Consulta: subjectFacets:"Epidermal growth factor"
Registros recuperados: 5
Data/hora: 10/06/2024 11:58:05

Assessment of molecular recognition element for the quantification of human epidermal growth factor using surface plasmon resonance
Provedor de dados: 69
Autores: Rosti,Ira Amira; Ramanan,Ramakrishnan Nagasundara; Ling,Tau Chuan; Ariff,Arbakariya B.
Background: A method for the selection of suitable molecular recognition element (MRE) for the quantification of human epidermal growth factor (hEGF) using surface plasmon resonance (SPR) is presented. Two types of hEGF antibody, monoclonal and polyclonal, were immobilized on the surface of chip and validated for its characteristics and performance in the quantification of hEGF. Validation of this analytical procedure was to demonstrate the stability and suitability of antibody for the quantification of target protein. Results: Specificity, accuracy and precision for all samples were within acceptable limit for both antibodies. The affinity and kinetic constant of antibodies-hEGF binding were evaluated using a 1:1 Langmuir interaction model. The model...
Tipo: Journal article
Palavras-chave: Antibody; BIAcore; Biosensors; Epidermal growth factor; Validation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600015

DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy
Provedor de dados: 74
Autores: Li,Deming; Fan,Jinyu; Li,Ziwei; Xu,Cunshuan.
Epidermal growth factor (EGF), a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue from control rats and partial hepatectomy (PH) rats at 10 time points was extracted and a 354 bp fragment including 10 CpG sites from the transcription start was amplified after DNA was modified by sodium bisulfate. The result of sequencing suggested that methylation ratio of four CpG sites was found to be significantly changed when PH group was compared to control group, in particular two of them were extremely striking. mRNA expression of EGF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylation dynamics; Epidermal growth factor; Liver regeneration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300017

Autonomous growth of BALB/MK keratinocytes transfected   with a retroviral vector carrying the human epidermal growth factor gene
Provedor de dados: 74
Autores: Choudhuri,Jomuna V.; Mathor,Monica B.; Silva,Flávia H.; Han,Sang W.
Epidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important for the proliferation and differentiation of epidermal and epithelial tissues in animals. Exogenous EGF is a promising therapeutic agent for wound healing, but its general use is restricted by the limited availability of this protein. In this work, we show that the transfection of mouse BALB/MK keratinocytes, which are totally dependent on EGF for growth and migration, with mature cDNA for human EGF via a retroviral vector abolished the cells requirement for exogenous EGF. The transformed cells had normal morphology and a growth rate that varied according to the source of the retroviral vector used. Keratinocyte transfection with EGF cDNA provides a time-...
Palavras-chave: BALB/MK keratinocytes; Epidermal growth factor; Retroviral vector.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500003

Effects of FGF-2 and EGF removal on the differentiationof mouse neural precursor cells
Provedor de dados: 42
Autores: Schwindt,Telma T.; Motta,Fabiana L.; Gabriela F.,Barnabé; Cristina G.,Massant; Guimarães,Alessander O.; Calcagnotto,Maria Elisa; Pesquero,João B.; Mello,Luiz E.
Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neural precursor cells; Neurosphere; Fibroblast growth factor 2; Epidermal growth factor; Differentiation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300009

Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
Provedor de dados: 48
Autores: AVILA,ANA D; CALDERÓN,CARLOS F; PEREZ,RITA M; PONS,CARMEN; PEREDA,CELIA M; ORTIZ,ANA R.
Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that...
Tipo: Journal article
Palavras-chave: Epidermal growth factor; Epidermal growth factor receptor; Hemolytic toxins; Immunotoxins; Monoclonal antibodies.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200008