Consulta: subjectFacets:"Chemoprevention"
Registros recuperados: 9
Data/hora: 05/06/2024 06:36:04

Assessment of genetic integrity, splenic phagocytosis and cell death potential of (Z)-4-((1,5-dimethyl-3-oxo-2-phenyl-2,3dihydro-1H-pyrazol-4-yl) amino)-4-oxobut-2-enoic acid and its effect when combined with commercial chemotherapeutics
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Rodrigo Juliano; da Cruz Leite Santos,Naiara; Pesarini,João Renato; de Oliveira,Beatriz Carneiro; Berno,Claudia Rodrigues; de Araújo,Flávio Henrique Souza; da Silveira,Ingridhy Ostaciana Maia Freitas; Nascimento,Raquel Oliveira; Brochado Antoniolli-Silva,Andréia Conceição Milan; Duenhas Monreal,Antônio Carlos; Beatriz,Adilson; de Lima,Dênis Pires; da Silva Gomes,Roberto.
Abstract The increased incidence of cancer and its high treatment costs have encouraged the search for new compounds to be used in adjuvant therapies for this disease. This study discloses the synthesis of (Z)-4-((1,5-dimethyl-3-oxo-2-phenyl-2,3dihydro-1H-pyrazol-4-yl) amino)-4-oxobut-2-enoic acid (IR-01) and evaluates not only the action of this compound on genetic integrity, increase in splenic phagocytosis and induction of cell death but also its effects in combination with the commercial chemotherapeutic agents doxorubicin, cisplatin and cyclophosphamide. IR-01 was designed and synthesized based on two multifunctionalyzed structural fragments: 4-aminoantipyrine, an active dipyrone metabolite, described as an antioxidant and anti-inflammatory agent; and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Splenic phagocytosis; Comet assay; Micronucleus test; Cell death; Chemoprevention.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100154

Effects of β-glucan polysaccharide revealed by the dominant lethal assay and micronucleus assays, and reproductive performance of male mice exposed to cyclophosphamide
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Rodrigo Juliano; Pesarini,João Renato; Salles,Maria José Sparça; Kanno,Tatiane Yumi Nakamura; Lourenço,Ana Carolina dos Santos; Leite,Véssia da Silva; Silva,Ariane Fernanda da; Matiazi,Hevenilton José; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
β-glucan is a well-known polysaccharide for its chemopreventive effect. This study aimed to evaluate the chemopreventive ability of β-glucan in somatic and germ cells through the dominant lethal and micronucleus assays, and its influence on the reproductive performance of male mice exposed to cyclophosphamide. The results indicate that β-glucan is capable of preventing changes in DNA in both germ cells and somatic ones. Changes in germ cells were evaluated by the dominant lethal assay and showed damage reduction percentages of 46.46% and 43.79% for the doses of 100 and 150 mg/kg. For the somatic changes, evaluated by micronucleus assay in peripheral blood cells in the first week of treatment, damage reduction percentages from 80.63-116.32% were found. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation; Post-implantation losses; Chemoprevention; Micronucleus; Nulliparous females.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100017

Evaluation of antimutagenic and cytotoxic activity of skin secretion extract of Rhinella marina and Rhaebo guttatus (Anura, Bufonidae)
Provedor de dados: Acta Amazonica
Autores: OLIVEIRA,Angellica Fernandes de; CASTOLDI,Lindsey; VIEIRA JUNIOR,Gerardo Magela; MONÇÃO FILHO,Evaldo dos Santos; CHAVES,Mariana Helena; RODRIGUES,Domingos de Jesus; SUGUI,Marina Mariko.
ABSTRACT The skin secretion from toads of the Bufonidae family has great potential in the search for new active compounds to be used as drug candidates in treating some diseases, among them cancer. In this context, this study aimed to evaluate the cytotoxic and antimutagenic activity of the parotoid gland secretion extracts of Rhinella marina and Rhaebo guttatus, as well as biochemically analyze transaminases and serum creatinine for liver and renal damage, respectively. Cytotoxicity was performed by the colorimetric method based on MTT (3- [4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide) with different concentrations of the extracts in Walker or splenic tumor cell cultures from rats and mice. The micronucleus test was performed with male...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Bioprospecting; Micronuclei; Chemoprevention.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000200145

In vitro antioxidant, antitumor and leishmanicidal activity of riparin A, an analog of the Amazon alkamides from Aniba riparia (Lauraceae)
Provedor de dados: Acta Amazonica
Autores: ARAÚJO,Éverton José Ferreira de; LIMA,Layana Karine Farias; SILVA,Oskar Almeida; JÚNIOR,Luís Mário REZENDE; GUTIERREZ,Stanley Juan Chavez; CARVALHO,Fernando Aécio de Amorim; LIMA,Francisco das Chagas Alves; PESSOA,Cláudia; FREITAS,Rivelilson Mendes de; FERREIRA,Paulo Michel Pinheiro.
ABSTRACT Aniba riparia (Lauraceae) is an important medicinal plant found in the Amazon region and presents alkaloids of the type alkamide known as riparins. Riparin A is structurally represented as the fundamental core of all Amazon riparins. This work aimed to assess the in vitro antioxidant, antitumor and antileishmanial effects of riparin A. Riparin A presented weak antioxidant capacity by tecniques of DPPH• (EC50 of 296.2 μg mL-1) and ABTS•+ (EC50 of 450.1 μg mL-1), showed moderate activity against colon carcinoma (HCT-116: IC50 of 21.7 μg mL-1) and leishmanicidal activity on promastigotes of L. amazonensis (IC50 of 307.0 ± 79.6, 193.7 ± 44.3 and 81.8 ± 11.2 μg mL-1, respectively, after 24, 48 and 72 h of incubation). Then, in addition to its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioprospecting; Chemoprevention; Cytotoxicity; Antiparasitic drug.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000300309

Brown Flaxseed Prevents DNA Damage Induced by 1,2-Dimethylhydrazine in a Pre-Clinical Model
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Pamplona-Silva,Maria Tereza; Morandi,Walter Vinicius; Bernardi,Luana; Tura,Bethânia Borges; Oliveira,Diego Duarte Marques de; Antoniolli-Silva,Andreia Conceição Milan Brochado; Mauro,Mariana de Oliveira; Oliveira,Rodrigo.
ABSTRACT The study evaluated the effects of brown flaxseed supplementation in natura on the prevention of DNA damage induced by 1,2-dimethylhydrazine (DMH) in vivo. The experimental groups were Negative and Positive Controls and the protocols of Pre-treatment, Simultaneous, Post-treatment, Pre+continuous in relation to the supplementation of brown flaxseed and administration with carcinogenic compound. The results showed that brown flaxseed supplementation does not cause genomic and genetic damage. In addition, brown flaxseed showed a chemopreventive food that reduced the damages assessed by the comet assay up to 94.07x and the damages assessed by the micronucleus assay up to 91.88x. Brown flaxseed supplementation also increased the frequency of monocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flaxseed; Antigenotoxicity; Chemoprevention; Mice.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100316

Water extracts of cabbage and kale inhibit ex vivo H2O2-induced DNA damage but not rat hepatocarcinogenesis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Horst,M.A.; Ong,T.P.; Jordão Jr.,A.A.; Vannucchi,H.; Moreno,F.S.; Lajolo,F.M.
The chemopreventive potential of water extracts of the Brassica vegetables cabbage and kale was evaluated by administering their aqueous extracts in drinking water ad libitum to Wistar rats submitted to Ito’s hepatocarcinogenesis model (CB group and K group, respectively - 14 rats per group). Animals submitted to this same model and treated with water were used as controls (W group - 15 rats). Treatment with the vegetable extracts did not inhibit (P &gt; 0.05) placental glutathione S-transferase-positive preneoplastic lesions (PNL). The number of apoptotic bodies did not differ (P &gt; 0.05) among the experimental groups. Ex vivo hydrogen peroxide treatment of rat livers resulted in lower (P &lt; 0.05) DNA strand breakage in cabbage- (107.6 ± 7.8 µm) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemoprevention; Hepatocarcinogenesis; Diethylnitrosamine; Brassica vegetables; DNA damage.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300004

Efficacy of geraniol but not of β-ionone or their combination for the chemoprevention of rat colon carcinogenesis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Vieira,A.; Heidor,R.; Cardozo,M.T.; Scolastici,C.; Purgatto,E.; Shiga,T.M.; Barbisan,L.F.; Ong,T.P.; Moreno,F.S.
β-ionone (βI), a cyclic isoprenoid, and geraniol (GO), an acyclic monoterpene, represent a promising class of dietary chemopreventive agents against cancer, whose combination could result in synergistic anticarcinogenic effects. The chemopreventive activities of βI and GO were evaluated individually or in combination during colon carcinogenesis induced by dimethylhydrazine in 48 3-week-old male Wistar rats (12 per group) weighing 40-50 g. Animals were treated for 9 consecutive weeks with βI (16 mg/100 g body weight), GO (25 mg/100 g body weight), βI combined with GO or corn oil (control). Number of total aberrant crypt foci (ACF) and of ACF ≥4 crypts in the distal colon was significantly lower in the GO group (66 ± 13 and 9 ± 2, respectively) compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colon; Carcinogenesis; Chemoprevention; Β-ionone; Geraniol; Isoprenoid; Acyclic monoterpene.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600007

Punica granatum L. protects mice against hexavalent chromium-induced genotoxicity
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Ávila,Renato Ivan de; Guerra,Meirielle Teixeira; Borges,Kamilla de Almeida Souza; Vieira,Marcelo de Sousa; Oliveira Júnior,Luiz Marcos de; Furtado,Humberto; Mota,Mariana Flavia da; Arruda,Andréa Fernandes; Valadares,Marize Campos.
This study investigated the chemoprotective effects of Punica granatum L. (Punicaceae) fruits alcoholic extract (PGE) on mice exposed to hexavalent chromium [Cr(VI)]. Animals were pretreated with PGE (25, 50 or 75 mg/kg/day) for 10 days and subsequently exposed to a sub-lethal dose of Cr(VI) (30 mg/kg). The frequency of micronucleated polychromatic erythrocytes in the bone marrow was investigated and the Cr(VI) levels were measured in the kidneys, liver and plasm. For the survival analysis, mice were previously treated with PGE for 10 days and exposed to a single lethal dose of Cr(VI) (50 mg/kg). Exposure to a sub-lethal dose of Cr(VI) induced a significant increase in the frequency of micronucleated cells. However, the prophylactic treatment with PGE led...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemoprevention; Hexavalent chromium/induced genotoxicity; Oxidative damage; Pomegranate/chemoprotective effects; Punica granatum/chemoprotective effects; Punica granatum/pharmacognosy; Punicaceae/chemoprotective effects; Punicaceae/pharmacognosy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400008

Dual effect of Algerian propolis on lung cancer: antitumor and chemopreventive effects involving antioxidant activity
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Brihoum,Hadjer; Maiza,Mhamed; Sahali,Hafida; Boulmeltout,Malika; Barratt,Gillian; Benguedouar,Lamia; Lahouel,Mesbah.
Abstract The purpose of our study was to divulge the antiproliferative effect of an ethanolic extract of Algerian propolis (EEP) in the human lung adenocarcinoma cell line (A549) and reveal the chemopreventive role against benzo(a)pyrene-induced lung carcinogenesis in albino Wistar rats. Cytotoxicity of EEP was evaluated using the MTT assay and cell adhesion in A549 cells. Moreover, rats were given 25 mg/kg of propolis for 5 days before induction of experimental lung cancer by a single intraperitoneal dose of 200 mg/kg benzo(a)pyrene. Body weight, lung weight, lipid peroxidation, marker enzymes, and enzymatic and non-enzymatic antioxidants were estimated. The EEP demonstrated an inhibitory effect on proliferation of A549 at 24 and 72 hours in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Algerian propolis/effects; Benzo(a)pyrene; Chemoprevention; Lung cancer; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100616