Consulta: subjectFacets:"Dot-ELISA"
Registros recuperados: 6
Data/hora: 02/06/2024 01:18:05

Standardization and validation of Dot-ELISA assay for Paracoccidioides brasiliensis antibody detection
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Kamikawa,Camila Mika; Mendes,Rinaldo Poncio; Vicentini,Adriana Pardini.
Abstract  Background  Paracoccidioidomycosis (PCM) is a neglected systemic mycosis caused by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. However, in some situations, access to the site of injury prevents the collection of biological material. A variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a certain degree of certainty, thus optimizing time. The aim of this study was to standardize and validate the Dot-ELISA (DE) assay, comparing it with the serological standard, double immunodiffusion (DI).  Methods  In order to standardize the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Immunodiagnostic tool; Dot-ELISA; Validation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100305

Detection of three Allexivirus species infecting garlic in Brazil
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Melo Filho,Péricles de Albuquerque; Nagata,Tatsuya; Dusi,André Nepomuceno; Buso,José Amauri; Torres,Antonio Carlos; Eiras,Marcelo; Resende,Renato de Oliveira.
Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium sativum; Virus detection; Coat protein; Dot-ELISA; RT-PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000800002

Diagnóstico biológico e molecular e análise da seqüência de nucleotídeos do gene da proteína capsidial de um isolado do Apple stem pitting virus
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: Radaelli,Paula; Nickel,Osmar; Schons,Jurema; Aragão,Francisco J.L.; Fajardo,Thor V. M.
O Apple stem pitting virus (ASPV) foi detectado por RT-PCR em amostras de cultivares de pereiras européias (Pyrus communis L.) cvs. Starkrimson e Abate Fetel, e asiáticas (P. pyrifolia var. culta) cvs. Kousui e Housui coletadas no início do outono de 2003 em pomar da Estação Experimental da Embrapa Uva e Vinho, Vacaria, RS. Utilizando várias combinações de oligonucleotídeos, foram amplificados fragmentos de DNA de 269 e 1554 pb, este último contendo o gene completo (1131 nt) da proteína capsidial do ASPV. Outro fragmento amplificado de 291 pb compreende parte do gene da polimerase viral. Estes fragmentos constituem-se em um excelente instrumento de diagnóstico do ASPV em pereiras. A comparação das seqüências de nucleotídeos do gene da proteína capsidial do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrus communis; P. pyrifolia; Malus spp.; Flexiviridae; ASPV; Dot-ELISA; Imunoblot; RT-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582006000100009

Comparison of dot-ELISA and standard ELISA for detection of Neisseria meningitidis outer membrane complex-specific antibodies
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Belo,Elza FT; Farhat,Calil K; Gaspari,Elizabeth N De.
Dot-ELISA using the outer membrane complex antigens of Neisseria meningitidis as a target was standardized for rapid detection of meningococcal-specific antibodies in human serum. We investigated the level of meningococcal-specific IgG, IgA, and IgM in serum using dot-ELISA with outer membrane antigens prepared from Neisseria meningitidis serotype B:4.19:P1.15,3,7,9 (a strain isolated from a Brazilian epidemic). The dot-ELISA is based on the same principles as the standard ELISA and is useful for detection of anti-N. meningitidis B antibodies in serum of patients with meningococcal infections. For the assay, outer membrane complexes (OMCs) were absorbed by nitrocellulose membrane and blocked with a 5% skim milk solution. Serum samples were drawn upon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neisseria meningitidis; Dot-ELISA; ELISA; Antibodies; Outer membrane complex.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100008

The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Barbosa,G.H.T.S.; Santana,E.M.; Almeida,A.M.P.; Araujo,A.M.; Fatibello-Filho,O.; Carvalho Jr.,L.B.
F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Filter paper; Polyvinyl alcohol; Glutaraldehyde; ELISA; Dot-ELISA; Plague.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013

Canine ehrlichiosis: clinical, hematological, serological and molecular aspects
Provedor de dados: Ciência Rural
Autores: Nakaghi,Andréa Cristina Higa; Machado,Rosangela Zacarias; Costa,Mirela Tinucci; André,Marcos Rogério; Baldani,Cristiane Divan.
The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and Immunofluorescent Antibody Test - IFAT), and demonstrate the most suitable test for the diagnosis of different stages of infection. Blood samples and clinical data were collected from 30 dogs examined at the Veterinary Teaching Hospital, UNESP, Jaboticabal, SP, Brazil. The clinical signs most frequently observed were apathy, anorexia, pale mucous membrane, fever, lymphadenopathy, splenomegaly, hemorrhages and uveitis. Evaluating the humoral immune response, 63.3% of the sera were IFAT positive, while 70% were Dot-ELISA positive. By nestedPCR 53.3% of the samples were positive. Comparing these techniques...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ehrlichia canis; Dog; IFAT; Dot-ELISA; Nested PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300027