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A broad-range PCR technique for the diagnosis of infective endocarditis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Boujelben,Ines; Gdoura,Radhouane; Hammami,Adnane.
Abstract Infective endocarditis (IE) remains a severe and potentially fatal disease demanding sophisticated diagnostic strategies for detection of the causative microorganisms. The aim of the present study was to develop a broad-range 16S ribosomal RNA gene polymerase chain reaction in the routine diagnostic of IE for the early diagnosis of fatal disease. A broad-range PCR technique was selected and evaluated in terms of its efficiency in the diagnosis of endocarditis using 19 heart valves from patients undergoing cardiovascular surgeries at the Habib Bourguiba Hospital of Sfax, Tunisia, on the grounds of suspected IE. The results demonstrated the efficiency of this technique particularly in cases involving a limited number of bacteria since it helped to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broad-range PCR; Infective endocarditis; Conventional methods; Negative culture; Phenotypic investigations.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300534