Consulta: subjectFacets:"Electron microscopy"
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Data/hora: 06/06/2024 01:39:16

Determinación del agente causal de la pudrición del cogollo del Agave tequilana Weber var. Azul en el estado de Jalisco.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Cen Caamal, Julio César.
En los últimos años la superficie cultivada de Agave tequilana Weber var. Azul en el estado de Jalisco ha disminuido, debido a la presencia de una enfermedad cuyos síntomas se manifiestan como pudrición blanda del cogollo y muerte de la planta. El objetivo principal fue determinar el agente causal de esta enfermedad. Para ello se realizaron aislamientos, pruebas bioquímicas y moleculares mediante la amplificación del fragmento 16S DNAr y pruebas de patogenicidad con resultados positivos. El patógeno purificado fue una bacteria Gram positiva; en microscopia electrónica se observaron células en forma de bacilo, con presencia de endosporas en la parte terminal  y flagelos peritricos. Creció hasta 50°C en medio de agar nutritivo (AN). Este organismo, se...
Palavras-chave: Patogenicidad; Prueba molecular; Microscopia electrónica; Pathogenicity; Molecular testing; Electron microscopy; Fitopatología; Maestría.
Ano: 2012
URL: http://hdl.handle.net/10521/1747

Biosíntesis de lignina y comportamiento productivo en tres pastos de clima templado.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Vargas Romero, Juan Manuel.
Tres especies de gramíneas de clima templado, el híbrido Festulolium y sus progenitores Festuca arundinacea y Lolium perenne, se evaluaron en invernadero para determinar el crecimiento, fracciones de la fibra y características anatómicas de cada especie. Además se extrajo el ADN de las tres gramíneas para realizar un análisis molecular y calcular correlaciones entre los marcadores y las variables analizadas. De acuerdo con los resultados, el Festulolium fue distinto a F. arundinacea y L. perenne en el número de láminas por tallo; respecto a la abundancia relativa de lignina, área de xilema y floema, el contenido fue similar entre Festulolium y L. perenne, mientras que los valores de densidad de tricomas en el haz fueron semejantes entre Festulolium y F....
Palavras-chave: Paredes celulares; FDN; FDA; Estomas; Tricomas; Microscopía electrónica; Festulolium; Cell walls; NDF; FDN; Trichomes; Electron microscopy; Ganadería; Doctorado; Festuca arundinacea; Lolium perenne.
Ano: 2012
URL: http://hdl.handle.net/10521/744

Etude au microscope electronique a balayage des relations entre bacteries et matieres en suspension en milieu marin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cormier, M; Bassoulet, P; Pommepuy, Monique.
Near outfall areas numerous bacteria appear associated to colloidal organic matter present in littoral sea water. Arrangements into colonies are shown. Surface structures are observed which allow bacteria's attachment each to other and to organic matter.
Tipo: Text
Palavras-chave: Bacteria; Outfalls; Electron microscopy; Flocculation; Colloids; Adhesion; Suspended particulate matter.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-966.pdf

Response of a kleptoplastidic foraminifer to heterotrophic starvation: photosynthesis and lipid droplet biogenesis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Jauffrais, Thierry; Lekieffre, Charlotte; Schweizer, Magali; Jesus, Bruno; Metzger, Edouard; Geslin, Emmanuelle.
The aim of this work is to document the complex nutritional strategy developed by kleptoplastic intertidal foraminifera. We study the mixotrophic ability of a common intertidal foraminifer, E. williamsoni, by (1) investigating the phylogenetic identity of the foraminiferal kleptoplasts, (2) following their oxygenic photosynthetic capacity, and (3) observing the modification in cellular ultrastructural features in response to photoautotrophic conditions. This was achieved by coupling molecular phylogenetic analyses and TEM observations with non-destructive measurements of kleptoplast O2 production over a 15-day experimental study. Results show that the studied E. williamsoni actively selected kleptoplasts mainly from pennate diatoms and had the ability to...
Tipo: Text
Palavras-chave: Foraminifera; Electron microscopy; DNA barcoding; Kleptoplasty; Lipids.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59894/63074.pdf

Detection of Bonamia ostreae and B. exitiosa (Haplosporidia) in Ostrea edulis from the Adriatic Sea (Italy)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Nardsi, V.; Arzul, Isabelle; Cargini, D.; Mosca, F.; Calzetta, A.; Traversa, D.; Robert, Maeva; Joly, Jean-pierre; Chollet, Bruno; Renault, Tristan; Tiscar, P. G.
The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover,...
Tipo: Text
Palavras-chave: Bonamia spp.; Ostrea edulis; Molecular analysis; Electron microscopy.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11284/7825.pdf

Ultrastructural Effects of the Propineb on Brain of Fetuses During Rat Pregnancy
Provedor de dados: International Journal of Morphology
Autores: Ekinci,C; Tahaoglu,A. E; Yavuz,D; Deveci,E; Aktas,A; Yilmaz,T; Yumusak,Ö; Yükselmis,O.
Propineb is a fungicide with a propylene-bis-dithiocarbamate structure. Pregnant Wistar rats were exposed to 400 ppm propineb concentrations in 5 ml distilled water, 5 days per week until the end of pregnancy. The rats were treated with propineb for 16 days and the brains of litter rats were sacrificed at first day of birth after which their brains were collected. Ultrastructural examination of the brains of the fetuses and propineb-treated pregnant females revealed a variety of histopathological effects. We suggest that mitochondrial damage may be an effective factor for neuron necrosis. These results supported the proposal that the exposure to fungicides such as propineb and to other naturally occurring compounds which inhibit mitochondrial function, may...
Tipo: Journal article
Palavras-chave: Propineb; Electron microscopy; Neonatal rats; Brain.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400054

The Effects of Electromagnetic Fields on the Ultrastructure of Heart
Provedor de dados: International Journal of Morphology
Autores: Söker,Sevda; Sert,Cemil; Deniz,Mustafa; Tekmen,Is¸il; Akkus,Murat; Nergiz,Yusuf.
Increasingly the use and convenience of electrical appliances in our daily lives are the cause of harmful effects caused by electromagnetic fields (EMF). The aim of this study was to research the effect of EMF on the ultrastructure of the heart in EMF exposed rats. In this study 45 male Sprague Dawley rats ranging in weight between 260 and 280 grams were used. The rats were divided into 3 groups, control (n:15), Sham (n:15) and EMF group (n: 15) and exposed for 14 days 3 hours per day; gauss levels at 2.5 were applied to the EMF group, while the sham group in the same environment in Plexiglas cage was kept for 14 days 3 hours per day without magnetic field exposure. Control group at 14/10 hours light dark cycle fed in normal cages for 14 days. After two...
Tipo: Journal article
Palavras-chave: Electromagnetic field; Heart; Electron microscopy.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300050

An Electron Microscopic Study of the Antifertility Potential of Rosemary (Rosmarinus officinalis L.) in Male Albino Rats
Provedor de dados: International Journal of Morphology
Autores: El-Din,Rania A. Salah; El-Shahat,Abd El-Rahman; Elmansy,Rasha Ahmed.
The present work was aimed at studying the antifertility potential of the commonly used herb, rosemary in the male albino rats using electron microscopy as the method of investigation. Ethanolic extract of the rosmary prepared and administered orally in two different doses for a period of three months to the animals. At the end of the experiment animals were sacrificed and testes removed. Sections for the electrone microscopy prepared and changes were observed. The present results showed evident microscopic changes in the testis of the animals received higher dose of the drug. Most of the seminiferous tubules were compressed, having irregular basement membrane and devoid of any spermatogenic cells. The present work revealed a clear morphological evidence...
Tipo: Journal article
Palavras-chave: Antifertility potential; Rosemary; Rosmarinus officinalis L; Electron microscopy; Male albino rats.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200051

Micromorphology and systematic distribution of pit membrane thickenings in Oleaceae: Tori and pseudo-tori
Provedor de dados: Naturalis Digital Academic Repository
Autores: Rabaey, D.; Huysmans, S.; Lens, F.; Smets, E.; Jansen, S.
Recent studies on the functional significance of pit membranes in water conducting cells have renewed general interest in their micromorphology. At least two types of pit membrane thickenings have been described in angiosperm families, i.e. genuine tori and pseudo-tori. This study explores the distribution and morphology of pit membrane thickenings in 69 species and 23 genera within Oleaceae using light and electron microscopy. Torus-bearing pit membranes are confirmed for Osmanthus, and new records are reported for Chionanthus retusa, Picconia azorica, and P. excelsa, but not for the other species studied of Chionanthus. This infrageneric variation suggests that tori represent a plastic feature that has evolved more than once within the family as the...
Tipo: Article / Letter to the editor
Palavras-chave: Electron microscopy; Pit aperture; Pit membrane; Plasmodesmata; Pseudo-torus; Torus; Tracheid; Vessel element; 42.56.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/429536

Ultrastructural and functional aspects of the spermatheca in the american harlequin bug, Murgantia histrionica (Hemiptera: Pentatomidae)
Provedor de dados: Neotropical Entomology
Autores: Stacconi,MVR; Romani,R.
The spermatheca of Murgantia histrionica (Hahn) was investigated using fluorescence, scanning and transmission electron microscopy. The aim of the study was to elucidate the structure of this organ, pointing out differences between mated and unmated females. Results have shown an elaborated cuticular structure associated with muscular and glandular tissues. The spermatheca is joined with the common oviduct by the spermathecal duct, forming a thin saccular dilation through two consecutive invaginations. The distal part of the organ is formed by a series of two communicating cuticular chambers. The first cylindrical-shaped chamber, corresponding to the coiled region, is wrapped by longitudinal muscular fibers suspended between two cuticular flanges. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemiptera; Functional anatomy; Spermathecal gland; Electron microscopy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000200011

Morphometry and morphology of the antennae of Panstrongylus megistus Burmeister, Rhodnius neglectus Lent, Rhodnius prolixus Stal and Triatoma vitticeps Stal (Hemiptera: Reduviidae)
Provedor de dados: Neotropical Entomology
Autores: Rosa,João A da; Freitas,Silvia C M de; Malara,Flávio F; Rocha,Cláudia S.
The length of the four right antennal segments from nymphs and adults of Panstrongylus megistus Burmeister, Rhodnius neglectus Lent, Rhodnius prolixus Stal and Triatoma vitticeps Stal were measured. The length of the antennal segments of the adults of all four species, 4th and 5th instars of P. megistus, and 5th instar of R. neglectus and R. prolixus followed the same pattern: 2nd&gt;3rd&gt;4th&gt;1st. The pattern of 1st and 2nd instars of P. megistus was: 4th&gt;3rd&gt;2nd&gt;1st. For 3rd instars of P. megistus, 1st and 2nd instars of R. neglectus and R. prolixus they were: 3rd&gt;4th&gt;2nd&gt;1st. Third and 4th instars of Rhodnius neglectus and R. prolixus had a pattern of: 3rd&gt;2nd&gt;4th&gt;1st. Only T. vitticeps showed the same pattern...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triatominae; Mensuration; Electron microscopy; Sensilla.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000200011

Morphological analysis of the oviduct, oviducal gland and isthmus of the blue shark Prionace glauca (Linnaeus, 1758) (Elasmobranchii: Carcharhiniformes)
Provedor de dados: Neotropical Ichthyology
Autores: Rangel,Bianca S.; Bruno,Carlos E. Malavasi; Salmon,Thierry; Ciena,Adriano P.; Miglino,Maria A.; Amorim,Alberto F.; Rici,Rose E. G.
ABSTRACT Oviducal gland present in elasmobranchs is correlated to the organism's reproductive strategy, and its functions are to produce mucus, to form the egg's tertiary envelope and to store sperm. The gland contains four zones: club, papillary,bafﬂe and terminal. The structures of the oviduct, oviducal gland and isthmus of blue shark Prionace glauca were described using macroscopic, light microscopy and scanning electron microscopy techniques. The epithelium of the oviduct and isthmus is folded and is a simple, columnar, ciliated lining epithelium with glandular cells. In the oviducal gland, the lining tissues in the four zones are similar to the oviduct and isthmus lining. The terminal zone shows the presence of sperm in the lumen of the secretory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cartilaginous fish; Electron microscopy; Nidamental gland; Reproduction; Shell gland.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000400663

Structural and Molecular Differences in the Host Parasite Relationship between the Enteric (Coccidian) and Exoenteric Forms of Toxoplasma gondii
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Ferguson, D. J. P.
Palavras-chave: Toxoplasma gondii; Host/parasite relationship; Immunocytochemistry; Electron microscopy.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/328

Studies on the Morphology of Sarcocysts in Thoroughbred Horses in Japan
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Yamada, Masaji; Yukawa, Masayoshi; Sekikawa, Hiroho; Kenmotsu, Minoru; Mochizuki, Koshi.
Palavras-chave: Thoroughbred; Sarcocystis; Cyst wall; Electron microscopy.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/179

Scanning electron microscope studies on some species of the centric diatom genera &lt;i&gt;Thalassiosira&lt;/i&gt; and &lt;i&gt;Coscinodiscus&lt;/i&gt;
Provedor de dados: Open Marine Archive
Autores: Somers, D.
By means of a scanning electron microscope, the submicroscopic structure of four &lt;i&gt;Thalassiosira&lt;/i&gt; species and two &lt;i&gt;Coscinodiscus&lt;/i&gt; species is studied. Five of these taxa are more or less similar in the arrangement of the areoles; the sixth one is aberrant. Besides the description of each species and the description of the submicroscopic structure of the auxospore of &lt;i&gt;Thalassiosira nordenskiöldii&lt;/i&gt;, a classification is given of the six taxa studied.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diatoms; Electron microscopy; Microscopy; Coscinodiscus; Thalassiosira.
Ano: 1972
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3457

Histomorphological changes by epididymal lithiasis in roosters
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Geraldo,I; Mahecha,GAB; Martins,NRS.
Epididymal lithiasis (EL) histopathology is described using light and electronic microscopy in roosters (Gallus gallus domesticus) naturally affected by EL in Minas Gerais, Brazil. The histologic and morphological changes by EL in roosters was performed regarding cellular and subcellular details through light and electron microscopy. Efferent ductules epithelium lysosomal increase in size and numbers, membrane rupture, cellular vacuolation, ciliary loss, basal membrane degeneration, inflammatory reaction with mononuclear infiltrations, edema, epithelial and vascular endothelium losses were described. All industrial and freerange chickens showed EL in varying degrees in the efferent ductules (ED). However, ED altered areas did not correlate with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epididymal lithiasis; Histopathology; Light microscopy; Electron microscopy; Gallus gallus domesticus; Infertility; Rooster; Testicular calculus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100004

Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Vicentin,Rayssa Pereira; Santos,Jessé Valentim dos; Labory,Cláudia Regina Gontijo; Costa,Amanda Monique da; Moreira,Fatima Maria de Souza; Alves,Eduardo.
ABSTRACT Preliminary results of in vitro experiments with multicontaminated soils and solid media indicated that nodulating diazotrophic bacteria of the genus Cupriavidus are promising for the remediation of contaminated environments due to their symbiosis with legumes and metal tolerance. Thus, strains of Cupriavidus spp. (LMG 19424T, UFLA 01-659, UFLA 01-663, and UFLA 02-71) were tested for their ability to tolerate and bioaccumulate cadmium (Cd), copper (Cu), and zinc (Zn) in Luria-Bertani broth. Changes in the growth pattern of Cupriavidus strains in the presence or absence of heavy metals were analyzed by scanning electron microscopy and metal allocation by transmission electron microscopy, to clarify the mechanisms of bioremediation. Highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metals; Electron microscopy; Diazotrophic bacteria; Bioremediation; Tolerance mechanisms.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832018000100407

Nanoparticles and nanostructure morphology of a Red Latosol in rehabilitation
Provedor de dados: Revista Brasileira de Engenharia Agrícola e Ambiental
Autores: Souto Filho,Sebastião N.; Alves,Marlene C.; Monreal,Carlos M.; Bonini,Carolina dos S. B.
ABSTRACT In the process of rehabilitation of a soil, two points are fundamental: to define optimal interferences to accelerate the rehabilitation process and the most appropriate indicators to diagnose its quality. Therefore, this study aimed to investigate the nanoparticle and nanostructure morphology of a Red Latosol in rehabilitation for eight years. The soil under rehabilitation process was compared with its natural state and degraded. In the topsoil, nanoparticles (ø &lt; 100 nm) and fine clay (ø &lt; 200 nm) were quantified and the nanostructures morphology was studied using images obtained by transmission electron microscopy. Soil porosity, bulk density and carbon, nitrogen and hydrogen contents were analyzed. It was found that the nanoparticles and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Degraded soil; Sewage sludge; Electron microscopy; Soil porosity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662017000800530

Rostrum length, mandible serration, and food and salivary canals areas of selected species of stink bugs (Heteroptera, Pentatomidae)
Provedor de dados: Revista Brasileira de Entomologia
Autores: Depieri,Rogério A.; Panizzi,Antônio R.
Laboratory studies were conducted to compare rostrum length morphology of mandible serration and area of food and salivary canals of Dichelops melacanthus (Dallas) (Dm), Euschistus heros (F.) (Eh), Nezara viridula (L.) (Nv), and Piezodorus guildinii (Westwood) (Pg) (Heteroptera: Pentatomidae). Nv showed the longest (5.9 mm) and Pg the shortest (3.5 mm) rostrum length; Dm and Eh were intermediate. Length and width of mandible tip areas holding serration was bigger for Nv (106.0 and 30.2 µm, respectively) and smaller for Pg (71.1 and 23.7 µm), with all species having four central teeth and three pairs of lateral teeth. The inner mandible surface showed squamous texture. Cross-section of food and salivary canals (Fc and Sc) indicated greater area for Nv and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electron microscopy; Mouth parts morphology; Teneral adults.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262010000400008

Detection of viral particles in feces of young dogs and their relationship with clinical signs
Provedor de dados: Revista de Microbiologia
Autores: Amo,Alicia N. del; Aprea,Adriana N.; Petruccelli,Miguel A.
Gross and light microscopic studies of 100 stool specimens of young dogs were carried out. Viral particles were detected in 31% of the analized samples using negative contrast electron microscopic diagnostic technique. Parvo-like virus, corona-like virus and other non-identified particles were observed in 17%, 7% and 2% of the samples, respectively. Parvo-like and corona-like viruses were found together in 5% of the samples. More than half (58.82%) of the positive parvo-like virus specimens were from dogs aged between 6 weeks and 6 months. 42.85% of the corona-like virus positive samples were detected in dogs between 6 weeks and 6 months and a similar percentage was found in dogs older than six months of age. Dual infections with parvo-like and corona-like...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parvo-like virus; Corona-like virus; Diarrhea; Dogs; Electron microscopy.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300009

MORPHOLOGICAL, CYTOLOGICAL, AND CULTURAL ASPECTS OF CURVULARIA PALLESCENS
Provedor de dados: Revista de Microbiologia
Autores: Freire,Sônia Valéria Pereira; Paiva,Laura Mesquita; Lima,Elza Aúrea de Luna-Alves; Maia,Leonor Costa.
Curvularia pallescens Boedijn (Hyphomycetes) is redescribed with the aid of a scanning electron microscope, and the optimal cultural conditions for growing this fungus are discussed. Cytological analysis and nuclear condition, observed through the HCl-Giemsa technique, showed vegetative and reproductive structures (hypha and conidia) formed by uni, bi, tri, and multinucleated segments. Cultures of C. pallescens in Complete medium and in Potato dextrose agar varied on growth, on aspects of the border of the colonies and also on medium pigmentation. The Complete medium and the temperature between 25-28°C were the most indicated for growth of C. pallescens.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conidial development; Fungi; Electron microscopy; Hyphomycetes.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300010

Boron affects the growth and ultrastructure of castor bean plants
Provedor de dados: Scientia Agricola
Autores: Silva,Denis Herisson da; Rossi,Monica Lanzoni; Boaretto,Antonio Enedi; Nogueira,Neusa de Lima; Muraoka,Takashi.
The cultivation of oleaginous plants like the castor bean guarantees employment for agricultural families and can contribute in energy and chemical sectors, especially in the northeastern semi-arid regions of Brazil. Boron (B) deficiency is a widespread nutritional disorder despite the fact that various anthropogenic sources with high B content may increase soil B to toxic levels for plants. The present study was designed to investigate the ultrastructural effects of boron deficiency and toxicity on castor bean plants which were grown under greenhouse condition using plastic containers with 10 L of nutrient solution. Boron treatments comprised: control (no B); 0.27 mg L-1, 5.40 mg L-1 B pots (one plant per pot), tested in a completely randomized design...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electron microscopy; Middle lamellae; Micronutrient; Xylem; Starch.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000600014

Silicon and rice disease management
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: Rodrigues,Fabrício A; Datnoff,Lawrence E.
The element silicon (Si) is not considered an essential nutrient for plant function. Nevertheless, Si is absorbed from soil in large amounts that are several fold higher than those of other essential macronutrients in certain plant species. Its beneficial effects have been reported in various situations, especially under biotic and abiotic stress conditions. The most significant effect of Si on plants, besides improving their fitness in nature and increasing agricultural productivity, is the restriction of parasitism. There has been a considerable amount of research showing the positive effect of Si in controlling diseases in important crops. Rice (Oryza sativa), in particular, is affected by the presence of Si, with diseases such as blast, brown spot and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mechanisms of host resistance; Electron microscopy; PR-genes; Phenolic-like compounds; Phytoalexins.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000500001

Cytopathological characterization of Mal de Río Cuarto virus in corn, wheat and barley
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: ARNEODO,JOEL D.; LORENZO,EUGENIA; LAGUNA,IRMA G.; ABDALA,GUILLERMINA; TRUOL,GRACIELA A.
The Mal de Río Cuarto disease is caused by Mal de Río Cuarto virus (MRCV) transmitted by Delphacodes kuscheli. Comparative studies were carried out on the cytopathological alterations produced by MRCV in corn (Zea mays), wheat (Triticum aestivum) and barley (Hordeum vulgare), as seen with a transmission electron microscope. Corn plants were infected with viruliferous D. kuscheli collected from the endemic disease area (i.e. Río Cuarto County, Córdoba, Argentina). For the viral transmission to small grain cereal plants, laboratory rared insects were used. In this case, the inoculum source was wheat and barley plants infected with MRCV isolate grown in a greenhouse. Leaf samples with conspicuous symptoms were collected: enations and size reduction in corn;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Virus characterization; Electron microscopy; Fijivirus; Reoviridae; Cereals.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300010

Detection and coat protein gene characterization of an isolate of Grapevine virus B from corky bark-affected grapevines in Southern Brazil
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: NICKEL,OSMAR; FAJARDO,THOR V. M.; ARAGÃO,FRANCISCO J. L.; CHAGAS,CÉSAR M.; KUHN,GILMAR B.
An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GVB; Vitis spp.; Vitivirus; Electron microscopy; ELISA; Western blot; GVA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300007

Coat protein and RNAs of Cole latent virus are not present within chloroplasts of Chenopodium quinoa-infected cells
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: Belintani,Priscila; Gaspar,José O.
Cole latent virus (CoLV), genus Carlavirus, was studied by electron microscopy and biochemical approaches with respect both to the ultrastructure of the Chenopodium quinoa infected cells and to its association with chloroplasts. The CoLV was observed to be present as scattered particles interspersed with membranous vesicles and ribosomes or as dense masses of virus particles. These virus particles reacted by immunolabelling with a polyclonal antibody to CoLV. Morphologically, chloroplasts, mitochondria and nuclei appeared to be unaltered by virus infection and virus particles were not detected in these organelles. However, virus particle aggregates were frequently associated with the outer membrane of chloroplasts and occasionally with peroxisomes....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassica; Electron microscopy; Carlavirus; Virus replication; Plant virus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000100012

Nutritious tissue in petals of Annonaceae and its function in pollination by scarab beetles
Provedor de dados: Acta Botanica Brasilica
Autores: Gottsberger,Gerhard; Webber,Antonio Carlos.
ABSTRACT The feeding of pollinating dynastid-scarab beetles on nutritious tissue of Annonaceae flowers results in macroscopically visible gnawing marks on petals. In the present paper, we present and discuss examples of such gnawing marks on Annonaceae from the Cerrado and the Amazon Forest in Brazil. The localization of gnawing marks on the petals and the histochemistry of the nutritious tissues are emphasized. In some species, nutritious tissue is apparently distributed among all petals, while in other species it is more or less diffusely localized. There are also cases in which nutritious tissue occurs only on clearly localized regions of the inner petals. Petals of selected Amazon species were stained, and studied by light and scanning electron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado and Amazon forest Annonaceae; Electron microscopy; Histochemistry; Lipids; Mucilage cells in petals; Starch; Tannins.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000200279

Interconnection between microstructure and microhardness of directionally solidified binary Al-6wt.%Cu and multicomponent Al-6wt.%Cu-8wt.%Si alloys
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: VASCONCELOS,ANGELA J.; KIKUCHI,RAFAEL H.; BARROS,ANDRÉ S.; COSTA,THIAGO A.; DIAS,MARCELINO; MOREIRA,ANTONIO L.; SILVA,ADRINA P.; ROCHA,OTÁVIO L.
An experimental study has been carried out to evaluate the microstructural and microhardness evolution on the directionally solidified binary Al-Cu and multicomponent Al-Cu-Si alloys and the influence of Si alloying. For this purpose specimens of Al-6wt.%Cu and Al-6wt.%Cu-8wt.%Si alloys were prepared and directionally solidified under transient conditions of heat extraction. A water-cooled horizontal directional solidification device was applied. A comprehensive characterization is performed including experimental dendrite tip growth rates (VL) and cooling rates (TR) by measuring Vickers microhardness (HV), optical microscopy and scanning electron microscopy with microanalysis performed by energy dispersive spectrometry (SEM-EDS). The results show, for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solidification; Metals and alloys; Electron microscopy; Hardness.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000301099

Dermocystidium sp. in the gills of farmed Oreochromis niloticus in Brazil
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: STECKERT,LILIAN D.; CARDOSO,LUCAS; TANCREDO,KAREN R.; MARTINS,MAURÍCIO L.; JERÔNIMO,GABRIELA T.
Abstract: The genus Dermocystidium is very comprehensive in the host and site of infection, however this is the first report of the occurrence of Dermocystidium sp. in the gills of Nile tilapia. This study was carried out in a fish farming located in the state of Santa Catarina, Brazil. No mortalities were reported in the facility studied and the animals were clinically healthy. During the histopathological analysis of the gills, 8.33% of the fish presented spores of Dermocystidium sp. in the gill tissue. The spores reported herein had a mean length and width of 6.206 x 5.233 μm and a refractile body diameter of 1.965 μm and were studied by histopathology and Transmission Electron Microscopy. This study highlights the importance of a new branchial pathogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish farming; Dermatocystidiosis; Nile tilapia; Histology; Electron microscopy; Spores.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500865

Correlative microscopy of Purkinje cells
Provedor de dados: Biocell
Autores: Castejón,Orlando J.
The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebellar cells; Synaptic contacts; Electron microscopy; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100001

Natural infection of Viola cornuta (Violaceae) with Cucumber mosaic virus, subgroup I
Provedor de dados: Biocell
Autores: Arneodo,Joel; de Breuil,Soledad; Lenardon,Sergio; Conci,Luis.
Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Viola cornuta; Cucumber mosaic virus; Electron microscopy; Serology; AC-RT-PCR.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200008

Sperm ultrastructure of Mytella (Bivalvia) populations from distinct habitats along the northern coast of São Paulo State, Brazil
Provedor de dados: Biocell
Autores: Orlandi Introíni,Gisele; Martins Maester,Fernanda; Pedini Pereira Leit,Fosca; Recco-Pimentel,Shirlei Maria.
Ultrastructural analyses of bivalve spermatozoa are relevant in studies that aim to identify taxonomic traits for the purposes of discriminating species and conducting phylogenetic studies. In the present work, spermatozoa of mussel specimens of the genus Mytella, collected from two populations living in distinct habitats, were examined by electron microscopy. The objective was to identify sperm ultrastructural taxonomic traits that could be used to differentiate Mytella species. The specimens were from populations that live in intertidal zones on the southeast coast of Brazil, either buried in muddy-sand sediment or anchored to rocky substrates. The acrosomal vesicle was conical and long, the axial rod extended from the nucleus to the acrosome, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mytilidae; Spermatozoa; Electron microscopy; Mussel.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300002

Extracellular breakdown of collagen by mice decidual cells. A cytochemical and ultrastructural study
Provedor de dados: Biocell
Autores: Godosevicius Katz,Sima.
The interaction of antimesometrial decidual cells and collagen fibrils was studied by light microscopy and ultrastructural cytochemistry in fed and acutely fasted mice on days 9-11 of pregnancy. Fibrillar elements in the extracellular space consisted of collagen fibrils and filamentous aggregates (disintegrating collagen fibrils). Intracellular vacuoles exhibited typical collagen immersed in electron-translucent material (clear vacuoles) and faint cross-banded collagen immersed in electron-opaque material (dark vacuoles). Fibrillar elements showed extracellular acid phosphatase activity which was stronger in the region of mature decidua than in predecidual cells region in all animals; it was conspicuous in mature decidua of fasted animals. Intracellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decidua; Acid phosphatase; Fasting; Electron microscopy; Mice.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300003

Ultrastructural changes of the olfactory bulb in manganese-treated mice
Provedor de dados: Biocell
Autores: Villalobos,Virginia; Bonilla,Ernesto; Castellano,Alan; Novo,Ernesto; Caspersen,Ralph; Giraldoth,Debora; Medina-Leendertz,Shirley.
The effect of manganese toxicity on the ultrastructure of the olfactory bulb was evaluated. Male albino mice were injected intraperitoneally with MnCl2 (5 mg/Kg/day) five days per week during nine weeks. The control group received NaCl (0.9%). The olfactory bulbs of five mice from each group were processed for transmission electron microscopy after 2, 4, 6 and 9 weeks of manganese treatment. On week 2, some disorganization of the myelin sheaths was observed. After 4 weeks, degenerated neurons with dilated cisternae of rough endoplasmic reticulum and swollen mitochondria appeared. A certain degree of gliosis with a predominance of astrocytes with swollen mitochondria, disorganization of the endomembrane system, dilation of the perinuclear cisternae and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metal toxicity; Toxicity; Neurodegeneration; Electron microscopy.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300007

Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)
Provedor de dados: Biological Research
Autores: Leonova,Olga G; Karajan,Bella P; Ivlev,Yuri F; Ivanova,Julia L; Skarlato,Sergei O; Popenko,Vladimir I.
We have earlier shown that the typical Didinium nasutum nucleolus is a complex convoluted branched domain, comprising a dense fibrillar component located at the periphery of the nucleolus and a granular component located in the central part. Here our main interest was to study quantitatively the spatial distribution of nucleolar chromatin structures in these convoluted nucleoli. There are no "classical" fibrillar centers in D.nasutum nucleoli. The spatial distribution of nucleolar chromatin bodies, which play the role of nucleolar organizers in the macronucleus of D.nasutum, was studied using 3D reconstructions based on serial ultrathin sections. The relative number of nucleolar chromatin bodies was determined in macronuclei of recently fed, starved...
Tipo: Journal article
Palavras-chave: 3D reconstruction; Ciliates; Electron microscopy; Macronucleus; Nucleoli.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100010

Modeling pulmonary fibrosis by abnormal expression of telomerase/apoptosis/collagen V in experimental usual interstitial pneumonia
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Parra,E.R.; Pincelli,M.S.; Teodoro,W.R.; Velosa,A.P.P.; Martins,V.; Rangel,M.P.; Barbas-Filho,J.V.; Capelozzi,V.L.
Limitations on tissue proliferation capacity determined by telomerase/apoptosis balance have been implicated in pathogenesis of idiopathic pulmonary fibrosis. In addition, collagen V shows promise as an inductor of apoptosis. We evaluated the quantitative relationship between the telomerase/apoptosis index, collagen V synthesis, and epithelial/fibroblast replication in mice exposed to butylated hydroxytoluene (BHT) at high oxygen concentration. Two groups of mice were analyzed: 20 mice received BHT, and 10 control mice received corn oil. Telomerase expression, apoptosis, collagen I, III, and V fibers, and hydroxyproline were evaluated by immunohistochemistry, in situ detection of apoptosis, electron microscopy, immunofluorescence, and histomorphometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BHT experimental model; Usual interstitial pneumonia; Alveolar epithelial cell apoptosis; Telomerase activity; Collagen V; Electron microscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700567

Ultrastructural study of the neovagina following the utilization of human amniotic membrane for treatment of congenital absence of the vagina
Provedor de dados: Brazilian Journal of Medical and Biological Research
We present an ultrastructural study of the utilization of human amniotic membrane in the treatment of congenital absence of the vagina in 10 patients. All patients were surgically treated with application of an amniotic membrane graft using the modified McIndoe and Bannister technique. Sixty days after surgery, samples of the vaginal neo-epithelium were collected for transmission electron microscopy analysis. The ultrastructural findings consisted of a lining of mature squamous epithelium indicating the occurrence of metaplasia of the amniotic epithelium into the vaginal epithelium. The cells were arranged in layers as in the normal vaginal epithelium, i.e., superficial, intermediate and deep layers. There were desmosomes and cytoplasmic intermediate...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Amniotic membrane; Amnion; Electron microscopy; Congenital absence of vagina; Neovagina.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700007

Diversity among satellite glial cells in dorsal root ganglia of the rat
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nascimento,R.S.; Santiago,M.F.; Marques,S.A.; Allodi,S.; Martinez,A.M.B.
Peripheral glial cells consist of satellite, enteric glial, and Schwann cells. In dorsal root ganglia, besides pseudo-unipolar neurons, myelinated and nonmyelinated fibers, macrophages, and fibroblasts, satellite cells also constitute the resident components. Information on satellite cells is not abundant; however, they appear to provide mechanical and metabolic support for neurons by forming an envelope surrounding their cell bodies. Although there is a heterogeneous population of neurons in the dorsal root ganglia, satellite cells have been described to be a homogeneous group of perineuronal cells. Our objective was to characterize the ultrastructure, immunohistochemistry, and histochemistry of the satellite cells of the dorsal root ganglia of 17 adult...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Satellite glial cells; Peripheral nervous system; Vimentin; S-100; Electron microscopy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100011

Radioautographology: the proposal of a new concept
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nagata,T.
A new concept termed "radioautographology" is advocated. This term was synthesized from "radioautography" and "ology", expressing a new science derived from radioautography. The concept of radioautographology (RAGology) is that of a science whose objective is to localize radioactive substances in the biological structure of objects and to analyze and study the significance of these substances in the biological structure. On the other hand, the old term radioautography (RAG) is the technique used to demonstrate the pattern of localization of various radiolabeled compounds in specimens. The specimens used in biology and medicine are cells and tissues. They are fixed, sectioned and placed in contact with the radioautographic emulsions, which are exposed and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Light microscopy; Electron microscopy; Radioautography; Technique; Application.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800001

Effect of melatonin and time of administration on irradiation-induced damage to rat testes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Take,G.; Erdogan,D.; Helvacioglu,F.; Göktas,G.; Ozbey,G.; Uluoglu,C.; Yücel,B.; Guney,Y.; Hicsonmez,A.; Ozkan,S.
The effect of ionizing irradiation on testes and the protective effects of melatonin were investigated by immunohistochemical and electron microscopic methods. Eighty-two adult male Wistar rats were divided into 10 groups. The rats in the irradiated groups were exposed to a sublethal irradiation dose of 8 Gy, either to the total body or abdominopelvic region using a 60Co source at a focus of 80 cm away from the skin in the morning or evening together with vehicle (20% ethanol) or melatonin administered 24 h before (10 mg/kg), immediately before (20 mg/kg) and 24 h after irradiation (10 mg/kg), all ip. Caspace-3 immunoreactivity was increased in the irradiated group compared to control (P &lt; 0.05). Melatonin-treated groups showed less apoptosis as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Testes; Gamma-irradiation; Melatonin; Immunohistochemistry; Electron microscopy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700006

Centromere domain organization and histone modifications
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bjerling,P.; Ekwall,K.
Centromere function requires the proper coordination of several subfunctions, such as kinetochore assembly, sister chromatid cohesion, binding of kinetochore microtubules, orientation of sister kinetochores to opposite spindle poles, and their movement towards the spindle poles. Centromere structure appears to be organized in different, separable domains in order to accomplish these functions. Despite the conserved nature of centromere functions, the molecular genetic definition of the DNA sequences that form a centromere in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, in the fruit fly Drosophila melanogaster, and in humans has revealed little conservation at the level of centromere DNA sequences. Also at the protein level few...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Centromere; Kinetochore; Histone acetylation; Electron microscopy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500001

Electron microscopy of glial cells of the central nervous system in the crab Ucides cordatus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Allodi,S.; Taffarel,M.
Invertebrate glial cells show a variety of morphologies depending on species and location. They have been classified according to relatively general morphological or functional criteria and also to their location. The present study was carried out to characterize the organization of glial cells and their processes in the zona fasciculata and in the protocerebral tract of the crab Ucides cordatus. We performed routine and cytochemical procedures for electron microscopy analysis. Semithin sections were observed at the light microscope. The Thiéry procedure indicated the presence of carbohydrates, particularly glycogen, in tissue and in cells. To better visualize the axonal ensheathment at the ultrastructural level, we employed a method to enhance the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glial cells; Crustaceans; Electron microscopy; Cytochemistry; Ucides cordatus.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300013

An improved purification procedure for Leishmania RNA virus (LRV)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Souza,Marcos Michel de; Manzine,Livia Regina; Silva,Marcos Vinicius G. da; Bettini,Jefferson; Portugal,Rodrigo Vilares; Cruz,Angela Kaysel; Arruda,Eurico; Thiemann,Otavio Henrique.
Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania guyanensis; Leishmania RNA virus; LRV; Virus isolation; Electron microscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200044

Morphological and cytochemical characterization of spores and gills of Lepista sordida (Fungi: Basidiomycota)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Graf,Letícia V.; Schadeck,Ruth J. G.; Donatti,Lucélia; Buchi,Dorly F.
Some ultrastructural and cytochemical aspects of Lepista sordida are described. Basidiospores with verruculose and irregular ornamentations were observed through scanning electron microscopy. Confocal microscopy indicated that most of them exhibited a single large lipid body, no acidic vesicles and nucleus localized in the cell periphery.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basidiospores; Lipid body; Confocal microscopy; Electron microscopy; Lepista sordida.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300035

Simple homemade apparatus for harvesting uncultured magnetotactic microorganisms
Provedor de dados: Brazilian Journal of Microbiology
Autores: Lins,Ulysses; Freitas,Flavia; Keim,Carolina Neumann; Barros,Henrique Lins de; Esquivel,Darci Motta S.; Farina,Marcos.
A simple apparatus for harvesting uncultured magnetotactic microorganisms is described. This apparatus consists of a glass container with two openings. A large opening on the topside is used to introduce the sediment and water. The sediment and water are previously stored in loosely capped bottles previously tested for the presence of magnetotactic bacteria. The apparatus is exposed to a properly aligned magnetic field of a homemade coil and the bacteria are removed through the capillary end of the second opening of the container. Harvested bacteria can then be used to ultrastrucutral studies using electron spectroscopic imaging. Large numbers of magnetotactic bacteria consisting of cocci and rod-shaped cells were efficiently collected from different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnetotactic bacteria; Harvesting uncultured bacteria; Electron microscopy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200004

Antifungal activity of the ethanolic extracts of Punica granatum L. and evaluation of the morphological and structural modifications of its compounds upon the cells of Candida spp.
Provedor de dados: Brazilian Journal of Microbiology
Autores: Anibal,Paula Cristina; Peixoto,Iza Teixeira Alves; Foglio,Mary Ann; Höfling,José Francisco.
Ethanolic crude extracts prepared from the arils and seeds, pericarp, peels and from the whole fruit of Punica granatum, known as pomegranate, had their antifungal activity tested against Candida spp. The ethanolic crude extracts were analyzed by Mass Spectrometry and yielded many compounds such as punicalagin and galladydilacton. The extracts from the pericarp and peel showed activity against Candida spp., with MICs of 125 µg/mL. The effect of pericarp and peel extracts upon the morphological and structure of C. albicans and C. krusei were examined by scanning and transmission electron microscopy, with the visualization of an irregular membrane and hyphae, formation of vacuoles and thickening of the cell wall. The data obtained revealed potential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Punica granatum; Punicalagin; Antifungal activity; Candida spp.; Electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300027

Evaluation of antibacterial properties of Barium Zirconate Titanate (BZT) nanoparticle
Provedor de dados: Brazilian Journal of Microbiology
Autores: Mohseni,Simin; Aghayan,Mahdi; Ghorani-Azam,Adel; Behdani,Mohammad; Asoodeh,Ahmad.
So far, the antibacterial activity of some organic and inorganic compounds has been studied. Barium zirconate titanate [Ba(Zr xTi1-x)O3] (x = 0.05) nanoparticle is an example of inorganic materials. In vitro studies have provided evidence for the antibacterial activity of this nanoparticle. In the current study, the nano-powder was synthesized by sol-gel method. X-ray diffraction showed that the powder was single-phase and had a perovskite structure at the calcination temperature of 1000 ºC. Antibacterial activity of the desired nanoparticle was assessed on two gram-positive (Staphylococcus aureus PTCC1431 and Micrococcus luteus PTCC1625) and two gram-negative (Escherichia coli HP101BA 7601c and clinically isolated Klebsiella pneumoniae) bacteria according...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles; Antibiotics; Barium zirconate titanate; Ceramics; Electron microscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400033

Morphological characteristics and cell viability of coffee plants calli
Provedor de dados: Ciência Rural
Autores: Pádua,Marlúcia Souza; Paiva,Luciano Vilela; Silva,Luciano Coutinho da; Livramento,Kalynka Gabriella do; Alves,Eduardo; Castro,Ana Hortência Fonseca.
The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Electron microscopy; Tetrazolium test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400014

Essential oils on the control of stem and ear rot in maize
Provedor de dados: Ciência Rural
Autores: Teixeira,Glauco Antonio; Alves,Eduardo; Amaral,Douglas Carvalho; Machado,José da Cruz; Perina,Fabiano José.
Stem and ear rot caused by Stenocarpella maydis are responsible for severe losses in maize production. Treatment of seeds with fungicides may induce environmental damage. Hence, this study aimed to evaluate the effects of essential oils extracted from Cymbopogon winterianus, Thymus vulgaris, Cymbopogon citratus, Corymbia citriodora, Cinnamomum zeylanicum, and Syzygium aromaticum on the development of in vitro S. maydis. In addition, maize seeds were treated with these essential oils to determine their possible mode of action and effects. The oils from S. aromaticum, C. zeylanicum, and T. vulgaris inhibited fungal development at concentrations higher than 0.025%. The oils from S. aromaticum and C. zeylanicum showed seed germination rates of 89.0% and 84.5%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stenocarpella maydis; Zea mays; Alternative control; Electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001100004

Physical and functional evaluation of extruded flours obtained from different rice genotypes
Provedor de dados: Ciência e Agrotecnologia
Autores: Becker,Fernanda Salamoni; Eifert,Eduardo da Costa; Soares Junior,Manoel Soares; Tavares,July-Ana Souza; Carvalho,Ana Vânia.
The transformation of broken grains into native flours modified by extrusion is an alternative to add value to these co-products from the paddy rice processing. This study aimed to analyze the process of extrusion cooking on the physical and functional characteristics of extruded flours obtained from different rice genotypes (IRGA-417, BRS-Primavera and CNA-8502). The experimental design was completely randomized (3x2 factorial) with four original replicates and analysis of variance to assess particle size, instrumental color parameters (L*, a* and b*), water absorption index (WAI), milk absorption index (MAI), oil absorption index (OAI), water solubility index (WSI) and milk solubility index (MSI) of rice flour. The extrusion process promoted changes in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orysa sativa L; Co-products; Electron microscopy; Color; Solubility in water.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000400007