Consulta: subjectFacets:"DNA isolation"
Registros recuperados: 5
Data/hora: 10/06/2024 11:06:01

CTAB methods for DNA extraction of sweetpotato for microsatellite analysis
Provedor de dados: Scientia Agricola
Autores: Borges,Aline; Rosa,Mariana Silva; Recchia,Gustavo Henrique; Queiroz-Silva,Jurema Rosa de; Bressan,Eduardo de Andrade; Veasey,Elizabeth Ann.
Microsatellite markers have proved to be useful in genetic diversity assessments of sweetpotato (Ipomoea batatas) but practical DNA extraction methods to ensure good quality and quantity DNA for these studies are yet to be established. This study compares the efficiency of three modified methodologies for DNA extraction of six sweetpotato landraces using the CTAB extraction buffer in regard to quantity and purity of DNA quantification and microsatellite band patterns. All methodologies yielded satisfactory results, but the method based in leaf tissue macerated in liquid nitrogen was deemed more adequate because of its simplicity and lower cost. However, the method based in dry leaf tissue was considered more advantageous, first because elicits...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ipomoea batatas; SSR; DNA isolation; Landraces; Protocol.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000400015

Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development
Provedor de dados: Acta Agronómica
Autores: Henao Ramírez,Ana María; Salazar Duque,Héctor Jaime; Urrea Trujillo,Aura Inés.
Abstract Theobroma cacao L. and its products are consumed worldwide. Those products are of great research interest due to antioxidant properties of some of their polyphenolic constituents. The amount of these polyphenols and polysaccharides has shown that can interfere with the high quality and quantity of nucleic acids for molecular research. Therefore, cocoa DNA extraction protocols can require a large amount of plant material and optimization time according with plant material source. The aim of this study was to evaluate the quality and quantity of DNA isolated from field plant leaves at different developmental stages from TSH565 genotype using different DNA extraction protocols. In addition, DNA extraction protocol was evaluated for small amount of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA isolation; Field cocoa plants; In vitro cocoa plantlets; Leaf development; PCR; SSRs.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200311

A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment
Provedor de dados: Acta Amazonica
Autores: MANGARAVITE,Érica; TERRA,Vanessa; HATTORI,Eric Koiti Okiyama; DAL’SASSO,Thaís Carolina da Silva; BHERING,Leonardo Lopes; OLIVEIRA,Luiz Orlando de.
ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Ascorbic acid; Dithiothreitol; DNA isolation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400335

An alternative method for Staphylococcus aureus DNA isolation
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Chapaval,L.; Moon,D.H.; Gomes,J.E.; Duarte,F.R.; Tsai,S.M.
This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Genomic DNA; DNA isolation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004

Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Moreira,Mônica; Noschang,Juliana; Neiva,Ivana Froede; Carvalho,Yanê; Higuti,llma Hiroko; Vicente,Vânia Aparecida.
In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus; DNA isolation; CTAB; Ultrasound.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013