Consulta: subjectFacets:"Diagnostic"
Registros recuperados: 51
Data/hora: 29/05/2024 02:16:21

Diagnóstico da piscicultura na Bacia do Alto Taquari - MS.
Provedor de dados: 14
Autores: ROTTA, M. A.
O objetivo deste trabalho é a caracterização da situação da piscicultura na bacia do alto Taquari (BAT) a fim de se propor melhorias no seu sistema, viabilizando-o como meio alternativo de renda para os produtores da região. Este estudo foi realizado no mês de abril de 2003 através de levantamento com a aplicação de um questionário aos piscicultores da BAT. Foram obtidas informações para caracterizar os produtores, a propriedade e a produção, além de outros aspectos relevantes à atividade. Dos 24 produtores localizados na BAT, 12 (50%) se encontram em atividade, 10 estão inativos (42%) e em dois deles (8%) não foi possível obter informações. Na BAT a atividade de piscicultura vem sendo realizada principalmente em grandes propriedades, com área média de...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Caracterização; Bacia do Alto Taquari; Mato Grosso do Sul; Brasil; Diagnostic; Production; Scrutiny; Characterization; Taquari river; Wetland.; Diagnostico; Levantamento; Peixe; Produção.; Piscicultura; Brazil; Pantanal; Fish culture.; Fish.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/786918

Ferida da moda: epidemiologia, diagnóstico, tratamento e experiência com eqüinos infectados no Pantanal.
Provedor de dados: 14
Autores: SANTURIO, J. M.; CATTO, J. B.; COMASTRI FILHO, J. A.; LEAL, A. T.; LEAL, A. B. M.
A pitiose é uma doença granulomatosa que atinge eqüinos, caninos, bovinos, felinos e humanos e ocorre em áreas tropicais, subtropicais ou temperadas (Meireles et al., 1993; Mendoza et al., 1996), causada pelo omiceto Pythium insidiosum (De Cock et al., 1987). A espécie eqüina é a mais atingida, principalmente nas formas cutânea e subcutânea, seguida dos caninos (Mendoza et al., 1996). A enfermidade em eqüinos caracteriza-se pela formação de granulomas eosinofílicos, com a presença de massas necróticas chamadas de kunkers (Mendoza e Alfaro, 1986; Meireles et al., 1993). Em humanos, a doença é mais comum no sudeste asiático, caracterizando-se por formas sistêmicas com arterites e também formas oftálmicas e subcutâneas, sendo as duas últimas associadas a a e...
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Ferida da moda; Pitiose; Diagnostic; Equine.; Diagnostico; Eqüino; Epidemiologia; Tratamento.; Pantanal; Pythium insidiosum; Epidemiology.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/811096

A campilobacteriose e tricomonose são doenças reemergentes?
Provedor de dados: 14
Autores: PELLEGRIN, A. O.
Epidemiologia; Prevalência e incidência no Brasil; Fatores e risco; Impactos econômicos; Diagnóstico; Controle.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Controle; Bovine; Disease; Campylobacter fetus venerealis; Tritichomonas foetus; Diagnostic; Control.; Bovino; Campilobacteriose; Doença; Diagnostico; Epidemiologia; Pecuária; Tricomonose.; Epidemiology.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/810729

Possíveis causas de aborto em caprinos: diagnóstico, tratamento, profilaxia.
Provedor de dados: 14
Autores: SILVA, M. U. D. e; SILVA, E. D. F. da.
Fornece conceitos de base alertando técnicos e criadores sobre um problema já existente.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Disease prophylaxis; Abortion; Therapy.; Diagnostic; Caprino; Aborto; Diagnóstico; Veterinária; Profilaxia; Reprodução animal; Goats; Pregnancy complications; Animal diseases.
Ano: 1983
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/514903

Enregistrement d'images vidéo géoréférencées par caméra remorquée dans les lagunes de Méditerranée. Expérimentations pilotes dans les étangs de Leucate (Juin 2004), de Thau et d'Ingril (avril 2005).
Provedor de dados: 5
Autores: Oheix, Jocelyne.
Dans le cadre du Réseau de Suivi Lagunaire, des images vidéo géoréférencées par caméra tractée ont été réalisées dans trois lagunes de Méditerranée : Thau, Leucate et Ingril avec la collaboration de l'équipe d'Ifremer/Toulon qui assure le développement technique de l'outil d'acquisition d'images dans le cadre du projet « Prisme ». L'objectif est de déterminer la faisabilité technique en lagune puis d'optimiser l'exploitation des données. Ce rapport présente les résultats de la mise en œuvre de la caméra tractée, du rejeu des images et de la représentation cartographique des données sous SIG. Ces expérimentations ont permis de définir une typologie d'utilisation de la vidéo tractée en lagune: - Recouvrement végétal, - Cartographie d'herbier, - Grand type de...
Tipo: Text
Palavras-chave: Lagunes littorales; Méditerranée; Vidéo sous-marine remorquée; Diagnostic; Macrophytes; Coastallagoon; Mediterranean; Towed submarine video; Diagnostic; Macrophytes.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00113/22400/20092.pdf

Contribution à la validation et à l'optimisation de la technique de PCR pour la détection de Bonamia ostreae chez l'huître plate : Ostrea edulis
Provedor de dados: 5
Autores: Touzeau, Damien.
L'ostréiculture représente une part économique régionale et française importante avec une production d'environ 117 106 Tonnes de coquillages par an (FAO, 2003). Cependant le développement de cette activité peut être limité par des mortalités, parfois massives, dues à des maladies infectieuses. L'huître plate Ostrea edulis a subi deux maladies majeures : la Marteiliose due à Marteilia refringens et la Bonamiose due à Bonamia ostreae. Ces maladies à déclaration obligatoires ont fait chuter la production de 20 000 tonnes dans les années 70 à 2 098 Tonnes aujourd'hui. Suite à ces mortalités, différents programmes de recherche ont été lancés par Ifremer pour tenter de préserver voire de relancer la culture de l'huître plate. Un de ces programmes concerne la...
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Parasite; Bonamia ostreae; Technique PCR; ARN 18 S; Diagnostic.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00034/14478/11777.pdf

Diagnostic quantitatif de Perkinsus atlanticus (Apicomplexa, Perkinsea) chez les palourdes Ruditapes decussatus et Ruditapes philippinarum
Provedor de dados: 5
Autores: Almeida, Manuela.
Le protozoaire parasite Perkinsus atlanticus (Azevedo, 1989) est une des causes des chutes de production de palourdes Ruditapes decussatus et R. philippinarum cultivées au Portugal, en Espagne et en Italie. Des modèles d'infection de Perkinsus ont été réalisés, par reproduction expérimentale de la maladie, pour étudier le rôle de la densité des palourdes, de la température et de la salinité sur le déroulement de la Perkinsiose. L'objectif de ce travail a été de développer une technique de diagnostic quantitative pour optimiser l'évaluation de l'intensité d'infection et détecter de faibles infections des palourdes par P. atlanticus. Le principe de cette technique consiste à cultiver tous les tissus de la palourde en milieu thioglycolate, suivi d'une lyse à...
Tipo: Text
Palavras-chave: Pathologie; Protozoaire; Parasite; Perkinsus atlanticus; Bivalvia; Ruditapes decussatus; Ruditapes philippinarum; Diagnostic.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00033/14416/11710.pdf

Amélioration du protocole de PCR quantitative pour la détection de l'OsHV1 chez l'huître creuse, Crassostrea gigas, dans le cadre du projet TRIPLOFIMER et suivi d'individus lors du phénomène de mortalité estivale dans le cadre du projet AQUAFIRST
Provedor de dados: 5
Autores: Riou, Antoine.
Depuis 1991, des épisodes de mortalités massives touchent les élevages ostréicoles français, notamment pour l'espèce Crassostrea gigas. Un des facteurs responsables de ces événements est le virus de l'Herpès de l'huître : Oyster Herpesvirus type 1 ou OsHV-1. Les travaux réalisés au cours de ce stage ont contribué dans un premier temps à l'amélioration du protocole d'analyse basé sur l'utilisation d'un appareil de Polymerase Chain Reaction (PCR) en temps résel, puis aux analyses et au suivi de la charge virale par cette technique, d'individus entrant dans le cadre des projets - TRIPLOMER et AQUAFIRST-UE. Cette approche permettra par la suite de réaliser des diganostics fiables de la maladie tout en précisant le nombre de copies ADN (Acide...
Tipo: Text
Palavras-chave: Protocole; Herpes vrus; PCR quantitative; Huîtres creuses; Crassostrea gigas; Diagnostic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00033/14459/11761.pdf

Pathologie des mollusques bivalves et protection des cheptels coquilliers
Provedor de dados: 5
Autores: Grizel, Henri.
Shellfish farms show a number of specificities, some of which are decisive in the triggering and spreading of infectious and non-infectious diseases. This chapter deals only with infectious diseases which are currently the major hazard in shellfish culture, due to their virulence but also to the insufficiency of available fighting means. The epidemiological analysis is essentially based on the example of French shellfish culture. But the conclusions that can be drawn are of general interest. We then tackle the therapeutic and prophylactic fighting means. This study highlights the specificities of epizooties in bivalve molluscs and the constraints raised by fighting against farm diseases in an open and fluid environment,  farming habits and the consumption...
Tipo: Text
Palavras-chave: Mesures zootechniques; Traitement maladies; Prophylaxies zoosanitaires; Diagnostic; Maladies infectieuses; Maladies; Bivalves; Mollusques; Pathologie.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/1989/publication-6246.PDF

Bacterial diseases in marine bivalves
Provedor de dados: 5
Autores: Travers, Marie-agnes; Miller, Katherine Boettcher; Roque, Ana; Friedman, Carolyn S.
Bivalve aquaculture is seriously affected by many bacterial pathogens that cause high losses in hatcheries as well as in natural beds. A number of Vibrio species, but also members of the genera Nocardia and Roseovarius, are considered important pathogens in aquaculture. The present work provides an updated overview of main diseases and implicated bacterial species affecting bivalves. This review focuses on aetiological agents, their diversity and virulence factors, the diagnostic methods available as well as information on the dynamics of the host-parasite relationship.
Tipo: Text
Palavras-chave: Vibrio; Nocardia; Roseovarius; Rickettsia; Pathogenesis; Diagnostic.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00274/38532/37049.pdf

Guide méthodologique d’aide à la prise en compte des problématiques maritimes dès le diagnostic des SCOT côtiers
Provedor de dados: 5
Autores: Rodriguez, Thibault; Fourrier, Adeline; Mazouni, Nabila; Rey-valette, Helene; Roussillon, Jean Philippe; Laugier, Thierry; Loubersac, Lionel.
After a reminder on the program SYSCOLAG (Coastal and Lagoons Systems), of the regulatory and legislative context in territorial economic planning on the coast, we describe the structure of the developed methodological tool. We clarify then the method according to its three main trunk roads: effects of the activities and uses on the economy of the territory, the effects of the activities and uses on the natural environment and the living environment, the evaluation of the interactions between activities and uses. We apply the method exposed in a frame demonstrator by taking the scenario of the lagoon and coastal territory of the Thau Basin (Hérault). A set of appendices, including in particular evaluation grids are supplied with support for the...
Tipo: Text
Palavras-chave: Développement durable; Approche participative et pluridisciplinaire; SCOT; Volet Maritime; Diagnostic; Indicateur; Mer; Littoral; Impact environnemental; Effet socio-économique; Interaction; Activité; Usage.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00189/30058/28545.pdf

Transmission expérimentale du virus de type herpes chez l'huître creuse, Crassostrea gigas
Provedor de dados: 5
Autores: Arzul, Isabelle.
Au cours de l'été 1991, d'importantes mortalités de larves d'huître creuse, Crassostrea gigas, ont été observées dans des écloseries, simultanément en France (Comps et Cochennec, 1993) et en Nouvelle-Zélande (Hine &amp; al., 1992).  Les examens histologiques ainsi que des examens réalisés en microscopie électronique à transmission d'échantillons de larves présentant des mortalités ont permis de détecter la présence de particules virales associées à des lésions cellulaires.  D'après la morphologie, la taille, les localisations intranucléaires et cytoplasmiques du virus, il est possible d'envisager son appartenance à la famille des Herpesviridae.  De nouvelles mortalités sporadiques ont été observées au cours des étés suivants sur des lots de larves élevées...
Tipo: Text
Palavras-chave: Virus; Herpesviridae; Bivalve; Crassostrea gigas; Etiologie; Epidémiologie; Diagnostic; Reproduction infection.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00106/21711/19289.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 96 RPC R70 - Programme Pathologie - Rapport Scientifique année 1996
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Lipart, Cecile; Thebault, Anne.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00043/15452/12827.pdf

Short Technical Report for OsHV-1 detection and quantification by Real Time Polynerase Chain Reaction using OsHV-1 DNA polymerase sequence
Provedor de dados: 5
Autores: Pepin, Jean-francois.
The present report completes a previously described procedure routinely used for the detection and quantification of OsHV-1 by Sybr green Real Time Polymerase Chain Reaction targeting the OsHV-1 DNA polymerase sequence. More particularly, this document brings information about analytical sensitivity, quantitation limit, assay precision, reproducibility of the technique.
Tipo: Text
Palavras-chave: PCR quantitative HDP-F/R; Virus OsHV-1; Diagnostic; DNA polymerase; Validation; Real-Time PCR HDP-F/R; OsHV-1; Diagnostic; DNA polymerase; Validation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24814/22900.pdf

Development of a new diagnostic tool (Mini-Array) for ostreid Herpesvrus 1 (OsHV-1) detection in the Pacific oyster Crassostre gigas
Provedor de dados: 5
Autores: Varsamos, S.; Pepin, Jean-francois; Sauvage, Christopher; Renault, Tristan.
OsHV-1 was the first herpesvirus to be identified in an invertebrate host and is associated to mortalities in the Pacific oyster (Crassostrea gigas) and other economically important bivalve species. Infections due to OsHV-1 are frequently reported and cause high mortality rates in larvae and spat of C. gigas. Detection of this pathogen is usually performed by PCR and/or in situ hybridization. This presentation will focus on the development of a new molecular diagnostic tool for OsHV-1 (and OsHV-1 variant 1) detection: the mini-array. This detection system, which allows multiple pathogens detection, combines viral DNA amplification by PCR and a specific hybridisation on a nylon membrane, revealed by a colorimetric reaction (DNA chip). Multiplex PCR involves...
Tipo: Text
Palavras-chave: Diagnostic; Crassotrea gigas; Oyster; Detection; Herpesviridae; OsHV 1.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3652.pdf

Rapport de la mission effectuée du 12 au 19/12/97en Nouvelle-Calédonie : - Ecophysiologie et Pathologie
Provedor de dados: 5
Autores: Soyez, Claude; Avarre, Jean-christophe.
Ecophysiologie L'outil Capacité Osmorégulatrice (CO) mis au point ces dernières années est devenue un moyen performant et largement exploité en routine dans nos programmes de recherche en environnement, en pathologie-immunologie, en génétique et en physiologie de la nutrition. La mise en oeuvre de cet outil nous a permis de répondre à un certain nombre de questions posées dans le cadre de nos objectifs de recherche. Il s'agissait dans un deuxième temps d'éprouver et d'adapter cet outil à des études in situ (dans les bassins d'élevages). C'est dans ce cadre qu'une action a été menée en Nouvelle-Calédonie avec nos collègues de la SASV. En Nouvelle-Calédonie, la chute de température préside à une dégradation du milieu et des conditions d'élevage et...
Tipo: Text
Palavras-chave: Capacité osmorégulatrice; Stress; Environnement; Écophysiologie; Nouvelle-Calédonie; Diagnostic; V. penaeicida; Épidémiologie; PCR.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/00680/79233/81748.pdf

Recherches d'ADN d'hepès virus infectant les mollusques dans les populations d'huîtres en milieu naturel et dans leur environnement
Provedor de dados: 5
Autores: Pepin, Jean-francois; Solliec, Gaelle; Vigneron, Vassilia; Thebault, Anne; Lamothe, Julien; Renault, Tristan.
The presence of a herpes-type virus has been demonstrated in various species of marine shellfish (bivalves and gastropoda) throughout different regions of the world (Europe, North and South America, Asia, Australia), and is frequently associated with high mortality levels. Nevertheless, apart from the sanitary monitoring in mollusc farming, very little data is available about the detection of this type of virus in the natural populations of bivalves and about the persistence of such viruses in sea water. Various diagnostic tools have been developed in order to detect the oyster herpes virus (Ostreid herpesvirus, OsHV). The presence of this virus has been looked for by PCR in marine water samples coming from fattening ponds, in order to precise the natural...
Tipo: Text
Palavras-chave: Levures; Eau de mer; Diagnostic; Huîtres; Mollusques; OsHV; Herpèsvirus.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3716.pdf

Contribution à l'étude de mollusques marins : Iridovirus-like et Herpèsvirus-like. Description, caractérisation biochimique, cycle de multiplication viral, diagnostic et étude épidémiologique
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Among the marine molluscs, Pacifie oyster, Crassostrea gigas, is actually the most reared specie in the world. Noticely in France, where it was imported between 1960 and 1970, and replaced by Portuguese oyster, Crassostrea angulata, which specie was decimated during this period because of iridoviruses. Uncontroled introductions of new species may however be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases of bivalve molluscs in importing country. In addition to these observations, the quasi monobreeding of Pacific oyster in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this specie, namely the diagnosis of viral diseases. Several families of viruses...
Tipo: Text
Palavras-chave: Mollusques; Crassostrea gigas; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Molluscs; Crassostrea gigas; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00041/15230/12548.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 95 RPC R 62 - Programme Pathologie - Rapport Scientifique année 1995
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Thuillier, Benoit.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00043/15453/12828.pdf

VIBRance : éValuation des Impacts Bactériologiques dans l'Estuaire de la Rance. Hiérarchisation des flux de contamination microbiologique dans le cadre de l’élaboration du profil de vulnérabilité conchylicole du secteur Rance et baie de Saint-Malo
Provedor de dados: 5
Autores: Cheve, Julien; Le Noc, Sandrine.
Le projet VIBRance (éValuation des Impacts Bactériologiques dans l’estuaire de la Rance) a fait suite à l’observation d’une nette dégradation de la qualité microbiologique des eaux dans l’estuaire de la Rance (Rance maritime &amp; baie de Saint-Malo) au milieu des années 2010, présentant un risque pour la pérennité des activités tributaires : pêche à pied de loisir et professionnelle, conchyliculture, algoculture et à plus long terme la baignade. La situation revêt un caractère multi-sources (nombreux exutoires) et complexe (hydrodynamisme d’un estuaire découpé et contrôlé par un barrage marémoteur). La problématique posée est quelles sont les sources de contamination microbiologique dans l’estuaire de la Rance et leurs impacts associés ? Le projet...
Tipo: Text
Palavras-chave: Impact; Bactériologique; Microbiologique; Estuaire; Rance; Baie de Saint- Malo; Profil; Vulnérabilité; Conchylicole; Baignade; Pêche; Pied; Escherichia coli; Diagnostic; Courantologie; Modélisation; Dispersion source; Contributeur; VIBRance.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00442/55363/56879.pdf

Note de synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Avril-Mai 1996
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile; Le Deuff, Rose-marie; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade dans le cadre de l'étude du virus de type herpès et de la mise au point de méthodes de diagnostic fait suite à la précédente note du 16 avril 1996. Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont donnés et commentés en sept points. 1- Analyses Pays de Loire 2 - Bilan des analyses réalisées dans le cadre du protocole IFREMER BOUIN/IFREMER ARGENTON/SODABO 3 - Bilan global des analyses Pays de Loire et Bouin-Argenton-SODABO 4 - Analyses effectuées en PCR pour la recherche de virus de type herpès sur des lots prélevés en 1996, dans le...
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15499/12867.pdf

Herpèsvirus infectant les bivalves marins : détection, génome et transmission
Provedor de dados: 5
Autores: Arzul, Isabelle.
Since 1991, high mortality rates of larvae and juveniles of different bivalve mollusc species associated with herpesvirus infections have been reported in different countries. The aim of this study was to determine if more than one helpesvirus species was responsible for these infections or just one species exhibiting polymorphism. The investigations concentrated on the analysis of the virus genome, studies of virus transmission in different mollusc species and on the potential role of bivalve adults in the occurrence of infections. The analysis of various target sequences within the virus genome present in infected larvae samples demonstrated the presence of polymorphism. More notably a variant of the virus was identified and the variation appears...
Tipo: Text
Palavras-chave: Herpèsvirus; Bivalves; Transmission; Polymorphisme; OsHV-1; Diagnostic; Herpesviruses; Bivalvia; Transmission; Polymorphism; OsHV-1; Diagnostic.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00105/21675/19253.pdf

Contribution à l'étude de virus de mollusques marins apparentés aux Iridoviridae et aux Herpesviridae
Provedor de dados: 5
Autores: Le Deuff, Rose-marie.
Among the marine molluscs, Pacific oyster, Crassostrea gigas, is actually the most reared species in the world. Noticeably in France, where it was imported between 1960 and 1970, and replaced Portuguese oyster, Crassostrea angulata. Intensification of breeding and the associated introductions of new species may be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases in bivalve molluscs. In addition to these observations, the quasi monobreeding of C. gigas in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this species, namely the diagnosis of viral diseases. Several families of viruses have been described within marine bivalves molluscs. The purpose of this...
Tipo: Text
Palavras-chave: Mollusques bivalves; Crassostrea gigas; Huître japonaise; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Bivalve molluscs; Crassostrea gigas; Pacific oyster; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00107/21792/19367.pdf

Réseau de Suivi Lagunaire du Languedoc-Roussillon. Bilan des résultats 2002
Provedor de dados: 5
Autores: Laugier, Thierry; Fiandrino, Annie; Souchu, Philippe; Benau, Laurent.
The numerous coastallagoons lining the Languedoc-Roussillon mediterranean coast undergone high anthropic pressure. The Lagoon Monitoring Network as a result of partnership between Ifremer, the Languedoc- Roussillon Regional Council and the Rhône Méditerranée Corse Water Agency, apply to provide yearly a diagnostic of the environmental quality of21lagoons. This network has two main goals: • The monitoring of the state of the lagoon with regards to eutrophication and sorne specifie survey in coordination with local managers. • Management support (model simulation, synthetic infonnation, scientific support) towards local managers. This annual report gathers results of the actions planned in 2002 within the Network : • For each lagoon excosystem, results of...
Tipo: Text
Palavras-chave: Lagunes littorales; Méditerranée; Eutrophisation; Diagnostic; Surveillance; Gestion; Coastal lagoon; Mediterranean; Eutrophication; Diagnostic; Monitoring; Management.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00419/53085/54046.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 98 RPC R 7 - Programme Pathologie - Rapport Final année 1998
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf

Etude de l'herpès virus de l'huître. Fonds d'Aide à la Recherche et à l'Innovation. Décision d'Aide à la Recherche n° 95-07-01 (Rapport intermédiaire) 1997
Provedor de dados: 5
Autores: Renault, Tristan.
Le Laboratoire de Génétique, Aquaculture et Pathologie (GAP) de la Station IFREMER de La Tremblade, se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occasionne. Les diagnostics proposés seront basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR : Polymerase Chain Reaction). Ce projet, proposé sur une durée de trois années...
Tipo: Text
Palavras-chave: Virus; Herpès virus; Crassostrea gigas; Pathogènes; Diagnostic; Herpesviridae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00043/15448/12821.pdf

Outils de dépistage des pathogènes - Contrat Région Poitou-Charentes 1999 - Convention 99/RPC-A-200 "Pathologie"
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie.
Le Laboratoire de Génétique et Pathologie (LGP) de la station IFREMER de La Tremblade (Charente Maritime) est impliqué aux plans national et international dans l'étude des maladies affectant les mollusques bivalves marins d' intérêt économique et plus particulièrement les huîtres. Des travaux concernant le développement, la validation et l'utilisation d'outils de diagnostic spécifiques d'agents infectieux propres aux coquillages y sont ainsi menés.
Tipo: Text
Palavras-chave: Pathologie; Maladie; Mollusques bivalves; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00043/15471/12850.pdf

Synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Juin-Septembre 1996
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type . herpès et de la mise au point des méthodes de diagnostic fait suite à la précédente note (fin mai 1996). Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont données et commentés en quatre points. Cette étude est soutenue par la Région Poitou-Charente et la Région Pays de Loire.
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Infection; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15503/12875.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 97 RPC R 58 - Programme Pathologie - Rapport Scientifique année 1997
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Cochennec, Nathalie.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00043/15454/12829.pdf

Contribution à l'étude de vibrions pathogènes d'huîtres creuses Crassostrea gigas - Interactions hémocytes-vibrions et développement de tests de diagnostic d'intérêt taxonomique
Provedor de dados: 5
Autores: Duperthuy, Marylise.
Les bactéries du genre Vibrio représentent le groupe le plus fréquemment associé à des maladies infectieuses chez les mollusques marins et notamment l'huître creuse Crassostrea gigas. A ce jour trois espèces de Vibrio pathogènes d'huîtres creuses V. splendidus, V. aestuarianus et récemment V. coralliitycus ont été identifiés en France. La prévention des mortalités associées à ces vibrioses repose sur une meilleure compréhension des interactions hôte-pathogène et sur l'utilisation de techniques de diagnostic rapides à mettre en oeuvre et fiables. Ce travail a permis d'une part de caractériser la dynamique de phagocytose de vibrions par les cellules immunocompétentes de l'huître, les hémocytes. La mise au point d'un protocole d'analyse en cytométrie de flux...
Tipo: Text
Palavras-chave: Huître creuse; Crassostrea gigas; Vibrio splendidus; Vibrio aestuarianus; Vibrio corralliitycus; Hémocytes; Phagocytose; Cytométrie de flux; Diagnostic; PCRq.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00033/14452/11753.pdf

Session de formation à l'utilisation de la technique PCR pour le diagnostic de l'infection à Herpèsvirus chez les huîtres
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile.
Parmi les maladies infectieuses observées chez les mollusques bivalves marins, les viroses sont souvent mal connues, en raison d'une certaine inadéquation des techniques d'identification et de diagnostic généralement mises en oeuvre lors de phénomènes de mortalité. En effet, la microscopie photonique a été et reste encore, dans de nombreux laboratoires travaillant sur les pathologies des mollusques, la technique de base pour l'analyse des échantillons. Cette technique reste insuffisante en cas de pathologie d'origine virale si elle n'est pas complétée par d'autres approches (microscopie électronique à transmission, recherche d'effets cytopathogènes sur monocouches cellulaires et réactifs de diagnostic spécifiques). Aucune lignée de mollusques bivalves...
Tipo: Text
Palavras-chave: Pathologie; Technique laboratoire; PCR; Diagnostic; Herpèsvirus; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15501/12870.pdf

Validation of in situ hybridisation and histology assays for the detection of the oyster parasite Marteilia refringens
Provedor de dados: 5
Autores: Thebault, Anne; Bergman, S; Pouillot, R; Le Roux, Frédérique; Berthe, Franck.
An in situ hybridisation technique has been developed for the detection of infection in oysters with Marteilia refringens with particular emphasis on light infections or confirmation of suspected cases by means of histology. Although validation of new diagnostic methods is usually achieved by comparison with standard techniques, in our case the sensitivity and specificity of the standard (histology) had not previously been established. Another point to consider is that surveillance and monitoring frequently target populations displaying different levels of prevalence under different field conditions. The objective of our study was to evaluate the sensitivity and specificity values of in situ hybridisation and histology for the detection of M refringens,...
Tipo: Text
Palavras-chave: Ostrea edulis; Marteilia refringens; Latent models; Specificity; Sensitivity; Validation; Diagnostic.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3624.pdf

Evaluation du stock de coquilles Saint-Jacques de la Baie de Seine et du Proche Extérieur. COMOR 32 (8-23 juillet 2002)
Provedor de dados: 5
Autores: Foucher, Eric.
Scientific scallop surveys called COMOR are carried out in the bay of Seine since 1976. Results are used by fishermen’ associations and by fishing administration to lead management measures. In this report, the scientific results of the survey COMOR 32 realised in July 2002 are described. Abundance and growth indices for scallop, by age and area, are presented. During these surveys, data about most abundant benthic species are also collected since 1998. A first assessment with five years data is made here. A global descriptive analysis is undertaken about all the species present on scallops grounds. A special zoom is applied on both species whitch could be exploited (whelk Buccinum undatum and queen scallop Aequipecten opercularis) and three competitive...
Tipo: Text
Palavras-chave: Coquille Saint-Jacques; Pecten maximus; Baie de Seine; Évaluation directe; Stock; Diagnostic; Scallop survey; Pecten maximus; Bay of Seine; Assessment; Stock.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00341/45178/44575.pdf

Molecular identification of Vibrio tapetis, the causative agent of the brown ring disease of Ruditapes philippinarum
Provedor de dados: 5
Autores: Paillard, Christine; Gausson, Sabrina; Nicolas, Jean-louis; Le Pennec, Jean Paul; Haras, Dominique.
Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V...
Tipo: Text
Palavras-chave: DOT BLOT hybridisation; SSP PCR; Vibriosis; Bivalve; Detection; Diagnostic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1692.pdf

Pathologie des invertébrés marins d'intérêt aquacole. Contribution et stratégies de recherches
Provedor de dados: 5
Autores: Mialhe, Eric.
Les productions d'invertébrés sont prépondérantes en aquaculture marine qui est économiquement très importate pour un grand nombre de pays, notamment de la zone Sud. Cette activité est confrontée à de nombreux problèmes des pathologie qui remettent en cause sa périnnité et son développment. Jusque dans les années quatre-vingt, les travaux relatifs à la pathologie des mollusques et des crevettes ont essentiellement porté sur la description des agents pathogènes. La création en 1985 par l'ISTPM/IFREMER d'un laboratoire pourvu de tous les équipements nécessaires aux techniques de pathologie a permis de mettre en place un programme de recherches orienté vers le contrôle des maladies. Une double stratégie de travail a été alors défine pour apporter  à...
Tipo: Text
Palavras-chave: Pathologie; Immmunologie; Génétique; Mollusque; Crevette; Diagnostic; Epidémiologie; Traitement; Sélection; Transformation génétique.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00443/55479/57009.pdf

Rapid PCR-based assay for Sclerotinia sclerotiorum detection on soybean seeds
Provedor de dados: 63
Autores: Grabicoski,Edilaine Mauricia Gelinski; Jaccoud Filho,David de Souza; Pileggi,Marcos; Henneberg,Luciane; Pierre,Marcelo Luiz Cunha; Vrisman,Cláudio Mauricio; Dabul,Audrei Nisio Gebieluca.
Caused by Sclerotinia sclerotiorum, white mold is an important seed-transmitted disease of soybean (Glycine max). Incubation-based methods available for the detection and quantification of seed-borne inoculum such as the blotter test, paper roll and Neon-S assay are time-consuming, laborious, and not always sensitive. In this study, we developed and evaluated a molecular assay for the detection of S. sclerotiorum in soybean seeds using a species-specific PCR (polymerase chain reaction) primer set and seed soaking (without DNA extraction) for up to 72 h. The PCR products were amplified in all the samples infected with the pathogen, but not in the other samples of plant material or the other seed-borne fungi DNA. The minimum amount of DNA detected was 10 pg,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: White mold; Dissemination; Soaking seeds; Diagnostic.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100069

PET and PET/CT imaging for the earliest detection and treatment of colorectal carcinoma
Provedor de dados: 52
Autores: Carter,Kevin; Kotlyarov,Eduard.
Approximately 150,000 new cases of colorectal cancer are diagnosed each year with the life time risk of developing colon caner in developed nations being 4.6% in men and 3.2% in women. Screening patients is essential early detection of colon carcinoma to aid in complete resection. Unfortunately current screening methods carry with them poor patient compliance. PET and PET/CT may be a significant part of this screening solution. The authors reviewed and analyzed the English language articles and case reports identified on Medline during the last 10 years. PET and PET/CT results for colorectal carcinoma were tabulated and presented for the fifth Scientific Meeting of the Brazilian Society of Nuclear Biosciences. Though most studies have been retrospective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PET and PET/CT; Colorectal cancer; Diagnostic.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700025

A Rapid and Reliable Method for Molecular Detection of Fusarium guttiforme, the Etiological Agent of Pineapple Fusariosis
Provedor de dados: 52
Autores: Carnielli-Queiroz,Lorena; Fernandes,Patricia Machado Bueno; Fernandes,Antônio Alberto Ribeiro; Ventura,José Aires.
Abstract Pineapple (Ananas comosus var. comosus) fusariosis is an economically important fungal disease affecting the plant and its fruit. A rapid and reliable diagnosis is the base of integrated disease management practices. Fusariosis has resulted in quarantines for pineapple products in Central America, Africa and Asia. Difficulties diagnosing and correctly identifying the fungus Fusarium guttiforme, agent of the pineapple fusariosis, have led to the search for new methodologies, and for this we developed a new reliable molecular method to detect it. For diagnostic purposes, real-time PCR of elongation factor gene 1-α (ef1) was used to rapidly, specifically and sensitively diagnose F. guttiforme. A pathogenicity test was conducted with slips of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnostic; Diseases; Quarantine; Real-time PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100219

Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4
Provedor de dados: 52
Autores: Krüger,Ernesto Renato; Penha,Tânia Regina; Hummelgen,Fernanda Cristina; Agottani,Jorge Bacila; Reva,Demétrio; Gonçalves,Robson; Thomaz-Soccol,Vanete.
ABSTRACTThe aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-4; Serology; Diagnostic; Cattle; Epidemiology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500725

Molecular epidemiology of the human group A rotavirus in the Paraná State, Brazil
Provedor de dados: 52
Autores: Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo.
From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008

Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples
Provedor de dados: 55
Autores: de Filippis,Ivano; Andrade,Claudia Ferreira de; Caldeira,Nathalia; Azevedo,Aline Carvalho de; Almeida,Antonio Eugenio de.
Abstract  Background Several in-house PCR-based assays have been described for the detection of bacterial meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae from clinical samples. PCR-based methods targeting different bacterial genes are frequently used by different laboratories worldwide, but no standard method has ever been established. The aim of our study was to compare different in-house and a commercial PCR-based tests for the detection of bacterial pathogens causing meningitis and invasive disease in humans.  Methods A total of 110 isolates and 134 clinical samples (99 cerebrospinal fluid and 35 blood samples) collected from suspected cases of invasive disease were analyzed. Specific sets of primers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnostic; Neisseria meningitidis; Streptococcus pneumoniae; Haemophilus influenzae.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400335

Rapid detection of Vancomycin-Resistant Enterococci (VRE) in rectal samples from patients admitted to intensive care units
Provedor de dados: 55
Autores: d'Azevedo,Pedro Alves; Santiago,Kelly Aline de Souza; Furtado,Guilherme Henrique Campos; Xavier,Diego Batista; Pignatari,Antonio Carlos Campos; Titze-de-Almeida,Ricardo.
The reduction in time required to identify vancomycin-resistant enterococci (VRE) has gained increased importance during hospital outbreaks. In the present study, we implemented a laboratory protocol to speed up the VRE screening from rectal samples. The protocol combines a medium for selective VRE isolation (VREBAC®, Probac, São Paulo) and a multiplex PCR for detection and identification of vanA and vanB resistance genes. The screening performance was analyzed in 114 specimens collected from four intensive care units. The swabs were collected at two periods: (1) during a VRE outbreak (February 2006, n=83 patients) and (2) at the post-outbreak period, after adoption of infection control measures (June 2006, n=31 patients). Forty-one/83 VRE (49.4%) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterococcus; VRE; Vancomycin; Diagnostic; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400010

Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis
Provedor de dados: 56
Autores: López-López,P.; Martínez-López,M.C.; Boldo-León,X.M.; Hernández-Díaz,Y.; González-Castro,T.B.; Tovilla-Zárate,C.A.; Luna-Arias,J.P.
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amebiasis; Neglected diseases; Diagnostic; PCR; DGGE; Adh112 gene.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607

Leptospirosis in slaughtered sows: serological and histopathological investigation
Provedor de dados: 58
Autores: Delbem,Ádina Cléia Botazzo; Freitas,Julio Cesar de; Bracarense,Ana Paula F. R. L.; Müller,Ernst Eckehardt; Oliveira,Rosângela Claret de.
Swine leptospirosis is recognized world wide as an important cause of reproductive failure. Serum and histo-pathological examinations have been constantly used to diagnose this disease. This study was carried out on slaughtered sows from Northern Paraná State to compare serum findings with kidney, liver, ovary and uterus histo-pathological examinations. The microscopic serum-agglutination carried out on 36 animals detected 24 with titles &gt; 100 and 12 negative (&lt; 100). The histopathological examinations made on livers, kidneys, ovaries and uterus stained with hematoxylin-eosin, were carried out on 16 randomly chosen animals, 11 from the groups with &gt; 100 serological titles and five from the negative group. The results showed that all the 16 animals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Swine; Diagnostic.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200016

Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
Provedor de dados: 58
Autores: Cargnelutti,Juliana Felipetto; Brum,Mário Celso Sperotto; Weiblen,Rudi; Flores,Eduardo Furtado.
West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: West Nile virus; WNV; M/E glycoproteins; Stable expression; Diagnostic.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040

Mycoplasmas hyorhinis in different regions of cuba: diagnosis
Provedor de dados: 58
Autores: Lobo,Evelyn; Poveda,Carlos; Suarez,Alejandro; Hernández,Yenney; Ramírez,Ana; Poveda,José B.
M. hyorhinis is considered one of the etiological agents of arthritis in sucking pigs, but recently as seen, some strains can produce pneumonia that could not be distinguished from the mycoplasmosis caused by M. hyopneumoniae. The study was conducted to research the presence of Mycoplasma hyorhinis (M. hyorhinis ) in different regions of the country from exudates of pig lungs with typical EP lesions. Exudates from 280 pig lungs with typical EP lesions were studied using molecular techniques such as PCR, real time PCR and amplification of the 16S-23S rRNA. It was detected that the 66% of the samples studied resulted positive to M. hyorhinis, and the presence of this species was detected in all the provinces. Amplification and studies on the intergenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Mycoplasma; Diagnostic; Enzootic pneumonia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200039

Rapid serum agglutination and agar gel immunodiffusion tests associated to clinical signs in rams experimentally infected with Brucella ovis
Provedor de dados: 65
Autores: Nozaki,Cristiane Nakada; Lira,Nair Silva Cavalcanti de; Augusto Filho,Otávio; Azevedo,Hymerson Costa; Rodello,Leandro; Dasso,Maurício Gaudério; Bicudo,Sony Dimas; Antunes,João Marcelo Azevedo de Paula; Megid,Jane.
The purpose of this study was to evaluate the agar gel immunodiffusion and the rapid serum agglutination tests associated to clinical signs in rams experimentally infected with Brucella ovis. The serological profile during the 12 months of infection showed a large fluctuation of antibodies that favors the failure in the diagnostic. The evaluation of tests after the experimental infection allowed to suggest that none of the tests were able to detect the infection throughout the period of study. The study reinforces the importance of considering the clinical signs to support the diagnosis of Brucella ovis infection in rams.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovine infectious epididimitis; Sheep brucellosis; Diagnostic.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800023

Virus isolation in cell culture for confirmatory diagnostic of rabies in bovine specimens
Provedor de dados: 65
Autores: Kanitz,Fabio Adriano; Cargnelutti,Juliana Felipetto; Weiblen,Rudi; Batista,Helena Beatriz de Carvalho Ruthner; Flores,Eduardo Furtado.
ABSTRACT: This study investigated the suitability of virus isolation (VI) in mouse neuroblastoma cells (N2A) and baby hamster kidney cells (BHK-21) as a confirmatory test for diagnosis of bovine rabies. Fourty-eight brain samples from cattle suspected of rabies were initially submitted to fluorescent antibody test (FAT) and mouse inoculation test (MIT) for routine diagnostic. Subsequently, these specimens were submitted to three protocols of VI in each cell line: a single 24h or 72h passage (T1, T2), or three 48h passages (T3). The FAT and MIT combined detected 32/48 positive samples, from which MIT detected 32 and FAT 31. The average time required for final MIT results was 12.3 days (8 - 21). VI in BHK-21 cells provided definitive, positive results in...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Rabies; Cattle; Outbreaks; Diagnostic; Virus isolation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202193

Epidemiology of Mycoplasma agalactiae and Mycoplasma mycoides cluster in flocks of northeastern Brazil
Provedor de dados: 65
Autores: Santos,Sandra Batista dos; Melo,Renata Pimentel Bandeira de; Silva,Luana Thamires Rapôso da; Oliveira,Júnior Mário Baltazar de; Abad,Atzel Candido Acosta; Pinheiro Júnior,José Wilton; Mota,Rinaldo Aparecido.
ABSTRACT: The present study aimed to investigate contagious agalactia (CA) in flocks from Pernambuco State. The study involved 225 goats and 63 ewes; 288 milk samples and 100 vaginal swabs were collected in total. The PCR assays were carried out using specific primers to Mycoplasma agalactiae and the Mycoplasma mycoides cluster. Among the goat’s milk samples,12.0% (27/225) were positive for Mycoplasma agalactiae DNA, while 5.3% (12/225) contained the Mycoplasma mycoides cluster. Of the vaginal swabs taken from goats, 15.4% (12/78) were positive for Mycoplasma agalactiae DNA and 3.8% (3/78) contained the Mycoplasma mycoides cluster. In the case of ewes, 4.3% (1/23) of the milk samples contained Mycoplasma agalactiae DNA, and 7.5% (3/40) were positive for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contagious agalactia; Flocks; Diagnostic; Epidemiology; Risk factor.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000400452

Isolation of Leptospira spp from dogs, bovine and swine naturally infected
Provedor de dados: 65
Autores: Freitas,Julio Cesar de; Silva,Francielle Gibson da; Oliveira,Rosângela Claret de; Delbem,Ádina Cléia Botazzo; Müller,Ernst Eckehardt; Alves,Lucimara Aparecida; Teles,Paulo Sergio.
Leptospira isolation allows definitive diagnosis of the infection. Contamination by microorganisms is one of the inconveniences of the culture. The objective of this study was to describe the isolation of Leptospira from dogs, bovine and swine naturally infected. Urine samples from 14 dogs and three bovines, and kidney, liver, ovary, and uterus body samples from 36 slaughtered sows with unknown health history, were used. The urine and organ samples were cultured in culture medium. Modified Ellinghausen-McCullough-Johnson-Harris medium (EMJH) culture medium was used with addition of 5-fluorouracil, chloramphenicol, vancomycin, nalidixic acid and neomycin. Incubation was performed at 28oC for 24 hours, followed by subculture in modified EMJH without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Diagnostic; Culture; Cattle; Dog; Swine.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300030

Feline subcutaneous pythiosis
Provedor de dados: 65
Autores: Soares,Luciana Maria Curtio; Schenkel,Diego Montagner; Rosa,Janaina Marcela Assunção; Azevedo,Ludmila Silva; Tineli,Tainara Renata; Dutra,Valéria; Colodel,Edson Moleta; Pescador,Caroline Argenta.
ABSTRACT:  Pythiosis in felines is a rare disease associated with the oomycete Pythium insidiosum. The aim of this report was to describe the macroscopic, histopathological, and molecular characteristics of P. insidiosum infection in a 2-year-old cat, with a localized invasive subcutaneous mass. The feline had an increase of volume near the anal region since it was younger. The cat died just after surgery. The necropsy was performed, and samples were collected for histopathological examination. Microscopically, the skin lesion was characterized by necro-eosinophilic dermatitis, panniculitis, and myositis surrounding negatively stained hyphal structures. In the sections stained with GMS, dark brown hyphae were clearly seen inside the affected tissue. They...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pythium insidiosum; Diagnostic; Cat.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300450