Consulta: subjectFacets:"Cytochrome b"
Registros recuperados: 18
Data/hora: 17/06/2024 02:24:23

Molecular taxonomy of Plagioscion Heckel (Perciformes, Sciaenidae) and evidence from mtDNA RFLP markers for an invasive species in the Paraná river, Southern Brazil
Provedor de dados: 104
Autores: Torres,Rodrigo A.
Mitochondrial RFLP markers were developed to examine whether Plagioscion squamosissimus (Heckel, 1840) is invasive in natural environments of the congener P. ternetzi in the Paraná river, in southern Brazil. Specimens of P. squamosissimus and of the putative P. ternetzi (Boulenger, 1895) were obtained from the Negro river (Manaus, Amazonas, Brazil) and from Paraná river, respectively. Fragments of the cytochrome b gene (900bp) were amplified by PCR and four restriction enzymes (Eco RI, Mbo I, Bam HI and Alu I) yielded the mitochondrial markers. An additional RFLP analysis with a cytochrome b gene sequence of Plagioncion sp. from GeneBank was carried out to validate the prior analysis. No genetic differentiation was found among either sample. While...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plagioscion squamosissimus; Cytochrome b; Genetic identity; Biological invasions.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752006000400034

A NEW RECORD OF Leishmania (Viannia) guyanensis (Trypanosomatidae) FROM THE PACIFIC COAST OF COLOMBIA
Provedor de dados: 37
Autores: MARTINEZ ABAD,Lily Paola; PATERNINA TUIRÁN,Luís Enrique; PATERNINA-GÓMEZ,Margaret; PÉREZ DORIA,Alveiro; BEJARANO,Eduar Elias.
ABSTRACT The aim of this study was the identification of Leishmania species that causes cutaneous leishmaniasis in a patient from Buenaventura, Valle del Cauca, on the Pacific coast of Colombia. Clinical samples were obtained from a 29 years-old male who presented a distinct ulcer with raised borders on his neck. Samples were taken for direct microscopic examination, parasite culture, and molecular identification of the infecting Leishmania species by sequencing of the cytochrome b gene. Direct examination was positive for amastigotes of Leishmania but the culture was negative. The infecting parasite species was identified as L. (V.) guyanensis by means of the nucleotide sequence of a 509 bp fragment of the cytochrome b gene. We report the presence of L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniases; Cytochrome b; Epidemiology; Parasites.
Ano: 2021
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2021000100135

Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
Provedor de dados: 58
Autores: Shabbiri,Khadija; Ahmad,Waqar; Syed,Quratulain; Adnan,Ahmad.
A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteus mirabilis; Complex II; Cytochrome b.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300032

Identificación molecular de heces y análisis de hábitos alimenticios de carnívoros en la reserva de la biósfera "Sierra del Abra Tanchipa", San Luis Potosí, México.
Provedor de dados: 32
Autores: Benítez Alemán, Héctor Eduardo.
La identificación precisa de heces de carnívoros silvestres es  importante para describir sus hábitos alimenticios, sobre todo en aquellas especies elusivas o en riesgo. Sin embargo Identificar las heces de especies de carnívoros simpátricos no es una tarea sencilla. Por ello, las técnicas moleculares, son herramientas que brindan mayor precisión en la identificación de heces. El análisis de hábitos alimenticios aporta información valiosa para entender la estructura poblacional de los depredadores y sus presas, así como su importancia ecológica para generar estrategias de manejo y conservación de carnívoros y sus presas. Los objetivos de este estudio fueron identificar las heces de carnívoros extrayendo ADN mitocondrial de estas y amplificando un fragmento...
Palavras-chave: ADN mitocrondrial; Citocromo b; PCR-RFLP; Hábitos alimenticios; Carnívoros; Mitochondrial DNA; Cytochrome b; Food habits; Carnivores; Ganadería; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2291

Genetic differentiation of Macrodon ancylodon (Sciaenidae, Perciformes) populations in Atlantic coastal waters of South America as revealed by mtDNA analysis
Provedor de dados: 74
Autores: Santos,Simôni; Schneider,Horacio; Sampaio,Iracilda.
The king weakfish (pescada-gó in Portuguese - Macrodon ancylodon (Sciaenidae), a demersal (bottom-feeding) species found in South America Atlantic coastal waters from the Gulf of Paria in Venezuela to Baia Blanca in Argentina, is an economically important species because of its abundance and wide acceptance by consumers. Because of its wide distribution this fish may be subject to geographic isolation and this may have resulted in distinct populations along its coastal range. Considering that this species represents an important economic resource, confirmation of whether M. ancylodon is a single species or there are different genetic stocks spread over its wide distribution would be an important contribution to conservation policies and population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrodon ancylodon; Sciaenidae; Perciformes; 16S rRNA; Cytochrome b; Genetic differentiation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200008

Population genetic analysis of Caiman crocodilus (Linnaeus, 1758) from South America
Provedor de dados: 74
Autores: Vasconcelos,William R.; Hrbek,Tomas; Silveira,Ronis da; Thoisy,Benoit de; Marioni,Boris; Farias,Izeni P.
The genetic structure of Caiman crocodilus was investigated using a 1085 bp mtDNA fragment of the cytochrome b gene. Inferences were based on 125 individuals from nine localities in Peru, Brazil and French Guiana. With the exception of Mamirauá Lake, Anavilhanas Archipelago and the Tapará Community which show a signal of demographic expansion, the sampled localities are in a mutation-drift genetic equilibrium. Divergence between the Amazon basin and extra-Amazon basin localities is significant; however, inference from Nested Clade Analysis cannot distinguish between continuous range expansion, long distance colonization or past fragmentation; however, past fragmentation is unlikely due to low number of mutational steps separating these two regions. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic structure; Phylogeography; Genetic diversity; Demographic expansion; Cytochrome b; Caiman crocodilus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200006

Differentiation of Melipona quadrifasciata L. (Hymenoptera, Apidae, Meliponini) subspecies using cytochrome b PCR-RFLP patterns
Provedor de dados: 74
Autores: Souza,Rogério O.; Moretto,Geraldo; Arias,Maria C.; Del Lama,Marco A.
Melipona quadrifasciata quadrifasciata and M. quadrifasciata anthidioides are subspecies of M. quadrifasciata, a stingless bee species common in coastal Brazil. These subspecies are discriminated by the yellow stripe pattern of the abdominal tergites. We found Vsp I restriction patterns in the cytochrome b region closely associated to each subspecies in 155 M. quadrifasciata colonies of different geographical origin. This mitochondrial DNA molecular marker facilitates diagnosis of M. quadrifasciata subspecies matrilines and can be used to establish their natural distribution and identify hybrid colonies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subspecies differentiation; Melipona quadrifasciata; Cytochrome b; MtDNA; PCR-RFLP patterns.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300009

Thaptomys Thomas 1915 (Rodentia, Sigmodontinae, Akodontini) with karyotypes 2n = 50, FN = 48, and 2n = 52, FN = 52: two monophyletic lineages recovered by molecular phylogeny
Provedor de dados: 74
Autores: Ventura,Karen; Silva,Maria José de Jesus; Yonenaga-Yassuda,Yatiyo.
A novel karyotype with 2n = 50, FN = 48, was described for specimens of Thaptomys collected at Una, State of Bahia, Brazil, which are morphologically indistinguishable from Thaptomys nigrita, 2n = 52, FN = 52, found in other localities. It was hence proposed that the 2n = 50 karyotype could belong to a distinct species, cryptic of Thaptomys nigrita, once chromosomal rearrangements observed, along with the geographic distance, might represent a reproductive barrier between both forms. Phylogenetic analyses using maximum parsimony and maximum likelihood based on partial cytochrome b sequences with 1077 bp were performed, attempting to establish the relationships among the individuals with distinct karyotypes along the geographic distribution of the genus;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic Rainforest; Cytochrome b; Endemism; Molecular phylogeny; Thaptomys.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200010

Genetic evidence for a species complex within the piranha Serrasalmus maculatus (Characiformes, Serrasalmidae) from three Neotropical river basins based on mitochondrial DNA sequences
Provedor de dados: 74
Autores: Bignotto,Thaís Souto; Maniglia,Thiago Cintra; Gomes,Vivian Nunes; Oliveira,Isadora Janolio de; Agostinho,Carlos Sérgio; Prioli,Sônia Maria Alves Pinto; Prioli,Alberto José.
Abstract Mitochondrial molecular markers (DNA sequences of D-loop, cytochrome b and cytochrome c oxidase I) were employed to characterize populations of the piranha Serrasalmus maculatus from Upper Paraná, Upper Paraguay and Tocantins River basins. D-loop sequences of S. maculatus population from Paraná-Paraguay River basin exhibited tandem repeats of short motifs (12 base pairs) and variable numbers depending on specimens, accounting for length variation. Concatenated mitochondrial sequences suggested that S. maculatus encompasses different mitochondrial DNA lineages. Although sampling was restricted to three river basins, phylogenetic analysis clearly indicated that the species currently recognized as S. maculatus presents high genetic variability....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptic species; Species delimitation; D-loop; Cytochrome b; Cytochrome c oxidase I.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100801

Testing the Rio Doce as a riverine barrier in shaping the Atlantic rainforest population divergence in the rodent Akodon cursor
Provedor de dados: 74
Autores: Colombi,Victor Hugo; Lopes,Silvia Ramira; Fagundes,Valéria.
Akodon cursor occurs in dense rainforest from northern (8º S) to southern (26º S) states along the Atlantic coast of Brazil. Previous karyological and molecular data revealed two major clades, one including northern (8-15º S) and the other southern (19-26º S) populations. The center of geographic distribution (15-20º S), which included the state of Espírito Santo, was identified as a potential vicariance region. Since river barriers are among the most discussed models in the study of Neotropical diversification, we examined whether the Rio Doce (19º S) plays an important role in shaping the population genetic divergence of A. cursor by including samples from Espírito Santo in the analysis. Our results showed that the northern-southern division region in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Akodontini; Atlantic Forest; Cytochrome b; Gene flow; Phylogeography; Rodentia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400029

Molecular identification of Mazama species (Cervidae: Artiodactyla) from natural history collections
Provedor de dados: 74
Autores: Mantellatto,Aline Meira Bonfim; González,Susana; Duarte,José Maurício Barbanti.
Abstract Natural history museum collections constitute an invaluable patrimony of biological diversity for analysing the taxa distribution and evolution. However, it is very common to discover taxonomic misidentification in museum collections based on incorrect data. The aim of this research was to identify brocket deer species (Mazama genus) using molecular markers. We collected 199 samples, performed DNA extraction and species identification using a specific mitochondrial marker based on a fragment of cytochrome b (Cytb) for Neotropical deer. We achieved the amplification and sequencing of 77 specimens and verified that 26% of the skulls were wrongly identified. Moreover, in the museum collections 57% of the specimens were only identified as Mazama sp,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome b; Deer; DNA; Mammal; Morphological taxonomy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400201

Diversification of Ramphastinae (Aves, Ramphastidae) prior to the Cretaceous/Tertiary boundary as shown by molecular clock of mtDNA sequences
Provedor de dados: 74
Autores: Nahum,Laila Alves; Pereira,Sérgio Luiz; Campos Fernandes,Flora Maria de; Russo Matioli,Sergio; Wajntal,Anita.
Partial cytochrome b and 12S rDNA mitochondrial DNA sequences of eight representatives of the Ramphastidae family were analyzed. We applied the linearized tree method to identify sequences evolving at similar rates and estimated the divergence times among some of the taxa analyzed. After excluding Ramphastos tucanus and Capito dayi from our data set, the remaining taxa presented a constant rate of DNA substitution, and branch lengths could be re-estimated with a clock constraint using the maximum likelihood method. Branch lengths were calibrated assuming that Galliformes and Piciformes split around 100 million years ago (mya). Our results indicate that Ramphastinae, and probably Capitoninae, diverged from other Piciformes in the Late Cretaceous (~82 mya),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Divergence times; K/T; Mass extinction; Cytochrome b; 12S rDNA; Paleogeography; Phylogeny.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400003

Phylogeny of Thylamys (Didelphimorphia, Didelphidae) species, with special reference to Thylamys karimii
Provedor de dados: 76
Autores: Carvalho,Bianca de A; Oliveira,Luiz F. B; Mattevi,Margarete S.
The genus Thylamys Gray, 1843 lives in the central and southern portions of South America inhabiting open and shrub-like vegetation, from prairies to dry forest habitats in contrast to the preference of other Didelphidae genera for more mesic environments. Thylamys is a speciose genus including T. elegans (Waterhouse, 1839), T. macrurus (Olfers, 1818), T. pallidior (Thomas, 1902), T. pusillus (Desmarest, 1804), T. venustus (Thomas, 1902), T. sponsorius (Thomas, 1921), T. cinderella (Thomas, 1902), T. tatei (Handley, 1957), T. karimii (Petter, 1968), and T. velutinus (Wagner, 1842) species. Previous phylogenetic analyses in this genus did not include the Brazilian species T. karimii, which is widely distributed in this country. In this study, phylogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome b; Didelphidae; Didelphimorphia; South America; Thylamys phylogeny.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212009000400012

Genetic structure of the reef grouper Epinephelus merra in the West Indian Ocean appears congruent with biogeographic and oceanographic boundaries
Provedor de dados: 5
Autores: Muths, Delphine; Tessier, Emmanuel; Bourjea, Jerome.
The reef fauna connectivity of the West Indian Ocean (WIO) is one of the least studied globally. Here we use genetic analyses of the grouper Epinephelus merra (Bloch 1793) to determine patterns of connectivity and to identify barriers to dispersal in this WIO marine area. Phylogeographic and population-level analyses were conducted on cytochrome b sequences and microsatellites (13 loci) from 557 individuals sampled in 15 localities distributed across the West Indian Ocean. Additional samples from the Pacific Ocean were used to benchmark the WIO population structure. The high level of divergence revealed between Indian and Pacific localities (of about 4.5% in sequences) might be the signature of the major tectonic and climatic changes operating at the...
Tipo: Text
Palavras-chave: Cytochrome b; Marine connectivity; Microsatellite; Reef fish; West Indian Ocean.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00189/30046/28566.pdf

Genetic structure and demographic history of striped weakfish Cynoscion guatucupa (sciaenidae) from the southwestern atlantic
Provedor de dados: 46
Autores: Alonso,M.P; Fernández Iriarte,P.J.
In South America, the Pleistocene was characterized by important environmental changes related to glacial cycles, which had significant effects on the evolutionary history of several marine species. To evaluate the pattern of demographic history and the genetic structure of striped weakfish Cynoscion guatucupa, a 401 bp fragment of the mitochondrial Cytochrome b gene was sequenced from 92 individuals from three coastal areas in Argentina and one in Brazil in the southwestern Atlantic. Haplotype diversity was high, whereas nucleotide diversity was low among all sampling sites. The star-like pattern was not phylogeographically structured, in agreement with the AMOVA analysis. The Fu&amp;#8217;s test was negative and highly significant whereas the mismatch...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic structure; Cytochrome b; Marine fish; Pleistocene; Mutation rate.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332015000300001

Low genetic variability in the endangered Colombian endemic freshwater turtle Podocnemis lewyana (Testudines, Podocnemididae)
Provedor de dados: 16
Autores: Vargas-Ramírez, M.; Chiari, Y.; Castaño-Mora, O.V.; Menken, S.B.J.
The Magdalena River Turtle (Podocnemis lewyana) is a Colombian endemic species, endangered due to human exploitation and habitat destruction. To date, this species is poorly known ecologically and data on its genetic diversity are lacking. Here we report on the first genetic survey of the species across its distribution range. We obtained mitochondrial DNA sequences (488 bp) of the cytochrome b gene from 109 individuals. Samples belong to populations located at several different localities, grouped in five regions, along the four main river basins: Magdalena, Cauca, San Jorge, and Sinú drainages. We found two haplotypes, which differ in only one nucleotide substitution and which are represented with different frequencies in the five geographic regions....
Tipo: Article / Letter to the editor
Palavras-chave: Reptilia; Genetic population structure; Cytochrome b; MtDNA; Podocnemis; 42.82.
Ano: 2007
URL: http://www.repository.naturalis.nl/record/217421

Preliminary study on phylogeography of Callosciurus prevostii in Southeast Asia : mitochondrial DNA evidence supports riverine barrier hypothesis
Provedor de dados: 19
Autores: Oshida, Tatsuo; Yasuda, Masatoshi; Sasaki, Motoki.
Evolutionary history of Callosciurus squirrel species seems affected by geographical-isolation by huge rivers in Southeast Asia. Geographical isolation by rivers may have influenced phylogeographical structure between and within Callosciurus species. In Sundaland, Southeast Asia, however, phylogeographical structure within Callosciurus species may be explained by geographical isolation among islands. In a test of the effects of rivers and islands, we examined phylogeographical characteristics of C. prevostii based on mitochondrial cytochrome b gene sequences. This squirrel is distributed from peninsular Thailand to Sumatra and Borneo and adjacent small islands. We collected specimens from four geographically different populations (the Malay Peninsula,...
Palavras-chave: Cytochrome b; Geographical isolation; Prevost's squirrel; Sumatra Island.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4470

Molecular diversity among domestic guinea-pigs (Cavia porcellus) and their close phylogenetic relationship with the Andean wild species Cavia tschudii
Provedor de dados: 97
Autores: SPOTORNO,ÁNGEL E; VALLADARES,JOHN P; MARÍN,JUAN C; ZEBALLOS,HORACIO.
To investigate the origin and diversity of domestic guinea-pigs Cavia porcellus (Linnaeus, 1758; Rodentia, Caviidae), we sequenced the mitochondrial cytochrome b gene of 12 domestic and 10 wild specimens from six species, including the two presumed as ancestral to the domestic one: Cavia tschudii and Cavia aperea. All maximum parsimony and maximum likelihood analyses grouped C. porcellus with C. tschudii (mean K2P distance = 3.2 %); best trees had 609 steps (CI = 0.796; Bremer support Index (SI) = 28), and a _Ln = 4419.52, with 100 % and 97 % bootstrap support respectively. This clade, supported by three substitutions and 96 % bootstrap, is also obtained in the cladistic analysis of corresponding amino acids. When the C. aperea node was forced to join C....
Tipo: Journal article
Palavras-chave: Andes; Cavidae; Cytochrome b; Domestication; Guinea pig; Molecular phylogeny.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2004000200004