Consulta: subjectFacets:"Enterocytes"
Registros recuperados: 2
Data/hora: 02/06/2024 10:49:50

Immunohistochemical Studies on Duodenum, Spleen and Liver in Mice: Distribution of Ferroportin and Prohepcidin in an Inflammation Model
Provedor de dados: 77
Autores: D´Anna,María Cecilia; Giorgi,Gisela; Roque,Marta Elena.
Duodenum, spleen and liver have a crucial role in iron balance on the whole organism and are the major sites of Ferroportin (FPN) expression. Specific regulations between FPN and hepcidin are responsible for changes seen in physiopathological conditions such as inflammation. We studied in vivo effects of turpentine oil-induced acute inflammation on FPN expression, and its relation with prohepcidin and iron mobilization. Immunohistochemical procedures were performed using rabbit anti-mouse FPN and prohepcidin antibodies with goat-labeled polymer-HRP anti-rabbit (DAB) as secondary antibody. Plasma and tissular iron were also studied. Our results showed a notable expression and redistribution of duodenal FPN to basolateral membrane in turpentine-treated mice,...
Tipo: Journal article
Palavras-chave: Iron; Mobilization; Macrophages; Enterocytes.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300014

Cholesterol changes the fatty acid composition of rat enterocytes
Provedor de dados: 56
Autores: Gazzola,J.; Martins,E.F.; Miyasaka,C.K.; Palanch,A.C.; Vecchia,M.G.; Curi,R.
The effect of free cholesterol on the fatty acid composition and growth of rat fetal enterocytes was investigated in the absence and presence of 10% (v/v) fetal calf serum. Cholesterol caused a significant reduction of cell number after 6 and 12 h in culture. The fatty acid composition of enterocytes cultured in the presence of serum was also changed by the presence of 20 µM cholesterol. The fatty acid profile was determined by HPLC using fluorescence detection (325 nm excitation and 395 nm emission). Cholesterol (20 µM) increased the proportion (given in percentage of the total fatty acids) of the following fatty acids in cultured cells: lauric (by 42%), oleic (by 34%), linoleic (by 44%) and gamma-linolenic (by 20%) acids and reduced the proportion of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterocytes; Cholesterol; Fatty acid composition; Cell proliferation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100019