Consulta: subjectFacets:"Diagnosis"
Registros recuperados: 230
Data/hora: 28/05/2024 15:24:39

Diagnostico geomorfológico en cuencas hidrográficas precordilleranas: Río de la Ciénaga. Departamento Zonda, Provincia de San Juan, República Argentina
Provedor de dados: 25
Autores: Montenegro, Elvira Aideé Suarez.
The results exposed in the present work are framed in the activities of Internal Scholarship of CONICET that is developed in the Institute of Geography Applied in National San Juan University. The General Objective of the investigation work was based on application of the systemic approach, for the analysis of natural systems of “precordilleranas” hydrographic river basins. In this case it was chosen like object focused to the geomorphologic object. Satellite Techniques, Manual and Digital Mapping with ITC Symbol, Direct Wide Observations and SIG with Software Ilwis 3.3 Were used . It was selected as study unit the area drained by the river, that is located in Central Precordillera of San Juan Province, in virtue to fort influences new tectonics,...
Tipo: Article
Palavras-chave: Natural System; Geomorphology; Diagnosis.
Ano: 2007
URL: http://hdl.handle.net/2315/114

Diagnóstico del manejo postcosecha, comercialización e industrialización de piña (Ananas comosus L. Merill) en Huimanguillo Tabasco, México
Provedor de dados: 32
Autores: Cano Ascencio, Héctor.
El municipio de Huimanguillo, Tabasco es la tercera región productora de piña en México y no esta exenta de la problemática generalizada en dicho sector (socioeconómicos, tecnológicos y ambientales); por lo que con el presente trabajo se planteó conocer a detalle mediante un diagnóstico la situación sobre el manejo pre y post cosecha, comercialización e industrialización de piña. Los métodos de investigación utilizados fueron cuantitativos y cualitativos; para lo cual mediante un muestreo simple aleatorio (MSA) se seleccionaron a 72 productores y se aplicó una encuesta, donde el instrumento para registrar la información de las variables de estudio fue el cuestionario; de igual forma se aplicaron encuestas a comercializadores e industriales de la zona. El...
Tipo: Tesis
Palavras-chave: Diagnóstico; Inocuidad; Manejo; Piña; Diagnosis; Food safety; Handling; Pineapple.
Ano: 2008
URL: http://hdl.handle.net/10521/1579

Diagnóstico del manejo postcosecha, comercialización e industrialización de piña (Ananas comosus L. Merill) en Huimanguillo, Tabasco, México
Provedor de dados: 32
Autores: Cano Ascencio, Héctor.
El municipio de Huimanguillo, Tabasco es la tercera región productora de piña en  México y no esta exenta de la problemática generalizada en dicho sector  (socioeconómicos, tecnológicos y ambientales); por lo que con el presente trabajo se  planteó conocer a detalle mediante un diagnóstico la situación sobre el manejo pre y  post cosecha, comercialización e industrialización de piña. Los métodos de  investigación utilizados fueron cuantitativos y cualitativos; para lo cual mediante un  muestreo simple aleatorio (MSA) se seleccionaron a 72 productores y se aplicó una  encuesta, donde el instrumento para registrar la información de las variables de estudio  fue el cuestionario; de igual forma se aplicaron encuestas a comercializadores e  industriales de la...
Tipo: Tesis
Palavras-chave: Diagnóstico; Inocuidad; Manejo; Piña; Maestría; Producción Agroalimentaria en el Trópico; Diagnosis; Food safety; Handling; Pineapple.
Ano: 2008
URL: http://hdl.handle.net/10521/1419

Diagnosis of patients with Prader-Willi and Angelman Syndromes: the importance of an overall investigation
Provedor de dados: 74
Autores: Varela,Monica Castro; Fridman,Cintia; Koiffmann,Célia Priszkulnik.
Seventy-two patients with clinical diagnoses of Prader-Willi (PWS; n = 28 patients) or Angelman syndromes (AS; n = 44 patients) were submitted to chromosome analysis, SNRPN-SNURF exon 1 methylation assay, and microsatellite genotyping. Analysis of the methylation pattern confirmed the PWS diagnosis in 18 out of 28 patients and the AS diagnosis in 20 out of 44 patients. FISH and microsatellite analysis detected a deletion in 30 patients (14 PWS and 16 AS). Eight patients had normal FISH results (4 PWS and 4 AS); microsatellite markers showed that these patients had a uniparental disomy (UPD). Based on this study, we propose a strategy for the routine diagnosis of these syndromes that consists of the following steps: 1) methylation analysis, which does not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angelman syndrome; Prader-Willi syndrome; Diagnosis; 15q deletion; Uniparental disomy; Genomic imprinting.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100003

[RETRACTED ARTICLE]: Expression of serum microRNA-378 and its clinical significance in renal cell carcinoma
Provedor de dados: 74
Autores: Shi,Lixin; Zhang,Lei; Wang,Chunyang; Sun,Shengkun; Cao,Xiyuan; Zhang,Xu.
Abstract Studies have demonstrated that miRNA-378 is expressed in various malignant tumors. In the present study, we aimed to explore the expression of serum miRNA-378 and its clinical significance in renal cell carcinoma (RCC) patients. A total of 75 RCC patients, 63 renal cysts (RC) patients and 75 healthy controls were selected. The miRNA-378 level in RCC and RC groups was significantly higher than in healthy control group, with RCC group having the highest level. The miRNA-378 levels were significantly decreased within the same group after surgery. When compared with healthy controls, RC group had higher levels but not significantly (p &gt; 0.05) while levels in RCC group were significantly higher (p &lt; 0.05). miRNA-378 expression was correlated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal cell carcinoma; MicroRNA 378; Diagnosis; Patients; AUC.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300525

Characterization of the Brazilian vegetable brassicas production chain
Provedor de dados: 75
Autores: Melo,Raphael AC; Vendrame,Larissa PC; Madeira,Nuno R; Blind,Ariel D; Vilela,Nirlene J.
ABSTRACT Brassica crops worldwide provide the greatest diversity of products used by man derived from a single genus. Collectively, leafy, flower and root vegetables can be eaten fresh, cooked and processed. The production of these vegetables in Brazil has been growing exponentially in the last decades, due to its economic importance, with major production volumes in several states and a quick monetary return for growers in areas considered of small acreage. With expansion, a number of aspects, including demands for quality and safety of these foods introduce themselves, strictly attached to the forms of production, logistics and their appearance. To meet the challenges and list the trends within the activities of different agents, a multiple case study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassica oleracea; Diagnosis; Scenario; Regions.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000400366

The multifunctionality of urban horticulture and its integration with the city ecosystem: a brief review of concepts and the case of São Luís
Provedor de dados: 75
Autores: Gomes,João Flávio B; Gomes,Renata SB; Souza,Alex O.
ABSTRACT Urban agriculture is currently carried out all over the world. The activity is characterized by its multifunctionality, contributing to food security, preservation of biodiversity, better use of urban spaces, and proper management of soil and water, in addition to contributing to increasing income and improving the quality of life of farmers who live in the cities. In the 1980s and 1990s, urban agriculture gained momentum on the international scene and, from 2005, in Brazil. Some successful experiences in the world (Detroit, Havana, and St. Petersburg) and in Brazil (Teresina, Sao Paulo, and Belo Horizonte) are briefly reported here. Then, we describe in more detail the case of the city of São Luís. The municipality is situated on an island and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urban- and peri-urban agriculture; Public policies; Production chains; Socioeconomic analysis; Diagnosis; Scenarios.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000300252

Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
Provedor de dados: 75
Autores: Barros,Aline F; Campos,Vicente P; Souza,Larissa N; Costa,Sarah S; Terra,Willian C; Lessa,Josimar HL.
ABSTRACT Species of the genus Meloidogyne are limiting factors in vegetable crop production. Studies in Brazil about the occurrence of root-knot nematodes in areas of vegetable crop growth have been conducted without using advanced techniques. Using modern techniques, such as biochemical and molecular methods, improves the accuracy of Meloidogyne species identification. The present study characterized species of Meloidogyne in 36 samples associated with vegetable crops using isoenzyme electrophoresis, SCAR markers, and morphological markers, in addition to validating SCAR markers for accurate species identification. The species M. incognita, M. javanica, M. hapla, M. morocciensis, and M. arenaria were identified, with the first two being the most frequent....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meloidogyne; Diagnosis; Molecular biology; Vegetable crops.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400473

Harvest-Expedition on Protected Cultivation: characterization and prospective study of the challenges and solutions associated with the protected cultivation of vegetable crops
Provedor de dados: 75
Autores: Vieira,Débora de FA; Clemente,Flávia Maria VT.
ABSTRACT Farmers and the rural extension service in Planaltina, in the Federal District, an important pole of tomato and sweet pepper production in the Brazilian Midwest region, have been observing decreases in yield in protected cultivation lately. Yields are getting back to those registered in initial years of the system’s implementation in the region. Besides, prices paid to farmers dropped due to the retreat in fruit and vegetable consumption by Brazilians registered since 2015, triggering a crisis in the region. The so-called “Harvest-Expedition on Protected Cultivation” brought together Embrapa (Brazilian Agricultural Research Corporation), Emater-DF (Federal District Agency for Technical Assistance and Rural Extension) and SEAGRI-DF (State...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Tomato; Sweet pepper; Management; Scenarios.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400431

Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
Provedor de dados: 75
Autores: Pontes,Nadson C; Fujinawa,Miriam F; Oliveira,José R.
ABSTRACT The efficacy of five selective media described in the literature for the detection of Ralstonia solanacearum in soil was evaluated using isolates from different regions of Brazil. The selective media were evaluated regarding their sensitivity, target-pathogen repression index, capacity for suppressing contaminating microorganisms and recovery rate of the pathogen in soil. No bacterial growth was observed at the concentrations tested for FSM and SM-1 media. Some isolates grew on the PCCG medium with the addition of antimicrobial agents, but only at concentrations of 106 cfu/mL. For the SM and SMSA-E media, adequate sensitivity for the detection of R. solanacearum in soil was observed, enabling detection at a concentration of approximately 103...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial wilt; Culture medium; Diagnosis; SMSA-E.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000100041

Detection of Cucumber mosaic virus in commercial anthurium crops and genotypes evaluation
Provedor de dados: 75
Autores: Miura,Nancy de S; Beriam,Luís OS; Rivas,Eliana B.
In Brazil, Anthurium andraeanum (flamingo flower) stands out as an important species for cut leaves and flowers productions, as well as potted cultivation. Due to the fact that ornamental plants play an important part in the tropical agriculture diversification, there is great interest to know the problems that affect anthurium cultivation in order to improve its productive potential. The aims of this study were identify the causal agent of intense mosaic and leaf deformation observed in anthurium commercial crops from four cities of the São Paulo state, Brazil; to assess the extent of the virus occurrence in these cities and the methods for its detection, as well as to analyse the anthurium genotypes reactions in relation to the virus. Anthuriums were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthurium andraeanum; Araceae; Cucumovirus; Diagnosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200024

Genetic resources of vegetable crops: a survey in the Brazilian germplasm collections pictured through papers published in the journals of the Brazilian Society for Horticultural Science
Provedor de dados: 75
Autores: Sudré,Cláudia P; Leonardecz,Eduardo; Rodrigues,Rosana; Amaral Júnior,Antônio T do; Moura,Maria da CL; Gonçalves,Leandro SA.
The research on plant genetic resources is essential for the conservation of genetic diversity and accessions' divergence studies, as a basis for plant breeding. Aiming to know the state of art in this subject, a historical survey was carried out in Revista de Olericultura and in Horticultura Brasileira, from 1961 to 2006, searching for papers dealing with vegetable crops genetic resources. In each of the papers, the species studied, first author institution, publication year, applied software, number of accessions and descriptors, and the multivariate techniques used were registered. Based on these characteristics, papers were grouped using multivariate analysis. Sixty-one papers dealt somehow with genetic resources in the time covered by the survey, from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Review; Multivariate analysis; Diagnosis; Genetic diversity; Vegetables.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362007000400002

Redescripción de Drosophila ogradi y descripción de una especie nueva del grupo Drosophila morelia (Diptera, Drosophilidae)
Provedor de dados: 76
Autores: Cabezas,María Beatriz; Rafael,Violeta.
RESUMEN En el presente estudio se analizaron y compararon los caracteres morfológicos de una especie de Drosophila, capturada en el bosque nublado de Intillacta, Pichincha, Ecuador. Los análisis de los rasgos morfológicos como manchas de las alas y genitalia sugieren que se trata de una especie nueva perteneciente al grupo Drosophila morelia. Por lo tanto, Drosophila nina sp. nov., descrita en el presente estudio, es la quinta especie de este grupo. Así mismo se redescribe a Drosophila ogradi Vela &amp; Rafael, 2004 para completar la información faltante en la descripción original.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecuador; Bosque nublado; Intillacta; Diagnosis; Genitalia.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212015000200157

Diagnostic et zanage agricole municipal: le cas de Juazeiro.
Provedor de dados: 14
Autores: SABOURIN, E.; CARON, P.; OLIVEIRA, J. S.; ALVES, R. S.
bitstream/item/196699/1/Diagnostic-et-zonage-le-cas-Juazeiro.pdf
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Bahia; Juazeiro; Familiar farms; Diagnosis; Production; Agricultura Familiar; Diagnostico; Produção; Brazil.
Ano: 2001
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/134415

Diagnóstico dos sistemas de produção dos membros da Cooperativa Agropecuária Mista de Nova União.
Provedor de dados: 14
Autores: MONTEIRO, R. P.; TOWNSEND, C. R.; OLIVEIRA, V. B. V.; FERNANDES, S. R.; GONZAGA, D. S. de O. M.
A realização do diagnóstico teve por objetivo conhecer os sistemas de produção, tipificar os produtores cooperados da COOPAMNU; identificar problemas; e estreitar as relações da Embrapa com a cooperativa e os técnicos que atuam na região. As atividades do subprojeto em questão, iniciaram-se no final de 1996 com a obtenção de dados secundarios sobre os polos agropecuarios do estado e a escolha da região para as atividades de intervenção. Posteriormente foram coletados mais dados enfocando a região selecionada (Ouro Preto do Oeste, região central do estado e sua maior bacia leiteira) dando enfase aos dados referentes as organizações sociais rurais. Em abril de 1997 foram realizadas reuniões com orgãos locais de assistencia tecnica e fomento, visitas aos...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Diagnosis; Rondônia; Amazônia Ocidental; Western Amazon.; Pequeno Produtor; Cooperativa de Produtores; Sistema de Produção; Diagnostico.; Small businesses; Production technology.; Small-scale farming.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/700854

Metodologia do teste de imunofluorescência indireta para o diagnóstico da tripanosomose eqüina.
Provedor de dados: 14
Autores: SILVA, R. A. M. S.; SANCHEZ, V.; DÁVILA, A. M. R.
A tripanosomose causada pelo Trypanosoma evansi tem uma distribuição geográfica extremamente ampla. Ela ocorre no norte da África, Índia, Malásia, Indonésia, China, Rússia, Filipinas, América Central e América do Sul. O T. evansi causa a tripanosomose equina também conhecida no nordeste da Argentina e Pantanal como "mal de cadeiras". O T. evansi infecta uma ampla variedade de mamíferos e no Pantanal ele tem sido encontrado em cavalos, quoatis (Nasua nasua), cães, capivaras (Hydrochaeris hydrochaeris) e pequenos roedores (Oryzomys sp.). No Pantanal tripanosomose eqüina causa centenas de mortes todo ano. Esta publicação tem como objetivo descrever a metodologia do TIFI utilizada no Laboratório de Sanidade Animal da Embrapa Pantanal.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Tripanosomose eqüina; Teste de Imunofluorescência Indireta; Trypanosomosis; Diagnosis; Method; Animal sanity.; Diagnostico; Método; Sanidade Animal.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/811012

Diagnóstico da pecuária em Rondônia.
Provedor de dados: 14
Autores: COSTA, N. de L.; MAGALHÃES, J. A.; TAVARES, A. C.; TOWNSEND, C. R.; PEREIRA, R. G. de A.; SILVA NETTO, F. G. da.
Aspectos gerais sobre Rondônia: tipos de exploração; estrutura fundiária; infra-estrutura de apoio a produção; pesquisa e assistência técnica; rebanho bovino; Pecuária de leite: produção estadual, industrialização, oferta e demanda de leite do estado, regições produtoras, prognóstico; Pecuária de corte: constituição do rebanho de corte, abates e produção de carne, comercialização do gado, matadouros, frigoríficos, regões de pecuária de corte; Bufalos: rebanho, estrutura das propriedades, comercialização e prognóstico.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Diagnosis; Rondônia; Amazônia Ocidental; Western Amazon.; Pecuária; Diagnostico.; Livestock.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/698961

Brucelose e tuberculose bovina: epidemiologia, controle e diagnóstico.
Provedor de dados: 14
Autores: ALMEIDA, R. F. C. de; SOARES, C. O.; ARAÚJO, F. R. de.
Cap. 1: Brucelose bovina: epidemiologia e controle. Cap. 2: Brucelose bovina: diagnóstico. Cap. 3: Tuberculose bovina: epidemiologia e controle. Cap. 4: Tiberculose bovina: diagnóstico.
Tipo: Livro técnico (INFOTECA-E)
Palavras-chave: Controle; Diagnosis; Control.; Bovino; Brucelose; Diagnostico; Doença Animal; Epidemiologia; Sanidade Animal; Tuberculose.; Animal health; Animal diseases; Epidemiology; Brucellosis; Cattle; Tuberculosis.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/325789

Coleção de Microrganismos Patogênicos a Caprinos e Ovinos - CMPCO.
Provedor de dados: 14
Autores: FACCIOLI-MARTINS, P. Y.; SOUZA, V. de; PINHEIRO, R. R.
Resumo: Esta publicação tem por objetivo descrever a história, a composição da Coleção de trabalho de Microrganismos Patogênicos a Caprinos e Ovinos, e de que forma ela tem conservado, caracterizado, documentado e utilizado seus isolados. A coleção foi criada em 2012 e atualmente é composta por 141 cepas bacterianas e seis cepas virais, pertencentes às espécies Corynebacterium pseudotuberculosis, Staphylococcus aureus e ao gênero Lentivirus. Esses isolados são utilizados para produção de antígenos para imunodiagnósticos, em diagnósticos moleculares e na avaliação de resistência a antimicrobianos e ação de anti-sépticos e antimicrobianos. Participa de projetos das coleções institucionais e de gestão da qualidade. [Collection of Goat and Sheep Pathogenic...
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Caracterização; Germplasm bank; Characterization; Diagnosis; Quality; Pathogenic Microorganisms; Caprino; Ovino; Banco de Germoplasma; Conservação; Resistência Genética; Diagnostico; Qualidade; Sheep diseases; Goat diseases; Germplasm conservation; Conservation banks; Genetic resistance; Small ruminants; Brazil.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1123793

Procedimentos para coleta e remessa de sementes e plantas para o diagnostico de doencas em laboratorios de fitopatologia.
Provedor de dados: 14
Autores: NASSER, L. C. B.
bitstream/item/121719/1/gtec-44.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Plant diseases; Seed; Diagnosis; Coleta; Diseases; Fitopatologia; Plants; Procedimento.; Diagnostico; Doença de Planta; Doença; Planta; Semente.
Ano: 1999
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/545885

Pecuária de leite em Rondônia, seus problemas e soluções.
Provedor de dados: 14
Autores: OLIVEIRA, J. N. S.; GONÇALVES, C. A.; OLIVEIRA, J. R. da C.; SILVA NETTO, F. G. da.
Identifica problemas tecnologicos, politicos e administrativos que afetam o desenvolvimento da pecuaria leiteira em Rondônia.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Diagnosis; Reunião técnica; Technical meeting; Porto Velho (RO); Rondônia; Amazônia Ocidental; Western Amazon; Gado Leiteiro; Produção Leiteira; Diagnostico; Dairy cattle; Milk production.
Ano: 1983
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/698079

Envenenamento botrópico em bovinos: tratamento opcional.
Provedor de dados: 14
Autores: NOVAES, A. P. de; LUCAS, S.; ABE, A. S.; FERNANDES, W.; PUORTO, G.; ALMEIDA, I. L. de.
Envenenamento botropico em bovinos. Envenenamento por B. alternatus, B. neuwiedi, B. moojeni, B. jararacussu, B. jararaca, B.atrox.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Bovinos; Envenenamento; Bovine; Diagnosis; Treatment.; Intoxicação animal; Diagnostico; Tratamento.; Poisoning; Dietary exposure.
Ano: 1986
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/42739

Diagnóstico da mastite bovina.
Provedor de dados: 14
Autores: OLIVEIRA, V. M. de.
Sintomas da mastite; Clinica; Subclinica; Celulas somaticas - definicao; Diagnostico da mastite clinica; Exame do ubere; Exame visual (antes da ordenha); Exame manual do ubere e tetas (apos a ordenha); Teste da caneca de fundo escuro ou caneca telada; Diagnostico diferencial da mastite; Lesoes das tetas; Diagnostico da mastite subclinica; Metodos de deteccao das celulas somaticas; CCS atraves de contadores eletronicos; Contagem direta em laminas (CDL); California Mastitis Test (CMT); Teste do Viscosimetro (WMT); Teste para medir a condutividade eletrica; Teste com o "Detector de Condutividade" manual; Sistema de condutividade acoplado a ordenhadeira mecanica; Como calcular o nivel de mastite em um rebanho; Mastite clinica; Mastite subclinica; Exames...
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Bovine; Bovinos; Control; Controle; Diagnosis; Disease; Enfermidade; Exames; Gado de leite; Mamimary gland diseases; Mastite; Mastite bovina; Symptions.; Animal; Bovino; Diagnostico; Doença; Doença Animal; Gado Leiteiro; Infecção; Mamite; Profilaxia; Sintoma.; Animal diseases; Cattle; Dairy cattle; Mastitis.; Infection.
Ano: 1998
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/957987

Cigarrinhas-das-pastagens em Rondônia: diagnóstico e medidas de controle.
Provedor de dados: 14
Autores: TOWNSEND, C. R.; TEIXEIRA, C. A. D.; SILVA NETTO, F. G. da; PEREIRA, R. G. de A.; COSTA, N. de L.
Este trabalho teve por objetivo diagnosticar a abrangência e o nível de ocorrência de cigarrinhas-das-pastagens em Rondônia, bem como propor medidas de controle, com base em resultados de pesquisas.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Diagnosis; Rondônia; Amazônia Ocidental; Western Amazon; Pastagem; Praga de Planta; Cigarrinha das Pastagens; Deois Flavopicta; Diagnostico; Pastures; Plant pests.
Ano: 2001
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/703813

Diagnóstico tecnológico dos pequenos produtores de dendê no Estado do Pará.
Provedor de dados: 14
Autores: MULLER, A. A.; COUTINHO FILHO, D.; SOUZA, L. A. de; SILVA, H. M. e; VIEGAS, I. de J. M.; CELESTINO FILHO, P.; CARVALHO, R. de A.
bitstream/item/57887/1/Belem-CirTec6.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Pequeno agricultor; Plantação; Pará; Brasil; Oil palm; Diagnosis; Production; Plantation; Fertilizer; Yield.; Adubação; Dendê; Elaeis Guineensis; Diagnostico; Produtividade; Produção.; Amazonia; Small farms.
Ano: 1990
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/383723

Acaro da necrose do coqueiro - Aceria guerreronis (Keifer): aspectos bioecologicos, sintomas, danos e medidas de controle.
Provedor de dados: 14
Autores: ALENCAR, J. A. de; HAJI, F. N. P.; BARBOSA, F. R.
Trata sobre a necrose do coqueiro: aspectos taxonomicos e bioecologicos, distribuicao geografica, sintomas e danos, nivel de dano economico, medidas de controle- controle cultural, controle biologico e controle quimico.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Acaro da necrose; Controle; Cocos; Diagnosis; Damage; Control; Coconut; Disease.; Coco; Doença; Aceria guerreronis; Cocos Nucifera; Dano; Ecologia; Sintoma.; Ecology.
Ano: 1999
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131429

Diagnóstico ambiental expedito do Parque Cambuí, Campo Largo-PR, aplicado à educação ambiental.
Provedor de dados: 14
Autores: RACHWAL, M. F. G.; SOUZA, R. G.; WHITERS, L. H. O.; MULLER, C. R. C.; FADANELLI, K.; SCHIAVON, G.
bitstream/item/17084/1/doc74.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Paraná; Public parks; Diagnosis; Bioma; Conservação da natureza.; Educação Ambiental; Diagnostico; Parque.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/307058

Diagnóstico do desenvolvimento da heveicultura no estado de Rondônia.
Provedor de dados: 14
Autores: OLIVEIRA, J. N. S.; MEDRADO, M. J. S.
Faz um diagnostico da cultura da seringueira em Rondonia, identificando problemas que afetam a produtividade fisica e economica da cultura no estado.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Rubber tree; Diagnosis; Reunião técnica; Technical meeting; Ouro Preto d'Oeste (RO); Ariquemes (RO); Rondônia; Amazônia Ocidental; Western Amazon; Heveicultura; Seringueira; Hevea Brasiliensis; Desenvolvimento Econômico; Diagnostico; Economic development.
Ano: 1983
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/698080

Diagnóstico da pecuária bovina no estado do Pará.
Provedor de dados: 14
Autores: SIMAO NETO, M.; GONCALVES, C. A.; SERRÃO, E. A. S.; FREIRE, E. M. da S.; CARVALHO, J. dos S.; VEIGA, J. B. D.; TEIXEIRA NETO, J. F.; DUTRA, S.
bitstream/item/199002/1/Diagnostico-da-pecuaria.pdf
Tipo: Outras publicações técnicas (INFOTECA-E)
Palavras-chave: Pará; Brasil; Diagnosis; Bovinocultura; Diagnostico; Amazonia; Cattle breeding.
Ano: 1988
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/387675

Diagnóstico da pesca e aspectos da biologia reprodutiva dos peixes da bacia hidrográfica do rio Taquari, Mato Grosso do Sul, Brasil.
Provedor de dados: 14
Autores: RESENDE, E. K. de; SANTOS, D. C. dos.
Neste trabalho são apresentados os primeiros resultados, dentro de uma visão integrada, dos aspectos biológicos e da atividade pesqueira na bacia hidrográfica do rio Taquari, trazendo informações que contribuam para esclarecer a problemática da pesca na bacia. - Produção pesqueira do rio Taquari; Análise dos guias de controle de pescado e autos de infração; Missões de fiscalização relâmpago; Cálculos para estimativa da produção pesqueira clandestina (PPC); Produção pesqueira obtida do SCPESCA/MS de 1997; Produção pesqueira estimada de 1997 (PPAE) = PPC + PP.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Biologia reprodutiva; Rio Taquari; Fishery; Diagnosis; Reproductive biology.; Diagnostico; Peixe; Pesca.; Pantanal; Fish.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/810510

Identificação de problemas que afetam as culturas anuais em Rondônia.
Provedor de dados: 14
Autores: OLIVEIRA, J. N. S.
Apresenta problemas que caracterizam as culturas anuais em Rondônia, discutidos no Encontro realizado em abril de 1982, envolvendo tecnicos, assessores, extensionistas e pesquisadores. Foram expostos Programas de Pesquisas de arroz de sequeiro, milho e feijao em andamento em Rondonia. Faz-se um diagnostico destas culturas estabelecendo prioridades futuras para a execucao de projetos. Propoe solucoes, identifica mecanismos de Difusao de Tecnologias que integram pesquisadores, extensionistas e produtores, a fim de agilizar o processo de Transferencia de Tecnologias.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Reunião técnica; Technical meeting; Diagnosis; Ouro Preto d'Oeste (RO); Rondônia; Amazônia Ocidental; Western Amazon; Arroz; Oryza Sativa; Feijão; Phaseolus Vulgaris; Milho; Zea Mays; Cultivo Anual; Diagnostico; Pesquisa Agrícola; Rice; Beans; Corn; Annuals; Agricultural research.
Ano: 1983
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/698081

Manual para laboratório de fitopatologia.
Provedor de dados: 14
Autores: FERNANDEZ, M. R.
Objetivos da fitopatologia. Manutenção básica do laboratório. Segurança no laboratório. Esterilização - exigência básica para o trabalho com microorganismos. Estabelecimento de condições assépticas. Métodos de esterilização. Tipos de meios de cultura. Preparo de meios de cultura. Preparo de culturas de fungos e de bactérias. Condições para crescimento de patógenos. Fungos. Bactérias. Incubadoras. Isolamento de fungos e de bactérias de tecido vegetal infectado. Aspectos gerais. Métodos de isolamento. Isolamento de patógenos do solo. Descontaminação de culturas. Aspectos gerais. Métodos mecânicos. Métodos químicos. Ácaros. Isolamento monospórico. Postulados de Koch ou regras de prova da patogenicidade. Técnicas para a inoculação de plantas. Preparo do...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Diagnosis; Plant disease; Técnica de laboratório.
Ano: 1993
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/815639

Teste sorológico de imunofluorescência indireta para o diagnóstico da neosporose em bovinos.
Provedor de dados: 14
Autores: ANDREOTTI, R.; MATTOS, M. de F. C.; OLIVEIRA, J. M. de; LOCATELLI-DITTRICH, R.
Diagnóstico da neosporose. Métodos de diagnóstico sorológico. Reação de imunofluorescência indireta para Neospora caninum.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Diagnosis; Immunofluorescence.; Bovino; Elisa; Diagnostico; Imunofluorescência; Neosporose; Neospora Caninum; Sanidade Animal.; Animal health; Cattle; Neosporosis.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/324145

Diagnóstico e controle da neosporose em bovinos.
Provedor de dados: 14
Autores: ANDREOTTI, R.; LOCATELLI-DITTRICH, R; SOCCOL, V. T.; PAIVA, F.
A neosporose em bovinos é uma doença causada pelo protozoário Neospora caninum, identificado pela primeira vez em 1988, em cães com encefalomielite, o hospedeiro definitivo. Estudos sorológicos de vários países demonstraram o parasita como a maior causa de abortos em rebanhos leiteiros. A doença está amplamente disseminada nos diferentes continentes. A importância econômica da neosporose bovina é atribuida principalmente aos custos associados ao aborto, ao valor dos fetos, à inseminação artificial ou à cobertura, à diminuição da produção de leite, ao aumento do descarte e à reposição dos animais.Em bovinos, os dois mecanismos de infecção por N. caninum são as transferência do parasita da mãe para o feto (transmissão vertical ou infecção congênita) e a...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Controle; Diseases; Biology; Diagnosis; Control.; Biologia; Bovino; Cão; Diagnostico; Doença; Neosporose; Neospora Caninum; Sanidade Animal.; Animal health; Dogs; Cattle; Neosporosis.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/324070

Atualização sobre tricomonose genital bovina.
Provedor de dados: 14
Autores: PELLEGRIN, A. O.; LEITE, R. C.
A tricomonose é uma doença venérea de bovinos causada por um protozoário flagelado denominado Tritrichomonas foetus (T.foetus), cujo habitat é o trato genital de bovinos sendo transmitido do macho para a fêmea através da monta ou pelo uso de sêmen contaminado. O T.foetus é um protozoário de morfologia piriforme com três flagelos em posição cranial e um caudal, cujo tamanho oscila entre entre 8-18mm de comprimento e 4-9 de largura. Se caracteriza por possuir 4 flagelos, 3 deles são anteriores e livres e o 4o acompanha a membrana ondulante, que é perfeitamente visível quando em microscopia, localizando-se na parte posterior.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Tricomonose genital bovina; Patogenia; Controle; Imunoprofilaxia; Bovine; Disease; Pathogeny; Diagnosis; Control.; Bovino; Diagnostico; Doença; Epidemiologia; Imunidade; Tratamento.; Epidemiology; Immunity.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/811109

Métodos de diagnósticos parasitológicos das tripanosomoses bovinas e eqüinas.
Provedor de dados: 14
Autores: SILVA, R. A. M. S.; SANCHEZ, V.; DÁVILA, A. M. R.
As hemoparasitoses constituem enfermidades amplamente distribuídas em toda a América Latina e Caribe causando efeitos negativos na saúde dos rebanhos animais e principalmente sobre a produção e rentabilidade dos sistemas de produção animal estabelecidos nas diferentes regiões do continente (Tamasaukas, 2000). Dentre os hemopar·sitos que causam importantes enfermidades nos animais domésticos e silvestres na América do Sul, destacam-se os Trypanosoma evansi e T. vivax. No Pantanal o T. evansi causa tripanosomose eqüina também conhecida como "mal de cadeira". É uma das doenças causadas por protozoários mais importantes do Pantanal. O objetivo deste trabalho foi descrever os métodos parasitológicos utilizados no Laboratório de Sanidade Animal da Embrapa...
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Método parasitologico; Tripanosomose; Parasitological method; Diagnosis; Trypanosomosis.; Diagnostico.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/811014

Use of molecular tools for mollusc disease diagnosis
Provedor de dados: 5
Autores: Berthe, Franck; Burreson, Eugene; Hine, Mike.
Recently, an increasing number of research teams are engaged in developing DNA-based diagnostic techniques for mollusc pathogens. These techniques are slowly moving from development in specialised laboratories for research purposes, to routine application and are expected to find an increasing use in routine disease monitoring programs in mollusc aquaculture and in efforts to prevent the spread of pathogens within and between nations. Considering the probable development and interest in these diagnostic techniques, the potential for wide applications in aquaculture and the inherent problems currently associated with their use, it appears necessary to address these issues.
Tipo: Text
Palavras-chave: Molecular tools; Mollusc disease; Diagnosis; Pathology.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-3291.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - Final Report 4th January 1999 to 3rd January 2002
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf

Permanent Advisory Network for Diseases in Aquaculture (PANDA)
Provedor de dados: 5
Autores: Arzul, Isabelle; Ariel, E.; Hill, B.
The PANDA project aims to establish a permanent network of aquatic animal health specialists (researchers and diagnosticians) to provide a forum for the debate of issues concerning diseases in European aquaculture, and to communicate the results of these discussions to the European Commission with provision of advice on EU policy and legislation for aquatic animal health. PANDA consists of five scientific work packages: risk analysis, epidemiology, diagnosis, environmentally safe strategies for disease control, training in research and diagnostics. These work packages are lead by PANDA consortium members, supported by small task force of specialists, with wider input from experts registered in the PANDA network. Focused workshops and ongoing online...
Tipo: Text
Palavras-chave: Aquatic infectious diseses; Diagnosis; Risk analysis; PANDA; Animal health.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3320.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and...
Tipo: Text
Palavras-chave: Pathology; Diagnosis; Oyster; Herpes like virus; Herpesviridae; Immunology; Detection; Virus; Bivalves.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15523/12910.pdf

Project ROMEO : E. C. n° FA-S2 9052 - Research objectives : Mortality in European Oysters - Final Report
Provedor de dados: 5
Autores: Renault, Tristan; Arzul, Isabelle.
Crassostrea gigas is increasingly being cultured in a great number of countries from animaIs originally introduced from Japan, and is one of the most exploited bivalve species in the EU (FAO, 1989). Although no serious pathogen was detected previously in Japanese oysters in Europe, mortalities have been observed since the introduction of this bivalve species into the EU. Thus, oyster cultivation may be endangered by the occurrence of epizootics, especially virus diseases, which are considered one of the maj or risks to production. Indeed, mortalities have been described among different species of ostreids and are associated with the presence of viruses belonging to various families. The first description of a virus was reported in adult Eastern oyters,...
Tipo: Text
Palavras-chave: Pathology; Virology; Herpes-like infection; Detection; Diagnosis; Herpesviridae; Oyster; Crassostrea gigas; Mortality.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15536/12915.pdf

Contribution à l'étude de mollusques marins : Iridovirus-like et Herpèsvirus-like. Description, caractérisation biochimique, cycle de multiplication viral, diagnostic et étude épidémiologique
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Among the marine molluscs, Pacifie oyster, Crassostrea gigas, is actually the most reared specie in the world. Noticely in France, where it was imported between 1960 and 1970, and replaced by Portuguese oyster, Crassostrea angulata, which specie was decimated during this period because of iridoviruses. Uncontroled introductions of new species may however be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases of bivalve molluscs in importing country. In addition to these observations, the quasi monobreeding of Pacific oyster in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this specie, namely the diagnosis of viral diseases. Several families of viruses...
Tipo: Text
Palavras-chave: Mollusques; Crassostrea gigas; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Molluscs; Crassostrea gigas; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00041/15230/12548.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" Third Periodic Progress Report : 4th January 2001 to 3rd January 2002
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Herpesvirus infections have been reported in different bivalves species around the world. Specific diagnostic tools are needed in order ta assess the causative raIe of these agents in mortalities observed among bivalves There is currently a lack of information concerning the occurrence of bivalve herpesviruses. This is probably due ta the lack of suitable diagnostic tools. The basic method for examination of bivalve samples is predominantly histology. This enables the identification of any cellular changes, but is not conclusive for virus identification. Transmission electron microscopy is a necessity for visual confirmation of viral infections. However, histology and electron microscopy are time consuming and inadequate for epidemiological studies. The...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Virus; Oyster; Detection; OsHV-1; Herpesviridae.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00044/15524/12911.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular immunological and cellular tools "VINO". Second periodic progress report: 4th January 2000 at 3rd January 2001
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using teclmiques developed by the patiners to characterise viruses by studying their genome and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpesvirus (OsHV-1) DNA sequence with determination of the genome structure. 2 - Comparing OsHV -1 with viruses belonging to the Herpesviridae family on the basis of sequence data and genome structure. 3 -...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00048/15947/13377.pdf

Contribution à l'étude de virus de mollusques marins apparentés aux Iridoviridae et aux Herpesviridae
Provedor de dados: 5
Autores: Le Deuff, Rose-marie.
Among the marine molluscs, Pacific oyster, Crassostrea gigas, is actually the most reared species in the world. Noticeably in France, where it was imported between 1960 and 1970, and replaced Portuguese oyster, Crassostrea angulata. Intensification of breeding and the associated introductions of new species may be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases in bivalve molluscs. In addition to these observations, the quasi monobreeding of C. gigas in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this species, namely the diagnosis of viral diseases. Several families of viruses have been described within marine bivalves molluscs. The purpose of this...
Tipo: Text
Palavras-chave: Mollusques bivalves; Crassostrea gigas; Huître japonaise; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Bivalve molluscs; Crassostrea gigas; Pacific oyster; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00107/21792/19367.pdf

Vibrio nigripulchritudo monitoring and strain dynamics in shrimp pond sediments
Provedor de dados: 5
Autores: Walling, Emilie; Vourey, Elodie; Ansquer, Dominique; Beliaeff, Benoit; Goarant, C.
Aims: A description of bacterial pathogens in shrimp ponds is necessary to understand their pathogenesis. Vibrio nigripulchritudo was shown to contain saprophytic and pathogenic strains among New Caledonian isolates. We established a method to map the development of V. nigripulchritudo in pond sediments at three different genetic levels: the species level, then at the pathogenic cluster level and finally at the plasmid level, present only in all highly pathogenic isolates. Methods and Results: PCR methods were applied to shrimp pond sediments both before and after a mortality outbreak. Using crude samples, the species V. nigripulchritudo is not detected at first (0/42 samples at day 56 post stocking) but appears frequently in the sediments after the...
Tipo: Text
Palavras-chave: Detection; Diagnosis; Diseases; Ecology; PCR; Plasmids; Soil; Virulence.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00004/11484/8177.pdf

Marteiliosis in molluscs: A review
Provedor de dados: 5
Autores: Berthe, Franck; Leroux, Frederique; Adlard, Robert; Figueras, Antonio.
Among mollusc pathogens, paramyxeans are an important group of protistan parasites belonging to the genera Marteilia and Marteilioides. Martelia refringens and M. sydneyi are of particular concern given their potential impact on mollusc aquaculture world-wide. Aber disease and QX disease are currently listed by the OIE, the World Organisation for Animal Health. After more than thirty years of existence in the scientific literature, these organisms still pose questions and research challenges to investigators. This paper reviews current knowledge of the group and key references. The review was focused on taxonomy, epidemiology, pathology and potential control methods for these organisms in order to enhance understanding of paramyxean infection issues in...
Tipo: Text
Palavras-chave: Paramyxean parasites; Diagnosis; Pathology; Epidemiology; Taxonomy.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-406.pdf

Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maxiumus (Bivalvia): application to indirect immunofluorescence diagnosis
Provedor de dados: 5
Autores: Le Gall, Gyslaine; Mourton, Chantal; Boulo, Viviane; Paolucci, Francis; Pau, Bernard; Mialhe, Eric.
Using hybridoma technology, monoclonal antibodies (MABs) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten maximus. The 5D7 MAB was then selected as a specific reagent suitable for an indirect immunofluorescence assay (IIFA) and evaluated for RLO diagnosis on P. maxiumus gill smears. Monoclonal antibody 5D7 showed no cross-reactivity with an RLO associated with a related pectinid species Chlamys opercularis
Tipo: Text
Palavras-chave: Diagnosis; Rickettsiales like organism; Monoclona antibody; Pecten maximus.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/publication-2762.pdf

Genomics and mollusc pathogens: trends and perspective
Provedor de dados: 5
Autores: Renault, Tristan.
Global mollusc production is continuously increasing and reached 12.8 million tonnes in 2004 representing 28.2 percent of the total world production valued at US$ 9.4 billions. However, cultivated molluscs may suffer from severe mortality outbreaks. Among the possible causes is the occurrence of infectious diseases due to a variety of pathogens including protozoan parasites, bacteria and viruses. Preventing and controlling diseases has become a priority for aquaculture sustainability. However, molluscs are unique in terms of health management. Molluscs like other invertebrates, lack a true adaptative immune system. Vaccination can not be used to protect them against pathogens. Moreover, molluscs are usually reared in the open sea which strongly limits the...
Tipo: Text
Palavras-chave: Diagnosis; Phylogeny; Genomics; Mollluscs; Pathogens.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4574.pdf

Diagnosis of herpes-like virus infections in oysters using molecular techniques
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile.
Several herpes-like virus infections have been reported since 1991 among different bivalve species around the world (Nicolas et al., 1992; Comps and Cochennec, 1993; Renault et al., 1994; Hine and Thome, 1997; Hine 1997). To date, detection of these infections has been strongly correlated with severe losses of oyster larvae and spat. The virus infections are currently diagnosed by laborious histologicai and transmission electron microscopical examinations. These techniques, however, facilitate neither rapid diagnosis nor the ability to analyse large sample numbers.
Tipo: Text
Palavras-chave: Diagnosis; Ostrea edulis; Crassostrea gigas; Herpes like virus.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/acte-2902.pdf

Instructions for Using the MInCir Scale to Assess Methodological Quality in Diagnostic Accuracy Studies
Provedor de dados: 77
Autores: Manterola,Carlos; Cartes-Velasquez,Ricardo; Otzen,Tamara.
Globally, the assessment of methodological quality (MQ) in biomedical research is an area of dynamic development over recent years. Understood as a complex and multidimensional construct, various groups have proposed tools for its assessment. Our team has designed and validated scales to assess MQ of therapy and prognosis studies. However, as with other instruments, it is necessary to specify in detail how it is applied, in such a way as to be able to standardize the measurements made with this instrument. A detailed description is presented of the 3 domains (type of research design [single item domain], size of population studied [single item domain], and methodology 7 items) and 9 items comprising the scale, specifying for each item how to assess the...
Tipo: Journal article
Palavras-chave: Investigative Techniques; Methods/epidemiology; Methodological studies; Diagnosis; Diagnostic tests; Reproducibility of Results; Evaluation Studies as Topic; Epidemiological Studies; Evidence-Based Medicine.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100012

Minor Salivary Glands Morphology in Xerostomia Patients
Provedor de dados: 77
Autores: Liquidato,Bianca Maria; Barros,Mirna Duarte; Filho,Ivo Bussoloti; Pereira,Celina Siqueira Barbosa; Macéa,Maria Inêz Marcondes.
The minor salivary glands are found scattered throughout the oral mucosa, especially in the lips and soft palate mucosa. Several factors can cause xerostomia, whereas the salivary glands histological characteristics are also considered as factors for defining the etiology. Thus, the minor salivary glands biopsy represents an essential tool for attending the required diagnosis criterion in the classification of Sjögren's Syndrome patients, since it does not present risk for the patient. The objective of this study is to determine the histological description of the minor salivary glands obtained from the biopsies of xerostomia patients and to classify the minor salivary gland histological aspect as the Sjögren's Syndrome. Forty laminas of xerostomia...
Tipo: Journal article
Palavras-chave: Sjögren's syndrome; Xerostomia; Salivary glands; Saliva; Diagnosis.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400031

Comparison of different diagnostic tests in dogs uninfected and naturally infected with visceral leishmaniasis
Provedor de dados: 80
Autores: Sassaki,CY; Colodel,MM; Ferreira,I; Nogueira,FS; Lucheis,SB; Langoni,H; Rocha,NS.
Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofluorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more effective than PCR of lymph node aspirate culture. There was no statistical difference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Dogs; Visceral leishmaniasis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300015

Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
Provedor de dados: 80
Autores: Camargo,JB; Langoni,H; Troncarelli,MZ; Machado,JG; Lucheis,SB; Padovani,CR.
Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Dog; Diagnosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300007

Canine visceral leishmaniasis in the Northeast Region of Brazil
Provedor de dados: 80
Autores: Brito,Flávio Gonçalves; Langoni,Helio; Silva,Rodrigo Costa da; Rotondano,Tereza Emmanuelle de Farias; Melo,Marcia Almeida de; Paz,Giselle Souza da.
Abstract  Background  Visceral leishmaniasis (VL) is a zoonosis that affects dogs and other mammals, including humans. Contact with dogs is a major risk factor for humans. This disease is endemic in several regions of Brazil. The aim of this study was to determine the prevalence of Leishmania spp. infection in dogs and to correlate it with possible risk factors.  Methods  Blood samples were collected from 391 dogs of different ages, breeds, and both genders, coming from Campina Grande, Paraíba state, Brazil. An epidemiological questionnaire was employed in order to identify risk factors associated with the disease. Serological tests were performed using indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA S7®) and polymerase chain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kala-azar; Diagnosis; Dog; Zoonosis; Risk factors.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100311

Molecular techniques for the study and diagnosis of parasite infection
Provedor de dados: 80
Autores: Tavares,RG; Staggemeier,R; Borges,ALP; Rodrigues,MT; Castelan,LA; Vasconcelos,J; Anschau,ME; Spalding,SM.
In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parasite infection; Diagnosis; Molecular techniques; Molecular epidemiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300003

Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction
Provedor de dados: 80
Autores: Braga,Audrey Rennó Campos; Langoni,Hélio; Lucheis,Simone Baldini.
Background: This study aimed to evaluate the occurrence of Leishmaniaspp. in dogs and cats from Botucatu, São Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Results: Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Cat; Diagnosis; Leishmaniasis; Zoonosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200323

Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
Provedor de dados: 80
Autores: Gonçalves-de-Albuquerque,Suênia da Cunha; Silva,Rômulo Pessoa e; Morais,Rayana Carla Silva de; Trajano-Silva,Lays Adrianne Mendonça; Régis-da-Silva,Carlos Gustavo; Brandão Filho,Sinval Pinto; Paiva-Cavalcanti,Milena de.
Background : Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasite’s DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasite’s DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.Results : Two primer pairs were designed to detect the plasmid pUC18 and a triplex PCR assay targeting theLeishmania braziliensiskinetoplast DNA, the external control and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extraction control; Multiplex PCR; PUC18; Leishmaniasis; Diagnosis; False-negative result.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200326

Application of the mammalian glyceraldehyde-3-phosphate dehydrogenase gene for sample quality control in multiplex PCR for diagnosis of leishmaniasis
Provedor de dados: 80
Autores: Gonçalves,SC; Régis-da-Silva,CG; Brito,MEFC; Brandão-Filho,SP; Paiva-Cavalcanti,M.
Leishmaniasis is a neglected disease endemic in five continents. It is a severe disease that may lead to death, and its early detection is important to avoid severe damage to affected individuals. Molecular methods to detect Leishmania are considered alternatives to overcome the limitations presented by conventional methods. The aim of this study was to develop multiplex PCR systems able to detect small amounts of target DNA of Leishmania infantum and Leishmania braziliensis, and the gene coding for glyceraldehyde-3-phosphate dehydrogenase (G3PD) in mammals, enabling quality evaluation of the sample simultaneously with detection of the specific target. The systems created for G3PD recognition were combined with detection systems for L. infantum and L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Diagnosis; Glyceraldehyde-3-phosphate dehydrogenase; Quality control.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200009

Diagnosis of Giardia infections by PCR-based methods in children of an endemic area
Provedor de dados: 80
Autores: David,EB; Coradi,ST; Oliveira-Sequeira,TCG; Ribolla,PEM; Katagiri,S; Guimarães,S.
The present study was designed to estimate the prevalence of Giardia infection in preschool- and school-aged children living in an endemic area. Fecal samples from 573 children were processed by zinc sulfate centrifugal flotation, centrifugal sedimentation (using a commercial device for fecal concentration - TF-Test kit®) and polymerase chain reaction (PCR)-based methods. Of the stool samples assessed, 277 (48.3%) were positive for intestinal parasites and/or commensal protozoa. Centrifugal flotation presented the highest diagnostic sensitivity for Giardia infections. The kappa index revealed that both coproparasitological techniques closely agreed on the Giardia diagnosis (86%) versus satisfactory (72%) and poor (35%) concordances for commensal protozoan...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giardia duodenalis; Children; Diagnosis; PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200012

Ovine leptospirosis in Brazil
Provedor de dados: 80
Autores: Lucheis,SB; Ferreira Jr.,RS.
Leptospirosis is a zoonosis distributed worldwide, endemic mainly in humid subtropical and tropical countries, with epidemic potential. It affects a range of both wild and domestic animals, including sheep, which transport leptospires in their urine and, therefore, can infect other animals and humans who deal with them. Therefore, leptospirosis is characterized as an occupational zoonosis. In individual herds leptospirosis can cause severe economic loss due to miscarriages and outbreaks of mastitis with a significant reduction of milk production. The disease is caused by Leptospira interrogans, which was reclassified into 13 pathogenic species, and distributed into more than 260 serovars classified into 23 serogroups. The clinical signs of infection may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Leptospira; Diagnosis; Animal diseases.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400006

American visceral Leishmaniasis: a case report
Provedor de dados: 80
Autores: Langoni,H.; Lucheis,S. B.; Da Silva,R. C.; Castro,A. P. B.; Paes,A. C.
Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Zoonosis; Dog; Diagnosis; Hemoculture; Serology; Polymerase chain reaction (PCR).
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300012

Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review
Provedor de dados: 80
Autores: Paiva-Cavalcanti,M; Regis-da-Silva,CG; Gomes,YM.
In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Molecular diagnosis; Visceral leishmaniasis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400004

Morphology and DNA sequence data reveal the presence of Globodera ellingtonae in the Andean region
Provedor de dados: 16
Autores: Lax, P.; Rondan Dueñas, J.C.; Franco-Ponce, J.; Gardenal, C.N.; Doucet, M.E.
Potato cyst nematodes, G. rostochiensis and G. pallida, are the most economically important nematode pests of potatoes worldwide and are subject to strict quarantine regulations in many countries. Globodera ellingtonae was recently described from Oregon (USA), with its host-plant in the field being still unknown. Roots of Andean potatoes from the North of Argentina have been found attacked by this nematode, providing further evidence that this is a potato cyst nematode species, along with G. pallida and G. rostochiensis. New information about morphological, biological and molecular aspects of G. ellingtonae is provided for diagnostic purposes. The Argentine population showed morphological differences from specimens from Oregon; therefore, new diagnostic...
Tipo: Article / Letter to the editor
Palavras-chave: Andean potato; Argentina; Diagnosis; Hsp90 gene; Morphology; Potato cyst nematode; 42.76; 42.64.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/506790

Use of Contrast-Enhanced CT in the Diagnosis of Abscesses in Cattle
Provedor de dados: 19
Autores: LEE, Ki-Ja; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; SASAKI, Naoki; ISHII, Mitsuo; INOKUMA, Hisashi; IWASAKI, Toshiroh; MIYAKE, Yoh-Ichi; YAMADA, Kazutaka; 古林, 与志安; 松本, 高太郎; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽; 山田, 一孝.
We report here the non-contrast and contrast-enhanced computed tomography performed in two calves with brain abscess and multiple pulmonary abscesses with pharyngeal abscess, respectively. Contrast-enhanced computed tomography was useful in the diagnosis of these diseases in both calves. The diseases were confirmed by histopathological examination.
Palavras-chave: Abscess; Cattle; Contrast-enhanced CT; Diagnosis.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3891

Clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50
Provedor de dados: 19
Autores: Miyama, Takako; Inokuma, Hisashi; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; 猪熊, 壽; 玄, 学南.
The clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 was examined in dogs in an area where B. gibsoni infection was endemic. Only 8 among 14 dogs with acute type B. gibsoni infection without a previous history of infection were positive. This high percentage of false-negative results is thought to be a weak point of ELISA as a diagnostic tool. However, 14 other anemic dogs with a confirmed history of B. gibsoni infection were all positive, thus confirming the higher sensitivity of ELISA in detecting a history of infection.
Palavras-chave: Babesia gibsoni; Diagnosis; ELISA.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/925

Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay
Provedor de dados: 19
Autores: Sandagdorj, Narantsatsral; Goo, Youn-Kyoung; Terkawi, Mohamad Alaa; Soma, Takehisa; Luo, Yuzi; Li, Yan; Cao, Shinuo; Aboge, Gabriel Oluga; Nishikawa, Yoshifumi; Badgar, Battsetseg; Xuan, Xuenan.
Among the previously established enzyme-linked immunosorbent assays (ELISAs), an ELISA using the full length of recombinant thrombospondin-related adhesive protein of Babesia gibsoni (rBgTRAPf) is considered as the most sensitive diagnostic method for the detection of antibody to B. gibsoni in dogs. However, the expression of rBgTRAPf in high concentration is poor and thus limits its usefulness as diagnostic antigen. To improve its expression level, we have truncated BgTRAPf into two fragments having either N- or C-terminus (BgTRAPn or BgTRAPc). The expression of BgTRAPc protein in Escherichia coli yielded adequate recombinant protein. The specificity and sensitivity of ELISAs with the truncated proteins were determined using B. gibsoni-experimentally...
Palavras-chave: Babesia gibsoni; Diagnosis; Enzyme-linked immunosorbent assay (ELISA); Thrombospondin-related adhesive protein (TRAP).
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3115

Diagnosis Of Trypanosoma evansi Infection Among Sudanese Camels Imported To Egypt Using Card Agglutination Test (CATT) And Antigen Detection Latex Agglutination Test (Suratex)
Provedor de dados: 19
Autores: Elsaid, H. M.; Nantulya, V. M.; Hilali, M.
Palavras-chave: Trypanosoma evansi; Dromedary camel; Diagnosis; Card agglutination test; Suratex.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/298

Comparison of PCR with Parasitology and Serology in the Diagnosis of a Low Virulent Strain of Trypanosoma brucei gambiense in mice
Provedor de dados: 19
Autores: Mbati, P. A.; Hirumi, K.; Inoue, Noboru; Situakibanza, N. H.; Hirumi, Hiroyuki; 井上, 昇.
Palavras-chave: Trypanosoma brucei gambiense; Mice; Mel-B; Diagnosis; PCR.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/310

アフリカトリパノソーマ症に対する新規ワクチン法の開発および診断法の確立に関する研究
Provedor de dados: 19
Autores: Kuboki, Noritaka.
平成18年度
Palavras-chave: African trypanosoma; Vaccine; Diagnosis.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/970

Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates
Provedor de dados: 19
Autores: THEKISOE, Oriel M. M; BAZIE, Raoul S. B; CORONEL-SERVIAN, Andrea M; SUGIMOTO, Chihiro; KAWAZU, Shin-ichiro; INOUE, Noboru; 井上, 昇.
This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P&gt;0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored...
Palavras-chave: Diagnosis; DNA template; LAMP; Parasitic disease; Trypanosoma.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2691

Trypanosoma evansi Infections : Towards Penside Diagnosis
Provedor de dados: 19
Autores: Nantulya, V. M.; Diall, O.
Palavras-chave: Trypanosoma evansi; Diagnosis; Circulating antigens; Latex agglutination test (Suratex®).
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/296

Application of direct agglutination test and fast agglutination screening test for serodiagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil
Provedor de dados: 19
Autores: Silva, E. S.; Schalig, H. D. F. H.; Kroon, N. C. M.; Schoone, G. J.; Gontijo, C. M. F.; Pacheco, Raquel S.; Brazil, R. P.
We have tested an indirect fluorescence technique (IFAT), fast agglutination screening test (FAST) and direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in serum and blood samples from patients with visceral and cutaneous leishmaniasis in Brazil. For visceral leishmaniasis the results obtained with DAT showed a sensitivity and specificity of 100% with a cut-off value of 1:800. The blood and serum samples could also be clearly read in FAST using a 1:100 dilution with the same high sensitivity. Both DAT and FAST were not able to detect significant amounts of antibodies in samples from cutaneous leishmaniasis patients.
Palavras-chave: Visceral leishmaniasis; Diagnosis; DAT; FAST.
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/140

CURRENT SERODIAGNOSTIC MEASURES FOR EQUINE BABESIOSIS
Provedor de dados: 19
Autores: Hirata, Haruyuki; Yokoyama, Naoaki; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; Babesia caballi; Diagnosis; ELISA; ICT.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/638

Identification and Characterization of a Trypanosoma congolense 46 kDa Protein as a Candidate Serodiagnostic Antigen
Provedor de dados: 19
Autores: ZHOU, Mo; SUGANUMA, Keisuke; RUTTAYAPORN, Ngasaman; NGUYEN, Thu-Thuy; YAMASAKI, Shino; IGARASHI, Ikuo; KAWAZU, Shin-ichiro; SUZUKI, Yasuhiko; INOUE, Noboru; 五十嵐, 郁男; 井上, 昇.
Trypanosoma congolense is a major livestock pathogen in Africa, causing large economic losses with serious effects on animal health. Reliable serodiagnostic tests are therefore urgently needed to control T. congolense infection. In this study, we have identified one T. congolense protein as a new candidate serodiagnostic antigen. The 46.4 kDa protein (TcP46, Gene ID: TcIL3000.0.25950) is expressed 5.36 times higher in metacyclic forms than epimastigote forms. The complete nucleotide sequences of TcP46 contained an open reading frame of 1,218 bp. Southern blot analysis indicated that at least two copies of the TcP46 gene were tandemly-arranged in the T. congolense genome. The recombinant TcP46 (rTcP46) was expressed in Escherichia coli as a glutathione...
Palavras-chave: Diagnosis; ELISA; Nagana; TcP46; Trypanosoma.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3983

Babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen
Provedor de dados: 19
Autores: Zhou, Jinlin; Jia, Honglin; Nishikawa, Yoshifumi; Fujisaki, Kozo; Xuan, Xuenan.
A cDNA encoding the rhoptry-associated protein 1 (RAP-1) homologue was obtained by immunoscreening an expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 1740bp. Computer analysis suggested that the sequence contains an open reading frame of 1425bp encoding an expected protein with a molecular weight of 52kDa. Based on the sequence similarity, this putative protein was designated as the B. gibsoni RAP-1 (BgRAP-1). The BgRAP-1 gene was expressed in the Escherichia coli BL21 strain, and the recombinant BgRAP-1 was used as the antigen in the enzyme-linked immunosorbent assay (ELISA). The results can differentiate between the B. gibsoni-infected dog sera and the Babesia canis infected dog sera or...
Palavras-chave: Babesia gibsoni; Rhoptry-associated protein; Enzyme-linked immunosorbent assay; Diagnosis.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/811

Comparative Evaluation of Mehods for the Diagnosis of Bovine Tritrichomonas foetus Infection
Provedor de dados: 19
Autores: Felleisen, Richard S. J.; Schimid-Lambelet, Natacha; Walubengo, J.
Palavras-chave: Diagnosis; In vitro culture; Polymerase chain reaction (PCR).
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/266

Utility of Serum Thymidine Kinase Activity Measurements for Cases of Bovine Leukosis with Difficult Clinical Diagnoses
Provedor de dados: 19
Autores: TAWFEEQ, Mohammad Monir; MIURA, Saori; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 猪熊, 壽.
This study evaluated the clinical usefulness of serum thymidine kinase (TK) activity for diagnosing bovine leukosis cases for which clinical diagnosis was difficult (‘BL with difficult diagnosis’). Median TK activity values in 24 ‘BL with difficult diagnosis’ and 36 cattle for which BL was clinically confirmed by cytology findings of enlarged superficial lymph nodes (‘clinically confirmed BL’) were 36.8 and 39.4 U/l, respectively (no significant difference). The percentage with positive TK activity (&gt;5.4 U/l) was also similar in both groups (83.3% for ‘BL with difficult diagnosis’ and 97.2% for ‘clinically confirmed BL’). TK activity was significantly higher in cows with ‘BL with difficult diagnosis’ compared to those with other tumors (N=13) and those...
Palavras-chave: Atypical bovine leukosis; Diagnosis; Thymidine kinase.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3869

Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes
Provedor de dados: 19
Autores: Thekisoe, Oriel M.M.; Rambritch, Natasha E.; Nakao, Ryo; Bazie, Raoul S.; Mbati, Peter; Namangala, Boniface; Malele, Imna; Skilton, Robert A.; Jongejan, Frans; Sugimoto, Chihiro; Kawazu, Shin-Ichiro; Inoue, Noboru.
We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South...
Palavras-chave: Theileria parva; LAMP; Buffalo; Cattle; Diagnosis.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2672

Diagnostic Methods for the Detection of Cryptosporidium parvum Oocysts in Faeces
Provedor de dados: 19
Autores: Webster, K. A.; Green, J. A.; Dawson, C.; Giles, M.; Catchpole, J.
Palavras-chave: Cryptosporidium parvum; Diagnosis; Immunofluorescence; ELISA; Coproscopical.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/253

Diagnosis of Trypanosoma evansi by the polymerase chain reaction (PCR)
Provedor de dados: 19
Autores: Donelson, J. E.; Artama, W. T.
Palavras-chave: Trypanosoma evansi; Diagnosis; Polymerase chain reaction.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/300

DIAGNÓSTICO DA AMBIÊNCIA ORGANIZACIONAL DA COMUNIDADE DE PRODUTORES DE PIMENTA LONGA (PIPER HISPIDINERVUM) UNIÃO DA LINHA SEIS DO PROJETO DE COLONIZAÇÃO HUMAITÁ EM PORTO ACRE – AC
Provedor de dados: 31
Autores: Teixeira,   Solange Maria Chalub Bandeira; Carvalho,   Lucas Araujo.
Este trabalho apresenta o resultado parcial de uma pesquisa maior sobre a avaliação ambiental e sócio-econômica da produção de pimenta longa (Piper hispidinervum) de uma comunidade de produtores de um projeto de colonização do INCRA. Trata-se da sistematização do que foi levantado em campo, através de pesquisa realizada com 10 (dez) produtores rurais, pertencentes a uma associação de produtores do Projeto de Colonização Humaitá (INCRA), no município de Porto Acre (Ac). O diagnóstico contemplou o levantamento de forças propulsoras e restritivas inerentes à ambiência de cada propriedade rural, elencadas numa matriz. Os resultados mostraram a necessidade de rever as políticas públicas e a gestão dos negócios rurais, de forma a dar subsídios e mais respaldo às...
Tipo: Conference Paper or Presentation
Palavras-chave: Avaliação ambiental e sócio-econômica; Pimenta longa; Comunidade de produtores rurais; Diagnóstico; Desenvolvimento sustentável; Políticas públicas; Environmental and socio-economical evaluation; Long pepper; Rural producers community; Diagnosis; Supporting development; Public politics; Crop Production/Industries.
Ano: 2008
URL: http://purl.umn.edu/109231

DIAGNÓSTICO DE LA CADENA OVINA EN LA PROVINCIA DE CÓRDOBA, ARGENTINA
Provedor de dados: 31
Autores: Aguero,   Daniel; Freire,   Viviana; Peralta,   Marcela; Vigliocco,   Mauricio; Sandoval,   Gabriela.
The continuous process toward agriculture is the main reason of the substitution and even disappearance of the ovine cattle of the province. The internal market is barely developed and strongly seasonal. The objective of this work is to characterize the chain of ovine meat of the province of Cordoba. The used data come from primary information: surveys and interviews to described informants, and of secondary information. Even though, the ovine production net has a complementary character of other cattle activities and agricultural, the present and potential demand of the lamb meat follows in increase. This study also has detected important restrictions for the integral development of the chain, the most notable aim to the tax burden, the sanitary problems...
Tipo: Journal Article
Palavras-chave: Diagnosis; Ovine meat chain; Cordoba-Argentina; FODA.; Agribusiness.
Ano: 2010
URL: http://purl.umn.edu/56557

Identification of turkey astrovirus and turkey coronavirus in an outbreak of Poult Enteritis and Mortality Syndrome
Provedor de dados: 89
Autores: Villarreal,LYB; Assayag,MS; Brandão,PE; Chacón,JLV; Bunger,AND; Astolfi-Ferreira,CS; Gomes,CR; Jones,RC; Ferreira,AJP.
This article reports a survey on turkey astrovirus (TAstV) and turkey coronavirus (TCoV) infections with RT-PCR in 17 turkey flocks affected by acute enteritis and two apparently normal turkey flocks located in the Southeastern region of Brazil by PCR (TAstV and TCoV). Seven out of the 17 affected flocks were positive for TAstV and 14 for TCoV, with seven co-infections. In one of the two apparently normal flocks, a TAstV-TCoV co-infection was found. Although a definitive association of these agents and the signs can not be made, the implications of these findings are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astrovirus; Coronavirus; Turkey; Enteritis; Diagnosis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000200010

Diagnosis of infectious bronchitis: an overview of concepts and tools
Provedor de dados: 89
Autores: Villarreal,LYB.
Infectious bronchitis (IB) casues multi-systemic infection in chickens with signs similar caused by other poultry pathogens and thus a high diagnostic accuracy can only be achieved by s series of laboratory assays. This article reviews in a brief way the traditional virus assays such as embryo innoculation, tracheal rings and virus neutralization assays for the direct detection of Avian infectious bronchitis virus (IBV) and methods based on gene molecular biology and some assays for the detection of anti-IBV antibodies, including ELISA. A critical view on each technique is also provived by the author.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Diagnosis; IBV; Typing.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200006

Enteric viruses in turkey flocks: a historic review
Provedor de dados: 89
Autores: Alavarez,JM; Ferreira,CSA; Ferreira,AJP.
In this review, diagnostic techniques and viral agents involved in enteric diseases affecting turkeys are described. Data from field observations and laboratory researches have been reported in turkey flocks for over 70 years, and several viruses have been identified. After a period of 30 years of inoculation experiments and neutralization studies, adequate visualization of the viruses was achieved using electronic microscopy. During the following years, several studies were then conducted to isolate and classify those viruses using cell-culture, embryo-propagation, serological tests, genome electropherotyping by polyacrylamide gel electrophoresis of double-stranded RNA viruses, and recently, nucleic acid studies. Thus, since the 1990s, the nucleic-acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Turkey; Enteritis; Viruses; Poults; Diagnosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300001

Litter characteristics and pododermatitis incidence in broilers fed a sorghum-based diet
Provedor de dados: 89
Autores: Carvalho,CMC; Litz,FH; Fernandes,EA; Silveira,MM; Martins,JM da S; Fonseca,LA; Zanardo,JA.
The aim of this study was to evaluate the incidence of footpad dermatitis and quality of broilers litter fed with sorghum grain and diets based in corn. It was used 544 male and female chicks, distributed in a completely randomized design with two treatments and eight replications. The chicken feed was formulated and produced from corn, soybean meal and sorghum grain. In the formulation were kept constant levels of energy and protein in accordance with the following treatments: A. Control (diet based on corn and soybean meal); B. Grain sorghum (Whole Sorghum + soybean meal). At 35 and 42 days were evaluated mineral matter (A %), calcium (Ca%), phosphorus (P%), pH and dry matter (DM %) of the poultry litter. To evaluate the footpad dermatitis were evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Turkey; Enteritis; Viruses; Poults; Diagnosis; Calcium; Environmental impact; Phosphorus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300010

Assessment of Broiler Chicken Welfare in Southern Brazil
Provedor de dados: 89
Autores: Federici,JF; Vanderhasselt,R; Sans,ECO; Tuyttens,FAM; Souza,APO; Molento,CFM.
Abstract Scientific literature on broiler chicken welfare in Brazilian industrial systems is scarce. This study aimed at assessing broiler chicken welfare on industrial farms in the State of Rio Grande do Sul, Southern Brazil, using the Welfare Quality(r) assessment protocol for poultry, to provide directly applicable scientific information. Results are presented as criteria scores ranging from 0 to 100, with higher scores indicating better welfare; and percentages of prevalence. The scores classified as excellent (above 80) were absence of prolonged thirst, absence of prolonged hunger, litter quality, breast blister and touch test. Enhanced scores (between 55 and 80) were comfort around resting, plumage cleanliness and dust sheet test. Acceptable scores...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal welfare; Assessment; Diagnosis; Poultry welfare; Welfare Quality(r).
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100133

Susceptibility of mammalian cell line for isolation of IBDV from clinical samples
Provedor de dados: 89
Autores: Silva,SEL; Ferreira,HL; Carvalho,BA; Cardoso,TC.
Susceptibility of chicken embryo related cells (CER) to non-classical IBDV was compared with Vero cells for the same purpose. It was used 20 bursal samples collected from clinical cases of IBDV, positive by histopathological evaluation. After three times infection of CER and Vero cells monolayers, at 6, 12, 18, 24, 48, 60 and 72h post-infection (PI), extra-cellular and intracelluar IBDV was measured. The extra-cellular amount of IBDV particles was quantified by DAS-ELISA from 6h PI, and increased for both cells studied until 72h PI. The IBDV re-isolation performed by infecting chicken embryos showed positive results at all PI studied. The intracellular IBDV RNA was shown by RT-PCR, where all tested samples were positive. However, the cytopathic effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avibirnavirus; CER cells; Diagnosis; Double antibody sandwich ELISA; RT/PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100009

Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction
Provedor de dados: 89
Autores: Meireles,MV; Roberto,LO; Riera,RF.
There are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Eimeria mitis; Eimeria praecox; Epidemiology; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400010

Avian mycoplasmosis update
Provedor de dados: 89
Autores: Nascimento,ER; Pereira,VLA; Nascimento,MGF; Barreto,ML.
Avian mycoplasmas occur in a variety of bird species. The most important mycoplasmas for chickens and turkeys are Mycoplasma gallisepticum (MG), M. synoviae (MS), and M. meleagridis. Besides, M. iowe (MI) is an emerging pathogen in turkeys, but of little concern for chickens. Mycoplasmas are bacteria that lack cell wall and belong to the class Mollicutes. Although they have been considered extracellular agents, scientists admit nowadays that some of them are obligatory intracellular microorganisms, whereas all other mycoplasmas are considered facultative intracellular organisms. Their pathogenic mechanism for disease include adherence to host target cells, mediation of apoptosis, innocent bystander damage to host cell due to intimate membrane contact,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Airsacculitis; Diagnosis; Mycoplasma; Mollicutes; Poultry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100001

Evolution and perspective of the temperate fruit crops in São Paulo state, Brazil
Provedor de dados: 94
Autores: Tecchio,Marco Antonio; Bettiol Neto,José Emilio; Barbosa,Wilson; Tucci,Maria Luiza Sant'ana.
This work was carried out to show the current situation of the temperate fruit crops in São Paulo state, Brazil, with an emphasis on grapes, peaches, apples, plums, nectarines and pears crops. Current economic data of crops, major growing regions, main cultivars produced, as well as the new technologies generated by research are presented. Regarding the grape crop, a decrease in the national production as well as in the major growing states was observed. The main grapes growing centers in São Paulo state are presented, highlighting its peculiarities regarding cultivars, cultural crop management and current researches. A trend has been observed toward increasing Niagara Rosada grape growing area rather than the fine table grape cultivars. It was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temperate fruit crops; Economics; Diagnosis; Cultural management.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000500018

Stephanofilariasis and nodular ulcerative dermatitis in dog: case report
Provedor de dados: 195
Autores: Novaes, Antonio Pereira de.
Objective: To report on the occurrence of stephanofilariasis in a dog, causing nodular ulcerative dermatitis in the abdominal area. Description: Dog patient, mixed breed, approximately 10 years old, presenting with nodular ulcerative dermatitis in the abdominal area. Suspected etiology was Stephanofilaria spp. AfieI' surgical excision of the lesion, anim print was performed, followed by fixation with ethanol and staining with 1:20 Giensa and saturated Congo resolution. The parasites (larval and adult forms) were identified under optical microscope (200 x). Results: The method employed allowed for visualization of the parasite, thus confirming the occurrence of stephanofilariasis in dogs. Conclusions: [t was demonstrated that Stephanofilaria spp can also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Filariasis; Diagnosis; Dermatitis; Skin ulcer; Drug therapy; Lvermectin; Dogs Filariosis; Diagnóstico; Dermatitis; Úlcera cutánea; Quimioterapia; Lvermectina; Perros filariose; Diagnóstico; Dermatite; Úlcera cutânea; Quimioterapia; Ivermectina; Cães.
Ano: 2005
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3129

Antebrachiocarpal joint synovial sarcoma in a dog
Provedor de dados: 195
Autores: Fonseca Pinto, Ana Carolina Brandão de Campos; Tatarunas, Angélica Cecilia; Chalita, Maria Carolina Catai; Michalany, Nilceo Schwery; Matera, Julia Maria.
Objective: To describe the clinical signs and radiographic, cytological and histopathological findings in a dog presented with antebrachiocarpal joint synovial sarcoma. The literature search was performed in CAB and MEDLINE databases, for the retrospective period of-t8 years. Description: A 9 year-old mongrel intact male dog was presented t0 the Small Animal Surgery Service of the Veterinary Hospital of the School of Veterinary Medicine and Animal Science of the University of São Paulo, São Paulo, SP, Brazil, for evaluation of a subcutaneous soft issue mass located cranial to the right antebrachiocarpal joint. Radiographic and cytologicalfindings suggested a malignant neoplasm, which was confirmed by the histopathological analysis to be a synovial sarcoma....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neoplasms; Sarcoma; Synovial; Diagnosis; Arthrography; Joints; Surgery; Amputation; Dogs Neoplasmas; Sarcoma sinovial; Diagnóstico; Artrografia; Articulaciones; Cirurgía; Amputación; Perros neoplasias; Sarcoma sinovial; Diagnóstico; Artrografia; Articulações; Cirurgia; Amputação; Cães.
Ano: 2004
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3235

Severe phlegmonous cystitis: case report
Provedor de dados: 195
Autores: Nakamuta, Bruna Higa; Kolber, Milton; Nagasse, Nathalie.
A case of severe phlegmonous cystitis or purulent cystitis due to bacterial infection is reported in a 9-year-old female dog of the Dachshund breed, presenting dysuria about a week ago. Biochemical examinations were performed, besides calculation analysis and abdominal ultrasound examination. The biochemistry exams did not present alterations. Calculation analysis showed calcium carbonate/urate. The diagnosis was confirmed by ultrasonography in which a heterogeneous, hypoechogenic and hyperechogenic aspect was seen in the caudal region of the bladder. The animal underwent surgery, in which a mass adhered to the bladder was removed and sent for histological examination, resulting in loss of epithelial lining and severe leukocyte infiltration. This report...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystitis; Diagnosis; Dog; Infection; Severe; Ultrasonography; Cão; Cistite; Diagnóstico; Infecção; Severa; Ultrassonografia.
Ano: 2019
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/37916

Redescripción de la obrera de Myrmelachista gallicola (Hymenoptera: Formicidae) y primera cita para la provincia de La Pampa (Argentina)
Provedor de dados: 99
Autores: Quirán,Estela M.; Martínez,Juan J.
Se redescribe la obrera de Myrmelachista gallicola Mayr y se cita por primera vez para la provincia de La Pampa (Argentina). El material estudiado está compuesto por obreras y fue recolectado sobre Prosopis caldenia Burkart (Fabaceae), dentro de las agallas de Tetradiplosis sp. (Diptera: Cecidomyiidae), en la periferia de la ciudad de Santa Rosa, provincia de La Pampa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrmelachista gallicola; Diagnosis; Distribución; Biología; Prosopis caldenia; Cecidomyiidae.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802006000200014

First record of the genus Kybos (Auchenorrhyncha: Cicadellidae: Typhlocybinae) for Argentina
Provedor de dados: 99
Autores: Catalano,María Inés; Paradell,Susana L.; Remes Lenicov,Ana M. de.
The genus Kybos Fieber and the species K populi (Edwards) are recorded for the first time for the Neotropical region from specimens collected in Mendoza province, Argentina. This contribution adds taxonomic and bioecologic knowledge about this genus and its species.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: First record; Diagnosis; Distribution; Host plant.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802012000100020

Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area
Provedor de dados: 63
Autores: Moura,Cátia Jacira Martins de; Fajardo,Thor Vinícius Martins; Eiras,Marcelo; Silva,Fábio Nascimento da; Nickel,Osmar.
ABSTRACT: The aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by real-time RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis; Diagnosis; Variability; Incidence; Leafroll.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000100043

Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
Provedor de dados: 102
Autores: Ríos,Melina Ocampo; Fernández,Paula; Carmona,Marcelo.
Leaf scald of barley caused by Rhynchosporium secalis is an important disease in Argentina. The fungus is a necrotrophic pathogen which survives in stubble, seeds and weeds. Isolation of R. secalis from seeds on artificial media usually has not been successful due to the slow growth rate of the pathogen and strong inhibition by contaminants. The objective in this work was to detect R. secalis in different genotypes of barley seeds in Argentina using the polymerase chain reaction (PCR)-based diagnostic assay. Four barley genotypes were tested in 2004: Quilmes Ayelén, Quilmes Alfa, Barke and Maltería Pampa 1004. The previously described RS8 and RS9 primers were used for the detection of R. secalis in barley seeds. A 264-bp single band was obtained for each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hordeum vulgare; Diagnosis; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500007

RT-PCR and Dot Blot hybridization methods for a universal detection of tospoviruses
Provedor de dados: 102
Autores: EIRAS,MARCELO; RESENDE,RENATO O.; MISSIAGGIA,ALEXANDRE A.; ÁVILA,ANTÔNIO C. DE.
Transcriptase reverse - polymerase chain reaction (RT-PCR) and dot blot hybridization with digoxigenin-labeled probes were applied for the universal detection of Tospovirus species. The virus species tested were Tomato spotted wilt virus, Tomato chlorotic spot virus, Groundnut ringspot virus, Chrysanthemum stem necrosis virus, Impatiens necrotic spot virus, Zucchini lethal chlorosis virus, Iris yellow spot virus. Primers for PCR amplification were designed to match conserved regions of the tospovirus genome. RT-PCR using distinct primer combinations was unable to simultaneously amplify all tospovirus species and consistently failed to detect ZLCV and IYSV in total RNA extracts. However, all tospovirus species were detected by RT-PCR when viral RNA was used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Plant viruses; Bunyaviridae.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000200009

Optical devices evaluation for diagnosis of Plasmopara viticola
Provedor de dados: 111
Autores: Aldo Calcante; Department of Agricultural Engineering, Università degli Studi di Milano, via Celoria 2- 20133 Milan; Aira Mena; Department of Agricultural Engineering, Università degli Studi di Milano, via Celoria 2- 20133 Milan; Fabrizio Mazzetto; Faculty of Science and Technology, Free University of Bozen, piazza Università, 5 – 39100 Bozen.
Remote sensing (RS) is the most widely adopted technique for crop monitoring in precision viticulture (PV).  Recent research looks at the development of proximal sensing technologies alternative to RS.  The present work considers the possible use of proximal sensing optical devices for diagnosis in vineyard; in particular, we evaluated the GreenSeeker RT100 and the Crop Circle (two commercial optical sensors) in detecting different levels of grapevine downy mildew symptoms.  The analysis was conducted on vine leaves that had been picked from plants of cv.  Cabernet Franc infected by Plasmopara viticola.  Leaves were divided into homogeneous infection classes and then analyzed through the optical devices and a portable Vis/NIR (visible/near infrared)...
Palavras-chave: Agricultural Engineering Precision viticulture; Diagnosis; NDVI; Proximal sensing; Optical devices.
Ano: 2011
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/1827

Sporothrix schenckii and sporotrichosis
Provedor de dados: 42
Autores: Lopes-Bezerra,Leila M.; Schubach,Armando; Costa,Rosane O.
For a long time sporotrichosis has been regarded to have a low incidence in Brazil; however, recent studies demonstrate that not only the number of reported cases but also the incidence of more severe or atypical clinical forms of the disease are increasing. Recent data indicate that these more severe forms occur in about 10% of patients with confirmed diagnosis. The less frequent forms, mainly osteoarticular sporotrichosis, might be associated both with patient immunodepression and zoonotic transmission of the disease. The extracutaneous form and the atypical forms are a challenge to a newly developed serological test, introduced as an auxiliary tool for the diagnosis of unusual clinical forms of sporotrichosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sporotrichosis; Diagnosis; Epidemiology; Drugs; Cell wall; Antigens.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000200009

A scientific survey on 1550 cases of oral lesions diagnosed in a Brazilian referral center
Provedor de dados: 42
Autores: VASCONCELOS,ARTUR C.; ABURAD,CARLOS; LIMA,IGOR F.P.; SANTOS,SHEILA M.M.; FREITAS FILHO,SILAS ANTONIO J. DE; FRANCO,ADEMIR; RODE,SIGMAR M.; PARANHOS,LUIZ R.
ABSTRACT The present study aimed to perform an epidemiological survey on the prevalence rate of oral lesions diagnosed in a referral center in Brazil and to establish association between the lesions and the age and gender of the affected patients. This analytical observational study analyzed 3521 medical records of anatomopathological exams of the Pathological Anatomy and Cytopathology Laboratory of the Cancer Hospital of the state of Mato Grosso (Brazil), from December 2011 to September 2015. A total of 1550 relevant medical records was detected. Patients aging between 41 and 60 consisted of 42.84% of the sample. Males were more affected than females (60.13%). Inflammatory processess consisted of the most prevalent diagnosis (43.29%), followed by squamous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Oral medicine; Pathology; Stomatology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401691

Perception, knowledge and attitudes of Brazilian orthodontists on the treatment of Class II malocclusions
Provedor de dados: 42
Autores: KAIEDA,ARMANDO K.; LIMA,IGOR F.P.; SCANAVINI,MARCO ANTÔNIO; COQUEIRO,RAILDO S.; PITHON,MATHEUS M.; RODE,SIGMAR M.; PARANHOS,LUIZ RENATO.
ABSTRACT The present study aimed to assess the perception and knowledge of Brazilian orthodontists on the ideal moment to treat Class II malocclusions. Questionnaires with open, semi-open and close questions were sent by e-mail to 1653 Brazilian orthodontists. These orthodontists were registered in the Brazilian Association of Orthodontics and Dentofacial Orthopedics (ABOR). One-hundred and three (9.86%) Orthodontists replied to the questionnaires. Most of them were males (60.5%) with specialization as the highest level of professional qualification (59.9%). Most of the orthodontists (51%) reported preference for treating Class II malocclusions in late mixed dentition, followed by the early mixed dentition (29%). The age range between 10 and 12 years old...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orthodontic appliances; Attitudes; Diagnosis; Class II malocclusion.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602875

Subclinical Diabetes
Provedor de dados: 42
Autores: LIMA,LUÍS M.T.R.
ABSTRACT Type 2 diabetes mellitus (T2DM) is increasing in prevalence worldwide, and those non-diagnosed or misdiagnosed comprise a significant group compared to those diagnosed. Accumulated scientific evidence indicate that the current diagnostic markers (fasting glycemia, 2h glycemia after an oral glucose load and HbA1c) are indeed late diagnostic criteria when considering the incidence of diabetes-related complications and comorbidities, which are also at high risk in some groups among normoglycemic individuals. Additionally, the earlier identification of future risk of diabetes is desirable since it would allow better adherence to preventive actions such as lifestyle intervention, ultimately avoiding complications and minimizing the economic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Diagnosis; Insulin; Subclinical diabetes; Metabolic syndrome.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200591

Modification of the Alere GIARDIA Ag TEST immunochromatography KIT methodology for its use in frozen fecal sediment of dogs and cats
Provedor de dados: 42
Autores: COSTA,VIVIANE A.N.; BRENER,BEATRIZ; FONSECA,ANA BEATRIZ M.; SUDRÉ,ADRIANA P.
ABSTRACT Giardia duodenalis is a worldwide intestinal parasite and is one of the most frequent protozoa species infecting dogs and cats. This study aimed to modify the methodology of Alere GIARDIA Ag TEST KIT for its use in frozen fecal sediments with different storage times in a freezer (-20°C), thus expanding the range of use of this methodology. One hundred fecal sediments from dogs (n=50) and cats (n=50) previously examined by optical microscopy for Giardia cysts were selected for this study. The agreement between the modified immunochromatography and microscopy results was calculated by Kappa coefficient. To evaluate the performance of the modified immunochromatography assay on samples with different storage time, the fecal sediments were divided into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cats; Diagnosis; Dogs; Giardia duodenalis; Immunochromatography.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100479

Real-time PCR for detection of Brucella ovis and Histophilus somni in ovine urine and semen
Provedor de dados: 45
Autores: Moustacas,V.S.; Silva,T.M.A.; Costa,É.A.; Costa,L.F.; Paixão,T.A.; Santos,R.L.
RESUMO A epididimite infecciosa ovina é uma das principais enfermidades reprodutivas de carneiros. O presente estudo teve por objetivo desenvolver protocolos de PCR em tempo real para B. ovis e H. somni e avaliar sua aplicabilidade em amostras de sêmen e urina de carneiros. Delinearam-se primers e sondas espécie-específicos para cada agente. As sondas foram delineadas com o sistema TaqMan incorporando um marcador FAM para B. ovis e Cy5 para H. somni na extremidade 5' e um quencher na extremidade 3'. A PCR em tempo real para B. ovis e H. somni foi altamente sensível, uma vez que a amplificação de DNA ocorreu com até 0,2ng de DNA/reação. A especificidade dos iniciadores e sondas foi avaliada com amostras de DNA de outros agentes causadores de epididimite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious epididymitis; Ovine; Diagnosis; PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601751

Meningoencephalitis in a horse associated with equine herpesvirus 1
Provedor de dados: 45
Autores: Costa,E.A.; Lima,G.B.L.; Castro,R.T.; Furtini,R.; Portilho,R.V.; Resende,M.
Um garanhão da raça Brasileiro de Hipismo, de 12 anos de idade, apresentou um quadro neurológico complexo, que se assemelhava, em vários aspectos, aos sinais neurológicos causados pelo herpesvírus eqüino 1 (EHV-1), variando desde uma suave ataxia à completa paralisia dos posteriores. O animal morreu com menos de 12 horas após o início dos sinais nervosos. O diagnóstico de EHV-1 foi feito pela detecção do DNA viral no sistema nervoso central por meio da reação em cadeia da polimerase (PCR) e posterior sequenciamento.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine herpesvirus 1; Neurological signs; Clinical case; Diagnosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600044

Aspectos clínicos e radiográficos de caprino com megaesôfago
Provedor de dados: 45
Autores: Silva Júnior,L.C.; Arruda,L.C.P.; Silva,D.G.B.; Soares,F.A.P.; Borba Neto,A.V.; Leite,J.E.B.; Soares,P.C.
A seven month old female goat showed neck swelling, apathy, appetite and weight loss, restlessness, increased salivation, cough, and regurgitation episodes which occurred post feeding. The animal was evaluated through both clinical and radiographic examinations. Plain radiography was performed and contrasted X-ray was done using barium sulfate. For radiological examination, the lateral projection was used for analysis. The results showed a radiopaque content in the esophageal lumen dorsally positioned to heart with density and appearance similar to the rumen. A dilation of the esophagus was reported and a diagnosis of megaesophagus was made. In conclusion, contrast esophagography must be included in the diagnosis of megaesophagus in goats.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Goat; Esophagus; Radiography; Diagnosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300033

Clinical diagnosis and alternative surgical treatment of tetralogy of Fallot in a dog. A case report
Provedor de dados: 45
Autores: Larsson,M.H.M.A; Pereira,L.; Jatene,F.B.; Freitas,R.F.; Barbusci,L.O.D.; Oliveira,S.M. de; Abduch,M.C.D.
Tetralogy of Fallot was diagnosed in a 30 month-old Cocker Spaniel female, through physical, radiographic, electrocardiographic, and echocardiographic exams. The animal was submitted to a palliative surgical correction, the Blalock-Taussig technique, and 46 months after the surgery, no signs of cardiopulmonary decompensation were observed.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Dog; Tetralogy of Fallot; Diagnosis; Surgical correction.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000500003

Winter dysentery in cows associated with Bovine Coronavirus (BCoV)
Provedor de dados: 45
Autores: Brandão,P.E.; Gregori,F.; Sforsin,A.J.; Villarreal,L.Y.B.; Jerez,J.A.
Descreve-se a pesquisa de BCoV e rotavírus em 13 mostras fecais de vacas de surtos de disenteria utilizando uma nested PCR dirigida ao gene RdRp e PAGE, respectivamente. Todas as amostras fecais foram positivas para BCoV e nenhuma delas apresentou-se positiva para rotavírus em PAGE. O encontro de coronavírus bovino em amostras fecais de vacas com disenteria sugere que este vírus possa ser o agente primário envolvido na etiologia dos casos aqui relatados.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine; Winter dysentery; Coronavirus; PCR; Diagnosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400038

Evaluation of western blotting for the diagnosis of enzootic bovine leukemia
Provedor de dados: 45
Autores: Gonzalez,E.T.; Oliva,G.A.; Norimine,J.; Cid de la Paz,V.; Echeverría,M.G.
A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally BLV infected cows were used. An agar gel immunodiffusion test (AGID) was used for comparing with the results obtained by WB. The AGID positive sera showed a different degree of reactivity by WB test against the two most important viral antigens (gp51 and p24), or against one of them. Other proteins (gp30, p15, p12 and p10) were not detected with any AGID positive sera, being observed occasionally three bands corresponding to the p24 protein. Using sera obtained by BLV experimental inoculation, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine leukosis; Western blotting; Diagnosis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400002

Reverse transcription-polymerase chain reaction assay for rabies virus detection
Provedor de dados: 45
Autores: Dantas Junior,J.V.; Kimura,L.M.S.; Ferreira,M.S.R.; Fialho,A.M.; Almeida,M.M.S.; Grégio,C.R.V.; Romijn,P.C.; Leite,J.P.G.
Este estudo teve por objetivo implantar um protocolo de amplificação genômica, precedida de transcrição reversa (RT-PCR) para o gene da nucleoproteína do vírus da raiva, para a utilização dessa metodologia em laboratórios onde são realizadas investigações para a detecção do vírus rábico. Foram utilizadas 50 amostras de tecido encefálico de animais (44 bovinos, 5 eqüinos e 1 quiróptero) oriundos do Estado do Rio de Janeiro, positivos por imunofluorescência direta e/ou prova biológica para o vírus rábico. A extração do RNA foi feita a partir da suspensão a 10% em PBS pH7,2 do tecido encefálico utilizando-se a metodologia de TRIzolTM (Life Technologies) e o protocolo de RT-PCR descrito por Heaton et al. (1997), incluindo algumas modificações. Dentre as 50...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rabies virus; Reverse transcription-polymerase chain reaction; Diagnosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300017

Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
Provedor de dados: 45
Autores: Kleiman,F.; Pietrokovsky,S.; Gil,S.; Wisnivesky-Colli,C.
The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P&lt;0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Fasciola hepatica; Coprological method; Diagnosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000200007

Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis
Provedor de dados: 45
Autores: Campos,M.P.; Madeira,M.F.; Silva,D.A.; Solcà,M.S.; Espíndola,O.M.; Mendes Júnior,A.A.V.; Figueiredo,F.B.
ABSTRACT The purpose of the present work was to evaluate the accuracy of quantitative polymerase chain reaction (qPCR) performed on samples of fresh frozen tissue (FT) and formalin-fixed, paraffin-embedded (FFPE) healthy skin. This is a validation study conducted with samples from 46 dogs from an endemic area in Brazil. After sample collection, DNA extractions were conducted using commercial kits and qPCR was oriented to kinetoplast DNA (kDNA) targets of the Leishmania infantum species. The results obtained for the FFPE samples showed 63.6% sensitivity and 77.1% specificity, whereas those obtained for the FT samples showed 100% and 48.6%, respectively. Poor agreement was observed for the results of the qPCR technique with FT and FFPE samples. Our results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; QPCR; Canine visceral leishmaniasis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601443

Bronchoalveolar lavage (BAL) in tapirs (Tapirus terrestris) with Mycobacterium tuberculosis
Provedor de dados: 45
Autores: Biava,J.S.; Gonçalves,R.C.; Javorouski,M.L.; Bonat,M.; Lacerda,O.; Murakami,P.S.; Vilani,R.G.D.C.; Biondo,A.W.
This paper describes the in vivo Bronchoalveolar lavage (BAL) technique by endoscopy in tapirs (Tapirus terrestris) with clinical signs of tuberculosis. The technique was performed in two tapirs, male and female, from Curitiba Zoo, Paraná, Brazil. A flexible endoscope and a polyethylene catheter were used after the chemical restraint of the animals. For BAL technique, 60mL of saline 0.9% were infused with a polyethylene catheter, introduced by the endoscope's working channel, and 15mL of BAL were recovered, analyzed and submitted to cytocentrifugation. Slides were stained by Papanicolaou, periodic acid-Schiff (PAS) and Ziehl-Neelsen methods contained high quantity of inflammatory cells on light microscopy (macrophages 27.5%, lymphocytes 0.5%, neutrophis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tapirs; Diagnosis; Endoscopy; Tuberculosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601643

Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot
Provedor de dados: 45
Autores: Silva,L.F.; Pinto,P.S.A.; Ducas,C.T.S.; Santos,T.O.; Nieto,E.C.A.; Peixoto,R.P.M.G.
Given the limited knowledge about the diagnosis of bovine cysticercosis by immunoblot, the aim of this study was to assess the applicability of this test, identifying key peptides with diagnostic value. Immunoblot assays were performed using total larval antigen of Taenia crassiceps and 60 sera of positive bovines for cysticercosis (30 naturally and 30 experimentally infected with T. saginata eggs), 30 sera of negative bovines for cysticercosis and 30 sera of bovines with other diseases (fascioliasis, hydatidosis and tuberculosis). The peptides of greater diagnostic importance, in descending order of accuracy (%), were as follows: 6-8kDa (90.8%), 129-143kDa (74.2%), 99-105kDa (71.7%) and 14-19kDa (71.1%). Cross-reactions, due to fascioliasis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine cysticercosis; Diagnosis; Immunoblot; Taenia crassiceps; Peptides.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300891

Confirmation and Sequence analysis of N gene of PPRV in South Xinjiang, China
Provedor de dados: 45
Autores: Liu,YongHong; Yang,Bo; Li,XianQiang; Wang,XueFeng; Zhang,LuYao; Li,Fei; Zhao,Li.
ABSTRACT In China, Peste des petits ruminants (PPR) was officially first reported in 2007. From 2010 until the outbreak of 2013, PPRV infection was not reported. In November 2013, PPRV re-emerged in Xinjiang and rapidly spread to 22 P/A/M (provinces, autonomous regions and municipalities) of China. In the study, suspected PPRV-infected sheep in a breeding farm of South Xinjiang in 2014 were diagnosed and the characteristics of complete sequence of N protein gene of PPRV was analyzed. The sheep showed PPRV-infected signs, such as fever, orinasal secretions increase, dyspnea and diarrhea, with 60% of morbidity and 21.1% of fatality rate. The macroscopic lesions after autopsy and histopathological changes were observed under light microscope including...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PPR; Diagnosis; N gene; Sequence analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501105

Evaluation of different sites for blood collection for β-hydroxybutyrate assessment in dairy cows using hand-held meter
Provedor de dados: 45
Autores: Alvarenga,E.A.; Moreira,G.H.F.A.; Facury Filho,E.J.; Leme,F.O.P.; Moreira,T.F.; Carvalho,A.U.
RESUMO Cetose subclínica é uma grande preocupação em rebanhos leiteiros, e seu diagnóstico e prevenção podem ter um grande impacto na saúde animal. Este estudo comparou quatro locais diferentes para a coleta de amostras de sangue (jugular, veias mamárias e coccígeas e ponta da cauda) para medição de β-hidroxibutirato (BHBA), usando-se um medidor portátil automático. Foram utilizadas seis vacas Holandesas, e a coleta de sangue foi feita no segundo, quinto, 10º, 15º e 21º dias pós-parto. Os resultados do medidor portátil foram semelhantes aos resultados do laboratório e apresentaram uma correlação forte de 0,83. As concentrações séricas de BHBA nas amostras de sangue coletadas na ponta da cauda, na jugular e na coccígea foram semelhantes. No entanto, o...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dairy cows; Ketosis; Transition period; Diagnosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401425

Altered indirect hemagglutination method for easy serotyping of Haemophilus parasuis
Provedor de dados: 45
Autores: Lorenson,M.S.; Miani,M.; Guizzo,J.A.; Barasoul,B.; Martínez-Martínez,S.; Rodríguez-Ferri,E.F.; Gutiérrez-Martín,C.B.; Kreutz,L.C.; Frandoloso,R.
ABSTRACT Glässer's disease is an emergent bacterial disease that affects swine husbandries worldwide causing important economic losses. The aetiological agent, Haemophilus parasuis, is currently divided in fifteen serovars but an increasing number of non-typeable serovars have been reported. Indirect hemagglutination (IHA) is indicated as a serotyping method for H. parasuis. In the present study, we describe an additional step that aims to work around a possible obstacle in the original protocol that may compromise the outcome of this assay. We observed that the choice of anticoagulant for blood collection influences and/or impairs spontaneous adsorption of H. parasuis antigens on sheep red blood cells (SRBCs). However, regardless of the anticoagulant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haemophilus parasuis; Diagnosis; Tannic acid; Serotyping; Indirect hemagglutination.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000100015

Diagnosis of Brucella ovis infection by serology and PCR in urine samples from naturally infected rams in the state of Piauí
Provedor de dados: 45
Autores: Costa,E.A.; Sant'Ana,F.M; Carvalho,C.J.S.; Moustacas,V.S.; Silva,S.M.M.S.; Paixão,T.A.; Santos,R.L.
A brucelose ovina causada pela Brucella ovis é uma doença reprodutiva de carneiros caracterizada por epididimite, orquite, com consequente diminuição da fertilidade e prejuízos econômicos significativos. O presente trabalho teve por objetivo avaliar a aplicabilidade da técnica de PCR como um método de diagnóstico em campo, comparado-a com a técnica de IDGA. Foram coletadas amostras de urina e soro de 90 carneiros oriundos de 31 rebanhos localizados no Estado do Piauí. Quatro das 31 (12,9%) propriedades avaliadas apresentaram animais positivos. Dezoito (20%) amostras de urina foram positivas pela PCR, enquanto o método de IDGA identificou 16 (17,8%) carneiros soropositivos. Embora os métodos tenham apresentado concordância baixa na estatística Kappa...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella ovis; Sheep; Diagnosis; PCR; AGID.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300029

Morphological and molecular characterization of Curvularia lunata pathogenic to andropogon grass
Provedor de dados: 53
Autores: Santos,Patrícia Resplandes Rocha dos; Leão,Evelynne Urzêdo; Aguiar,Raimundo Wagner de Souza; Melo,Maruzanete Pereira de; Santos,Gil Rodrigues dos.
ABSTRACT The fungal genus Curvularia is associated with a number of diseases in plants, commonly producing foliar spots in forage grasses. The objective of this study was to characterize the morphological and molecular diversity of the isolates of Curvularia sp. associated with Andropogon seeds, and to assess both their capacity to transmit disease and the pathogenicity of this fungus to crop. Ten isolates of Curvularia sp. were sourced from Andropogon seeds from agricultural producing regions in the Brazilian states Tocantins and Pará. Morphological characterization was achieved by observing fungus colonies and conidia and molecular characterization by DNA extraction and amplification with sequence-specific primers. The disease transmission was evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Andropogon L.; Curvularia lunata; Diagnosis; Phylogeny; Pathogen.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000200326

Hematological and cerebrospinal fluid changes in cattle naturally and experimentally infected with the bovine herpesvirus 5
Provedor de dados: 52
Autores: Lisbôa,Júlio Augusto Naylor; Isernhagen,Allan Jürgen; Borges,Alexandre Secorun; Amorim,Rogério Martins; Balarin,Mara Regina Stipp; Lunardi,Michele; Alfieri,Amauri Alcindo.
Bovine herpesvirus 5 (BoHV-5) meningoencephalitis is one of the main causes of mortality by encephalopathy in Brazilian cattle herds. However, the neurological signs observed are common to several encephalopathies and do not contribute decisively to a diagnosis. In order to verify hematological and cerebrospinal fluid (CSF) changes, blood and CSF samples from naturally and experimentally infected bovines were evaluated. In natural cases (n=17), the samples were collected only once, and in experimental cases (n=7), the samples were sequentially obtained throughout disease progression. While routine methods were used to examine the samples, BoHV-5 infection was confirmed by a PCR assay. Blood analyses did not reveal any consistent hematological alterations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Meningoencephalitis; Herpesviruses; Complete blood count; Cerebrospinal fluid; Diagnosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700010

Radiopeptides from diagnosis to therapy
Provedor de dados: 52
Autores: Buscombe,John. R.
Radiopeptides are playing an increasing role in the diagnosis of disease. Initially based on the somatatostatin system, early research concentrated on identifying tumours of neuroendocrine origin. New techniques with artificial peptides are extending their role into other areas such as thrombus formation and heart disease. It has been shown that radiopeptides have excellent labelling new methods have looked at therapy starting with high activities of In-111 pentetreotide to the development of Y-90 based products and new and novel peptides and radio isotopes. Outside of PET there are the most exciting developments in nuclear medicine.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopeptides; Diagnosis; Therapy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500013

High molecular diversity of the fungus Guignardia citricarpa and Guignardia mangiferae and new primers for the diagnosis of the citrus black spot
Provedor de dados: 52
Autores: Stringari,Danyelle; Glienke,Chirlei; Christo,Daniel de; Maccheroni Jr.,Walter; Azevedo,João Lucio de.
RAPD markers were used to investigate the distribution of genetic variability among a group of Guignardia citricarpa, G. mangiferae, and Phyllosticta spinarum isolates obtained from several hosts in Brazil, Argentina, Mexico, Costa Rica, Thailand, Japan, United States and South Africa. Pathogenic isolates G. citricarpa Kiely (anamorph form P. citricarpa McAlp Van Der Aa) are the etiological agent of the Citrus Black Spot (CBS), a disease that affects several citric plants and causes substantial injuries to the appearance of their fruits, thus preventing their export. Several previous studies have demonstrated the existence of an endophytic species with high morphological similarity to the causal agent of CBS that could remain latent in the same hosts....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Guignardia; SCARS; Citrus Black Spot; Citrus; Diagnosis; RAPD.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500002

Serological survey for bovine herpesvirus 1 in cattle from different regions in the state of Bahia, Brazil
Provedor de dados: 62
Autores: CERQUEIRA,Robson Bahia; CARMINATI,Renato; SILVA,Josiane Martins; SOARES,Gubio Campos; MEYER,Roberto; SARDI,Silvia.
The presence of antibodies against BHV-1 was evaluated in young bovines from fifteen different counties distributed along North, South and Central regions of the state of Bahia. Bovines with age ranged from 1 to 4 years old, non-vaccinated against BHV-1 and without diagnostic of BHV-1 infection were chosen for blood collection. A total of 558 serum samples were collected equivalent to 10% of the population in each farm. The sera were submitted to ELISA (HerdCheck-IDDEX) and Microserum neutralization (MSN) tests. The MSN was performed in monolayers of MDBK cells in microplates using Los Angeles BHV-1 strain. The results of serum samples by ELISA showed that 56% (314/558) were positive and 44% (244/558) were negative. The sera assayed by MSN showed that 48%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine herpesvirus 1 (BHV-1); ELISA; Serumneutralization; Diagnosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600014

Evaluation of experimental Toxoplasma gondii (Nicolle and Manceaux, 1909) infection in pigs by bioassay in mice and polymerase chain reaction
Provedor de dados: 62
Autores: Yai,Lucia Eiko Oishi; Vianna,Manoella Compostrini Barreto; Soares,Rodrigo Martins; Cortez,Adriana; Freire,Roberta Lemos; Richtznhain,Leonardo José; Gennari,Solange Maria.
The aim of the present experiment was to standardize a nested polymerase chain reaction (nPCR) for the detection of Toxoplasma gondii in tissues of experimentally infected pigs and to compare the performance of nPCR with the standard isolation technique, the bioassay in mice. Comparison between the two methods was done testing eight 4 month-old pigs orally inoculated with 5 x 10(4) oocysts of Toxoplasma gondii (AS-28 strain) and three non-infected pigs at the same age, kept as control. All animals were euthanatized 47 days after infection and samples of brain, heart, tongue and retina were collected from each animal for analysis by nPCR and bioassay in mice. By using the bioassay, Toxoplasma gondii was detected in 4 infected pigs, being two in the retina,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Pig; NestedPCR; Bioassay; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000300010

Neurobrucellosis: clinical, diagnostic, therapeutic features and outcome. Unusual clinical presentations in an endemic region
Provedor de dados: 55
Autores: Ceran,Nurgul; Turkoglu,Recai; Erdem,Ilknur; Inan,Asuman; Engin,Derya; Tireli,Hulya; Goktas,Pasa.
Brucellosis is a zoonotic infection and has endemic characteristics. Neurobrucellosis is an uncommon complication of this infection. The aim of this study was to present unusual clinical manifestations and to discuss the management and outcome of a series of 18 neurobrucellosis cases. Initial clinical manifestations consist of pseudotumor cerebri in one case, white matter lesions and demyelinating syndrome in three cases, intracranial granuloma in one case, transverse myelitis in two cases, sagittal sinus thrombosis in one case, spinal arachnoiditis in one case, intracranial vasculitis in one case, in addition to meningitis in all cases. Eleven patients were male and seven were female. The most prevalent symptoms were headache (83%) and fever (44%). All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurobrucellosis; Serology; Diagnosis; Neuroimaging.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100010

Challenging Mycobacterium tuberculosi s dormancy mechanisms and their immunodiagnostic potential
Provedor de dados: 55
Autores: Chaves,Alexandre Silva; Rodrigues,Michele Fernandes; Mattos,Ana Márcia Menezes; Teixeira,Henrique Couto.
ABSTRACT Mycobacterium tuberculosis is the etiologic agent of tuberculosis, one of the world's greatest cause of morbidity and mortality due to infectious disease. Many evolutionary mechanisms have contributed to its high level of adaptation as a host pathogen. Prior to become dormant, a group of about 50 genes related to metabolic changes are transcribed by the DosR regulon, one of the most complex and important systems of host-pathogen interaction. This genetic mechanism allows the mycobacteria to persist during long time periods, establishing the so-called latent infection. Even in the presence of a competent immune response, the host cannot eliminate the pathogen, only managing to keep it surrounded by an unfavorable microenvironment for its growth....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Latency; DosR antigens; Diagnosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600636

Congenital toxoplasmosis in a reference center of Paraná, Southern Brazil
Provedor de dados: 55
Autores: Capobiango,Jaqueline Dario; Breganó,Regina Mitsuka; Navarro,Italmar Teodorico; Rezende Neto,Claudio Pereira; Casella,Antônio Marcelo Barbante; Mori,Fabiana Maria Ruiz Lopes; Pagliari,Sthefany; Inoue,Inácio Teruo; Reiche,Edna Maria Vissoci.
This study describes the characteristics of 31 children with congenital toxoplasmosis children admitted to the University Hospital of Londrina, Southern Brazil, from 2000 to 2010. In total, 23 (85.2%) of the mothers received prenatal care but only four (13.0%) were treated for toxoplasmosis. Birth weight was &lt;2500 g in 37.9% of the infants. During the first month of life, physical examination was normal in 34.5%, and for those with clinical signs and symptoms, the main manifestations were hepatomegaly and/or splenomegaly (62.1%), jaundice (13.8%), and microcephaly (6.9%). During ophthalmic examination, 74.2% of the children exhibited injuries, 58.1% chorioretinitis, 32.3% strabismus, 19.4% microphthalmia, and 16.2% vitreitis. Anti-Toxoplasma gondii IgM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Congenital toxoplasmosis; Chorioretinitis; Diagnosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400364

Seroprevalence of human cysticercosis in Jataí, Goiás state, Brazil
Provedor de dados: 55
Autores: Guarda,Kaliny Xavier da; Costa-Cruz,Julia Maria; Barcelos,Ivanildes Solange da Costa.
ABSTRACT The Taenia solium cysticercosis affects millions of people worldwide and is considered a public health problem, especially in developing countries. The diagnosis of neurocysticercosis is complex and involves the analysis of epidemiological, clinical, neuroimaging, and immunological host data. Neurocysticercosis is endemic in Brazil, and is related to the cause of death mainly in the Southeast, South, and Central-West regions. The objective of this study was to determine the seroprevalence of cysticercosis in inhabitants of the city of Jataí, Goiás, in the Central-West region of Brazil from April to August 2012. A total of 529 serum samples were analyzed by enzyme-linked immunosorbent assay for detecting IgG antibodies against T. solium larvae, and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cysticercosis; Taenia solium; Epidemiology; Diagnosis; Brazil.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200146

Predictive model for the diagnosis of tuberculous pleural effusion
Provedor de dados: 55
Autores: Neves,Denise Duprat; Dias,Ricardo Marques; Cunha,Antônio José Ledo A. da.
This study developed a predictive model to identify pleural tuberculosis. A consecutive cases study of patients investigating the cause of pleural effusion, in an area of high prevalence of tuberculosis (Rio de Janeiro, Brazil). Clinical and laboratory variables were compared among patients with tuberculosis (TB) and without tuberculosis (NTB), individually and using logistic regression. The performance was described as diagnostic accuracy, compared to a gold standard in a masked way. We have studied 104 TB patients, 41 with malignant, 29 transudates, 28 parapneumonic, 13 with miscellaneous diseases. After identification of individual discrimination power aided by clinical, radiological and laboratory variables, the following ones were included in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sensitivity and specificity; Tuberculosis; Pleural effusions; Predictive model; Diagnosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100019

Pseudotumoral form of primary progressive tuberculosis: a diagnosis to be considered
Provedor de dados: 55
Autores: Franco,Rosana; Santana,Maria Angélica; Coelho Filho,João Carlos; Pereira-Silva,Jorge L.
The diversity of clinical presentations of primary progressive tuberculosis (TB) and the difficulty in establishing the diagnosis of paucibacillary forms is the subject of painstaking research, as well as a cause of delay in therapy. We report the case of a 10-year-old black child who presented with chest pain and progressive widening of the upper mediastinum. Computerized tomography of the chest revealed multiple calcifications that were not identified with X-rays. Biopsy through mediastinoscopy was compatible with a diagnosis of tuberculosis. Despite exhaustive investigation that included direct examination, culture for mycobacteria and PCR (Polymerase Chain Reaction) of tissue samples, the etiologic agent was not revealed. Tuberculin conversion was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymph node tuberculosis; Pseudotumoral form; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200011

Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil
Provedor de dados: 55
Autores: Camilo,Lilian Muniz; Pereira-Chioccola,Vera Lucia; Gava,Ricardo; Meira-Strejevitch,Cristina da Silva; Vidal,Jose Ernesto; Mattos,Cinara Cássia Brandão de; Frederico,Fábio Batista; De Mattos,Luiz Carlos; Spegiorin,Lígia Cosentino Junqueira Franco; Murata,Fernando Henrique Antunes; Ferreira,Marina Neves; Barbosa,Deusenia Machado Ulisses; Gonçalves,Fausto da Silva; Dias,Cristiane Moraes; Catelan,Marcia Wakai; Siqueira,Rubens Camargo; Previato,Mariana; Barbosa,Amanda Pires; Cavallini,Danilo.
ABSTRACT Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis. According to the minimum detection limit curve (in CT), REP-529 had greater sensitivity to detect T. gondii DNA than B1. Both primer sets were retrospectively evaluated using 515...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Symptomatic toxoplasmosis; Diagnosis; Real-time polymerase chain reaction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600638

Comparison of conventional methods for diagnosis of visceral leishmaniasis in children of the Center-West Region of Brazil
Provedor de dados: 55
Autores: Brustoloni,Yvone M.; Cunha,Rivaldo V.; Dorval,Maria Elizabeth; Oshiro,Elisa T.; Pontes,Elenir R.J.C.; Oliveira,Ana Lúcia L.; Hillebrand,Lilian; Ribeiro,Luis Felipe.
In Brazil, sophisticated techniques currently employed for diagnosis of visceral leishmaniasis, such as polymerase chain reaction-based assays, are only available in major research centers, whereas conventional methods are still used in many areas where the disease occurs. In the state of Mato Grosso do Sul, in the country's Center-West Region, visceral leishmaniasis has recently emerged in many cities, and duration of the disease, from the onset of symptoms to diagnosis, has been short. Considering that results of diagnostic tests may depend on the phase of the disease, we compared direct examination of bone marrow aspirates (BMAs), BMA culture, and serology by Indirect Immunofluorescence Antibody Test (IFAT) for diagnosis in children, according to time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Kala-azar; Diagnosis; Direct microscopy; Immunological tests.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100023

Diagnosis of pulmonary tuberculosis by score system in children and adolescents: a trial in a reference center in Bahia, Brazil
Provedor de dados: 55
Autores: Sant'Anna,Clemax Couto; Santos,Marilene Augusta R. C.; Franco,Rosana.
Since 2002, the Brazilian Ministry of Health has recommended a score system for tuberculosis diagnosis of children and adolescents that does not need bacteriological positivity, because most cases in this age group have few bacteria. An observational, transversal study was carried out at the outpatient health care service of the reference medical service in Salvador, Bahia, including 164 patients with pulmonary tuberculosis, with ages ranging between 1 and 15 years of age, who were treated from 1990 to 2001. The gold standard used to establish the diagnosis was clinical, radiological, epidemiological and based on follow-up data. The score system for diagnosis purposes was tested retrospectively. The median age and the average age of the 164 patients were 6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary tuberculosis; Diagnosis; Child; Adolescents.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000400006

Urine as a promising sample for Leishmania DNA extraction in the diagnosis of visceral leishmaniasis - a review
Provedor de dados: 55
Autores: Bezerra,Gilberto Silva Nunes; Barbosa Júnior,Walter Lins; Silva,Elis Dionísio da; Leal,Nilma Cintra; Medeiros,Zulma Maria de.
ABSTRACT Visceral leishmaniasis is a serious and debilitating infection with high fatality rate in tropical and subtropical countries. As clinical symptoms of visceral leishmaniasis are not so specific, confirmatory diagnostic methods with high sensitivity and specificity are needed. Noninvasive methods have been developed using urine as a clinical sample for visceral leishmaniasis diagnosis. In fact, there is a clear correlation between kidney impairment and Leishmania DNA in urine. However, it has been proved that Leishmania nucleic acid may also be isolated from patients without any sign of renal involvement. Even though urine has become a promissing biological sample, it is still not widely used due to several issues, such as (i) incomprehension of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Renal involvement; Diagnosis; Urine sample; DNA extraction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200111

Accuracy of a self-collection kit for the microbiological study of the vaginal content
Provedor de dados: 55
Autores: Passos,Mauro Romero L.; Varella,Renata Q.; Barreto,Nero A.; Garcia,Maria Luiza; Giraldo,Paulo C.
Diagnosis of vaginal discharge is frequently performed in an empirical way, leading to inadequate treatment. This study tested the accuracy of a self-collection kit for microbiological study of the vaginal content. One hundred and forty-two women of Family Health Program units in Niterói and Piraí cities were enrolled in order to have their vaginal content studied. A brief explanation and a self-collection kit were provided in order to sample the vaginal content. The self-collection kit was composed of one empty plastic tube, two glass slides, a long handle cytobrush, an identification card and guideline notes. The vaginal sample was applied on the glass slides by the women and stained by Gram technique. A second sampling was done by the medical personnel....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Self-collection kit; Vaginitis; Diagnosis; Vaginal discharge; Bacterial vaginosis; Vaginal flora.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200017

Diagnosis of American cutaneous leishmaniasis by enzyme immunoassay in patients from Northern Paraná State, Brazil
Provedor de dados: 55
Autores: Yoneyama,Kelly Aparecida Geraldo; Peder,Leyde Daiane de; Lonardoni,Maria Valdrinez Campana; Silveira,Thaís Gomes Verzignassi.
American cutaneous leishmaniasis (ACL) is an endemic disease caused by Leishmania parasites. The ACL diagnosis is commonly accomplished by parasitological and immunological methods. The objective of this work was to evaluate the enzyme immunoassay (EIA) for ACL laboratorial diagnosis. IgG antibodies against Leishmania (Viannia) braziliensis promastigotes were researched. For the method standardization 240 sera were used: 72 from patients with positive parasitological diagnosis, 38 from normal individuals and 113 from individuals with other pathologies. The sensibility was 93% and the specificity was 70%. Regarding diagnosis, EIA showed 97% positivity in patients with ACL and 97% negativity in patients with other cutaneous lesions. The EIA presented a good...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Diagnosis; Antibodies; Immunoenzyme; Leishmania braziliensis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300012

Diagnostic parameters and reliability of four rapid immunochromatographic tests for dengue 4
Provedor de dados: 55
Autores: Mata,Veronica Elizabeth; Passos,Sonia Regina Lambert; Santos,Maria Angelica Borges dos; Buonora,Sibelle Nogueira; de Andrade,Carlos Augusto Ferreira; Lima,Monique da Rocha Queiroz; Costa,Betina Moreira; Hökerberg,Yara Hahr Marques.
ABSTRACT  Background: Although performance of rapid immunochromatographic tests (RITs) for dengue virus (DENV) serotypes 1, 2 and 3 is relatively settled, evidence on accuracy of RITs for DENV-4 are based on studies with small sample sizes and with discrepant results.  Objectives: To assess accuracy and inter-observer agreement of RITs targeting dengue nonstructural protein-1 (NS1) antigen - Dengue NS1-Bioeasy™, Dengue NS1 Ag Strip-Bio-Rad™, IVB Dengue Ag NS1-Orangelife™ and Dengue NS1-K130-Bioclin™ in DENV-4 samples.  Methods: Study sample (n = 324) included adults presenting at an emergency unit in Rio de Janeiro, Brazil, with fever of ≤72 h and two or more dengue symptoms. A serum sample from each patient was tested by each RIT. A positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dengue; Immunochromatographic tests; Sensitivity and specificity; Reproducibility of tests; Diagnosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100058

Evaluation of an enzyme-linked immunospot assay for the immunodiagnosis of atypical spinal tuberculosis (atypical clinical presentation/atypical radiographic presentation) in China
Provedor de dados: 55
Autores: Yuan,Kai; Zhong,Zhao-ming; Zhang,Qiang; Xu,Shu-chai; Chen,Jian-ting.
BACKGROUND: Atypical spinal tuberculosis (TB) usually presents in a slowly indolent manner with nonspecific clinical presentations making the diagnosis a great challenge for physicians. New technologies for the detection of atypical spinal TB are urgently needed. The aim of this study was to assess the diagnostic value of an enzyme-linked immunospot (ELISPOT) assay in clinically suspected cases of atypical spinal TB in China. METHODS: From March 2011 to September 2012, a total of 65 patients with suspected atypical spinal TB were enrolled. In addition to conventional tests for TB, we used ELISPOT assays to measure the IFN-I response to ESAT-γ and CFP-10 in T-cells in samples of peripheral blood mononuclear cells. Patients with suspected atypical spinal TB...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atypical spinal tuberculosis; Enzyme-linked immunospot assay; T-SPOT.TB kit; Diagnosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000500004

Bone mineral density and vitamin D concentration: the challenges in taking care of children and adolescents infected with HIV
Provedor de dados: 55
Autores: Carmo,Fabiana Bononi; Terreri,Maria Teresa; Succi,Regina Célia de Menezes; Beltrão,Suenia Vasconcelos; Gouvea,Aida de Fátima Tomé Barbosa; Paulino,Erica Regina Cruz; Machado,Daisy Maria.
ABSTRACT  Background: The increase in life expectancy for patients living with human immunodeficiency virus (HIV) infection has resulted in health complications related to a chronic disease.  Objectives: To evaluate the prevalence of bone mineral density (BMD) alterations and vitamin D concentrations in HIV-infected children and adolescents and to verify the variations in those parameters during a 12-month interval.  Methods: A prospective cohort study with a dual period of evaluation was conducted in 57 patients perinatally HIV-infected and one patient with sexual abuse in early infancy. Demographic, anthropometric, pubertal stage, viral load, T CD4+ cell count and antiretroviral therapy were evaluated. Biochemical tests and total body (TB) and lumbar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Antiretroviral; Infant; Diagnosis; Metabolism; Bone.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300270

Comparative performances of serologic and molecular assays for detecting human T lymphotropic virus type 1 and type 2 (HTLV-1 and HTLV-2) in patients infected with human immunodeficiency virus type 1 (HIV-1)
Provedor de dados: 55
Autores: Campos,Karoline Rodrigues; Gonçalves,Maria Gisele; Costa,Nadia Aparecida; Caterino-de-Araujo,Adele.
ABSTRACT The present study evaluated several techniques currently available (commercial kits and in-house assays) for diagnosing human T lymphotropic viruses types 1 and 2 in two groups of patients enrolled at HIV/AIDS specialized care services in São Paulo: Group 1 (G1), n = 1608, 1237 male/371 female, median age 44.3 years old, majority using highly active antiretroviral therapy (HAART); G2, n = 1383, 930 male/453 female, median age of 35.6 years old, majority HAART naïve. Enzyme immunoassays [(EIA) Murex and Gold ELISA] were employed for human T lymphotropic viruses types 1 and 2 screening; Western blotting (WB), INNO-LIA (LIA), real-time PCR pol (qPCR), and nested-PCR-RFLP (tax) were used to confirm infection. Samples were considered human T...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HTLV-1/2; HIV-1; Coinfection; Diagnosis; Immunoblotting; PCR; HAART.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300297

Successive mycological nail tests for onychomycosis: a strategy to improve diagnosis efficiency
Provedor de dados: 55
Autores: Meireles,Tereza Elizabeth Fernandes; Rocha,Marcos Fábio Gadelha; Brilhante,Raimunda Sâmia Nogueira; Cordeiro,Rossana de Aguiar; Sidrim,José Júlio Costa.
Onychomycosis is a fungal infection of nails caused by dermatophytes, yeasts and moulds, accounting for about 50% of onychopathies. A high frequency of onychomycosis caused by Candida species has been reported during the last few years in northeast Brazil, as well as in other regions of the world. A clinical diagnosis of onychomycosis needs to be confirmed through laboratory exams. We evaluated the importance of serial repetition of direct microscopic exams and fungal culture for the diagnosis of onychomycosis in the city of Fortaleza, Ceará, in northeast Brazil. We first made a retrospective study of 127 patients with onychomycosis, identifying the fungi that had been isolated from fingernails and toenails. We then made a prospective study of 120...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Onychomycosis; Candida; Moulds and dermatophytes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400016

Role of the Parasight-F test in the diagnosis of complicated Plasmodium falciparum malarial infection
Provedor de dados: 55
Autores: Arora,Sandeep; Gaiha,Manorama; Arora,Anju.
An evaluation was made of the diagnostic efficacy and utility of the Parasight-F test in diagnosing Plasmodium falciparum malaria, compared with conventional microscopy, particularly in severe and complicated cases. This study was designed as a prospective, case control hospital-based study. Febrile patients suspected to be suffering from malaria were selected randomly and were subjected to peripheral smear examinations (thick and thin) and Parasight-F tests till the required number of at least 30 cases of P. falciparum infection were identified, including at least 15 complicated cases. In addition 20 cases of P. vivax malarial infection as well as 20 healthy age and sex-matched individuals were taken as two control groups. The outcome measure was the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malaria; Diagnosis; Plasmodium falciparum; Parasight-F test.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000500008

Galactomannan use in clinical practice: providing free testing is not the full answer
Provedor de dados: 55
Autores: dos Santos,Jeniffer S.; Hermes,Djuli M.; Pasqualotto,Alessandro C.
ABSTRACT  Introduction: Invasive aspergillosis is a condition associated with a high mortality rate mostly due to difficulties in performing an early diagnosis. In recent years, galactomannan detection has markedly improved the diagnosis of invasive aspergillosis, but very little is known on how physicians deal with this test in clinical practice.  Methods: This cross-sectional study aimed to analyze the indications for the use of serum galactomannan in a large Brazilian hospital, between 2015 and 2016. No specific protocol was in place for GM request. We reviewed the medical records of adult (&gt;18 years-old) patients who were tested for galactomannan due to one the following indications: screening, diagnosis, or treatment follow-up. Additional variables...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus; Aspergillosis; Opportunistic infections; Diagnosis; Tomography; Neutropenia; Immunosuppressed.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000100037

Diagnosis of human papillomatosis by polymerase chain reaction in cases of divergence between results of hybrid capture and papanicolaou cytology
Provedor de dados: 55
Autores: Novaes,Luiz Carlos Garcez; Novaes,Maria Rita Carvalho Garbi; Simões-Barbosa,Augusto.
As various types of human papillomavirus (HPV) are involved in the pathogenesis of cervical cancer, correct diagnosis is of fundamental importance for screening programs. We evaluated the divergence of results between Papanicolaou cytology and hybrid capture by PCR detection of HPV DNA . A transversal study was conducted on 70 women attending private gynecological clinics in Brasilia, Brazil. PCRs were conducted with specific primers for general and high-risk HPV DNA. Based on the PCR results, hybrid capture was a superior diagnostic technique. When Papanicolaou was compared with the molecular biology methods, it was found that a positive Papanicolaou result does not necessarily indicate the presence of HPV. The agreement between PCR and hybrid capture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; Diagnosis; Hybrid capture; PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000300003

Infection by Mycoplasma pneumoniae and its importance as an etiological agent in childhood community-acquired pneumonias
Provedor de dados: 55
Autores: Vervloet,Letícia Alves; Marguet,Christophe; Camargos,Paulo Augusto Moreira.
This manuscript reviewed the literature on infection by Mycoplasma pneumoniae with emphasis on etiological aspects of childhood community-acquired pneumonias. Bibliographical research was carried out from Pubmed Medline, MDConsult, HighWire, LILACS, and direct research over the past 10 years with the following keywords: Mycoplasma pneumoniae, pneumonia, and childhood. Fifty-four articles were selected. Mycoplasma pneumoniae has a high incidence in childhood. Clinical presentation includes respiratory and extrarespiratory symptoms. Mycoplasma pneumoniae lung infection can be confused with viral or bacterial pneumonia and is unresponsive to beta-lactams. In addition, co-infections have been reported. Mycoplasma pneumoniae infection occurs in all age groups,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma pneumoniae; Pneumonia; Extrapulmonary findings; Diagnosis; Treatment.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500012

Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples
Provedor de dados: 55
Autores: Macedo,Carla Isabel; Carnieli Jr,Pedro; Brandão,Paulo Eduardo; Rosa,Elizabeth S. Travassos da; Oliveira,Rafael de Novaes; Castilho,Juliana Galera; Medeiros,Rita; Machado,Rosangela Rocha; Oliveira,Rosely Cerqueira de; Carrieri,Maria Luiza; Kotait,Ivanete.
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human rabies; Neck-skin; Diagnosis; RT-PCR; DNA sequencing.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008

Combined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis
Provedor de dados: 55
Autores: Beck,S. T.; Leite,O. M.; Arruda,R. S.; Ferreira,A. W.
Two recombinant antigens and a crude bacterial antigen of a wild M. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. The patients were not infected with HIV. We evaluated the sensitivity and specificity of ELISA, based on the recombinant TbF6® and TbF6/DPEP antigen and a search for reactivity patterns in the Western blot technique, using whole mycobacterium antigen. Serum samples from 22 healthy individuals and from 30 patients with lung diseases other than tuberculosis were used as controls. The best ELISA results were obtained with the TbF6/DPEP antigen combination, which gave 85% sensitivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: M. tuberculosis; Recombinant antigens; Diagnosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100007

Validity of the polymerase chain reaction in the diagnosis of clinically suspected cases of American visceral leishmaniasis
Provedor de dados: 55
Autores: Pedrosa,Celia Maria Silva; Ximenes,Ricardo Arraes de Alencar; Almeida,Wendell Alexandre Pinheiro de; Rocha,Eliana Maria Mauricio da.
To test the validity of the polymerase chain reaction for diagnosing American visceral leishmaniasis, 88 suspected cases were studied. Diagnosis was confirmed in 47 (53.5%) and ruled out in 41 (46.5%) patients. Samples of bone marrow and peripheral blood were processed by polymerase chain reaction to evaluate the sensitivity and specificity of the test and its agreement beyond chance with microscopy examination. The polymerase chain reaction was positive in bone marrow of 100% of the patients with amastigotes seen with microscopy examination, and in 59.5% in those where no parasite were seen. Agreement beyond chance between visualization of the parasite in bone marrow aspirates and polymerase chain reaction was considered weak (Kappa = 0.41). Concordance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: American visceral leishmaniasis; Diagnosis; Polymerase chain reaction; Peripheral blood; Bone marrow blood.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300003

Importance of high IgG anti-Toxoplasma gondii titers and PCR detection of T. gondii DNA in peripheral blood samples for the diagnosis of AIDS-related cerebral toxoplasmosis: a case-control study
Provedor de dados: 55
Autores: Vidal,José E; Diaz,Adrián Vladimir Hernández; Oliveira,Augusto César Penalva de; Dauar,Rafi Felicio; Colombo,Fabio Antonio; Chioccola,Vera Lucia Pereira.
BACKGROUND: Cerebral toxoplasmosis (CT) continues to cause significant morbidity and mortality in human immunodeficiency virus (HIV)-infected patients in Brazil. In clinical practice, the initial diagnosis is usually presumptive and alternative diagnosis tools are necessary. Our objective was to evaluate whether the detection of high titers of IgG anti-Toxoplasma gondii and T. gondii DNA in blood samples are associated with the diagnosis of CT. METHODS: In this case-control study we included 192 patients with HIV-1 infection: 64 patients with presumptive CT (cases) and 128 patients with other diseases (controls). Blood samples to perform indirect immunofluorescense reaction (IFI) to detect anti-T. gondii IgG antibodies and polymerase chain reaction (PCR)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; Cerebral toxoplasmosis; Polymerase chain reaction; Diagnosis; Acquired immunodeficiency syndrome; Brazil.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400009

Detection of multidrug-resistant Mycobacterium tuberculosis strains isolated in Brazil using a multimarker genetic assay for katG and rpoB genes
Provedor de dados: 55
Autores: Café Oliveira,Luita Nice; Muniz-Sobrinho,Jairo da Silva; Viana-Magno,Luiz Alexandre; Oliveira Melo,Sônia Cristina; Macho,Antonio; Rios-Santos,Fabrício.
Abstract Multidrug-resistant tuberculosis (MDRTB) is a serious world health problem that limits public actions to control tuberculosis, because the most used anti-tuberculosis first-line drugs fail to stop mycobacterium spread. Consequently, a quick detection through molecular diagnosis is essential to reduce morbidity and medical costs. Despite the availability of several molecular-based commercial-kits to diagnose multidrug-resistant tuberculosis, their diagnostic value might diverge worldwide since Mycobacterium tuberculosis genetic variability differs according to geographic location. Here, we studied the predictive value of four common mycobacterial mutations in strains isolated from endemic areas of Brazil. Mutations were found at the frequency of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; Diagnosis; Multidrug-resistance; Mycobacterium tuberculosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200166

Diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus in Latin America
Provedor de dados: 55
Autores: Zurita,Jeannete; Mejía,Carlos; Guzmán-Blanco,Manuel.
Strategies to monitor and control the spread of methicillin-resistant Staphylococcus aureus (MRSA) infections are dependent on accurate and timely diagnosis of MRSA in both hospital and community settings. In Latin America, significant diversity in diagnostic and susceptibility testing procedures exists at the regional, national and local levels. Various tests for S. aureus and MRSA are available in clinical settings, but the application of these techniques differs between and within countries, and quality control measures are not uniformly applied to verify diagnoses. To optimize the diagnosis of MRSA infections across Latin America, a more consistent approach is required. This may include: adoption and appropriate adaption of specific guidelines for MRSA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Diagnosis; Susceptibility testing; Latin America.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800005

Multidrug-resistant tuberculosis
Provedor de dados: 55
Autores: Lemos,Antônio Carlos Moreira; Matos,Eliana Dias.
Despite the efforts made worldwide to reduce the number of cases of drug-susceptible tuberculosis, multidrug-resistant tuberculosis (MDR-TB) constitutes an important public health issue. Around 440,000 new cases of MDR-TB are estimated annually, although in 2008 only 7% of these (29,423 cases) were notified. The laboratory tests for diagnosing resistance may be phenotypic (based on culture growth in the presence of drugs) or genotypic (i.e. identification of the presence of mutations that confer resistance). The urgent need for a rapid means of detecting resistance to anti-TB drugs has resulted in the development of many genotypic methods over recent years. The treatment of MDR-TB is expensive, complex, prolonged (18-24 months) and associated with a higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multidrug-resistant tuberculosis; Review; Diagnosis; Treatment.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200018

Radiologic findings of pulmonary tuberculosis in adolescents
Provedor de dados: 55
Autores: Sant'Anna,Clemax Couto; Schmidt,Christianne Mello; March,Maria de Fátima B Pombo; Pereira,Susan Martins; Barreto,Maurício Lima.
OBJECTIVE: To describe radiologic findings of pulmonary tuberculosis (TB) in adolescents. METHODS: Retrospective, cross-sectional, observational study of 850 patients with TB, aged 10 to 19 years, and notified to the Brazilian Ministry of Health. Data were collected from the TB notification and medical records in the cities of Manaus, Amazonas State, and Salvador, Bahia State, in the 19962003 period. Data are shown in tables and analyzed using the chi-square and Mann-Whitney tests, with a 5% significance level. RESULTS: Mean age was 15.6 years; 443 (52.1%) patients were males. The most common radiologic lesion was the upper pulmonary lobe infiltrate (53.3%), and isolated cavitation was found in 32.4% of the patients. Both lungs were affected in 29.2% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Adolescents; Diagnosis; Chest radiography.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100008

Dengue: a review of the laboratory tests a clinician must know to achieve a correct diagnosis
Provedor de dados: 55
Autores: De Paula,Sérgio Oliveira; Fonseca,Benedito Antônio Lopes da.
Dengue is the most important disease caused by an arbovirus (1, 2, 3 and 4 serotypes) worldwide, especially in the tropical and sub-tropical regions. Its clinical manifestations range from asymptomatic infections to a severe disease characterized by hemorrhage and shock. The incidence of dengue virus activity in the Americas has substantially increased from 1980 to 1994. In Brazil, the increase in the incidence of dengue is especially linked to the dissemination of Aedes aegypti. Thus, a rapid and accurate dengue diagnosis is of paramount importance for effective control of dengue outbreaks [8]. Five serological tests have been used for the diagnosis of dengue infection: hemagglutination-inhibition (HI), complement fixation (CF), neutralization test (NT),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dengue; Diagnosis; Serology; PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000600002

Pulmonary cryptococcosis in non-AIDS patients
Provedor de dados: 55
Autores: ,Jin-Quan; Tang,Ke-Jing; Xu,Bing-Ling; Xie,Can-Mao; Light,Richard W.
OBJECTIVE: To investigate the clinical features, management, and prognosis of pulmonary cryptococcosis in non-acquired immunodeficiency syndrome (AIDS) patients. METHOD: 24 cases of pulmonary cryptococcosis with accurate pathological diagnosis were retrospectively studied. RESULTS: 15 male patients and nine female patients were diagnosed at the first affiliated hospital of Sun Yat-sen University from November 1999 to November 2011. The mean age at the time of diagnosis was 44.2 ± 11.3 years (range: 24 to 65 years). Among these patients, 13 had other comorbidities. 15 were symptomatic and the other nine were asymptomatic. The most common presenting symptoms were cough, chest tightness, expectoration, and fever. None had concurrent cryptococcal meningitis....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary cryptococcosis; Clinical presentation; Diagnosis; Treatment; HIV negative.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600006

Hepatitis C virus core antigen assay: can we think beyond convention in resource limited settings?
Provedor de dados: 55
Autores: Chakravarti,Anita; Chauhan,Mayank S.; Dogra,Gaurav; Banerjee,Sayantan.
ABSTRACT Hepatitis C virus infects over 15 million patients from India and 2.86 million from Brazil. Detection of anti-hepatitis C virus antibodies has limited sensitivity during acute phase: the pre-seroconversion window period. Hepatitis C virus-RNA detection techniques are used to overcome this shortfall, but are costly and unavailable widely in developing countries. Estimation of hepatitis C virus core-antigen, a protein with highly conserved sequence, by enzyme-immunoassays is an economic and simpler alternative to RNA detection. This study was conducted in Delhi, involving 300 acute and chronic liver disease patients, tested for anti-hepatitis C virus 3rd-generation ELISA, hepatitis C virus core-antigen-ELISA and hepatitis C virus-RNA reverse...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis C; Diagnosis; Core antigen; HCVcAg.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300010

Evaluation of the rapid diagnostic test OptiMAL for diagnosis of malaria due to Plasmodium vivax
Provedor de dados: 55
Autores: Tarazona,Alonso Soto; Zerpa,Lely Solari; Mendoza Requena,Daniel; Llanos-Cuentas,Alejandro; Magill,Allan.
OBJECTIVE: To determine the sensitivity and specificity of the rapid diagnostic test OptiMAL® for diagnosis of Plasmodium vivax malaria. MATERIAL AND METHODS: We included all the patients who sought medical attention in the San Martin Pangoa Hospital, Junin, an area endemic for vivax malaria in Peru, between October and December 1998, who had fever during the previous 72 hours and who were older than 12 months. The gold standard for diagnosis was thick blood film microscopy. We determined the parasitemia rate for each of the positive slides. We calculated sensitivity, specificity, positive predictive value and negative predictive value of the test. RESULTS: We included 72 patients; 39 of them were positive for P. vivax by microscopic examination. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: OptiMAL; Malaria; Plasmodium vivax; Diagnosis; Dipstick.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000200005

Infectious keratitis secondary to Histoplasma capsulatum: the first case reports in humans
Provedor de dados: 55
Autores: Arcieri,Enyr S.; Rocha,Ademir; Mendonça,Camila N.; Andreo,Eduardo G. V.; Finotti,Ioná G.A.; Furlanetto,Rafael L.; Arcieri,Rafael S.; Rocha,Flávio J.; Rizzo,Luiz V.
The authors report an unusual case of fungal keratitis caused by Histoplasma capsulatum in a male immunocompetent patient. PCR confirmed the presence of the fungus DNA in the material studied. To our knowledge this is the first reported case in humans described all over the world.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human; Keratitis; Histoplasma; Diagnosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000600013

Polymerase chain reaction as a useful and simple tool for rapid diagnosis of tuberculous meningitis in a Brazilian tertiary care hospital
Provedor de dados: 55
Autores: Dora,José Miguel; Geib,Guilherme; Chakr,Rafael; Paris,Fernanda de; Mombach,Alice Beatriz; Lutz,Larissa; Souza,Carolina Fischinger Moura de; Goldani,Luciano Z.
Meningitis is a severe and potentially fatal form of tuberculosis. The diagnostic workup involves detection of acid-fast bacilli (AFB) in the cerebrospinal fluid (CSF) by microscopy or culture, however, the difficulty in detecting the organism poses a challenge to diagnosis. The use of the polymerase chain reaction (PCR) in the diagnostic approach to Mycobacterium tuberculosis (MTB) meningitis has been reported as a fast and accurate method, with several commercial kits available. As an alternative, some institutions have been developing inexpensive in house assays. In our institution, we use an in house PCR for tuberculosis. We analyzed the performance of our PCR for the diagnosis of MTB meningitis in 148 consecutive patients, using MTB culture as gold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculous meningitis; Diagnosis; Polymerase chain reaction; PCR; Mycobacterium tuberculosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300015

Disseminated histoplasmosis in HIV positive patients in Espírito Santo state, Brazil: a clinical-laboratory study of 12 cases (1999-2001)
Provedor de dados: 55
Autores: Casotti,Janaina Aparecida Schineider; Motta,Tânia Queiroz Reuter; Ferreira Júnior,Carlos Urbano Gonçalves; Cerutti Junior,Crispim.
Twelve cases of histoplasmosis in HIV-infected patients were found in a retrospective analysis at the Hospital Universitário Cassiano Antônio de Moraes of the Universidade Federal do Espírito Santo (HUCAM), Vitória (ES), from June 1999 to May 2001. The frequency of histoplasmosis among HIV-positive patients was 2.1% in the infectious diseases division of the hospital during this period. Histoplasmosis compromised mainly males (11/12), 27 to 44 years old, and residents of the metropolitan urban area (10/12). Alcohol abuse and tobacco smoking were described in 10 of the 12 patients. For all patients, this was the first opportunistic infection. Two of the 12 patients died; 10 patients had disseminated disease, one patient had an intestinal presentation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histoplasmosis; AIDS; Diagnosis; Case series.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500005

Strategies to reduce mortality and morbidity due to AIDS-related cryptococcal meningitis in Latin America
Provedor de dados: 55
Autores: Vidal,Jose E.; Oliveira,Augusto C. Penalva de; Dauar,Rafi F.; Boulware,David R.
Latin America is the region with the third most AIDS-related cryptococcal meningitis infections globally. Highly active antiretroviral therapy (HAART) has reduced the number of infections; however, the number of deaths and the case-fatality rate continues to be unacceptable. In this review, we focus on the burden of AIDS-related cryptococcosis in Latin America and discuss potential strategies to reduce early mortality from Cryptococcus. In this review, we highlight the importance of: (1) earlier HIV diagnosis and HAART initiation with retention-in-care to avoid AIDS; (2) pre-HAART cryptococcal antigen (CRAG) screening with preemptive fluconazole treatment; (3) better diagnostics (e.g. CRAG testing); and (4) optimal treatment with aggressive management of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcal meningitis; Cryptococccosis; Epidemiology; Diagnosis; Treatment; Mortality; Latin America.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300008

Alteration in the erythrocyte sedimentation rate in dengue patients: analysis of 1,398 cases
Provedor de dados: 55
Autores: Souza,Luiz José de; Reis,Aldo Franklin Ferreira; Almeida,Fernanda Carlos Rodrigues de; Souza,Lara Assed de; Abukater,Marina; Gomes,Maurício Assed Estefan; Abicair,Olívia Araujo; Gonçalves,Pedro Assed.
A study of the erythrocyte sedimentation rate during the first hour (ESR) in dengue patients would help determine how this parameter is affected by this disease, as well as whether it can be used for diagnosis. One thousand, three hundred and ninety-eight cases of dengue attended at the Dengue Treatment Center, Campos dos Goytacazes Rio de Janeiro, Brazil, were included. The ESR values were classified as normal or elevated and compared by gender and clinical form of the disease. Among the 1,398 cases ESR was normal in 81.25% (n = 1,136), while in 18.75% (n=262) it was elevated. In 83.92% (n=514) of the male dengue patients ESR was normal, while in 16.08% (n=98) it was elevated. Among female dengue patients, 79.17% (n=622) had normal ESR, and in 20.83%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dengue fever; Erythrocyte sedimentation rate (ESR); Diagnosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600005

Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil
Provedor de dados: 55
Autores: Santos,Fred Luciano Neves; Gonçalves,Marilda de Souza; Soares,Neci Matos.
Amoebiasis is an infection caused by Entamoeba histolytica and is a potential health risk in countries in which health barriers are inappropriate. Since the discovery of Entamoeba dispar, the prevalence of amoebiasis has been modified. OBJECTIVE: This study has standardized the PCR technique applied for the diagnosis of different species of the E. histolytica/E. dispar complex and has evaluated the prevalence of infection among patients attending private and public clinical laboratories in Salvador City, Bahia State, Brazil. RESULTS: Analysis of 52,704 stool samples by microscopic examination demonstrated that 1,788 (3.4%) were positive for the E. histolytica/E. dispar complex and infection occurred more often in samples originated from public clinical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amebiasis; Entamoeba histolytica; Diagnosis; Prevalence.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200005

Clinical signs of pneumonia in children: association with and prediction of diagnosis by fuzzy sets theory
Provedor de dados: 56
Autores: Pereira,J.C.R.; Tonelli,P.A.; Barros,L.C.; Ortega,N.R.S.
The present study compares the performance of stochastic and fuzzy models for the analysis of the relationship between clinical signs and diagnosis. Data obtained for 153 children concerning diagnosis (pneumonia, other non-pneumonia diseases, absence of disease) and seven clinical signs were divided into two samples, one for analysis and other for validation. The former was used to derive relations by multi-discriminant analysis (MDA) and by fuzzy max-min compositions (fuzzy), and the latter was used to assess the predictions drawn from each type of relation. MDA and fuzzy were closely similar in terms of prediction, with correct allocation of 75.7 to 78.3% of patients in the validation sample, and displaying only a single instance of disagreement: a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiologic methods; Stochastic models; Fuzzy models; Clinical signs; Diagnosis; Data analysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500012

Serum cytokine levels in autoimmune and non-autoimmune hyperthyroid states
Provedor de dados: 56
Autores: Ward,L.S.; Fernandes,G.A.
Although the role of interleukin-2 (IL-2) and interferon gamma (&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;IFN) is still poorly understood in hyperthyroid diseases, it is reasonable to assume that these cytokines may be present at higher levels in Graves' disease (GD) than in other primarily non-autoimmune thyroid diseases. In order to look for an easy method to distinguish GD from primarily non-autoimmune causes of hyperthyroidism, we compared 13 healthy individuals with 21 treated and untreated hyperthyroid GD patients and with 19 patients with hyperthyroidism due to other etiologies: 7 cases of multinodular goiter, 5 cases of excessive hormone replacement and 7 cases of amiodarone-associated hyperthyroidism. All patients presented low TSH levels and a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytokines; IL-2; Hyperthyroidism; Graves' disease; Diagnosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100009

Expression and purification of the non-tagged LipL32 of pathogenic Leptospira
Provedor de dados: 56
Autores: Hauk,P.; Carvalho,E.; Ho,P.L.
Leptospirosis is a reemerging infectious disease and the most disseminated zoonosis worldwide. A leptospiral surface protein, LipL32, only occurs in pathogenic Leptospira, and is the most abundant protein on the bacterial surface, being described as an important factor in host immunogenic response and also in bacterial infection. We describe here an alternative and simple purification protocol for non-tagged recombinant LipL32. The recombinant LipL32(21-272) was expressed in Escherichia coli without His-tag or any other tag used to facilitate recombinant protein purification. The recombinant protein was expressed in the soluble form, and the purification was based on ion exchange (anionic and cationic) and hydrophobic interactions. The final purification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigen; LipL32; Pathogenic Leptospira; Non-tagged protein purification; Diagnosis; Vaccine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400005

Diagnostic value of circulating lncRNA ANRIL and its correlation with coronary artery disease parameters
Provedor de dados: 56
Autores: Hu,Yao; Hu,Jing.
This study aimed to detect the expression of the long non-coding RNA (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) and evaluate its correlation with disease risk, stenosis degree, inflammation, as well as overall survival (OS) in coronary artery disease (CAD) patients. A total of 230 patients who underwent diagnostic coronary angiography were consecutively recruited and assigned to CAD group (n=125) or control group (n=105) according to presence or absence of CAD. Gensini score was calculated to assess the severity of coronary artery damage. Plasma samples were collected and the expression ANRIL was detected in all participants. High-sensitivity C-reactive protein (hs-CRP), erythrocyte sedimentation rate (ESR), and cytokines including tumor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA ANRIL; Coronary artery disease; Diagnosis; Disease severity; Inflammatory responses; Overall survival.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800612

The efficacy of YKL-40 and CA125 as biomarkers for epithelial ovarian cancer
Provedor de dados: 56
Autores: Zou,L.; He,X.; Zhang,J.W.
Our objective was to estimate the efficacy of the measurement of serum YKL-40 alone or with CA125 as biomarkers for the diagnosis of epithelial ovarian cancer (EOC) using the YKL-40 ELISA kit. An experimental group of 49 ovarian cancer patients included 42 patients with EOC (53 ± 15 years, range: 19-81 years) and 7 patients (48 ± 13 years, range: 29-36 years) with borderline epithelial ovarian tumor. A control group of 88 non-malignant cases included 42 patients (43 ± 10 years, range: 26-77 years) with benign gynecological disease and 46 healthy women (45 ± 14 years, range: 30-68 years) at a teaching hospital. Both YKL-40 (220.1 ± 94.1 vs 61.6 ± 48.4 and 50.1 ± 41.2 ng/mL) and CA125 (524.9 ± 972.5 vs 13.4 ± 7.6 and 28.5 ± 29.6 U/mL) levels were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YKL-40; CA125; Ovarian cancer; Diagnosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200013

Fluorescent quantitative PCR of Mycobacterium tuberculosis for differentiating intestinal tuberculosis from Crohn's disease
Provedor de dados: 56
Autores: Fei,B.Y.; Lv,H.X.; Zheng,W.H.
Intestinal tuberculosis (ITB) and Crohn's disease (CD) are granulomatous disorders with similar clinical manifestations and pathological features that are often difficult to differentiate. This study evaluated the value of fluorescent quantitative polymerase chain reaction (FQ-PCR) for Mycobacterium tuberculosis (MTB) in fecal samples and biopsy specimens to differentiate ITB from CD. From June 2010 to March 2013, 86 consecutive patients (38 females and 48 males, median age 31.3 years) with provisional diagnoses of ITB and CD were recruited for the study. The patients' clinical, endoscopic, and histological features were monitored until the final definite diagnoses were made. DNA was extracted from 250 mg fecal samples and biopsy tissues from each patient....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal tuberculosis; Crohn's disease; Diagnosis; Fluorescent quantitative PCR; Mycobacterium tuberculosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200166

Non-invasive messenger RNA transcriptional evaluation in human kidney allograft dysfunction
Provedor de dados: 56
Autores: Joelsons,G.; Domenico,T.; Gonçalves,L.F.; Manfro,R.C.
The aim of the present study was to evaluate messenger RNA expression in kidney allograft recipients. Forty-four kidney transplant recipients were evaluated up to three months after grafting. After transplantation, peripheral blood samples were drawn sequentially for real-time polymerase chain reaction analyses of perforin and TIM-3 genes. Biopsies were obtained to evaluate acute graft dysfunction and interpreted according to the Banff classification. Eight patients presented episodes of acute rejection. Recipients with rejection had significantly higher levels of TIM-3 mRNA transcripts compared to those without rejection (median gene expression 191.2 and 36.9 mRNA relative units, respectively; P&lt;0.0001). Also, perforin gene expression was higher in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney transplantation; Acute rejection; Gene expression; Diagnosis; MRNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700608

Expression of a hantavirus N protein and its efficacy as antigen in immune assays
Provedor de dados: 56
Autores: Figueiredo,L.T.M.; Moreli,M.L.; Borges,A.A.; Figueiredo,G.G.; Souza,R.L.M.; Aquino,V.H.
Hantavirus cardiopulmonary syndrome (HCPS) has been recognized as an important public heath problem. Five hantaviruses associated with HCPS are currently known in Brazil: Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos, and Anajatuba viruses. The laboratory diagnosis of HCPS is routinely carried out by the detection of anti-hantavirus IgM and/or IgG antibodies. The present study describes the expression of the N protein of a hantavirus detected in the blood sample of an HCPS patient. The entire S segment of the virus was amplified and found to be 1858 nucleotides long, with an open reading frame of 1287 nucleotides that encodes a protein of 429 amino acids. The nucleotide sequence described here showed a high identity with the N protein gene...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diagnosis; Hantavirus infection; Araraquara hantavirus; N protein.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700008

Revealing potential long non-coding RNA biomarkers in lung adenocarcinoma using long non-coding RNA-mediated competitive endogenous RNA network
Provedor de dados: 56
Autores: Zhu,T-G.; Xiao,X.; Wei,Q.; Yue,M.; Zhang,L-X.
In our study, we aimed to reveal potential long non-coding RNAs (lncRNA) biomarkers in lung adenocarcinoma (LAD) using lncRNA-mediated competing endogenous RNAs (ceRNAs) network (LMCN). Competing lncRNA-mRNA interactions were identified using the hypergeometric test. Co-expression analysis for the competing lncRNA-mRNA interactions was implemented, and relying on the weight value &gt;0.8, a highly competitive LMCN was further constructed. Degree distribution, betweenness and closeness for LMCN were carried out to analyze the network structure. Functional analyses of mRNAs in LMCN were carried out to further explore the biological functions of lncRNAs. Biclique algorithm was utilized to extract competing modules from the LMCN. Finally, we verified our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung adenocarcinoma; Competing endogenous RNA; Long non-coding RNA; Hub; Diagnosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900606

Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols
Provedor de dados: 56
Autores: de Moraes,F.M.; Espósito,D.L.A.; Klein,T.M.; da Fonseca,B.A.L.
Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika diagnosis is best achieved by real-time RT-PCR. However, some real-time RT-PCR show better performance than others. To reach the best possible Zika diagnosis, the analytic sensitivity of some probe-based real-time RT-PCR amplifying Zika virus RNA was evaluated in spiked and clinical samples. We evaluated primers and probes to detect Zika virus, which had been published before, and tested sensitivity using serum spiked and patient samples by real-time RT-PCR....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zika virus; Flavivirus; Real time RT-PCR; Diagnosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600618

Efficacy of the FEV1/FEV6 ratio compared to the FEV1/FVC ratio for the diagnosis of airway obstruction in subjects aged 40 years or over
Provedor de dados: 56
Autores: Rosa,F.W.; Perez-Padilla,R.; Camelier,A.; Nascimento,O.A.; Menezes,A.M.B.; Jardim,J.R.
The present cross-sectional, population-based study was designed to evaluate the performance of the FEV1/FEV6 ratio for the detection of airway-obstructed subjects compared to the FEV1/FVC &lt;0.70 fixed ratio test, as well as the lower limit of normality (LLN) for 1000 subjects ³40 years of age in the metropolitan area of São Paulo, SP, Brazil. After the exclusion of 37 (3.7%) spirometries, a total of 963 pre-bronchodilator (BD) and 918 post-BD curves were constructed. The majority of the post-BD curves (93.1%) were of very good quality and achieved grade A (762 curves) or B (93 curves). The FEV1/FEV6 and FEV1/FVC ratios were highly correlated (r² = 0.92, P &lt; 0.000). Two receiver operator characteristic curves were constructed in order to express the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forced expiratory volume in six seconds; Airway obstruction; Diagnosis; Forced vital capacity; Pulmonary function tests; Spirometry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200004

Use of the caffeine-halothane contracture test for the diagnosis of malignant hyperthermia in Brazil
Provedor de dados: 56
Autores: Sudo,R.T.; Cunha,L.B.P.; Carmo,P.L.; Matos,A.R.; Trachez,M.M.; Cardoso,L.A.M.; Aguiar,M.I.S.; Abreu,A.V.; Zapata-Sudo,G.
Malignant hyperthermia (MH) is a pharmacogenetic disease triggered by volatile anesthetics and succinylcholine. Deaths due to MH have been reported in Brazil. The first Malignant Hyperthermia Diagnostic and Research Center in Latin America was inaugurated in 1993 at the Federal University of Rio de Janeiro, Brazil. The center followed the diagnostic protocols of the North America MH Group, in which the contractures of biopsies from the vastus lateralis muscle are analyzed after exposure to caffeine and halothane (CHCT). CHCT was performed in individuals who survived, their relatives and those with signs/symptoms somewhat related to MH susceptibility (MHS). Here, we report data from 194 patients collected over 16 years. The Southeast (N = 110) and South (N...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant hyperthermia; Diagnosis; Skeletal muscle; Anesthesia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600005

Diagnostic and prognostic value of procalcitonin for early intracranial infection after craniotomy
Provedor de dados: 56
Autores: Yu,Y.; Li,H.J.
Intracranial infection is a common clinical complication after craniotomy. We aimed to explore the diagnostic and prognostic value of dynamic changing procalcitonin (PCT) in early intracranial infection after craniotomy. A prospective study was performed on 93 patients suspected of intracranial infection after craniotomy. Routine peripheral venous blood was collected on the day of admission, and C reactive protein (CRP) and PCT levels were measured. Cerebrospinal fluid (CSF) was collected for routine biochemical, PCT and culture assessment. Serum and CSF analysis continued on days 1, 2, 3, 5, 7, 9, and 11. The patients were divided into intracranial infection group and non-intracranial infection group; intracranial infection group was further divided into...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Intracranial infection; Procalcitonin; Cerebrospinal fluid procalcitonin; Craniotomy; Diagnosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500608

Cost-effective analysis of different algorithms for the diagnosis of hepatitis C virus infection
Provedor de dados: 56
Autores: Barreto,A.M.E.C.; Takei,K.; E.C.,Sabino; Bellesa,M.A.O.; Salles,N.A.; Barreto,C.C.; Nishiya,A.S.; Chamone,D.F.
We compared the cost-benefit of two algorithms, recently proposed by the Centers for Disease Control and Prevention, USA, with the conventional one, the most appropriate for the diagnosis of hepatitis C virus (HCV) infection in the Brazilian population. Serum samples were obtained from 517 ELISA-positive or -inconclusive blood donors who had returned to Fundação Pró-Sangue/Hemocentro de São Paulo to confirm previous results. Algorithm A was based on signal-to-cut-off (s/co) ratio of ELISA anti-HCV samples that show s/co ratio ³95% concordance with immunoblot (IB) positivity. For algorithm B, reflex nucleic acid amplification testing by PCR was required for ELISA-positive or -inconclusive samples and IB for PCR-negative samples. For algorithm C, all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; Immunoblot; ELISA; Polymerase chain reaction; Algorithm; Diagnosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200009

Evaluation of a 1-h 75-g oral glucose tolerance test in the diagnosis of gestational diabetes
Provedor de dados: 56
Autores: Campos,M.A.A.; Reichelt,A.A.J.; Façanha,C.; Forti,A.C.; Schmidt,M.I.
In order to evaluate the performance of a 1-h 75-g oral glucose tolerance test (OGTT) for the diagnosis of gestational diabetes mellitus (GDM), a cohort of 4998 women, 20 years or older, without previous diabetes being treated in prenatal care clinics in Brazil answered a questionnaire and performed a 75-g OGTT including fasting, 1-h and 2-h glucose measurements between their 24th and 28th gestational weeks. Pregnancy outcomes were transcribed from medical registries. GDM was defined according to WHO criteria (fasting: ≥126 mg/dL; 2-h value: ≥140 mg/dL) and macrosomia as a birth weight equal to or higher than 4000 g. Areas under the receiver operator characteristic curve (AUC) were compared and diagnostic properties of various cut-off points were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gestational diabetes; Diagnosis; Macrosomia; 1-h OGTT; Sensitivity; Specificity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800008

Chronic obstructive pulmonary disease is underdiagnosed and undertreated in São Paulo (Brazil): results of the PLATINO study
Provedor de dados: 56
Autores: Nascimento,O.A.; Camelier,A.; Rosa,F.W.; Menezes,A.M.B.; Pérez-Padilla,R.; Jardim,J.R.
Chronic obstructive pulmonary disease (COPD) is a common disease in adults over 40 years of age and has a great social and economic impact. It remains little recognized and undertreated even in developed countries. However, there are no data about its diagnosis and treatment in Brazil. The objectives of the present study were to evaluate the proportion of COPD patients who had never been diagnosed and to determine if the COPD patients who had been identified were receiving appropriate treatment. The Latin American Project for the Investigation of Obstructive Lung Disease (PLATINO) was a randomized epidemiological study of adults over 40 years living in five metropolitan areas, including São Paulo. The studied sample was randomly selected from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Diagnosis; Treatment; Vaccination; Smoking; Latin American Project for the Investigation of Obstructive Lung Disease.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700001

Monitoring human cytomegalovirus viral load in peripheral blood leukocytes of renal transplant recipients by a simple limiting dilution-PCR assay
Provedor de dados: 56
Autores: Caballero,O.L.; Costa,M.C.S.L.; Trevisan,A.; Oliveira,R.M.; Viotti,E.A.; Távora,E.R.F.; Vilaça,S.S.; Sabagga,E.; de-Paula,F.J.; Távora,P.F.; Brasileiro-Filho,G.; Villa,L.L.; Simpson,A.J.G.
To assess the clinical relevance of a semi-quantitative measurement of human cytomegalovirus (HCMV) DNA in renal transplant recipients within the typical clinical context of a developing country where virtually 100% of both receptors and donors are seropositive for this virus, we have undertaken HCMV DNA quantification using a simple, semi-quantitative, limiting dilution polymerase chain reaction (PCR). We evaluated this assay prospectively in 52 renal transplant patients from whom a total of 495 serial blood samples were collected. The samples scored HCMV positive by qualitative PCR had the levels of HCMV DNA determined by end-point dilution-PCR. All patients were HCMV DNA positive during the monitoring period and a diagnosis of symptomatic infection was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Human cytomegalovirus; Renal transplant; Diagnosis; Viral load.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200010

The reliability of the Brazilian version of the Composite International Diagnostic Interview (CIDI 2.1)
Provedor de dados: 56
Autores: Quintana,M.I.; Andreoli,S.B.; Jorge,M.R.; Gastal,F.L.; Miranda,C.T.
The objective of the present study was to determine the reliability of the Brazilian version of the Composite International Diagnostic Interview 2.1 (CIDI 2.1) in clinical psychiatry. The CIDI 2.1 was translated into Portuguese using WHO guidelines and reliability was studied using the inter-rater reliability method. The study sample consisted of 186 subjects from psychiatric hospitals and clinics, primary care centers and community services. The interviewers consisted of a group of 13 lay and three non-lay interviewers submitted to the CIDI training. The average interview time was 2 h and 30 min. General reliability ranged from kappa 0.50 to 1. For lifetime diagnoses the reliability ranged from kappa 0.77 (Bipolar Affective Disorder) to 1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Psychometrics scales; Reliability; Psychiatry; Composite International Diagnostic Interview (CIDI); Brazilian CIDI.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100020

Performance of a Brazilian population sample in the Boston Diagnostic Aphasia Examination: A pilot study
Provedor de dados: 56
Autores: Radanovic,M.; Mansur,L.L.
Brazilian researchers and health professionals often face the challenge of having to use tests developed in foreign languages and standardized for populations of other countries, especially in the fields of Neuropsychology and Neurolinguistics. This fact promotes a feeling that some scoring systems may be inadequate for our sociocultural reality. In the present study, we describe the performance of a Brazilian population sample submitted to a translated and adapted version of the Boston Diagnostic Aphasia Examination (BDAE). Sixty normal volunteers (21 men and 39 women), all Portuguese native speakers, ranging in age from 15 to 78 years (average 43.7) and with an educational level of 2 to 16 years (average 9.9), were tested using a translated and adapted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aphasia; Diagnosis; Portuguese.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300005

Extracellular vesicles: structure, function, and potential clinical uses in renal diseases
Provedor de dados: 56
Autores: Borges,F.T.; Reis,L.A.; Schor,N.
Interest in the role of extracellular vesicles in various diseases including cancer has been increasing. Extracellular vesicles include microvesicles, exosomes, apoptotic bodies, and argosomes, and are classified by size, content, synthesis, and function. Currently, the best characterized are exosomes and microvesicles. Exosomes are small vesicles (40-100 nm) involved in intercellular communication regardless of the distance between them. They are found in various biological fluids such as plasma, serum, and breast milk, and are formed from multivesicular bodies through the inward budding of the endosome membrane. Microvesicles are 100-1000 nm vesicles released from the cell by the outward budding of the plasma membrane. The therapeutic potential of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular vesicles; Cell biology; Kidney diseases; Diagnosis; Treatment.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000824

Use of conventional PCR and smear microscopy to diagnose pulmonary tuberculosis in the Amazonian rainforest area
Provedor de dados: 56
Autores: Carniel,F.; Dalla Costa,E.R.; Lima-Bello,G.; Martins,C.; Scherer,L.C.; Rossetti,M.L.
The diagnostic usefulness of Ziehl-Neelsen (ZN)-stained sputum smears combined with conventional polymerase chain reaction (ZN/PCR) to amplify IS6110 region DNA extracted from ZN slides was evaluated. The objective was to verify if this association could improve tuberculosis (TB) diagnosis in patients at remote sites. The study was carried out in 89 patients with culture-confirmed pulmonary TB as defined by the Brazilian Manual for TB Treatment. The participants were recruited in a reference unit for TB treatment in Rondônia, a state in the Amazonian area in northern Brazil. ZN, PCR, and culture performed in the sputum samples from these patients were analyzed in different combinations (i.e., ZN plus PCR and ZN plus culture). The prevalence rates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Diagnosis; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201016

Hereditary angioedema with C1 inhibitor (C1-INH) deficit: the strength of recognition (51 cases)
Provedor de dados: 56
Autores: Fragnan,N.T.M.L.; Tolentino,A.L.N.; Borba,G.B.; Oliveira,A.C.; Simões,J.A.; Palma,S.M.U.; Constantino-Silva,R.N.; Grumach,A.S.
Hereditary angioedema (HAE) is a rare autosomal dominant disease due to C1 esterase inhibitor deficiency (C1-INH). The disease is characterized by subcutaneous and submucosal edema in the absence of urticaria due to the accumulation of bradykinin. This descriptive study aimed to evaluate the clinical characteristics of patients with a confirmed diagnosis of HAE referred to our Outpatient Clinic between December 2009 and November 2017. Fifty-one patients (38 F, 13 M) with a mean age of 32 years (range: 7–70 y) were included. Family history of HAE was reported in 70% (36/51) of the cases; 33/46 patients became symptomatic by 18 years of age. The median time between onset of symptoms and diagnosis was 13 years (3 mo–50 y). The most frequent triggering factors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hereditary angioedema; C1 esterase inhibitor; Diagnosis; Treatment; Complement.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200607

Cytogenetic studies of Brazilian pediatric myelodysplastic syndrome cases: challenges and difficulties in a large and emerging country
Provedor de dados: 56
Autores: Velloso,E.D.R.P.; Chauffaille,M.L.; Peliçario,L.M.; Tanizawa,R.S.S.; Toledo,S.R.C.; Gaiolla,R.D.; Lopes,L.F.
Myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) are rare hematopoietic stem cell diseases affecting children. Cytogenetics plays an important role in the diagnosis of these diseases. We report here the experience of the Cytogenetic Subcommittee of the Brazilian Cooperative Group on Pediatric Myelodysplastic Syndromes (BCG-MDS-PED). We analyzed 168 cytogenetic studies performed in 23 different cytogenetic centers; 84 of these studies were performed in patients with confirmed MDS (primary MDS, secondary MDS, JMML, and acute myeloid leukemia/MDS+Down syndrome). Clonal abnormalities were found in 36.9% of the MDS cases and cytogenetic studies were important for the detection of constitutional diseases and for differential diagnosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Childhood myelodysplastic syndromes; Juvenile myelomonocytic leukemia; Cytogenetics; Karyotype; Diagnosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100085

Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
Provedor de dados: 58
Autores: Sales,Mariana L.; Fonseca Júnior,Antônio Augusto; Orzil,Lívia; Alencar,Andrea Padilha; Silva,Marcio Roberto; Issa,Marina Azevedo; Soares Filho,Paulo Martins; Lage,Andrey Pereira; Heinemann,Marcos Bryan.
Mycobacterium tuberculosis is the major cause of tuberculosis in humans. This bacillus gained prominence with the occurrence of HIV, presenting itself as an important opportunistic infection associated with acquired immunodeficiency syndrome (AIDS). The current study aimed to develop a real-time PCR using Eva Green technology for molecular identification of M. tuberculosis isolates. The primers were designed to Rv1510 gene. Ninety nine samples of M. tuberculosis and sixty samples of M. bovis were tested and no sample of the bovine bacillus was detected by the qPCR. Statistical tests showed no difference between the qPCR and biochemical tests used to identify the Mycobacterium tuberculosis. The correlation between tests was perfect with Kappa index of 1.0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Diagnosis; Real time PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400029

Improvement of an in-house ELISA for bovine paratuberculosis serology in Brazil
Provedor de dados: 58
Autores: Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R.
Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of bacilli at levels detectable by fecal culture is irregular and does not occur during the early stages of infection, which compromises the sensitivity of this methodology. The most common immunological tests to identify Map infection are complement fixation test (CFT), agar gel immunodiffusion (AGID) and enzyme-linked immunoassay (ELISA). In Brazil,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Cattle; ELISA; Serology; Diagnosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200004

Invasive Trichosporon cutaneum infection in an infant with wilms' tumor
Provedor de dados: 58
Autores: Carvalho,Ana Maria Rabelo de; Melo,Luciana Resende Bandeira de; Moraes,Vera Lúcia; Neves,Rejane Pereira.
Fungal infections are increasingly being reported in immuno-compromised patients. In this study we report a case of systemic Trichosporon cutaneum infection in an infant with Wilms' tumor. This is the first time that an invasive infection for T. cutaneum has been reported in a Wilms' tumor patient.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungal infections; Trichosporon cutaneum; An infant; Diagnosis; Wilms' tumor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100014

Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests
Provedor de dados: 58
Autores: Pourakbari,Babak; Ghazi,Mona; Mahmoudi,Shima; Mamishi,Setareh; Azhdarkosh,Hossein; Najafi,Mehri; Kazemi,Bahram; Salavati,Ali; Mirsalehian,Akbar.
Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. pylori, and correlate the diagnostic methods with PCR. Eighty nine patients (61 adults, 28 children) referred to the Firoozgar Hospital and Children Medical Center Hospital for diagnostic upper gastrointestinal endoscopy entered to the study and noninvasive tests such as immunoassay for serological antibodies against H. pylori and detection of its antigen in feces were measured. The biopsies were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Diagnosis; Invasive and noninvasive tests.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300020

Comparative study of Mycobacterium bovis primary isolation methods
Provedor de dados: 58
Autores: Issa,Marina de Azevedo; Soares Filho,Paulo Martins; Fonseca Júnior,Antônio Augusto; Hodon,Mikael Arrais; Santos,Lílian Cristian dos; Reis,Jenner Karlisson Pimenta dos; Cerqueira Leite,Rômulo.
Abstract For the definitive diagnosis of bovine tuberculosis, isolation of the etiologic agent is required. However, there is no consensus on the best methodology for isolation of Mycobacterium bovis in Brazil. This study evaluated the most used decontaminants and culture media in the country, in order to identify the best combination for the Brazilian samples. Three decontaminants - 2% sodium hydroxide (w/v), 0.75% hexadecylpiridinium chloride (w/v) and 5% sulphuric acid (v/v) and four culture media - 7H11 Middlebrook with additives and OADC supplement “A” (7H11 A), the same media with another supplement trademark (7H11 B), tuberculosis blood agar (B83) and Stonebrink's medium were compared. Regarding the isolation, there were no significant differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium bovis; Diagnosis; Middlebrook 7H11; Isolation; Sulphuric acid.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100139

Molecular typing of Mycobacterium bovis isolates: a review
Provedor de dados: 58
Autores: Ramos,Daniela Fernandes; Tavares,Lucas; Silva,Pedro Eduardo Almeida da; Dellagostin,Odir Antônio.
Mycobacterium bovis is the main causative agent of animal tuberculosis (TB) and it may cause TB in humans. Molecular typing of M. bovis isolates provides precise epidemiological data on issues of inter- or intra-herd transmission and wildlife reservoirs. Techniques used for typing M. bovis have evolved over the last 2 decades, and PCR-based methods such as spoligotyping and mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) have been extensively used. These techniques can provide epidemiological information about isolates of M. Bovis that may help control bovine TB by indicating possible links between diseased animals, detecting and sampling outbreaks, and even demonstrating cases of laboratory cross-contamination between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Bovine; Diagnosis; Genotyping; Mycobacterium bovis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200001

A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis
Provedor de dados: 58
Autores: Capizzani,Carolina Paulino da Costa; Caçador,Natália Candido; Marques,Elizabeth Andrade; Levy,Carlos Emílio; Tonani,Ludmilla; Torres,Lidia Alice Gomes Monteiro Marin; Darini,Ana Lúcia da Costa.
Abstract Identification of nonfermenting Gram-negative bacteria (NFGNB) of cystic fibrosis patients is hard and misidentification could affect clinical outcome. This study aimed to propose a scheme using polymerase chain reaction to identify NFGNB. This scheme leads to reliable identification within 3 days in an economically viable manner when compared to other methods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystic fibrosis; Nonfermenting Gram-negative bacteria; Identification scheme; Diagnosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200422

Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles
Provedor de dados: 58
Autores: Wang,Haihe; Zhao,Chunyan; Li,Fan.
Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific identification target to develop a novel hybridization signal amplification method (HSAM) for the rapid and direct detection of MTBC from clinical sputum specimens. This system consists of magnetic bead-linked capture probes for target isolation, dextranbased nanoparticles for amplifying the reporter molecule (biotinylated-FITC), and detection probes (2B-DNA) for binding the nanoparticles. Both the capture and detection probes were specific to the IS6110 target sequence. Our results determined that as few as 10 copies of the IS6110 sequence or 10 M. tuberculosis bacteria could be detected,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis complex (MTBC); Detection; Diagnosis; Rapid methods; Identification.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300016

Catalase activity of different Candida species after exposition to specific antiserum
Provedor de dados: 58
Autores: Miyasaka,Natália R.S.; Unterkircher,Carmelinda S.; Shimizu,Mario T.
Antisera were developed in rabbits after challenge with intracellular antigens of Candida albicans, C. tropicalis and C. parapsilosis. Microorganism catalase has been correlated with virulence, resistance to drugs and immunogenicity. The intracellular catalase is consistently present in strains of Candida and in this paper, the enzyme activity was analysed by PAGE after exposition to antisera. The catalases of C. albicans, C. parapsilosis and C. tropicalis were immunogenic and differed in their binding to specific antibodies raised in rabbits. Tests of cross-reactivity between different Candida species showed that when antiserum from C. albicans immunized rabbit was incubated with intracellular extracts of these three Candida species, the catalases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidiasis; Catalase; Candida; Antigen; Diagnosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100009

Laryngotracheitis: reproducibility of the disease and comparison of diagnostic methods
Provedor de dados: 58
Autores: Beltrão,Nilzane; Furian,Thales Quedi; Souza,Guilherme Fonseca de; Macagnan,Marisa; Fallavena,Luiz César Bello; Canal,Claudio Wageck.
Infectious laryngotracheitis virus (ILTV) cause mild to severe respiratory disease in chickens, the purpose of our study being to use Brazilian isolate of ILTV to reproduce ILTV disease in chickens by experimental infection and to compare three diagnostic methods (nested polymerase chain reaction (PCR), virus isolation, histopathology) for detection of ILTV. Forty-eight chickens intratracheally inoculated with ILTV and a further 48 with PBS, showing mild respiratory signs 48 hours post infection (PI) but no signs of infection after day 10 PI. Every 2 days PI, six birds were arbitrarily selected from the control and infected groups, sacrificed and the trachea collected. Both the nested PCR and virus isolation detected the virus from day 2 until day 12 PI....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious laryngotracheitis virus; Avian pathology; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500024

Isolation of leptospira Serovars Canicola and Copenhageni from cattle urine in the state of Paraná, Brazil
Provedor de dados: 58
Autores: Zacarias,Francielle Gibson da Silva; Vasconcellos,Silvio Arruda; Anzai,Eleine Kuroki; Giraldi,Nilson; Freitas,Julio Cesar de; Hartskeerl,Rudy.
In 2001, 698 urine samples were randomly collected from cattle at a slaughterhouse in the State of Paraná, Brazil. Direct examination using dark field microscopy was carried out immediately after collection. Five putative positive samples were cultured in modified EMJH medium, yielding two positive cultures (LO-14 and LO-10). Typing with monoclonal antibodies revealed that the two isolates were similar to Canicola (LO-14) and Copenhageni (LO-10). Microscopic agglutination test results show that Hardjo is the most common serovar in cattle in Brazil. Rats and dogs are the common maintenance hosts of serovars Copenhageni and Canicola. The excretion of highly pathogenic serovars such as Copenhageni and Canicola by cattle can represent an increasing risk for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Culture; Leptospirosis; Diagnosis; Cattle.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400028

Performance of a latex agglutination test in the diagnosis of acute gastroenteritis by rotavirus
Provedor de dados: 58
Autores: Ferreira,Thais Lourenço; Becho,Mariana Costa; Bernardo,Aline Rodrigues; Chaves,Thaís Castelo Branco; Ribeiro,Rosemary Suely; Lima,Jurandir Sampaio de; Fialho,Alexandre Madi; Leite,José Paulo Gagliardi; Mazur,Carlos; Danelli,Maria das Graças Miranda.
The aim of this work was to determine the performance of latex agglutination test (LAT) for evaluating children acute gastroenteritis by rotavirus. The LAT showed good sensitivity, as well as specificity and predictive positive value and due to its simplicity and speed, it has been suitable for rotavirus diagnosis in hospital laboratories.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rotavirus; Diagnosis; Latex agglutination test; Poliacrylamide gel electrophoresis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400035

Potential application of new diagnostic methods for controlling bovine Tuberculosis in Brazil
Provedor de dados: 58
Autores: Medeiros,Luciana dos Santos; Marassi,Carla Dray; Figueiredo,Eduardo Eustáquio S.; Lilenbaum,Walter.
Bovine tuberculosis, a chronic infection in cattle caused by Mycobacterium bovis, remains an economic and public health problem for several countries. Due to its economic impact on international trade, contagious nature, and implications for human health, global programs to eradicate the disease were implemented worldwide. Those programs are based on slaughtering PPD-reactive animals. Despite the National Programs in Brazil, complete eradication has not been achieved, and the disease remains, albeit at a lower prevalence. The central purpose of this review is to address diagnostic tests for tuberculosis. Considering the course of the infection in cattle, at least two tests, ideally complementary to one another, may be necessary for an adequate diagnosis:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine Tuberculosis; Mycobacterium bovis; Diagnosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300002

Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
Provedor de dados: 58
Autores: Spositto,F.L.E.; Campanerut,P.A.Z.; Ghiraldi,L.D.; Leite,C.Q.F.; Hirata,M.H.; Hirata,R.D.C.; Siqueira,V.L.D.; Cardoso,R. Fressatti.
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C &gt; G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine tuberculosis; Tuberculosis; PCR-multiplex; Diagnosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300012

Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)
Provedor de dados: 58
Autores: Freschi,Carla Roberta; Carvalho,Luiz Fernando de Oliveira e Silva; Oliveira,Celso José Bruno de.
The aim of this study was to compare different DNA-extraction methods and selective enrichment broths for their effectiveness to detect Salmonella Typhimurium in artificially inoculated swine feces samples (100 CFU/g) by polymerase chain reaction. After enrichment in Rappaport-Vassiliadis, selenite cystine or Müller-Kauffmann tetrathionate, aliquots were used for DNA extraction by three different methods: boiling-centrifugation, phenol-chloroform and salting-out. Aliquots of extracted DNA were then used as template in PCR. The selective enrichment broths had no effect on the efficiency of PCR when boiling-centrifugation and salting-out were used. On the other hand, phenol-chloroform was superior (P&lt;0.05) when combined to Rappaport-Vassiliadis....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; DNA; Feces; PCR; Salmonella Typhimurium.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400011

Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil
Provedor de dados: 58
Autores: Albuquerque,Pedro Paulo Feitosa de; Santos,André de Souza; Souza Neto,Orestes Luiz de; Kim,Pomy de Cássia Peixoto; Cavalcanti,Erika Fernanda Torres Samico Fernandes; Oliveira,Júnior Mário Baltazar de; Mota,Rinaldo Aparecido; Pinheiro Júnior,José Wilton.
Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular biology; Diagnosis; Paratuberculosis; Milk.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100113

Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
Provedor de dados: 58
Autores: Silva,Karla Patrícia Chaves da; Takaki,Galba Maria de Campos; Silva,Leonildo Bento Galiza da; Saukas,Tomoe Noda; Santos,André Souza; Mota,Rinaldo Aparecido.
To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders. These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no clinical signs and were serology negative (control group). PPD-mallein from Burkholderia mallei was obtained by precipitation with trichlo-roa-cetic acid and ammonium sulfate. The animals were inoculated according to the criteria established by Department of Agriculture, Livestock and Supply (MAPA) for the diagnosis of glanders. After 48 h of application of PPD-mallein, there was swelling in the area of application, presence of ocular secretion and tears in sick animals. The control group showed no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mallein test; Equus asinus; Diagnosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100027

Seroprevalence of antibodies to Chlamydophila abortus in ovine in the State of Alagoas, Brazil
Provedor de dados: 58
Autores: Pinheiro Junior,José Wilton; Mota,Rinaldo Aparecido; Piatti,Rosa Maria; Oliveira,Andréa Alice da Fonseca; Silva,Aline Melo da; Abreu,Sílvio Romero de Oliveira; Anderlini,Giulliano Aires; Valença,Rômulo Menna Barreto.
The goal of this study was to perform a seroepidemiological investigation and to identify risk factors associated with infection of Chlamydophila abortus of sheep herds in the Brazilian state of Alagoas. The study was conducted with samples of 274 ewes with ages equal to or higher than 24 months in 25 herds and in 23 towns located in three regions of the state (Sertão, Agreste and Eastern Alagoas). Anti-C. abortus antibodies were detected using the microcomplement fixation test. The risk factors, were determined based on questionnaires consisting of objective questions, about the farmer and general characteristics of the herd like size, sanitary situation and reproductive management. Among 274 sera samples analyzed for C. abortus, 59 (21.5%) were positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Chlamydiosis; Serum epidemiology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200015

Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems
Provedor de dados: 58
Autores: Yamaguti,M.; Muller,E.E.; Piffer,A.I.; Kich,J.D.; Klein,C.S.; Kuchiishi,S.S.
Since Mycoplasma hyopneumoniae isolation in appropriate media is a difficult task and impractical for daily routine diagnostics, Nested-PCR (N-PCR) techniques are currently used to improve the direct diagnostic sensitivity of Swine Enzootic Pneumonia. In a first experiment, this paper describes a N-PCR technique optimization based on three variables: different sampling sites, sample transport media, and DNA extraction methods, using eight pigs. Based on the optimization results, a second experiment was conducted for testing validity using 40 animals. In conclusion, the obtained results of the N-PCR optimization and validation allow us to recommend this test as a routine monitoring diagnostic method for Mycoplasma hyopneumoniae infection in swine herds.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine Enzootic Pneumonia; Mycoplasma hyopneumoniae; Diagnosis; Nested- PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300011

Brucella spp. isolation from dogs from commercial breeding kennels in São Paulo state, Brazil
Provedor de dados: 58
Autores: Keid,Lara B.; Soares,Rodrigo M.; Morais,Zenaide M.; Richtzenhain,Leonardo J.; Vasconcellos,Sílvio A.
Dogs from 12 commercial breeding kennels were submitted to clinical investigation and laboratorial tests for diagnosis of Brucella spp. infection. The sampling was carried out between April 2000 and February 2002 and the laboratorial tests employed were agar gel immunediffusion test (AGID) and blood culture. From 171 dogs examinated, 39 (22.8%) showed at least one clinical sign compatible with brucellosis, 58 (33.91%) were AGID positive and 24 (14.03%) were positive by blood culture. Gram negative bacterial cells with a biochemical pattern compatible with that of bacteria belonging to genus Brucella were isolated from blood specimens of 24 animals. According to Kappa index and McNemar test, the association between AGID and blood culture (k=0.360 with 95%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Brucellosis; Brucella canis; Diagnosis; Isolation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100027

Identification of bacterial agents of enteric diseases by multiplex PCR in growing-finishing pigs
Provedor de dados: 58
Autores: Baccaro,Maria Regina; Moreno,Andrea Micke; Shinya,Luciane Tieko; Dotto,Daniela Sabatini.
In Brazil, the most common bacterial enteric diseases affecting growing and finishing pigs are porcine proliferative enteritis, porcine intestinal spirochetosis, swine dysentery, and salmonellosis. The diagnosis of these diseases by routine culture techniques is expensive, difficult, time-consuming, and even impossible, in cases of porcine proliferative enteritis. The detection of pathogens by polymerase chain reaction is a highly sensitive and specific method that can be an useful tool in veterinary diagnosis. Two multiplex PCR (M-PCR) assays were tested for simultaneous detection and identification of bacterial agents associated with porcine proliferative enteritis, porcine intestinal spirochetosis, swine dysentery, and salmonellosis in diarrheic fecal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteritis; PCR; Pig; Bacteria; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300008

Detection of Mycoplasma pulmonis in laboratory rats
Provedor de dados: 58
Autores: Barreto,Maria Lucia; Nascimento,Elmiro Rosendo do; Campos,Carlos Augusto de Martino; Nascimento,Maria da Graça Fichel do; Lignon,Gilberto Brasil; Lira,Marie Luce Flores; Silva,Ricardo G.
This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma pulmonis; Diagnosis; Isolation; Serology; Rats.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300015

Development of an indirect ELISA based on recombinant capsid protein to detect antibodies to bovine leukemia virus
Provedor de dados: 58
Autores: Andreolla,Ana Paula; Erpen,Luana Marina Scheer; Frandoloso,Rafael; Kreutz,Luiz Carlos.
Abstract Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp ® plate coated with 50 ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450 nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine leukemia virus; Retrovirus; Diagnosis; ELISA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500068

Circulating cell-free DNA as a biomarker in the diagnosis and prognosis of colorectal cancer
Provedor de dados: 60
Autores: Oliveira,Isadora Bernardo David de; Hirata,Rosario Dominguez Crespo.
Abstract Colorectal cancer (CRC) is a disease without evident clinical symptoms in early stages, leading to late diagnosis and disease management. Current diagnostic and prognostic tools require invasive procedures and circulating molecular biomarkers fail to have optimal sensitivity and specificity. Circulating biomarkers with high clinical performance may be valuable for early diagnosis and prognosis of CRC. The purpose of this review was to investigate the application of circulating cell-free DNA (ccfDNA) in CRC diagnosis and prognosis and the analytical methods used in blood samples in articles published between 2005 and 2016. Based on specific inclusion and exclusion criteria, 26 articles were selected. Most studies used ccfDNA quantification as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal Neoplasms/diagnosis; Colorectal cancer; Circulating cell-free DNA; CcfDNA; Prognosis; Diagnosis; Biomarkers; Neoplastic Cells/circulating.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100406

Perennial grasses traits as functional markers of grazing intensity in basaltic grasslands of Uruguay
Provedor de dados: 117
Autores: Jaurena,Martin; Lezama,Felipe; Cruz,Pablo.
Natural grasslands in the basaltic region of Uruguay are threatened by an increase in stocking rates and changes in land use. To assess the effect of grazing intensification, plant functional types are proposed as simple tools to aid the monitoring and management of vegetation. In the present study we evaluated the effect of stocking rate increase at community level taking into account plant traits of 23 dominant perennial grass species. In order to identify plant functional types, we determined the grazing response in an experiment with two wethers stocking rates (0.78 and 1.56 livestock units ha-1) quantifying species cover and traits values. Leaf dry matter content (LDMC) and specific leaf area (SLA) were the traits that best described the perennial...
Tipo: Journal article
Palavras-chave: Natural grasslands; Diagnosis; Management; Stocking rate.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000400013

SEROLOGICAL DIAGNOSIS OF EQUINE INFECTIOUS ANEMIA VÍRUS INFECTION IN THE CENTRAL REGION OF THE RIO GRANDE DO SUL STATE
Provedor de dados: 65
Autores: Rebelatto,Marlon Cezar; Oliveira,Clóvis de; Weiblen,Rudi; Silva,Saul Fontoura da; Oliveira,Luiz Sérgio Segala de.
This paper consists of a brief review on equine Infectious anemia added of the results of serological diagnosis of this infection performed at the Federal University of Santa Maria, RS, Brazil, between 1979 to 1990. A total of 7,035 serum samples were tested by the agar gel immunodiffusion test (Coggins test), to which 40 (0,57%) reacted positively. This percentage of positivity is lower than in other regions of Brazil, probably due to the lack of predisposing factors for equine infectious anemia virus spread, such as, a low density of blood-sucking insects in the South of Brazil and the seldom use of massive therapies and vaccinations in the region.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine infectious anemia; Diagnosis; Immunodiffusion.
Ano: 1992
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781992000200012

Molecular detection of protozoa of the Sarcocystidae family in sheep from the State of Rio Grande do Sul, Brazil
Provedor de dados: 65
Autores: Portella,Luiza Pires; Cadore,Gustavo Cauduro; Sangioni,Luis Antonio; Alves,Marta Elena Machado; Chemeris,Raiza; Brum,Larissa Picada; Vogel,Fernanda Silveira Flores.
ABSTRACT: Infections caused by protozoa belonging to the Sarcocystidae family have worldwide distribution and are common in ruminants, leading to considerable economic losses. This study evaluates Sarcocystis spp.,  Toxoplasma gondii and Neospora caninum infections in sheep from Southwest region of Rio Grande do Sul, Brazil. Myocardium samples of 80 sheep raised on extensive system were collected. Tissue cysts were detected by direct examination and presence of infective agents was confirmed by PCR. Macroscopic evaluation did not reveal changes, but direct microscopic examination showed cysts in 76.2% (61/80, 95% CI: 66.9 - 85.9) samples, and all cysts were morphologically similar to those caused by  Sarcocystis tenella or Sarcocystis arieticanis. PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sarcocystis spp.; Toxoplasma gondii; Neospora caninum; PCR; Diagnosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901613

Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions
Provedor de dados: 65
Autores: Silva,Claudênia Ferreira da; Uesugi,Carlos Hidemi; Blum,Luiz Eduardo Bassay; Marques,Abi Soares dos Anjos; Ferreira,Marisa Álvares da Silva Velloso.
ABSTRACT:  Erwinia psidii causes bacterial blight of guava ( Psidium guajava ), an important disease of this crop in Brazil. The pathogen affects branches and twigs of guava trees, reducing yield significantly. Bacterial dissemination often occurs through contaminated but asymptomatic propagating plant material. The objectives of this research were to evaluate the use of BIO-PCR and conventional PCR to detect E. psidii in inoculated guava plants grown in a greenhouse and in symptomatic and asymptomatic trees from guava orchards. Erwinia psidii strain IBSBF 1576 was inoculated (107CFU mL-1) into young guava shoots and plant tissue was analysed at 0, 5, 10, and 15 days after inoculation. Symptoms were observed after 5 days and all inoculated shoots were PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BIO-PCR; Diagnosis; Guava bacterial blight; PCR; Psidium guajava.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901528

Knowledge management for the sustainable development of the semi-arid region in Northeastern Brazil
Provedor de dados: 65
Autores: Brito,Lydia Maria Pinto; Silva,Naeldson Expedito Alves da; Castro,Ahiram Brunni Cartaxo de; Nodari,Cristine Hermann; Silva,Arthur William Pereira da.
ABSTRACT:  The objective of this study was to analyze the knowledge management perception in managers of a public university in the Brazilian Northeastern Semiarid Region, especially their tactical and strategic processes to produce knowledge about this region. The analysis was conducted according to the perspective of administrators at this university with a view to the sustainable development of the region. This was a qualitative descriptive study. All data were collected through structured interviews, using a questionnaire based on the Knowledge Management Diagnosis as a structuring model. Results showed that Knowledge Management is still a maturing practice. However, knowledge management is making institutional changes feasible and generating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Knowledge management; Public administration; University; Semi-arid region; Northeastern Brazil; Sustainable development.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000400931

A review on the diagnosis infection in cattle of Schistosoma bovis: current status and future prospects
Provedor de dados: 65
Autores: Aradaib,Imadeldin Elamin; Ahdelmageed,Elabbas Mohamed; Hassan,Sanaa Ali; Riemann,Hans Peter.
Bovine schistosomiasis, caused by Schistosoma bovis, is a serious veterinary problem in many parts of the worid. The current methods used for the diagnosis of the disease include clinical signs, pathological lesions, parasitological and serological techniques. As clinical signs and parasitological lesions caused by S. bovis are indistinguishable from those induced by other trematode parasites, confirmation of diagnosis by these methods is unreliable. Parasitological techniques used to demonstrate eggs of the parasite in fecal or tissue samples represent the most accurate method for detection of an active S. bovis infection. The tissue of choice for detection of S. bovis infection is the liver because of the visible macroscopic lesion that can be seen in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma bovis; Bovine schistosomiasis; Diagnosis; Cattle.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781995000300029

Hemophagocytic histiocytic sarcoma in feline
Provedor de dados: 65
Autores: Teixeira,Luciele Varaschini; Martins,Danieli Brolo; França,Raqueli Teresinha; Amaral,Anne Santos do; Mazzanti,Cínthia Melazzo; Lopes,Sonia Terezinha dos Anjos.
Cytological analysis of the pleural cavity becomes extremely important for the diagnosis of tumors that have no visible or palpable mass. The aim of this study is to report a case of hemophagocytic histiocytic sarcoma in a cat, a rare neoplasm, emphasizing the importance of laboratory tests, especially the analysis of effusions. This report discloses the usefulness of cytology in effusions directing the clinical veterinarian for the correct diagnosis and subsequent treatment.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Neoplasms; Cat; Diagnosis; Effusion analysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000400016

Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe
Provedor de dados: 65
Autores: Fajardo,Thor Vinícius Martins; Nickel,Osmar.
The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was sufficiently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specificity was confirmed by reaction with homologous viral cDNA, individually cloned for each virus.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Diagnosis; Malus; Pyrus; Polyprobe; ASGV; ACLSV; ASPV; ApMV.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001001711

Nested-PCR multiplex test with increased sensitivity for detection of allogeneic cells transplanted from male to female mice
Provedor de dados: 65
Autores: Cezar,Alfredo Skrebsky; Glanzner,Werner Giehl; Gutierrez,Karina; Anezi-Junior,Paulo Afonso; Nilles,Carlise Reichert; Comim,Fábio Vasconcellos; Vogel,Fernanda Silveira Flores; Gonçalves,Paulo Bayard Dias.
Cell therapy has shown encouraging perspectives for human and veterinary medicine. Experimentally, genetic manipulation allows to mark and locate allogeneic cells. However, this makes their genotype/phenotype different from non-marked cells used clinically. Alternatively, the presence of the Y-chromosome enables male donor cells detection in female organisms. However, the concentration of engrafted cells may be minimal in tissues, due to systemic distribution. In this study, a nested-PCR multiplex test was developed, aiming to increase the sensitivity of the presence/absence diagnosis of male mice adipose-derived (ADSC-Y) and bone marrow mononuclear (BMNC-Y) cells in samples of blood and lungs from females, after endovenous transplantation. Four females...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellular therapy; Transplantation; Allogeneic cells; Diagnosis; Y-chromosome.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500905

Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil
Provedor de dados: 65
Autores: Fajardo,Thor Vinícius Martins; Nascimento,Monique Bezerra; Eiras,Marcelo; Nickel,Osmar; Pio-Ribeiro,Gilvan.
ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Diagnosis; Sequencing; Ilarvirus; PNRSV; ApMV; ArMV.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202197

Can vaccines against canine visceral leishmaniasis interfere with the serological diagnostics recommended by the Brazilian Ministry of Health?
Provedor de dados: 65
Autores: Campos,Monique Paiva de; Luca,Paula Mello De; Renzetti,Alinne Rangel dos Santos; Souza,Sara Maria Marques de; Mendes Júnior,Artur Augusto Velho; Barros,Renata Simões; Figueiredo,Fabiano Borges.
ABSTRACT: The objective of the current research was to assess seroconversion in dogs immunized with Leishmune® and Leish Tec® vaccines using rapid chromatographic immunoassay DPP® (Dual Path Platform) (DPP CVL) and enzyme immunoassay (EIE) up to one year after the vaccination protocol. The study sample comprised 28 dogs divided into two groups, each group immunized with an anti-CVL vaccine and clinically monitored for one year through clinical evaluation and laboratory tests. 22 (78.5%) dog were monitored. During the evaluation time (T1-30 days, T2-6 months, and T3-1 year after vaccination) the results for all dogs were negative for CVL, except for one animal vaccinated with Leish tec® that seroconverted in the DPP CVL test at T2. Subsequent examinations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine visceral leishmaniasis; Vaccine; Diagnosis; Seroconversion.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000400503

Parasitological analysis of synovial fluid from dogs naturally infected with Leishmania sp.
Provedor de dados: 65
Autores: Silva,Mayara Nobrega Gomes da; Pereira,Deyverson Thiago Prates; Mistieri,Maria Ligia de Arruda; Lubeck,Irina.
The aim of this study was to verify the feasibility of synovial fluid (SF) analysis for the parasitological diagnosis of leishmaniasis in naturally infected dogs. Aseptic arthrocentesis of the tarsal and carpal joints of 40 infected dogs (158 Leishmania sp samples) was conducted after euthanasia. The SF smears were evaluated under optic microscopy and amastigotes were detected in the SF of 35 dogs (87.5%). Of the 93 positive samples, 53% were obtained from the tarsal joints, and 47% were obtained from the carpal joints. Amastigote forms were confirmed in one (17%), two (26%), three (31%) or all four investigated joints (26%). This research highlights the feasibility and high sensitivity of SF evaluation for the parasitological diagnosis of leishmaniasis in...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Leishmaniasis; Canine; Diagnosis; Joint.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000701236

Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples
Provedor de dados: 65
Autores: Makino,Herica; Sousa,Valéria Régia Franco; Fujimori,Mahyumi; Rodrigues,Juliana Yuki; Dias,Alvaro Felipe Lima Ruy; Dutra,Valéria; Nakazato,Luciano; Almeida,Arleana do Bom Parto Ferreira de.
ABSTRACT: The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine ehrlichiosis; Diagnosis; Nested PCR; Biological samples.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200310

Felis catus gammaherpesvirus 1 (FcaGHV1) and coinfections with feline viral pathogens in domestic cats in Brazil
Provedor de dados: 65
Autores: Kurissio,Jacqueline Kazue; Rodrigues,Marianna Vaz; Taniwaki,Sueli Akemi; Zanutto,Marcelo de Souza; Filoni,Claudia; Galdino,Maicon Vinícius; Araújo Júnior,João Pessoa.
ABSTRACT: Felis catus gammaherpesvirus 1 (FcaGHV1) may causes an asymptomatic infection that result in an efficient transmission and subsequently dissemination of the virus in feline population. This study used molecular detection by qPCR (quantitative PCR) based on DNA polymerase gene fragment amplification to evaluate the occurrence of FcaGHV1 and its correlation with other feline viral pathogens, such as Carnivore protoparvovirus 1 (CPPV-1), Felid alphaherpesvirus 1 (FeHV-1), and feline retroviruses such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). Of the 182 blood samples evaluated 23.6% (43/182) were positives for FcaGHV1. Approximately 37.9% (33/87) of the samples that tested positive for retrovirus were also were positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gammaherpesvirus; Herpesvirus; Feline pathogen; Infectious diseases; Diagnosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300451

Detection and partial molecular characterization of Grapevine fleck virus, Grapevine virus D, Grapevine leafroll-associated virus -5 and -6 infecting grapevines in Brazil
Provedor de dados: 65
Autores: Fajardo,Thor Vinícius Martins; Eiras,Marcelo; Nickel,Osmar; Dubiela,Carla Rosa; Souto,Eliezer Rodrigues de.
Grapevine fleck, rugose wood and leafroll are three grapevine viral diseases whose causal agents (or associated viruses) respectively are Grapevine fleck virus (GFkV), Grapevine virus D (GVD) and Grapevine leafroll-associated virus 5 and 6 (GLRaV-5 and -6). The objective of this work was to perform a partial molecular characterization of local isolates of these four viral species that infect grapevines. The nucleotide and deduced amino acid sequences of complete genes of the coat protein (CP) (of GFkV), the CP and the RNA binding protein (of GVD), the CP and the partial hHSP70 gene (of GLRaV-5) and the partial hHSP70 gene (of GLRaV-6) were aligned and compared in silico with other isolates. These data extend the available information about Brazilian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Sequencing; Maculavirus; Vitivirus; Ampelovirus; Vitis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012001200004

Laparoscopy hepatic biopsy through cauterization
Provedor de dados: 65
Autores: Fantinatti,Alexandra Pinheiro; Daleck,Carlos Roberto; Nunes,Newton; Alessi,Antonio Carlos; Costa Neto,João Moreira da; Vicenti,Felipe Antonio Mendes; Duque,Juan Carlos; Santos,Paulo Sérgio Patto dos.
Hepatic biopsy was realized through laparoscopy with simultaneous cauterization in the present study, whose principal aim was to evaluate the efficacy of the applied method and to study its effects in the liver of healthy dogs. Furthermore, we tried to verify the main hematological and chemistry profile alterations related to the hepatic function, and to investigate the viability of the fragments collected by histopathology. To attain this objective, 21 clinically healthy dogs, weighing between 10 and 15kg were submitted to hepatic biopsy with forceps connected to the cautery. Cautery was performed by applying radiofrequency energy at 45 watts. Forty-two hepatic biopsies through laparoscopy were conducted in the animals. At group I one hepatic fragment per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrocautery; Dog; Surgery; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000400019

Detection of Ehrlichia canis in bone marrow aspirates of experimentally infected dogs
Provedor de dados: 65
Autores: Moreira,Simone Magela; Machado,Rosângela; Passos,Lygia Friche.
The present work describes the detection of infected cells in the bone marrow aspirates of dogs experimentally infected with a Brazilian isolate of Ehrlichia canis. Dogs were monitored twice a day by clinical evaluation and peripheral blood smear examination. Every three days, blood samples were collected for cell counts. Weekly, aspirates from the bone marrow were examined and serum samples were tested by IFAT. The clinical signs observed were fever, pallid membranes, lymphadenopathy, serous nasal secretions, and pronounced weight loss. Hematological alterations included normocytic normochromic anemia, decrease of neutrophils and lymphocytes, and thrombocytopenia. Few E. canis infected cells were seen in blood smears. However, stages of E. canis were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ehrlichia canis; Diagnosis; Bone marrow puncture; Dogs.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000400038