Consulta: subjectFacets:"Critical parameters"
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Data/hora: 02/06/2024 05:07:00

A fast and simple assay to quantify bacterial leukotoxin activity
Provedor de dados: Electronic Journal of Biotechnology
Autores: Oppermann,Tobias; Schwarz,Stefan; Busse,Nadine; Czermak,Peter.
Background: Mannheimia haemolytica is the primary bacterial pathogen in causing bovine respiratory disease with tremendous annual losses in the cattle industry. The leukotoxin from M. haemolytica is the predominant virulence factor. Several leukotoxin activity assays are available but not standardized regarding sample preparation and cell line. Furthermore, these assays suffer from a high standard error, a prolonged time consumption and often complex sample pretreatments, which is important from the bioprocess engineering point of view. Results: Within this study, an activity assay based on the continuous cell line BL3.1 combined with a commercial available adenosine triphosphate viability assay kit was established. The leukotoxin activity was found to be...
Tipo: Journal article
Palavras-chave: ATP assay; Bacterial pathogen; Bovine respiratory disease; Cattle industry; Critical parameters; Lipopolysaccharides; Mannheimia haemolytica; Microbial biotechnology; Polymyxin B; Quantification; Virulence.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600006