Consulta: subjectFacets:"Conventional PCR"
Registros recuperados: 4
Data/hora: 10/06/2024 10:29:41

A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae , Vibrio parahaemolyticus and Vibrio vulnificus in seafoods
Provedor de dados: 5
Autores: Hartnell, R. E.; Stockley, L.; Keay, W.; Rosec, J. -p.; Hervio-heath, Dominique; Van Den Berg, H.; Leoni, F.; Ottaviani, D.; Henigman, U.; Denayer, S.; Serbruyns, B.; Georgsson, F.; Krumova-valcheva, G.; Gyurova, E.; Blanco, C.; Copin, S.; Strauch, E.; Wieczorek, K.; Lopatek, M.; Britova, A.; Hardouin, G.; Lombard, B.; Veld, P. In'T; Leclercq, A.; Baker-austin, C.
Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus) was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also...
Tipo: Text
Palavras-chave: Vibrios; Seafood; Prawns; Oysters; Biochemical methods; Real-time PCR; Conventional PCR.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00425/53680/54525.pdf

Pathological, immunohistochemical, and molecular findings in commercial laying hens and in backyard chickens naturally infected with the infectious laryngotracheitis virus
Provedor de dados: 89
Autores: Preis,IS; Fiúza,ATL; Silva,CC; Braga,JFV; Couto,RM; Martins,NR da S; Ecco,R.
Seventy-eight chickens from a very high poultry density (approximately eight million) region and twelve backyard chickens from neighboring areas were analyzed by histopathology and additional techniques for the presence of the infectious laryngotracheitis virus. The virus distribution was determined in different tissues using immunohistochemistry (IHC) and polymerase chain reaction (PCR). The disease was histopathologically diagnosed in 41.0% (32/78) of the commercial layers. Lesions were mainly characterized by syncytial cells with eosinophilic intranuclear inclusion body formed from the hyperplastic epithelium of the upper respiratory tract, primary and secondary bronchi, and conjunctiva. IHC showed 70% (21/30) positive signal in the larynx/trachea and,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian infectious laryngotracheitis; Conventional PCR; Histopathology; Immunohistochemistry; Laying hen.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000400004

Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women
Provedor de dados: 55
Autores: Ferreira,Michele Berger; de-Paris,Fernanda; Paiva,Rodrigo Minuto; Nunes,Luciana de Souza.
ABSTRACT Group B Streptococcus is a causative agent of invasive neonatal infections. Maternal colonization by Streptococcus agalactiae is a necessary condition for vertical transmission, with efficient screening of pregnant women playing an essential role in the prevention of neonatal infections. In this study, we aimed to compare the performance of conventional polymerase chain reaction and real-time PCR assays as screening methods for S. agalactiae in pregnant women against the microbiological culture method considered as the gold-standard. A total of 130 samples from pregnant women were analyzed for sensitivity, specificity, positive predictive value, and negative predictive value. Statistical analysis was performed using the SPSS software, version...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus agalactiae; Pregnant women; Screening; Conventional PCR; Real-time PCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600449

Principles and applications of polymerase chain reaction in medical diagnostic fields: a review
Provedor de dados: 58
Autores: Valones,Marcela Agne Alves; Guimarães,Rafael Lima; Brandão,Lucas André Cavalcanti; Souza,Paulo Roberto Eleutério de; Carvalho,Alessandra de Albuquerque Tavares; Crovela,Sergio.
Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conventional PCR; Real-Time PCR; Molecular Biology; Methods of molecular detection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100001