Consulta: subjectFacets:"Differential expression"
Registros recuperados: 12
Data/hora: 03/06/2024 03:31:21

Introduction of the rd29A: AtDREB2A CA gene into soybean (Glycine max L. Merril) and its molecular characterization in leaves and roots during dehydration
Provedor de dados: 74
Autores: Engels,Cibelle; Fuganti-Pagliarini,Renata; Marin,Silvana Regina Rockenbach; Marcelino-Guimarães,Francismar Corrêa; Oliveira,Maria Cristina Neves; Kanamori,Norihito; Mizoi,Junya; Nakashima,Kazuo; Yamaguchi-Shinozaki,Kazuko; Nepomuceno,Alexandre Lima.
The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drought. The abscisic acid (ABA)-independent Dehydration Responsive Element Binding (DREB) gene family has been used to obtain plants with increased tolerance to abiotic stresses. In the present study, the rd29A:AtDREB2A CA gene from Arabidopsis thaliana was inserted into soybean using biolistics. Seventy-eight genetically modified (GM) soybean lines containing 2-17 copies of the AtDREB2A CA gene were produced. Two GM soybean lines (P1397 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis; Differential expression; Genetically modified organism; Water deficit.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400015

Differential gene expression profiles in peripheral blood in Northeast Chinese Han people with acute myocardial infarction
Provedor de dados: 74
Autores: Fan,Lin; Meng,Heyu; Guo,Xudong; Li,Xiangdong; Meng,Fanbo.
Abstract This study aimed to use gene chips to investigate differential gene expression profiles in the occurrence and development of acute myocardial infarction (AMI). The study included 12 AMI patients and 12 healthy individuals. Total mRNA of peripheral bloodwas extracted and reversed-transcribed to cDNA for microarray analysis. After establishing two pools with three subjects each (3 AMI patients and 3 healthy individuals), the remaining samples were used for RT-qPCR to confirm the microarray data. From the microarray results, seven genes were randomly selected for RT-qPCR. RT-qPCR results were analyzed by the 2-ΔΔCt method. Microarray analysis showed that 228 genes were up- regulated and 271 were down-regulated (p ≤ 0.05, |logFC| &gt; 1). Gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myocardial infarction; RNA; Differential expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100059

In silico evaluation of the Eucalyptus transcriptome
Provedor de dados: 74
Autores: Vicentini,Renato; Sassaki,Flávio T.; Gimenes,Marcos A.; Maia,Ivan G.; Menossi,Marcelo.
The expressed sequence tags (ESTs) produced in the Forests project provide an invaluable opportunity to assess the Eucalyptus transcriptome. Besides providing information on the different proteins produced by this plant, it is possible to infer gene expression profiles because non-normalized cDNA libraries were used. The EST frequency from any gene is correlated to the transcript levels in the tissues from which the cDNA libraries were constructed. The goal of this work was to identify Eucalyptus genes that showed either differential expression pattern or were ubiquitously expressed in the tissues sampled in the Forests project. Six robust statistical tests and very restrictive rules were applied to gain confidence in the in silico data aiming to avoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transcriptome; Eucalyptus; Tissue-specific; Statistics; Differential expression.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400002

Identification of differentially expressed genes of Xanthomonas axonopodis pv. citri by representational difference analysis of cDNA
Provedor de dados: 74
Autores: Mehta,Angela; Rosato,Yoko B.
Xanthomonas axonopodis pv. citri is a phytopathogenic bacterium responsible for citrus canker, a serious disease which causes severe losses in citriculture around the world. In this study we report the differential expression of X. axonopodis pv. citri in response to specific treatments by using Representational Difference Analysis of cDNA (cDNA RDA). cDNAs from X. axonopodis pv. citri cultured in the presence of leaf extract of the host plant (Citrus sinensis), in vivo, as well as in the complex medium were hybridized against cDNA of the bacterium grown in the minimal medium. Sequencing of the difference products obtained after the second and third hybridizations revealed a total of 37 distinct genes identified by homology searches in the genome of X....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus canker; Differential expression; Leaf extract; In vivo.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100024

Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
Provedor de dados: 74
Autores: Maluf,Mirian Perez; Silva,Carla Cristina da; Oliveira,Michelle de Paula Abreu de; Tavares,Aline Gomes; Silvarolla,Maria Bernadete; Guerreiro Filho,Oliveiro.
In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Cup quality; Differential expression; Naturally decaffeinated.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400023

Bioinformatics analysis of the gene expression profile in Bladder carcinoma
Provedor de dados: 74
Autores: Xiao,Jing; Yiqing,Qiu.
Bladder carcinoma, which has the ninth highest incidence among malignant tumors in the world, is a complex, multifactorial disease. The malignant transformation of bladder cells results from DNA mutations and alterations in gene expression levels. In this work, we used a bioinformatics approach to investigate the molecular mechanisms of bladder carcinoma. Biochips downloaded from the Gene Expression Omnibus (GEO) were used to analyze the gene expression profile in urinary bladder cells from individuals with carcinoma. The gene expression profile of normal genomes was used as a control. The analysis of gene expression revealed important alterations in genes involved in biological processes and metabolic pathways. We also identified some small molecules...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bladder carcinoma; Differential expression; Expression profile; Small molecular mimic; Susceptibility forecast.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200021

Gonad transcriptome analysis of pearl oyster Pinctada margaritifera: identification of potential sex differentiation and sex determining genes
Provedor de dados: 5
Autores: Teaniniuraitemoana, Vaihiti; Huvet, Arnaud; Levy, Peva; Klopp, Christophe; Lhuillier, Emeline; Gaertner-mazouni, Nabila; Gueguen, Yannick; Le Moullac, Gilles.
Background  Black pearl farming is based on culture of the blacklip pearl oyster Pinctada margaritifera (Mollusca, lophotrochozoa), a protandrous hermaphrodite species. At first maturation, all individuals are males. The female sex appears progressively from two years old, which represents a limitation for broodstock conditioning for aquaculture production. In marine mollusks displaying hermaphroditic features, data on sexual determinism and differentiation, including the molecular sex determining cascade, are scarce. To increase genomic resources and identify the molecular mechanisms whereby gene expression may act in the sexual dimorphism of P. margaritifera, we performed gonad transcriptome analysis.  Results  The gonad transcriptome of P. margaritifera...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Gametogenesis; Transcriptome; Differential expression; Sex determinism.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00201/31194/29593.pdf

Identification of genes associated with shell color in the black-lipped pearl oyster, Pinctada margaritifera
Provedor de dados: 5
Autores: Lemer, Sarah; Saulnier, Denis; Gueguen, Yannick; Planes, Serge.
Background: Color polymorphism in the nacre of pteriomorphian bivalves is of great interest for the pearl culture industry. The nacreous layer of the Polynesian black-lipped pearl oyster Pinctada margaritifera exhibits a large array of color variation among individuals including reflections of blue, green, yellow and pink in all possible gradients. Although the heritability of nacre color variation patterns has been demonstrated by experimental crossing, little is known about the genes involved in these patterns. In this study, we identify a set of genes differentially expressed among extreme color phenotypes of P. margaritifera using a suppressive and subtractive hybridization (SSH) method comparing black phenotypes with full and half albino individuals....
Tipo: Text
Palavras-chave: Differential expression; Biomineralization; Nacre; Pearl; Pigmentation; Albino.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00275/38627/37167.pdf

Changes in fatty acid composition in the giant clam Tridacna maxima in response to thermal stress
Provedor de dados: 5
Autores: Dubousquet, Vaimiti; Gros, Emmanuelle; Berteaux-lecellier, Veronique; Viguier, Bruno; Raharivelomanana, Phila; Bertrand, Cédric; Lecellier, Gael J.
Temperature can modify membrane fluidity and thus affects cellular functions and physiological activities. This study examines lipid remodelling in the marine symbiotic organism, Tridacna maxima, during a time series of induced thermal stress, with an emphasis on the morphology of their symbiont Symbiodinium. First, we show that the French Polynesian giant clams harbour an important proportion of saturated fatty acids (SFA), which reflects their tropical location. Second, in contrast to most marine organisms, the total lipid content in giant clams remained constant under stress, though some changes in their composition were shown. Third, the stress-induced changes in fatty acid (FA) diversity were accompanied by an upregulation of genes involved in lipids...
Tipo: Text
Palavras-chave: Thermal stress; Fatty acids; Differential expression; Antioxidant; Symbiodinium; Tridacna maxima.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00361/47195/79514.pdf

Transcriptional analyses of Trypanosoma brucei gambiense from infected mice and in vitro culture
Provedor de dados: 19
Autores: Kuboki, Noritaka; Kibe, Michael K; Thekisoe, Oriel M. M.; Sugimoto, Chihiro; Inoue, Noboru.
With the hypothesis that African trypanosomes could have in vivo specific genes for adaptation to host’s environment, the present study was conducted by using suppressive subtractive hybridization (SSH) technique to seek the highly expressed genes especially in host. A total of 328 clones from the in vivo SSH library and that of 160 clones from the in vitro SSH library were analyzed in order to determine their expression levels, but none of the above-mentioned genes showed differential expression. This indicates that no trypanosome genes could be differentially expressed either the in vivo or in vitro propagated trypanosomes. Alternatively, there might be limitation for detecting specifically expressed genes in African trypanosomes using this method,...
Palavras-chave: Differential expression; Infection; Trypanosoma brucei.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1052

Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages
Provedor de dados: 86
Autores: Gaspari-Pezzopane,Cristiana de; Bonturi,Nemailla; Guerreiro Filho,Oliveiro; Favarin,José Laércio; Maluf,Mirian Perez.
The objective of this work was to identify genes that could be used as suitable markers for molecular recognition of phenological stages during coffee (Coffea arabica) fruit development. Four cultivars were evaluated as to their differential expression of genes associated to fruit development and maturation processes. Gene expression was characterized by both semi-quantitative and quantitative RT-PCR, in fruit harvested at seven different developmental stages, during three different seasons. No size polymorphisms or differential expression were observed among the cultivars for the evaluated genes; however, distinct expression profiles along fruit development were determined for each gene. Four out of the 28 evaluated genes exhibited a regular expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Cup quality; Differential expression; Fruit phenology; Ripening; QRT-PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000700014

HIGH EXPRESSION OF HMOX1 IN BLUE-SHELLED CHICKENS IS ASSOCIATED WITH A TG HAPLOTYPE
Provedor de dados: 89
Autores: Wang,Z; Deng,X; Wang,AI; Liu,R.
ABSTRACTHMOX1 is an important gene in biosynthesis of the eggshell pigment of blue eggs. Previous studies found that HMOX1 is highly expressed in the shell gland of hens laying blue eggs (BlueH) compared with hens laying brown eggs (BrownH); however, the reasons for the differential expression are unclear. In this study five single nucleotide polymorphism (SNP) in HMOX1 were genotyped in 111 BlueH and 115 BrownH. The association of haplotypes of these SNP with the blue egg phenotype was tested. Haplotype-specific expression of HMOX1 was detected in the shell gland. The interaction of sequence variants and transcription factors was analyzed using electrophoretic mobility shift assay (EMSA). A TG haplotype covering upstream 1.4 kb region of HMOX1 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Blue egg; Biliverdin; HMOX1; Differential expression.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000300267