Consulta: subjectFacets:"Danio rerio"
Registros recuperados: 9
Data/hora: 08/06/2024 11:29:58

Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish—part II: behavior
Provedor de dados: 5
Autores: Vignet, Caroline; Le Menach, Karyn; Lyphout, Laura; Guionnet, Tiphaine; Frere, Laura; Leguay, Didier; Budzinski, Helene; Cousin, Xavier; Begout, Marie-laure.
In the last 10 years, behavior assessment has been developed as an indicator of neurotoxicity and an integrated indicator of physiological disruption. Polycyclic aromatic hydrocarbon (PAH) release into the environment has increased in recent decades resulting in high concentrations of these compounds in the sediment of contaminated areas. We evaluated the behavioral consequences of long-term chronic exposure to PAHs, by exposing zebrafish to diets spiked with three PAH fractions at environmentally relevant concentrations. Fish were exposed to these chemicals from their first meal (5 days postfertilization) until they became reproducing adults (at 6 months old). The fractions used were representative of PAHs of pyrolytic (PY) origin and of two oils...
Tipo: Text
Palavras-chave: Danio rerio; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Anxiety-like behavior; Photomotor responses; Maze; Novel tank.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00188/29910/28380.pdf

Long-term disruption of growth, reproduction, and behavior after embryonic exposure of zebrafish to PAH-spiked sediment
Provedor de dados: 5
Autores: Vignet, Caroline; Devier, Marie-hélène; Le Menach, Karyn; Lyphout, Laura; Potier, Jeremy; Cachot, Jerome; Budzinski, Helene; Begout, Marie-laure; Cousin, Xavier.
A natural sediment spiked with three individual polycyclic aromatic hydrocarbons (PAHs; pyrene, phenanthrene and benzo[a]pyrene) was used to expose zebrafish embryos and larvae during 4 days. The total PAH concentration was 4.4 μg g−1 which is in the range found in sediment from contaminated areas. Quantification of metabolites in the larvae after exposure confirmed the actual contamination of the larvae and indicated an active metabolism especially for pyrene and benzo[a]pyrene. After a transfer in a clean medium, the larvae were reared to adulthood and evaluated for survival, growth, reproduction, and behavior. Measured endpoints revealed a late disruption of growth (appearing at 5 months) and a trend toward a lower reproductive ability. Adults of...
Tipo: Text
Palavras-chave: Danio rerio; Polycyclic aromatic hydrocarbon; Lifecycle; Anxiety-like behavior; Offspring.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00188/29909/28497.pdf

Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish - part I: Survival and growth
Provedor de dados: 5
Autores: Vignet, Caroline; Le Menach, Karyn; Mazurais, David; Lucas, Julie; Perrichon, Prescilla; Le Bihanic, Florane; Devier, Marie-hélène; Lyphout, Laura; Frere, Laura; Begout, Marie-laure; Zambonino-infante, Jose Luis; Budzinski, Helene; Cousin, Xavier.
The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6–6.7 μg g−1 for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel...
Tipo: Text
Palavras-chave: Danio rerio; Life cycle; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Alkylated PAH; Jaw; Digestion.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00188/29907/28490.pdf

Exposures of zebrafish through diet to three environmentally relevant mixtures of PAHs produce behavioral disruptions in unexposed F1 and F2 descendant
Provedor de dados: 5
Autores: Vignet, Caroline; Joassard, Lucette; Lyphout, Laura; Guionnet, Tiphaine; Goubeau, Manon; Le Menach, Karyn; Brion, Francois; Kah, Olivier; Chung, Bon-chu; Budzinski, Helene; Begout, Marie-laure; Cousin, Xavier.
The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades. PAHs are hydrophobic molecules which can accumulate in high concentrations in sediments acting then as major secondary sources. Fish contamination can occur through contact or residence nearby sediments or though dietary exposure. In this study, we analyzed certain physiological traits in unexposed fish (F1) issued from parents (F0) exposed through diet to three PAH mixtures at similar and environmentally relevant concentrations but differing in their compositions. For each mixture, no morphological differences were observed between concentrations. An increase in locomotor activity was observed in larvae issued from fish...
Tipo: Text
Palavras-chave: Danio rerio; Polycyclic aromatic hydrocarbon; Offspring; Transgenerational effect; Heavy oil; Light crude oil; Locomotor activity; Photomotor response; Anxiety-like behavior.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00259/37030/35688.pdf

An environmentally relevant mixture of polychlorinated biphenyls (PCBs) and polybrominated diphenylethers (PBDEs) disrupts mitochondrial function, lipid metabolism and neurotransmission in the brain of exposed zebrafish and their unexposed F2 offspring
Provedor de dados: 5
Autores: Blanc, Mélanie; Alfonso, Sebastien; Bégout, Marie-laure; Barrachina, Célia; Hyötyläinen, Tuulia; Keiter, Steffen H.; Cousin, Xavier.
Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants still present in aquatic environments despite their total or partial ban. Previously, we observed that an environmentally realistic mixture of these compounds affects energy balance, growth, and reproduction in exposed zebrafish (F0), and behavior in their unexposed offspring (F1-F4). In the present work, we performed lipidomic and transcriptomic analyses on brains of zebrafish (F0-F2) from exposed and control lineages to identify molecular changes that could explain the observed phenotypes. The use of both technologies highlighted that F0 zebrafish displayed impaired mitochondrial function and lipid metabolism regulation (depletion in...
Tipo: Text
Palavras-chave: Danio rerio; Persistent-organic pollutant; Neurotoxicity; Brain metabolism; RNA-Seq.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00646/75797/76720.pdf

Altération de la physiologie des poissons exposés à des hydrocarbures aromatiques polycycliques (HAP). Comportement et reproduction
Provedor de dados: 5
Autores: Vignet, Caroline.
Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants (POPs) which are found in complex mixtures in the environment including aquatic ecosystems. They adsorb on particles, accumulate in sediment and in the first link of the food web. The aim of this study was to measure long term effects of PAH scontamination on a vertebrate model: the zebrafish. In a first study, embryos were exposed on natural sediment spiked with 3 individual PAH (phenanthrene, pyrene and benzo[a]pyrene) during the first four days of their development. In a second study, to evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal (5 days post fertilisation) until they became reproducing adults, to diets spiked...
Tipo: Text
Palavras-chave: Danio rerio; Lifecycle; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Alkylated PAH; Lifecycle; Delayed effect; Anxiety-like behavior; Offspring; Photomotor responses; Maze; Novel tank.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00195/30598/29052.pdf

Alterations on growth and gill morphology of Danio rerio (Pisces, Ciprinidae) exposed to the toxic sediments
Provedor de dados: 52
Autores: Fracácio,Renata; Verani,Nelsy Fenerich; Espíndola,Evaldo Luiz Gaeta; Rocha,Odete; Rigolin-Sá,Odila; Andrade,Cássio Arilson.
The objective of this work was to assess the toxicity of sediment samples from six cascade reservoirs in Tietê river system, in São Paulo State, Brazil, through chronic-partial toxicity bioassays with Danio rerio larvae as test-organisms. Histology of gills and biometric measurements were used to determine the existence of toxicity problems. The alterations on gill morphology here detected were considered of first stage (hyperplasia, lamellar junction and excess of mucous cells) in the first reservoirs and very slight in the last ones. The biometric analysis pointed to inadequate conditions for the growth of the test-organisms when exposed to the sediment of the rivers and upstream reservoirs and also indicated an improvement of environmental conditions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gill morphology; Sediment toxicity; Danio rerio; Tietê reservoirs; Pollution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400023

Analyses of the sediment toxicity of Monjolinho River, São Carlos, São Paulo State, Brazil, using survey, growth and gill morphology of two fish species (Danio rerio and Poecilia reticulata)
Provedor de dados: 52
Autores: Campagna,Aline Fernanda; Fracácio,Renata; Rodrigues,Beatriz Kawamura; Eler,Márcia Noélia; Verani,Nelsy Fenerich; Espíndola,Evaldo Luiz Gaeta.
The objective of this work was to evaluate the toxicity of the sediments of the Monjolinho River (São Carlos - São Paulo/Brazil), through partial chronic toxicity bioassays with juveniles of D. rerio and P. reticulata. Histological analyses of the gills and biometric measurements were conducted to detect the possible deleterious effects that caused the death. In all points the sampled the alterations were found in the gills (hyperplasia, fusion of secondary lamellas and dilation of blood vessels), with the most intense lesions (second stage) occurring at the point two. The biometric analysis pointed to inadequate conditions for the growth of the test-organisms when exposed to the sediment samples. These results showed that the conditions of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sediment Toxicity; Danio rerio; Monjolinho River; Poecilia reticulata.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100023

L-histidine enhances learning in stressed zebrafish
Provedor de dados: 56
Autores: Cofiel,L.P.V.; Mattioli,R.
The aim of the present study was to determine the effect of the histaminergic precursor L-histidine and the H3 receptor antagonist thioperamide on the learning process of zebrafish submitted or not to confinement stress. On each of the 5 consecutive days of experiment (D1, D2, D3, D4, D5), animals had to associate an interruption of the aquarium air supply with food offering. Non-stressed zebrafish received an intraperitoneal injection of 100 mg/kg L-histidine, 10 mg/kg thioperamide or saline after training. Stressed animals received drug treatment and then were submitted to confinement stress for 1 h before the learning procedure. Time to approach the feeder was measured (in seconds) and was considered to be indicative of learning. A decrease in time to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Danio rerio; Conditioning; Feed trial; Thioperamide; H3 receptor; Blood glucose.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100018