Consulta: subjectFacets:"Chromosome 17"
Registros recuperados: 3
Data/hora: 17/06/2024 14:46:32

Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas
Provedor de dados: 74
Autores: Veiga,Luciana CS; Bérgamo,Nádia A; Kowalski,Luiz Paulo; Rogatto,Silvia R.
Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FISH; Chromosomal aberrations; Head and neck cancer; Chromosome 17.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200003

Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
Provedor de dados: 74
Autores: Otero,Luize; Cavalcanti Júnior,Geraldo Barroso; Klumb,Claudete Esteves; Scheiner,Marcos Antonio Mauricio; Magluta,Eliane Pereira Simões; Fernandez,Teresa de Souza; Silva,Maria Luiza Macedo; Pires,Virgínia; Andrade,Gabriela Vasconcelos; Maia,Raquel Ciuvalschi; Tabak,Daniel.
chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic myeloid leukemia; Chromosome 17; TP53 gene; P53 protein.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100007

TP53 mutations and loss of heterozygosity of chromosome 17 in colorectal tumors
Provedor de dados: 54
Autores: Yamamoto,Lidia; Lopes,Ademar A.; Harb-Gama,Angelito; Nagai,Maria A.
The incidence of TP53 point mutations and loss of heterozygosity (LOH) of chromosome 17 in colorectal tumors was determined in a group of Brazilian patients. We screened DNA samples from tumors and distal normal mucosa of 39 patients with colorectal cancer, for TP53 mutations by PCR-SSCP (single-strand conformation polymorphism) analysis. Chromosome 17 LOH was investigated using six PCR-based polymorphic markers and one VNTR probe. TP53 mutations were demonstrated in 15/39 of the cases. Mutations were distributed among all exons examined (five to eight), the majority of them being G/C to A/T transitions. LOH of chromosome 17p and 17q was detected in 70 and 46% of the tumors, respectively. There was a significant association between TP53 mutations and LOH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TP53 mutations; Heterozygosity; Chromosome 17; Colorectal tumors.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400022