Consulta: subjectFacets:"Entomopathogen"
Registros recuperados: 15
Data/hora: 09/06/2024 04:46:46

Differential expression of the pr1A gene in Metarhizium anisopliae and Metarhizium acridum across different culture conditions and during pathogenesis
Provedor de dados: 74
Autores: Leão,Mariele Porto Carneiro; Tiago,Patricia Vieira; Andreote,Fernando Dini; de Araújo,Welington Luiz; de Oliveira,Neiva Tinti.
The entomopathogenic fungi of the genus Metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. The identification and characterization of these proteases can provide insight into how the fungus is capable of infecting a wide variety of insects and adapt to different substrates. In addition, the pr1A gene has been used for the genetic improvement of strains used in pest control. In this study we used quantitative RT-PCR to assess the relative expression levels of the pr1A gene in M. anisopliae and M. acridum during growth in different culture conditions and during infection of the sugar cane borer,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogen; Diatraea saccharalis; Quantitative RT-PCR; Expression pattern.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100086

Natural Enemies and Competitors of Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae) in Colombia
Provedor de dados: 82
Autores: BUSTILLO,ALEX E.; CÁRDENAS,REINALDO; POSADA,FRANCISCO J.
The objective of this research was to survey and record the natural enemies and competitors of the Coffee Berry Borer (CBB) Hypothenemus hampei (Ferrari) in the Colombian coffee region. Despite the recently introduction of CBB to Colombia, 25 natural enemies were recorded, including Beauveria bassiana. Except for this fungus, the other organisms are new records of natural enemies of CBB. This emphasizes the little knowledge about these natural control agents and competitors that can help to keep the CBB population under control. The predators were the group with higher number of natural enemies followed by the competitors, fungi, bacteria and protozoans. Only one parasitoid wasp was recorded attacking CBB adults.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beauveria bassiana; Coffee Berry Borer; Entomopathogen; Parasitoid; Predator.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2002000400018

MORTALITY OF APPLE LEAFROLLER EXPOSED TO DIFFERENT Bacillus thuringiensis SUBSPECIES IN ARTIFICIAL DIET, IN THREE ASSESSMENT PERIODS
Provedor de dados: 94
Autores: Pereira,Jonathan; Monteiro,Lino Bittencourt; Gómez,Daniel Ricardo Sosa.
ABSTRACT  Bioinsecticides based on Bacillus thuringiensis are the entomopathogen most marketed worldwide. The aim of this study was to evaluate the bioactivity of kurstaki, aizawai subspecies and kurstaki x aizawai hybrid on B. salubricola larvae fed on artificial diet in different assessment periods. Mortality was assessed on the seventh, tenth and fifteenth days after treatment. Microplates containing 150 µl of diet in each cell were used. Suspensions containing Bt subspecies was deposited on the surface of the diet, then neonate larvae were released. The mortality assessment performed after fifteen days of treatment was significant for all three subspecies. Bt kurstaki was more efficient in all assessment periods, except for fifteen days, which was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bonagota salubricola; Apple tree; Entomopathogen.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100403

Detección de Malpighamoeba mellifcae (Protista: Amoebozoa) en Apis mellifera (Hymenoptera: Apidae)de Argentina
Provedor de dados: 99
Autores: Plischuk,Santiago; Lange,Carlos E.
Debido a su rol como polinizador y productor de miel, la abeja Apis mellifera L. es considerado un insecto beneficioso. Si bien Argentina juega un papel de liderazgo en la producción de miel, existe un considerable vacío en el conocimiento acerca de las enfermedades de etiología protista que afectan las abejas en el país. La ameba Malpighamoeba mellificae Prell es un protista entomopatógeno que invade los túbulos de Malpighi de las abejas e interfiere con el proceso de excreción, debilitando al huésped y posiblemente facilitando la acción de otros patógenos. En esta contribución se presentan los primeros hallazgos de M. mellificae en Argentina y se brindan datos iniciales acerca de su frecuencia, intensidad de las infecciones, y co-ocurrencia con Nosema...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Abeja; Ameba; Entomopatógeno; Quiste; Túbulos de Malpighi; Amoeba; Cyst; Entomopathogen; Honey bee; Malpighian tubules.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802010000200017

Biological parameters of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) assayed with Bacillus thuringiensis berliner
Provedor de dados: 63
Autores: Polanczyk,Ricardo Antonio; Alves,Sérgio Batista.
The bacterium Bacillus thuringiensis (Bt) is a promising control agent of Spodoptera frugiperda (J.E. Smith). Besides the lethal effect on hosts, the entomopathogen may cause physiological alterations that hinder insects' feeding intake and reproduction. Larval and female pupa weight, oviposition and egg fecundity were determined in second instar S. frugiperda larvae infected with 39 Bt isolates in four bioassays. Sublethal effects were observed in the follow isolates: ESALQ 1.1, ESALQ 1.4, ESALQ 1.6, ESALQ 3.7, ESALQ 4.2 and ESALQ 5.2 (larval and female pupae weight); ESALQ 5.1 (larval weight); ESALQ 1.2 and ESALQ 3.7 (egg fecundity); ESALQ 3.7 (oviposition and egg fecundity); ESALQ 3.7 and ESALQ 5.2 (oviposition). Significant correlations were observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogen; Fall armyworm; Sublethal effects.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000500009

Colletotrichum nymphaeae var. entomophilum var. nov. a natural enemy of the citrus scale insect, Praelongorthezia praelonga (Hemiptera: Ortheziidae)
Provedor de dados: 63
Autores: Wynns,Anja Amtoft; Jensen,Annette Bruun; Eilenberg,Jørgen; Delalibera Júnior,Italo.
ABSTRACT: The citrus scale insect Praelongorthezia praelonga (Douglas), a major pest of citrus and other economically important crops, has only two commonly documented natural enemies: an entomopathogenic strain of the fungus Colletotrichum nymphaeae (Pass.) Aa and several parasitoids. The entomopathogenic strain of C. nymphaeae, formerly recognized under the synonym C. gloeosporioides f. sp. ortheziidae, is under development for commercial application as a biological control agent in citrus in Brazil-the top exporter of citrus globally. The synonomy of C. gloeosporioides f. sp. ortheziidae with C. nymphaeae remains based on limited DNA sequence data and without morphological study. To qualify for future approval as a biological control agent by federal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Colletotrichum (Sordariomycetes: Glomerellaceae); Entomopathogen.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000500201

Estabilidade de uma formulação de Bacillus sphaericus armazenada sob diferentes temperaturas
Provedor de dados: 63
Autores: Alves,Luis Francisco Angeli; Alves,Sérgio Batista; Lopes,Rogério Biaggioni; Augusto,Nílson Toschi.
O objetivo deste trabalho foi avaliar a estabilidade de uma formulação granulada de Bacillus sphaericus à base de sabugo de milho, armazenada em condições ambientais e a 50°C, tendo como comparação, uma suspensão concentrada de esporos e cristais da bactéria. A análise periódica dos materiais armazenados revelaram o efeito protetor da formulação sobre o microrganismo, de forma que ao longo de 12 meses de armazenamento, em condições ambiente, a viabilidade dos esporos foi maior que na suspensão concentrada. Além disso, a atividade inseticida para larvas de Culex quinquefasciatus, avaliada nos criadouros artificiais foi comparável ao patógeno não formulado. Quando mantidos a 50°C, a ação protetora da formulação mostrou-se mais eficiente, sendo que ao final...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culex quinquefasciatus; Entomopatógeno; Controle microbiano; Culex quinquefasciatus; Entomopathogen; Microbial control.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000100004

Sublethal effect of concentrations of Metarhizium anisopliae (metsch.) sorok on the larval stage and immunologic characteristics of Diatraea flavipennella (box) (lepidoptera: crambidae)
Provedor de dados: 42
Autores: GUIMARÃES,JENNIFER; MARQUES,EDMILSON J.; WANDERLEY-TEIXEIRA,VALÉRIA; ALBUQUERQUE,AURISTELA C. DE; PASSOS,ELIANA M. DOS; SILVA,CÍNTHIA C.M.; TEIXEIRA,ÁLVARO A.C.
This work aimed to evaluate the effects of Metarhizium anisopliae on Diatraea flavipennella and investigate their immune response. Was observed the effect of M. anisopliae against larvae of D. flavipennella sprayed at concentrations of 103, 104, 105 conidia / mL, in which showed differences relative the larval period, extending up to 72.0 days in treatment and 25.0 days in the control. The results for hemocytes revealed that the most frequent cells when sprayed at the concentrations of 103, 105, 107 conidia / mL were the prohemocytes, spherulocytes, plasmatocytes and granulocytes in relation to adipohemocytes and oenocytoids. The level of nitric oxide was different between the control and the concentration 107 spores / mL (24), while the activity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogen; Hemocytes; Histochemistry; Nitric oxide; Phenoloxidases; Sugarcane borer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401973

Seleção de matéria prima para a elaboração de meio de cultura para produção de Bacillus thuringiensis var. kurstaki Berliner
Provedor de dados: 44
Autores: Alves,Luis F. A.; Alves,Sérgio B.; Capalbo,Deise M. F.
The spore production "in vitro" of 43 combinations of agroindustrial by-products (cheese whey, silkworm flour, soybean protein and foder yeast) was evaluated. All media tested showed to be suitable for Bacillus thuringiensis var. kurstaki Berliner production and the concentration of spores obtained in these media varied from 10(6) to 10(9) spores/ml.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Entomopathogen; Bacteria; Culture techniques.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000200022

First report of the occurrence of Ophiocordyceps melolonthae (Ascomycota: Hypocreales: Ophiocordycipitaceae) in larvae of Diloboderus abderus Sturm (Coleoptera: Melolonthidae) in Brazil
Provedor de dados: 49
Autores: Salgado-Neto,Geraldo; Valmorbida,Ivair; Guedes,Jerson Vanderlei Carús; Blume,Elena.
This note is the first report on the infection of Diloboderus abderus Sturm (Coleoptera: Melolonthidae) larvae by the fungusOphiocordyceps melolonthae (Hypocreales: Ophiocordycipitaceae) in subtropical Brazil. Identification was made possible by extraction and sequencing of the fungal DNA that was covering the larvae’s mouthparts, prothorax, cuticle, and digestive tract (alimentary canal). Amplification, sequencing and comparison of the ITS region of the ribosomal DNA with voucher sequences of GenBank were performed and were 95% similar to Ophiocordyceps melolonthae. The fungus is an entomopathogen which attacks Melolonthidae larvae, having scientific and economic importance because of the need for increased knowledge on its distribution and on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Dissemination; Entomopathogen; Entomopathogenic fungi; Natural infection; Controle biológico; Disseminação; Entomopatógeno; Fungos entomopatogênicos; Infecção natural.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000200402

Bioassay assessment of metarhizium anisopliae (metchnikoff) sorokin (deuteromycota: hyphomycetes) against Oncometopia facialis (signoret) (hemiptera: cicadellidae)
Provedor de dados: 58
Autores: Pria Júnior,Wolney Dalla; Lacava,Paulo Teixeira; Messias,Claudio Luiz; Azevedo,João Lúcio; Lacava,Pedro Magalhães.
Citrus Variegated Chlorosis (CVC) is an economically important, destructive disease in Brazil and is caused by Xylella fastidiosa and transmitted by sharpshooter insects. In this study, the efficacy of the fungus Metarhizium anisopliae in controlling the sharpshooter Oncometopia facialis was studied by bioassay conditions. In the bioassay, insects were sprayed with a suspension containing 5 X 10(7) conidia mL-1. Adults captured in the field were treated in groups of 10 in a total of 11 replications per treatment. Significant differences between the natural mortality and the mortality of insects treated with the fungus were observed 6 days after inoculations (P&lt;0.05). These significant differences increased until 10 days after treatment. The fungus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial control; Entomopathogen; Citrus Variegated Chlorosis (CVC); Bioassay.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100027

Field persistence of Bacillus thuringiensis on maize leaves (Zea mays L.)
Provedor de dados: 58
Autores: Haddad,Marinéia de Lara; Polanczyk,Ricardo Antonio; Alves,Sergio Batista; Garcia,Marcelo de Oliveira.
The persistence slope of Bacillus thuringiensis (Bt) based products in the field is an important parameter to evaluate their efficacy. The half-life, estimated based on persistence slope parameters, is one of the most effective tools to select microbial pesticides. The aim of this research was to study the relationship between viability loss of Bt spores on maize leaves and their concentration, comparing it with field persistence. The experimental design was split-plot on time, composed by maize plants, in which three concentrations (half, normal and double doses) of a Dipel commercial formulation were applied. In each plot three leaves in the upper part of three plants where randomly selected. Samples of these leaves were collected 3 to 72 hours after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogen; Dipel; Maize leaves; Bacillus thuringiensis; Field persistence.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400001

Non-Target Effects of Dalaca pallens Blanchard Control Examined Through Principal Response Curves: A Guild Approach in Southern Chile
Provedor de dados: 117
Autores: Devotto,Luis; Cisternas,Ernesto; Carrillo,Roberto; Gerding,Marcos.
The non-target effects of biological control are a complex matter requiring a multi-approach analysis. A fungal biopesticide is under research to control the larval stages of the ghost moth Dalaca pallens Blanchard (Lepidoptera: Hepialidae) in southern Chile. A field experiment was conducted to compare the effects of the biological control agent Beauveria bassiana (Balsamo) Vuillemin (10(12) spores ha-1) and the insecticide lambda-cyhalothrin (7.5 g active ingredient ha-1) on non-target invertebrate species belonging to different guilds. The soil invertebrates were collected by using pitfall trapping and soil coring before and after spraying the biocontrol agent or the insecticide. Caught individuals were assigned to predator, herbivore or decomposer...
Tipo: Journal article
Palavras-chave: Ghost moth; Entomopathogen; Biopesticide; Lepidoptera; Hepialidae.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000300002

White grub (Coleoptera: Melolonthidae) mortality induced by Ophiocordyceps melolonthae
Provedor de dados: 65
Autores: Salgado-Neto,Geraldo; Vaz,Marcos Braz; Guedes,Jerson Vanderlei Carús.
ABSTRACT: The occurrence of white grub roots in soybean crops in the South of Brazil has gradually increased. However, there is not information on the biological control of grubs by entomopathogenic fungi. This study aimed to induce infection by Ophiocordyceps melolonthae and analyze longevity in Cyclocephala modesta and Dyscinetus gagates larvae (Coleoptera: Melolonthidae). In the laboratory, Cyclocephala modesta and Dyscinetus gagates had a mortality rate of 85% and 75%, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Dissemination; Entomopathogen; Entomopathogenic fungi; Induced infection.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600156

Relationship between chemical properties of the soil and the occurrence of Bacillus thuringiensis
Provedor de dados: 65
Autores: Polanczyk,Ricardo Antonio; Zanúncio,José Cola; Alves,Sérgio Batista.
The soil and other substrates such as mushroom compounds are the main sources of new Bacillus thuringiensis (Bt) isolates for Integrated Pest Management programs. This study describes the relationship between chemical properties of the soil (pH, OM, P3+, K1+, Ca2+, Mg2+, H1++Al3+, B3+, Cu2+, Fe2+, Mn2+ and Zn2+) and the occurrence of Bt in Brazil. A total of 1,197 bacterial colonies were obtained, being 512 of them identified as Bt. The Bt index (iBt), which is the relation between Bt colonies and bacterial counts ranged from 0.18 to 0.86. The iBt may be expressed with the formula: iBt= -0.4 + 0.6Ca2+ + 0.07Cu2+ + 0.009Fe2+ - 0.53Mg2+ -0.12Mn2+ + 1.26Zn2+. A cluster of samples with fewer colonies and a high negative correlation (antagonism) between Mn2+...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bt; Chemical elements; Entomopathogen; Soil; Biological control.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100001