Consulta: subjectFacets:"Colony PCR"
Registros recuperados: 3
Data/hora: 02/06/2024 02:19:11

A simple negative selection method to identify adenovirus recombinants using colony PCR
Provedor de dados: Electronic Journal of Biotechnology
Autores: Zhao,Yongliang; Dong,Zongming; Li,Tengfei; Huang,Gang.
Background: The AdEasy system is a fast-track system for generating recombinant adenoviruses using the efficient homologous recombination machinery between shuttle and adenovirus backbone plasmids in Escherichia coli BJ5183 cells. The key step is homologous recombination in BJ5183 cells, which is driven by RecA activity. However, culture time is stringently limited to reduce the damage to recombinant plasmids by RecA activity. Therefore, rapid identification of recombinant adenoviruses within the limited time-period is critical. Results: We developed a simple negative selection method to identify recombinant adenoviruses using colony PCR, which improves the efficiency of adenovirus recombination screening and packaging. Conclusions: The negative selection...
Tipo: Journal article
Palavras-chave: Adenovirus; AdEasy; Colony PCR; Homologous recombination; RecA.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100008

A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
Provedor de dados: Electronic Journal of Biotechnology
Autores: Revelo Romo,Dolly Margot; Grosso,María Victoria; Moreno Solano,Nubia Carmenza; Montoya Castaño,Dolly.
A molecular approach was used for selecting polyhydroxyalcanoate (PHA)-accumulating potential Gram-negative bacteria from different genera by colony polymerase chain reaction (PCR). Three degenerate primers were designed for amplifying a fragment from PHA synthase gene (phaC) (Class I), phaC1 and phaC2 (Class II) genes for detecting PHA-producing bacteria. Thirty-four out of 55 bacterial strains from the old collection selected using Sudan black B staining were phaC+. PCR was used for directly selecting 35 new collection bacterial strains; these strains were phaC+ and their ability to produce PHA was confirmed by Sudan black B staining. Four specific primers were designed on genes of Class II PHA biosynthesis operon. These primers were used for evaluating...
Tipo: Journal article
Palavras-chave: 16S rDNA; Colony PCR; Degenerate primers; PHA synthase; Polyhydroxyalcanoate; Seminested PCR.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300002

Isolation and Identification of Novel IndigenousBacterial Strain as a Low Cost Pectinase Source
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Bibi,Nowshad; Ali,Sikander; Tabassum,Romana.
ABSTRACT The present study was concerned with the searching of novel bacterial cultures from different samples for the lab scale production of pectinase. Keeping in view the increasing demand of pectinase specially in Faisalabad, an industrial city of Pakistan, isolation of new hyper producer bacterial strains locally is an easy and cheap way of getting the desirable products at low cost. Therefore, isolation of new strains for industrial enzyme production has been and will be remained a part of research every time. This method alone can also provide raw material for further research such as enzyme engineering or molecular directed evolution. For the identification of hyper producer strain colony PCR was done for 16S rRNA analysis. Reason to use the 16S...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indigenous; Low cost; Colony PCR; Bacillus licheniformis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100424