Consulta: subjectFacets:"Cytotoxicity"
Registros recuperados: 116
Data/hora: 04/06/2024 04:11:09

Estudio etnofarmacológico de especies vegetales con actividad hipoglucemiante en la comunidad de Malpasito en Huimanguillo, Tabasco.
Provedor de dados: 32
Autores: Villarreal Ibarra, Edelia Claudina.
Ante el incremento de enfermedades crónico-degenerativas uno de los recursos terapéuticos por excelencia lo representa la medicina tradicional, la presente investigación documenta y evalúa farmacológicamente las plantas utilizadas para el control de la Diabetes Mellitus (DM) en la comunidad de Malpasito, dentro de la Reserva Ecológica de Agua Selva en Huimanguillo, Tabasco, México como un aporte a la validación científica. Registra un inventario del conocimiento tradicional de las plantas medicinales, con información sobre el uso terapéutico, forma de preparación y parte vegetal utilizada. La información se recabó mediante entrevistas estructuradas TRAMIL. Se registraron 128 especies distribuidas en 63 familias botánicas, los usos medicinales se agruparon...
Palavras-chave: Medicina tradicional; Diabetes; Citotoxicidad; Artemia salina; Traditional medicine; Cytotoxicity; Ciencias por Investigación; Doctorado.
Ano: 2014
URL: http://hdl.handle.net/10521/2278

Evaluation of antimicrobial activity and cell viability of Aloe vera sponges
Provedor de dados: 69
Autores: Gontijo,Sávio Morato de Lacerda; Gomes,Alinne Damásia Martins; Gala-García,Alfonso; Sinisterra,Rubén Dario; Cortés,Maria Esperanza.
Background: Aloe vera L., member of the Liliaceae family, has been shown to stimulate cell proliferation and contribute to healing and angiogenesis, has anti-bacterial, anti-fungal and anti-inflammatory activity. In addition, Aloe vera can be used as a support for drug transport. Our objective is to evaluate antimicrobial activity and cytotoxicity of sponges of Aloe vera L. for use as a carrying support of drugs. Results: In this work, sponge of free Aloe vera (AV) loaded with amoxicillin (AMX) or nystatin (NYS) at 1% w/w, were prepared and physico-chemically characterized via X-ray diffraction, Fourier Transform Infrared Spectroscopy and thermal analysis. Antimicrobial potency of AV sponge alone, loaded with AMX or NYS, against strains of Streptococcus...
Tipo: Journal article
Palavras-chave: Amoxicillin; Cytotoxicity; Drug transport; Nystatin.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100002

Assessment of the genetic risks of a metallic alloy used in medical implants
Provedor de dados: 74
Autores: Gomes,Cristiano C.; Moreira,Leonardo M.; Santos,Vanessa J.S.V.; Ramos,Alfeu S.; Lyon,Juliana P.; Soares,Cristina P.; Santos,Fabio V.
The use of artificial implants provides a palliative or permanent solution for individuals who have lost some bodily function through disease, an accident or natural wear. This functional loss can be compensated for by the use of medical devices produced from special biomaterials. Titanium alloy (Ti-6Al-4V) is a well-established primary metallic biomaterial for orthopedic implants, but the toxicity of the chemical components of this alloy has become an issue of concern. In this work, we used the MTT assay and micronucleus assay to examine the cytotoxicity and genotoxicity, respectively, of an extract obtained from this alloy. The MTT assay indicated that the mitochondrial activity and cell viability of CHO-K1 cells were unaffected by exposure to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomaterial; Cytotoxicity; Micronucleus; Mutagenicity; Ti-6Al-4V.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100020

Profile of micronucleus frequencies and DNA damage in different species   of fish in a eutrophic tropical lake
Provedor de dados: 74
Autores: Grisolia,Cesar K.; Rivero,Carla L. G.; Starling,Fernando L. R. M.; Silva,Izabel C. R. da; Barbosa,Antonio C.; Dorea,Jose G.
Lake Paranoá is a tropical reservoir for the City of Brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. At present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. We evaluated both genotoxic and cytotoxic parameters in several fish species ( Geophagus brasiliensis ,  Cichla temensis ,  Hoplias malabaricus ,  Astyanax bimaculatus lacustres ,  Oreochromis niloticus ,  Cyprinus carpio  and  Steindachnerina insculpita ) by using the micronucleus (MN) test, the comet assay and nuclear abnormality assessment in peripheral erythrocytes. The highest frequencies of MN were found in  Cichla temensis  and  Hoplias...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Fish biomonitoring; Eutrophic lake; Micronucleus; Comet assay; Cytotoxicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100021

Studies of micronuclei and other nuclear abnormalities in red blood cells of Colossoma macropomum exposed to methylmercury
Provedor de dados: 74
Autores: Rocha,Carlos Alberto Machado da; Cunha,Lorena Araújo da; Pinheiro,Raul Henrique da Silva; Bahia,Marcelo de Oliveira; Burbano,Rommel Mario Rodríguez.
The frequencies of micronuclei (MN) and morphological nuclear abnormalities (NA) in erythrocytes in the peripheral blood of tambaqui (Colossoma macropomum), treated with 2 mg.L-1 methylmercury (MeHg), were analyzed. Two groups (nine specimens in each) were exposed to MeHg for different periods (group A - 24 h; group B - 120 h). A third group served as negative control (group C, untreated; n = 9). Although, when compared to the control group there were no significant differences in MN frequency in the treated groups, for NA, the differences between the frequencies of group B (treated for 120 h) and the control group were extremely significant (p &lt; 0.02), thus demonstrating the potentially adverse effects of MeHg on C. macropomum erythrocytes after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Genotoxicity; Methylmercury; Micronuclei assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400024

LNO3 AND L3 Are Associated With Antiproliferative And Pro-Apoptotic Action In Hepatoma Cells
Provedor de dados: 74
Autores: Zanelatto,Leonardo Campos; Silva,Patrícia Benites Gonçalves da; Sartori,Daniele; Panis,Carolina; Lepri,Sandra; Fátima,Ângelo de; Mantovani,Mário Sérgio.
Abstract The identification of antitumoral substances is the focus of intense biomedical research. Two structural analogues of thalidomide, LNO3 and L3, are two synthetic compounds that might possess such antitumor properties. We evaluated the toxicological effects of these substances, including cytotoxicity, genotoxicity and induction of apoptosis in HTC cells. Additionally, the production of free radicals (nitric oxide and superoxide) was investigated, and the expression of caspases genes 3, 8, and 9 were determined by RT-qPCR. The compounds exhibited cytotoxic effects that resulted in inhibited cell proliferation. LNO3 showed to be more effective and toxic than L3 in all assays. LNO3 stimulated the release of NO and superoxide, which was accompanied by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thalidomide analogues; Antiproliferative activity; Cytotoxicity; Free radicals; Apoptosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200270

Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[a]pyrene and cyclophosphamide
Provedor de dados: 74
Autores: Araujo,Caroline de S.; Brito,Lorrane D.; Tarifa,Marina O.; Silva,Nayara J. Farah da; Rodrigues,Karoline S.; Cavalcante,Dalita G. S. M.; Gomes,Andressa S.; Zocoler,Marcos A.; Yoshihara,Eidi; Camparoto,Marjori L.; Job,Aldo E.; Kerche,Leandra E.
Abstract This study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a]pyrene (B[a]P) and cyclophosphamide (CP) were chosen as DNA damage inducers. Genotoxicity and antigenotoxicity were evaluated by the comet assay. Cytotoxicity, mutagenicity, and antimutagenicity were evaluated by the micronucleus test in bone marrow and peripheral blood. The biochemical parameters of oxidative stress were evaluated by the quantification of catalase activity (CAT) and reduced glutathione (GSH)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus test; Spondias dulcis; Cytotoxicity; Mutagenicity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400643

Mitodepressive and clastogenic effects of aqueous extracts of the lichens Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) on meristematic cells in plant bioassays
Provedor de dados: 74
Autores: Campos,José Marcello Salabert de; Davide,Lisete Chamma; Soares,Geraldo Luiz Gonçalves; Viccini,Lyderson Facio.
The cytotoxicity effect of aqueous extracts of the lichens species Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) were evaluated using meristematic cells of lettuce (Lactuca sativa) and maize (Zea mays). The seasonal effect was also evaluated. Extracts of M. lindmanii and C. texana inhibited root growth and/or percentage germination, possibly due to alterations in the cell cycle. The M. lindmanii extract showed anti-mitotic effects and blocked the cell cycle in metaphase so that c-mitosis and cells with chromosome duplication were produced. The C. texana extract appeared to hinder cell division, increasing the number of interphase cells. In addition, both extracts caused an increase in percentage of cell death. Clastogenic effects...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Canoparmelia texana; Cytotoxicity; Mitotic index; Myelochroa lindmanii; Plant bioassays.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100024

Cytotoxic and genotoxic potential of surface water from the Pitimbu river, northeastern/RN Brazil
Provedor de dados: 74
Autores: Egito,Lucila Carmem Monte; Medeiros,Maria das Graças; Medeiros,Silvia Regina Batistuzzo de; Agnez-Lima,Lucymara Fassarella.
In this study, the onion (Allium cepa) root test was used to evaluate the genotoxicity of the Pitimbu River (Natal city, Brazil) surface water. The water was collected at five sampling sites along the river and one sample was obtained after the treatment (flocculation, chlorination and pH correction) of the river water for human consumption. All raw river water samples increased the frequency of chromosomal abnormalities and/or micronuclei and two of the water samples produced alterations in the mitotic index of the root cells. Two of the water samples also altered root growth and two produced morphological modifications in the A. cepa roots. Water collected from a site near an industrial area was the most consistently toxic and genotoxic of the samples....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Micronucleus; Cytotoxicity; Genotoxicity; Surface water.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300023

Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays
Provedor de dados: 74
Autores: Dourado,Priscila Leocádia Rosa; Rocha,Monyque Palagano da; Roveda,Liriana Mara; Raposo Junior,Jorge Luiz; Cândido,Liliam Sílvia; Cardoso,Claudia Andréa Lima; Morales,Maria Aparecida Marin; Oliveira,Kelly Mari Pires de; Grisolia,Alexeia Barufatti.
Abstract This study aimed to evaluate DNA damage in animal and plant cells exposed to water from the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil) by using bioassays, and to identify the chemical compounds in the water to determine the water quality in the area. Through the cytotoxicity bioassay with Allium cepa, using micronucleus test, and comet assay, using Astyanax altiparanae fish, the results indicated that biological samples were genetically altered. Micronuclei were observed in erythrocytes of A. altiparanae after exposure to water from locations close to industrial waste discharge. The highest DNA damage observed with the comet assay in fish occurred with the exposure to water from locations where the presence of metals (Cu, Pb, Cd, Ni)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Astyanax altiparanae; Mutagenicity; Cytotoxicity; Genotoxicity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100123

Cytotoxicity and genotoxicity of stilbene derivatives in CHO-K1 and HepG2 cell lines
Provedor de dados: 74
Autores: Mizuno,Cassia Suemi; Ampomaah,Winnifred; Mendonça,Fernanda Ribeiro; Andrade,Gabriela Carvalho; Silva,Ariel Maria Nazaré da; Goulart,Mirian Oliveira; Santos,Raquel Alves dos.
Abstract The cytotoxicity and genotoxicity of the stilbenes (E)-methyl-4-(3-5-dimethoxystyryl)benzoate (ester), (E)-4-(3-5-dimethoxystyryl)aniline (amino), (Z)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (cis-TMS) and (E)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (trans-TMS) were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses. The parameters were evaluated using XTT assay, clonogenic survival assay and the cytokinesis-block micronucleus assay in CHO-K1 and HepG2 cell lines. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stilbene; Cytotoxicity; Genotoxicity; Micronucleus assay.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400656

Évaluation d’une suite de bio-essais pour la détection et l’étude de composés lipophiles de micro-organismes marins issus de mollusques bivalves et de leur environnement
Provedor de dados: 5
Autores: Geiger, Marie.
The context of this study was the assessment of the safety of edible bivalve molluscs. Bivalve can indeed accumulate high concentrations of phycotoxins into their hepatopancreas, potentially leading to human intoxication outbreaks after consumption of such contaminated bivalves. In order to ensure the protection of shellfish consumers, regulated toxins are monitored using physico-chemical techniques. In parallel, emerging toxins, that can be putatively produced by microalgae or marine fungi, are monitored using the intraperitoneal mouse bioassay. However, this bioassay suffers ethical and methodological drawbacks. Thus, the objective of this work was to assess the ability of a bioassay suite to detect lipophilic toxins. This suite was composed of three...
Tipo: Text
Palavras-chave: Cytotoxicité; Larves de diptères; Activité antibactérienne; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii; Cytotoxicity; Diptera larvae; Antibacterial activity; Lipophilic toxins; Microalgae; Dinoflagellates; Marine fungi; Penicillium; Okadaic acid; Azaspiracid; Pinnatoxin G; Vulcanodinium rugosum; Azadinium spinosum; Prorocentrum lima; Beauveria brongniartii.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00183/29426/27787.pdf

Cytotoxic and antibacterial properties of Mytilus galloprovincialis, Ostrea edulis and Crassostrea gigas (bivalve molluscs) hemolymph
Provedor de dados: 5
Autores: Hubert, Florence; Van Der Knaap, Wil; Noel, Thierry; Roch, Philippe.
Mussel (Mytilus galloprovincialis) plasma contains cytotoxic activity against both vertebrate (erythrocytes and mouse tumour) and protozoan cells. Procaryotes (Escherichia coli and Vibrio alginolyticus) were not sensitive to the cytotoxicity. The activity was still present in dialyzed samples but was inhibited by heating at 45°C. Large individual variability which was not correlated with protein concentration and an increasing number of reactive specimens following injection, suggested that naive mussels were in various stages of immune response. Purification by anion exchange chromatography followed by gel filtration revealed a 320 kDa cytotoxic polymeric protein that acts through a polymerization process after binding onto target cell membranes as...
Tipo: Text
Palavras-chave: Cytotoxicité; Antibactérien; Hémolymphe; Lysat d'hémocyte; Moule; Huître; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas; Cytotoxicity; Antibacterial; Hemolymph; Hemocyte lysate; Musssel; Oyster; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00039/15008/12333.pdf

A rapid quantitative fluorescence-based bioassay to study allelochemical interactions from Alexandrium minutum
Provedor de dados: 5
Autores: Long, Marc; Tallec, Kevin; Soudant, Philippe; Lambert, Christophe; Le Grand, Fabienne; Sarthou, Geraldine; Jolley, Dianne; Hegaret, Helene.
Harmful microalgal blooms are a threat to aquatic organisms, ecosystems and human health. Toxic dinoflagellates of the genus Alexandrium are known to produce paralytic shellfish toxins and to release bioactive extracellular compounds (BECs) with potent cytotoxic, hemolytic, ichtyotoxic and allelopathic activity. Negative allelochemical interactions refer to the chemicals that are released by the genus Alexandrium and that induce adverse effects on the physiology of co-occurring protists and predators. Releasing BECs gives the donor a competitive advantage that may help to form dense toxic blooms of phytoplankton. However BECs released by Alexandrium minutum are uncharacterized and it is impossible to quantify them using classical chemical methods....
Tipo: Text
Palavras-chave: Harmful algal bloom; Alexandrium; Allelopathy; Cytotoxicity; PAM fluorometry; Bioassay.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00453/56424/58116.pdf

Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers
Provedor de dados: 5
Autores: Geiger, Marie; Guitton, Yann; Vansteelandt, M.; Kerzaon, I.; Blanchet, Estelle; Du Pont, T. Robiou; Frisvad, J. C.; Hess, Philipp; Pouchus, Y. F.; Grovel, Olivier.
In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with...
Tipo: Text
Palavras-chave: Cytotoxicity; Dereplication; Marine fungi; Mass spectrometry; Penicillium; Shellfish.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00160/27166/25523.pdf

Polystyrene microbeads modulate the energy metabolism of the marine diatom Chaetoceros neogracile
Provedor de dados: 5
Autores: Seoane, Marta; González-fernández, Carmen; Soudant, Philippe; Huvet, Arnaud; Esperanza, Marta; Cid, Ángeles; Paul-pont, Ika.
Due to the growing concern about the presence of microplastics (MP) in the environment, the number of studies evaluating the toxicity of these small persistent particles on different marine species has increased in recent years. Few studies have addressed their impact on marine phytoplankton, a subject of great concern since they are primary producers of the aquatic food web. The aim of this study is to unravel the cytotoxicity of 2.5 μg mL−1 unlabelled amino-modified polystyrene beads of different sizes (0.5 and 2 μm) on the marine diatom Chaetoceros neogracile. In addition to traditional growth and photosynthesis endpoints, several physiological and biochemical parameters were monitored every 24 h in C. neogracile cells by flow cytometry during their...
Tipo: Text
Palavras-chave: Microalgae; Microplastic; Flow cytometry; Cytotoxicity; Scanning electron microscopy.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00492/60415/63850.pdf

Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis
Provedor de dados: 5
Autores: Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C.
Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity...
Tipo: Text
Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf

Bioactive Bromotyrosine-Derived Alkaloids from the Polynesian Sponge Suberea ianthelliformis
Provedor de dados: 5
Autores: El-demerdash, Amr; Moriou, Celine; Toullec, Jordan; Besson, Marc; Soulet, Stephanie; Schmitt, Nelly; Petek, Sylvain; Lecchini, David; Debitus, Cecile; Al-mourabit, Ali.
Herein, we describe the isolation and spectroscopic identification of eight new tetrabrominated tyrosine alkaloids 2–9 from the Polynesian sponge Suberea ianthelliformis, along with known major compound psammaplysene D (1), N,N-dimethyldibromotyramine, 5-hydroxy xanthenuric acid, and xanthenuric acid. Cytotoxicity and acetylcholinesterase inhibition activities were evaluated for some of the isolated metabolites. They exhibited moderate antiproliferative activity against KB cancer cell lines, but psammaplysene D (1) displayed substantial cytotoxicity as well as acetylcholinesterase inhibition with IC50 values of 0.7 μM and 1.3 μM, respectively.
Tipo: Text
Palavras-chave: Brominated tyrosine alkaloids; Suberea ianthelliformis; Cytotoxicity; Acetylcholinesterase inhibition.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00439/55076/56506.pdf

Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line
Provedor de dados: 77
Autores: Montiel-Eulefi,E; Jara,F; Toro,C; Garcés,M; Leal,P.
Poly (L-lactic acid) (PLA) nanoparticles have the approval of the main institutions for drugs administration and therapeutics. However, the use of lactic acid polymer is controversial because lactic acid has been proposed as an energy source for cancer cells. The aim of this study was to evaluate the cytotoxic, apoptotic and cell cycle properties of PLA and CuSO4-loaded PLA biodegradable nanoparticles on MKN-45 gastric adenocarcinoma cell line. PLA nanoparticles for the delivery of the anticancer active principle CuSO4 were obtained using the double emulsion method. PLA and CuSO4 loaded PLA nanoparticles were morphologically characterized and their size determined using transmission electron microscopy (TEM). The cytotoxicity of this drug delivery system...
Tipo: Journal article
Palavras-chave: Copper loaded nanoparticles; Poly-lactic acid; Adenocarcinoma MKN45cells; Cytotoxicity; Cell cycle.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100011

Effects of Malathion on Cellularity and Sperm Differentiation in Testis and Epididymis of Adult Rats
Provedor de dados: 77
Autores: Espinoza-Navarro,O; Bustos-Obregón,E.
Malathion is an organophosphorous insecticide, used worldwide for pest and disease control; however, it could also affect the reproductive patterns of several species. The aim of this study was to determine the effects of malathion in the cellularity and sperm differentiation in testis and epididymis of rats. Twenty adult male Sprague Dawley rats were divided into a malathion-treated group (n=10, dose of 170 mg/kg via subcutaneous injection for a period of 13 days) and control group (n=10, injected only with normal saline). After treatments, the rats were sacrificed by regulated euthanasia and assessed for sperm count in testis and epididymis and epididymal teratospermia degree. The results showed a significant decrease in body, testicular and epididymal...
Tipo: Journal article
Palavras-chave: Reproduction; Rat; Malathion; Cytotoxicity.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100020

Effect of Methamidophos on the Morphology and Male Reproductive Parameters of the Earthworm Eisenia foetida
Provedor de dados: 77
Autores: Espinoza-Navarro,Omar; Ferreira,Carolina; Bustos-Obregón,Eduardo.
The earthworm Eisenia foetida (Annelida, Oligochaeta) is an excellent biosentinel model organism for assessing potential toxicological risks by xenobiotics in terrestrial ecosystems. Methamidophos is an organophosphorous insecticide widely used to control agricultural pests. Its main action is due to inhibition of the enzyme acetylcholinesterase. The aim of this study was to evaluate the toxicity of methamidophos on the morphology and male parameters in Eisenia foetida. After determining the LD50 for methamidophos (85.34 mg/kg soil), five groups were formed, including control subjects, and sprayed with tap water. Methamidophos treated groups were subjected to doses of 1/10, 1/6, 1/3 and 2/3 of the LD50. Each group was analyzed at day 1, 6, 14 and 30...
Tipo: Journal article
Palavras-chave: Reproduction; Eisenia foetida; Methamidophos; Cytotoxicity.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300051

Organophosphorous Pesticides: Their Effects on Biosentinel Species and Humans. Control and Application in Chile
Provedor de dados: 77
Autores: Espinoza-Navarro,Omar; Ponce-LaRosa,Carlos; Bustos-Obregón,Eduardo.
The environment is negatively affected by the increasing accumulation of both natural and man-made waste and by-products. Organophosphorous pesticides -malathion, diazinon and methamidophos- are used worldwide in pest control. The aim of this report is to review the effects of organophosphates on the male reproductive tract of mice, rats and earthworms, and to evaluate their projection into the human population. Assessing failures in the male reproductive system is an excellent in vivo biomarker to determine the level of toxicity of suspected pollutants. In rodents organophosphates cause decreased testicular weight and sperm density, abnormal tubular plugging and increased teratozoospermia. In earthworms they cause a significant decrease in body weight and...
Tipo: Journal article
Palavras-chave: Organophosphates; Cytotoxicity; Biomonitoring; Reproductive Health; Occupational Exposure.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300041

SUBLETHAL DOSES OF MALATHION ALTER MALE REPRODUCTIVE PARAMETERS OF Eisenia foetida
Provedor de dados: 77
Autores: Espinoza-Navarro,Omar; Bustos-Obregón,Eduardo.
Malathion is an organophosphorate insecticide whose main acute poisonous effect is the inhibition of the enzyme acetylcholinesterase. This pesticide is widely used and has been reported to damage mice spermatogenesis. The objective of this work was to evaluate the toxic risk of malathion, earthworm Eisenia foetida (Annelida, Oligochaeta) like biological model was used and testicles and spermatozoa of animal controls and treaties were analyzed. Earthworms with developed clitellum were used and they were dewed with sublethal single doses of 0, 80, 150, 300 and 600mg of malathion for kilogram of soil (LD50 = 880mg/kg dry soil). Control groups were added only distilled water. Each group was analyzed 1, 5, 15 and 30 days post treatment. The results observed in...
Tipo: Journal article
Palavras-chave: Eisenia foetida; Malathion; Cytotoxicity; Spermatozoa; Reproductive organs.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400010

Cytological and cytogenic effects induced by thiabendazole on Allium cepa root meristem
Provedor de dados: 46
Autores: Andrioli,Nancy B; Mudry,Marta D.
The toxicity and genotoxicity of thiabendazole (TBZ) in its commercial formulation Foldan® was studied in Allium cepa meristematic cells at concentrations ranging between 10 and 250 µg / ml. Our results show that exposure to TBZ induces a significant increase in the frequency of anaphase telophase chromosomal aberrations, micronuclei and binucleated cells at all the concentrations of TBZ assayed. The frequency of anaphase telophase aberrations was observed to reach a plateau at the concentration of 50 µg / ml of TBZ, which could be probably due to TBZ mitotic toxicity manifested at concentrations of 100 and 250 µg / ml. An increase was also observed in the rate of metaphase and anaphase, which was, in turn, indicative of alterations in chromatid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Cytotoxicity; Mitotic index; Micronuclei; Binucleates; Thiabendazole.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000200002

HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic
Provedor de dados: 46
Autores: Andrioli,N.B; Chaufan,G; Coalova,I; Ríos de Molina,M.C; Mudry,M.D.
Early detection of toxic events induced by xenobiotics is necessary for a proper assessment of human risk after the exposure to those agents. The aim of this work was to evaluate the cell line HEp-2 as an experimental model to determine the genotoxic effects of sodium arsenate. To this end, we determined the metabolic activity cells by the MTT test on seven concentrations of arsenate that range from 27 to 135,000 &amp;#956;M, obtaining the median lethal concentration (LC50), the lowest observed effect concentration (LOEC), and the not observed effect concentration (NOEC) of sodium arsenate at 24 h of exposition. According to the cytotoxic response obtained, we evaluated the genotoxic effect of the 27 and 270 &amp;#956;M concentrations by using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Genotoxicity; Glutathione; HEp-2 cell line.
Ano: 2017
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332017000300002

Cytocompatibility, cytotoxicity and genotoxicity analyses of dental implants
Provedor de dados: 46
Autores: Reigosa,M.; Labarta,V.; Molinari,G.; Bernales,D.
Different materials are used for prostheses in dental medicine, though titanium is the most frequent. In the present report, several commercial dental implants and their potential capacity to integrate to bone tissues have been analysed by means of cytotoxicity, genotoxicity and cytocompatibility techniques. Cultures from osteosarcoma-derived UMR 106 cell line were used for bioassays since they showed several osteoblast properties. Samples of dental implants provided by B&amp;M national company (ASTM titanium 8348 GR2, acid-etched surface) were compared with some others commercialized by foreign firms in Argentina (Nobel: anodic-oxidized surface, and 3i: sandblasted surface). Using the neutral red uptake test, lysosome and alkaline phosphatase enzymatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Cytocompatibility; Single cell electrophoresis; Osteoblast.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332008000100007

Cytotoxic and apoptotic effects of scorpion Leiurus quinquestriatus venom on 293T and C2C12 eukaryotic cell lines
Provedor de dados: 80
Autores: Omran,M. A. A.
Scorpion venom toxicity is of major concern due to its influence on human activities and public health. The cytotoxicity and apoptosis induced by scorpion L. quinquestriatus venom on two established eukaryotic cell lines (293T and C2C12) were analyzed. Both cultured cell lines were incubated with varying doses (10, 20, and 50 µg/ml) of scorpion venom in serum free medium (SFM) for 0.5, 1, 2, 4, and 8 hours at 37°C. The percentage of total lactate dehydrogenase (LDH) released in the culture during venom incubation was used as an index of cell damage. Control culture was treated with an equal amount of SFM. Cell injury was recognized morphologically and apoptosis was researched by a Fluorescing Apoptosis Detection System using the principle of TUNEL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Leiurus quinquestriatus; Cytotoxicity; Apoptosis; LDH.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200008

Cytotoxicity of Southeast Asian snake venoms
Provedor de dados: 80
Autores: Jamunaa,A; Vejayan,J; Halijah,I; Sharifah,SH; Ambu,S.
Cytotoxicity of venoms from eleven medically important snakes found in Southeast Asia (Naja kaouthia, Naja siamensis, Naja sumatrana, Ophiophagus hannah, Bungarus candidus, Bungarus fasciatus, Enhydrina schistosa, Calloselasma rhodostoma, Trimeresurus purpureomaculatus and Tropidolaemus sumatranus) was determined, based on the MTS cytotoxicity assay, which determines the survival of viable cells in monolayer MDCK and Vero cell cultures upon exposure to the snake venoms. Snake venom toxicity was expressed as the venom dose that killed 50% of the cells (CTC50) under the assay conditions. Venoms of C. rhodostoma (2.6 µg/mL, 1.4 µg/mL) and O. hannah were the most cytotoxic (3.8 µg/mL, 1.7 µg/mL) whereas N. siamensis venom showed the least cytotoxicity (51.9...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Snake venom; Vipers; Elapids; Kraits.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200004

Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity
Provedor de dados: 80
Autores: Erdeş,Efe; Doğan,Tuğba Somay; Coşar,İlhan; Danışman,Tarık; Kunt,Kadir Boğaç; Şeker,Tamay; Yücel,Meral; Özen,Can.
Background Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs.Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.Methods In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxic and antimicrobial effects were evaluated on a breast cancer cell line (MCF-7) and various bacterial and fungal species.Results Proteins make up approximately half of the dry weight of L. abdullahbayrami crude venom. Microfluidic capillary electrophoresis indicated the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Toxin; Peptide; Leiurus abdullahbayrami; Microfluidic capillary; Electrophoresis; Peptidomics; Venomics; Cytotoxicity; Antimicrobial activity; Turkey.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200338

Cytotoxic and inflammatory potential of a phospholipase A2 from Bothrops jararaca snake venom
Provedor de dados: 80
Autores: Cedro,Rafhaella C. A.; Menaldo,Danilo L.; Costa,Tássia R.; Zoccal,Karina F.; Sartim,Marco A.; Santos-Filho,Norival A.; Faccioli,Lúcia H.; Sampaio,Suely V.
Abstract  Background: Snake venom phospholipases A2 (PLA2s) have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. This work aimed at the isolation and functional characterization of a PLA2 isolated from Bothrops jararaca venom, named BJ-PLA2-I.  Methods and Results: For its purification, three consecutive chromatographic steps were used (Sephacryl S-200, Source 15Q and Mono Q 5/50 GL). BJ-PLA2-I showed acidic characteristics, with pI~4.4 and molecular mass of 14. 2 kDa. Sequencing resulted in 60 amino acid residues that showed high similarity to other Bothrops PLA2s, including 100% identity with BJ-PLA2, an Asp49 PLA2 previously isolated from B. jararaca venom. Being an Asp49 PLA2, BJ-PLA2-I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops jararaca; Phospholipase A2; Inflammation; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100324

Cytotoxic activity of Androctonus australis hector venom and its toxic fractions on human lung cancer cell line
Provedor de dados: 80
Autores: Béchohra,Louisa; Laraba-Djebari,Fatima; Hammoudi-Triki,Djelila.
Abstract  Background: Several studies have showed that animal venoms are a source of bioactive compounds that may inhibit the growth of cancer cells, which makes them useful agents for therapeutic applications. Recently, it was established that venom toxins from scorpions induced cytotoxic, antiproliferative and apoptogenic effects on cancer cells. Therefore, the present study aims to investigate the cytotoxic activity of Androctonus australis hector (Aah) scorpion venom and its toxic fractions (FtoxG-50 and F3) on NCI-H358 human lung cancer cells.  Methods: The cytotoxic and antiproliferative activities were estimated using MTT assay, lactate dehydrogenase release and clonogenic assays. Apoptosis was evaluated by Hoechst 33258 staining, DNA fragmentation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aah venom; Cytotoxicity; F3 fraction; Apoptosis; Lung cancer cells; Oxidative stress.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100322

Potentiation of a novel palladium (II) complex lethality with bee venom on the human T-cell acute lymphoblastic leukemia cell line (MOLT-4)
Provedor de dados: 80
Autores: Safaeinejad,Zahra; Mohammad Nabiuni,; Nazari,Zahra.
Background : Although honeybee venom (BV) has been reported to induce apoptosis in different types of cancerous cells, its synergistic effects with customary anti-cancer drugs remain largely unknown. In the present study, we evaluated the cytotoxic effect of BV alone (as a natural product) and the synergistic cytological effects of this component in combination with [Pd (bpy) (Pi-Pydtc)]NO3 - a novel palladium complex on human T-cell lymphoblastic leukemia cells. To investigate the cytotoxic effect of the BV alone and in combination with palladium complex on MOLT-4 cells MTT assay was performed. In order to determine the apoptotic effects of BV separately and in combination with Pd (II) complex on these cells and its ability to induce apoptosis,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Bee venoms; Cytotoxicity; MOLT-4 cell line; Pd (II) complex.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100315

Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells
Provedor de dados: 80
Autores: Shahbazi,Benyamin; Najafabadi,Zahra Salehi; Goudarzi,Hamidreza; Sajadi,Mahnaz; Tahoori,Fatemeh; Bagheri,Masoumeh.
ABSTRACT  Background:  Several studies have pointed out that certain snake venoms contain compounds presenting cytotoxic activities that selectively interfere with cancer cell metabolism. In this study, Pseudocerastes persicus venom and its fractions were investigated for their anticancer potential on lung cancer cells.  Methods: Lung cancer cells (A549) and normal fibroblast cells (Hu02) were treated with the P. persicus venom and its HPLC fractions and the cell cytotoxic effects were analyzed using MTT and lactate dehydrogenase release assays. Apoptosis was determined in venom-treated cell cultures using caspase-3 and caspase-9 assay kits.  Results:  The treatment of cells with HPLC fraction 21 (25-35 kDa) of P. persicus venom resulted in high LDH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudocerastes persicus venom; Persian horned viper; HPLC fractions; Cytotoxicity; Apoptosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100319

Biochemical characterization and cytotoxic effect of the skin secretion from the red-spotted Argentina frog Argenteohyla siemersi (Anura: Hylidae)
Provedor de dados: 80
Autores: Fusco,Luciano S.; Cajade,Rodrigo; Piñeiro,Jose M.; Torres,Ana M.; Silva,Igor R. F. da; Hyslop,Stephen; Leiva,Laura C.; Pimenta,Daniel C.; Bustillo,Soledad.
Abstract  Background: Argenteohyla siemersi (red-spotted Argentina frog) is a casque-headed tree frog species belonging to the Hylidae family. This species has a complex combination of anti-predator defense mechanisms that include a highly lethal skin secretion. However, biochemical composition and biological effects of this secretion have not yet been studied.  Methods: The A. siemersi skin secretion samples were analyzed by mass spectrometry and chromatographic analysis (MALDI-TOF/MS, RP-HPLC and GC-MS). Proteins were also studied by SDS-PAGE. Among the biological activities evaluated, several enzymatic activities (hemolytic, phospholipase A2, clotting, proteolytic and amidolytic) were assessed. Furthermore, the cytotoxic activity (cytolysis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Argenteohyla siemersi; Hylidae; Skin secretion; Tree frog; Cytotoxicity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100306

Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
Provedor de dados: 80
Autores: Bustillo,S; Lucero,H; Leiva,LC; Acosta,O; Kier Joffé,EB; Gorodner,JO.
Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 µg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50: 2 µg/mL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops alternatus; Bothrops diporus; Cytotoxicity; C2C12; Apoptosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100004

Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)
Provedor de dados: 80
Autores: Haddad Junior,Vidal; Zara,Fernando; Marangoni,Sergio; Toyama,Daniela de Oliveira; Souza,Alex Jardelino Felizardo de; Oliveira,Simone Cristina Buzzo de; Toyama,Marcos Hikari.
Background: Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects ofO. sambaquiensis venom as well as clinical manifestations observed in affected patients. Crude protein extracts were prepared from the tentacles of animals; peptides and proteins were sequenced and submitted to circular dichroism spectroscopy. Creatine kinase, cytotoxicity and hemolytic activity were evaluated by specific methods.Results: We identified two novel cytolysins denominated oshem 1 and oshem 2 from the tentacles of this jellyfish. The cytolysins presented the amino...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olindias sambaquiensis; Hemolytic; Myonecrosis; Cytotoxicity; Cytolysin; Cnidaria venom.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200316

Cytotoxic and pro-apoptotic action of MjTX-I, a phospholipase A2 isolated from Bothrops moojeni snake venom, towards leukemic cells
Provedor de dados: 80
Autores: Benati,Rogério Bodini; Costa,Tássia Rafaela; Cacemiro,Maira da Costa; Sampaio,Suely Vilela; Castro,Fabíola Attié de; Burin,Sandra Mara.
Abstract  Background: Chronic myeloid leukemia (CML) is a BCR-ABL1+ myeloproliferative neoplasm marked by increased myeloproliferation and presence of leukemic cells resistant to apoptosis. The current first-line therapy for CML is administration of the tyrosine kinase inhibitors imatinib mesylate, dasatinib or nilotinib. Although effective to treat CML, some patients have become resistant to this therapy, leading to disease progression and death. Thus, the discovery of new compounds to improve CML therapy is still challenging. Here we addressed whether MjTX-I, a phospholipase A2 isolated from Bothrops moojeni snake venom, affects the viability of imatinib mesylate-resistant Bcr-Abl+ cell lines.  Methods: We examined the cytotoxic and pro-apoptotic effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic myeloid leukemia; Bcr-Abl; Phospholipase A2; MjTX-I; Bothrops moojeni; Apoptosis; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100330

Biochemical and morphological analysis of cell death induced by Egyptian cobra (Naja haje) venom on cultured cells
Provedor de dados: 80
Autores: Omran,M. A. A.; Fabb,S. A.; Dickson,G.
We investigated the in vitro process of cell death caused by Egyptian cobra venom on primary human embryonic kidney (293T) and mouse myoblast (C2C12) cell lines. The aim of these studies was to provide further information about triggering cell death, and suggest methods for eliminating unwanted cells, such as tumour cells. Both cell lines were treated with 10, 20, and 50 m g/ml of Egyptian cobra (Naja haje) venom in serum free media (SFM) and incubated for 8 hours. Total activities of the lactate dehydrogenase (LDH) and creatine kinase (CK) released in the culture during venom incubation were used as an indicator of the venom in vitro cytotoxicity. Cell injury was morphologically recognized and apoptosis determined by a Fluorescing Apoptosis Detection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cobra venom; Cytotoxicity; Apoptosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300004

DNA fragmentation and membrane damage of bocachico Prochilodus magdalenae (Ostariophysi: Prochilodontidae) sperm following cryopreservation with dimethylsulfoxide and glucose
Provedor de dados: 83
Autores: Martínez,José Gregorio; García,Víctor Atencio; Carrasco,Sandra Pardo.
The endangered bocachico Prochilodus magdalenae is a native freshwater fish of Colombia, the most captured species locally and one of the most important species for ex-situ conservation (germplasm banks). The aim of this study was to examine the effect of three concentrations of Dimethylsulfoxide (DMSO) (5%, 10%, 15%) and three of glucose (305, 333, 361 mM) in the extender on spermatic DNA fragmentation (F-DNA) (by Halomax®, Chromatin dispersion) and membrane damage (D-Me) (by eosin-nigrosin staining). After assessment of sperm quality by computer analysis of motility, one part of semen from males was diluted separately with three parts of extender and filled into 0.5 ml straws. Freezing was carried out in liquid nitrogen vapor dry shipper for 30 minutes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characiformes; Cryobiology; Cytotoxicity; Genotoxicity; Germplasm.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300011

Polar Alkaloids from the Caribbean Marine Sponge Niphates digitalis
Provedor de dados: 20
Autores: Regalado, E.L.; Mendiola, J.; Laguna, A.; Nogueiras, C.; Thomas, O.P.
A method involving flash chromatography, semi-preparative phenylhexyl RP HPLC-DAD-ELSD combined with analytic polar-RP HPLC-DAD, was applied to separate and purify six highly nitrogenated bases and a bicyclic amidine alkaloid, the major components of the marine sponge Niphates digitalis. Their structures were identified as 1,8-diazabicyclo[5.4.0]undec-7- ene (1), deoxycytidine (2), phenylalanine (3), adenosine (4), deoxyguanosine (5), adenine (6) and thymidine (7) on the basis of spectroscopic data analyses. This is the first report of these compounds in a marine sponge belonging to the Niphates genus and the first evidence of the presence of 1 from a natural source.
Tipo: Journal Contribution
Palavras-chave: Sponges; Cytotoxicity; Cytotoxicity; Sponges; Http://aims.fao.org/aos/agrovoc/c_34251; Http://aims.fao.org/aos/agrovoc/c_7321.
Ano: 2010
URL: http://hdl.handle.net/1834/3860

Ichthyotoxic, cytotoxic and antimicrobial activity of some sponges of the Colombian Caribbean
Provedor de dados: 20
Autores: Zea, S.; Medina, A.; Duque, C.
The icthyotoxic, cytotoxic and antimicrobial activities of aqueous, ethanol and chloroform extractsof 11 spongesfrom the Santa Marta región, Colombian Caríbbean.  were assayed. All studied sponges showed antimicrobial activity; 6 were ichthyotoxic and 7 were cytotoxic. Ircinia campana, Verongula rigida, Agelas conifera and Didiscus oxeata, were found to have activity in all three kinds of tests.
Tipo: Journal Contribution
Palavras-chave: Ichthyology; Cytotoxicity; Sponges; Cytotoxicity; Ichthyology; Sponges; Http://aims.fao.org/aos/agrovoc/c_34251; Http://aims.fao.org/aos/agrovoc/c_3788; Http://aims.fao.org/aos/agrovoc/c_7321.
Ano: 1986
URL: http://hdl.handle.net/1834/4045

Étude des saponines isolées d'une espèce d'holothurie &lt;Holothuria tubulosa&gt; de la lagune de Bizerte
Provedor de dados: 20
Autores: Tekitek, A.; Sellem, F.; Langar, H.; El Abed, A.; Louiz, I.
قمنا في هذا البحث باستخلاص عدد من الصابونيات من صنف من اصناف خيار البحر المتواجدة ببحيرة بنزرت وهي (اولوتيغيا تيبيلوزا)  &lt;tubulosa Holothuria&gt; وتمت دراسة هذه المواد المستخلصة حسب مرحلة النضوج الجنسي و تم كذلك اثبات ان هذا المستحضر ذو مفعول بيولوجي اذ تسبب في انحلال كريات الدم الحمراء
Tipo: Journal Contribution
Palavras-chave: Cadmium; Chemical extraction; Cytotoxicity; Saponins; Cadmium; Http://aims.fao.org/aos/agrovoc/c_1178.
Ano: 2003
URL: http://hdl.handle.net/1834/1129

Ensayos de actividad biológica y ecología química de extractos orgánicos de macroalgas del Caribe colombiano
Provedor de dados: 20
Autores: Díaz R., M.C.; Bula Meyer, G.; Zea, S.; Martínez, A.
This study evaluates the biological activity and possible deterrance played by organic crude extracts from six Phaeophyta and two Rhodophyta algae from Southern Caribbean. Bioessays were carried out using concentrations equivalent to those present in algae tissue. Species in general did not show high citotoxic activity. However Dictyota pulchella, showed a delay in the mitotic process for cells stage XVI. Extracts from Dictyota bartayresiana, D. pulchella, Sargassum cymosum and Cladophyllum schnetteri, inhibited S. partitus feeding under laboratory conditions. Nontheless, only extracts from D. bartayresiana and C. schnetteri showed this activity in the field.
Tipo: Journal Contribution
Palavras-chave: Algae; Cytotoxicity; Cytotoxicity; Algae; Http://aims.fao.org/aos/agrovoc/c_34251; Http://aims.fao.org/aos/agrovoc/c_258.
Ano: 2006
URL: http://hdl.handle.net/1834/1985

Capacidad antimitotica de extractos de esponjas marinas del Caribe colombiano
Provedor de dados: 20
Autores: Mora-Christancho, J.; Zea, S.; Santos-Acevedo, M.; Newmark-Umbreit, F.
La determinación de actividad antimitótica de extractos de organismos marinos genera expectativas para la obtención de sustancias con potencial anticancerígeno a partir de fuentes alternativas. Se evaluó la actividad antimitótica in vitro de extractos orgánicos crudos de 15 esponjas de la costa Caribe colombiana, en embriones del erizo de mar Lytechinus variegatus. El 80% de las especies (Spirastrella coccinea, Myrmekioderma rea, Iotrochota imminuta, Halichondria sp., Petromica ciocalyptoides, Cinachyrella kuekenthali, Biemna cribaria, Oceanapia peltata, Xestospongia proxima, Oceanapia bartschi, Polymastia tenax y Dragmacidon reticulata) mostró un nivel de actividad fuerte inhibiendo la divisiones mitóticas desde el primer estadio celular durante la...
Tipo: Journal Contribution
Palavras-chave: Cytotoxicity; Cytotoxicity; Http://aims.fao.org/aos/agrovoc/c_34251.
Ano: 2007
URL: http://hdl.handle.net/1834/3472

Characterization and Digestibility of Detoxified Castor Oil Meal for Japanese Quails
Provedor de dados: 89
Autores: Santos,PA dos; Ludke,MCMM; Ludke,JV; Rabello,CBV; Santos,MJB dos; Torres,TR.
ABSTRACT These experiments were performed to determine the chemical composition, coefficients of nutrient and energy metabolizability, amino acid composition, and cytotoxicity of different castor oil meals subjected to different detoxification processes and added to the diet of Japanese quails. In the trial, 180 46-d-old female Japanese quails were distributed according to a completely randomized design into five treatments and with replicates of six bird each. The treatments consisted of following detoxification methods of castor oil meal: Castor oil meal A (CMA) - recovery in alcohol at 80 °C for 20 minutes and drying at 80 °C; castor oil meal B (CMB) and C (CMC) - recovery in alcohol at 80 °C for 6 minutes, neutralization with 5% NaOH, and drying under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative food; Poultry; Cytotoxicity; Digestibility; Energy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015001000065

In vitro antioxidant, antitumor and leishmanicidal activity of riparin A, an analog of the Amazon alkamides from Aniba riparia (Lauraceae)
Provedor de dados: 36
Autores: ARAÚJO,Éverton José Ferreira de; LIMA,Layana Karine Farias; SILVA,Oskar Almeida; JÚNIOR,Luís Mário REZENDE; GUTIERREZ,Stanley Juan Chavez; CARVALHO,Fernando Aécio de Amorim; LIMA,Francisco das Chagas Alves; PESSOA,Cláudia; FREITAS,Rivelilson Mendes de; FERREIRA,Paulo Michel Pinheiro.
ABSTRACT Aniba riparia (Lauraceae) is an important medicinal plant found in the Amazon region and presents alkaloids of the type alkamide known as riparins. Riparin A is structurally represented as the fundamental core of all Amazon riparins. This work aimed to assess the in vitro antioxidant, antitumor and antileishmanial effects of riparin A. Riparin A presented weak antioxidant capacity by tecniques of DPPH• (EC50 of 296.2 μg mL-1) and ABTS•+ (EC50 of 450.1 μg mL-1), showed moderate activity against colon carcinoma (HCT-116: IC50 of 21.7 μg mL-1) and leishmanicidal activity on promastigotes of L. amazonensis (IC50 of 307.0 ± 79.6, 193.7 ± 44.3 and 81.8 ± 11.2 μg mL-1, respectively, after 24, 48 and 72 h of incubation). Then, in addition to its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioprospecting; Chemoprevention; Cytotoxicity; Antiparasitic drug.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000300309

Screening of plants found in Amazonas State for lethality towards brine shrimp
Provedor de dados: 36
Autores: QUIGNARD,Etienne Louis Jacques; POHLIT,Adrian Martin; NUNOMURA,Sergio Massayoshi; PINTO,Ana Cristina da Silva; SANTOS,Elba Vieira Mustafa dos; MORAIS,Sabrina Kelly Reis de; ALECRIM,Alexandre Mascarenhas; PEDROSO,Andreza Cristiana da Silva; CYRINO,Barbara Rachel Brito; MELO,Christiane Santana de; FINNEY,Ellen Kathryn; GOMES,Erika de Oliveira; SOUZA,Katiuscia dos Santos de; OLIVEIRA,Laura Cristina Pereira de; DON,Luciana de Castro; SILVA,Luiz Francisco Rocha e; QUEIROZ,Maria Mireide Andrade; HENRIQUE,Marycleuma Campos; SANTOS,Mirian dos; PINTO,Patrícia de Souza; SILVA,Suniá Gomes.
226 methanol and water extracts representing 74 mainly native plant species found in Amazonas State, Brazil, were tested at a standard concentration of 500 μg/mL for lethality towards larvae of the brine shrimp species Artemia franciscana. Several cytotoxic plant species were identified in this work: Aspidosperma marcgravianum, A. nitidum, Croton cajucara, Citrus limetta, Geissospermum argenteum, Minquartia guianensis, Piper aduncum, P. amapense, P. capitarianum, P. tuberculatum and Protium aracouchini. The results were analyzed within the context of the available traditional knowledge and uses for these plants.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemia franciscana; Cytotoxicity; Amazonian plants.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672003000100093

Cytotoxic evaluation of essential oil from Zanthoxylum rhoifolium Lam. leaves
Provedor de dados: 36
Autores: Silva,Saulo Luis da; Figueiredo,Patrícia Maria; Yano,Tomomasa.
Zanthoxylum rhoifolium Lam is a plant popularly used as antimicrobial, for malaria and inflammatory treatment. The essential oil of Z. rhoifolium was extracted and its cytotoxic effects against HeLa (human cervical carcinoma), A-549 (human lung carcinoma), HT-29 (human colon adenocarcinoma), Vero (monkey kidney) cell lines and mice macrophages were evaluated. Some of the terpenes of its essential oil (ß-caryophyllene, alpha-humulene, alpha -pinene, myrcene and linalool) were also tested to verify their possible influence in the oil cytotoxic activity. The results obtained permitted to confirm that the essential oil is cytotoxic against tumoral cells (CD50 = 82.3, 90.7 and 113.6 µg/ml for A-549, HeLa e HT-29 cell lines, respectively), while it did not show...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zanthoxylum; Essential oil; Cytotoxicity; SS-caryophyllene; Alpha-humulene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672007000200015

Toxicity of the goji berry fruit associated with artificial excipients and dried without additives
Provedor de dados: 3
Autores: Moura, Débora Dayane Araújo de; Veloso, Cleidiane Josefa dos Santos; Oliveira, Valtânia Ana de; Silva, Maria Eduarda Sousa e; Peron, Ana Paula.
 The aim of this study was to evaluate the cytotoxic and genotoxic potential of goji berry fruit-based pharmaceutical powders obtained from three pharmaceutical laboratories. The product A was tested at concentrations of 0.012; 0.025 and 0.05 g mL-1, and B and C at concentrations 0.02; 0.04 and 0.08 g mL-1. It was also evaluated the tea of the dried goji berry fruit (non-additives) in the concentrations 0.035; 0.07 and 0.14 g mL-1 for comparison to the results obtained with powdered goji berry. Tea concentrations in the two exposure times did not cause inhibition of cell division nor cellular alterations to meristem tissues. For the industrialized goji products, all concentrations analyzed caused significant antiproliferative effect to the tissues...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycium barbarum L.; Natural pharmaceutical product; Cytotoxicity; Genotoxicity; Meristematic tissue.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37844

Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L.
Provedor de dados: 3
Autores: Santana, Gleuvânia Marques; Sousa, Josefa Janaína do Anjos; Peron, Ana Paula.
This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p &lt; 0.05). From the results, we observed that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tecnolologia de alimentos  citogenética azo dye; Cell division; Cellular aberrations; Cytotoxicity.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119

Processed fruit juice ready to drink: screening acute toxicity at the cellular level
Provedor de dados: 3
Autores: Silva, Erick Leal da; Sales, Ila Monize Sousa; Santos, Fabelina Karollyne Silva dos; Peron, Ana Paula.
The present study evaluated the acute toxicity at the cellular level of processed juice ready for consumption Orange and Grape flavors, produced by five companies with significant influence on the food market of South American countries, especially in Brazil. This evaluation was performed in root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, directly with marketed liquid preparations. Based on the results, it was found that fruit juices, of all companies considered, promoted significant antiproliferative effect to root meristems at the exposure time of 24 hours and resulted in at both exposure times, statistically significant number of mitotic spindle changes and chromosomal breaks. Therefore, under the study conditions, all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Industrialized juice; Cytotoxicity; Genotoxicity; Mutagenicity; Meristem tissue.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33699

Toxicity at the cellular level of artificial synthetic flavorings
Provedor de dados: 3
Autores: Sales, Ila Monize Sousa; Oliveira, Jussara Damascena de; Santos, Fabelina Karollyne Silva dos; Feitoza, Lidiane de Lima; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
The goal of the present study was to evaluate the cytotoxicity and genotoxicity of artificial synthetic flavoring agents Cookie and Tutti-frutti. To this end, root meristem cells of Allium cepa L. were exposed to these substances in exposure times of 24 and 48 hours using individual doses of 0.3; 0.6 and 0.9 mL and doses combined as follows: 0.3 mL + 0.3 mL; 0.6 mL and 0.9 mL + 0.6 mL + 0.9 mL. After applying the treatments, root meristems were fixed, hydrolyzed, stained and analyzed a total of 5,000 cells using an optical microscope to evaluate each dose and combined treatment. All three doses of Cookie flavoring and combined treatments significantly inhibited cell division of the tissue studied. Doses of Tutti-Frutti caused no change in cell division...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30751

Toxicity in food flavorings at the cellular level associated with each other at different doses
Provedor de dados: 3
Autores: Silva, Sara Iolanda Oliveira da; Santana, Gleuvânia Marques; Sales, Ila Monize Sousa; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
This study aimed to examine the cytotoxicity and genotoxicity of synthetic flavorings, nature identical, Chocolate, Strawberry and Condensed Milk. This evaluation was performed in root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL, in combination, in which one of the three doses of a flavoring was combined with a different dose of one of the two other flavor additives studied. Roots were fixed in Carnoy’s solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and then analyzed, under light microscopy, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. All the treatments with combinations between the flavorings Chocolate/Strawberry and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aroma and flavor additives; Cytotoxicity; Genotoxicity  A. cepa Citogenética.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30475

An anti-dengue and anti-herpetic polysulfated fraction isolated from the coenocytic green seaweed Caulerpa cupressoides inhibits thrombin generation in vitro
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; Eloy, Ygor Raphael Gomes; Vanderlei, Edfranck de Souza Oliveira; Cavalcante, Jéssica Figueiredo; Romanos, Maria Tereza Villela; Benevides, Norma Maria Barros.
The antiviral potency of Caulerpa cupressoides (Chlorophyta) sulfated polysaccharidic fractions (Cc-SP1→3) associated with thrombin generation (TG) assay is unknown. This study analyzed the structure of Cc-SP1 and its effects against herpes viruses (HSV-1 and HSV-2), dengue-virus (DENV-1), human-metapneumovirus (HMPV) and adenovirus (AdV) in Vero and mosquito C6/36 cell-lines and as inhibitor of TG in 60-fold diluted human plasma using continuous system. Infrared analysis indicated ulvan containing 11% sulfate, 40.16% total sugars and 6% uronic acid. Procedures of agarose/polyacrylamide gels electrophoresis revealed two major components presenting sulfate and non SPs, with molecular dispersion ranging from 8 to &gt; 100 kDa, respectively, as confirmed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Química de macromoléculas Bryopsidales; Marine glycans; Cytotoxicity; Viral infection; Thrombin. Bioprospecção.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28081

Cell death induced by tamoxifen in human blood lymphocytes cultivated in vitro - doi: 10.4025/actascibiolsci.v32i4.7015
Provedor de dados: 3
Autores: Oliveira, Naila Francis Paulo de; UNICAMP; Genari, Selma Candelária; UNICAMP; Dolder, Heidi; UNICAMP.
Many chemotherapeutic agents with a potential against solid tumors or leukemia can cause lymphopenia. Tamoxifen (TAM) is a synthetic non-steroidal anti-estrogen drug employed in female breast cancer treatment. The present study investigated the capacity of TAM to induce cell death in human lymphocytes cultivated in vitro. Lymphocytes were obtained from young (25-30 years; n = 3) and elderly women (58-77 years; n = 3) and cultivated for 24 or 48h, with or without TAM (20 &amp;micro;M). After the culture, cell viability, immunocytochemical response and ultrastructure were evaluated. TAM affected lymphocytes in a time- dependent manner, and cells obtained from elderly women were the most sensitive to TAM. Immunocytochemical analysis evidenced higher frequency...
Palavras-chave: 2.06.01.00-0 Apoptosis; Autophagy; Chemotherapeutic; Cytotoxicity; Morphology; Ultrastructure Citologia e Biologia Celular.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7015

Antibacterial and cytotoxicity activity in macroalgae extracts: perspectives for the use against pathogenic bacteria from shrimp farms (Litopenaeus vannamei)
Provedor de dados: 3
Autores: Silva, Giselle Cristina; Soares, Raquel Cavalcante; Carvalho, Fátima Cristiane Teles; Rocha, Rafael dos Santos; Vieira, Regine Helena Silva dos Fernandes; Sousa, Oscarina Viana de.
Some infections caused by pathogenic microorganisms might shows high prevalence in farmed shrimp environments, compromising production and causing economic losses. Therefore, the search for compounds with antibiotic activity has become intensive, following the record of new antimicrobial-resistant bacteria. The study of those bioactive compounds in marine macroalgae has produced satisfactory results, such as the discovery of antibacterial activity against multiresistant strains. Accordingly, this study aims to research antibiotic activity in macroalgae extracts of Chlorophyta, Phaeophyta and Rhodophyta found in the coast of Ceará and also to evaluate the cytotoxicity activity against bacterial strains (Vibrio sp.) from shrimp farms (Litopenaeus vannamei)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.12.02.00-1 bioactivity; Antibiotic; Cytotoxicity; Resistance. Microbiologia Aplicada.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40053

Assessment of the cytotoxic, genotoxic, and antigenotoxic activities ofCeltis iguanaea (Jacq.) in mice
Provedor de dados: 42
Autores: BORGES,FLAVIO F.V.; MACHADO,THIAGO C.; CUNHA,KÊNYA S.; PEREIRA,KARLA C.; COSTA,ELSON A.; PAULA,JOSÉ R. DE; CHEN-CHEN,LEE.
Ethnobotanical surveys of Cerrado native plants show that leaves of Celtis iguanaea (Jacq.) Sargent (Cannabaceae), popularly known in Brazil as “esporão de galo”, are used in folk medicine for body pain, asthma, cramps, poor digestion, urinary infection, kidney dysfunctions, as well as a stimulant and diuretic. This work aimed at evaluating possible C. iguanaea aqueous leaf extract (CALE) cytotoxicity, genotoxicity, and antigenotoxicity using the mouse bone marrow micronucleous test. To assess CALE genotoxicity, Swiss mice were orally treated with three different extract concentrations (100, 300, and 500 mgkg−1). To evaluate its antigenotoxicity, the same doses were used simultaneously with a single i.p. dose of mitomycin C (MMC, 4mg.kg−1). The frequencies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Genotoxicity; Mice; Medicinal plant.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300955

Synthesis and evaluation of arylamidine derivatives for new antimicrobial and cytotoxic activities
Provedor de dados: 42
Autores: MONTE,ZENAIDE S.; SILVA,AMANDA M.; LIMA,GLÁUCIA M.S.; SILVA,TERESINHA G. DA; MARQUES,KARLA M.R.; RODRIGUES,MARIA D.; FALCÃO,EMERSON P.S.; MELO,SEBASTIÃO J.
ABSTRACT A series of arylamidines 3a-j was designed, synthesized and investigated for antimicrobial activity. Structures of the compounds were confirmed by IR, 1H-NMR and 13C-NMR and a 2D spectroscopic study was performed. A preliminary screening of the antimicrobial tests clearly showed that three out of ten arylamidines, viz, 3f, 3g and 3i, were effective against all the gram-negative bacteria: Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella enteric; and against the yeast, candida albicans. Further, the Minimum Inhibitory Concentrations (MIC) against the bacteria and yeast were determined. All compounds 3a-d, 3f, 3g, 3i and 3j were also investigated for their low cytotoxic effects on tested cell lines. Compounds 3d and 3f were the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arylamidines; 2D spectroscopy; Gram-negative bacteria; Yeast; Cytotoxicity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301051

Saline extract from Malpighia emarginata DC leaves showed higher polyphenol presence, antioxidant and antifungal activity and promoted cell proliferation in mice splenocytes.
Provedor de dados: 42
Autores: BARROS,BÁRBARA R.S.; BARBOZA,BRUNO RAFAEL; RAMOS,BÁRBARA A.; MOURA,MAIARA C. DE; COELHO,LUANA C.B.B.; NAPOLEÃO,THIAGO HENRIQUE; CORREIA,MARIA TEREZA S.; PAIVA,PATRÍCIA MARIA G.; CRUZ FILHO,IRANILDO JOSÉ DA; SILVA,TÚLIO DIEGO DA; LIMA,CLÁUDIA S.A.; MELO,CRISTIANE M.L. DE.
Abstract: Currently, the research of new natural compounds with biological potential demonstrates great ethnopharmacological importance. In this study, we evaluated the biological properties promoted by saline extract from Malpighia emarginata DC leaves, whose objective is to evaluate the antioxidant, antimicrobial and cytotoxicity potential. Phytochemical characterization was performed by UPLC-MS chromatography to identify the chemical compounds. For the antioxidant potential, DPPH, ATT and FRAP methods were used. The antibacterial and antifungal tests were performed evaluating the MIC50, MIC90, CMB and CMF parameters. Moreover, antibiofilm action was evaluated. Cytotoxicity and proliferation were performed using splenocytes from Balb/c mice and were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioactive compounds; Cytotoxicity; Malpighia emarginata DC; Phytochemical.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100627

Cytotoxic and toxicological effects of phthalimide derivatives on tumor and normal murine cells
Provedor de dados: 42
Autores: FERREIRA,PAULO MICHEL PINHEIRO; COSTA,PATRICIA MARÇAL DA; COSTA,ARINICE DE MENEZES; LIMA,DAISY JEREISSATI BARBOSA; DRUMOND,RENATA ROSADO; SILVA,JURANDY DO NASCIMENTO; MOREIRA,DIOGO RODRIGO DE MAGALHÃES; OLIVEIRA FILHO,GEVÂNIO BEZERRA DE; FERREIRA,JAMILE MAGALHÃES; QUEIROZ,MARIA GORETTI RODRIGUES DE; LEITE,ANA CRISTINA LIMA; PESSOA,CLÁUDIA.
Eleven phthalimide derivatives were evaluated with regards to their antiproliferative activity on tumor and normal cells and possible toxic effects. Cytotoxic analyses were performed against murine tumors (Sarcoma 180 and B-16/F-10 cells) and peripheral blood mononuclear cells (PBMC) using MTT and Alamar Blue assays. Following, the investigation of cytotoxicity was executed by flow cytometry analysis and antitumoral and toxicological potential by in vivo techniques. The molecules 3b, 3c, 4 and 5 revealed in vitro cytotoxicity against Sarcoma 180, B-16/F-10 and PBMC. Since compound 4 was the most effective derivative, it was chosen to detail the mechanism of action after 24, 48 and 72 h exposure (22.5 and 45 µM). Sarcoma 180 cells treated with compound 4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Histological alterations; Murine cells; Phthalimide derivatives; Sarcoma 180.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100313

Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea
Provedor de dados: 42
Autores: Moura,Raniere M.; Melo,Arthur A.; Carneiro,Rômulo F.; Rodrigues,Cícera R.f.; Delatorre,Plínio; Nascimento,Kyria S.; Saker-Sampaio,Silvana; Nagano,Celso S.; Cavada,Benildo S.; Sampaio,Alexandre H.
Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Purification; Hemolysis; Cytotoxicity; Galactose.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973

Essential oil of Mitracarpus frigidus as a potent source of bioactive compounds
Provedor de dados: 42
Autores: Fabri,Rodrigo L.; Coimbra,Elaine S.; Almeida,Ana C.; Siqueira,Ezequias P.; Alves,Tânia M.A.; Zani,Carlos L.; Scio,Elita.
In our previous work (Fabri et al. 2009), we showed that different extracts of Mitracarpus frigidus had significant antibacterial, antifungal and leishmanicidal activities. In order to increase our knowledge about this species, this work assesses the chemical composition and the in vitro biological activity of its essential oil. Thus, the essential oil obtained by hydrodistillation of the aerial parts of M. frigidus was analyzed by GC/MS. Among several compounds detected, 11 were identified, being linalool and eugenol acetate the major components. The essential oil exhibited a moderate antibacterial effect against Staphyloccocus aureus, Bacillus cereus, Pseudomonas aeruginosa and Enterobacter cloacae (MIC 250 µg/mL). On the other hand, it showed a strong...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitracarpus frigidus; Essential oil; Antimicrobial; Antileishmanial; Cytotoxicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400021

Assessment of Duguetia furfuracea genotoxic and cytotoxic activity in bacteria and mice
Provedor de dados: 42
Autores: Silva,Carolina R.; Vieira,Pabline M.; Santos,Suzana C.; Chen-Chen,Lee.
Duguetia furfuracea (St. Hil.) Benth &amp;amp; Hook f. (1862), popularly known as "sofre-do-rim-quem-quer" and "araticum-seco", is a shrub of the Annonaceae family that occurs in several regions of Brazil. In folk medicine the infusion of its leaves and twigs is used to treat rheumatism and renal colic, whereas the seed powder is mixed with water to treat pediculosis. Previous studies have described biological activities of this plant with cytotoxic, antitumoral, trypanocidal, leishmanicidal, antiplasmodial and antiprotozoal effects. In the present work, genotoxicity and cytotoxicity of Duguetia furfuracea lyophilized leaf extract were evaluated using the prophage λ induction test (SOS-Inductest) and mouse bone marrow micronucleus test. Our results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Duguetia furfuracea; Genotoxicity; Micronucleus test; SOS-Inductest.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100015

Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
Provedor de dados: 42
Autores: BASTOS,ISLA V.G.A.; OLIVEIRA,TATIANE B. DE; RODRIGUES,MARIA D.; MILITÃO,GARDÊNIA C.G.; SILVA,TERESINHA G. DA; TURATTI,IZABEL C.C.; LOPES,NORBERTO P.; MELO,SEBASTIÃO J. DE.
ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Gas Chromatography; Phoradendron microphyllum; Phoradendron mucronatum.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991

Evaluation of cytogenotoxicity, antioxidant and hypoglycemiant activities of isolate compounds from Mansoa hirsuta D.C. (Bignoniaceae)
Provedor de dados: 42
Autores: PEREIRA,JOQUEBEDE R.; QUEIROZ,RAPHAEL F.; SIQUEIRA,ERLÂNIA A. DE; BRASILEIRO-VIDAL,ANA CHRISTINA; SANT’ANA,ANTÔNIO E.G.; SILVA,DANIEL M.; AFFONSO,PAULO R.A. DE MELLO.
ABSTRACT Mansoa hirsuta (Bignoniaceae) is a native plant from caatinga in Brazilian semiarid. This plant has been locally used as antimicrobial and hypoglycemiant agents, but their action mechanisms and toxicity remain largely unknown. Therefore, we evaluated the composition and antioxidant, cytoprotective and hypoglycemiant effects of raw extract, fractions and compounds from leaves of M. hirsuta. The cytogenotoxic effects of ursolic and oleanolic acids, the main phytotherapic components of this plant, were assessed. The raw extract and fractions presented steroids, saponins, flavonols, flavanonols, flavanones, xanthones, phenols, tannins, anthocyanins, anthocyanidins and flavonoids. The ethyl acetate fraction inhibited efficiently the cascade of lipid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Caatinga; Cytotoxicity; Hypoglycemiant; Phytochemistry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100317

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.
Provedor de dados: 42
Autores: EMSEN,BUGRAHAN; OZDEMIR,OZLEM; ENGIN,TUBANUR; TOGAR,BASAK; CAVUSOGLU,SEYDA; TURKEZ,HASAN.
Abstract: Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Genotoxicity; HPLC; Total antioxidant capacity; Total oxidative stress.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500621

Cytotoxic sesquiterpene lactones from the aerial parts of Inula aucheriana.
Provedor de dados: 42
Autores: Gohari,Ahmad Reza; Mosaddegh,Mahmoud; Naghibi,Farzaneh; Eslami-Tehrani,Bahara; Pirani,Atefeh; Hamzeloo-Moghadam,Maryam; Read,Roger W.
Inula aucheriana DC is a member of the family Asteraceae which is known to produce cytotoxic secondary metabolites noted as sesquiterpene lactones. In the present study, sesquiterpene lactones inuchinenolide B, 6-deoxychamissonolide (stevin) and 14-acetoxy-1β,5α,7αH-4β-hydroxy-guai-9(10),11(13)-dien-12,8α-olide were isolated from I. aucheriana. Inuchinenolide B and 14-acetoxy-1β,5α,7αH-4β-hydroxy-guai-9(10),11(13)-dien-12,8α-olide were further evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay to demonstrate cytotoxic activity with IC50 values of (56.6, 19.0), (39.0, 11.8), and (55.7, 15.3) μg/mL against HepG-2, MCF-7 and A-549 cells, respectively. The cytotoxic activity of the two evaluated sesquiterpene lactones...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Inula aucheriana; MTT assay; Sesquiterpene lactones.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200777

Antimicrobial, antioxidant and cytotoxicity potential of Manihot multifida (L.) Crantz (Euphorbiaceae)
Provedor de dados: 42
Autores: FABRI,RODRIGO L.; SÁ,DANIEL S. DE; PEREIRA,ANA PATRÍCIA O.; SCIO,ELITA; PIMENTA,DANIEL S.; CHEDIER,LUCIANA M.
Manihot multifida (L.) Crantz (Euphorbiaceae) is widely used in popular medicine for the treatment of infected wounds. This study evaluated thein vitro antioxidant and antimicrobial potential of this species against strains of Gram-positive and Gram-negative bacteria and fungi, known to cause infections in humans. The extracts showed minimal inhibitory concentration (MIC) varying from 39 to 2500 µg/mL for antimicrobial activity. The methanolic extract of fruits, aqueous and hexane extracts of leaves showed a very strong activity against Candida albicans(ATCC 18804) with MIC of 39 µg/mL. Furthermore, the methanolic extract of M. multifida leaves exhibited DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging potential with inhibitory concentration (IC50) values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial; Antioxidant; Candida albicans; Cytotoxicity; Manihot multifida.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100303

Acute Toxicity and Cytotoxicity Effect of Ethanolic Extract of Spondias tuberosa Arruda Bark: Hematological, Biochemical and Histopathological Evaluation
Provedor de dados: 42
Autores: BARBOSA,HUMBERTO M.; NASCIMENTO,JAILSON N. DO; ARAÚJO,THIAGO A.S.; DUARTE,FILIPE S.; ALBUQUERQUE,ULYSSES P.; VIEIRA,JEYMESSON R.C.; SANTANA,EDSON R.B. DE; YARA,RICARDO; LIMA,CLÁUDIA S.A.; GOMES,DAYANE A.; LIRA,EDUARDO C.
ABSTRACT Spondias tuberosa Arruda, popularly named as umbu, is native from savanna-like vegetation and widely used for medicinal purposes, however, the toxicological profile is not available yet. This study evaluated the phytochemical profile and acute toxicity and citoxicity of Ethanolic Extract of Spondias tuberosa Arruda Bark (EEStb) in hematological, biochemical and histopathological parameters. Female Wistar rats were divided into: control (C) and animal treated single doses of 300mg/Kg (EEStb300) or 2.000mg/kg body weight (ESStb2.000) of the EEStb. After 24 hours and 14 days from gavage, the behavior, hematological, biochemical and histopathological parameters were assayed. Cytotoxicity effect was evaluated on HEp-2 cell lines. Neither EEStb300 nor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute toxicity; Cytotoxicity; Phytomedicine; Safety; Spondias tuberosa Arruda; Toxicology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501993

Butein imparts free radical scavenging, anti-oxidative and pro-apoptotic properties in the flower extracts of Butea monosperma
Provedor de dados: 47
Autores: Sehrawat,Anuradha; Kumar,Vijay.
The flower of Butea monosperma (Lam.) (Fabaceae) has been used in traditional Indian medicine in the treatment of many ailments including liver disorders. To understand the pharmacological basis of its beneficial effects, the extracts of dried flowers in water, methanol, butanol, ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures (human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes). Butrin and butein -the active constituents of flower extracts- were used as reference molecules. The levels of cell injury markers like lactate dehydrogenase, glutathione and lipid peroxidation and primary antioxidant enzymes glutathione S-transferase and catalase were also measured. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Butrin; Cytotoxicity; Hepatoprotection; Methanol extract; Free radical scavenging.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200003

The cytotoxic and antileishmanial activity of extracts and fractions of leaves and fruits of Azadirachta indica (A Juss.)
Provedor de dados: 48
Autores: Carneiro,Sabrina M. P; Carvalho,Fernando A. A; Santana,Lorena C. L. R; Sousa,Alessian P. L; Neto,José M. M; Chaves,Mariana H.
The leishmaniases are severe parasitic diseases that occur worldwide, caused by protozoa of the genus Leishmania. Studies with medicinal plants can lead to a range of possibilities for treating and improving the patients' quality of life. Research on Azadirachta indica fractions and extracts has shown that they have excellent anti-leishmanial activity based on bioactivity-guided fractionation of ethanolic extracts of leaves and seeds and in vitro activity against promastigotes. In this research the most efficient extracts and fractions were selected for tests on intracellular amastigotes of Leishmania amazonensis. The ethanolic extract of the leaves and dichloromethane and chloroform fractions had IC50 values of 38, 3.9 and 1.2 &amp;#956;g/mL for...
Tipo: Journal article
Palavras-chave: Anti-leishmanial; Azadirachta indica; Cytotoxicity; Leishmaniasis; Leishmania amazonensis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200002

Phytochemical and toxicological investigations of crude methanolic extracts, subsequent fractions and crude saponins of Isodon rugosus
Provedor de dados: 48
Autores: Sadiq,Anwar Zeb Abdul; Ullah,Farhat; Ayaz,Sajjad Ahmad Muhammad.
BACKGROUND: Isodon rugosus is used traditionally in the management of hypertension, rheumatism, tooth-ache and pyrexia. Present study was arranged to investigate I. rugosus for phytoconstituents, phytotoxic and cytotoxic activities to explore its toxicological, pharmacological potentials and to rationalize its ethnomedicinal uses. Briefly, qualitative phytochemical analysis of the plant extracts were carried out for the existence of alkaloids, flavonoids, saponins, oils, glycosides, anthraquinones, terpenoids, sterols and tannins. Plant crude methanolic extract (Ir.Cr), its subsequent fractions; n-hexane (Ir.Hex), chloroform (Ir.Chf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq) and saponins (Ir.Sp) in different concentrations were tested for phytotoxic and...
Tipo: Journal article
Palavras-chave: Isodon rugosus; Cytotoxicity; Phytotoxicity; Brine shrimps.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100051

Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
Provedor de dados: 48
Autores: Soler,Maritza C; Molina,Jessica L; Díaz,Hugo A; Pinto,Vivian C; Barrios,Yasenka L; He,Kan; Roller,Marc; Weinstein-Oppenheimer,Caroline R.
Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine...
Tipo: Journal article
Palavras-chave: Cimicifuga foetida; Cytotoxicity; Hsp-27; Immunofluorescence; MCF-7 cells; RT-PCR.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005

Extraction of saponins and toxicological profile of Teucrium stocksianum boiss extracts collected from District Swat, Pakistan
Provedor de dados: 48
Autores: Shah,Syed Muhammad Mukarram; Sadiq,Abdul; Shah,Syed Muhammad Hassan; Khan,Shahzeb.
BACKGROUND: The current era is facing challenges in the management of neoplasia and weeds control. The currently available anti-cancer and herbicidal drugs are associated with some serious side effects. Therefore numerous researchers are trying to discover and develop plant based alternative particularly for the rational management of cancer and weed control. Teucrium stocksianum possess antioxidant and analgesic activities. The current study was designed to evaluate crude saponins (CS), methanolic extract and sub-fractions of T. stocksianum for cytotoxic and phytotoxic potentials. CS, methanolic extract and sub-fractions were extracted from powdered plant material using different solvents. Cytotoxic potential of the extracts at a dose of 10, 100 and 1000...
Tipo: Journal article
Palavras-chave: Saponins; Teucrium stocksianum; Cytotoxicity; Phytotoxicity.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100059

Induction of programmed cell death by Prangos uloptera, a medicinal plant
Provedor de dados: 48
Autores: ZAHRI,SABER; RAZAVI,SEYED MEHDI; NIRI,FARSHAD HASSANZADEH; MOHAMMADI,SARIEH.
Inhibition of the cell growth or induction of cell death is the most promising area in cancer therapy. The induction of apoptosis by dichloromethane extract of Prangos uloptera was evaluated on the McCoy cell line. This plant's roots, aerial parts and fruit have medicinal value. Cell growth inhibitory and cell cytotoxicity effects of the extract were assayed by MTT and Trypan-blue tests, respectively. Morphological changes and DNA fragmentation were also evaluated. The viability tests showed 0.49 and 0.3 mg/ml as 50% inhibition concentration and 50% cytotoxicity concentration after 24 hours of treatment, respectively. Fluorescent microscopy analysis revealed chromatin fragmentation and scanning electron microscopy showed cell shrinkage and cytoplasmic...
Tipo: Journal article
Palavras-chave: Apoptosis; Cytotoxicity; McCoy; Prangos uloptera.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400013

Cytotoxicity, nitric oxide and acetylcholinesterase inhibitory activity of three limonoids isolated from Trichilia welwitschii (Meliaceae)
Provedor de dados: 48
Autores: Dzoyem,Jean P.; Tsamo,Armelle T.; Melong,Raduis; Mkounga,Pierre; Nkengfack,Augustin E.; McGaw,Lyndy J.; Eloff,Jacobus N.
BACKGROUND: Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric oxide (NO) and acetylcholinesterase (AChE) inhibitory activity and the cytotoxicity of three limonoids: trichilia lactone D5 (1), rohituka 3 (2) and dregeanin DM4 (3), isolated from Trichilia welwitschii C.DC RESULTS: Results indicated that the three limonoids had low cytotoxicity towards Vero cells with LC50 values ranging from 89.17 to 75.82 (&amp;#956;g/L. Compounds (2) and (3) had lower cytotoxicity compared to puromycin and doxorubicin...
Tipo: Journal article
Palavras-chave: Trichilia welwitschii; Cytotoxicity; Acetylcholinesterase; Nitric oxide; Limonoids.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100057

Identification of a fat-soluble fraction of Sparganium stoloniferum that inhibits cervical cancer HeLa cells
Provedor de dados: 52
Autores: Zhang,Junwu; Wei,Yahui.
ABSTRACT  Context The rizoma of Sparganium stoloniferum has been used as a traditional Chinese medicinal herb for thousands of years. Sparganium stoloniferum is a stasis-breaking drug to treat a wide range of diseases including cancer,however, its activity of extract on cervical cancer HeLa cells and the mechanisms remains unknown.  Objective This study aimed to screen Sparganium stoloniferum extract for its inhibitory effects on cervical cancer HeLa cells.  Materials and methods Sparganium stoloniferum was extracted with 95% ethanol under reflux, and the extracts were preliminary separated by silica gel column chromatography. MTT assay and flow cytometry were used to determine the inhibitory effects of three fractions on HeLa cells. In addition, GC-MS was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sparganium stoloniferum; Fat-soluble active fraction; Cytotoxicity; HeLa cells.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100337

Cytotoxicity and genotoxicity of untreated hospital effluents
Provedor de dados: 52
Autores: Ortolan,Maria da Graça Silva; Ayub,Marco Antônio Záchia.
Untreated hospital effluent samples were tested for cytotoxic and genotoxicity in order to access whether they presented any serious health hazards. Results of umuC test suggested that the effluent from a large, general hospital could be considered weakly genotoxic. Comparatively, effluents from inpatient units presented higher frequency of genotoxicity than those coming from laboratory facilities. Although no cytotoxic activity was detected in laboratory effluent samples, cytotoxicity appeared to be an important problem of effluents from inpatient units.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Cytotoxicity; Untreated hospital effluents; Water sources contamination.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400009

Potential Use of Polysaccharides from the Brown Alga Undaria pinnatifida as Anticoagulants
Provedor de dados: 52
Autores: Faggio,Caterina; Morabito,Marina; Minicante,Simona Armeli; Piano,Giada Lo; Pagano,Maria; Genovese,Giuseppa.
Undaria pinnatifida (U. pinnatifida) is a highly invasive species and has caused concern all over the world because it has invaded coastal environments, has the potential to displace native species, significantly alters habitat for associated fauna, and disturbs navigation. Any attempt to eradicate it would be futile, owing to the elusive, microscopic gametophyte, and because the alga thrives in sites rich in anthropic activities. Venice Lagoon is the largest Mediterranean transitional environment and the spot of the highest introduction of non-indigenous species, including U. pinnatifida, which is removed as a waste. We demonstrated that polysaccharide extracts from U. pinnatifida have an anticoagulant effect on human blood in vitro and are not cytotoxic....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Algal extract; Anticoagulant activity; Cytotoxicity; Fucoidans; Human blood cells; Undaria pinnatifida.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500798

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology
Provedor de dados: 52
Autores: Turkez,Hasan; Sonmez,Erdal; Turkez,Ozlem; Mokhtar,Yousef Ibrahem; Stefano,Antonio Di; Turgut,Guven.
Tungsten (VI) oxide (WO3) nanoparticles (NPs) are used for many industrial purposes in everyday life. However, their effects on human health have not been sufficiently evaluated. Therefore, the present study was designed to investigate the toxicity potentials of various concentrations (0 to 1000 ppm) of WO3NPs (&lt;100 nm particle size) in cultured primary rat hepatocytes. The results of cell viability assay showed that the higher concentrations of dispersed WO3 NPs (300, 500 and 1000 ppm) caused significant (p&lt;0.05) decreases of cell viability. Also, dose dependent negative alterations were observed in oxidative status and antioxidant capacity levels after the application of WO3 in cultured rat primary hepatocytes. The results of genotoxicity tests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tungsten oxide; Nanotoxicity; Genotoxicity; Cytotoxicity; Hepatocytes; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400532

The investigation of cytogenetic and oxidative effects of diffractaic acid on human lymphocyte cultures
Provedor de dados: 52
Autores: Demir,Leyla; Toğar,Başak; Türkez,Hasan; Sozio,Piera; Aslan,Ali; Stefano,Antonio Di.
Diffractaic acid (DA) is a naturally occurring depside derivative found in several lichen species. It has a wide range of important biological effects such as analgesic and antiviral properties, although its cytotoxic, cytogenetic and oxidative effects have not been investigated in human blood tissue yet. Therefore, increasing concentrations (1, 5, 10, 25, 50, 100 and 200 mgL-1) of DA was added into human whole blood cultures. 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) assay was used to assess the cell viability and/or cytotoxicity and genotoxic damage potential of DA using chromosome aberration (CA) and micronucleus (MN) tests were performed. In addition, oxidative alterations were determined by the total antioxidant capacity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diffractaic acid; Cytotoxicity; Human blood; Genotoxicity; Oxidative status.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100075

In vitro cytotoxic, genotoxic and antioxidant/oxidant effects of guaiazulene on human lymphocytes
Provedor de dados: 52
Autores: Toğar,Başak; Çelik,Kübra; Türkez,Hasan.
The aim of this study was to evaluate for the cytotoxicity, genotoxicity and antioxidant/oxidant activity of GYZ on human peripheral blood lymphocytes (PBLs). Guaiazulene (GYZ) was added into culture tubes at various concentrations (0-400 µg/mL-1). Cytotoxicity against the human lymphocytes cultures was examined by lactate dehydrogenase (LDH) release assay. The proliferative response was estimated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Antioxidant/oxidant activity was evaluated by measuring the total oxidant status (TOS) and total antioxidant capacity (TAC) levels. Micronucleus (MN) and chromosomal aberration (CA) tests were used in genotoxicity studies. The results showed that GYZ caused cytotoxicity in the PBLs at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Guaiazulene; Cytotoxicity; Genotoxicity; Antioxidant activity; Oxidant status.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100061

Isolation, Characterization, Antioxidant, Antimicrobial and Cytotoxic Effect of Marine Actinomycete, Streptomyces Carpaticus MK-01, against Fish Pathogens
Provedor de dados: 52
Autores: Subramanian,Dharaneedharan; Kim,Min-Sun; Kim,Dong-Hwi; Heo,Moon-Soo.
ABSTRACT Present study aim to evaluate the antimicrobial, antioxidant and cytotoxic potential of crude extract of Marine Streptomyces carpaticus MK-01 isolated from seawater collected from Daejeong-cost of Jeju Island. About 24 actinomycetes strains were isolated and subjected to morphological and molecular analysis that confirmed the isolate as S. carpaticus MK-01. Crude ethyl acetate extract of MK-01 strain showed extensive antibacterial activity against Gram-positive fish pathogenic bacteria namely Streptococcus iniae and S. parauberis with a maximum zone of inhibition (0.92±0.03mm) was recorded against S. parauberis at the minimum extract concentration (3.12µg/ml). The MK-01 ethyl acetate extract shows dose dependant significant increase in antioxidant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinomycete; Streptomyces carpaticus MK-01; Antioxidant; Cytotoxicity; Jeju Island.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100437

Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
Provedor de dados: 52
Autores: Barreto,George Emílio Sampaio; Oliveira,Elineusa Silva de; Villar,Flávio Augusto Guerreiro Aragão de; Santos,Gleide Souza dos; Lima,Rute Maria Ferreira; Costa,Silvia Lima; Costa,Maria de Fátima Dias; Egito,Eryvaldo Sócrates Tabosa; El-Bachá,Ramon Santos.
The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astrocytes; Brain; Cytotoxicity; 3-methylcatechol; Peroxidation; Respiration.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500012

Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues
Provedor de dados: 52
Autores: Linan,Lamia Zuniga; Lima,Nadson Murilo Nascimento; Benatti,Carol; Xavier,Mariana; Rodrigues,Ana A.; Manenti,Flavio; Jardini,Andre; Maciel Filho,Rubens; Gilioli,Rovilson.
ABSTRACT A cytotoxicity study is performed on a poly(methyl methacrylate) polymer (PMMA) to be used for the fabrication of bone tissue by Rapid Prototyping (RP). The solution polymerization is conducted in a pilot plant reactor using more appropriated reagents in consideration of the medical application. Moreover, the polymer is efficiently handled to avoid the side effect of the monomer, reducing the concentration of this specie to 287,731 µg MMA/kg PMMA. The cytotoxicity of the polymer is determined through growth monitoring, adherence and morphology of L-929 cells. Additionally, MTT and LIVE/DEAD tests are performed. The results showed continuous and progressive growth of the cells on the surface of the specimens. Moreover, the material did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PMMA; Synthesis; Cytotoxicity; Viability; Mesenchymal cells.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100601

Antimicrobial, Antioxidant and Cytotoxic Activity of MarineStreptomyces parvulus VITJS11 Crude Extract
Provedor de dados: 52
Autores: Naine,S. Jemimah; Devi,C. Subathra; Mohanasrinivasan,V.; B,Vaishnavi.
The main aim of the study was to evaluate the bioactive properties of ethyl acetate crude extract of Streptomyces parvulus VITJS11 with a view to assess their therapeutic potential. The biological activity of ethyl acetate extract was tested against fungal and bacterial pathogens. The free radical scavenging potential of the crude extract was determined by DPPH assay. The chemo preventive properties of S. parvulus VITJS11 ethyl acetate extract was examined by MTT assay on HepG2 cells. The morphological, physiological and the biochemical properties of the strain S. parvulus VITJS11 was confirmed by conventional methods. Genotypic characterization was done using 16S r-DNA partial gene amplification and sequencing. The authenticity of the crude chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine actinomycetes; Streptomyces parvulusVITJS11; Antimicrobial activity; Antioxidants; Cytotoxicity; Bioactive compounds.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200198

Cytotoxic Effect on Cancerous Cell Lines by Biologically Synthesized Silver Nanoparticles
Provedor de dados: 52
Autores: Kulandaivelu,Balaji; Gothandam,K M.
The biosynthesis of nanoparticles has been proposed as an environmental friendly and cost effective alternative to chemical and physical methods. Silver nanoparticles are biologically synthesized and characterized were used in the study. The invitro cytotoxic effect of biologically synthesized silver nanoparticles against MCF-7 cancer cell lines were assessed. The cytotoxic effects of the silver nanoparticles could significantly inhibited MCF-7 cancer cell lines proliferation in a time and concentration-dependent manner by MTT assay. Acridine orange, ethidium bromide (AO/EB) dual staining, caspase-3 and DNA fragmentation assays were carried out using various concentrations of silver nanoparticles ranging from 1 to 100 μg/mL. At 100 μg/mL concentration, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver nanoparticle; Nanotechnology; Nanobiotechnology; Nanoparticle; Cytotoxicity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100307

Evaluation of cytotoxicity and biological activities in extracellular polysaccharides released by cyanobacterium Arthrospira platensis
Provedor de dados: 52
Autores: Challouf,Rafika; Trabelsi,Lamia; Ben Dhieb,Rym; El Abed,Omeya; Yahia,Ali; Ghozzi,Khamissa; Ben Ammar,Jihen; Omran,Hela; Ben Ouada,Hatem.
The aim of this work was to evaluate the cytotoxicity of Arthrospira platensis Extracellular Polymeric Substances (EPS) for colon cancer and kidney cell lines. Results showed that EPS were free from cytotoxic effects. A variety of solvents were assessed for their ability to extract the bioactive ingredients from EPS. Methanol gave the highest yield (75.75%) than other solvents. The extracts were tested for activities against a collection of Gram+/- bacteria. The methanol extract exhibited a more potent activity than the other organic extracts, whereas the aqueous extract was active against Staphylococcus epidermis (Gram+) and Salmonella typhimurium (Gram-). Finally, The extracts were also tested for the antioxidant activity, using the Trolox Equivalent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyanobacteria; Exoplysaccharides; Cytotoxicity; Antibacterial; Antioxidant; Activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400024

Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells
Provedor de dados: 56
Autores: Ribeiro-Dias,F.; Russo,M.; Nascimento,F.R.F.; Barbuto,J.A.M.; Timenetsky,J.; Jancar,S.
Macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elicited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited macrophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Macrophages; Cytotoxicity; Mycoplasma; Tumor cells; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100010

Cytotoxicity and genotoxicity of low doses of mercury chloride and methylmercury chloride on human lymphocytes in vitro
Provedor de dados: 56
Autores: Silva-Pereira,L.C.; Cardoso,P.C.S.; Leite,D.S.; Bahia,M.O.; Bastos,W.R.; Smith,M.A.C.; Burbano,R.R.
Mercury is a xenobiotic metal that is a highly deleterious environmental pollutant. The biotransformation of mercury chloride (HgCl2) into methylmercury chloride (CH3HgCl) in aquatic environments is well-known and humans are exposed by consumption of contaminated fish, shellfish and algae. The objective of the present study was to determine the changes induced in vitro by two mercury compounds (HgCl2 and CH3HgCl) in cultured human lymphocytes. Short-term human leukocyte cultures from 10 healthy donors (5 females and 5 males) were set-up by adding drops of whole blood in complete medium. Cultures were separately and simultaneously treated with low doses (0.1 to 1000 µg/l) of HgCl2 and CH3HgCl and incubated at 37ºC for 48 h. Genotoxicity was assessed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylmercury; Cytotoxicity; Genotoxicity; Mitotic index; Human lymphocytes; Chromosome aberrations.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600012

In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
Provedor de dados: 56
Autores: Isa,N.M.; Abdelwahab,S.I.; Mohan,S.; Abdul,A.B.; Sukari,M.A.; Taha,M.M.E.; Syam,S.; Narrima,P.; Cheah,S.Ch.; Ahmad,S.; Mustafa,M.R.
The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 µg/mL. The toxic effect of BA on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Boesenbergia rotunda; Boesenbergin A; Cytotoxicity; Antioxidation; Anti-inflammatory.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600008

Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells
Provedor de dados: 56
Autores: Rolim,P.M.; Fidelis,G.P.; Padilha,C.E.A.; Santos,E.S.; Rocha,H.A.O.; Macedo,G.R.
Melon (Cucumis melo L.) has high economic value and in recent years, its production has increased; however, part of the fruit is wasted. Usually, inedible parts such as peel and seeds are discarded during processing and consumption. Extracts of melon residues were prepared and their phenolic compounds, antioxidants and antiproliferative activities were evaluated. Total phenolic compounds were found in hydroethanolic, hydromethanolic, and aqueous extracts, especially for melon peel (1.016 mg gallic acid equivalent/100 g). Flavonoids total content found for melon peel aqueous extract was 262 µg of catechin equivalent (CA)/100 g. In all extracts of melon peel significant amounts of gallic acid, catechin, and eugenol were found. For total antioxidant capacity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melon residues; Antioxidant activity; Cancer cells; Free radical scavenging activity; Metal chelator; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400607

Trypan blue exclusion assay by flow cytometry
Provedor de dados: 56
Autores: Avelar-Freitas,B.A.; Almeida,V.G.; Pinto,M.C.X.; Mourão,F.A.G.; Massensini,A.R.; Martins-Filho,O.A.; Rocha-Vieira,E.; Brito-Melo,G.E.A.
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypan blue; Fluorescence; Flow cytometry; Cytotoxicity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307

Cytotoxicity of chlorhexidine digluconate to murine macrophages and its effect on hydrogen peroxide and nitric oxide induction
Provedor de dados: 56
Autores: Bonacorsi,C.; Raddi,M.S.G.; Carlos,I.Z.
Chlorhexidine, even at low concentrations, is toxic for a variety of eukaryotic cells; however, its effects on host immune cells are not well known. We evaluated in vitro chlorhexidine-induced cytotoxicity and its effects on reactive oxygen/nitrogen intermediate induction by murine peritoneal macrophages. Thioglycollate-induced cells were obtained from Swiss mice by peritoneal lavage with 5 ml of 10 mM phosphate-buffered saline, washed twice and resuspended (10(6) cells/ml) in appropriate medium for each test. Cell preparations contained more than 95% macrophages. The cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the presence of hydrogen peroxide (H2O2) and nitric oxide (NO) by the horseradish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorhexidine; Cytotoxicity; Macrophages; Nitric oxide; Hydrogen peroxide.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200007

In vitro cytotoxicity of the LDE: daunorubicin complex in acute myelogenous leukemia blast cells
Provedor de dados: 56
Autores: Dorlhiac-Llacer,P.E.; Marquezini,M.V.; Toffoletto,O.; Carneiro,R.C.G.; Maranhão,R.C.; Chamone,D.A.F.
Acute myelogenous leukemia (AML) blast cells show high-affinity degradation of low-density lipoprotein (LDL), suggesting an increased expression of cellular LDL receptors. LDE is a lipid microemulsion easily synthesized in vitro which is known to mimic the metabolic pathway of LDL. We used LDE as a carrier for daunorubicin and assayed the cytotoxicity of the complex using AML blast cells since RT-PCR analysis showed that AML cells express LDL receptor mRNA. The LDE:daunorubicin complex killed 46.7% of blast cells and 20.2% of normal bone marrow cells (P&lt;0.001; Student t-test). Moreover, this complex destroyed AML blast cells as efficiently as free daunorubicin. Thus, LDE might be a suitable carrier of chemotherapeutic agents targeting these drugs to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Acute myelogenous leukemia; Daunorubicin; Low-density lipoprotein; LDE complex; RT-PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000004

Two new mixed-ligand coordination polymers based on multi-N chelating ligand inhibit YAP expression and induce caspase-mediated spinal tumor cell apoptosis
Provedor de dados: 56
Autores: Sun,Yi Fu; Shao,Li Wei; Chen,Qi; Gao,Xu; Li,Fang; Wu,Chang Yan.
Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6′-bis-(1H-benzoimidazol-2-yl)-2,2′-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2 as the foundational building block. In addition, the Cell Counting Kit-8 assay was conducted to evaluate the anti-proliferation activity of compounds 1 and 2 against human spinal tumor cells OPM-2. The cell viability curves showed that the two compounds have anti-proliferation activity on spinal tumor cells, and the activity of compound 1 is higher than compound 2. The annexin V-FITC/PI assay and western...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coordination polymers; Glioma cell strains; Cytotoxicity; Apoptosis; Immunoblotting.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500608

Role of nitric oxide and superoxide in Giardia lamblia killing
Provedor de dados: 56
Giardia lamblia trophozoites were incubated for 2 h with activated murine macrophages, nitric oxide (NO) donors or a superoxide anion generator (20 mU/ml xanthine oxidase plus 1 mM xanthine). Activated macrophages were cytotoxic to Giardia trophozoites (~60% dead trophozoites). This effect was inhibited (&amp;gt;90%) by an NO synthase inhibitor (200 µM) and unaffected by superoxide dismutase (SOD, 300 U/ml). Giardia trophozoites were killed by the NO donors, S-nitroso-acetyl-penicillamine (SNAP) and sodium nitroprusside (SNP) in a dose-dependent manner (LD50 300 and 50 µM, respectively). A dual NO-superoxide anion donor, 3-morpholino-sydnonimine hydrochloride (SIN-1), did not have a killing effect in concentrations up to 1 mM. However, when SOD (300 U/ml)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Macrophage; Peroxynitrite; Giardia lamblia; Cytotoxicity; Superoxide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100015

Dipyridamole increases the cytotoxicity of cisplatin in human larynx cancer cells in vitro
Provedor de dados: 56
Autores: Rodrigues,M.; Barbosa Junior,F.; Perussi,J.R.
This paper describes the effect of dipyridamole (DIP) on the cytotoxicity of cisplatin in HEp-2 human larynx cancer cells in vitro and the nature of the interaction between cisplatin and dipyridamole. Cytotoxic assays were performed to obtain the IC50 for cisplatin. The cells were treated with 0, 20, 40, 80, 120 or 200 µM cisplatin, with or without a single concentration of DIP and incubated for 60 min at 37ºC and 5% CO2 for 3 days and then counted with a hemocytometer. The accumulation of cisplatin in the cells was measured by atomic absorption and fluorescence was used to determine the membrane binding constant of DIP. In the presence of 10, 20 and 30 µM DIP, the IC50 of cisplatin was reduced by 25, 60 and 82% in HEp-2 cells. Combination index analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Dipyridamole; Synergism; Tumor cells; Cytotoxicity; IC50.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400017

The aminoglycoside antibiotic gentamicin is able to alter metabolic activity and morphology of MDCK-C11 cells: a cell model of intercalated cells
Provedor de dados: 56
Autores: Coutinho,A.G.G.; Biscaia,S.M.P.; Fernandez,R.; Tararthuch,A.L.
It is well known that the aminoglycoside antibiotic gentamicin is capable of causing damage to kidney cells. Given the known involvement of Ca2+ in the nephrotoxic action of gentamicin, the purpose of this study was to establish a relationship between the concentration of intracellular Ca2+ ([Ca2+]i) and cellular cytotoxicity using MDCK-C11 cells, a clone that has several properties that resemble those of intercalated cells of the distal nephron. Changes in [Ca2+]i was determined using fluorescence microscopy. Cell viability was evaluated by the neutral red method, and cell cytotoxicity by the MTT method. The [Ca2+]i gradually increased when cells were exposed to 0.1 mM gentamicin for 10, 20, and 30 min. The presence of extracellular Ca2+ was found to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gentamicin; [Ca2+]i; MDCK-C11 cells; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000610

Cytotoxic effect of essential oil of thyme (Thymus broussonettii) on the IGR-OV1 tumor cells resistant to chemotherapy
Provedor de dados: 56
Autores: Ait M'Barek,L.; Ait Mouse,H.; Jaâfari,A.; Aboufatima,R.; Benharref,A.; Kamal,M; Bénard,J.; El Abbadi,N.; Bensalah,M.; Gamouh,A.; Chait,A.; Dalal,A.; Zyad,A.
The anti-tumor effect of the Moroccan endemic thyme (Thymus broussonettii) essential oil (EOT) was investigated in vitro using the human ovarian adenocarcinoma IGR-OV1 parental cell line OV1/P and its chemoresistant counterparts OV1/adriamycin (OV1/ADR), OV1/vincristine (OV1/VCR), and OV1/cisplatin (OV1/CDDP). All of these cell lines elicited various degrees of sensitivity to the cytotoxic effect of EOT. The IC50 values (mean ± SEM, v/v) were 0.40 ± 0.02, 0.39 ± 0.02, 0.94 ± 0.05, and 0.65 ± 0.03% for OV1/P, OV1/ADR, OV1/VCR, and OV1/CDDP, respectively. Using the DBA-2/P815 (H2d) mouse model, tumors were developed by subcutaneous grafting of tumor fragments of similar size obtained from P815 (murin mastocytoma cell line) injected in donor mouse....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oil; Thymus broussonettii; Ovarian cancer; Cytotoxicity; OV1/adriamycin; OV1/vincristine; OV1/cisplatin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100014

The cytotoxicity of methacryloxylethyl cetyl ammonium chloride, a cationic antibacterial monomer, is related to oxidative stress and the intrinsic mitochondrial apoptotic pathway
Provedor de dados: 56
Autores: Sai,Ma; Le-qun,Shan; Yu-hong,Xiao; Fang,Li; Li,Huang; Shen,Lijuan; Ji-hua,Chen.
Antibacterial monomers incorporated in dentin bonding systems may have toxic effects on the pulp. Thus, the cytotoxicity of antibacterial monomers and its underlying mechanisms must be elucidated to improve the safety of antibacterial monomer application. The influence of an antibacterial monomer, methacryloxylethyl cetyl ammonium chloride (DMAE-CB), on the vitality of L929 mouse fibroblasts was tested using MTT assay. Cell cycle progression was studied using flow cytometry. Production of intracellular reactive oxygen species (ROS) after DMAE-CB treatment was measured using 2,7-dichlorodihydrofluorescein diacetate staining and flow cytometry analysis. Loss of mitochondrial membrane potential, disturbance of Bcl-2 and Bax expression, as well as release of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quaternary ammonium compounds; Dental monomer; Cytotoxicity; Apoptosis; Reactive oxygen species; Methacryloxylethyl cetyl ammonium chloride.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100008

Anti-tumor properties of blackseed (Nigella sativa L.) extracts
Provedor de dados: 56
Autores: Ait Mbarek,L.; Ait Mouse,H.; Elabbadi,N.; Bensalah,M.; Gamouh,A.; Aboufatima,R.; Benharref,A.; Chait,A.; Kamal,M.; Dalal,A.; Zyad,A.
The objective of the present study was to evaluate the in vitro and in vivo anti-cancer effect of Nigella sativa L. seed extracts. The essential oil (IC50 = 0.6%, v/v) and ethyl acetate (IC50 = 0.75%) extracts were more cytotoxic against the P815 cell line than the butanol extract (IC50 = 2%). Similar results were obtained with the Vero cell line. Although all extracts had a comparable cytotoxic effect against the ICO1 cell line, with IC50 values ranging from 0.2 to 0.26% (v/v), tests on the BSR cell line revealed a high cytotoxic effect of the ethyl acetate extract (IC50 = 0.2%) compared to the essential oil (IC50 = 1.2%). These data show that the cytotoxicity of each extract depends on the tumor cell type. In vivo, using the DBA2/P815 (H2d) mouse model,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nigella sativa L.; Extracts; Cytotoxicity; Anti-tumor activity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600014

Cytotoxic and DNA-topoisomerase effects of lapachol amine derivatives and interactions with DNA
Provedor de dados: 56
Autores: Esteves-Souza,A.; Figueiredo,D.V.; Esteves,A.; Câmara,C.A.; Vargas,M.D.; Pinto,A.C.; Echevarria,A.
The cytotoxic activity of amino (3a-e), aza-1-antraquinone (4a-e) lapachol derivatives against Ehrlich carcinoma and human K562 leukemia cells was investigated. Cell viability was determined using MTT assay, after 48 (Ehrlich) or 96 h (K562) of culture, and vincristine (for K562 leukemia) and quercetin (for Ehrlich carcinoma) were used as positive controls. The results showed dose-dependent growth-inhibiting activities and that the amino derivatives were active against the assayed cells, whereas the 4a-e derivatives were not. The allylamine derivative 3a was the most active against Ehrlich carcinoma, with IC50 = 16.94 ± 1.25 µM, and against K562 leukemia, with IC50 = 14.11 ± 1.39 µM. The analogous lawsone derivative, 5a, was also active against Ehrlich...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lapachol; Amino-lapachol derivatives; Cytotoxicity; DNA-topoisomerase; DNA-interactions.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000013

Preparation and cytotoxicity of cisplatin-containing liposomes
Provedor de dados: 56
Autores: Carvalho Júnior,A.D.; Vieira,F.P.; De Melo,V.J.; Lopes,M.T.P.; Silveira,J.N.; Ramaldes,G.A.; Garnier-Suillerot,A.; Pereira-Maia,E.C.; De Oliveira,M.C.
We encapsulated cisplatin into stealth pH-sensitive liposomes and studied their stability, cytotoxicity and accumulation in a human small-cell lung carcinoma cell line (GLC4) and its resistant subline (GLC4/CDDP). Since reduced cellular drug accumulation has been shown to be the main mechanism responsible for resistance in the GLC4/CDDP subline, we evaluated the ability of this new delivery system to improve cellular uptake. The liposomes were composed of dioleoylphosphatidylethanolamine (DOPE), cholesteryl hemisuccinate (CHEMS), and distearoylphosphatidylethanolamine-polyethyleneglycol 2000 (DSPE-PEG2000) and were characterized by determining the encapsulation percentage as a function of lipid concentration. Among the different formulations,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Liposomes; Encapsulation; Cytotoxicity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800017

Cytotoxic activity of BCG-activated macrophages against L929 tumor cells is nitric oxide-dependent
Provedor de dados: 56
Autores: Nascimento,F.R.F.; Ribeiro-Dias,F.; Russo,M.
The tumoricidal activity of activated macrophages has been attributed largely to the release of tumor necrosis factor (TNF), or to the production of reactive oxygen or nitrogen intermediates. The L929 tumor cell line (a murine fibroblast-like cell) when treated with actinomycin D (ActD) has been used to measure TNF&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt; cytotoxicity. In the present study, we determined the cytotoxic activity of BCG-activated peritoneal macrophages against ActD-untreated L929 tumor cells. Furthermore, we measured the production of hydrogen peroxide (H2O2), nitric oxide (NO) and TNF by macrophages cultured in the presence or absence of L929 cells. As expected, BCG-activated macrophages produced significant amounts of H2O2 (16.0 ± 3.0 µM),...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytotoxicity; Macrophages; L929 tumor cells; Nitric oxide; TNF; Hydrogen peroxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200012

Kinetic properties of Streptomyces canarius L- Glutaminase and its anticancer efficiency
Provedor de dados: 58
Autores: Reda,Fifi M.
Abstract L-glutaminase was produced by Streptomyces canarius FR (KC460654) with an apparent molecular mass of 44 kDa. It has 17.9 purification fold with a final specific activity 132.2 U/mg proteins and 28% yield recovery. The purified L-glutaminase showed a maximal activity against L-glutamine when incubated at pH 8.0 at 40 °C for 30 min. It maintained its stability at wide range of pH from 5.0 11.0 and thermal stable up to 60 °C with Tm value 57.5 °C. It has high affinity and catalytic activity for L-glutamine (Km 0.129 mM, Vmax 2.02 U/mg/min), followed by L-asparagine and L-aspartic acid. In vivo, L-glutaminase showed no observed changes in liver; kidney functions; hematological parameters and slight effect on RBCs and level of platelets after 10 days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-glutaminase; Anti-cancer; Cytotoxicity; MTT assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400957

Virulence and cytotoxicity of seafood borne Aeromonas hydrophila
Provedor de dados: 58
Autores: Illanchezian,Seethalakshmi; Jayaraman,SathishKumar; Manoharan,Muthu Saravanan; Valsalam,Saritha.
The present study was conducted to determine the virulence and cytotoxicity of Aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in Chennai, Tamil Nadu, India. Among 73 A. hydrophila strains isolated from fish and shrimp samples, 86.3% exhibited haemolysis, 78.1% produced slime, 98.63% produced protease and also demonstrated cytotoxicity on Vero cells. Cell shrinkage, detachment and rounding of Vero cells were recorded as cytotoxic changes. Only one strain did not show haemolysis, slime production, proteolytic activity and cytotoxicity on treatment with Vero cells. Positive correlation was observed between proteolytic activity and cytotoxicity irrespective of haemolytic activity of the strains. These results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas hydrophila; Seafood; Virulence; Cytotoxicity; Vero cells.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400016

Cytotoxicity of subfractions and compounds from Polymnia sonchifolia
Provedor de dados: 58
Autores: Fernandes,Maria Judite B.; Limas,Caroline; Rossi,Maria Helena; Gonçales,Edlayne; Simoni,Isabela C.
Our previous works showed that extracts and fractions from Polymnia sonchifolia had presented an inhibitory activity on growth and aflatoxin production by Aspergillus flavus in non-cytotoxic concentrations. In this work, report results on the in vitro cytotoxicity of subfractions and of a mixture of sesquiterpenes lactones from Polymnia sonchifolia on Vero cells. The cytotoxicity was assayed through crystal violet staining (CVS) method and the 50% inhibitory concentration for cell growth (IC50) was calculated. Both subfractions and the mixture of sesquiterpenes lactones did not show in vitro cytotoxicity to Vero cells on active biologically concentrations against production of aflatoxin B1 by Aspergillus flavus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymnia sonchifolia; Subfractions; Lactones; Cytotoxicity; Crystal violet staining (CVS) method.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400006

Activity of the aqueous extract from Polymnia sonchifolia leaves on growth and production of aflatoxin B1 by Aspergillus flavus
Provedor de dados: 58
Autores: Pinto,Marina M.; Gonçalez,Edlayne; Rossi,Maria H.; Felício,Joana D.; Medina,Cláudia S.; Fernandes,Maria J.B.; Simoni,Isabela C.
The aqueous extract from Polymnia sonchifolia leaves (AE) was tested for inhibitory activity on aflatoxin B1(AFB1) production and growth of Aspergillus flavus. The cytotoxicity of AE on Vero cells was also performed. Suspensions of A. flavus spores were inoculated into 50 mL of YES medium together with different concentrations of the AE. The aflatoxin B1 was extracted, analyzed by thin layer chromatography and quantified by photodensitometry. All the concentrations of AE induced inhibition of AFB1 production. The aqueous extract showed in vitro cytotoxicity to Vero cells only at concentrations above 500 µg/mL.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxin B1; Aspergillus flavus; Polymnia sonchifolia; Cytotoxicity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200011

Cytotoxic and apoptotic potential of Myristica fragrans Houtt. (mace) extract on human oral epidermal carcinoma KB cell lines
Provedor de dados: 60
Autores: Rengasamy,Gayathri; Venkataraman,Anuradha; Veeraraghavan,Vishnu Priya; Jainu,Mallika.
Several studies have revealed that certain naturally occurring medicinal plants inhibit the growth of various cancers. The present study was conducted to evaluate cytotoxicity and apoptotic induction potential of Myristica fragrans Houtt mace extract. The cytotoxic activity of the Myristica fragrans Houtt mace acetone extract was assayed by MTT assay on human oral epidermal carcinoma KB cell lines. KB cells were incubated with different concentration of mace extract ranging from 25 to 125 μg/mL for 24hrs. The apoptotic induction potential was also studied by the analysis of Bcl-2 protein and gene expression in mace extract incubated KB cell lines using western blotting technique and real-time polymerase chain reaction. The mace extract exhibited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myristica fragrans Houtt; (mace)/extract; Apoptosis; Cytotoxicity; KB cell lines; MTT assay; Bcl-2 gene.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300629

In vitro anticancer activity of microbial isolates from diverse habitats
Provedor de dados: 60
Autores: Thomas,Angel Treasa; Rao,Josyula Venkata; Subrahmanyam,Volety Mallikarjuna; Chandrashekhar,Hariharapura Raghu; Maliyakkal,Naseer; Kisan,Tukaram Kedar; Joseph,Alex; Udupa,Nayanabhirama.
Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticancer; Cytotoxicity; Flow cytometry; Microbial extracts; MTT assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200009

Development of nanotechnology-based drug delivery systems with olive vegetable oil for cutaneous application
Provedor de dados: 60
Autores: Silva,Silas Arandas Monteiro e; Lacerda,Rísia; Bernegossi,Jéssica; Chorilli,Marlus; Leonardi,Gislaine Ricci.
ABSTRACT Liquid-Crystalline Systems represent active compounds delivery systems that may be able to overcome the physical barrier of the skin, especially represented by the stratum corneum. To obtain these systems, aqueous and oily components are used with surfactants. Of the different association structures in such systems, the liquid-crystalline offer numerous advantages to a topical product. This manuscript presents the development of liquid-crystalline systems consisting, in which the oil component is olive oil, its rheological characterizations, and the location of liquid crystals in its phase map. Cytotoxic effects were evaluated using J-774 mouse macrophages as the cellular model. A phase diagram to mix three components with different proportions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liquid-Crystalline Systems/development; Liquid-Crystalline Systems/reology; Drugs/skin delivery; Olive Oil/use/Liquid-Crystalline Systems; Rheology; Cytotoxicity; Bioadhesion.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100023

Laurus nobilis L.: assessment of the cytotoxic and genotoxic potential of aqueous extracts by micronucleus and Allium cepa assays
Provedor de dados: 60
Autores: Silva,Mayara Christine; Matos,Aline Ferreira; Santos,Hataânderson Luíz Cabral dos; Gomes,Jennifer Vieira; Pastura,Danilo Giovanni Narciso; Pereira,Gabriela Lopes; Rocha,Ellen Borges da; Larangeira,Mayara de Jesus Costa; Alves,Raphael Santiago; Bastos,Leonardo de Oliveira; Borba,Helcio Resende; Lima,Viviane Moreira de.
Laurus nobilis L. is a large shrub belonging to the Lauracea family. Its leaves are widely used for food seasoning as well as in folk medicine. Various studies have demonstrated the antiproliferative, antifungal and antibacterial effects of Laurus nobilis, but no studies have investigated the genotoxic effect of the aqueous extract of the plant. The objective of this study was to analyze the genotoxic potential of an aqueous extract of leaves, using the Allium cepa assay and mouse peripheral blood cell micronucleus test. The results showed that the extract did not have any genotoxic activity, but cytotoxic activity was observed in the two experimental models used. The extract had an antiproliferative effect, detected through the reduction of the mitotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laurus nobilis; Allium cepa; Micronucleus; Genotoxicity; Cytotoxicity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100534

4-Nerolidylcatechol induces autophagy in human glioblastoma cells
Provedor de dados: 60
Autores: Massaro,Renato Ramos; Brohem,Carla Abdo; Almeida,Rebeca Leite de; Rivelli,Diogo Pineda; Miyake,Juliano Andreoli; Colquhoun,Alison; Barros,Silvia Berlanga de Moraes; Maria-Engler,Silvya Stuchi.
ABSTRACT Gliomas account for the majority of primary malignant brain tumors and present invasive behavior into adjacent healthy tissue. While 4-NC had previously shown to induce apoptotic cell death in a melanoma model, for the glioma model described in this paper 4-NC is cytotoxic for the cells with the induction of the autophagic pathway. Trypan blue exclusion assay showed that 4-NC was cytotoxic in a dose-dependent manner for A172 and T98G cell lines. IC10 and IC50 values were at 32 µM and 41 µM for A172 and T98G respectively. Inhibition of cell proliferation was observed by total cell counts and by cell cycle analysis by flow cytometry, with cell cycle arrest of A172 and T98G cell lines respectively in the G1/G0 and S phases of the cell cycle. 4-NC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-Nerolidylcatechol; Glioblastoma; Cytotoxicity; Autophagy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300618

Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest)
Provedor de dados: 60
Autores: Vilar,Juliana Brandstetter; Andrade,Laryssa Silva de; Leite,Kaio Ramos; Ferreira,Heleno Dias; Chen,Lee Chen.
Curatella americana L., commonly known as "lixeira" in Brazil, has been used in folk medicine to treat ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic potential of the ethanolic extract of C. americana stem bark using the prophage λ induction test (SOS inductest). To evaluate the cytotoxicity of this plant, after treatment with different concentrations of the extract, Escherichia coli WP2s(λ) cultures were diluted in M9 buffer, inoculated into LB plates, and incubated for 24 h at 37 °C. To assess genotoxicity, the lysogenic strain E. coli WP2s(λ) was treated with different concentrations of the extract. Then, the lysogenic strain was added to the indicator strain (RJF013), LB(1/2)(malt/amp), seeded into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curatella americana; Lixeira; Cytotoxicity; Genotoxicity; Lysogenic induction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300015

Cytotoxicity and antioxidant activity of goldenberry extracts obtained with high intensity ultrasound
Provedor de dados: 65
Autores: Codevilla,Cristiane Franco; Tischer,Bruna; Gindri,Amanda Leitão; Nogueira-Librelotto,Daniele Rubert; Barin,Juliano Smanioto; Silva,Cristiane de Bona da; Rolim,Clarice Madalena Bueno; Zepka,Leila Queiroz; Menezes,Cristiano Ragagnin de.
ABSTRACT: The high intensity ultrasound-assisted extraction (HIU) is one of the most simple, quick and efficient techniques for the extraction of phenolic and other antioxidant compounds from plants. This is the first application of HIU for the extraction of these compounds from goldenberry fruit. The HIU and conventional extraction techniques showed similar results regarding to phenolic compounds and antioxidant capacity. However, the time required for HIU extraction (5min) was 24 times lower than conventional extraction (120min). Phenolic compounds reported were chlorogenic acid, caffeic acid and rutin. In vitro cytotoxicity assays were used for evaluation of extracts and the results showed that in a wide range of concentration, the extract maintains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goldenberry; Sonication; Antioxidant capacity; Cytotoxicity; High performance liquid chromatography.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200752