Consulta: subjectFacets:"Confocal laser scanning microscopy"
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Data/hora: 09/06/2024 02:57:49

The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction
Provedor de dados: 56
Autores: de-Souza,W.; de-Carvalho,T.U.; de-Melo,E.T.; Soares,C.P.; Coimbra,E.S.; Rosestolato,C.T.; Ferreira,S.R.; Vieira,M.
In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein phosphorylation, revealed by immunocytochemistry, and sites of actin assembly, revealed by the use of labeled phaloidin. The results obtained show that proteins localized in the interaction sites are phosphorylated. The process of formation of the parasitophorous vacuole was monitored by labeling the host cell surface with fluorescent probes for lipids (PKH26), proteins (DTAF) and sialic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Confocal laser scanning microscopy; Host cell-parasite interaction; Trypanosoma cruzi; Toxoplasma gondii.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100015

In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China
Provedor de dados: 58
Autores: Dawei,Guo; Liping,Wang; Chengping,Lu.
Streptococcus suis is a swine pathogen and also a zoonotic agent. The formation of bioﬁlms allows S. suis to become persistent colonizers and resist clearance by the host immune system and antibiotics. In this study, biofilm forming potentials of various S. suis strains were characterized by confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and tissue culture plates stained with crystal violet. In addition, the effects of five antimicrobial agents on biofilm formation were assayed in this study. S. suis produced biofilms on smooth and rough surface. The nutritional contents including glucose and NaCl in the growth medium modulated biofilm formation. There was a significant difference in their biofilm-forming ability among all 46...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Streptococcus suis; Scanning electron microscopy; Confocal laser scanning microscopy; Microplate assay; Antimicrobial agents.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300021