Hospital do Câncer, São Paulo, between 1996 and 1997. Tissue was collected by removing a fragment of the tumoral area using a 5-mm diameter biopsy punch, followed by removal of a macroscopically normal area as close as possible from the tumor. Histopathological confirmation was obtained for all specimens analyzed. A total of 24 normal tissues, 17 cases of cervical intraepithelial neoplasia and 7 of invasive carcinomas were studied. The ER/PR ratio was determined by immunohistochemistry using monoclonal antibodies specific for each receptor. Adjacent tissue slides were submitted to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Estrogen receptor; Progesterone receptor; Cervical cancer; Cervical intraepithelial neoplasia; Human papillomavirus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100012

Overexpression of ezrin and galectin-3 as predictors of poor prognosis of cervical cancer
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Li,M.; Feng,Y.M.; Fang,S.Q.
The aim of this study was to explore the correlation of ezrin and galectin-3 expressions with prognosis in cervical cancer. The immunohistochemical method was applied to detect ezrin and galectin-3 expressions in normal cervix tissues (n=30), cervicitis tissues (n=28), cervical intraepithelial neoplasia (CIN) tissues (classified as I-III, n=89), and cervical carcinoma tissues (n=84). Follow-up was conducted for 5 to 78 months to analyze the correlation of protein expressions with prognosis. Ezrin and galectin-3 expressions in cervical cancer were significantly higher than in normal cervix, cervicitis and CIN (all P&lt;0.05), and expressions in CIN were significantly higher than in normal cervix and cervicitis (both P&lt;0.05). The expressions of ezrin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ezrin protein; Galectin-3 protein; Cervical cancer; Deep myometrial invasion; Lymph node metastasis; Prognosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400601

Production of L1 protein from different types of HPV in Pichia pastoris using an integrative vector
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Coimbra,E.C.; Gomes,F.B.; Campos,J.F.; D’arc,M.; Carvalho,J.C.; Mariz,F.C.; Jesus,A.L.S.; Stocco,R.C.; Beçak,W.; Freitas,A.C.
Human papillomavirus (HPV) infection is the most common sexually transmitted disease in the world and is related to the etiology of cervical cancer. The most common high-risk HPV types are 16 and 18; however, the second most prevalent type in the Midwestern region of Brazil is HPV-33. New vaccine strategies against HPV have shown that virus-like particles (VLP) of the major capsid protein (L1) induce efficient production of antibodies, which confer protection against the same viral type. The methylotrophic yeast Pichia pastoris is an efficient and inexpensive expression system for the production of high levels of heterologous proteins stably using a wild-type gene in combination with an integrative vector. It was recently demonstrated that P. pastoris can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Cervical cancer; Pichia pastoris; L1.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200003

PCR-RFLP assay as an option for primary HPV test
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Golfetto,L.; Alves,E.V.; Martins,T.R.; Sincero,T.C.M.; Castro,J.B.S.; Dannebrock,C.; Oliveira,J.G.; Levi,J.E.; Onofre,A.S.C.; Bazzo,M.L.
Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology and another sample was collected using BD SurePath™ kit for HPV tests. Eighty samples (24.6%) were positive for HPV gene by PCR-Multiplex and were then submitted to PCR-RFLP and PapilloCheck® microarray. There was a genotyping agreement in 71.25% (57/80) on at least one HPV type between PCR-RFLP and PapilloCheck® microarray. In 22 samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; HPV; Screening; PCR-RFLP; PapilloCheck® microarray.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500619

c-Myc protein expression is not an independent prognostic predictor in cervical squamous cell carcinoma
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Brenna,S.M.F.; Zeferino,L.C.; Pinto,G.A.; Souza,R.A.; Andrade,L.A.L.; Vassalo,J.; Martinez,E.Z.; Syrjänen,K.J.
The c-myc protein is known to regulate the cell cycle, and its down-regulation can lead to cell death by apoptosis. The role of c-myc protein as an independent prognostic determinant in cervical cancer is controversial. In the present study, a cohort of 220 Brazilian women (mean age 53.4 years) with FIGO stage I, II and III (21, 28 and 51%, respectively) cervical squamous cell carcinomas was analyzed for c-myc protein expression using immunohistochemistry. The disease-free survival and relapse-rate were analyzed using univariate (Kaplan-Meier) survival analysis for 116 women who completed the standard FIGO treatment and were followed up for 5 years. Positive c-myc staining was detected in 40% of carcinomas, 29% being grade 1, 9% grade 2, and 2% grade 3....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-myc; Oncogene; Cervical cancer; Prognosis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400003

Glycoprotein nonmetastatic melanoma protein B accelerates tumorigenesis of cervical cancer in vitro by regulating the Wnt/β-catenin pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Xu,Shuxiang; Fan,Yingying; Li,Dongping; Liu,Yan; Chen,Xu.
Cervical cancer is one of the most common cancers among women around the world. However, the underlying mechanism involved in cervical cancer progression is incompletely known. In the present study, we determined the role of glycoprotein nonmetastatic melanoma protein B (GPNMB) in tumorigenesis of cervical cancer. According to the GEO database, we found that GPNMB expression was significantly higher in cervical cancer than in normal cervix epithelium. A similar pattern was observed in GPNMB expression in cultured cervical cancer cells and normal cervical epithelial cells. Compared with the control, GPNMB knockdown significantly decreased the proliferation and migration capacity, but enhanced the apoptosis capacity of SiHa and HeLa cells. Additionally, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; GPNMB; Tumorigenesis; MMPs; Wnt/β-catenin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100607

SULF2 promotes tumorigenesis and inhibits apoptosis of cervical cancer cells through the ERK/AKT signaling pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Jiang,Tao; Chen,Zhao-Hui; Chen,Zhe; Tan,Dan.
The objective of this study was to explore the role of the SULF2-mediated ERK/AKT signaling pathway in cervical cancer. SULF2 expression was detected in tumor tissues and tumor-adjacent normal tissues from cervical cancer patients. HeLa cells were divided into six groups: control group, NC group, SULF2 siRNA group, SULF2 group, SULF2 + LY294002 group, and SULF2 + U0125 group. In each group, HeLa cells received the corresponding treatment, followed by measurement of the cellular biological characteristics and expression of the ERK/AKT signaling pathway. We also confirmed the effect of SULF2 in vivo using a xenograft model in nude mice. SULF2 was upregulated in cervical cancer tissues, which was specifically associated with the clinical stage, histological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SULF2; Cervical cancer; ERK/AKT; Proliferation; Apoptosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200608

p16INK4 expression in precursor lesions of squamous cell cervical cancer related to the presence of HPV-DNA
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Godoy,A.E.G.; Mandelli,J.; Oliveira,F.H.; Calegari,S.; Moura,L.B.; Serafini,E.P.
The purpose of the present study was to identify the expression of p16INK4 in cervical cancer precursor lesions by immunohistochemistry and to correlate it with lesion grade and presence of human papillomavirus (HPV) infection. Cervical specimens from 144 women seen consecutively at the gynecology outpatient clinic of our institution from December 2003 to May 2005 were analyzed by cytopathology, histopathology, polymerase chain reaction for HPV-DNA, and p16INK4 immunostaining. Histologically normal biopsies, HPV-DNA negative by polymerase chain reaction, were used as control. HPV-DNA prevalence, including the control group, was 68.1% and the prevalence of p16INK4 expression was 55.0%. The percentage of cells stained by p16INK4 ranged from 10 to 100%, both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P16INK4; Cervical cancer; Human papillomavirus; Immunohistochemistry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700006

lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Song,Linlin; Wang,Linlin; Pan,Xiaoli; Yang,Caihong.
Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is one kind of cytoplasmic long non-coding RNA (lncRNA), which has been demonstrated to play a critical function in multiple cancers. However, the detailed mechanism of OIP5-AS1 in the regulation of cervical cancer progression is still obscure. Here, we demonstrated that lncRNA OIP5-AS1 was upregulated in cervical cancer and was correlated with poor prognosis by bioinformatics studies. OIP5-AS1 depletion inhibited cell proliferation and promoted cell apoptosis in cervical cancer cells. Furthermore, we clarified that ROCK1 was the downstream effector of OIP5-AS1 and OIP5-AS1 acted as a molecular sponge of miR-143-3p. Finally, we verified that OIP5-AS1 exerted its function in the regulation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA; OIP5-AS1; Cervical cancer; MiR-143-3p; ROCK1.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100608

Epidemiological and functional implications of molecular variants of human papillomavirus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sichero,L.; Villa,L.L.
Human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the L1 gene. Comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. In addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. Until the present, little has been published concerning the functional significance of molecular variants. It has been shown that nucleotide variability within the long control region leads to differences in the binding affinity of some cellular transcriptional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; DNA sequencing; Oncogenic potential; Cervical cancer; Immunity; Epidemiology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600002

The contribution of Latin American research to HPV epidemiology and natural history knowledge
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sichero,L.; Picconi,M.A.; Villa,L.L.
Our aim was to review the major contributions of studies conducted in different Latin American (LA) countries to the field of human papillomavirus (HPV) epidemiology, natural history, risk of disease, and prevention strategies, mainly in the uterine cervix. Although cytological screening is established in several countries in LA, incidence and mortality rates from cervical cancer (CC) are still extremely high. Finally, data from large cohort studies conducted in LA countries provided seminal data to propose primary and secondary prevention modalities: the HPV vaccine has been introduced in the national immunization programs of several LA countries and multiple screening experiences using HPV testing are under evaluation in the region.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; Latin America; Immunization; Cervical cancer; Natural history; Screening.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200302

Genetic susceptibility to HPV infection and cervical cancer
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Maciag,P.C.; Villa,L.L.
Squamous cell carcinoma of the cervix (SCCC) is one of the leading causes of death in developing countries. Infection with high-risk human papillomavirus (HPV) is the major risk factor to develop malignant lesions in the cervix. Polymorphisms of the MHC and p53 genes seem to influence the outcome of HPV infection and progression to SCCC, although controversial data have been reported. MHC are highly polymorphic genes that encode molecules involved in antigen presentation, playing a key role in immune regulation, while p53 is a tumor suppressor gene that regulates cell proliferation. The HPV E6 protein from high-risk types binds p53 and mediates its degradation by the ubiquitin pathway. The role of these polymorphisms in genetic susceptibility to HPV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Cervical cancer; HLA; P53; Polymorphism.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700017

The effect of performing corrections on reported uterine cancer mortality data in the city of São Paulo
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Antunes,J.L.F.; Wünsch-Filho,V.
Reports of uterine cancer deaths that do not specify the subsite of the tumor threaten the quality of the epidemiologic appraisal of corpus and cervix uteri cancer mortality. The present study assessed the impact of correcting the estimated corpus and cervix uteri cancer mortality in the city of São Paulo, Brazil. The epidemiologic assessment of death rates comprised the estimation of magnitudes, trends (1980-2003), and area-level distribution based on three strategies: i) using uncorrected death certificate information; ii) correcting estimates of corpus and cervix uteri mortality by fully reallocating unspecified deaths to either one of these categories, and iii) partially correcting specified estimates by maintaining as unspecified a fraction of deaths...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uterine cancer; Cervical cancer; Mortality; Statistical models; Socioeconomic factors; São Paulo.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800012

Radiotherapy modulates expression of EGFR, ERCC1 and p53 in cervical cancer
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: de Almeida,V.H.; de Melo,A.C.; Meira,D.D.; Pires,A.C.; Nogueira-Rodrigues,A.; Pimenta-Inada,H.K.; Alves,F.G.; Moralez,G.; Thiago,L.S.; Ferreira,C.G.; Sternberg,C.
Cervical cancer is a public health problem and the molecular mechanisms underlying radioresistance are still poorly understood. Here, we evaluated the modulation of key molecules involved in cell proliferation, cell cycle and DNA repair in cervical cancer cell lines (CASKI and C33A) and in malignant tissues biopsied from 10 patients before and after radiotherapy. The expression patterns of epidermal growth factor receptor (EGFR), excision repair cross-complementation group 1 (ERCC1) and p53 were evaluated in cancer cell lines by quantitative PCR and western blotting, and in human malignant tissues by immunohistochemistry. The mutation status of TP53 gene was evaluated by direct sequencing. Among cell lines, absent or weak modulations of EGFR, ERCC1 and p53...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; Radiotherapy; Epidermal growth factor receptor (EGFR); P53; Excision repair cross-complementation group 1 (ERCC1).
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100608

Analysis of the application values of different combination schemes of liquid-based cytology and high-risk human papilloma virus test in the screening of high-grade cervical lesions
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wang,Jian.
The aim of this study was to explore the value of different combination schemes of liquid-based cytology (LBC) and high-risk human papilloma virus (HPV) test in the screening of high-grade (≥CIN 2) cervical lesions. From 5727 women who had undergone examinations with LBC and high-risk HPV test, 1884 patients with positive results of either or both LBC and HPV test were included in this study and underwent cervical biopsy. Based on the pathological examination results, comparisons of the assessment indicators of all diagnostic tests were made, and the application values of LBC and high-risk HPV test and different combination schemes of the two in the screening of high-grade (≥CIN II) cervical lesions were estimated. Compared with the single test method, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liquid-based cytology; Human papilloma virus; Cervical cancer; ROC curve; High-grade.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100601

High incidence of oncogenic HPV genotypes found in women from Southern Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Entiauspe,Ludmila G; Silveira,Mariângela; Nunes,Emily M; Basgalupp,Suélen P; Stauffert,Dulce; Dellagostini,Odir A.; Collares,Tiago; Seixas,Fabiana K.
Oncogenic HPV genotypes are strongly associated with premalignant and malignant cervical lesion. The purpose was to determine human papillomavirus (HPV) prevalence and genotypes, and to estimate cervical cancer risk factor associations. Cervical samples were obtained from 251 women seeking gynecological care at the Pelotas School of Medicine Clinic. This is a cross-sectional study. HPV-DNA was amplified by nested-PCR using MY09/11 and GP5/6 primers, and the sequencing was used for genotyping. Sociodemographic and behavioral risk factors were obtained by closed questionnaire, and its relationship to HPV infection prevalence were analyzed. Statistical analyses were performed using SPSS 16.0 software, and differences were considered significant at p &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; SDT; Molecular epidemiology; Cervical cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200043

Human papillomavirus infection in women attended at a cervical cancer screening service in Natal, Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Fernandes,Thales Allyrio Araújo de Medeiros; Meissner,Rosely de Vasconcellos; Bezerra,Laelson Freire; Azevedo,Paulo Roberto Medeiros de; Fernandes,José Veríssimo.
We analyzed cervical specimens of 202 women, aged 15 to 64 years, attended at Luis Antonio Hospital, Natal, Brazil, to determine the prevalence of HPV and identify the more frequent genotypes and risk factors for HPV infection in women attended at a cervical cancer screening service. Two specimens were collected from each patient: one for cytological examination and the other to detect HPV DNA by PCR, and typing by dot blot hybridization. A total of 54.5% of the sample had normal cytology and 45.5% had cytological alterations. HPV was detected in 24.5% of the cytologically normal women and in 59.8% of those with altered cytology. Both single and double HPV infection increased the likelihood of cytological alterations. Thirteen types of HPV were identified,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Cervical cancer; Epidemiology; Prevalence; Risk factors.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300031

Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA
Provedor de dados: Brazilian Journal of Microbiology
Autores: Santos,Carla R.; Franciscatto,Laura G.; Barcellos,Regina B.; Almeida,Sabrina E. M; Rossetti,Maria Lucia R.
This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; Cervical cancer; FTA elute; Molecular detection.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100047

Correlation between ebv co-infection and HPV16 genome integrity in Tunisian cervical cancer patients
Provedor de dados: Brazilian Journal of Microbiology
Autores: Kahla,Saloua; Oueslati,Sarra; Achour,Mongia; Kochbati,Lotfi; Chanoufi,Mohamed Badis; Maalej,Mongi; Oueslati,Ridha.
Infection with high risk Human papillomavirus (HR-HPV) is necessary but not sufficient to cause cervical carcinoma. This study explored whether multiple HR-HPV or coinfection with Epstein-Barr virus (EBV) influence the integration status of HPV16 genome. The presence and typing of HPV in a series of 125 cervical specimens were assessed by polymerase chain reaction (PCR) using the specific primers for the HPV L1 region. As for EBV infection, the viral EBNA1 gene was used for its detection through PCR amplification. Disruption of the HPV E2 gene was assessed by amplification of the entire E2 gene with single set of primers, while E2 transcripts were evaluated by a reverse transcription PCR method (RT-PCR). The overall prevalence of HPVDNA was of 81.8% in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; Epstein-Barr virus; E2 gene; E2 mRNA; High risk human papillomaviruses.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200039

Antineoplastic potential of the aqueous crude extract of Eugenia uniflora L. in human cervical cancer
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Núñez,Jisette González; Pinheiro,Jordânia dos Santos; Silveira,Gabriel Fernandes; Beckenkamp,Aline; Buffon,Andréia; Bruno,Alessandra Nejar.
Abstract Considering the high prevalence of human cervical cancer and the adverse effects of the available treatments, it is important to develop studies involving plants. Eugenia uniflora L. is a Brazilian native plant widely used in folk medicine and some biological effects have already been described. In this study, we investigated the biologicals effects of the aqueous crude extract of E. uniflora leaves in relation to the viability of human cervical cancer cells (SiHa), non-tumorigenic cells HaCaT and human lymphocytes. Our results demonstrated that different concentrations of E. uniflora’s extract significantly inhibited the viability of the Siha cell line at 24, 48 and 72 hours of treatment, but did not induce significant changes in the HaCat cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eugenia uniflora L./effects/antitumoral; Pitangueira; SiHa; HaCat; Plant extract/antitumoral; Cervical cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200618