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Exoproteolytic activity determined by flow injection analysis: its potential importance for bacterial growth in coastal marine ponds
Provedor de dados: 5
Autores: Delmas, Daniel; Legrand, Catherine; Bechemin, Christian; Collinot, Cecile.
The measurement of the fluorescent 4-methyl-7-coumarinylamine released from the hydrolysis of a non-fluorescent peptide model substrate by exoproteolytic enzymes has been adapted to flow injection analysis (FIA). FIA allows samples to be processed very quickly (less than 2 min. for triplicate determinations) with good sensitivity (&lt; 0.1 muM) and reproducibility (relative standard deviation &lt; 3 % at the 0.2 muM level). In a coastal marine pond, exoproteolytic activity was closely related to bacterioplankton biomass. The high activity measured in pond water (maximum velocity: V(M) almost-equal-to 1.46 to 2.54 muM.h-1) emphasizes the importance of dissolved protein hydrolysis for bacterial growth and for dissolved organic nitrogen cycling. The mean...
Tipo: Text
Palavras-chave: Activité exoprotéolytique; Protéine dissoute; Analyse en flux continu; Bactérioplancton; Marais maritime; Flow injection analysis; Exoproteolytic activity; Dissolved protein; Bacterioplankton; Coastal marine pond.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00196/30756/29163.pdf