Consulta: subjectFacets:"Ex situ conservation"
Registros recuperados: 17
Data/hora: 09/06/2024 08:22:50

Propagación sexual y tolerancia a la desecación en nanche (Byrsonima crassifolia L.) Kunth.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Jaimes Albíter, Camelia.
El nanche es una especie forestal frutícola de amplio espectro utilitario, pero la germinación de sus semillas es compleja; por ello, el objetivo de la investigación fue conocer la dinámica germinativa de las semillas de clima cálido húmedo y clima semicálico, en el banco del suelo y en condiciones de invernadero; así como clasificarlas fisiológicamente, y proponer alternativas adecuadas para conservar este recurso fitogenético. Se encontró que las semillas de clima cálido húmedo forman un banco de tipo transitorio, en tanto que, para las de clima semicálido fue persistente de corto plazo. Por otro lado, en invernadero se observó que la humedad del suelo no representa inconveniente para la germinación de las semillas de ambos climas, si no que esto depende...
Palavras-chave: Byrsonima crassifolia; Latencia; Recalcitrante; Conservación in situ; Conservación ex situ; Dormancy; In situ conservation; Ex situ conservation; Producción de Semillas; Doctorado; Recalcitrant.
Ano: 2013
URL: http://hdl.handle.net/10521/2179

Procedimentos para avaliar a predação, dormência, germinação e conservação de sementes de Tachigali subvelutina (Benth.) Oliveira-Filho.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: ALVES-DA-SILVA, D.; BENITO, N. P.
Tachigali subvelutina, conhecida popularmente como veludo, é uma árvore nativa do Cerrado cuja madeira é tradicionalmente utilizada no norte de Minas Gerais. Nesse trabalho foram descritos aspectos importantes para a promoção da sua conservação. A porcentagem da predação pré-dispersão das sementes foi quantificada, foi verificado se as sementes apresentavam dormência, se a escarificação aumentava a porcentagem de germinação, além de testar se elas eram tolerantes à dessecação, ao resfriamento (4º C) e ao congelamento (-8º C). Também foi avaliado se a utilização de embalagens de leite recicladas pode ser uma alternativa para a conservação ex situ das sementes em geladeira ou congelador dentro de uma realidade rural. Os experimentos de germinação foram...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Veludo; Escarificação; Conservação ex situ; Overcoming dormancy; Scarification; Ex situ conservation.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1131697

Germinação, tolerância à dessecação e ao congelamento de sementes de Vellozia squamata Pohl: germinação e conservação ex situ da canela-de-ema.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: SILVA, D. A. da.
bitstream/item/225477/1/Boletim-caneladeema-3731.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Canela-de-ema; Conservação ex situ; Cerrado; Conservação; Velloziaceae; Ex situ conservation.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1133855

Is the genetic integrity of natural plant populations ex situ preserved with the current sampling, conservation and regeneration approaches?
Provedor de dados: Journal of Basic and Applied Genetics (BAG)
Autores: Camadro,E.L.
Acceleration of environment degradation and climate change poses serious threats to both natural and agricultural ecosystems. In fact, the number of endangered plant species at the global level has increased yearly in the last decades, due to anthropic interventions that sum up to the climate change. Samples of natural plant populations, particularly of wild crop relatives, are ex situ conserved in germplasm banks, which have become active in the provision of genetic resources to breeders. The aim of this article is to generate discussion on the adequacy of (a) current protocols for sampling and ex situ conservation of the natural genetic diversity and (b) the actual species paradigm in plants, due to their consequences for this and future generations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological species; Ex situ conservation; Ex situ regeneration; Plant genetic resources; Taxonomic species.
Ano: 2014
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332014000100005

Estimates of genetic parameters and variability in provenances and progeny of Handroanthus vellosoi
Provedor de dados: PFB - Pesquisa Florestal Brasileira
Autores: Batista, Camila Moreira; Freitas, Miguel Luiz Menezes; Moraes, Marcela Aparecida de; Zanatto, Antonio Carlos Scatena; Santos, Pedro César dos; Zanata, Marcelo; Moraes, Mario Luiz Teixeira de; Sebbenn, Alexandre Magno.
The present study aimed at estimating genetic parameters and variability among and within provenances for silvicultural traits of Handroanthus vellosoi (Toledo) Mattos, in provenances and progenies test stands, located at the Luiz Antônio Experimental Station (São Paulo State, Brazil). The test was established in the compact family block design, with two provenances (allocated in the plots), six replicates and sub-linear plots of five plants, totalizing 35 progenies. Diameter at breast height (DBH), plant height, cylindrical volume, stem form and survival at 24 years of age were evaluated. Significant differences among the provenances were observed only to the trait stem form. The analysis of deviance showed significant differences among progenies for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ex situ conservation; Tropical tree species; Population genetics; Quantitative genetics Recursos genéticos florestais; Conservação genética; Genética de populações; Genética quantitativa; Melhoramento genético florestal Conservação ex situ; Espécies arbóreas tropicais; Genética de populações; Genética quantitativa.
Ano: 2012
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/358

In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Gomes,Hugo Teixeira; Bartos,Patrícia Monah Cunha; Andrade,Maíra Teixeira de; Almeida,Raphael Ferreira; Lacerda,Luciana Florencio de; Scherwinski-Pereira,Jonny Everson.
Abstract: The objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rubus; Ex situ conservation; Plant genetic resources; Plant propagation; Slow-growth.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001201286

In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Silva,Tatiane Loureiro da; Scherwinski-Pereira,Jonny Everson.
The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ex situ conservation; Germplasm bank; Micropropagation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400007

Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Lédo,Ana da Silva; Jenderek,Maria; Skogerboe,Dianne; Staats,Elise; Machado,Caroline de Araújo; Oliveira,Leila Albuquerque Resende de.
Abstract: The objective of this work was to evaluate rewarming procedures and recovery media for Brazilian Green Dwarf coconut (Cocos nucifera) embryos cryopreserved by vitrification. The rewarming procedures evaluated were: T1, water bath at 38±2°C; and T2, unloading solution consisting of Y3 medium + 1.2 mol L-1 sucrose. The recovery media assessed were: M1, Y3 medium + 45 g L-1 sucrose + 1 mg L-1 gibberellic acid + 1 g L-1 activated charcoal + 2.2 g L-1 Gelrite; and M2, Y3 medium + 60 g L-1 sucrose + 2.2 g L-1 Gelrite. Rewarming procedures showed no significant effect, and the M1 media induced 72.5% regeneration.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucifera; Ex situ conservation; PVS3.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000400514

Short-term storage in vitro and large-scale propagation of grapevine genotypes
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Silva,Rafael de Carvalho; Luis,Zanderluce Gomes; Scherwinski-Pereira,Jonny Everson.
The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis; Germplasm; Ex situ conservation; In vitro conservation; Micropropagation; Minimal growth.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000300005

In vitro conservation of Cape gooseberry through slow-growth nodal segment cultures
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Rezende,Renata Alves Lara Silva; Rodrigues,Filipe Almendagna; Rezende,Ramiro Machado; Soares,Joyce Dória Rodrigues; Pasqual,Moacir; Assis,Franscinely Aparecida de.
Abstract: The objective of this work was to evaluate the effects of temperature and osmotic agents on the in vitro conservation of Cape gooseberry (Physalis peruviana). Temperatures at 18 and 25°C, as well as the osmotic agents sucrose, mannitol, and sorbitol were tested. A short-term in vitro conservation of Cape gooseberry can be achieved at 18°C, using 30 g L-1 sucrose.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physalis peruviana; Ex situ conservation; Tissue culture.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500651

Crop Genetic Resources:  An Economic Appraisal
Provedor de dados: Research in Agricultural & Applied Economics (AgEcon Searh)
Autores: Day-Rubenstein,   Kelly A.; Heisey,   Paul W.; Shoemaker,   Robbin A.; Sullivan,   John; Frisvold,   George B.
Crop genetic resources are the basis of agricultural production, and significant economic benefits have resulted from their conservation and use. However, crop genetic resources are largely public goods, so private incentives for genetic resource conservation may fall short of achieving public objectives. Within the U.S. germplasm system, certain crop collections lack sufficient diversity to reduce vulnerability to pests and diseases. Many such genetic resources lie outside the United States. This report examines the role of genetic resources, genetic diversity, and efforts to value genetic resources. The report also evaluates economic and institutional factors influencing the flow of genetic resources, including international agreements, and their...
Tipo: Report
Palavras-chave: Genetic resources; Genetic diversity; Germplasm; R&amp;D; International transfer of genetic resources; In situ conservation; Ex situ conservation; Gene banks; Intellectual property.; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2005
URL: http://purl.umn.edu/59388

Morphological characterization of active germoplasm bank fig tree accessions
Provedor de dados: Revista Brasileira de Fruticultura
Autores: Rodrigues,Maria Gabriela Fontanetti; Santos,Tatiane Paes dos; Ferreira,Antonio Flávio Arruda; Monteiro,Laís Naiara Honorato; Nakanishi,Eduarda Sayuri; Boliani,Aparecida Conceição.
Abstract  Fig tree (Ficus carica L.) is a fruit of great importance worldwide, being able to be implanted as new source of agricultural variability. The aim of the present work was to characterize the active germplasm bank of fig trees by means of morphological descriptors of 45 fig accessions at the Faculty of Agrarian and Technological Sciences, Campus of Dracena, FCAT / UNESP aiming at the recognition of the quality and genetic variability potential of the species. Thus, their biometric characteristics were evaluated by qualitative and quantitative descriptors of leaves and fruits. Fig tree accessions were characterized by means of the morphological characteristics of leaves and fruits, identifying AGB with high phenotypic variability and well adapted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ficus carica L.; Ex situ conservation; Genetic resources; Biometrics.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000500802

Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: DEMARCHI,GRASIELA; STEFENON,VALDIR M.; STEINER,NEUSA; VIEIRA,FELIPE N.; VESCO,LIRIO L. DAL; GUERRA,MIGUEL P.
This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Ex situ conservation; In vitro culture; Plant germplasm conservation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057

Tropical seed species' responses to liquid nitrogen exposure
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Salomão,Antonieta Nassif.
The ability to tolerate ultra-low temperature (-196 ºC) exposure was evaluated in 66 tropical orthodox seed species of 21 botanical families from the Cerrado (Brazilian Savannah) and Atlantic Forest Brazilian biomes. Liquid nitrogen had no effect on the germinability of 51 seed species. The stimulatory effect of cryogenic temperature on germinability, with or without subsequent chemical scarification, was observed in nine seed species with deep physical dormancy, or heterogeneous levels of seed hardness, or with no dormancy. Significant reduction in germinability occurred in six seed species, presumably because of factors acting individually or in combination on these seeds, such as inappropriate moisture content, or the potentially detrimental effect of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Ex situ conservation; Seed dormancy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000200008

Strategies for exchange of coconut germplasm in Brazil
Provedor de dados: Ciência Rural
Autores: Lédo,Ana da Silva; Oliveira,Leila Albuquerque Resende de; Machado,Caroline de Araújo; Silva,Ana Veruska Cruz da; Ramos,Semíramis Rabelo Ramalho.
ABSTRACT: Seed size and its physiology are a barrier for the exchange of coconut genetics resources and the tissue culture techniques can assist the germplasm bank enrichment. The aim of this study was to evaluate the effect of transport and storage procedures of zygotic embryos to promote the exchange of coconut accessions. Mature zygotic embryo from Cameroon red dwarf (CRD), Malayan yellow dwarf (MYD) and Malayan red dwarf (MRD) were used. The following treatments were evaluated: T1-storage of endosperm disc in plastic bags at 10±2°C for 5 days; T2 - 8 days; T3 - 12 days; T4 - embryo excised and inoculated in micro tube with Y3 culture medium with no sucrose for 2 days and T5 - five embryos inoculated in Y3 culture medium without sucrose in Petri dish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucifera; Ex situ conservation; Genetic resources; Contamination.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300408

Cryopreservation of coffee zygotic embryos: dehydration and osmotic rehydration
Provedor de dados: Ciência e Agrotecnologia
Autores: Pinto,Maísa de Siqueira; Paiva,Renato; Silva,Diogo Pedrosa Corrêa da; Santos,Paulo Augusto Almeida; Freitas,Rodrigo Therezan de; Silva,Luciano Coutinho.
ABSTRACT Conservation of plant genetic resources is important to prevent genetic erosion. Seed banks are the most common method of ex situ conservation; however, coffee seeds can not be stored by conventional methods. Cryopreservation is a viable alternative for long-term conservation of species that produce intermediate or recalcitrant seeds, as coffee. The aim of this work was to cryopreserve Coffea arabica L. cv Catuaí Vermelho IAC 144 zygotic embryos, and analyse the effects of dehydration prior cryopreservation and osmotic rehydration after thawing, in embryos germination and seedlings formation after cryopreservation. Prior to cryopreservation, different dehydration times (0, 15, 30, 60 and 120 min) were tested. Dehydrated embryos were cryopreserved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica L; Ex situ conservation; Embryos dehydration; Long-term storage; Gene bank.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000400380

Orchid cryopreservation
Provedor de dados: Ciência e Agrotecnologia
Autores: Vendrame,Wagner; Faria,Ricardo Tadeu de; Sorace,Mauren; Sahyun,Sandra Aparecida.
Orchids are lush and highly valuable plants due to their diversity and the beauty of their flowers, which increases their commercialization. The family Orchidaceae comprises approximately 35,000 species, distributed among more than 1,000 distinct genera and 100,000 hybrids, totaling approximately 8% to 10% of all flowering plants. With the advance of agriculture and the constant destruction of their natural habitat, orchid species are collected in an indiscriminate manner by collectors and vendors, and this extractive activity threatens many species with extinction, drastically reducing their genetic variability in nature. Therefore, it is essential to seek alternatives that make the preservation of such species feasible using techniques with low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preservation; Orchidaceae; Ex situ conservation; Freezing tolerance; Extinction.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000300001