Consulta: subjectFacets:"Differential vaccine"
Registros recuperados: 3
Data/hora: 13/06/2024 08:32:41

A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain
Provedor de dados: 56
Autores: Weiss,M.; Brum,M.C.S.; Anziliero,D.; Weiblen,R.; Flores,E.F.
A bovine herpesvirus 1 (BoHV-1) defective in glycoprotein E (gE) was constructed from a Brazilian genital BoHV-1 isolate, by replacing the full gE coding region with the green fluorescent protein (GFP) gene for selection. Upon co-transfection of MDBK cells with genomic viral DNA plus the GFP-bearing gE-deletion plasmid, three fluorescent recombinant clones were obtained out of approximately 5000 viral plaques. Deletion of the gE gene and the presence of the GFP marker in the genome of recombinant viruses were confirmed by PCR. Despite forming smaller plaques, the BoHV-1△gE recombinants replicated in MDBK cells with similar kinetics and to similar titers to that of the parental virus (SV56/90), demonstrating that the gE deletion had no deleterious effects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle pathogen; Immunization; Control; Differential vaccine.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900843

In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a)
Provedor de dados: 58
Autores: Spilki,Fernando R.; Franco,Ana C.; Rijsewijk,Frans A. M.; Weiblen,Rudi; Flores,Eduardo F.; Roehe,Paulo M.
This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis; Differential vaccine; In vitro growth characteristics; GE- recombinant; BHV-1.2a; Bovine herpesviruses.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200017

Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil
Provedor de dados: 58
Autores: Franco,Ana C.; Rijsewijk,Frans A.M.; Flores,Eduardo F.; Weiblen,Rudi; Roehe,Paulo M.
This paper describes the construction and characterization of a Brazilian strain of bovine herpesvirus type 1.2a (BoHV-1.2a) with a deletion of the glycoprotein E (gE) gene. The deletion was introduced by co-transfection of a deletion fragment containing the 5´and 3´gE flanking regions and genomic DNA of wild type BoHV-1 into bovine cells. Isolation of gE deletion mutant was performed by immunoperoxidase staining with an anti-gE monoclonal antibody. Viral clones were plaque purified and further examined by restriction endonuclesase digestion and Southern blot hybridization. This gE deletion mutant will be evaluated as a vaccinal virus, in order to determine its potential use for a differential vaccine.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis; BoHV-1; Deletion mutant; Differential vaccine.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300018