Consulta: subjectFacets:"DNA methylation"
Registros recuperados: 28
Data/hora: 11/06/2024 00:32:48

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker
Provedor de dados: Electronic Journal of Biotechnology
Autores: Tiwari,Jagesh Kumar; Saurabh,Shaumaya; Chandel,Poonam; Singh,Bir Pal; Bhardwaj,Vinay.
Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum. Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) were applied to detect the genetic and epigenetic changes, respectively in the somatic hybrids mother plants (1st cycle) and their regenerants (30th cycles sub-cultured). Results: To detect genetic changes, eight AFLP primer combinations yielded a total of 329 scorable bands of which 49 bands were polymorphic in both mother plants and regenerants. None of the scorable bands were observed in term of loss of original band of mother plant or gain of novel band...
Tipo: Journal article
Palavras-chave: AFLP; DNA methylation; In vitro propagation; MSAP; Potato; Somatic hybrids.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600005

Effect of nickel chloride on Arabidopsis genomic DNA and methylation of 18S rDNA
Provedor de dados: Electronic Journal of Biotechnology
Autores: Li,Zhongai; Chen,Xin; Li,Suoping; Wang,Zicheng.
Background In recent years, nickel (Ni) has been widely applied in industrial and agricultural production and has become a kind of environmental pollution. In this study, the effect of nickel chloride (NiCl2) with different concentrations on Arabidopsis genomic stability and DNA methylation has been demonstrated. The nucleolus variation and 18S rDNA methylation after NiCl2 treatment have been analyzed. Results The results are as follows: (1) The NiCl2 could result in heritable genomic methylation variations. The genomic DNA methylation variations have been detected by methylation-sensitive amplified polymorphism (MSAP) molecular markers, and the result showed that after NiCl2 treatment, there was methylation variation in T0 generation seedlings, and...
Tipo: Journal article
Palavras-chave: DNA methylation; Heavy metal; Methylation sensitive amplified polymorphism (MSAP); Nickel.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100010

The MTHFR C677T polymorphism and global DNA methylation in oral epithelial cells
Provedor de dados: Genetics and Molecular Biology
Autores: Arruda,Isabela Tatiana Sales de; Persuhn,Darlene Camati; Oliveira,Naila Francis Paulo de.
DNA methylation is mediated by DNA methyltransferases (DNMTs) that add a methyl group to the 5'-carbon of cytosine. The enzyme methylenetetrahydrofolate reductase (MTHFR) catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the rate-limiting step of the cycle involving the methyl donor S-adenosyl-L-methionine (SAM). The MTHFR C677T polymorphism results in a thermolabile enzyme with reduced activity that is predicted to influence the DNA methylation status. In this study, we investigated the impact of the MTHFR C677T polymorphism on the global DNA methylation of oral epithelial cells obtained from 54 healthy subjects. There were no significant differences in global DNA methylation among the MTHFR CC, CT and TT genotypes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation; Epigenetic; MTHFR C677T; Oral epithelial cells; Polymorphism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400004

Molecular characterization and transcription analysis of DNA methyltransferase genes in tomato (Solanum lycopersicum)
Provedor de dados: Genetics and Molecular Biology
Autores: Guo,Xuhu; Xie,Qian; Li,Baoyuan; Su,Huanzhen.
Abstract DNA methylation plays an important role in plant growth and development, gene expression regulation, and maintenance of genome stability. However, only little information regarding stress-related DNA methyltransferases (MTases) genes is available in tomato. Here, we report the analysis of nine tomato MTases, which were categorized into four known subfamilies. Structural analysis suggested their DNA methylase domains are highly conserved, whereas the N-terminals are divergent. Tissue-specific analysis of these MTase genes revealed that SlCMT2, SlCMT3, and SlDRM5 were expressed higher in young leaves, while SlMET1, SlCMT4, SlDRM7, and SlDRM8 were highly expressed in immature green fruit, and their expression declined continuously with further fruit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation; Tissue-specific expression; Abiotic stress; Fruit ripening; Tomato.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100307

Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1
Provedor de dados: Genetics and Molecular Biology
Autores: Du,Yue-feng; Liang,Liang; Shi,Ying; Long,Qing-zhi; Zeng,Jin; Wang,Xin-yang; He,Da-lin.
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; DNA methylation; DNMT3; RNA interference.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100024

DNA methylation modulates H19 and IGF2 expression in porcine female eye
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Dongxu; Wang,Guodong; Yang,Hao; Liu,Haibo; Li,Cuie; Li,Xiaolan; Lin,Chao; Song,Yuning; Li,Zhanjun; Liu,Dianfeng.
Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H19/IGF2; Gene expression; DNA methylation; Parthenogenetic; Pig.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153

DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Patrícia Santos Pereira; Molffeta,Greice Andreoti; Araujo,Amélia Góes de; Zago,Marco Antônio; Silva Jr.,Wilson Araújo da.
The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CpG island; DNA methylation; CDKN2B.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005

H19DMR methylation analysis in patients with Beckwith-Wiedemann syndrome and isolated hemihyperplasia
Provedor de dados: Genetics and Molecular Biology
Autores: Gomes,Marcus Vinícius de Matos; Santos,Sílvio Avelino dos; Ramos,Ester Silveira.
Beckwith-Wiedemann syndrome (BWS) is a congenital overgrowth disorder of complex and heterogeneous etiology involving alterations in genomic imprinting. The cause of isolated hemihyperplasia (IHH) is unknown but might be due to partial or incomplete expression of BWS because both these conditions share predisposition for the same types of neoplasias. We investigated the methylation pattern of the putative imprinting control region H19DMR using peripheral blood from 12 patients, six with clinical features of BWS and six with IHH. All the patients had normal karyotypes and paternal uniparental disomy (UPD) was excluded in 10 informative cases. The normal H19DMR methylation pattern was found in eight informative patients, indicating that H19DMR methylation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Beckwith-Wiedemann syndrome; Isolated hemihyperplasia; Genomic imprinting; DNA methylation; Uniparental disomy; H19DMR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200005

Epigenetic modifications and their relation to caste and sex determination and adult division of labor in the stingless bee Melipona scutellaris
Provedor de dados: Genetics and Molecular Biology
Autores: Cardoso-Júnior,Carlos A.M.; Fujimura,Patrícia Tieme; Santos-Júnior,Célio Dias; Borges,Naiara Araújo; Ueira-Vieira,Carlos; Hartfelder,Klaus; Goulart,Luiz Ricardo; Bonetti,Ana Maria.
Abstract Stingless bees of the genus Melipona, have long been considered an enigmatic case among social insects for their mode of caste determination, where in addition to larval food type and quantity, the genotype also has a saying, as proposed over 50 years ago by Warwick E. Kerr. Several attempts have since tried to test his Mendelian two-loci/two-alleles segregation hypothesis, but only recently a single gene crucial for sex determination in bees was evidenced to be sex-specifically spliced and also caste-specifically expressed in a Melipona species. Since alternative splicing is frequently associated with epigenetic marks, and the epigenetic status plays a major role in setting the caste phenotype in the honey bee, we investigated here epigenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation; Histone modification; Caste development; Social bees; Genetic caste determination.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100061

Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
Provedor de dados: Genetics and Molecular Biology
Autores: Wu,Wen-Chih; Hsu,Chih-Hsiung; Kuan,Jen-Chun; Hsieh,Jih-Fu; Sun,Chien-An; Yang,Tsan; Wu,Chang-Chieh; Chou,Yu-Ching.
A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal cancer; DNA methylation; Stool test for colorectal cancer.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004

Notos - a galaxy tool to analyze CpN observed expected ratios for inferring DNA methylation types
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bulla, Ingo; Aliaga, Benoit; Lacal, Virginia; Bulla, Jan; Grunau, Christoph; Chaparro, Cristian.
Background: DNA methylation patterns store epigenetic information in the vast majority of eukaryotic species. The relatively high costs and technical challenges associated with the detection of DNA methylation however have created a bias in the number of methylation studies towards model organisms. Consequently, it remains challenging to infer kingdom-wide general rules about the functions and evolutionary conservation of DNA methylation. Methylated cytosine is often found in specific CpN dinucleotides, and the frequency distributions of, for instance, CpG observed/expected (CpG o/e) ratios have been used to infer DNA methylation types based on higher mutability of methylated CpG. Results: Predominantly model-based approaches essentially founded on...
Tipo: Text
Palavras-chave: Epigenetics; DNA methylation; Kernel density estimation; CpG o/e ratio; CpN o/e ratio.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00437/54816/71790.pdf

Developmental Methylome of the Medicinal Plant Catharanthus roseus Unravels the Tissue-Specific Control of the Monoterpene Indole Alkaloid Pathway by DNA Methylation
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Dugé De Bernonville, Thomas; Maury, Stéphane; Delaunay, Alain; Daviaud, Christian; Chaparro, Cristian; Tost, Jörg; O’connor, Sarah Ellen; Courdavault, Vincent.
Catharanthus roseus produces a wide spectrum of monoterpene indole alkaloids (MIAs). MIA biosynthesis requires a tightly coordinated pathway involving more than 30 enzymatic steps that are spatio-temporally and environmentally regulated so that some MIAs specifically accumulate in restricted plant parts. The first regulatory layer involves a complex network of transcription factors from the basic Helix Loop Helix (bHLH) or AP2 families. In the present manuscript, we investigated whether an additional epigenetic layer could control the organ-, developmental- and environmental-specificity of MIA accumulation. We used Whole-Genome Bisulfite Sequencing (WGBS) together with RNA-seq to identify differentially methylated and expressed genes among nine samples...
Tipo: Text
Palavras-chave: Alkaloids; DNA methylation; Epigenetics; Folivory; Organs; Plant specialized metabolism.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00652/76453/77543.pdf

Cytogenetic evidences of genome rearrangement and differential epigenetic chromatin modification in the sea lamprey (Petromyzon marinus)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Covelo-soto, Lara; Moran, Paloma; Pasantes, Juan J.; Perez Garcia, Maria Concepcion.
This work explores both the chromatin loss and the differential genome methylation in the sea lamprey (Petromyzon marinus) from a molecular cytogenetic point of view. Fluorescent in situ hybridization experiments on meiotic bivalents and mitotic chromosomes corroborate the chromatin loss previously observed during the development of the sea lamprey and demonstrate that the elimination affects not only to Germ1 sequences but also to the rpt200 satellite DNA and most part of the major ribosomal DNA present on the germinal line. 5-Methylcytosine immunolocation revealed that the GC-rich heterochromatin is highly methylated in the germ line but significantly less in somatic chromosomes. These findings not only support previous observations about genome...
Tipo: Text
Palavras-chave: Sea lamprey; Chromatin loss; Genome rearrangements; DNA methylation.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00247/35811/34327.pdf

Environmentally Driven Color Variation in the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus, 1758) Is Associated With Differential Methylation of CpGs in Pigment- and Biomineralization-Related Genes
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Stenger, Pierre-louis; Ky, Chin-long; Reisser, Celine; Cosseau, Céline; Grunau, Christoph; Mege, Mickael; Planes, Serge; Vidal-dupiol, Jeremie.
Today, it is common knowledge that environmental factors can change the color of many animals. Studies have shown that the molecular mechanisms underlying such modifications could involve epigenetic factors. Since 2013, the pearl oyster Pinctada margaritifera var. cumingii has become a biological model for questions on color expression and variation in Mollusca. A previous study reported color plasticity in response to water depth variation, specifically a general darkening of the nacre color at greater depth. However, the molecular mechanisms behind this plasticity are still unknown. In this paper, we investigate the possible implication of epigenetic factors controlling shell color variation through a depth variation experiment associated with a DNA...
Tipo: Text
Palavras-chave: Pearl oyster; Environmental pressure; Depth; Color change; Pigmentation; DNA methylation; Methylome characterization.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00685/79667/82436.pdf

The insecticide permethrin induces transgenerational behavioral changes linked to transcriptomic and epigenetic alterations in zebrafish (Danio rerio)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Blanc, Mélanie; Antczak, Philipp; Cousin, Xavier; Grunau, Christoph; Scherbak, Nikolai; Rüegg, Joëlle; Keiter, Steffen H.
The pyrethroid insecticide permethrin is widely used for agricultural and domestic purposes. Previous data indicated that it acts as a developmental neurotoxicant and can induce transgenerational effects in non-target organisms. However, associated underlying mechanisms remain unclear. The aim of this study was to investigate permethrin-related transgenerational effects in the zebrafish model, and to identify possible molecular mechanisms underlying inheritance. Zebrafish (F0) were exposed to permethrin during early-life (2 h post-fertilization up to 28 days). The F1 and F2 offspring generations were obtained by pairing exposed F0 males and females, and were bred unexposed. Locomotor and anxiety behavior were investigated, together with transcriptomic and...
Tipo: Text
Palavras-chave: Transgenerational epigenetic inheritance; Developmental neurotoxicity; Glutamatergic signaling; DNA methylation; Pesticide.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00685/79661/82425.pdf

Dynamic epimarks in sex-related genes predict gonad phenotype in the European sea bass, a fish with mixed genetic and environmental sex determination
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Anastasiadi, Dafni; Vandeputte, Marc; Sanchez-baizan, Nuria; Allal, Francois; Piferrer, Francesc.
The integration of genomic and environmental influences into methylation patterns to bring about a phenotype is of central interest in developmental epigenetics, but many details are still unclear. The sex ratios of the species used here, the European sea bass, are determined by genetic and temperature influences. We created four families from parents known to produce offspring with different sex ratios, exposed larvae to masculinizing temperatures and examined, in juvenile gonads, the DNA methylation of seven genes related to sexual development by a targeted sequencing approach. The genes most affected by both genetics and environment were cyp19a1a and dmrt1, with contrasting sex-specific methylation and temperature responses. The relationship between...
Tipo: Text
Palavras-chave: Early development; Environmental temperature; DNA methylation; Sex determination; Aromatase; Cyp19a1a; Dmrt1; Epigenetic marks; DNA methylation threshold; Epigenetic inheritance.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00470/58123/61657.pdf

Effects of a parental exposure to diuron on Pacific oyster spat methylome
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rondon Sallan, Rodolfo; Grunau, Christoph; Fallet, Manon; Charlemagne, Nicolas; Sussarellu, Rossana; Chaparro, Cristian; Montagnani, Caroline; Mitta, Guillaume; Bachere, Evelyne; Akcha, Farida; Cosseau, Celine.
Environmental epigenetic is an emerging field that studies the cause-effect relationship between environmental factors and heritable trait via an alteration in epigenetic marks. This field has received much attentions since the impact of environmental factors on different epigenetic marks have been shown to be associated with a broad range of phenotypic disorders in natural ecosystems. Chemical pollutants have been shown to affect immediate epigenetic information carriers of several aquatic species but the heritability of the chromatin marks and the consequences for long term adaptation remain open questions. In this work, we investigated the impact of the diuron herbicide on the DNA methylation pattern of spat from exposed Crassotrea gigas genitors. This...
Tipo: Text
Palavras-chave: Crassostrea gigas; Mollusk; DNA methylation; Herbicide; Epigenetic.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00602/71413/69895.pdf

Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Sussarellu, Rossana; Lebreton, Morgane; Rouxel, Julien; Akcha, Farida; Riviere, Guillaume.
Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development. For that purpose, oyster embryos were exposed to 4 nominal copper concentrations (0.1, 1, 10 and 20 μg L−1 Cu2+) during early development assays. Embryotoxicity was monitored through the oyster embryo-larval...
Tipo: Text
Palavras-chave: Oyster; Embryotoxicity; Copper; Gene expression; DNA methylation.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00417/52856/53756.pdf

The Seagrass Methylome Is Associated With Variation in Photosynthetic Performance Among Clonal Shoots
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Jueterbock, Alexander; Boström, Christoffer; Coyer, James A.; Olsen, Jeanine L.; Kopp, Martina; Dhanasiri, Anusha K. S.; Smolina, Irina; Arnaud-haond, Sophie; Van De Peer, Yves; Hoarau, Galice.
Evolutionary theory predicts that clonal organisms are more susceptible to extinction than sexually reproducing organisms, due to low genetic variation and slow rates of evolution. In agreement, conservation management considers genetic variation as the ultimate measure of a population’s ability to survive over time. However, clonal plants are among the oldest living organisms on our planet. Here, we test the hypothesis that clonal seagrass meadows display epigenetic variation that complements genetic variation as a source of phenotypic variation. In a clonal meadow of the seagrass Zostera marina, we characterized DNA methylation among 42 shoots. We also sequenced the whole genome of 10 shoots to correlate methylation patterns with photosynthetic...
Tipo: Text
Palavras-chave: DNA methylation; Ecological epigenetics; Clonality; Heat stress; Seagrass; Zostera marina(eelgrass).
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00652/76375/77363.pdf

Position-dependent correlation between TBX22 exon 5 methylation and palatal shelf fusion in the development of cleft palate
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: LI,KE; SHU,XUAN; GONG,HUI; CHENG,LIUHANGHANG; DONG,ZEJUN; SHU,SHENYOU.
Abstract: DNA methylation is essential for spatiotemporally-regulated gene expression in embryonic development. TBX22 (Chr X: 107667964-107688978) functioning as a transcriptional repressor affects DNA binding, sumoylation, and transcriptional repression associated with X-linked cleft palate. This study aimed to explore the relationship and potential mechanism between TBX22 exon 5 methylation and palatal shelf fusion ﻿induced by all-trans retinoic acid (ATRA). ﻿We performed DNA methylation profiling, using MethylRAD-seq, after high throughput sequencing of mouse embryos from control (n=9) and ATRA-treated (to induce cleft palate, n=9) C57BL/6J mice at embryonic gestation days(E) 13.5, 14.5 and 16.5. ﻿TBX22 exon 5 was hyper-methylated﻿ at the CpG site at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation; Cleft palate; TBX22; CpG site.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300711

Schizophrenia and reelin: a model based on prenatal stress to study epigenetics, brain development and behavior
Provedor de dados: Biological Research
Autores: Negrón-Oyarzo,Ignacio; Lara-Vásquez,Ariel; Palacios-García,Ismael; Fuentealba,Pablo; Aboitiz,Francisco.
Schizophrenia is a severe psychiatric disorder that results in a significant disability for the patient. The disorder is characterized by impairment of the adaptive orchestration of actions, a cognitive function that is mainly dependent on the prefrontal cortex. This behavioral deficit, together with cellular and neurophysiological alterations in the prefrontal cortex, as well as reduced density of GABAergic cells and aberrant oscillatory activity, all indicate structural and functional deficits of the prefrontal cortex in schizophrenia. Among the several risk factors for the development of schizophrenia, stress during the prenatal period has been identified as crucial. Thus, it is proposed that prenatal stress induces neurodevelopmental alterations in the...
Tipo: Journal article
Palavras-chave: Schizophrenia; Prefrontal cortex; Prenatal stress; Functional connectivity; DNA methylation; Reelin.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100016

Correlations of recognition memory performance with expression and methylation of brain-derived neurotrophic factor in rats
Provedor de dados: Biological Research
Autores: Muñoz,Pablo C; Aspé,Mauricio A; Contreras,Luis S; Palacios,Adrián G.
Object recognition memory allows discrimination between novel and familiar objects. This kind of memory consists of two components: recollection, which depends on the hippocampus, and familiarity, which depends on the perirhinal cortex (Pcx). The importance of brain-derived neurotrophic factor (BDNF) for recognition memory has already been recognized. Recent evidence suggests that DNA methylation regulates the expression of BDNF and memory. Behavioral and molecular approaches were used to understand the potential contribution of DNA methylation to recognition memory. To that end, rats were tested for their ability to distinguish novel from familiar objects by using a spontaneous object recognition task. Furthermore, the level of DNA methylation was...
Tipo: Journal article
Palavras-chave: DNA methylation; Recognition memory; Synaptic plasticity; Hippocampal; Perirhinal cortex; BDNF.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200013

Biomarkers towards Ovarian Cancer Diagnostics: Present and Future Prospects
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Rastogi,Meenal; Gupta,Sameer; Sachan,Manisha.
ABSTRACT Ovarian carcinoma accounts for highest mortality of all gynecologic malignancies as the disease is asymptomatic until late stages. Biomarkers such as CA-125 and HE4 are being currently used for diagnosis of ovarian cancer, but they show contradicting diagnostic accuracy. Therefore other biomarkers have been investigated for early detection of this disease, but no success has been obtained and no test has yet been recommended for screening a general population. In this instance, aptamers can be effectively used to identify tumor-specific antigens for early diagnosis and targeted therapy of ovarian cancer. This article provides an overview of the biomarkers/panels being explored as well as the potential of aptamers to improve current long-term...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovarian cancer; Aptamers; DNA methylation; CA-125; HE4.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100346

Age-related Changes in DNA Methylation Status of hTERT Gene Promoter of Oral Epithelial Cells
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Bezerra,Stephane Flaviane de Oliveira; Costa,Ludimila de Araújo; Freitas,Priscylla Alves Nascimento de; Oliveira,Naila Francis Paulo de.
The purpose of this study was to investigate the effect of aging on the DNA methylation status of two genes involved in tumorigenesis (telomerase gene hTERT and DNA repair gene- MLH1) and one in metabolism (methylenetetrahydrofolate reductase gene- MTHFR) in oral epithelial cells. DNA methylation analysis was performed by Methylation Sensitive Restriction Enzymes (MSRE) of healthy oral epithelial cells of child (6-10 years, n=21), young (20-25 years, n=19) and elderly (over 60 years, n=25). The results for the hTERT gene showed significant variation in the methylation frequency at CpG dinucleotides among the groups (p=0.0001), with the methylated condition more frequently in children and young people. In relation to MLH1 and MTHFR, no differences were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigenetic; DNA methylation; Aging; DNA repair; Telomerase; MTHFR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100082

Cryptic mosaicism involving a second chromosome X in patients with Turner syndrome
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Araújo,A.; Ramos,E.S.
The high abortion rate of 45,X embryos indicates that patients with Turner syndrome and 45,X karyotype could be mosaics, in at least one phase of embryo development or cellular lineage, due to the need for the other sex chromosome presence for conceptus to be compatible with life. In cases of structural chromosomal aberrations or hidden mosaicism, conventional cytogenetic techniques can be ineffective and molecular investigation is indicated. Two hundred and fifty patients with Turner syndrome stigmata were studied and 36 who had female genitalia and had been cytogenetically diagnosed as having "pure" 45,X karyotype were selected after 100 metaphases were analyzed in order to exclude mosaicism and the presence of genomic Y-specific sequences (SRY, TSPY,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Turner syndrome; X-inactivation; HUMARA assay; DNA methylation; Criptic mosaicism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500004

Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lima,E.M.; Leal,M.F.; Burbano,R.R.; Khayat,A.S.; Assumpção,P.P.; Bello,M.J.; Rey,J.A.; Smith,M.A.C.; Casartelli,C.
Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation; Gastric cancer; ANAPC1; CDKN2A; TP53.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600017

Stability of XIST repression in relation to genomic imprinting following global genome demethylation in a human cell line
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: de Araújo,E.S.S.; Vasques,L.R.; Stabellini,R.; Krepischi,A.C.V.; Pereira,L.V.
DNA methylation is essential in X chromosome inactivation and genomic imprinting, maintaining repression of XIST in the active X chromosome and monoallelic repression of imprinted genes. Disruption of the DNA methyltransferase genes DNMT1 and DNMT3B in the HCT116 cell line (DKO cells) leads to global DNA hypomethylation and biallelic expression of the imprinted gene IGF2 but does not lead to reactivation of XIST expression, suggesting thatXIST repression is due to a more stable epigenetic mark than imprinting. To test this hypothesis, we induced acute hypomethylation in HCT116 cells by 5-aza-2′-deoxycytidine (5-aza-CdR) treatment (HCT116-5-aza-CdR) and compared that to DKO cells, evaluating DNA methylation by microarray and monitoring the expression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: XIST; Imprinted genes; DNA methylation; 5-aza-2′-deoxycytidine; Human cell line.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201029

The prima donna of epigenetics: the regulation of gene expression by DNA methylation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Santos,K.F.; Mazzola,T.N.; Carvalho,H.F.
This review focuses on the mechanisms of DNA methylation, DNA methylation pattern formation and their involvement in gene regulation. Association of DNA methylation with imprinting, embryonic development and human diseases is discussed. Furthermore, besides considering changes in DNA methylation as mechanisms of disease, the role of epigenetics in general and DNA methylation in particular in transgenerational carcinogenesis, in memory formation and behavior establishment are brought about as mechanisms based on the cellular memory of gene expression patterns.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigenetics; DNA methylation; Inheritable changes in gene expression; Transgenerational carcinogenesis; Environmental influence on memory formation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000010