Consulta: subjectFacets:"Cryoprotective agents"
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Data/hora: 09/06/2024 18:15:38

Evaluation of different cryoprotective agents in maintenance of viability of Helicobacter pylori in stock culture media
Provedor de dados: 58
Autores: Oskouei,Daryoush Davoudi; Bekmen,Neslihan; Ellidokuz,Hülya; Yilmaz,Özlem.
Four different cryoprotective supplemented stock media were evaluated for maintaining better survival and recovery of H. pylori type strain NCTC 11637 at two different maintenance temperatures of -20°C and -80°C after one month preservation as frozen stocks. The spread plate colony count method was used to investigate the recovery rate of H. pylori from equally inoculated bacterial suspensions in differently prepared stock cultures. After the preservation of H. pylori for one month in different cryoprotectant-supplemented stock media, the recovery rates for -20°C obtained for stock cultures supplemented with dimethyl sulfoxide (DMSO), polyethylene glycol (PEG), glycerol and glycerol+sucrose, as well as controls with and without human serum alone were 7.13,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Recovery rate; Stock culture media; Cryoprotective agents.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400023