Consulta: subjectFacets:"Cytoskeleton"
Registros recuperados: 10
Data/hora: 08/06/2024 09:36:55

Morphology and histochemistry of glandular trichomes in Hyptis villosa Pohl ex Benth. (Lamiaceae) and differential labeling of cytoskeletal elements
Provedor de dados: 39
Autores: Tozin,Luiz Ricardo dos Santos; Rodrigues,Tatiane Maria.
ABSTRACT Lamiaceae contains many species known for their aromatic properties that are produced by the production of essential oils in glandular trichomes. Hyptis is one of the most common genera of Lamiaceae in the Brazilian flora, and includes several species with medicinal value. However, studies on the morphology and functioning of their glandular trichomes are lacking. We analyzed the morphology, histochemistry and ultrastructure of the glandular trichomes in leaves of H. villosa, emphasizing the differential distribution of actin filaments and microtubules in cells secreting hydrophilic and lipophilic compounds. Four morphotypes of glandular trichomes were identified. Total lipid, terpenes, alkaloids, phenolic compounds, proteins and polysaccharides...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actin microfilaments; Cytoskeleton; Glandular hairs; Microtubules; Ultrastructure.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300330

The cytoskeleton of the electric tissue of Electrophorus electricus, L.
Provedor de dados: 42
Autores: MERMELSTEIN,CLAUDIA DOS SANTOS; COSTA,MANOEL LUIS; MOURA NETO,VIVALDO.
The electric eel Electrophorus electricus is a fresh water teleost showing an electrogenic tissue that produces electric discharges. This electrogenic tissue is distributed in three well-defined electric organs which may be found symmetrically along both sides of the eel. These electric organs develop from muscle and exhibit several biochemical properties and morphological features of the muscle sarcolema. This review examines the contribution of the cytoskeletal meshwork to the maintenance of the polarized organization of the electrocyte, the cell that contains all electric properties of each electric organ. The cytoskeletal filaments display an important role in the establishment and maintenance of the highly specialized membrane model system of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrophorus electricus; Cytoskeleton; Desmin; Actin; Alpha-actinin; Vinculin.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300008

The in vitro comparative cytopathology of a porcine rotavirus and the simian prototype (SA-11)
Provedor de dados: 45
Autores: Lonien,S.C.H.; Bolognini,A.M.; Linhares,R.E.C.; Nozawa,C.M.
A citopatologia in vitro de uma cepa de rotavírus porcino adaptado em cultura de células foi comparada à estirpe-protótipo símia (SA-11). O efeito citopático (ECP) produzido pelos vírus foi semelhante embora a estirpe porcina tivesse apresentado algumas alterações diferentes, como o acentuado estreitamento do citoplasma, com grande perda do volume citoplasmático. O vírus porcino apresentou menor número de plaques de ECP porém com diâmetro maior em relação ao vírus símio, demonstrando maior capacidade de disseminação célula-célula, quase oito vezes mais, a julgar pelo diâmetro dos plaques de ECP. Os elementos do citoesqueleto das células infectadas revelaram uma reorganização semelhante para ambas as estirpes, não sendo possível observar nenhuma diferença,...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Rotavirus; Cytopathology; Cell culture; Plaque; Cytoskeleton.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000400002

A simple and effective pressure culture system modified from a transwell cell culture system
Provedor de dados: 48
Autores: Sun,Xuerong; Liu,Xinguang; Zhang,Yuehong; Kuang,Xielan; Lv,Bo; Ge,Jian.
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin &amp;#946;1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is...
Tipo: Journal article
Palavras-chave: Mechanical pressure; Cell culture; Transwell culture system; Cytoskeleton.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007

Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells
Provedor de dados: 48
Autores: Xu,Huiyun; Zhang,Jian; Wu,Jiawei; Guan,Ying; Weng,Yuanyuan; Shang,Peng.
Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. However, there is little information about the response of integrin-associated molecules using both osteoblasts and osteocytes. In this study, we investigated the changes in response to 2 hours of oscillatory fluid flow stress in the MLO-Y4 osteocyte-like cell line and the MC3T3-E1 osteoblast-like cell line. MLO-Y4 cells exhibited a significant increase in the expression of integrin-associated molecules, including OPN, CD44, vinculin and integrin avp3. However,...
Tipo: Journal article
Palavras-chave: Cytoskeleton; Fluid flow stress; Integrin-associated molecules; MC3T3-E1 osteoblast-like cell line; MLO-Y4 osteocyte-like cell line.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200008

Dexamethasone blocks the migration of the human neuroblastoma cell line SK-N-SH
Provedor de dados: 56
Autores: Casulari,L.A.; Dondi,D.; Maggi,R.; Demissie,M.; Piccolella,M.; Piva,F.
Glucocorticoids (Gc) influence the differentiation of neural crest-derived cells such as those composing sympathoadrenal tumors like pheochromocytomas, as well as neuroblastomas and gangliomas. In order to obtain further information on the effects of Gc on cells evolving from the neural crest, we have used the human neuroblastoma cell line SK-N-SH to analyze: 1) the presence and the binding characteristics of Gc receptors in these cells, 2) the effect of dexamethasone (Dex) on the migration of SK-N-SH cells, and 3) the effect of Dex on the organization of the cytoskeleton of SK-N-SH cells. We show that: 1) receptors that bind [³H]-Dex with high affinity and high capacity (Kd of 9.6 nM, Bmax of 47 fmol/mg cytosolic protein, corresponding to 28,303...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human neuroblastoma; SK-N-SH cells; Glucocorticoids; Migration; Cytoskeleton.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900011

Hypothyroidism decreases proinsulin gene expression and the attachment of its mRNA and eEF1A protein to the actin cytoskeleton of INS-1E cells
Provedor de dados: 56
Autores: Goulart-Silva,F.; Serrano-Nascimento,C.; Nunes,M.T.
The actions of thyroid hormone (TH) on pancreatic beta cells have not been thoroughly explored, with current knowledge being limited to the modulation of insulin secretion in response to glucose, and beta cell viability by regulation of pro-mitotic and pro-apoptotic factors. Therefore, the effects of TH on proinsulin gene expression are not known. This led us to measure: a) proinsulin mRNA expression, b) proinsulin transcripts and eEF1A protein binding to the actin cytoskeleton, c) actin cytoskeleton arrangement, and d) proinsulin mRNA poly(A) tail length modulation in INS-1E cells cultured in different media containing: i) normal fetal bovine serum - FBS (control); ii) normal FBS plus 1 µM or 10 nM T3, for 12 h, and iii) FBS depleted of TH for 24 h (Tx)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proinsulin mRNA; EEF1A protein; Cytoskeleton; Hypothyroidism; INS-1E cells.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000012

Cytoskeletal and cellular adhesion proteins in zebrafish (Danio rerio) myogenesis
Provedor de dados: 56
Autores: Costa,M.L.; Escaleira,R.; Manasfi,M.; Souza,L.F. de; Mermelstein,C.S.
The current myogenesis and myofibrillogenesis model has been based mostly on in vitro cell culture studies, and, to a lesser extent, on in situ studies in avian and mammalian embryos. While the more isolated artificial conditions of cells in culture permitted careful structural analysis, the actual in situ cellular structures have not been described in detail because the embryos are more difficult to section and manipulate. To overcome these difficulties, we used the optically clear and easy to handle embryos of the zebrafish Danio rerio. We monitored the expression of cytoskeletal and cell-adhesion proteins (actin, myosin, desmin, alpha-actinin, troponin, titin, vimentin and vinculin) using immunofluorescence microscopy and video-enhanced,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytoskeleton; Cell adhesion; Zebrafish; Myogenesis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800019

Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation
Provedor de dados: 56
Autores: Mermelstein,C.S.; Rebello,M.I.L.; Amaral,L.M.; Costa,M.L.
Although much is known about the molecules involved in extracellular Ca2+ regulation, the relationship of the ion with overall cell morphology is not understood. The objective of the present study was to determine the effect of the Ca2+ chelator EGTA on the major cytoskeleton components, at integrin-containing adhesion sites, and their consequences on cell shape. Control mouse cell line C2C12 has a well-spread morphology with long stress fibers running in many different directions, as detected by fluorescence microscopy using rhodamine-phalloidin. In contrast, cells treated with EGTA (1.75 mM in culture medium) for 24 h became bipolar and showed less stress fibers running in one major direction. The adhesion plaque protein alpha5-integrin was detected by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytoskeleton; Extracellular Ca2+; Cell shape; Microfilaments; Microtubules; Integrin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800018

Recent advances in aluminum toxicity and resistance in higher plants
Provedor de dados: 61
Autores: Vitorello,Victor Alexandre; Capaldi,Flávia Regina; Stefanuto,Vanderlei Antonio.
Aluminum toxicity is a major soil constraint to food and biomass production throughout the world. Considerable advances in the understanding of the mechanism of resistance involving exudation of organic acids have been made in recent years. However, despite intense research efforts, there are many aspects of Al toxicity that remain unclear. This article reviews the features of the chemistry of Al relevant to its toxicity followed by an examination of the mechanisms of toxicity and resistance. Emphasis, however, is given to the mechanisms of Al toxicity, since resistance has been covered recently by several reviews. Some topics which are specifically discussed in this review are: a) The possible role of cellular effects of low pH in Al toxicity, which has...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Al uptake and localization; Cell wall; Cytoskeleton; Differential sensitivity to Al; Low pH; Acidity; Plasma membrane.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100011